DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 188 |
| Acc65I | GGTACC | 1 cut(s) | 135 |
| AccB1I | GGYRCC | 1 cut(s) | 135 |
| AccII | CGCG | 3 cut(s) | 59, 141, 265 |
| AciI | CCGC | 8 cut(s) | 59, 139, 169, 265, 296, 299, 302, 519 |
| AcyI | GRCGYC | 2 cut(s) | 72, 185 |
| AfaI | GTAC | 1 cut(s) | 137 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 174, 225, 497 |
| AjiI | CACGTC | 1 cut(s) | 342 |
| Alw21I | GWGCWC | 1 cut(s) | 69 |
| Alw26I | GTCTC | 1 cut(s) | 448 |
| AoxI | GGCC | 4 cut(s) | 127, 215, 266, 378 |
| ApeKI | GCWGC | 1 cut(s) | 469 |
| Asp718I | GGTACC | 1 cut(s) | 135 |
| AspA2I | CCTAGG | 1 cut(s) | 130 |
| AspLEI | GCGC | 2 cut(s) | 59, 469 |
| AspS9I | GGNCC | 3 cut(s) | 165, 216, 267 |
| AsuHPI | GGTGA | 2 cut(s) | 383, 487 |
| AvaII | GGWCC | 1 cut(s) | 165 |
| AvrII | CCTAGG | 1 cut(s) | 130 |
| BanI | GGYRCC | 1 cut(s) | 135 |
| BbsI | GAAGAC | 1 cut(s) | 236 |
| Bbv12I | GWGCWC | 1 cut(s) | 69 |
| BbvI | GCAGC | 1 cut(s) | 481 |
| BccI | CCATC | 5 cut(s) | 83, 197, 304, 362, 525 |
| BceAI | ACGGC | 2 cut(s) | 142, 309 |
| BcoDI | GTCTC | 1 cut(s) | 448 |
| BfaI | CTAG | 1 cut(s) | 131 |
| BisI | GCNGC | 5 cut(s) | 266, 297, 300, 303, 470 |
| BlnI | CCTAGG | 1 cut(s) | 130 |
| BlsI | GCNGC | 5 cut(s) | 267, 298, 301, 304, 471 |
| Bme18I | GGWCC | 1 cut(s) | 165 |
| BmgBI | CACGTC | 1 cut(s) | 342 |
| BmgT120I | GGNCC | 3 cut(s) | 165, 216, 267 |
| BmiI | GGNNCC | 3 cut(s) | 38, 137, 167 |
| BpiI | GAAGAC | 1 cut(s) | 236 |
| BpmI | CTGGAG | 1 cut(s) | 23 |
| Bsa29I | ATCGAT | 1 cut(s) | 20 |
| BsaHI | GRCGYC | 2 cut(s) | 72, 185 |
| BsaJI | CCNNGG | 3 cut(s) | 46, 130, 172 |
| BsaWI | WCCGGW | 1 cut(s) | 497 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 245, 275 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 174, 225, 497 |
| Bse118I | RCCGGY | 2 cut(s) | 346, 472 |
| Bse1I | ACTGG | 1 cut(s) | 167 |
| Bse3DI | GCAATG | 2 cut(s) | 78, 312 |
| BseCI | ATCGAT | 1 cut(s) | 20 |
| BseDI | CCNNGG | 3 cut(s) | 46, 130, 172 |
| BseGI | GGATG | 3 cut(s) | 199, 373, 415 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 174, 225, 497 |
| BseMI | GCAATG | 2 cut(s) | 78, 312 |
| BseMII | CTCAG | 1 cut(s) | 414 |
| BseNI | ACTGG | 1 cut(s) | 167 |
| BseRI | GAGGAG | 3 cut(s) | 110, 475, 493 |
| BseXI | GCAGC | 1 cut(s) | 481 |
| BseYI | CCCAGC | 1 cut(s) | 218 |
| Bsh1236I | CGCG | 3 cut(s) | 59, 141, 265 |
| Bsh1285I | CGRYCG | 1 cut(s) | 24 |
| BshFI | GGCC | 4 cut(s) | 129, 217, 268, 380 |
| BshNI | GGYRCC | 1 cut(s) | 135 |
| BshVI | ATCGAT | 1 cut(s) | 20 |
| BsiEI | CGRYCG | 1 cut(s) | 24 |
| BsiHKAI | GWGCWC | 1 cut(s) | 69 |
| BsiSI | CCGG | 3 cut(s) | 347, 473, 498 |
| BslFI | GGGAC | 1 cut(s) | 119 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 174, 225, 497 |
| BsmAI | GTCTC | 1 cut(s) | 448 |
| BsmBI | CGTCTC | 1 cut(s) | 448 |
| BsmFI | GGGAC | 1 cut(s) | 119 |
| BsnI | GGCC | 4 cut(s) | 129, 217, 268, 380 |
| Bsp1286I | GDGCHC | 1 cut(s) | 69 |
| Bsp143I | GATC | 2 cut(s) | 21, 492 |
| BspACI | CCGC | 8 cut(s) | 59, 139, 169, 265, 296, 299, 302, 519 |
| BspANI | GGCC | 4 cut(s) | 129, 217, 268, 380 |
| BspCNI | CTCAG | 1 cut(s) | 415 |
| BspDI | ATCGAT | 1 cut(s) | 20 |
| BspFNI | CGCG | 3 cut(s) | 59, 141, 265 |
| BspLI | GGNNCC | 3 cut(s) | 38, 137, 167 |
| BspT107I | GGYRCC | 1 cut(s) | 135 |
| BsrDI | GCAATG | 2 cut(s) | 78, 312 |
| BsrFI | RCCGGY | 2 cut(s) | 346, 472 |
| BsrI | ACTGG | 1 cut(s) | 167 |
| BssAI | RCCGGY | 2 cut(s) | 346, 472 |
| BssECI | CCNNGG | 3 cut(s) | 46, 130, 172 |
| BssMI | GATC | 2 cut(s) | 21, 492 |
| BssNI | GRCGYC | 2 cut(s) | 72, 185 |
| BssT1I | CCWWGG | 1 cut(s) | 130 |
| Bst4CI | ACNGT | 1 cut(s) | 235 |
| Bst6I | CTCTTC | 1 cut(s) | 150 |
| BstACI | GRCGYC | 2 cut(s) | 72, 185 |
| BstC8I | GCNNGC | 1 cut(s) | 474 |
| BstDEI | CTNAG | 1 cut(s) | 423 |
| BstDSI | CCRYGG | 1 cut(s) | 172 |
| BstF5I | GGATG | 3 cut(s) | 199, 373, 415 |
| BstFNI | CGCG | 3 cut(s) | 59, 141, 265 |
| BstHHI | GCGC | 2 cut(s) | 59, 469 |
| BstKTI | GATC | 2 cut(s) | 24, 495 |
| BstMAI | GTCTC | 1 cut(s) | 448 |
| BstMBI | GATC | 2 cut(s) | 21, 492 |
| BstMCI | CGRYCG | 1 cut(s) | 24 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 80, 147, 274, 302, 473 |
| BstNSI | RCATGY | 1 cut(s) | 528 |
| BstUI | CGCG | 3 cut(s) | 59, 141, 265 |
| BstV1I | GCAGC | 1 cut(s) | 481 |
| BstV2I | GAAGAC | 1 cut(s) | 236 |
| Bsu15I | ATCGAT | 1 cut(s) | 20 |
| BsuRI | GGCC | 4 cut(s) | 129, 217, 268, 380 |
| BsuTUI | ATCGAT | 1 cut(s) | 20 |
| BtgI | CCRYGG | 1 cut(s) | 172 |
| BtrI | CACGTC | 1 cut(s) | 342 |
| BtsCI | GGATG | 3 cut(s) | 199, 373, 415 |
| Cac8I | GCNNGC | 1 cut(s) | 474 |
| CfoI | GCGC | 2 cut(s) | 59, 469 |
| Cfr10I | RCCGGY | 2 cut(s) | 346, 472 |
| Cfr13I | GGNCC | 3 cut(s) | 165, 216, 267 |
| ClaI | ATCGAT | 1 cut(s) | 20 |
| CseI | GACGC | 1 cut(s) | 80 |
| Csp6I | GTAC | 1 cut(s) | 136 |
| CviAII | CATG | 1 cut(s) | 525 |
| CviJI | RGCY | 9 cut(s) | 37, 129, 178, 217, 268, 277, 325, 380, 472 |
| CviKI_1 | RGCY | 9 cut(s) | 37, 129, 178, 217, 268, 277, 325, 380, 472 |
| CviQI | GTAC | 1 cut(s) | 136 |
| DdeI | CTNAG | 1 cut(s) | 423 |
| DpnI | GATC | 2 cut(s) | 23, 494 |
| DpnII | GATC | 2 cut(s) | 21, 492 |
| Eam1104I | CTCTTC | 1 cut(s) | 150 |
| EarI | CTCTTC | 1 cut(s) | 150 |
| Eco130I | CCWWGG | 1 cut(s) | 130 |
| Eco47I | GGWCC | 1 cut(s) | 165 |
| EcoT14I | CCWWGG | 1 cut(s) | 130 |
| ErhI | CCWWGG | 1 cut(s) | 130 |
| Esp3I | CGTCTC | 1 cut(s) | 448 |
| FaeI | CATG | 1 cut(s) | 528 |
| FaiI | YATR | 2 cut(s) | 212, 526 |
| FaqI | GGGAC | 1 cut(s) | 119 |
| FatI | CATG | 1 cut(s) | 524 |
| FauI | CCCGC | 1 cut(s) | 176 |
| Fnu4HI | GCNGC | 5 cut(s) | 266, 297, 300, 303, 470 |
| FokI | GGATG | 3 cut(s) | 206, 380, 422 |
| Fsp4HI | GCNGC | 5 cut(s) | 266, 297, 300, 303, 470 |
| FspBI | CTAG | 1 cut(s) | 131 |
| GlaI | GCGC | 2 cut(s) | 58, 468 |
| GluI | GCNGC | 5 cut(s) | 266, 297, 300, 303, 470 |
| GsaI | CCCAGC | 1 cut(s) | 222 |
| GsuI | CTGGAG | 1 cut(s) | 23 |
| HaeIII | GGCC | 4 cut(s) | 129, 217, 268, 380 |
| HapII | CCGG | 3 cut(s) | 347, 473, 498 |
| HgaI | GACGC | 1 cut(s) | 80 |
| HhaI | GCGC | 2 cut(s) | 59, 469 |
| Hin1I | GRCGYC | 2 cut(s) | 72, 185 |
| Hin1II | CATG | 1 cut(s) | 528 |
| Hin6I | GCGC | 2 cut(s) | 57, 467 |
| HinP1I | GCGC | 2 cut(s) | 57, 467 |
| HinfI | GANTC | 2 cut(s) | 197, 260 |
| HpaII | CCGG | 3 cut(s) | 347, 473, 498 |
| HphI | GGTGA | 2 cut(s) | 383, 487 |
| Hpy166II | GTNNAC | 1 cut(s) | 339 |
| Hpy188I | TCNGA | 5 cut(s) | 183, 193, 202, 436, 492 |
| Hpy188III | TCNNGA | 2 cut(s) | 25, 40 |
| Hpy8I | GTNNAC | 1 cut(s) | 339 |
| Hpy99I | CGWCG | 7 cut(s) | 74, 125, 128, 190, 193, 334, 346 |
| HpyAV | CCTTC | 3 cut(s) | 238, 244, 250 |
| HpyCH4III | ACNGT | 1 cut(s) | 235 |
| HpyCH4IV | ACGT | 2 cut(s) | 185, 341 |
| HpyCH4V | TGCA | 1 cut(s) | 524 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 80, 147, 274, 302, 473 |
| HpyF3I | CTNAG | 1 cut(s) | 423 |
| HpySE526I | ACGT | 2 cut(s) | 185, 341 |
| Hsp92I | GRCGYC | 2 cut(s) | 72, 185 |
| Hsp92II | CATG | 1 cut(s) | 528 |
| HspAI | GCGC | 2 cut(s) | 57, 467 |
| KpnI | GGTACC | 1 cut(s) | 139 |
| KroI | GCCGGC | 1 cut(s) | 472 |
| KroNI | GCCGGC | 1 cut(s) | 474 |
| Kzo9I | GATC | 2 cut(s) | 21, 492 |
| LmnI | GCTCC | 1 cut(s) | 42 |
| LpnPI | CCDG | 7 cut(s) | 53, 148, 208, 232, 360, 486, 511 |
| Lsp1109I | GCAGC | 1 cut(s) | 481 |
| MaeI | CTAG | 1 cut(s) | 131 |
| MaeII | ACGT | 2 cut(s) | 185, 341 |
| MaeIII | GTNAC | 3 cut(s) | 25, 285, 371 |
| MalI | GATC | 2 cut(s) | 23, 494 |
| MboI | GATC | 2 cut(s) | 21, 492 |
| MboII | GAAGA | 3 cut(s) | 167, 241, 253 |
| MhlI | GDGCHC | 1 cut(s) | 69 |
| MluCI | AATT | 1 cut(s) | 280 |
| MlyI | GAGTC | 1 cut(s) | 269 |
| MmeI | TCCRAC | 2 cut(s) | 171, 414 |
| MroNI | GCCGGC | 1 cut(s) | 472 |
| MseI | TTAA | 2 cut(s) | 354, 504 |
| MslI | CAYNNNNRTG | 1 cut(s) | 529 |
| MspI | CCGG | 3 cut(s) | 347, 473, 498 |
| MvnI | CGCG | 3 cut(s) | 59, 141, 265 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 80, 147, 274, 302, 473 |
| NaeI | GCCGGC | 1 cut(s) | 474 |
| NdeII | GATC | 2 cut(s) | 21, 492 |
| NgoMIV | GCCGGC | 1 cut(s) | 472 |
| NlaIII | CATG | 1 cut(s) | 528 |
| NlaIV | GGNNCC | 3 cut(s) | 38, 137, 167 |
| NmuCI | GTSAC | 3 cut(s) | 25, 285, 371 |
| NspI | RCATGY | 1 cut(s) | 528 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 60, 120, 338 |
| PdiI | GCCGGC | 1 cut(s) | 474 |
| PfeI | GAWTC | 1 cut(s) | 197 |
| PflFI | GACNNNGTC | 1 cut(s) | 187 |
| PkrI | GCNGC | 5 cut(s) | 267, 298, 301, 304, 471 |
| Ple19I | CGATCG | 1 cut(s) | 24 |
| PleI | GAGTC | 1 cut(s) | 268 |
| PpsI | GAGTC | 1 cut(s) | 268 |
| PspFI | CCCAGC | 1 cut(s) | 218 |
| PspN4I | GGNNCC | 3 cut(s) | 38, 137, 167 |
| PspPI | GGNCC | 3 cut(s) | 165, 216, 267 |
| PsyI | GACNNNGTC | 1 cut(s) | 187 |
| PvuI | CGATCG | 1 cut(s) | 24 |
| RsaI | GTAC | 1 cut(s) | 137 |
| RsaNI | GTAC | 1 cut(s) | 136 |
| RseI | CAYNNNNRTG | 1 cut(s) | 529 |
| SaqAI | TTAA | 2 cut(s) | 354, 504 |
| SatI | GCNGC | 5 cut(s) | 266, 297, 300, 303, 470 |
| Sau3AI | GATC | 2 cut(s) | 21, 492 |
| Sau96I | GGNCC | 3 cut(s) | 165, 216, 267 |
| SchI | GAGTC | 1 cut(s) | 269 |
| SduI | GDGCHC | 1 cut(s) | 69 |
| SetI | ASST | 4 cut(s) | 52, 188, 344, 444 |
| SgrAI | CRCCGGYG | 1 cut(s) | 346 |
| SinI | GGWCC | 1 cut(s) | 165 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 529 |
| Sse9I | AATT | 1 cut(s) | 280 |
| SsiI | CCGC | 8 cut(s) | 59, 139, 169, 265, 296, 299, 302, 519 |
| SspMI | CTAG | 1 cut(s) | 131 |
| StyI | CCWWGG | 1 cut(s) | 130 |
| TaaI | ACNGT | 1 cut(s) | 235 |
| TaiI | ACGT | 2 cut(s) | 188, 344 |
| TaqI | TCGA | 4 cut(s) | 20, 114, 332, 529 |
| TasI | AATT | 1 cut(s) | 280 |
| TauI | GCSGC | 4 cut(s) | 268, 299, 302, 305 |
| TfiI | GAWTC | 1 cut(s) | 197 |
| Tru1I | TTAA | 2 cut(s) | 354, 504 |
| Tru9I | TTAA | 2 cut(s) | 354, 504 |
| TseFI | GTSAC | 3 cut(s) | 25, 285, 371 |
| TseI | GCWGC | 1 cut(s) | 469 |
| Tsp45I | GTSAC | 3 cut(s) | 25, 285, 371 |
| TspDTI | ATGAA | 2 cut(s) | 100, 210 |
| TspGWI | ACGGA | 1 cut(s) | 472 |
| Tth111I | GACNNNGTC | 1 cut(s) | 187 |
| VpaK11BI | GGWCC | 1 cut(s) | 165 |
| XceI | RCATGY | 1 cut(s) | 528 |
| XmaJI | CCTAGG | 1 cut(s) | 130 |
| XspI | CTAG | 1 cut(s) | 131 |
| ZraI | GACGTC | 1 cut(s) | 186 |