Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00860

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Reverse (-)

21277 .. 31197

Gene Structure 9921 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G019110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4617 bp

ATGGCGGACGACGAGCAACCGATCGTGACAGGGAGGCGCCGGAGCCGAGGAGCCGAGGCTTCGGCGCGGTCCAGCGCCCTGGAGCGCCTCCGAGCTCTCCGGCAAGGCGGCCGTCGCTCTGAAAACGCCGACGACCGGTTCCAGATCAAGATGGAGAAGCCGATCTACGACACGGTCGATGAGGACGAGTACGATGCTATCATCGCCAAGCGCCGCGAGGAGTTCGAGGACTTCATTGAGGACGTCGACGGCCTAGGGTACTGCGACGACGGCGAGGAAGAGGACTGGACCAGCCTCGGGCTTTCGACGTCATCGGATGAATCAAATGGCGATATGAGGCCCAGGAGGAGGAAGAAGGTGGAGAAGAATCAGAAAGAGCCGCAGCCCAAAAAGCCCAATTCGTTGCTTACGGCGGCGGCGGCGATGATGGGGAAGCAGAGGCTTTCGTCGATGTTCACGTCGTCGGTGTTTAATAAAAGTAGGGATGGTGATAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCCGAGTTTGCGCCGGACGAGGCTGATAGAGAGAAACGGAGGAGGGCACAGCCGGCAAGGAGTTTCGTTCCGATTGCCGGTGTGAAGAGTGAGAGAGTGAGTAATGTGGGTGTTGAGTTATCAAGGAGGCTTGAATTGGATAGAATTGTTGCAAATGGTGGTGGTTCTGTGCATAGTAAGGAAATGGATAAAGATTCTGAGGGCAAGAAGGAGTTGGATTTCGGAAATTGTCAGCCCCAAATTGAGCAATTCCATGATTTTTTGGAGAATAGTGAGGTGCCCGAGGAGAAGAAGACTGAGGATTCTGCTGAGGTGCAGGCGGAGCCTGTGGTGAAAAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATGAGGATCCGGCATTGAGTGCTATGGCAGGATGGAAGGCCGCGATGACTGGTGGGGCCGGGGTTACTGGTGGCGGCGGCAATGTGGAGGTCAAAAGTGACTCAATTAGTGAAGGGAGTTCGGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAATATGGGCACTGTTTATCTATTTGGGAAGGTTAAAGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTAAAGAACATGCAAAGATGTGTCTATGCAATTCCGGACAGTCCTTTATTTCAAACTGACGAAATGATGAAGCTTGAAAAGGATGCCGAGGACTCGAGGATTTCACCCACCGATTTTCGTAAGAAGTTGCATGATGCGGCTTCAGAATTAAAGAATGAATTAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGGCACCTGTCAAGAGGAACTATGCTTTTGAGCGATCTGACATACCTGCAGGGGAGAATTATGTACTGAAGATCAATTATCCATTCCGGGATCCACCACTATCAGCAGATCTAAAAGGAGAAACTTTTTGTGCTCTTCTAGGAACTCATAGCAGCGCATTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAAATTTTTCCATCTGCCCAGCTCCACAACGGGTAAGCTGGTGCAAATTTGAGGTGATCGTGAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACAAAGAAAACTGTAGATATTCCTCAAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTATCAATGAGAAGCAGAATGTTAACGAAATAGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCCGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGTGGCATATTTCCTATGGGGTTTACTAAAGTGGCTGCAGATAAGAATTCTAAAGCCGGAACAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTAAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTTGTTGGACATAATATATCTGGATTTGATCTGGATGTTCTTCTCCACAGAGCTCAGGTTTGCAGAGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATTATGTCCTGCATTGCTGGTCGGCTGTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAAGGAGGTCAGCTATTCCTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCGCATGATATTCCTAGAATGTTACAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCCTGGTTGTCTATGGGACTCATGTTTCATTTGAGTGCTCTTCCCCTCACACGTCAGCTGACTAATATAAGTGGCAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTAGAATATCTCTTACTGCATGCTTTTCATGCAAAAAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGTGTTGAGGAAAAAGCTGCTGATGAGTTGGATGTAAATGATGTAAATGTTGATAATGATGCACAGAGTGGGCATGGAAAAAGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTATTGGAGCCAAAAAAAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATATGCTTCACAACTGTTGAAAGAAATTCAGAAGGATTGGTTTCTCATTTACCTTCTAGTCAAAGACCTGGACTTTTACCTGAGTTGTTAAAAAATTTAGTTGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAACTTGACATTCAACAGCAAGCTCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAACTCAAGATTTTATGCAAAGCCACTGGCAGAGCTTATAACACTACGGGGCCGGGAGATCTTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTAATCTATGGTGATACGGACTCAATAATGATTTATAGTGGGCTGGATGATAGTGTGAAAGCAAAAGCAATTGCTGTGAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGACGGTTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCGGCTGTGAAGGAGCTAATGAAAAACGAGCAAAAATGCGAGGTTATCGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGTTTACTTTCAAAGGAACTGGGAGATTTCTGCTTAAGTCAAATTTTGTCAGGAGGGTCATCTGAAGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTGCCCTCGAGAAATATATCATCACCAAGACATTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAGTGGTTACTCTACTGGTTGCTCTGTTGGAGATACAGTCCCTTATATTATTTGCTGTGAGCAGGGTACAAGTTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAGATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCTGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACCAGCCCCCAACGCTTGGCTGATTGTTTAGGGATTGACTCATCCAAGTTCAAATCGAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTGTTGACGATGAAGAGAGATATCGGGATTGCGAGTCTTTGATCTTAGCATGTCCTAGCTGCTCTGGTACTTTTGATTGTCCAGCCATCTTCAACTCCATTGCCAAGTCAATCAATGAAAAGCCAACAAGGCCACAAGGAGAAGAATCTTCTAGCGATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCCAATCAGGTCAAAAGGCAAGCAGAGGGTTTTGTTTCAATGTACTACAGGGGCATAATGACGTGTGATGATGAAACTTGCAAATACACCACGCGAAGTCTCAACCTCCGGCTTGTTGGTGATGCTGAGAGAGGAACAGTCTGTCCAAACTATCCTCACTGCAATGGGCATCTTGTAAGAAAGTACACGGAAGCTGATTTGTACAAACAGCTCTCATTTTTCTGCCATGTCTTGGATACAGTACGCTGCGTAGAAAAGATGGAACCTAAGACTAGACTGCCGGTAGAGTTAGAGTTGGCCAAAATTCGCCCCATTGTGACTTTGGCTGCCTCAACAGTTGAAAGGATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTGCAAGATCTTTTTGTTACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1538

Amino Acids

171.8

Weight (kDa)

6.77

Isoelectric Point (pI)

46.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	27 - 96	2e-18	DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1	PF03104	509 - 763	1.5e-26	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	829 - 1291	4.5e-125	DNA polymerase family B
zf-DNA_Pol	PF08996	1329 - 1535	2.3e-43	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3098
AatII	GACGTC	2 cut(s)	246, 311
Acc36I	ACCTGC	1 cut(s)	1432
Acc65I	GGTACC	1 cut(s)	3878
AccB1I	GGYRCC	4 cut(s)	36, 814, 1381, 3878
AccB7I	CCANNNNNTGG	2 cut(s)	3895, 4444
AccI	GTMKAC	1 cut(s)	246
AccII	CGCG	4 cut(s)	67, 216, 956, 4306
AccIII	TCCGGA	1 cut(s)	1210
AclWI	GGATC	6 cut(s)	914, 927, 1362, 1463, 1476, 3144
AcoI	YGGCCR	2 cut(s)	109, 4509
AcsI	RAATTY	8 cut(s)	1594, 1637, 1936, 2857, 2926, 3465, 3937, 4515
AcuI	CTGAAG	5 cut(s)	1302, 1466, 2612, 3038, 3507
AcyI	GRCGYC	3 cut(s)	37, 243, 308
AdeI	CACNNNGTG	1 cut(s)	2696
AflII	CTTAAG	1 cut(s)	3457
AflIII	ACRYGT	3 cut(s)	2456, 3631, 4274
AgeI	ACCGGT	1 cut(s)	135
AjiI	CACGTC	3 cut(s)	459, 2459, 4275
AjnI	CCWGG	6 cut(s)	78, 341, 1849, 2197, 2408, 2899
AleI	CACNNNNGTG	1 cut(s)	1823
AloI	GAACNNNNNNTCC	2 cut(s)	4339, 4371
Alw21I	GWGCWC	4 cut(s)	97, 1513, 2089, 2446
Alw26I	GTCTC	6 cut(s)	1681, 1783, 2393, 2984, 4203, 4316
AlwI	GGATC	6 cut(s)	914, 927, 1362, 1463, 1476, 3144
Ama87I	CYCGRG	4 cut(s)	296, 818, 1270, 3560
Aor13HI	TCCGGA	1 cut(s)	1210
ApoI	RAATTY	8 cut(s)	1594, 1637, 1936, 2857, 2926, 3465, 3937, 4515
AsiGI	ACCGGT	1 cut(s)	135
Asp700I	GAANNNNTTC	3 cut(s)	3504, 3961, 4557
Asp718I	GGTACC	1 cut(s)	3878
AspA2I	CCTAGG	1 cut(s)	253
AspLEI	GCGC	9 cut(s)	39, 67, 77, 87, 213, 550, 1535, 3646, 4158
AsuC2I	CCSGG	4 cut(s)	973, 1466, 3113, 3748
AsuHPI	GGTGA	8 cut(s)	500, 880, 1272, 1657, 3188, 3568, 4193, 4343
AvaI	CYCGRG	4 cut(s)	296, 818, 1270, 3560
AvaII	GGWCC	7 cut(s)	69, 288, 1103, 1571, 2118, 2720, 4564
AvrII	CCTAGG	1 cut(s)	253
BaeGI	GKGCMC	4 cut(s)	586, 819, 1118, 2518
BalI	TGGCCA	1 cut(s)	4511
BamHI	GGATCC	2 cut(s)	919, 1468
BanI	GGYRCC	4 cut(s)	36, 814, 1381, 3878
BanII	GRGCYC	2 cut(s)	97, 2089
BarI	GAAGNNNNNNTAC	2 cut(s)	149, 181
BbsI	GAAGAC	1 cut(s)	836
Bbv12I	GWGCWC	4 cut(s)	97, 1513, 2089, 2446
BbvCI	CCTCAGC	1 cut(s)	846
BceAI	ACGGC	4 cut(s)	96, 265, 286, 426
BcgI	CGANNNNNNTGC	4 cut(s)	176, 210, 2387, 2421
BciT130I	CCWGG	6 cut(s)	80, 343, 1851, 2199, 2410, 2901
BciVI	GTATCC	3 cut(s)	2590, 4441, 4578
BcnI	CCSGG	4 cut(s)	973, 1466, 3113, 3748
BcoDI	GTCTC	6 cut(s)	1681, 1783, 2393, 2984, 4203, 4316
BfaI	CTAG	8 cut(s)	254, 1517, 2349, 2889, 3765, 4038, 4134, 4485
BfmI	CTRYAG	4 cut(s)	1425, 1698, 1926, 4258
BfoI	RGCGCY	4 cut(s)	40, 78, 88, 214
BfrI	CTTAAG	1 cut(s)	3457
BfuAI	ACCTGC	1 cut(s)	1432
BfuI	GTATCC	3 cut(s)	2590, 4441, 4578
BglI	GCCNNNNNGGC	1 cut(s)	4111
BglII	AGATCT	3 cut(s)	1486, 3117, 4597
BlnI	CCTAGG	1 cut(s)	253
BlpI	GCTNAGC	1 cut(s)	1859
BmcAI	AGTACT	1 cut(s)	3130
Bme18I	GGWCC	7 cut(s)	69, 288, 1103, 1571, 2118, 2720, 4564
BmeT110I	CYCGRG	4 cut(s)	296, 818, 1270, 3560
BmgBI	CACGTC	3 cut(s)	459, 2459, 4275
BmrI	ACTGGG	1 cut(s)	3452
BmuI	ACTGGG	1 cut(s)	3452
BpiI	GAAGAC	1 cut(s)	836
BpmI	CTGGAG	3 cut(s)	101, 2208, 3442
Bpu10I	CCTNAGC	3 cut(s)	846, 2088, 2154
Bpu1102I	GCTNAGC	1 cut(s)	1859
BpuEI	CTTGAG	6 cut(s)	1695, 1981, 2120, 2966, 3049, 3308
BpuMI	CCSGG	4 cut(s)	973, 1466, 3113, 3748
BsaBI	GATNNNNATC	2 cut(s)	918, 1041
BsaHI	GRCGYC	3 cut(s)	37, 243, 308
BsaI	GGTCTC	2 cut(s)	1681, 2984
BsaWI	WCCGGW	3 cut(s)	135, 1210, 2319
BsaXI	ACNNNNNCTCC	2 cut(s)	3681, 3711
Bse118I	RCCGGY	4 cut(s)	135, 589, 614, 4492
Bse1I	ACTGG	7 cut(s)	290, 967, 985, 3090, 3425, 3447, 3679
Bse3DI	GCAATG	4 cut(s)	1000, 2214, 4080, 4381
Bse8I	GATNNNNATC	2 cut(s)	918, 1041
BseAI	TCCGGA	1 cut(s)	1210
BseBI	CCWGG	6 cut(s)	80, 343, 1851, 2199, 2410, 2901
BseJI	GATNNNNATC	2 cut(s)	918, 1041
BseMI	GCAATG	4 cut(s)	1000, 2214, 4080, 4381
BseNI	ACTGG	7 cut(s)	290, 967, 985, 3090, 3425, 3447, 3679
BseRI	GAGGAG	6 cut(s)	63, 233, 361, 592, 836, 2956
BseSI	GKGCMC	4 cut(s)	586, 819, 1118, 2518
BseX3I	CGGCCG	1 cut(s)	109
BseYI	CCCAGC	2 cut(s)	1609, 2308
BsgI	GTGCAG	2 cut(s)	872, 4168
Bsh1236I	CGCG	4 cut(s)	67, 216, 956, 4306
Bsh1285I	CGRYCG	4 cut(s)	24, 112, 136, 177
BshNI	GGYRCC	4 cut(s)	36, 814, 1381, 3878
BshTI	ACCGGT	1 cut(s)	135
BsiEI	CGRYCG	4 cut(s)	24, 112, 136, 177
BsiHKAI	GWGCWC	4 cut(s)	97, 1513, 2089, 2446
BsiHKCI	CYCGRG	4 cut(s)	296, 818, 1270, 3560
BslFI	GGGAC	4 cut(s)	2436, 3683, 3814, 4577
BsmAI	GTCTC	6 cut(s)	1681, 1783, 2393, 2984, 4203, 4316
BsmFI	GGGAC	4 cut(s)	2436, 3683, 3814, 4577
BsmI	GAATGC	4 cut(s)	901, 1660, 2974, 3318
Bso31I	GGTCTC	2 cut(s)	1681, 2984
BsoBI	CYCGRG	4 cut(s)	296, 818, 1270, 3560
Bsp1286I	GDGCHC	8 cut(s)	97, 586, 819, 1118, 1513, 2089, 2446, 2518
Bsp13I	TCCGGA	1 cut(s)	1210
Bsp1407I	TGTACA	2 cut(s)	3303, 4413
Bsp1720I	GCTNAGC	1 cut(s)	1859
BspEI	TCCGGA	1 cut(s)	1210
BspFNI	CGCG	4 cut(s)	67, 216, 956, 4306
BspMAI	CTGCAG	2 cut(s)	1429, 1930
BspMI	ACCTGC	1 cut(s)	1432
BspPI	GGATC	6 cut(s)	914, 927, 1362, 1463, 1476, 3144
BspQI	GCTCTTC	2 cut(s)	1518, 2451
BspT107I	GGYRCC	4 cut(s)	36, 814, 1381, 3878
BspTI	CTTAAG	1 cut(s)	3457
BspTNI	GGTCTC	2 cut(s)	1681, 2984
BsrDI	GCAATG	4 cut(s)	1000, 2214, 4080, 4381
BsrFI	RCCGGY	4 cut(s)	135, 589, 614, 4492
BsrGI	TGTACA	2 cut(s)	3303, 4413
BsrI	ACTGG	7 cut(s)	290, 967, 985, 3090, 3425, 3447, 3679
BssAI	RCCGGY	4 cut(s)	135, 589, 614, 4492
BssNI	GRCGYC	3 cut(s)	37, 243, 308
BssT1I	CCWWGG	2 cut(s)	253, 2503
Bst2UI	CCWGG	6 cut(s)	80, 343, 1851, 2199, 2410, 2901
Bst6I	CTCTTC	8 cut(s)	273, 617, 873, 1086, 1518, 1838, 2451, 3990
BstACI	GRCGYC	3 cut(s)	37, 243, 308
BstAFI	CTTAAG	1 cut(s)	3457
BstAPI	GCANNNNNTGC	1 cut(s)	932
BstAUI	TGTACA	2 cut(s)	3303, 4413
BstC8I	GCNNGC	6 cut(s)	591, 855, 2160, 2544, 3012, 4233
BstFNI	CGCG	4 cut(s)	67, 216, 956, 4306
BstH2I	RGCGCY	4 cut(s)	40, 78, 88, 214
BstHHI	GCGC	9 cut(s)	39, 67, 77, 87, 213, 550, 1535, 3646, 4158
BstMAI	GTCTC	6 cut(s)	1681, 1783, 2393, 2984, 4203, 4316
BstMCI	CGRYCG	4 cut(s)	24, 112, 136, 177
BstNI	CCWGG	6 cut(s)	80, 343, 1851, 2199, 2410, 2901
BstNSI	RCATGY	6 cut(s)	1078, 1189, 2116, 2546, 3635, 4035
BstSFI	CTRYAG	4 cut(s)	1425, 1698, 1926, 4258
BstSLI	GKGCMC	4 cut(s)	586, 819, 1118, 2518
BstUI	CGCG	4 cut(s)	67, 216, 956, 4306
BstV2I	GAAGAC	1 cut(s)	836
BstX2I	RGATCY	7 cut(s)	919, 1354, 1468, 1486, 3117, 3136, 4597
BstXI	CCANNNNNNTGG	1 cut(s)	79
BstYI	RGATCY	7 cut(s)	919, 1354, 1468, 1486, 3117, 3136, 4597
BstZI	CGGCCG	1 cut(s)	109
BsuI	GTATCC	3 cut(s)	2590, 4441, 4578
BtgZI	GCGATG	3 cut(s)	187, 437, 971
BtrI	CACGTC	3 cut(s)	459, 2459, 4275
BtsI	GCAGTG	3 cut(s)	1061, 1981, 4369
BtsIMutI	CAGTG	5 cut(s)	1061, 1116, 1981, 3083, 4369
BveI	ACCTGC	1 cut(s)	1432
Cac8I	GCNNGC	6 cut(s)	591, 855, 2160, 2544, 3012, 4233
CfoI	GCGC	9 cut(s)	39, 67, 77, 87, 213, 550, 1535, 3646, 4158
Cfr10I	RCCGGY	4 cut(s)	135, 589, 614, 4492
CspAI	ACCGGT	1 cut(s)	135
DinI	GGCGCC	1 cut(s)	38
DraIII	CACNNNGTG	1 cut(s)	2696
EaeI	YGGCCR	2 cut(s)	109, 4509
EagI	CGGCCG	1 cut(s)	109
Eam1104I	CTCTTC	8 cut(s)	273, 617, 873, 1086, 1518, 1838, 2451, 3990
EarI	CTCTTC	8 cut(s)	273, 617, 873, 1086, 1518, 1838, 2451, 3990
EciI	GGCGGA	2 cut(s)	20, 872
Ecl136II	GAGCTC	2 cut(s)	95, 2087
EclXI	CGGCCG	1 cut(s)	109
Eco130I	CCWWGG	2 cut(s)	253, 2503
Eco24I	GRGCYC	2 cut(s)	97, 2089
Eco31I	GGTCTC	2 cut(s)	1681, 2984
Eco32I	GATATC	1 cut(s)	4004
Eco47I	GGWCC	7 cut(s)	69, 288, 1103, 1571, 2118, 2720, 4564
Eco52I	CGGCCG	1 cut(s)	109
Eco53kI	GAGCTC	2 cut(s)	95, 2087
Eco57I	CTGAAG	5 cut(s)	1302, 1466, 2612, 3038, 3507
Eco88I	CYCGRG	4 cut(s)	296, 818, 1270, 3560
EcoICRI	GAGCTC	2 cut(s)	95, 2087
EcoO109I	RGGNCCY	3 cut(s)	1571, 2720, 3397
EcoRI	GAATTC	2 cut(s)	1936, 3937
EcoRII	CCWGG	6 cut(s)	78, 341, 1849, 2197, 2408, 2899
EcoRV	GATATC	1 cut(s)	4004
EcoT14I	CCWWGG	2 cut(s)	253, 2503
EcoT22I	ATGCAT	1 cut(s)	2974
EcoT38I	GRGCYC	2 cut(s)	97, 2089
EgeI	GGCGCC	1 cut(s)	38
EheI	GGCGCC	1 cut(s)	38
ErhI	CCWWGG	2 cut(s)	253, 2503
FaqI	GGGAC	4 cut(s)	2436, 3683, 3814, 4577
FblI	GTMKAC	1 cut(s)	246
FriOI	GRGCYC	2 cut(s)	97, 2089
FspBI	CTAG	8 cut(s)	254, 1517, 2349, 2889, 3765, 4038, 4134, 4485
GlaI	GCGC	9 cut(s)	38, 66, 76, 86, 212, 549, 1534, 3645, 4157
GsaI	CCCAGC	2 cut(s)	1613, 2312
GsuI	CTGGAG	3 cut(s)	101, 2208, 3442
HaeII	RGCGCY	4 cut(s)	40, 78, 88, 214
HhaI	GCGC	9 cut(s)	39, 67, 77, 87, 213, 550, 1535, 3646, 4158
Hin1I	GRCGYC	3 cut(s)	37, 243, 308
Hin6I	GCGC	9 cut(s)	37, 65, 75, 85, 211, 548, 1533, 3644, 4156
HinP1I	GCGC	9 cut(s)	37, 65, 75, 85, 211, 548, 1533, 3644, 4156
HincII	GTYRAC	4 cut(s)	247, 1768, 3262, 3988
HindII	GTYRAC	4 cut(s)	247, 1768, 3262, 3988
HindIII	AAGCTT	3 cut(s)	1247, 1883, 2156
HpaI	GTTAAC	2 cut(s)	1768, 3262
HphI	GGTGA	8 cut(s)	500, 880, 1272, 1657, 3188, 3568, 4193, 4343
Hpy166II	GTNNAC	8 cut(s)	247, 456, 1768, 1914, 3262, 3428, 3988, 4398
Hpy8I	GTNNAC	8 cut(s)	247, 456, 1768, 1914, 3262, 3428, 3988, 4398
HpyCH4IV	ACGT	7 cut(s)	243, 308, 458, 2458, 3412, 3760, 4274
HpySE526I	ACGT	7 cut(s)	243, 308, 458, 2458, 3412, 3760, 4274
Hsp92I	GRCGYC	3 cut(s)	37, 243, 308
HspAI	GCGC	9 cut(s)	37, 65, 75, 85, 211, 548, 1533, 3644, 4156
KasI	GGCGCC	1 cut(s)	36
Kpn2I	TCCGGA	1 cut(s)	1210
KpnI	GGTACC	1 cut(s)	3882
KroI	GCCGGC	1 cut(s)	589
KroNI	GCCGGC	1 cut(s)	591
KspAI	GTTAAC	2 cut(s)	1768, 3262
LguI	GCTCTTC	2 cut(s)	1518, 2451
LmnI	GCTCC	9 cut(s)	42, 50, 82, 859, 1618, 1663, 2189, 2749, 3352
MaeI	CTAG	8 cut(s)	254, 1517, 2349, 2889, 3765, 4038, 4134, 4485
MaeII	ACGT	7 cut(s)	243, 308, 458, 2458, 3412, 3760, 4274
MfeI	CAATTG	1 cut(s)	3234
MflI	RGATCY	7 cut(s)	919, 1354, 1468, 1486, 3117, 3136, 4597
MhlI	GDGCHC	8 cut(s)	97, 586, 819, 1118, 1513, 2089, 2446, 2518
MlsI	TGGCCA	1 cut(s)	4511
MluNI	TGGCCA	1 cut(s)	4511
Mly113I	GGCGCC	1 cut(s)	37
MlyI	GAGTC	9 cut(s)	1007, 1262, 1826, 1855, 2296, 2418, 3179, 3911, 4025
MmeI	TCCRAC	6 cut(s)	732, 1332, 2001, 2020, 2637, 3667
Mox20I	TGGCCA	1 cut(s)	4511
Mph1103I	ATGCAT	1 cut(s)	2974
MroI	TCCGGA	1 cut(s)	1210
MroNI	GCCGGC	1 cut(s)	589
MroXI	GAANNNNTTC	3 cut(s)	3504, 3961, 4557
MscI	TGGCCA	1 cut(s)	4511
MslI	CAYNNNNRTG	5 cut(s)	1823, 2245, 2439, 3774, 4374
Msp20I	TGGCCA	1 cut(s)	4511
MspA1I	CMGCKG	3 cut(s)	2308, 2464, 4591
MspCI	CTTAAG	1 cut(s)	3457
MunI	CAATTG	1 cut(s)	3234
Mva1269I	GAATGC	4 cut(s)	901, 1660, 2974, 3318
MvaI	CCWGG	6 cut(s)	80, 343, 1851, 2199, 2410, 2901
MvnI	CGCG	4 cut(s)	67, 216, 956, 4306
NaeI	GCCGGC	1 cut(s)	591
NarI	GGCGCC	1 cut(s)	37
NciI	CCSGG	4 cut(s)	973, 1466, 3113, 3748
NgoMIV	GCCGGC	1 cut(s)	589
NmeAIII	GCCGAG	4 cut(s)	71, 79, 565, 1288
NmuCI	GTSAC	6 cut(s)	25, 1010, 1059, 2255, 3416, 4528
NsiI	ATGCAT	1 cut(s)	2974
NspI	RCATGY	6 cut(s)	1078, 1189, 2116, 2546, 3635, 4035
OliI	CACNNNNGTG	1 cut(s)	1823
PaeI	GCATGC	1 cut(s)	2546
PaeR7I	CTCGAG	2 cut(s)	1270, 3560
PciI	ACATGT	1 cut(s)	3631
PciSI	GCTCTTC	2 cut(s)	1518, 2451
PcsI	WCGNNNNNNNCGW	3 cut(s)	174, 183, 455
PctI	GAATGC	4 cut(s)	901, 1660, 2974, 3318
PdiI	GCCGGC	1 cut(s)	591
PdmI	GAANNNNTTC	3 cut(s)	3504, 3961, 4557
PflFI	GACNNNGTC	1 cut(s)	173
PflMI	CCANNNNNTGG	2 cut(s)	3895, 4444
PfoI	TCCNGGA	1 cut(s)	1464
PinAI	ACCGGT	1 cut(s)	135
Ple19I	CGATCG	1 cut(s)	24
PleI	GAGTC	9 cut(s)	1007, 1262, 1826, 1854, 2296, 2418, 3179, 3911, 4024
PluTI	GGCGCC	1 cut(s)	40
PpsI	GAGTC	9 cut(s)	1007, 1262, 1826, 1854, 2296, 2418, 3179, 3911, 4024
PpuMI	RGGWCCY	2 cut(s)	1571, 2720
PscI	ACATGT	1 cut(s)	3631
PsiI	TTATAA	1 cut(s)	3098
Psp124BI	GAGCTC	2 cut(s)	97, 2089
Psp5II	RGGWCCY	2 cut(s)	1571, 2720
Psp6I	CCWGG	6 cut(s)	78, 341, 1849, 2197, 2408, 2899
PspFI	CCCAGC	2 cut(s)	1609, 2308
PspGI	CCWGG	6 cut(s)	78, 341, 1849, 2197, 2408, 2899
PspPPI	RGGWCCY	2 cut(s)	1571, 2720
PspXI	VCTCGAGB	1 cut(s)	1270
PstI	CTGCAG	2 cut(s)	1429, 1930
PsuI	RGATCY	7 cut(s)	919, 1354, 1468, 1486, 3117, 3136, 4597
PsyI	GACNNNGTC	1 cut(s)	173
PvuI	CGATCG	1 cut(s)	24
PvuII	CAGCTG	3 cut(s)	2308, 2464, 4591
RseI	CAYNNNNRTG	5 cut(s)	1823, 2245, 2439, 3774, 4374
SacI	GAGCTC	2 cut(s)	97, 2089
SalI	GTCGAC	1 cut(s)	245
SapI	GCTCTTC	2 cut(s)	1518, 2451
SbfI	CCTGCAGG	1 cut(s)	1429
ScaI	AGTACT	1 cut(s)	3130
SchI	GAGTC	9 cut(s)	1007, 1262, 1826, 1855, 2296, 2418, 3179, 3911, 4025
SdaI	CCTGCAGG	1 cut(s)	1429
SduI	GDGCHC	8 cut(s)	97, 586, 819, 1118, 1513, 2089, 2446, 2518
SfcI	CTRYAG	4 cut(s)	1425, 1698, 1926, 4258
SfoI	GGCGCC	1 cut(s)	38
Sfr274I	CTCGAG	2 cut(s)	1270, 3560
SgrDI	CGTCGACG	1 cut(s)	245
SinI	GGWCC	7 cut(s)	69, 288, 1103, 1571, 2118, 2720, 4564
SlaI	CTCGAG	2 cut(s)	1270, 3560
SmiMI	CAYNNNNRTG	5 cut(s)	1823, 2245, 2439, 3774, 4374
SmlI	CTYRAG	9 cut(s)	1270, 1710, 1960, 2135, 2981, 3064, 3323, 3457, 3560
SmoI	CTYRAG	9 cut(s)	1270, 1710, 1960, 2135, 2981, 3064, 3323, 3457, 3560
SphI	GCATGC	1 cut(s)	2546
Sse8387I	CCTGCAGG	1 cut(s)	1429
SspDI	GGCGCC	1 cut(s)	36
SspMI	CTAG	8 cut(s)	254, 1517, 2349, 2889, 3765, 4038, 4134, 4485
SstI	GAGCTC	2 cut(s)	97, 2089
StyI	CCWWGG	2 cut(s)	253, 2503
TaiI	ACGT	7 cut(s)	246, 311, 461, 2461, 3415, 3763, 4277
TaqI	TCGA	9 cut(s)	177, 225, 246, 305, 449, 1271, 3387, 3561, 3935
TatI	WGTACW	6 cut(s)	1441, 3128, 3303, 4254, 4395, 4413
TscAI	CASTG	5 cut(s)	1068, 1123, 1981, 3090, 4376
TseFI	GTSAC	6 cut(s)	25, 1010, 1059, 2255, 3416, 4528
Tsp45I	GTSAC	6 cut(s)	25, 1010, 1059, 2255, 3416, 4528
TspGWI	ACGGA	5 cut(s)	589, 1868, 2629, 3197, 4415
TspRI	CASTG	5 cut(s)	1068, 1123, 1981, 3090, 4376
Tth111I	GACNNNGTC	1 cut(s)	173
Van91I	CCANNNNNTGG	2 cut(s)	3895, 4444
Vha464I	CTTAAG	1 cut(s)	3457
VpaK11BI	GGWCC	7 cut(s)	69, 288, 1103, 1571, 2118, 2720, 4564
XapI	RAATTY	8 cut(s)	1594, 1637, 1936, 2857, 2926, 3465, 3937, 4515
XceI	RCATGY	6 cut(s)	1078, 1189, 2116, 2546, 3635, 4035
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2112, 4531
XhoI	CTCGAG	2 cut(s)	1270, 3560
XmaJI	CCTAGG	1 cut(s)	253
XmiI	GTMKAC	1 cut(s)	246
XmnI	GAANNNNTTC	3 cut(s)	3504, 3961, 4557
XspI	CTAG	8 cut(s)	254, 1517, 2349, 2889, 3765, 4038, 4134, 4485
ZraI	GACGTC	2 cut(s)	244, 309
ZrmI	AGTACT	1 cut(s)	3130
Zsp2I	ATGCAT	1 cut(s)	2974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.