Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Reverse (-)

28732867 .. 28742949

Gene Structure 10083 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G020950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4623 bp

ATGGCGGACGACGAGCAACCGATCGTGACCGGGAGGCGCCGGAGCCGAGGAGCCGAGGCGTCGGCGCGGTCCAGCGCCCTGGAGCGCCTCCGAGCTCTCCGGCAAGGCGGCCGTCGCTCTGAAAACGCCGACGACCGGTTCCAGATCAAGATGGAGAAGCCGATCTACGACACTGTTGACGAGGACGAGTACGACGCCATCATCGCCAAGCGGCGCGAGGAGTTCGGGGACTTCATCGAGGACGACGACGGCCTAGGGTACCGCGACGACGGCGAGGAAGAGGACTGGACCCGCCGCGGGCTTCCGACGTCGTCGGATGAATCGGACGGCGATATGAGGCCCAGGAGGAGGAAGAAGGTGGAGAAGAAGGAGAAGGAGAAGGAGCCGCGGCCCAAAAAGCCCAATTCGTCGCTTACGGCGGCGGCGGCGATGATGGGGAAGCAGAGGCTTTCGTCGATGTTCACGTCGTCGGTGTTTAATAAAAGTAGGGATGGTGACAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCCGAGTTTGCGCCAGACGAGGCTGATAGAGAGAAACGGAGGAGGGCACAGCCGGCAAGGAGTTTCGTTCCGATTGCCGGTGTGAAGAGTGAGAGAGTGAGTAATGTGGGTGTTGAGTTATCAAGGAGGCTTGAATTGGATAGAATTGTTGCAAATGGTGGTGGTTCTGTGCATAGTAAGGAAATGGATAAAGATTCTGAGGGCAAGAAGGAGTTGGATTTCGGAAATTGTCAGCCCCAAATTGAGCAATTCCATGATTTTTTGGAGAATAGTGAGGTGCCCGAGGAGAAGAAGACTGAGGATTCTGCTGAGGTGCAGGCGGAGCCTGTGGTGAAAAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATGAGGATCCGGCATTGAGTGCTATGGCAGGATGGAAGGCCGCGATGACTGGTGGGGCCGGGGTTACTGGTGGCGGCGGCAATGTGGAGGTCAAAAGTGACTCAATTAGTGAAGGGAGTTCGGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAATATGGGCACTGTTTATCTATTTGGGAAGGTTAAAGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTAAAGAACATGCAAAGATGTGTCTATGCAATTCCGGACAGTCCTTTATTTCAAACTGACGAAATGATGAAGCTTGAAAAGGATGCCGAGGACTCGAGGATTTCACCCACCGATTTTCGTAAGAAGCTGCATGATGCGGCTTCAGAATTAAAGAATGAATTAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGGCACCTGTCAAGAGGAACTATGCTTTTGAGCGATCTGACATACCTGCAGGGGAGAATTATGTACTGAAGATCAATTATCCATTCCGGGATCCACCACTATCAGCAGATCTAAAAGGAGAAACTTTTTGTGCTCTTCTAGGAACTCATAGCAGCGCATTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAAATTTTTCCATCTGCCCAGCTCCACAACGGGTAAGCTGGTGCAAATTTGAGGTGATCGTGAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACAAAGAAAACTGTAGATATTCCTCAAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTATCAATGAGAAGCAGAATGTTAACGAAATAGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCCGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGTGGCATATTTCCTATGGGGTTTACTAAAGTGGCTGCAGATAAGAATTCTAAAGCCGGAACAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTAAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTTGTTGGACATAATATATCTGGATTTGATCTGGATGTTCTTCTCCACAGAGCTCAGGTTTGCAGAGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATCATGTCCTGCATTGCTGGTCGGCTGTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAAGGAGGTCAGCTATTCCTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCACATGATATTCCTAGAATGTTACAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCCTGGTTGTCTATGGGACTCATGTTTCATTTGAGTGCTCTTCCCCTCACACGTCAGCTGACTAATATAAGTGGCAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTAGAATATCTCTTACTGCATGCTTTTCATGCAAAAAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGTGTTGAGGAAAAAGCTGCTGATGAGTTGGATGTAAATGATGTAAATGTTGATAATGATGCACAGAGTGGGCATGGAAAAAGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTATTGGAGCCAAAAAAAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATATGCTTCACAACTGTTGAAAGAAATTCAGAAGGATTGGTTTCTCATTTACCTTCTAGTCAAAGACCTGGACTTTTACCTGAGTTGTTAAAAAATTTAGTTGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAACTTGACATTCAACAGCAAGCTCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAACTCAAGATTTTATGCAAAGCCACTGGCAGAGCTTATAACACTACGGGGCCGGGAGATCTTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTAATCTATGGTGATACGGACTCAATAATGATTTATAGTGGGCTGGATGATAGTGTGAAAGCAAAAGCAATTGCTGTGAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGACGGTTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCGGCTGTGAAGGAGCTAATGAAAAACGAGCAAAAATGCGAGGTTATCGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGTTTACTTTCAAAGGAACTGGGAGATTTCTGCTTAAGTCAAATTTTGTCAGGAGGGTCATCTGAAGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTGCCCTCGAGAAATATATCATCACCAAGACATTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAGTGGTTACTCTACTGGTTGCTCTGTTGGAGATACAGTCCCTTATATTATTTGCCGTGAGCAGGGCACAAGTTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAGATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCTGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACCAGCCCCCAACGCTTGGCTGATTGTTTAGGGATTGACTCATCCAAGTTCAAATCGAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTGTTGACGATGAAGAGAGATATCGGGATTGCGAGTCTTTGATCTTAGCATGTCCTAGCTGCTCTGGTACTTTTGATTGTCCAGCCATCTTCAACTCCATTGCCAAGTCAATCAATGAAAAGCCAACAAGGCCACAAGGAGAAGAATCTTCTAGCGATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCCAATCAGGTCAAAAGGCAAGCAGAGGGTTTTGTTTCAATGTACTACAGGGGCATAATGACGTGTGATGATGAAACTTGCAAATACACCACACGAAGTCTCAACCTCCGGCTTGTTGGTGATGCTGAGAGAGGAACAGTCTGTCCAAACTATCCTCACTGCAATGGGCATCTTGTAAGAAAGTACACGGAAGCTGATTTGTACAGACAGCTCTCATTTTTCTGCCATGTCTTGGATACAGTACGCTGCGTAGAAAAGATGGAACCTAAGACTAGACTGCCGGTAGAGTTAGAGTTGGCCAAAATTCGCCCCATTGTGACTTTGGCTGCCTCAACAGTTGAAAGGATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTGCAAGATCTTTTTGTTACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1540

Amino Acids

172.27

Weight (kDa)

7.38

Isoelectric Point (pI)

46.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	27 - 96	9.6e-20	DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1	PF03104	511 - 765	1.5e-26	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	831 - 1293	1.6e-124	DNA polymerase family B
zf-DNA_Pol	PF08996	1331 - 1537	3.7e-43	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3104
AatII	GACGTC	1 cut(s)	311
Acc36I	ACCTGC	1 cut(s)	1438
Acc65I	GGTACC	2 cut(s)	258, 3884
AccB1I	GGYRCC	5 cut(s)	36, 258, 820, 1387, 3884
AccB7I	CCANNNNNTGG	2 cut(s)	3901, 4450
AccII	CGCG	6 cut(s)	67, 216, 264, 297, 388, 962
AccIII	TCCGGA	1 cut(s)	1216
AclWI	GGATC	7 cut(s)	920, 933, 1368, 1469, 1482, 2216, 3150
AcoI	YGGCCR	2 cut(s)	109, 4515
AcsI	RAATTY	8 cut(s)	1600, 1643, 1942, 2863, 2932, 3471, 3943, 4521
AcuI	CTGAAG	5 cut(s)	1308, 1472, 2618, 3044, 3513
AcyI	GRCGYC	4 cut(s)	37, 59, 195, 308
AdeI	CACNNNGTG	1 cut(s)	2702
AflII	CTTAAG	1 cut(s)	3463
AflIII	ACRYGT	3 cut(s)	2462, 3637, 4280
AgeI	ACCGGT	1 cut(s)	135
AjiI	CACGTC	3 cut(s)	465, 2465, 4281
AjnI	CCWGG	6 cut(s)	78, 341, 1855, 2203, 2414, 2905
AleI	CACNNNNGTG	1 cut(s)	1829
AloI	GAACNNNNNNTCC	2 cut(s)	4345, 4377
Alw21I	GWGCWC	4 cut(s)	97, 1519, 2095, 2452
Alw26I	GTCTC	6 cut(s)	1687, 1789, 2399, 2990, 4209, 4322
AlwI	GGATC	7 cut(s)	920, 933, 1368, 1469, 1482, 2216, 3150
Ama87I	CYCGRG	3 cut(s)	824, 1276, 3566
Aor13HI	TCCGGA	1 cut(s)	1216
ApoI	RAATTY	8 cut(s)	1600, 1643, 1942, 2863, 2932, 3471, 3943, 4521
AsiGI	ACCGGT	1 cut(s)	135
Asp700I	GAANNNNTTC	3 cut(s)	3510, 3967, 4563
Asp718I	GGTACC	2 cut(s)	258, 3884
AspA2I	CCTAGG	1 cut(s)	253
AspLEI	GCGC	9 cut(s)	39, 67, 77, 87, 216, 556, 1541, 3652, 4164
AsuC2I	CCSGG	5 cut(s)	31, 979, 1472, 3119, 3754
AsuHPI	GGTGA	8 cut(s)	506, 886, 1278, 1663, 3194, 3574, 4199, 4349
AvaI	CYCGRG	3 cut(s)	824, 1276, 3566
AvaII	GGWCC	7 cut(s)	69, 288, 1109, 1577, 2124, 2726, 4570
AvrII	CCTAGG	1 cut(s)	253
BaeGI	GKGCMC	5 cut(s)	592, 825, 1124, 2524, 3734
BalI	TGGCCA	1 cut(s)	4517
BamHI	GGATCC	2 cut(s)	925, 1474
BanI	GGYRCC	5 cut(s)	36, 258, 820, 1387, 3884
BanII	GRGCYC	2 cut(s)	97, 2095
BarI	GAAGNNNNNNTAC	2 cut(s)	149, 181
BbsI	GAAGAC	1 cut(s)	842
Bbv12I	GWGCWC	4 cut(s)	97, 1519, 2095, 2452
BbvCI	CCTCAGC	1 cut(s)	852
BceAI	ACGGC	6 cut(s)	96, 265, 286, 343, 432, 3705
BcgI	CGANNNNNNTGC	2 cut(s)	2393, 2427
BciT130I	CCWGG	6 cut(s)	80, 343, 1857, 2205, 2416, 2907
BciVI	GTATCC	3 cut(s)	2596, 4447, 4584
BcnI	CCSGG	5 cut(s)	31, 979, 1472, 3119, 3754
BcoDI	GTCTC	6 cut(s)	1687, 1789, 2399, 2990, 4209, 4322
BfaI	CTAG	8 cut(s)	254, 1523, 2355, 2895, 3771, 4044, 4140, 4491
BfmI	CTRYAG	4 cut(s)	1431, 1704, 1932, 4264
BfoI	RGCGCY	3 cut(s)	40, 78, 88
BfrI	CTTAAG	1 cut(s)	3463
BfuAI	ACCTGC	1 cut(s)	1438
BfuI	GTATCC	3 cut(s)	2596, 4447, 4584
BglI	GCCNNNNNGGC	1 cut(s)	4117
BglII	AGATCT	3 cut(s)	1492, 3123, 4603
BlnI	CCTAGG	1 cut(s)	253
BlpI	GCTNAGC	1 cut(s)	1865
BmcAI	AGTACT	1 cut(s)	3136
Bme18I	GGWCC	7 cut(s)	69, 288, 1109, 1577, 2124, 2726, 4570
BmeT110I	CYCGRG	3 cut(s)	824, 1276, 3566
BmgBI	CACGTC	3 cut(s)	465, 2465, 4281
BmrI	ACTGGG	1 cut(s)	3458
BmuI	ACTGGG	1 cut(s)	3458
BpiI	GAAGAC	1 cut(s)	842
BpmI	CTGGAG	3 cut(s)	101, 2214, 3448
Bpu10I	CCTNAGC	3 cut(s)	852, 2094, 2160
Bpu1102I	GCTNAGC	1 cut(s)	1865
BpuEI	CTTGAG	6 cut(s)	1701, 1987, 2126, 2972, 3055, 3314
BpuMI	CCSGG	5 cut(s)	31, 979, 1472, 3119, 3754
BsaBI	GATNNNNATC	2 cut(s)	924, 1047
BsaHI	GRCGYC	4 cut(s)	37, 59, 195, 308
BsaI	GGTCTC	2 cut(s)	1687, 2990
BsaWI	WCCGGW	3 cut(s)	135, 1216, 2325
BsaXI	ACNNNNNCTCC	2 cut(s)	3687, 3717
Bse118I	RCCGGY	4 cut(s)	135, 595, 620, 4498
Bse1I	ACTGG	7 cut(s)	290, 973, 991, 3096, 3431, 3453, 3685
Bse3DI	GCAATG	4 cut(s)	1006, 2220, 4086, 4387
Bse8I	GATNNNNATC	2 cut(s)	924, 1047
BseAI	TCCGGA	1 cut(s)	1216
BseBI	CCWGG	6 cut(s)	80, 343, 1857, 2205, 2416, 2907
BseJI	GATNNNNATC	2 cut(s)	924, 1047
BseMI	GCAATG	4 cut(s)	1006, 2220, 4086, 4387
BseNI	ACTGG	7 cut(s)	290, 973, 991, 3096, 3431, 3453, 3685
BseRI	GAGGAG	6 cut(s)	63, 233, 361, 598, 842, 2962
BseSI	GKGCMC	5 cut(s)	592, 825, 1124, 2524, 3734
BseX3I	CGGCCG	1 cut(s)	109
BseYI	CCCAGC	2 cut(s)	1615, 2314
BsgI	GTGCAG	2 cut(s)	878, 4174
Bsh1236I	CGCG	6 cut(s)	67, 216, 264, 297, 388, 962
Bsh1285I	CGRYCG	3 cut(s)	24, 112, 136
BshNI	GGYRCC	5 cut(s)	36, 258, 820, 1387, 3884
BshTI	ACCGGT	1 cut(s)	135
BsiEI	CGRYCG	3 cut(s)	24, 112, 136
BsiHKAI	GWGCWC	4 cut(s)	97, 1519, 2095, 2452
BsiHKCI	CYCGRG	3 cut(s)	824, 1276, 3566
BslFI	GGGAC	5 cut(s)	242, 2442, 3689, 3820, 4583
BsmAI	GTCTC	6 cut(s)	1687, 1789, 2399, 2990, 4209, 4322
BsmFI	GGGAC	5 cut(s)	242, 2442, 3689, 3820, 4583
BsmI	GAATGC	4 cut(s)	907, 1666, 2980, 3324
Bso31I	GGTCTC	2 cut(s)	1687, 2990
BsoBI	CYCGRG	3 cut(s)	824, 1276, 3566
Bsp1286I	GDGCHC	9 cut(s)	97, 592, 825, 1124, 1519, 2095, 2452, 2524, 3734
Bsp13I	TCCGGA	1 cut(s)	1216
Bsp1407I	TGTACA	2 cut(s)	3309, 4419
Bsp1720I	GCTNAGC	1 cut(s)	1865
BspEI	TCCGGA	1 cut(s)	1216
BspFNI	CGCG	6 cut(s)	67, 216, 264, 297, 388, 962
BspMAI	CTGCAG	2 cut(s)	1435, 1936
BspMI	ACCTGC	1 cut(s)	1438
BspPI	GGATC	7 cut(s)	920, 933, 1368, 1469, 1482, 2216, 3150
BspQI	GCTCTTC	2 cut(s)	1524, 2457
BspT107I	GGYRCC	5 cut(s)	36, 258, 820, 1387, 3884
BspTI	CTTAAG	1 cut(s)	3463
BspTNI	GGTCTC	2 cut(s)	1687, 2990
BsrDI	GCAATG	4 cut(s)	1006, 2220, 4086, 4387
BsrFI	RCCGGY	4 cut(s)	135, 595, 620, 4498
BsrGI	TGTACA	2 cut(s)	3309, 4419
BsrI	ACTGG	7 cut(s)	290, 973, 991, 3096, 3431, 3453, 3685
BssAI	RCCGGY	4 cut(s)	135, 595, 620, 4498
BssNI	GRCGYC	4 cut(s)	37, 59, 195, 308
BssT1I	CCWWGG	2 cut(s)	253, 2509
Bst2UI	CCWGG	6 cut(s)	80, 343, 1857, 2205, 2416, 2907
Bst6I	CTCTTC	8 cut(s)	273, 623, 879, 1092, 1524, 1844, 2457, 3996
BstACI	GRCGYC	4 cut(s)	37, 59, 195, 308
BstAFI	CTTAAG	1 cut(s)	3463
BstAPI	GCANNNNNTGC	1 cut(s)	938
BstAUI	TGTACA	2 cut(s)	3309, 4419
BstC8I	GCNNGC	7 cut(s)	299, 597, 861, 2166, 2550, 3018, 4239
BstDSI	CCRYGG	2 cut(s)	295, 386
BstFNI	CGCG	6 cut(s)	67, 216, 264, 297, 388, 962
BstH2I	RGCGCY	3 cut(s)	40, 78, 88
BstHHI	GCGC	9 cut(s)	39, 67, 77, 87, 216, 556, 1541, 3652, 4164
BstMAI	GTCTC	6 cut(s)	1687, 1789, 2399, 2990, 4209, 4322
BstMCI	CGRYCG	3 cut(s)	24, 112, 136
BstNI	CCWGG	6 cut(s)	80, 343, 1857, 2205, 2416, 2907
BstNSI	RCATGY	6 cut(s)	1084, 1195, 2122, 2552, 3641, 4041
BstSFI	CTRYAG	4 cut(s)	1431, 1704, 1932, 4264
BstSLI	GKGCMC	5 cut(s)	592, 825, 1124, 2524, 3734
BstUI	CGCG	6 cut(s)	67, 216, 264, 297, 388, 962
BstV2I	GAAGAC	1 cut(s)	842
BstX2I	RGATCY	7 cut(s)	925, 1360, 1474, 1492, 3123, 3142, 4603
BstXI	CCANNNNNNTGG	1 cut(s)	79
BstYI	RGATCY	7 cut(s)	925, 1360, 1474, 1492, 3123, 3142, 4603
BstZI	CGGCCG	1 cut(s)	109
BsuI	GTATCC	3 cut(s)	2596, 4447, 4584
BtgI	CCRYGG	2 cut(s)	295, 386
BtgZI	GCGATG	3 cut(s)	187, 443, 977
BtrI	CACGTC	3 cut(s)	465, 2465, 4281
BtsI	GCAGTG	3 cut(s)	1067, 1987, 4375
BtsIMutI	CAGTG	6 cut(s)	171, 1067, 1122, 1987, 3089, 4375
BveI	ACCTGC	1 cut(s)	1438
Cac8I	GCNNGC	7 cut(s)	299, 597, 861, 2166, 2550, 3018, 4239
CfoI	GCGC	9 cut(s)	39, 67, 77, 87, 216, 556, 1541, 3652, 4164
Cfr10I	RCCGGY	4 cut(s)	135, 595, 620, 4498
Cfr42I	CCGCGG	2 cut(s)	298, 389
CseI	GACGC	2 cut(s)	48, 203
CspAI	ACCGGT	1 cut(s)	135
DinI	GGCGCC	1 cut(s)	38
DraIII	CACNNNGTG	1 cut(s)	2702
EaeI	YGGCCR	2 cut(s)	109, 4515
EagI	CGGCCG	1 cut(s)	109
Eam1104I	CTCTTC	8 cut(s)	273, 623, 879, 1092, 1524, 1844, 2457, 3996
EarI	CTCTTC	8 cut(s)	273, 623, 879, 1092, 1524, 1844, 2457, 3996
EciI	GGCGGA	2 cut(s)	20, 878
Ecl136II	GAGCTC	2 cut(s)	95, 2093
EclXI	CGGCCG	1 cut(s)	109
Eco130I	CCWWGG	2 cut(s)	253, 2509
Eco24I	GRGCYC	2 cut(s)	97, 2095
Eco31I	GGTCTC	2 cut(s)	1687, 2990
Eco32I	GATATC	1 cut(s)	4010
Eco47I	GGWCC	7 cut(s)	69, 288, 1109, 1577, 2124, 2726, 4570
Eco52I	CGGCCG	1 cut(s)	109
Eco53kI	GAGCTC	2 cut(s)	95, 2093
Eco57I	CTGAAG	5 cut(s)	1308, 1472, 2618, 3044, 3513
Eco88I	CYCGRG	3 cut(s)	824, 1276, 3566
EcoICRI	GAGCTC	2 cut(s)	95, 2093
EcoO109I	RGGNCCY	3 cut(s)	1577, 2726, 3403
EcoRI	GAATTC	2 cut(s)	1942, 3943
EcoRII	CCWGG	6 cut(s)	78, 341, 1855, 2203, 2414, 2905
EcoRV	GATATC	1 cut(s)	4010
EcoT14I	CCWWGG	2 cut(s)	253, 2509
EcoT22I	ATGCAT	1 cut(s)	2980
EcoT38I	GRGCYC	2 cut(s)	97, 2095
EgeI	GGCGCC	1 cut(s)	38
EheI	GGCGCC	1 cut(s)	38
ErhI	CCWWGG	2 cut(s)	253, 2509
FaqI	GGGAC	5 cut(s)	242, 2442, 3689, 3820, 4583
FauI	CCCGC	2 cut(s)	290, 299
FriOI	GRGCYC	2 cut(s)	97, 2095
FspBI	CTAG	8 cut(s)	254, 1523, 2355, 2895, 3771, 4044, 4140, 4491
GlaI	GCGC	9 cut(s)	38, 66, 76, 86, 215, 555, 1540, 3651, 4163
GsaI	CCCAGC	2 cut(s)	1619, 2318
GsuI	CTGGAG	3 cut(s)	101, 2214, 3448
HaeII	RGCGCY	3 cut(s)	40, 78, 88
HgaI	GACGC	2 cut(s)	48, 203
HhaI	GCGC	9 cut(s)	39, 67, 77, 87, 216, 556, 1541, 3652, 4164
Hin1I	GRCGYC	4 cut(s)	37, 59, 195, 308
Hin6I	GCGC	9 cut(s)	37, 65, 75, 85, 214, 554, 1539, 3650, 4162
HinP1I	GCGC	9 cut(s)	37, 65, 75, 85, 214, 554, 1539, 3650, 4162
HincII	GTYRAC	4 cut(s)	178, 1774, 3268, 3994
HindII	GTYRAC	4 cut(s)	178, 1774, 3268, 3994
HindIII	AAGCTT	3 cut(s)	1253, 1889, 2162
HpaI	GTTAAC	2 cut(s)	1774, 3268
HphI	GGTGA	8 cut(s)	506, 886, 1278, 1663, 3194, 3574, 4199, 4349
Hpy166II	GTNNAC	8 cut(s)	178, 462, 1774, 1920, 3268, 3434, 3994, 4404
Hpy8I	GTNNAC	8 cut(s)	178, 462, 1774, 1920, 3268, 3434, 3994, 4404
HpyCH4IV	ACGT	6 cut(s)	308, 464, 2464, 3418, 3766, 4280
HpySE526I	ACGT	6 cut(s)	308, 464, 2464, 3418, 3766, 4280
Hsp92I	GRCGYC	4 cut(s)	37, 59, 195, 308
HspAI	GCGC	9 cut(s)	37, 65, 75, 85, 214, 554, 1539, 3650, 4162
KasI	GGCGCC	1 cut(s)	36
Kpn2I	TCCGGA	1 cut(s)	1216
KpnI	GGTACC	2 cut(s)	262, 3888
KroI	GCCGGC	1 cut(s)	595
KroNI	GCCGGC	1 cut(s)	597
KspAI	GTTAAC	2 cut(s)	1774, 3268
KspI	CCGCGG	2 cut(s)	298, 389
LguI	GCTCTTC	2 cut(s)	1524, 2457
MaeI	CTAG	8 cut(s)	254, 1523, 2355, 2895, 3771, 4044, 4140, 4491
MaeII	ACGT	6 cut(s)	308, 464, 2464, 3418, 3766, 4280
MfeI	CAATTG	1 cut(s)	3240
MflI	RGATCY	7 cut(s)	925, 1360, 1474, 1492, 3123, 3142, 4603
MhlI	GDGCHC	9 cut(s)	97, 592, 825, 1124, 1519, 2095, 2452, 2524, 3734
MlsI	TGGCCA	1 cut(s)	4517
MluNI	TGGCCA	1 cut(s)	4517
Mly113I	GGCGCC	1 cut(s)	37
MlyI	GAGTC	9 cut(s)	1013, 1268, 1832, 1861, 2302, 2424, 3185, 3917, 4031
MmeI	TCCRAC	8 cut(s)	294, 329, 738, 1338, 2007, 2026, 2643, 3673
Mox20I	TGGCCA	1 cut(s)	4517
Mph1103I	ATGCAT	1 cut(s)	2980
MroI	TCCGGA	1 cut(s)	1216
MroNI	GCCGGC	1 cut(s)	595
MroXI	GAANNNNTTC	3 cut(s)	3510, 3967, 4563
MscI	TGGCCA	1 cut(s)	4517
MslI	CAYNNNNRTG	5 cut(s)	1829, 2251, 2445, 3780, 4380
Msp20I	TGGCCA	1 cut(s)	4517
MspA1I	CMGCKG	5 cut(s)	297, 388, 2314, 2470, 4597
MspCI	CTTAAG	1 cut(s)	3463
MunI	CAATTG	1 cut(s)	3240
Mva1269I	GAATGC	4 cut(s)	907, 1666, 2980, 3324
MvaI	CCWGG	6 cut(s)	80, 343, 1857, 2205, 2416, 2907
MvnI	CGCG	6 cut(s)	67, 216, 264, 297, 388, 962
NaeI	GCCGGC	1 cut(s)	597
NarI	GGCGCC	1 cut(s)	37
NciI	CCSGG	5 cut(s)	31, 979, 1472, 3119, 3754
NgoMIV	GCCGGC	1 cut(s)	595
NmeAIII	GCCGAG	4 cut(s)	71, 79, 571, 1294
NmuCI	GTSAC	7 cut(s)	25, 494, 1016, 1065, 2261, 3422, 4534
NsiI	ATGCAT	1 cut(s)	2980
NspI	RCATGY	6 cut(s)	1084, 1195, 2122, 2552, 3641, 4041
OliI	CACNNNNGTG	1 cut(s)	1829
PaeI	GCATGC	1 cut(s)	2552
PaeR7I	CTCGAG	2 cut(s)	1276, 3566
PciI	ACATGT	1 cut(s)	3637
PciSI	GCTCTTC	2 cut(s)	1524, 2457
PcsI	WCGNNNNNNNCGW	2 cut(s)	243, 461
PctI	GAATGC	4 cut(s)	907, 1666, 2980, 3324
PdiI	GCCGGC	1 cut(s)	597
PdmI	GAANNNNTTC	3 cut(s)	3510, 3967, 4563
PflFI	GACNNNGTC	1 cut(s)	310
PflMI	CCANNNNNTGG	2 cut(s)	3901, 4450
PfoI	TCCNGGA	1 cut(s)	1470
PinAI	ACCGGT	1 cut(s)	135
Ple19I	CGATCG	1 cut(s)	24
PleI	GAGTC	9 cut(s)	1013, 1268, 1832, 1860, 2302, 2424, 3185, 3917, 4030
PluTI	GGCGCC	1 cut(s)	40
PpsI	GAGTC	9 cut(s)	1013, 1268, 1832, 1860, 2302, 2424, 3185, 3917, 4030
PpuMI	RGGWCCY	2 cut(s)	1577, 2726
PscI	ACATGT	1 cut(s)	3637
PsiI	TTATAA	1 cut(s)	3104
Psp124BI	GAGCTC	2 cut(s)	97, 2095
Psp5II	RGGWCCY	2 cut(s)	1577, 2726
Psp6I	CCWGG	6 cut(s)	78, 341, 1855, 2203, 2414, 2905
PspFI	CCCAGC	2 cut(s)	1615, 2314
PspGI	CCWGG	6 cut(s)	78, 341, 1855, 2203, 2414, 2905
PspPPI	RGGWCCY	2 cut(s)	1577, 2726
PspXI	VCTCGAGB	1 cut(s)	1276
PstI	CTGCAG	2 cut(s)	1435, 1936
PsuI	RGATCY	7 cut(s)	925, 1360, 1474, 1492, 3123, 3142, 4603
PsyI	GACNNNGTC	1 cut(s)	310
PvuI	CGATCG	1 cut(s)	24
PvuII	CAGCTG	3 cut(s)	2314, 2470, 4597
RseI	CAYNNNNRTG	5 cut(s)	1829, 2251, 2445, 3780, 4380
SacI	GAGCTC	2 cut(s)	97, 2095
SacII	CCGCGG	2 cut(s)	298, 389
SapI	GCTCTTC	2 cut(s)	1524, 2457
SbfI	CCTGCAGG	1 cut(s)	1435
ScaI	AGTACT	1 cut(s)	3136
SchI	GAGTC	9 cut(s)	1013, 1268, 1832, 1861, 2302, 2424, 3185, 3917, 4031
SdaI	CCTGCAGG	1 cut(s)	1435
SduI	GDGCHC	9 cut(s)	97, 592, 825, 1124, 1519, 2095, 2452, 2524, 3734
SfcI	CTRYAG	4 cut(s)	1431, 1704, 1932, 4264
SfoI	GGCGCC	1 cut(s)	38
Sfr274I	CTCGAG	2 cut(s)	1276, 3566
Sfr303I	CCGCGG	2 cut(s)	298, 389
SgrBI	CCGCGG	2 cut(s)	298, 389
SinI	GGWCC	7 cut(s)	69, 288, 1109, 1577, 2124, 2726, 4570
SlaI	CTCGAG	2 cut(s)	1276, 3566
SmiMI	CAYNNNNRTG	5 cut(s)	1829, 2251, 2445, 3780, 4380
SmlI	CTYRAG	9 cut(s)	1276, 1716, 1966, 2141, 2987, 3070, 3329, 3463, 3566
SmoI	CTYRAG	9 cut(s)	1276, 1716, 1966, 2141, 2987, 3070, 3329, 3463, 3566
SphI	GCATGC	1 cut(s)	2552
Sse8387I	CCTGCAGG	1 cut(s)	1435
SspDI	GGCGCC	1 cut(s)	36
SspMI	CTAG	8 cut(s)	254, 1523, 2355, 2895, 3771, 4044, 4140, 4491
SstI	GAGCTC	2 cut(s)	97, 2095
StyI	CCWWGG	2 cut(s)	253, 2509
TaiI	ACGT	6 cut(s)	311, 467, 2467, 3421, 3769, 4283
TaqI	TCGA	6 cut(s)	237, 455, 1277, 3393, 3567, 3941
TatI	WGTACW	6 cut(s)	1447, 3134, 3309, 4260, 4401, 4419
TscAI	CASTG	6 cut(s)	178, 1074, 1129, 1987, 3096, 4382
TseFI	GTSAC	7 cut(s)	25, 494, 1016, 1065, 2261, 3422, 4534
Tsp45I	GTSAC	7 cut(s)	25, 494, 1016, 1065, 2261, 3422, 4534
TspGWI	ACGGA	5 cut(s)	595, 1874, 2635, 3203, 4421
TspRI	CASTG	6 cut(s)	178, 1074, 1129, 1987, 3096, 4382
Tth111I	GACNNNGTC	1 cut(s)	310
Van91I	CCANNNNNTGG	2 cut(s)	3901, 4450
Vha464I	CTTAAG	1 cut(s)	3463
VpaK11BI	GGWCC	7 cut(s)	69, 288, 1109, 1577, 2124, 2726, 4570
XapI	RAATTY	8 cut(s)	1600, 1643, 1942, 2863, 2932, 3471, 3943, 4521
XceI	RCATGY	6 cut(s)	1084, 1195, 2122, 2552, 3641, 4041
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2118, 4537
XhoI	CTCGAG	2 cut(s)	1276, 3566
XmaJI	CCTAGG	1 cut(s)	253
XmnI	GAANNNNTTC	3 cut(s)	3510, 3967, 4563
XspI	CTAG	8 cut(s)	254, 1523, 2355, 2895, 3771, 4044, 4140, 4491
ZraI	GACGTC	1 cut(s)	309
ZrmI	AGTACT	1 cut(s)	3136
Zsp2I	ATGCAT	1 cut(s)	2980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.