DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 161 |
| AccB1I | GGYRCC | 1 cut(s) | 461 |
| AccI | GTMKAC | 1 cut(s) | 27 |
| AccII | CGCG | 3 cut(s) | 66, 147, 238 |
| AciI | CCGC | 7 cut(s) | 64, 142, 145, 147, 236, 238, 375 |
| AcsI | RAATTY | 1 cut(s) | 661 |
| AcyI | GRCGYC | 1 cut(s) | 158 |
| AfaI | GTAC | 3 cut(s) | 41, 580, 652 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| AgsI | TTSAA | 5 cut(s) | 413, 557, 596, 624, 666 |
| AjiI | CACGTC | 2 cut(s) | 46, 315 |
| AluBI | AGCT | 3 cut(s) | 425, 513, 629 |
| AluI | AGCT | 3 cut(s) | 425, 513, 629 |
| Alw21I | GWGCWC | 1 cut(s) | 631 |
| Alw26I | GTCTC | 1 cut(s) | 464 |
| AoxI | GGCC | 4 cut(s) | 100, 188, 239, 351 |
| ApeKI | GCWGC | 7 cut(s) | 272, 440, 443, 482, 485, 488, 510 |
| ApoI | RAATTY | 1 cut(s) | 661 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 35, 67 |
| AspA2I | CCTAGG | 1 cut(s) | 103 |
| AspLEI | GCGC | 1 cut(s) | 63 |
| AspS9I | GGNCC | 4 cut(s) | 138, 189, 240, 399 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| AvaII | GGWCC | 2 cut(s) | 138, 399 |
| AvrII | CCTAGG | 1 cut(s) | 103 |
| BanI | GGYRCC | 1 cut(s) | 461 |
| BanII | GRGCYC | 1 cut(s) | 631 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 31 |
| BbsI | GAAGAC | 1 cut(s) | 309 |
| Bbv12I | GWGCWC | 1 cut(s) | 631 |
| BbvI | GCAGC | 7 cut(s) | 284, 452, 455, 494, 497, 500, 522 |
| BccI | CCATC | 3 cut(s) | 277, 446, 452 |
| BceAI | ACGGC | 2 cut(s) | 115, 193 |
| BcoDI | GTCTC | 1 cut(s) | 464 |
| BfaI | CTAG | 1 cut(s) | 104 |
| BfoI | RGCGCY | 1 cut(s) | 64 |
| BlnI | CCTAGG | 1 cut(s) | 103 |
| Bme18I | GGWCC | 2 cut(s) | 138, 399 |
| BmgBI | CACGTC | 2 cut(s) | 46, 315 |
| BmgT120I | GGNCC | 4 cut(s) | 138, 189, 240, 399 |
| BmiI | GGNNCC | 4 cut(s) | 140, 234, 400, 463 |
| BmrI | ACTGGG | 1 cut(s) | 411 |
| BmuI | ACTGGG | 1 cut(s) | 411 |
| BpiI | GAAGAC | 1 cut(s) | 309 |
| BpuEI | CTTGAG | 1 cut(s) | 615 |
| BsaHI | GRCGYC | 1 cut(s) | 158 |
| BsaJI | CCNNGG | 3 cut(s) | 103, 145, 236 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| Bse1I | ACTGG | 2 cut(s) | 140, 417 |
| Bse3DI | GCAATG | 1 cut(s) | 498 |
| BseDI | CCNNGG | 3 cut(s) | 103, 145, 236 |
| BseGI | GGATG | 4 cut(s) | 40, 97, 172, 650 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| BseMI | GCAATG | 1 cut(s) | 498 |
| BseMII | CTCAG | 1 cut(s) | 459 |
| BseNI | ACTGG | 2 cut(s) | 140, 417 |
| BseRI | GAGGAG | 2 cut(s) | 83, 211 |
| BseXI | GCAGC | 7 cut(s) | 284, 452, 455, 494, 497, 500, 522 |
| Bsh1236I | CGCG | 3 cut(s) | 66, 147, 238 |
| Bsh1285I | CGRYCG | 1 cut(s) | 27 |
| BshFI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| BshNI | GGYRCC | 1 cut(s) | 461 |
| BsiEI | CGRYCG | 1 cut(s) | 27 |
| BsiHKAI | GWGCWC | 1 cut(s) | 631 |
| BslFI | GGGAC | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| BsmAI | GTCTC | 1 cut(s) | 464 |
| BsmFI | GGGAC | 1 cut(s) | 92 |
| BsnI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| Bsp1286I | GDGCHC | 1 cut(s) | 631 |
| Bsp143I | GATC | 2 cut(s) | 12, 48 |
| BspACI | CCGC | 7 cut(s) | 64, 142, 145, 147, 236, 238, 375 |
| BspANI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| BspCNI | CTCAG | 1 cut(s) | 460 |
| BspFNI | CGCG | 3 cut(s) | 66, 147, 238 |
| BspLI | GGNNCC | 4 cut(s) | 140, 234, 400, 463 |
| BspQI | GCTCTTC | 1 cut(s) | 572 |
| BspT107I | GGYRCC | 1 cut(s) | 461 |
| BsrDI | GCAATG | 1 cut(s) | 498 |
| BsrI | ACTGG | 2 cut(s) | 140, 417 |
| BssECI | CCNNGG | 3 cut(s) | 103, 145, 236 |
| BssMI | GATC | 2 cut(s) | 12, 48 |
| BssNI | GRCGYC | 1 cut(s) | 158 |
| BssT1I | CCWWGG | 1 cut(s) | 103 |
| Bst4CI | ACNGT | 2 cut(s) | 25, 122 |
| Bst6I | CTCTTC | 2 cut(s) | 123, 572 |
| BstACI | GRCGYC | 1 cut(s) | 158 |
| BstC8I | GCNNGC | 2 cut(s) | 149, 543 |
| BstDEI | CTNAG | 1 cut(s) | 468 |
| BstDSI | CCRYGG | 2 cut(s) | 145, 236 |
| BstF5I | GGATG | 4 cut(s) | 40, 97, 172, 650 |
| BstFNI | CGCG | 3 cut(s) | 66, 147, 238 |
| BstH2I | RGCGCY | 1 cut(s) | 64 |
| BstHHI | GCGC | 1 cut(s) | 63 |
| BstKTI | GATC | 2 cut(s) | 15, 51 |
| BstMAI | GTCTC | 1 cut(s) | 464 |
| BstMBI | GATC | 2 cut(s) | 12, 48 |
| BstMCI | CGRYCG | 1 cut(s) | 27 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 247, 488 |
| BstNSI | RCATGY | 1 cut(s) | 565 |
| BstUI | CGCG | 3 cut(s) | 66, 147, 238 |
| BstV1I | GCAGC | 7 cut(s) | 284, 452, 455, 494, 497, 500, 522 |
| BstV2I | GAAGAC | 1 cut(s) | 309 |
| BsuRI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| BtgI | CCRYGG | 2 cut(s) | 145, 236 |
| BtrI | CACGTC | 2 cut(s) | 46, 315 |
| BtsCI | GGATG | 4 cut(s) | 40, 97, 172, 650 |
| Cac8I | GCNNGC | 2 cut(s) | 149, 543 |
| CfoI | GCGC | 1 cut(s) | 63 |
| Cfr13I | GGNCC | 4 cut(s) | 138, 189, 240, 399 |
| Cfr42I | CCGCGG | 2 cut(s) | 148, 239 |
| CseI | GACGC | 1 cut(s) | 487 |
| Csp6I | GTAC | 3 cut(s) | 40, 579, 651 |
| CviAII | CATG | 1 cut(s) | 562 |
| CviQI | GTAC | 3 cut(s) | 40, 579, 651 |
| DdeI | CTNAG | 1 cut(s) | 468 |
| DpnI | GATC | 2 cut(s) | 14, 50 |
| DpnII | GATC | 2 cut(s) | 12, 48 |
| Eam1104I | CTCTTC | 2 cut(s) | 123, 572 |
| EarI | CTCTTC | 2 cut(s) | 123, 572 |
| Ecl136II | GAGCTC | 1 cut(s) | 629 |
| Eco130I | CCWWGG | 1 cut(s) | 103 |
| Eco24I | GRGCYC | 1 cut(s) | 631 |
| Eco47I | GGWCC | 2 cut(s) | 138, 399 |
| Eco53kI | GAGCTC | 1 cut(s) | 629 |
| EcoICRI | GAGCTC | 1 cut(s) | 629 |
| EcoT14I | CCWWGG | 1 cut(s) | 103 |
| EcoT38I | GRGCYC | 1 cut(s) | 631 |
| ErhI | CCWWGG | 1 cut(s) | 103 |
| FaeI | CATG | 1 cut(s) | 565 |
| FaiI | YATR | 3 cut(s) | 185, 527, 563 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 348, 380 |
| FaqI | GGGAC | 1 cut(s) | 92 |
| FatI | CATG | 1 cut(s) | 561 |
| FauI | CCCGC | 2 cut(s) | 140, 149 |
| FblI | GTMKAC | 1 cut(s) | 27 |
| FokI | GGATG | 4 cut(s) | 47, 104, 179, 657 |
| FriOI | GRGCYC | 1 cut(s) | 631 |
| FspBI | CTAG | 1 cut(s) | 104 |
| GlaI | GCGC | 1 cut(s) | 62 |
| HaeII | RGCGCY | 1 cut(s) | 64 |
| HaeIII | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| HgaI | GACGC | 1 cut(s) | 487 |
| HhaI | GCGC | 1 cut(s) | 63 |
| Hin1I | GRCGYC | 1 cut(s) | 158 |
| Hin1II | CATG | 1 cut(s) | 565 |
| Hin6I | GCGC | 1 cut(s) | 61 |
| HinP1I | GCGC | 1 cut(s) | 61 |
| HincII | GTYRAC | 1 cut(s) | 28 |
| HindII | GTYRAC | 1 cut(s) | 28 |
| HinfI | GANTC | 1 cut(s) | 170 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 2 cut(s) | 28, 312 |
| Hpy188I | TCNGA | 6 cut(s) | 156, 166, 175, 469, 503, 520 |
| Hpy188III | TCNNGA | 3 cut(s) | 554, 624, 632 |
| Hpy8I | GTNNAC | 2 cut(s) | 28, 312 |
| Hpy99I | CGWCG | 7 cut(s) | 32, 47, 122, 160, 262, 280, 503 |
| HpyAV | CCTTC | 4 cut(s) | 199, 211, 217, 223 |
| HpyCH4III | ACNGT | 2 cut(s) | 25, 122 |
| HpyCH4IV | ACGT | 3 cut(s) | 45, 158, 314 |
| HpyCH4V | TGCA | 3 cut(s) | 114, 545, 610 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 247, 488 |
| HpyF3I | CTNAG | 1 cut(s) | 468 |
| HpySE526I | ACGT | 3 cut(s) | 45, 158, 314 |
| Hsp92I | GRCGYC | 1 cut(s) | 158 |
| Hsp92II | CATG | 1 cut(s) | 565 |
| HspAI | GCGC | 1 cut(s) | 61 |
| KspI | CCGCGG | 2 cut(s) | 148, 239 |
| Kzo9I | GATC | 2 cut(s) | 12, 48 |
| LguI | GCTCTTC | 1 cut(s) | 572 |
| LmnI | GCTCC | 1 cut(s) | 232 |
| LpnPI | CCDG | 5 cut(s) | 61, 121, 430, 521, 575 |
| Lsp1109I | GCAGC | 7 cut(s) | 284, 452, 455, 494, 497, 500, 522 |
| MaeI | CTAG | 1 cut(s) | 104 |
| MaeII | ACGT | 3 cut(s) | 45, 158, 314 |
| MalI | GATC | 2 cut(s) | 14, 50 |
| MboI | GATC | 2 cut(s) | 12, 48 |
| MboII | GAAGA | 7 cut(s) | 43, 140, 214, 226, 309, 443, 559 |
| MhlI | GDGCHC | 1 cut(s) | 631 |
| MluCI | AATT | 2 cut(s) | 253, 661 |
| MmeI | TCCRAC | 2 cut(s) | 179, 481 |
| MseI | TTAA | 1 cut(s) | 327 |
| MspA1I | CMGCKG | 2 cut(s) | 147, 238 |
| MvnI | CGCG | 3 cut(s) | 66, 147, 238 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 247, 488 |
| NdeII | GATC | 2 cut(s) | 12, 48 |
| NlaIII | CATG | 1 cut(s) | 565 |
| NlaIV | GGNNCC | 4 cut(s) | 140, 234, 400, 463 |
| NspI | RCATGY | 1 cut(s) | 565 |
| PciSI | GCTCTTC | 1 cut(s) | 572 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 24, 272 |
| PfeI | GAWTC | 1 cut(s) | 170 |
| PflFI | GACNNNGTC | 1 cut(s) | 23 |
| Psp124BI | GAGCTC | 1 cut(s) | 631 |
| PspN4I | GGNNCC | 4 cut(s) | 140, 234, 400, 463 |
| PspPI | GGNCC | 4 cut(s) | 138, 189, 240, 399 |
| PsyI | GACNNNGTC | 1 cut(s) | 23 |
| RsaI | GTAC | 3 cut(s) | 41, 580, 652 |
| RsaNI | GTAC | 3 cut(s) | 40, 579, 651 |
| SacI | GAGCTC | 1 cut(s) | 631 |
| SacII | CCGCGG | 2 cut(s) | 148, 239 |
| SalI | GTCGAC | 1 cut(s) | 26 |
| SapI | GCTCTTC | 1 cut(s) | 572 |
| SaqAI | TTAA | 1 cut(s) | 327 |
| Sau3AI | GATC | 2 cut(s) | 12, 48 |
| Sau96I | GGNCC | 4 cut(s) | 138, 189, 240, 399 |
| SduI | GDGCHC | 1 cut(s) | 631 |
| SetI | ASST | 9 cut(s) | 48, 161, 210, 317, 427, 515, 525, 622, 631 |
| Sfr303I | CCGCGG | 2 cut(s) | 148, 239 |
| SgrBI | CCGCGG | 2 cut(s) | 148, 239 |
| SinI | GGWCC | 2 cut(s) | 138, 399 |
| SmlI | CTYRAG | 1 cut(s) | 630 |
| SmoI | CTYRAG | 1 cut(s) | 630 |
| Sse9I | AATT | 2 cut(s) | 253, 661 |
| SsiI | CCGC | 7 cut(s) | 64, 142, 145, 147, 236, 238, 375 |
| SspMI | CTAG | 1 cut(s) | 104 |
| SstI | GAGCTC | 1 cut(s) | 631 |
| StyI | CCWWGG | 1 cut(s) | 103 |
| TaaI | ACNGT | 2 cut(s) | 25, 122 |
| TaiI | ACGT | 3 cut(s) | 48, 161, 317 |
| TaqI | TCGA | 2 cut(s) | 27, 87 |
| TasI | AATT | 2 cut(s) | 253, 661 |
| TatI | WGTACW | 1 cut(s) | 578 |
| TauI | GCSGC | 5 cut(s) | 66, 147, 238, 241, 377 |
| TfiI | GAWTC | 1 cut(s) | 170 |
| Tru1I | TTAA | 1 cut(s) | 327 |
| Tru9I | TTAA | 1 cut(s) | 327 |
| TseI | GCWGC | 7 cut(s) | 272, 440, 443, 482, 485, 488, 510 |
| TspDTI | ATGAA | 5 cut(s) | 73, 183, 377, 444, 450 |
| TspGWI | ACGGA | 1 cut(s) | 281 |
| Tth111I | GACNNNGTC | 1 cut(s) | 23 |
| VpaK11BI | GGWCC | 2 cut(s) | 138, 399 |
| XapI | RAATTY | 1 cut(s) | 661 |
| XceI | RCATGY | 1 cut(s) | 565 |
| XmaJI | CCTAGG | 1 cut(s) | 103 |
| XmiI | GTMKAC | 1 cut(s) | 27 |
| XspI | CTAG | 1 cut(s) | 104 |
| ZraI | GACGTC | 1 cut(s) | 159 |