Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Reverse (-)

28281052 .. 28290807

Gene Structure 9756 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018320

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4473 bp

ATGGAGAAGCCGATCTACGATACGGTCGACGAGGACGAGTACGACGCGATCATCGCCAAGCGGCGCGAGGAGTTCGGGGACTTCATCGAGGACGACGACGGCCTAGGGTACCGCGACGACGGCGAGGAAGAGGACTGGACCCGCCGCGGGCTTCCGACGTCCTCAGATGAATCGGACGGCGATATGAGGCCCAGGAGGAGGAAGAAGGTGGAGAAGAAGGAGAAGGAGAAGGAGCCGCGGCCCAAAAAGCCCAATTCGTCACTTACGGCGGCGGCGGCGATGATGGGGAAGCAGAGGCTTTCGTCGATGTTCACGTCGTCAGTGTTTAATAAAAGTAGGGATAGTGACAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCTGAGTTTGCACCGGACGAGGCTGATAGAGAGAAACGGAGGAGGGCACAGCCGGCAAGGAGTTTTGTTCCGATTACCGGAGTTAAGAGTGAGAGAGTGAGTAATGGGGGTGTTGAGTTATCAAGGAGGCTTGAATTGGATAGAATCGTTGCAAATGGTAGTGGTTCTGTGCATAGTAAGGAAATGGATAAAGATTCTGAGGGCAATAAGGAGTTGGATTTCAGAAATTGTCAGCCCCAAATTGAGCAGTTCCATGATTTTTCGGAGAATAGTGAGGTGCCCGAGGAGAAGAAGACCGAGGATTCTGCTGAGGTGCAGGCGGAGCCTGTGGTGAAGAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATGAGGATCCGGCGTTGAGTGCTATGGCAGGATGGAAGGCCGCAATGACTGGTGGGGCCGGGGTTACTGCTGGCGGCGGCAATGTGGAGGTCAAAAGTGACTCAATTAGTGAAGGGAGTTCGGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAATATGGGCACTGTTTATCTATTTGGGAAGGTTAAAGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTAAAGAACATGCAAAGATGTGTCTATGCAATTCCGGACAGTCCTTTATTTCAAACTGACGAAATGATGAAGCTTGAAAAGGATGCCGAGGACTCAAGGATTTCACCCACAGATTTTCGTAAGAAGCTGCATGATGCGGCTTCAGAATTAAAGATTGAATTAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGGCACCTGTCAAGAGGAACTATGCTTTTGAGCGATCTGACATACCTGCAGGGGAGAATTATGTACTGAAGATCAATTATCCATTCCGGGATCCACCACTATCAGCAGATCTAAAAGGAGAAACTTTTTGTGCTCTTCTAGGAACTCATAGCAGCGCGTTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAAATTTTTCCATCTGCCCAGCTCCACAACGGGTAAGCTGGTGCAAATTTGAGGTGATCGTGAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACAAAGAAAACTGTAGATATTCCTCAAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTATCAATGAGAAGCAGAATGTTAACGAAATTGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCCGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGTGGCATATTTCCTATGGGGTTTACTAAAGTGGCTGCAGATAAGAATTCTAAAGCCGGATCAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTAAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTTGTTGGACATAATATATCTGGATTTGATCTGGATGTTCTTCTCCACAGAGCTCAGGTTTGCAGGGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATCATGTCCTGCATTGCTGGTCGGCTGTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAAGGAGGTCAGCTATTCCTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCGCATGATATTCCTAGAATGTTACAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCCTGGTTGTCTATGGGACTCATGTTTCATTTGAGTGCTCTTCCCCTCACACGTCAGCTGACTAATATAAGCGGCAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTAGAATATCTCTTACTGCATGCTTTTCATGCAAAAAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGCGTTGAGGAAAAAGCTGCTGATGAGTTGGATGTAAATGATGTAAATGTTGATAATGATGCACAGAGTGGTCATGGAAAAGGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTATTGGAGCCAAAAAAAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATATGCTTCACAACTGTTGAAAGAAATTCAGAAGGATTGGTTTCTCATTTACCTTCTAGTCAAAGACCTGGACTTTTACCTGAGTTGTTAAAAAATTTAGTTGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAACTTGACATTCAACAGCAAGCTCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAACTCAAGATTTTATGCAAAGCCACTGGCAGAGCTTATAACACTACGGGGCAGGGAGATCCTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTAATCTATGGCGATACGGACTCAATAATGATTTATAGTGGGCTGGATGATAGTGTGAAAGCAAAAGCAATTGCTGTGAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGATGGCTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCGGCTGTGAAGGAGCTAACGAAAAACGAGAAAAAATACGAGGTTATCGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGTTTACTTTCAAAGGAACTGGGAGATTTCTGCTTAAGTCAAATTCTGTCAGGAGGGTCATCTGAAGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTGCCCTCGAGAAATATATCATCACCAAGACACTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAGTGGTTACTCTACTGGTTGCTCTGTTGGAGATACAGTCCCTTATATTATTTGCTGTGAGCAGGGCACAAGTTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAGATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCTGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACGAGCCCCCAACGCTTGGCTGATTGTTTAGGGATTGACTCATCCAAGTTCAAATCGAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTGTTGATGATGAAGAGAGATATCGGGATTGCGAGTCTTTGATCTTAGCATGTCCTAGCTGCTCCGGTACTTTTGATTGTCCAGCCATCTTCAACTACATTGCCAAGTCAATCAATGAAAAGCCAACAAGGCCACAAGGAGAAGAATCTTCCAGCGATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCCAATCAGGTCAAAAGGCAAGCAGAGGGTTTTGTTTCAATGTACTACAGGGGCATAATGACGTGTGATGATGAAACTTGCAAATACACCACACGAAGTCTCAACCTCCGGCTTGTTGGTGATGCTGAGAGAGGAACAGTCTGTCCAAACTATCCTCGCTGCAATGGGCATCTTGTAAGAAAGTACACGGAAGCTGATTTGTACAAACAGCTCTCATTTTTCTGCCATGTCTTGGATACAGTACGCTGCGTAGAAAAGATGGAACCTAAGACTAGACTGCCGGTAGAGTTAGAGTTGGCCAAAATTCGCCCAATTGTGACTTTGGCTGCCTCAACAGTCGAAAGGATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTGCAAGATCTTTTTGTTACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1491

Amino Acids

166.77

Weight (kDa)

6.87

Isoelectric Point (pI)

45.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	2 - 46	6.7e-13	DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1	PF03104	461 - 715	2.1e-26	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	781 - 1243	2.8e-125	DNA polymerase family B
zf-DNA_Pol	PF08996	1281 - 1487	1.1e-42	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2954
AatII	GACGTC	1 cut(s)	161
Acc36I	ACCTGC	1 cut(s)	1288
Acc65I	GGTACC	1 cut(s)	108
AccB1I	GGYRCC	4 cut(s)	108, 670, 1237, 1957
AccB7I	CCANNNNNTGG	2 cut(s)	3751, 4300
AccI	GTMKAC	1 cut(s)	27
AccII	CGCG	6 cut(s)	47, 66, 114, 147, 238, 1391
AccIII	TCCGGA	1 cut(s)	1066
AclWI	GGATC	9 cut(s)	770, 783, 1218, 1319, 1332, 1813, 2066, 2968, 3000
AcoI	YGGCCR	1 cut(s)	4365
AcsI	RAATTY	8 cut(s)	1450, 1493, 1792, 2713, 2782, 3321, 3793, 4371
AcuI	CTGAAG	5 cut(s)	1158, 1322, 2468, 2894, 3363
AcyI	GRCGYC	1 cut(s)	158
AdeI	CACNNNGTG	1 cut(s)	2552
AflII	CTTAAG	1 cut(s)	3313
AflIII	ACRYGT	3 cut(s)	2312, 3487, 4130
AjiI	CACGTC	3 cut(s)	315, 2315, 4131
AjnI	CCWGG	5 cut(s)	191, 1705, 2053, 2264, 2755
AleI	CACNNNNGTG	1 cut(s)	1679
AloI	GAACNNNNNNTCC	2 cut(s)	4195, 4227
Alw21I	GWGCWC	3 cut(s)	1369, 1945, 2302
Alw26I	GTCTC	6 cut(s)	1537, 1639, 2249, 2840, 4059, 4172
AlwI	GGATC	9 cut(s)	770, 783, 1218, 1319, 1332, 1813, 2066, 2968, 3000
Ama87I	CYCGRG	2 cut(s)	674, 3416
Aor13HI	TCCGGA	1 cut(s)	1066
ApoI	RAATTY	8 cut(s)	1450, 1493, 1792, 2713, 2782, 3321, 3793, 4371
Asp700I	GAANNNNTTC	3 cut(s)	3360, 3817, 4413
Asp718I	GGTACC	1 cut(s)	108
AspA2I	CCTAGG	1 cut(s)	103
AspLEI	GCGC	4 cut(s)	66, 1391, 3502, 4014
AsuC2I	CCSGG	3 cut(s)	829, 1322, 3604
AsuHPI	GGTGA	6 cut(s)	736, 1128, 1513, 3424, 4049, 4199
AvaI	CYCGRG	2 cut(s)	674, 3416
AvaII	GGWCC	6 cut(s)	138, 959, 1427, 1974, 2576, 4420
AvrII	CCTAGG	1 cut(s)	103
BaeGI	GKGCMC	5 cut(s)	442, 675, 974, 2374, 3584
BalI	TGGCCA	1 cut(s)	4367
BamHI	GGATCC	2 cut(s)	775, 1324
BanI	GGYRCC	4 cut(s)	108, 670, 1237, 1957
BanII	GRGCYC	2 cut(s)	1945, 3743
BarI	GAAGNNNNNNTAC	1 cut(s)	31
BbsI	GAAGAC	1 cut(s)	692
Bbv12I	GWGCWC	3 cut(s)	1369, 1945, 2302
BbvCI	CCTCAGC	1 cut(s)	702
BceAI	ACGGC	4 cut(s)	115, 136, 193, 282
BcgI	CGANNNNNNTGC	4 cut(s)	680, 714, 2243, 2277
BciT130I	CCWGG	5 cut(s)	193, 1707, 2055, 2266, 2757
BciVI	GTATCC	3 cut(s)	2446, 4297, 4434
BcnI	CCSGG	3 cut(s)	829, 1322, 3604
BcoDI	GTCTC	6 cut(s)	1537, 1639, 2249, 2840, 4059, 4172
BfaI	CTAG	7 cut(s)	104, 1373, 2205, 2745, 3621, 3894, 4341
BfmI	CTRYAG	4 cut(s)	1281, 1554, 1782, 4114
BfrI	CTTAAG	1 cut(s)	3313
BfuAI	ACCTGC	1 cut(s)	1288
BfuI	GTATCC	3 cut(s)	2446, 4297, 4434
BglI	GCCNNNNNGGC	1 cut(s)	3967
BglII	AGATCT	2 cut(s)	1342, 4453
BlnI	CCTAGG	1 cut(s)	103
BlpI	GCTNAGC	1 cut(s)	1715
BmcAI	AGTACT	1 cut(s)	2986
Bme1390I	CCNGG	8 cut(s)	193, 829, 1322, 1707, 2055, 2266, 2757, 3604
Bme18I	GGWCC	6 cut(s)	138, 959, 1427, 1974, 2576, 4420
BmeT110I	CYCGRG	2 cut(s)	674, 3416
BmgBI	CACGTC	3 cut(s)	315, 2315, 4131
BmrFI	CCNGG	8 cut(s)	193, 829, 1322, 1707, 2055, 2266, 2757, 3604
BmrI	ACTGGG	1 cut(s)	3308
BmuI	ACTGGG	1 cut(s)	3308
BpiI	GAAGAC	1 cut(s)	692
BpmI	CTGGAG	2 cut(s)	2064, 3298
Bpu10I	CCTNAGC	3 cut(s)	702, 1944, 2010
Bpu1102I	GCTNAGC	1 cut(s)	1715
BpuEI	CTTGAG	7 cut(s)	1111, 1551, 1837, 1976, 2822, 2905, 3164
BpuMI	CCSGG	3 cut(s)	829, 1322, 3604
BsaBI	GATNNNNATC	2 cut(s)	774, 897
BsaHI	GRCGYC	1 cut(s)	158
BsaI	GGTCTC	2 cut(s)	1537, 2840
BsaWI	WCCGGW	5 cut(s)	406, 470, 1066, 2175, 3902
BsaXI	ACNNNNNCTCC	2 cut(s)	3537, 3567
Bse118I	RCCGGY	2 cut(s)	445, 4348
Bse1I	ACTGG	6 cut(s)	140, 823, 2946, 3281, 3303, 3535
Bse3DI	GCAATG	5 cut(s)	819, 856, 2070, 3936, 4237
Bse8I	GATNNNNATC	2 cut(s)	774, 897
BseAI	TCCGGA	1 cut(s)	1066
BseBI	CCWGG	5 cut(s)	193, 1707, 2055, 2266, 2757
BseJI	GATNNNNATC	2 cut(s)	774, 897
BseMI	GCAATG	5 cut(s)	819, 856, 2070, 3936, 4237
BseNI	ACTGG	6 cut(s)	140, 823, 2946, 3281, 3303, 3535
BseRI	GAGGAG	5 cut(s)	83, 211, 448, 692, 2812
BseSI	GKGCMC	5 cut(s)	442, 675, 974, 2374, 3584
BseYI	CCCAGC	2 cut(s)	1465, 2164
BsgI	GTGCAG	2 cut(s)	728, 4024
Bsh1236I	CGCG	6 cut(s)	47, 66, 114, 147, 238, 1391
Bsh1285I	CGRYCG	1 cut(s)	27
BshNI	GGYRCC	4 cut(s)	108, 670, 1237, 1957
BsiEI	CGRYCG	1 cut(s)	27
BsiHKAI	GWGCWC	3 cut(s)	1369, 1945, 2302
BsiHKCI	CYCGRG	2 cut(s)	674, 3416
BslFI	GGGAC	5 cut(s)	92, 2292, 3539, 3670, 4433
BsmAI	GTCTC	6 cut(s)	1537, 1639, 2249, 2840, 4059, 4172
BsmFI	GGGAC	5 cut(s)	92, 2292, 3539, 3670, 4433
BsmI	GAATGC	4 cut(s)	757, 1516, 2830, 3174
Bso31I	GGTCTC	2 cut(s)	1537, 2840
BsoBI	CYCGRG	2 cut(s)	674, 3416
Bsp1286I	GDGCHC	9 cut(s)	442, 675, 974, 1369, 1945, 2302, 2374, 3584, 3743
Bsp13I	TCCGGA	1 cut(s)	1066
Bsp1407I	TGTACA	2 cut(s)	3159, 4269
Bsp1720I	GCTNAGC	1 cut(s)	1715
BspEI	TCCGGA	1 cut(s)	1066
BspFNI	CGCG	6 cut(s)	47, 66, 114, 147, 238, 1391
BspMAI	CTGCAG	2 cut(s)	1285, 1786
BspMI	ACCTGC	1 cut(s)	1288
BspPI	GGATC	9 cut(s)	770, 783, 1218, 1319, 1332, 1813, 2066, 2968, 3000
BspQI	GCTCTTC	2 cut(s)	1374, 2307
BspT107I	GGYRCC	4 cut(s)	108, 670, 1237, 1957
BspTI	CTTAAG	1 cut(s)	3313
BspTNI	GGTCTC	2 cut(s)	1537, 2840
BsrDI	GCAATG	5 cut(s)	819, 856, 2070, 3936, 4237
BsrFI	RCCGGY	2 cut(s)	445, 4348
BsrGI	TGTACA	2 cut(s)	3159, 4269
BsrI	ACTGG	6 cut(s)	140, 823, 2946, 3281, 3303, 3535
BssAI	RCCGGY	2 cut(s)	445, 4348
BssNI	GRCGYC	1 cut(s)	158
BssT1I	CCWWGG	2 cut(s)	103, 2359
Bst2UI	CCWGG	5 cut(s)	193, 1707, 2055, 2266, 2757
Bst6I	CTCTTC	7 cut(s)	123, 729, 942, 1374, 1694, 2307, 3846
BstACI	GRCGYC	1 cut(s)	158
BstAFI	CTTAAG	1 cut(s)	3313
BstAUI	TGTACA	2 cut(s)	3159, 4269
BstC8I	GCNNGC	8 cut(s)	149, 447, 711, 841, 2016, 2400, 2868, 4089
BstDSI	CCRYGG	2 cut(s)	145, 236
BstFNI	CGCG	6 cut(s)	47, 66, 114, 147, 238, 1391
BstHHI	GCGC	4 cut(s)	66, 1391, 3502, 4014
BstMAI	GTCTC	6 cut(s)	1537, 1639, 2249, 2840, 4059, 4172
BstMCI	CGRYCG	1 cut(s)	27
BstNI	CCWGG	5 cut(s)	193, 1707, 2055, 2266, 2757
BstNSI	RCATGY	6 cut(s)	934, 1045, 1972, 2402, 3491, 3891
BstSCI	CCNGG	8 cut(s)	191, 827, 1320, 1705, 2053, 2264, 2755, 3602
BstSFI	CTRYAG	4 cut(s)	1281, 1554, 1782, 4114
BstSLI	GKGCMC	5 cut(s)	442, 675, 974, 2374, 3584
BstUI	CGCG	6 cut(s)	47, 66, 114, 147, 238, 1391
BstV2I	GAAGAC	1 cut(s)	692
BstX2I	RGATCY	7 cut(s)	775, 1210, 1324, 1342, 2973, 2992, 4453
BstYI	RGATCY	7 cut(s)	775, 1210, 1324, 1342, 2973, 2992, 4453
BsuI	GTATCC	3 cut(s)	2446, 4297, 4434
BtgI	CCRYGG	2 cut(s)	145, 236
BtgZI	GCGATG	2 cut(s)	37, 293
BtrI	CACGTC	3 cut(s)	315, 2315, 4131
BtsI	GCAGTG	2 cut(s)	917, 1837
BtsIMutI	CAGTG	5 cut(s)	327, 917, 972, 1837, 2939
BveI	ACCTGC	1 cut(s)	1288
Cac8I	GCNNGC	8 cut(s)	149, 447, 711, 841, 2016, 2400, 2868, 4089
CfoI	GCGC	4 cut(s)	66, 1391, 3502, 4014
Cfr10I	RCCGGY	2 cut(s)	445, 4348
Cfr42I	CCGCGG	2 cut(s)	148, 239
CseI	GACGC	1 cut(s)	53
DraIII	CACNNNGTG	1 cut(s)	2552
EaeI	YGGCCR	1 cut(s)	4365
Eam1104I	CTCTTC	7 cut(s)	123, 729, 942, 1374, 1694, 2307, 3846
EarI	CTCTTC	7 cut(s)	123, 729, 942, 1374, 1694, 2307, 3846
EciI	GGCGGA	1 cut(s)	728
Ecl136II	GAGCTC	1 cut(s)	1943
Eco130I	CCWWGG	2 cut(s)	103, 2359
Eco24I	GRGCYC	2 cut(s)	1945, 3743
Eco31I	GGTCTC	2 cut(s)	1537, 2840
Eco32I	GATATC	1 cut(s)	3860
Eco47I	GGWCC	6 cut(s)	138, 959, 1427, 1974, 2576, 4420
Eco53kI	GAGCTC	1 cut(s)	1943
Eco57I	CTGAAG	5 cut(s)	1158, 1322, 2468, 2894, 3363
Eco88I	CYCGRG	2 cut(s)	674, 3416
EcoICRI	GAGCTC	1 cut(s)	1943
EcoO109I	RGGNCCY	3 cut(s)	1427, 2576, 3253
EcoRI	GAATTC	2 cut(s)	1792, 3793
EcoRII	CCWGG	5 cut(s)	191, 1705, 2053, 2264, 2755
EcoRV	GATATC	1 cut(s)	3860
EcoT14I	CCWWGG	2 cut(s)	103, 2359
EcoT22I	ATGCAT	1 cut(s)	2830
EcoT38I	GRGCYC	2 cut(s)	1945, 3743
ErhI	CCWWGG	2 cut(s)	103, 2359
FaqI	GGGAC	5 cut(s)	92, 2292, 3539, 3670, 4433
FauI	CCCGC	2 cut(s)	140, 149
FblI	GTMKAC	1 cut(s)	27
FriOI	GRGCYC	2 cut(s)	1945, 3743
FspBI	CTAG	7 cut(s)	104, 1373, 2205, 2745, 3621, 3894, 4341
GlaI	GCGC	4 cut(s)	65, 1390, 3501, 4013
GsaI	CCCAGC	2 cut(s)	1469, 2168
GsuI	CTGGAG	2 cut(s)	2064, 3298
HgaI	GACGC	1 cut(s)	53
HhaI	GCGC	4 cut(s)	66, 1391, 3502, 4014
Hin1I	GRCGYC	1 cut(s)	158
Hin6I	GCGC	4 cut(s)	64, 1389, 3500, 4012
HinP1I	GCGC	4 cut(s)	64, 1389, 3500, 4012
HincII	GTYRAC	3 cut(s)	28, 1624, 3118
HindII	GTYRAC	3 cut(s)	28, 1624, 3118
HindIII	AAGCTT	3 cut(s)	1103, 1739, 2012
HpaI	GTTAAC	2 cut(s)	1624, 3118
HphI	GGTGA	6 cut(s)	736, 1128, 1513, 3424, 4049, 4199
Hpy166II	GTNNAC	7 cut(s)	28, 312, 1624, 1770, 3118, 3284, 4254
Hpy8I	GTNNAC	7 cut(s)	28, 312, 1624, 1770, 3118, 3284, 4254
HpyCH4IV	ACGT	6 cut(s)	158, 314, 2314, 3268, 3616, 4130
HpySE526I	ACGT	6 cut(s)	158, 314, 2314, 3268, 3616, 4130
Hsp92I	GRCGYC	1 cut(s)	158
HspAI	GCGC	4 cut(s)	64, 1389, 3500, 4012
Kpn2I	TCCGGA	1 cut(s)	1066
KpnI	GGTACC	1 cut(s)	112
KroI	GCCGGC	1 cut(s)	445
KroNI	GCCGGC	1 cut(s)	447
KspAI	GTTAAC	2 cut(s)	1624, 3118
KspI	CCGCGG	2 cut(s)	148, 239
LguI	GCTCTTC	2 cut(s)	1374, 2307
LmnI	GCTCC	8 cut(s)	232, 715, 1474, 1519, 2045, 2605, 3208, 3905
MaeI	CTAG	7 cut(s)	104, 1373, 2205, 2745, 3621, 3894, 4341
MaeII	ACGT	6 cut(s)	158, 314, 2314, 3268, 3616, 4130
MfeI	CAATTG	2 cut(s)	3090, 4380
MflI	RGATCY	7 cut(s)	775, 1210, 1324, 1342, 2973, 2992, 4453
MhlI	GDGCHC	9 cut(s)	442, 675, 974, 1369, 1945, 2302, 2374, 3584, 3743
MlsI	TGGCCA	1 cut(s)	4367
MluNI	TGGCCA	1 cut(s)	4367
MlyI	GAGTC	9 cut(s)	863, 1118, 1682, 1711, 2152, 2274, 3035, 3767, 3881
MmeI	TCCRAC	8 cut(s)	179, 588, 1188, 1374, 1857, 1876, 2493, 3523
Mox20I	TGGCCA	1 cut(s)	4367
Mph1103I	ATGCAT	1 cut(s)	2830
MroI	TCCGGA	1 cut(s)	1066
MroNI	GCCGGC	1 cut(s)	445
MroXI	GAANNNNTTC	3 cut(s)	3360, 3817, 4413
MscI	TGGCCA	1 cut(s)	4367
MslI	CAYNNNNRTG	4 cut(s)	1679, 2101, 2295, 3630
Msp20I	TGGCCA	1 cut(s)	4367
MspA1I	CMGCKG	5 cut(s)	147, 238, 2164, 2320, 4447
MspCI	CTTAAG	1 cut(s)	3313
MspR9I	CCNGG	8 cut(s)	193, 829, 1322, 1707, 2055, 2266, 2757, 3604
MunI	CAATTG	2 cut(s)	3090, 4380
Mva1269I	GAATGC	4 cut(s)	757, 1516, 2830, 3174
MvaI	CCWGG	5 cut(s)	193, 1707, 2055, 2266, 2757
MvnI	CGCG	6 cut(s)	47, 66, 114, 147, 238, 1391
NaeI	GCCGGC	1 cut(s)	447
NciI	CCSGG	3 cut(s)	829, 1322, 3604
NgoMIV	GCCGGC	1 cut(s)	445
NmeAIII	GCCGAG	1 cut(s)	1144
NmuCI	GTSAC	7 cut(s)	258, 344, 866, 915, 2111, 3272, 4384
NsiI	ATGCAT	1 cut(s)	2830
NspI	RCATGY	6 cut(s)	934, 1045, 1972, 2402, 3491, 3891
OliI	CACNNNNGTG	1 cut(s)	1679
PaeI	GCATGC	1 cut(s)	2402
PaeR7I	CTCGAG	1 cut(s)	3416
PciI	ACATGT	1 cut(s)	3487
PciSI	GCTCTTC	2 cut(s)	1374, 2307
PcsI	WCGNNNNNNNCGW	5 cut(s)	24, 33, 93, 311, 3240
PctI	GAATGC	4 cut(s)	757, 1516, 2830, 3174
PdiI	GCCGGC	1 cut(s)	447
PdmI	GAANNNNTTC	3 cut(s)	3360, 3817, 4413
PflMI	CCANNNNNTGG	2 cut(s)	3751, 4300
PfoI	TCCNGGA	1 cut(s)	1320
PleI	GAGTC	9 cut(s)	863, 1118, 1682, 1710, 2152, 2274, 3035, 3767, 3880
PpsI	GAGTC	9 cut(s)	863, 1118, 1682, 1710, 2152, 2274, 3035, 3767, 3880
PpuMI	RGGWCCY	2 cut(s)	1427, 2576
PscI	ACATGT	1 cut(s)	3487
PsiI	TTATAA	1 cut(s)	2954
Psp124BI	GAGCTC	1 cut(s)	1945
Psp5II	RGGWCCY	2 cut(s)	1427, 2576
Psp6I	CCWGG	5 cut(s)	191, 1705, 2053, 2264, 2755
PspFI	CCCAGC	2 cut(s)	1465, 2164
PspGI	CCWGG	5 cut(s)	191, 1705, 2053, 2264, 2755
PspPPI	RGGWCCY	2 cut(s)	1427, 2576
PstI	CTGCAG	2 cut(s)	1285, 1786
PsuI	RGATCY	7 cut(s)	775, 1210, 1324, 1342, 2973, 2992, 4453
PvuII	CAGCTG	3 cut(s)	2164, 2320, 4447
RseI	CAYNNNNRTG	4 cut(s)	1679, 2101, 2295, 3630
SacI	GAGCTC	1 cut(s)	1945
SacII	CCGCGG	2 cut(s)	148, 239
SalI	GTCGAC	1 cut(s)	26
SapI	GCTCTTC	2 cut(s)	1374, 2307
SbfI	CCTGCAGG	1 cut(s)	1285
ScaI	AGTACT	1 cut(s)	2986
SchI	GAGTC	9 cut(s)	863, 1118, 1682, 1711, 2152, 2274, 3035, 3767, 3881
ScrFI	CCNGG	8 cut(s)	193, 829, 1322, 1707, 2055, 2266, 2757, 3604
SdaI	CCTGCAGG	1 cut(s)	1285
SduI	GDGCHC	9 cut(s)	442, 675, 974, 1369, 1945, 2302, 2374, 3584, 3743
SfcI	CTRYAG	4 cut(s)	1281, 1554, 1782, 4114
Sfr274I	CTCGAG	1 cut(s)	3416
Sfr303I	CCGCGG	2 cut(s)	148, 239
SgrBI	CCGCGG	2 cut(s)	148, 239
SinI	GGWCC	6 cut(s)	138, 959, 1427, 1974, 2576, 4420
SlaI	CTCGAG	1 cut(s)	3416
SmiMI	CAYNNNNRTG	4 cut(s)	1679, 2101, 2295, 3630
SmlI	CTYRAG	9 cut(s)	1126, 1566, 1816, 1991, 2837, 2920, 3179, 3313, 3416
SmoI	CTYRAG	9 cut(s)	1126, 1566, 1816, 1991, 2837, 2920, 3179, 3313, 3416
SphI	GCATGC	1 cut(s)	2402
Sse8387I	CCTGCAGG	1 cut(s)	1285
SspMI	CTAG	7 cut(s)	104, 1373, 2205, 2745, 3621, 3894, 4341
SstI	GAGCTC	1 cut(s)	1945
StyD4I	CCNGG	8 cut(s)	191, 827, 1320, 1705, 2053, 2264, 2755, 3602
StyI	CCWWGG	2 cut(s)	103, 2359
TaiI	ACGT	6 cut(s)	161, 317, 2317, 3271, 3619, 4133
TaqI	TCGA	7 cut(s)	27, 87, 305, 3243, 3417, 3791, 4407
TaqII	GACCGA	1 cut(s)	704
TatI	WGTACW	6 cut(s)	1297, 2984, 3159, 4110, 4251, 4269
TscAI	CASTG	5 cut(s)	327, 924, 979, 1837, 2946
TseFI	GTSAC	7 cut(s)	258, 344, 866, 915, 2111, 3272, 4384
Tsp45I	GTSAC	7 cut(s)	258, 344, 866, 915, 2111, 3272, 4384
TspGWI	ACGGA	5 cut(s)	445, 1724, 2485, 3053, 4271
TspRI	CASTG	5 cut(s)	327, 924, 979, 1837, 2946
Van91I	CCANNNNNTGG	2 cut(s)	3751, 4300
Vha464I	CTTAAG	1 cut(s)	3313
VpaK11BI	GGWCC	6 cut(s)	138, 959, 1427, 1974, 2576, 4420
XapI	RAATTY	8 cut(s)	1450, 1493, 1792, 2713, 2782, 3321, 3793, 4371
XceI	RCATGY	6 cut(s)	934, 1045, 1972, 2402, 3491, 3891
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1968
XhoI	CTCGAG	1 cut(s)	3416
XmaJI	CCTAGG	1 cut(s)	103
XmiI	GTMKAC	1 cut(s)	27
XmnI	GAANNNNTTC	3 cut(s)	3360, 3817, 4413
XspI	CTAG	7 cut(s)	104, 1373, 2205, 2745, 3621, 3894, 4341
ZraI	GACGTC	1 cut(s)	159
ZrmI	AGTACT	1 cut(s)	2986
Zsp2I	ATGCAT	1 cut(s)	2830

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.