DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 609 |
| Acc36I | ACCTGC | 2 cut(s) | 208, 609 |
| AclWI | GGATC | 3 cut(s) | 230, 243, 601 |
| AcsI | RAATTY | 2 cut(s) | 305, 654 |
| AfaI | GTAC | 1 cut(s) | 225 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 170 |
| AgsI | TTSAA | 1 cut(s) | 406 |
| AleI | CACNNNNGTG | 1 cut(s) | 491 |
| AluBI | AGCT | 4 cut(s) | 112, 297, 350, 553 |
| AluI | AGCT | 4 cut(s) | 112, 297, 350, 553 |
| Alw26I | GTCTC | 2 cut(s) | 349, 451 |
| AlwI | GGATC | 3 cut(s) | 230, 243, 601 |
| AoxI | GGCC | 1 cut(s) | 474 |
| ApeKI | GCWGC | 1 cut(s) | 284 |
| ApoI | RAATTY | 2 cut(s) | 305, 654 |
| AsuC2I | CCSGG | 1 cut(s) | 24 |
| AsuHPI | GGTGA | 1 cut(s) | 325 |
| AsuNHI | GCTAGC | 1 cut(s) | 176 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 143, 176 |
| BamHI | GGATCC | 1 cut(s) | 235 |
| BbvI | GCAGC | 1 cut(s) | 296 |
| BccI | CCATC | 1 cut(s) | 551 |
| BciVI | GTATCC | 1 cut(s) | 171 |
| BcnI | CCSGG | 1 cut(s) | 24 |
| BcoDI | GTCTC | 2 cut(s) | 349, 451 |
| BfaI | CTAG | 1 cut(s) | 177 |
| BfmI | CTRYAG | 1 cut(s) | 366 |
| BfuAI | ACCTGC | 2 cut(s) | 208, 609 |
| BfuI | GTATCC | 1 cut(s) | 171 |
| BisI | GCNGC | 1 cut(s) | 285 |
| BlpI | GCTNAGC | 1 cut(s) | 527 |
| BlsI | GCNGC | 1 cut(s) | 286 |
| Bme1390I | CCNGG | 1 cut(s) | 24 |
| BmiI | GGNNCC | 1 cut(s) | 237 |
| BmrFI | CCNGG | 1 cut(s) | 24 |
| BmsI | GCATC | 2 cut(s) | 208, 461 |
| BmtI | GCTAGC | 1 cut(s) | 180 |
| BpmI | CTGGAG | 1 cut(s) | 266 |
| Bpu10I | CCTNAGC | 1 cut(s) | 33 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 527 |
| BpuEI | CTTGAG | 2 cut(s) | 363, 625 |
| BpuMI | CCSGG | 1 cut(s) | 24 |
| BsaI | GGTCTC | 1 cut(s) | 349 |
| BsaJI | CCNNGG | 1 cut(s) | 23 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 170 |
| Bse1I | ACTGG | 1 cut(s) | 249 |
| BseDI | CCNNGG | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 170 |
| BseMII | CTCAG | 3 cut(s) | 360, 516, 518 |
| BseNI | ACTGG | 1 cut(s) | 249 |
| BseXI | GCAGC | 1 cut(s) | 296 |
| BsgI | GTGCAG | 1 cut(s) | 225 |
| BshFI | GGCC | 1 cut(s) | 476 |
| BsiSI | CCGG | 2 cut(s) | 23, 591 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 170 |
| BsmAI | GTCTC | 2 cut(s) | 349, 451 |
| BsmI | GAATGC | 1 cut(s) | 328 |
| BsnI | GGCC | 1 cut(s) | 476 |
| Bso31I | GGTCTC | 1 cut(s) | 349 |
| Bsp1407I | TGTACA | 1 cut(s) | 223 |
| Bsp143I | GATC | 4 cut(s) | 228, 235, 315, 593 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 527 |
| BspANI | GGCC | 1 cut(s) | 476 |
| BspCNI | CTCAG | 3 cut(s) | 359, 515, 519 |
| BspLI | GGNNCC | 1 cut(s) | 237 |
| BspMI | ACCTGC | 2 cut(s) | 208, 609 |
| BspOI | GCTAGC | 1 cut(s) | 180 |
| BspPI | GGATC | 3 cut(s) | 230, 243, 601 |
| BspTNI | GGTCTC | 1 cut(s) | 349 |
| BsrGI | TGTACA | 1 cut(s) | 223 |
| BsrI | ACTGG | 1 cut(s) | 249 |
| BssECI | CCNNGG | 1 cut(s) | 23 |
| BssMI | GATC | 4 cut(s) | 228, 235, 315, 593 |
| Bst4CI | ACNGT | 3 cut(s) | 104, 367, 541 |
| Bst6I | CTCTTC | 1 cut(s) | 506 |
| BstAUI | TGTACA | 1 cut(s) | 223 |
| BstC8I | GCNNGC | 1 cut(s) | 178 |
| BstDEI | CTNAG | 5 cut(s) | 33, 47, 346, 502, 527 |
| BstKTI | GATC | 4 cut(s) | 231, 238, 318, 596 |
| BstMAI | GTCTC | 2 cut(s) | 349, 451 |
| BstMBI | GATC | 4 cut(s) | 228, 235, 315, 593 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 615, 632 |
| BstSCI | CCNGG | 1 cut(s) | 22 |
| BstSFI | CTRYAG | 1 cut(s) | 366 |
| BstV1I | GCAGC | 1 cut(s) | 296 |
| BstX2I | RGATCY | 1 cut(s) | 235 |
| BstYI | RGATCY | 1 cut(s) | 235 |
| BsuI | GTATCC | 1 cut(s) | 171 |
| BsuRI | GGCC | 1 cut(s) | 476 |
| BtsIMutI | CAGTG | 2 cut(s) | 175, 242 |
| BveI | ACCTGC | 2 cut(s) | 208, 609 |
| Cac8I | GCNNGC | 1 cut(s) | 178 |
| CseI | GACGC | 1 cut(s) | 169 |
| Csp6I | GTAC | 1 cut(s) | 224 |
| CviAII | CATG | 1 cut(s) | 232 |
| CviJI | RGCY | 9 cut(s) | 32, 112, 297, 350, 476, 531, 553, 590, 626 |
| CviKI_1 | RGCY | 9 cut(s) | 32, 112, 297, 350, 476, 531, 553, 590, 626 |
| CviQI | GTAC | 1 cut(s) | 224 |
| DdeI | CTNAG | 5 cut(s) | 33, 47, 346, 502, 527 |
| DpnI | GATC | 4 cut(s) | 230, 237, 317, 595 |
| DpnII | GATC | 4 cut(s) | 228, 235, 315, 593 |
| Eam1104I | CTCTTC | 1 cut(s) | 506 |
| EarI | CTCTTC | 1 cut(s) | 506 |
| Eco31I | GGTCTC | 1 cut(s) | 349 |
| FaeI | CATG | 1 cut(s) | 235 |
| FaiI | YATR | 8 cut(s) | 67, 123, 131, 233, 338, 524, 566, 575 |
| FatI | CATG | 1 cut(s) | 231 |
| Fnu4HI | GCNGC | 1 cut(s) | 285 |
| Fsp4HI | GCNGC | 1 cut(s) | 285 |
| FspBI | CTAG | 1 cut(s) | 177 |
| GluI | GCNGC | 1 cut(s) | 285 |
| GsuI | CTGGAG | 1 cut(s) | 266 |
| HaeIII | GGCC | 1 cut(s) | 476 |
| HapII | CCGG | 2 cut(s) | 23, 591 |
| HgaI | GACGC | 1 cut(s) | 169 |
| Hin1II | CATG | 1 cut(s) | 235 |
| HincII | GTYRAC | 1 cut(s) | 436 |
| HindII | GTYRAC | 1 cut(s) | 436 |
| HindIII | AAGCTT | 1 cut(s) | 551 |
| HinfI | GANTC | 3 cut(s) | 19, 500, 514 |
| HpaI | GTTAAC | 1 cut(s) | 436 |
| HpaII | CCGG | 2 cut(s) | 23, 591 |
| HphI | GGTGA | 1 cut(s) | 325 |
| Hpy166II | GTNNAC | 2 cut(s) | 436, 582 |
| Hpy188I | TCNGA | 4 cut(s) | 97, 204, 330, 505 |
| Hpy188III | TCNNGA | 2 cut(s) | 319, 604 |
| Hpy8I | GTNNAC | 2 cut(s) | 436, 582 |
| HpyAV | CCTTC | 2 cut(s) | 259, 490 |
| HpyCH4III | ACNGT | 3 cut(s) | 104, 367, 541 |
| HpyCH4V | TGCA | 5 cut(s) | 148, 242, 303, 395, 452 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 615, 632 |
| HpyF3I | CTNAG | 5 cut(s) | 33, 47, 346, 502, 527 |
| Hsp92II | CATG | 1 cut(s) | 235 |
| KspAI | GTTAAC | 1 cut(s) | 436 |
| Kzo9I | GATC | 4 cut(s) | 228, 235, 315, 593 |
| LmnI | GCTCC | 2 cut(s) | 214, 331 |
| Lsp1109I | GCAGC | 1 cut(s) | 296 |
| LweI | GCATC | 2 cut(s) | 208, 461 |
| MaeI | CTAG | 1 cut(s) | 177 |
| MaeIII | GTNAC | 2 cut(s) | 388, 467 |
| MalI | GATC | 4 cut(s) | 230, 237, 317, 595 |
| MboI | GATC | 4 cut(s) | 228, 235, 315, 593 |
| MboII | GAAGA | 1 cut(s) | 523 |
| MfeI | CAATTG | 1 cut(s) | 288 |
| MflI | RGATCY | 1 cut(s) | 235 |
| MluCI | AATT | 4 cut(s) | 288, 305, 441, 654 |
| MlyI | GAGTC | 2 cut(s) | 494, 523 |
| MnlI | CCTC | 5 cut(s) | 241, 262, 304, 387, 607 |
| MseI | TTAA | 2 cut(s) | 435, 658 |
| MslI | CAYNNNNRTG | 2 cut(s) | 278, 491 |
| MspI | CCGG | 2 cut(s) | 23, 591 |
| MspR9I | CCNGG | 1 cut(s) | 24 |
| MunI | CAATTG | 1 cut(s) | 288 |
| Mva1269I | GAATGC | 1 cut(s) | 328 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 615, 632 |
| NciI | CCSGG | 1 cut(s) | 24 |
| NdeII | GATC | 4 cut(s) | 228, 235, 315, 593 |
| NheI | GCTAGC | 1 cut(s) | 176 |
| NlaIII | CATG | 1 cut(s) | 235 |
| NlaIV | GGNNCC | 1 cut(s) | 237 |
| OliI | CACNNNNGTG | 1 cut(s) | 491 |
| PaqCI | CACCTGC | 1 cut(s) | 609 |
| PctI | GAATGC | 1 cut(s) | 328 |
| PfeI | GAWTC | 1 cut(s) | 19 |
| PkrI | GCNGC | 1 cut(s) | 286 |
| PleI | GAGTC | 2 cut(s) | 494, 522 |
| PpsI | GAGTC | 2 cut(s) | 494, 522 |
| PspN4I | GGNNCC | 1 cut(s) | 237 |
| PsuI | RGATCY | 1 cut(s) | 235 |
| RsaI | GTAC | 1 cut(s) | 225 |
| RsaNI | GTAC | 1 cut(s) | 224 |
| RseI | CAYNNNNRTG | 2 cut(s) | 278, 491 |
| SaqAI | TTAA | 2 cut(s) | 435, 658 |
| SatI | GCNGC | 1 cut(s) | 285 |
| Sau3AI | GATC | 4 cut(s) | 228, 235, 315, 593 |
| SchI | GAGTC | 2 cut(s) | 494, 523 |
| ScrFI | CCNGG | 1 cut(s) | 24 |
| SfaNI | GCATC | 2 cut(s) | 208, 461 |
| SfcI | CTRYAG | 1 cut(s) | 366 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 278, 491 |
| SmlI | CTYRAG | 2 cut(s) | 378, 604 |
| SmoI | CTYRAG | 2 cut(s) | 378, 604 |
| Sse9I | AATT | 4 cut(s) | 288, 305, 441, 654 |
| SspMI | CTAG | 1 cut(s) | 177 |
| StyD4I | CCNGG | 1 cut(s) | 22 |
| TaaI | ACNGT | 3 cut(s) | 104, 367, 541 |
| TaqI | TCGA | 1 cut(s) | 260 |
| TasI | AATT | 4 cut(s) | 288, 305, 441, 654 |
| TatI | WGTACW | 1 cut(s) | 223 |
| TfiI | GAWTC | 1 cut(s) | 19 |
| Tru1I | TTAA | 2 cut(s) | 435, 658 |
| Tru9I | TTAA | 2 cut(s) | 435, 658 |
| TscAI | CASTG | 2 cut(s) | 175, 249 |
| TseI | GCWGC | 1 cut(s) | 284 |
| TspDTI | ATGAA | 1 cut(s) | 278 |
| TspGWI | ACGGA | 1 cut(s) | 536 |
| TspRI | CASTG | 2 cut(s) | 175, 249 |
| XapI | RAATTY | 2 cut(s) | 305, 654 |
| XspI | CTAG | 1 cut(s) | 177 |