DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 233 |
| Acc65I | GGTACC | 1 cut(s) | 180 |
| AccB1I | GGYRCC | 1 cut(s) | 180 |
| AciI | CCGC | 6 cut(s) | 30, 214, 308, 341, 536, 548 |
| AclWI | GGATC | 1 cut(s) | 618 |
| AcoI | YGGCCR | 2 cut(s) | 31, 537 |
| AcsI | RAATTY | 2 cut(s) | 376, 407 |
| AcyI | GRCGYC | 1 cut(s) | 230 |
| AfaI | GTAC | 2 cut(s) | 113, 182 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 57, 219, 270 |
| AgeI | ACCGGT | 1 cut(s) | 57 |
| AjnI | CCWGG | 1 cut(s) | 263 |
| AluBI | AGCT | 1 cut(s) | 17 |
| AluI | AGCT | 1 cut(s) | 17 |
| Alw21I | GWGCWC | 1 cut(s) | 19 |
| Alw26I | GTCTC | 3 cut(s) | 447, 484, 522 |
| AlwI | GGATC | 1 cut(s) | 618 |
| AoxI | GGCC | 3 cut(s) | 31, 260, 537 |
| ApeKI | GCWGC | 1 cut(s) | 310 |
| ApoI | RAATTY | 2 cut(s) | 376, 407 |
| AsiGI | ACCGGT | 1 cut(s) | 57 |
| Asp718I | GGTACC | 1 cut(s) | 180 |
| AspA2I | CCTAGG | 1 cut(s) | 175 |
| AspLEI | GCGC | 1 cut(s) | 9 |
| AspS9I | GGNCC | 2 cut(s) | 210, 261 |
| AsuC2I | CCSGG | 1 cut(s) | 623 |
| AsuHPI | GGTGA | 3 cut(s) | 118, 466, 497 |
| AvaII | GGWCC | 1 cut(s) | 210 |
| AvrII | CCTAGG | 1 cut(s) | 175 |
| BanI | GGYRCC | 1 cut(s) | 180 |
| BanII | GRGCYC | 1 cut(s) | 19 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 71, 103 |
| BbsI | GAAGAC | 1 cut(s) | 281 |
| Bbv12I | GWGCWC | 1 cut(s) | 19 |
| BbvI | GCAGC | 1 cut(s) | 322 |
| BccI | CCATC | 6 cut(s) | 67, 185, 366, 367, 453, 482 |
| BceAI | ACGGC | 3 cut(s) | 201, 265, 354 |
| BciT130I | CCWGG | 1 cut(s) | 265 |
| BcnI | CCSGG | 1 cut(s) | 623 |
| BcoDI | GTCTC | 3 cut(s) | 447, 484, 522 |
| BfaI | CTAG | 1 cut(s) | 176 |
| BfoI | RGCGCY | 1 cut(s) | 10 |
| BisI | GCNGC | 5 cut(s) | 31, 308, 311, 537, 549 |
| BlnI | CCTAGG | 1 cut(s) | 175 |
| BlsI | GCNGC | 5 cut(s) | 32, 309, 312, 538, 550 |
| Bme1390I | CCNGG | 2 cut(s) | 265, 623 |
| Bme18I | GGWCC | 1 cut(s) | 210 |
| BmgT120I | GGNCC | 2 cut(s) | 210, 261 |
| BmiI | GGNNCC | 4 cut(s) | 62, 182, 212, 306 |
| BmrFI | CCNGG | 2 cut(s) | 265, 623 |
| BmsI | GCATC | 2 cut(s) | 396, 535 |
| BpiI | GAAGAC | 1 cut(s) | 281 |
| BpmI | CTGGAG | 1 cut(s) | 5 |
| BpuMI | CCSGG | 1 cut(s) | 623 |
| BsaHI | GRCGYC | 1 cut(s) | 230 |
| BsaI | GGTCTC | 3 cut(s) | 447, 484, 522 |
| BsaJI | CCNNGG | 5 cut(s) | 175, 217, 263, 412, 622 |
| BsaWI | WCCGGW | 2 cut(s) | 57, 482 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 427, 457 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 57, 219, 270 |
| Bse118I | RCCGGY | 1 cut(s) | 57 |
| Bse1I | ACTGG | 1 cut(s) | 540 |
| Bse3DI | GCAATG | 1 cut(s) | 357 |
| BseBI | CCWGG | 1 cut(s) | 265 |
| BseDI | CCNNGG | 5 cut(s) | 175, 217, 263, 412, 622 |
| BseGI | GGATG | 1 cut(s) | 244 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 57, 219, 270 |
| BseMI | GCAATG | 1 cut(s) | 357 |
| BseNI | ACTGG | 1 cut(s) | 540 |
| BseRI | GAGGAG | 2 cut(s) | 155, 283 |
| BseXI | GCAGC | 1 cut(s) | 322 |
| Bsh1285I | CGRYCG | 1 cut(s) | 58 |
| BshFI | GGCC | 3 cut(s) | 33, 262, 539 |
| BshNI | GGYRCC | 1 cut(s) | 180 |
| BshTI | ACCGGT | 1 cut(s) | 57 |
| BsiEI | CGRYCG | 1 cut(s) | 58 |
| BsiHKAI | GWGCWC | 1 cut(s) | 19 |
| BsiSI | CCGG | 3 cut(s) | 58, 483, 622 |
| BslFI | GGGAC | 2 cut(s) | 164, 448 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 57, 219, 270 |
| BsmAI | GTCTC | 3 cut(s) | 447, 484, 522 |
| BsmFI | GGGAC | 2 cut(s) | 164, 448 |
| BsnI | GGCC | 3 cut(s) | 33, 262, 539 |
| Bso31I | GGTCTC | 3 cut(s) | 447, 484, 522 |
| Bsp1286I | GDGCHC | 1 cut(s) | 19 |
| Bsp143I | GATC | 1 cut(s) | 610 |
| BspACI | CCGC | 6 cut(s) | 30, 214, 308, 341, 536, 548 |
| BspANI | GGCC | 3 cut(s) | 33, 262, 539 |
| BspLI | GGNNCC | 4 cut(s) | 62, 182, 212, 306 |
| BspPI | GGATC | 1 cut(s) | 618 |
| BspT107I | GGYRCC | 1 cut(s) | 180 |
| BspTNI | GGTCTC | 3 cut(s) | 447, 484, 522 |
| BsrDI | GCAATG | 1 cut(s) | 357 |
| BsrFI | RCCGGY | 1 cut(s) | 57 |
| BsrI | ACTGG | 1 cut(s) | 540 |
| BssAI | RCCGGY | 1 cut(s) | 57 |
| BssECI | CCNNGG | 5 cut(s) | 175, 217, 263, 412, 622 |
| BssMI | GATC | 1 cut(s) | 610 |
| BssNI | GRCGYC | 1 cut(s) | 230 |
| BssT1I | CCWWGG | 1 cut(s) | 175 |
| Bst2UI | CCWGG | 1 cut(s) | 265 |
| Bst4CI | ACNGT | 3 cut(s) | 97, 280, 533 |
| Bst6I | CTCTTC | 3 cut(s) | 195, 493, 504 |
| BstACI | GRCGYC | 1 cut(s) | 230 |
| BstDSI | CCRYGG | 1 cut(s) | 217 |
| BstF5I | GGATG | 1 cut(s) | 244 |
| BstH2I | RGCGCY | 1 cut(s) | 10 |
| BstHHI | GCGC | 1 cut(s) | 9 |
| BstKTI | GATC | 1 cut(s) | 613 |
| BstMAI | GTCTC | 3 cut(s) | 447, 484, 522 |
| BstMBI | GATC | 1 cut(s) | 610 |
| BstMCI | CGRYCG | 1 cut(s) | 58 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 319, 347, 545, 557 |
| BstNI | CCWGG | 1 cut(s) | 265 |
| BstSCI | CCNGG | 2 cut(s) | 263, 621 |
| BstV1I | GCAGC | 1 cut(s) | 322 |
| BstV2I | GAAGAC | 1 cut(s) | 281 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 191 |
| BsuRI | GGCC | 3 cut(s) | 33, 262, 539 |
| BtgI | CCRYGG | 1 cut(s) | 217 |
| BtsCI | GGATG | 1 cut(s) | 244 |
| BtsIMutI | CAGTG | 1 cut(s) | 547 |
| CfoI | GCGC | 1 cut(s) | 9 |
| Cfr10I | RCCGGY | 1 cut(s) | 57 |
| Cfr13I | GGNCC | 2 cut(s) | 210, 261 |
| CseI | GACGC | 1 cut(s) | 125 |
| Csp6I | GTAC | 2 cut(s) | 112, 181 |
| CspAI | ACCGGT | 1 cut(s) | 57 |
| CviQI | GTAC | 2 cut(s) | 112, 181 |
| DpnI | GATC | 1 cut(s) | 612 |
| DpnII | GATC | 1 cut(s) | 610 |
| EaeI | YGGCCR | 2 cut(s) | 31, 537 |
| Eam1104I | CTCTTC | 3 cut(s) | 195, 493, 504 |
| EarI | CTCTTC | 3 cut(s) | 195, 493, 504 |
| Ecl136II | GAGCTC | 1 cut(s) | 17 |
| Eco130I | CCWWGG | 1 cut(s) | 175 |
| Eco24I | GRGCYC | 1 cut(s) | 19 |
| Eco31I | GGTCTC | 3 cut(s) | 447, 484, 522 |
| Eco47I | GGWCC | 1 cut(s) | 210 |
| Eco53kI | GAGCTC | 1 cut(s) | 17 |
| EcoICRI | GAGCTC | 1 cut(s) | 17 |
| EcoRI | GAATTC | 2 cut(s) | 376, 407 |
| EcoRII | CCWGG | 1 cut(s) | 263 |
| EcoT14I | CCWWGG | 1 cut(s) | 175 |
| EcoT38I | GRGCYC | 1 cut(s) | 19 |
| ErhI | CCWWGG | 1 cut(s) | 175 |
| FaiI | YATR | 3 cut(s) | 257, 397, 597 |
| FaqI | GGGAC | 2 cut(s) | 164, 448 |
| FauI | CCCGC | 1 cut(s) | 221 |
| Fnu4HI | GCNGC | 5 cut(s) | 31, 308, 311, 537, 549 |
| FokI | GGATG | 1 cut(s) | 251 |
| FriOI | GRGCYC | 1 cut(s) | 19 |
| Fsp4HI | GCNGC | 5 cut(s) | 31, 308, 311, 537, 549 |
| FspBI | CTAG | 1 cut(s) | 176 |
| GlaI | GCGC | 1 cut(s) | 8 |
| GluI | GCNGC | 5 cut(s) | 31, 308, 311, 537, 549 |
| GsuI | CTGGAG | 1 cut(s) | 5 |
| HaeII | RGCGCY | 1 cut(s) | 10 |
| HaeIII | GGCC | 3 cut(s) | 33, 262, 539 |
| HapII | CCGG | 3 cut(s) | 58, 483, 622 |
| HgaI | GACGC | 1 cut(s) | 125 |
| HhaI | GCGC | 1 cut(s) | 9 |
| Hin1I | GRCGYC | 1 cut(s) | 230 |
| Hin6I | GCGC | 1 cut(s) | 7 |
| HinP1I | GCGC | 1 cut(s) | 7 |
| HincII | GTYRAC | 1 cut(s) | 100 |
| HindII | GTYRAC | 1 cut(s) | 100 |
| HinfI | GANTC | 2 cut(s) | 242, 355 |
| HpaII | CCGG | 3 cut(s) | 58, 483, 622 |
| HphI | GGTGA | 3 cut(s) | 118, 466, 497 |
| Hpy166II | GTNNAC | 1 cut(s) | 100 |
| Hpy188I | TCNGA | 5 cut(s) | 43, 228, 238, 247, 469 |
| Hpy188III | TCNNGA | 1 cut(s) | 64 |
| Hpy8I | GTNNAC | 1 cut(s) | 100 |
| Hpy99I | CGWCG | 8 cut(s) | 56, 119, 170, 191, 232, 235, 238, 334 |
| HpyAV | CCTTC | 4 cut(s) | 20, 283, 289, 295 |
| HpyCH4III | ACNGT | 3 cut(s) | 97, 280, 533 |
| HpyCH4IV | ACGT | 1 cut(s) | 230 |
| HpyCH4V | TGCA | 1 cut(s) | 578 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 319, 347, 545, 557 |
| HpySE526I | ACGT | 1 cut(s) | 230 |
| Hsp92I | GRCGYC | 1 cut(s) | 230 |
| HspAI | GCGC | 1 cut(s) | 7 |
| KpnI | GGTACC | 1 cut(s) | 184 |
| Kzo9I | GATC | 1 cut(s) | 610 |
| LmnI | GCTCC | 2 cut(s) | 4, 304 |
| Lsp1109I | GCAGC | 1 cut(s) | 322 |
| LweI | GCATC | 2 cut(s) | 396, 535 |
| MaeI | CTAG | 1 cut(s) | 176 |
| MaeII | ACGT | 1 cut(s) | 230 |
| MalI | GATC | 1 cut(s) | 612 |
| MboI | GATC | 1 cut(s) | 610 |
| MboII | GAAGA | 8 cut(s) | 212, 286, 298, 372, 510, 515, 518, 521 |
| MhlI | GDGCHC | 1 cut(s) | 19 |
| MluCI | AATT | 5 cut(s) | 325, 376, 401, 407, 566 |
| MmeI | TCCRAC | 3 cut(s) | 216, 251, 447 |
| MseI | TTAA | 2 cut(s) | 368, 570 |
| MslI | CAYNNNNRTG | 1 cut(s) | 189 |
| MspI | CCGG | 3 cut(s) | 58, 483, 622 |
| MspR9I | CCNGG | 2 cut(s) | 265, 623 |
| MvaI | CCWGG | 1 cut(s) | 265 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 319, 347, 545, 557 |
| NciI | CCSGG | 1 cut(s) | 623 |
| NdeII | GATC | 1 cut(s) | 610 |
| NlaIV | GGNNCC | 4 cut(s) | 62, 182, 212, 306 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 165, 192 |
| PfeI | GAWTC | 2 cut(s) | 242, 355 |
| PflFI | GACNNNGTC | 2 cut(s) | 232, 439 |
| PinAI | ACCGGT | 1 cut(s) | 57 |
| PkrI | GCNGC | 5 cut(s) | 32, 309, 312, 538, 550 |
| Psp124BI | GAGCTC | 1 cut(s) | 19 |
| Psp6I | CCWGG | 1 cut(s) | 263 |
| PspGI | CCWGG | 1 cut(s) | 263 |
| PspN4I | GGNNCC | 4 cut(s) | 62, 182, 212, 306 |
| PspPI | GGNCC | 2 cut(s) | 210, 261 |
| PsyI | GACNNNGTC | 2 cut(s) | 232, 439 |
| RsaI | GTAC | 2 cut(s) | 113, 182 |
| RsaNI | GTAC | 2 cut(s) | 112, 181 |
| RseI | CAYNNNNRTG | 1 cut(s) | 189 |
| SacI | GAGCTC | 1 cut(s) | 19 |
| SaqAI | TTAA | 2 cut(s) | 368, 570 |
| SatI | GCNGC | 5 cut(s) | 31, 308, 311, 537, 549 |
| Sau3AI | GATC | 1 cut(s) | 610 |
| Sau96I | GGNCC | 2 cut(s) | 210, 261 |
| ScrFI | CCNGG | 2 cut(s) | 265, 623 |
| SduI | GDGCHC | 1 cut(s) | 19 |
| SetI | ASST | 6 cut(s) | 19, 39, 137, 177, 233, 518 |
| SfaNI | GCATC | 2 cut(s) | 396, 535 |
| SinI | GGWCC | 1 cut(s) | 210 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 189 |
| Sse9I | AATT | 5 cut(s) | 325, 376, 401, 407, 566 |
| SsiI | CCGC | 6 cut(s) | 30, 214, 308, 341, 536, 548 |
| SspMI | CTAG | 1 cut(s) | 176 |
| SstI | GAGCTC | 1 cut(s) | 19 |
| StyD4I | CCNGG | 2 cut(s) | 263, 621 |
| StyI | CCWWGG | 1 cut(s) | 175 |
| TaaI | ACNGT | 3 cut(s) | 97, 280, 533 |
| TaiI | ACGT | 1 cut(s) | 233 |
| TaqI | TCGA | 4 cut(s) | 13, 159, 332, 518 |
| TaqII | GACCGA | 1 cut(s) | 404 |
| TasI | AATT | 5 cut(s) | 325, 376, 401, 407, 566 |
| TauI | GCSGC | 4 cut(s) | 33, 310, 539, 551 |
| TfiI | GAWTC | 2 cut(s) | 242, 355 |
| Tru1I | TTAA | 2 cut(s) | 368, 570 |
| Tru9I | TTAA | 2 cut(s) | 368, 570 |
| TscAI | CASTG | 1 cut(s) | 547 |
| TseI | GCWGC | 1 cut(s) | 310 |
| TspDTI | ATGAA | 2 cut(s) | 145, 255 |
| TspGWI | ACGGA | 1 cut(s) | 354 |
| TspRI | CASTG | 1 cut(s) | 547 |
| Tth111I | GACNNNGTC | 2 cut(s) | 232, 439 |
| VpaK11BI | GGWCC | 1 cut(s) | 210 |
| XapI | RAATTY | 2 cut(s) | 376, 407 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 435 |
| XmaJI | CCTAGG | 1 cut(s) | 175 |
| XspI | CTAG | 1 cut(s) | 176 |
| ZraI | GACGTC | 1 cut(s) | 231 |