DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 61 |
| AciI | CCGC | 3 cut(s) | 59, 92, 294 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 15, 91 |
| AjiI | CACGTC | 1 cut(s) | 138 |
| AjnI | CCWGG | 1 cut(s) | 8 |
| Alw26I | GTCTC | 1 cut(s) | 244 |
| AoxI | GGCC | 2 cut(s) | 5, 174 |
| AspS9I | GGNCC | 1 cut(s) | 6 |
| AsuHPI | GGTGA | 1 cut(s) | 179 |
| BaeGI | GKGCMC | 1 cut(s) | 265 |
| BbsI | GAAGAC | 1 cut(s) | 26 |
| BccI | CCATC | 2 cut(s) | 100, 158 |
| BceAI | ACGGC | 1 cut(s) | 105 |
| BciT130I | CCWGG | 1 cut(s) | 10 |
| BcoDI | GTCTC | 1 cut(s) | 244 |
| BisI | GCNGC | 2 cut(s) | 59, 93 |
| BlsI | GCNGC | 2 cut(s) | 60, 94 |
| Bme1390I | CCNGG | 1 cut(s) | 10 |
| BmgBI | CACGTC | 1 cut(s) | 138 |
| BmgT120I | GGNCC | 1 cut(s) | 6 |
| BmiI | GGNNCC | 1 cut(s) | 57 |
| BmrFI | CCNGG | 1 cut(s) | 10 |
| BpiI | GAAGAC | 1 cut(s) | 26 |
| BsaJI | CCNNGG | 1 cut(s) | 8 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 15, 91 |
| Bse118I | RCCGGY | 1 cut(s) | 268 |
| BseBI | CCWGG | 1 cut(s) | 10 |
| BseDI | CCNNGG | 1 cut(s) | 8 |
| BseGI | GGATG | 3 cut(s) | 169, 211, 331 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 15, 91 |
| BseRI | GAGGAG | 4 cut(s) | 28, 271, 289, 335 |
| BseSI | GKGCMC | 1 cut(s) | 265 |
| BsgI | GTGCAG | 1 cut(s) | 323 |
| Bsh1236I | CGCG | 1 cut(s) | 61 |
| BshFI | GGCC | 2 cut(s) | 7, 176 |
| BsiSI | CCGG | 2 cut(s) | 230, 269 |
| BslFI | GGGAC | 1 cut(s) | 67 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 15, 91 |
| BsmAI | GTCTC | 1 cut(s) | 244 |
| BsmBI | CGTCTC | 1 cut(s) | 244 |
| BsmFI | GGGAC | 1 cut(s) | 67 |
| BsnI | GGCC | 2 cut(s) | 7, 176 |
| Bsp1286I | GDGCHC | 1 cut(s) | 265 |
| BspACI | CCGC | 3 cut(s) | 59, 92, 294 |
| BspANI | GGCC | 2 cut(s) | 7, 176 |
| BspFNI | CGCG | 1 cut(s) | 61 |
| BspLI | GGNNCC | 1 cut(s) | 57 |
| BsrFI | RCCGGY | 1 cut(s) | 268 |
| BssAI | RCCGGY | 1 cut(s) | 268 |
| BssECI | CCNNGG | 1 cut(s) | 8 |
| Bst2UI | CCWGG | 1 cut(s) | 10 |
| Bst4CI | ACNGT | 1 cut(s) | 25 |
| Bst6I | CTCTTC | 1 cut(s) | 299 |
| BstC8I | GCNNGC | 1 cut(s) | 270 |
| BstF5I | GGATG | 3 cut(s) | 169, 211, 331 |
| BstFNI | CGCG | 1 cut(s) | 61 |
| BstMAI | GTCTC | 1 cut(s) | 244 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 98, 269 |
| BstNI | CCWGG | 1 cut(s) | 10 |
| BstSCI | CCNGG | 1 cut(s) | 8 |
| BstSLI | GKGCMC | 1 cut(s) | 265 |
| BstUI | CGCG | 1 cut(s) | 61 |
| BstV2I | GAAGAC | 1 cut(s) | 26 |
| BsuRI | GGCC | 2 cut(s) | 7, 176 |
| BtgZI | GCGATG | 1 cut(s) | 116 |
| BtrI | CACGTC | 1 cut(s) | 138 |
| BtsCI | GGATG | 3 cut(s) | 169, 211, 331 |
| Cac8I | GCNNGC | 1 cut(s) | 270 |
| Cfr10I | RCCGGY | 1 cut(s) | 268 |
| Cfr13I | GGNCC | 1 cut(s) | 6 |
| CviJI | RGCY | 9 cut(s) | 7, 58, 73, 95, 121, 176, 229, 239, 268 |
| CviKI_1 | RGCY | 9 cut(s) | 7, 58, 73, 95, 121, 176, 229, 239, 268 |
| Eam1104I | CTCTTC | 1 cut(s) | 299 |
| EarI | CTCTTC | 1 cut(s) | 299 |
| EcoRII | CCWGG | 1 cut(s) | 8 |
| Esp3I | CGTCTC | 1 cut(s) | 244 |
| FaiI | YATR | 2 cut(s) | 161, 203 |
| FaqI | GGGAC | 1 cut(s) | 67 |
| Fnu4HI | GCNGC | 2 cut(s) | 59, 93 |
| FokI | GGATG | 3 cut(s) | 176, 218, 338 |
| Fsp4HI | GCNGC | 2 cut(s) | 59, 93 |
| GluI | GCNGC | 2 cut(s) | 59, 93 |
| HaeIII | GGCC | 2 cut(s) | 7, 176 |
| HapII | CCGG | 2 cut(s) | 230, 269 |
| HpaII | CCGG | 2 cut(s) | 230, 269 |
| HphI | GGTGA | 1 cut(s) | 179 |
| Hpy166II | GTNNAC | 1 cut(s) | 135 |
| Hpy188I | TCNGA | 1 cut(s) | 288 |
| Hpy188III | TCNNGA | 1 cut(s) | 301 |
| Hpy8I | GTNNAC | 1 cut(s) | 135 |
| Hpy99I | CGWCG | 3 cut(s) | 130, 142, 145 |
| HpyAV | CCTTC | 4 cut(s) | 28, 34, 40, 46 |
| HpyCH4III | ACNGT | 1 cut(s) | 25 |
| HpyCH4IV | ACGT | 1 cut(s) | 137 |
| HpyCH4V | TGCA | 1 cut(s) | 340 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 98, 269 |
| HpySE526I | ACGT | 1 cut(s) | 137 |
| KroI | GCCGGC | 1 cut(s) | 268 |
| KroNI | GCCGGC | 1 cut(s) | 270 |
| LmnI | GCTCC | 1 cut(s) | 55 |
| LpnPI | CCDG | 3 cut(s) | 22, 243, 282 |
| MaeII | ACGT | 1 cut(s) | 137 |
| MaeIII | GTNAC | 1 cut(s) | 167 |
| MboII | GAAGA | 5 cut(s) | 31, 43, 316, 341, 344 |
| MhlI | GDGCHC | 1 cut(s) | 265 |
| MluCI | AATT | 1 cut(s) | 76 |
| MroNI | GCCGGC | 1 cut(s) | 268 |
| MseI | TTAA | 2 cut(s) | 150, 346 |
| MspI | CCGG | 2 cut(s) | 230, 269 |
| MspR9I | CCNGG | 1 cut(s) | 10 |
| MvaI | CCWGG | 1 cut(s) | 10 |
| MvnI | CGCG | 1 cut(s) | 61 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 98, 269 |
| NaeI | GCCGGC | 1 cut(s) | 270 |
| NgoMIV | GCCGGC | 1 cut(s) | 268 |
| NlaIV | GGNNCC | 1 cut(s) | 57 |
| NmeAIII | GCCGAG | 1 cut(s) | 244 |
| NmuCI | GTSAC | 1 cut(s) | 167 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 134 |
| PdiI | GCCGGC | 1 cut(s) | 270 |
| PkrI | GCNGC | 2 cut(s) | 60, 94 |
| Psp6I | CCWGG | 1 cut(s) | 8 |
| PspGI | CCWGG | 1 cut(s) | 8 |
| PspN4I | GGNNCC | 1 cut(s) | 57 |
| PspPI | GGNCC | 1 cut(s) | 6 |
| SaqAI | TTAA | 2 cut(s) | 150, 346 |
| SatI | GCNGC | 2 cut(s) | 59, 93 |
| Sau96I | GGNCC | 1 cut(s) | 6 |
| ScrFI | CCNGG | 1 cut(s) | 10 |
| SduI | GDGCHC | 1 cut(s) | 265 |
| SetI | ASST | 1 cut(s) | 140 |
| Sse9I | AATT | 1 cut(s) | 76 |
| SsiI | CCGC | 3 cut(s) | 59, 92, 294 |
| StyD4I | CCNGG | 1 cut(s) | 8 |
| TaaI | ACNGT | 1 cut(s) | 25 |
| TaiI | ACGT | 1 cut(s) | 140 |
| TaqI | TCGA | 1 cut(s) | 128 |
| TasI | AATT | 1 cut(s) | 76 |
| TauI | GCSGC | 2 cut(s) | 61, 95 |
| Tru1I | TTAA | 2 cut(s) | 150, 346 |
| Tru9I | TTAA | 2 cut(s) | 150, 346 |
| TseFI | GTSAC | 1 cut(s) | 167 |
| Tsp45I | GTSAC | 1 cut(s) | 167 |
| TspDTI | ATGAA | 1 cut(s) | 342 |
| TspGWI | ACGGA | 1 cut(s) | 268 |