DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 101 |
| AciI | CCGC | 3 cut(s) | 71, 87, 209 |
| AclWI | GGATC | 1 cut(s) | 491 |
| AcyI | GRCGYC | 1 cut(s) | 98 |
| AfaI | GTAC | 1 cut(s) | 67 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 11, 138, 404 |
| AflIII | ACRYGT | 1 cut(s) | 430 |
| AjiI | CACGTC | 1 cut(s) | 442 |
| AjnI | CCWGG | 1 cut(s) | 131 |
| Alw21I | GWGCWC | 1 cut(s) | 441 |
| Alw26I | GTCTC | 1 cut(s) | 421 |
| Alw44I | GTGCAC | 1 cut(s) | 437 |
| AlwI | GGATC | 1 cut(s) | 491 |
| Ama87I | CYCGRG | 1 cut(s) | 329 |
| AoxI | GGCC | 2 cut(s) | 128, 285 |
| ApaLI | GTGCAC | 1 cut(s) | 437 |
| AspS9I | GGNCC | 1 cut(s) | 129 |
| AsuHPI | GGTGA | 1 cut(s) | 290 |
| AvaI | CYCGRG | 1 cut(s) | 329 |
| BaeGI | GKGCMC | 2 cut(s) | 376, 441 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 25, 57 |
| BbsI | GAAGAC | 1 cut(s) | 149 |
| Bbv12I | GWGCWC | 1 cut(s) | 441 |
| BccI | CCATC | 3 cut(s) | 21, 269, 357 |
| BciT130I | CCWGG | 1 cut(s) | 133 |
| BcoDI | GTCTC | 1 cut(s) | 421 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 88 |
| BisI | GCNGC | 2 cut(s) | 71, 210 |
| BlsI | GCNGC | 2 cut(s) | 72, 211 |
| Bme1390I | CCNGG | 1 cut(s) | 133 |
| BmeT110I | CYCGRG | 1 cut(s) | 329 |
| BmgBI | CACGTC | 1 cut(s) | 442 |
| BmgT120I | GGNCC | 1 cut(s) | 129 |
| BmiI | GGNNCC | 2 cut(s) | 16, 168 |
| BmrFI | CCNGG | 1 cut(s) | 133 |
| BpiI | GAAGAC | 1 cut(s) | 149 |
| Bsa29I | ATCGAT | 1 cut(s) | 486 |
| BsaHI | GRCGYC | 1 cut(s) | 98 |
| BsaJI | CCNNGG | 1 cut(s) | 131 |
| BsaWI | WCCGGW | 1 cut(s) | 404 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 11, 138, 404 |
| BseBI | CCWGG | 1 cut(s) | 133 |
| BseCI | ATCGAT | 1 cut(s) | 486 |
| BseDI | CCNNGG | 1 cut(s) | 131 |
| BseGI | GGATG | 3 cut(s) | 112, 280, 322 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 11, 138, 404 |
| BseRI | GAGGAG | 3 cut(s) | 151, 382, 400 |
| BseSI | GKGCMC | 2 cut(s) | 376, 441 |
| BshFI | GGCC | 2 cut(s) | 130, 287 |
| BshVI | ATCGAT | 1 cut(s) | 486 |
| BsiHKAI | GWGCWC | 1 cut(s) | 441 |
| BsiHKCI | CYCGRG | 1 cut(s) | 329 |
| BsiSI | CCGG | 1 cut(s) | 405 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 11, 138, 404 |
| BsmAI | GTCTC | 1 cut(s) | 421 |
| BsnI | GGCC | 2 cut(s) | 130, 287 |
| BsoBI | CYCGRG | 1 cut(s) | 329 |
| Bsp1286I | GDGCHC | 2 cut(s) | 376, 441 |
| Bsp143I | GATC | 2 cut(s) | 38, 483 |
| BspACI | CCGC | 3 cut(s) | 71, 87, 209 |
| BspANI | GGCC | 2 cut(s) | 130, 287 |
| BspDI | ATCGAT | 1 cut(s) | 486 |
| BspLI | GGNNCC | 2 cut(s) | 16, 168 |
| BspPI | GGATC | 1 cut(s) | 491 |
| BssECI | CCNNGG | 1 cut(s) | 131 |
| BssMI | GATC | 2 cut(s) | 38, 483 |
| BssNI | GRCGYC | 1 cut(s) | 98 |
| Bst2UI | CCWGG | 1 cut(s) | 133 |
| Bst4CI | ACNGT | 3 cut(s) | 51, 148, 202 |
| BstACI | GRCGYC | 1 cut(s) | 98 |
| BstC8I | GCNNGC | 1 cut(s) | 89 |
| BstF5I | GGATG | 3 cut(s) | 112, 280, 322 |
| BstKTI | GATC | 2 cut(s) | 41, 486 |
| BstMAI | GTCTC | 1 cut(s) | 421 |
| BstMBI | GATC | 2 cut(s) | 38, 483 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 79, 88, 380 |
| BstNI | CCWGG | 1 cut(s) | 133 |
| BstNSI | RCATGY | 1 cut(s) | 434 |
| BstSCI | CCNGG | 1 cut(s) | 131 |
| BstSLI | GKGCMC | 2 cut(s) | 376, 441 |
| BstV2I | GAAGAC | 1 cut(s) | 149 |
| Bsu15I | ATCGAT | 1 cut(s) | 486 |
| BsuRI | GGCC | 2 cut(s) | 130, 287 |
| BsuTUI | ATCGAT | 1 cut(s) | 486 |
| BtgZI | GCGATG | 2 cut(s) | 63, 227 |
| BtrI | CACGTC | 1 cut(s) | 442 |
| BtsCI | GGATG | 3 cut(s) | 112, 280, 322 |
| Cac8I | GCNNGC | 1 cut(s) | 89 |
| Cfr13I | GGNCC | 1 cut(s) | 129 |
| ClaI | ATCGAT | 1 cut(s) | 486 |
| Csp6I | GTAC | 1 cut(s) | 66 |
| CviAII | CATG | 1 cut(s) | 431 |
| CviQI | GTAC | 1 cut(s) | 66 |
| DpnI | GATC | 2 cut(s) | 40, 485 |
| DpnII | GATC | 2 cut(s) | 38, 483 |
| Eco88I | CYCGRG | 1 cut(s) | 329 |
| EcoRII | CCWGG | 1 cut(s) | 131 |
| FaeI | CATG | 1 cut(s) | 434 |
| FaiI | YATR | 2 cut(s) | 125, 432 |
| FatI | CATG | 1 cut(s) | 430 |
| FauI | CCCGC | 1 cut(s) | 80 |
| Fnu4HI | GCNGC | 2 cut(s) | 71, 210 |
| FokI | GGATG | 3 cut(s) | 119, 287, 329 |
| Fsp4HI | GCNGC | 2 cut(s) | 71, 210 |
| GluI | GCNGC | 2 cut(s) | 71, 210 |
| HaeIII | GGCC | 2 cut(s) | 130, 287 |
| HapII | CCGG | 1 cut(s) | 405 |
| Hin1I | GRCGYC | 1 cut(s) | 98 |
| Hin1II | CATG | 1 cut(s) | 434 |
| HincII | GTYRAC | 2 cut(s) | 9, 54 |
| HindII | GTYRAC | 2 cut(s) | 9, 54 |
| HinfI | GANTC | 2 cut(s) | 110, 325 |
| HpaII | CCGG | 1 cut(s) | 405 |
| HphI | GGTGA | 1 cut(s) | 290 |
| Hpy166II | GTNNAC | 4 cut(s) | 9, 54, 246, 439 |
| Hpy188I | TCNGA | 5 cut(s) | 96, 106, 115, 399, 470 |
| Hpy188III | TCNNGA | 2 cut(s) | 18, 329 |
| Hpy8I | GTNNAC | 4 cut(s) | 9, 54, 246, 439 |
| Hpy99I | CGWCG | 8 cut(s) | 100, 103, 106, 122, 196, 241, 256, 446 |
| HpyAV | CCTTC | 2 cut(s) | 151, 157 |
| HpyCH4III | ACNGT | 3 cut(s) | 51, 148, 202 |
| HpyCH4IV | ACGT | 2 cut(s) | 98, 441 |
| HpyCH4V | TGCA | 1 cut(s) | 439 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 79, 88, 380 |
| HpySE526I | ACGT | 2 cut(s) | 98, 441 |
| Hsp92I | GRCGYC | 1 cut(s) | 98 |
| Hsp92II | CATG | 1 cut(s) | 434 |
| Kzo9I | GATC | 2 cut(s) | 38, 483 |
| LmnI | GCTCC | 1 cut(s) | 166 |
| LpnPI | CCDG | 6 cut(s) | 31, 118, 145, 326, 418, 466 |
| MaeII | ACGT | 2 cut(s) | 98, 441 |
| MaeIII | GTNAC | 1 cut(s) | 278 |
| MalI | GATC | 2 cut(s) | 40, 485 |
| MboI | GATC | 2 cut(s) | 38, 483 |
| MboII | GAAGA | 2 cut(s) | 154, 166 |
| MhlI | GDGCHC | 2 cut(s) | 376, 441 |
| MluCI | AATT | 1 cut(s) | 187 |
| MmeI | TCCRAC | 3 cut(s) | 84, 119, 448 |
| MseI | TTAA | 2 cut(s) | 261, 411 |
| MspI | CCGG | 1 cut(s) | 405 |
| MspR9I | CCNGG | 1 cut(s) | 133 |
| MvaI | CCWGG | 1 cut(s) | 133 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 79, 88, 380 |
| NdeII | GATC | 2 cut(s) | 38, 483 |
| NlaIII | CATG | 1 cut(s) | 434 |
| NlaIV | GGNNCC | 2 cut(s) | 16, 168 |
| NmuCI | GTSAC | 1 cut(s) | 278 |
| NspI | RCATGY | 1 cut(s) | 434 |
| PciI | ACATGT | 1 cut(s) | 430 |
| PfeI | GAWTC | 2 cut(s) | 110, 325 |
| PflFI | GACNNNGTC | 1 cut(s) | 100 |
| PkrI | GCNGC | 2 cut(s) | 72, 211 |
| PscI | ACATGT | 1 cut(s) | 430 |
| Psp6I | CCWGG | 1 cut(s) | 131 |
| PspGI | CCWGG | 1 cut(s) | 131 |
| PspN4I | GGNNCC | 2 cut(s) | 16, 168 |
| PspPI | GGNCC | 1 cut(s) | 129 |
| PsyI | GACNNNGTC | 1 cut(s) | 100 |
| RsaI | GTAC | 1 cut(s) | 67 |
| RsaNI | GTAC | 1 cut(s) | 66 |
| SaqAI | TTAA | 2 cut(s) | 261, 411 |
| SatI | GCNGC | 2 cut(s) | 71, 210 |
| Sau3AI | GATC | 2 cut(s) | 38, 483 |
| Sau96I | GGNCC | 1 cut(s) | 129 |
| ScrFI | CCNGG | 1 cut(s) | 133 |
| SduI | GDGCHC | 2 cut(s) | 376, 441 |
| SetI | ASST | 3 cut(s) | 16, 101, 444 |
| Sse9I | AATT | 1 cut(s) | 187 |
| SsiI | CCGC | 3 cut(s) | 71, 87, 209 |
| StyD4I | CCNGG | 1 cut(s) | 131 |
| TaaI | ACNGT | 3 cut(s) | 51, 148, 202 |
| TaiI | ACGT | 2 cut(s) | 101, 444 |
| TaqI | TCGA | 3 cut(s) | 239, 444, 486 |
| TasI | AATT | 1 cut(s) | 187 |
| TauI | GCSGC | 2 cut(s) | 73, 212 |
| TfiI | GAWTC | 2 cut(s) | 110, 325 |
| Tru1I | TTAA | 2 cut(s) | 261, 411 |
| Tru9I | TTAA | 2 cut(s) | 261, 411 |
| TseFI | GTSAC | 1 cut(s) | 278 |
| Tsp45I | GTSAC | 1 cut(s) | 278 |
| TspDTI | ATGAA | 1 cut(s) | 123 |
| Tth111I | GACNNNGTC | 1 cut(s) | 100 |
| VneI | GTGCAC | 1 cut(s) | 437 |
| XceI | RCATGY | 1 cut(s) | 434 |
| ZraI | GACGTC | 1 cut(s) | 99 |