DNA polymerase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DNA_pol_alpha_N | PF12254 | 2 - 36 | 1.3e-08 | DNA polymerase alpha subunit p180 N terminal |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 27 |
| AccII | CGCG | 2 cut(s) | 112, 170 |
| AciI | CCGC | 6 cut(s) | 110, 168, 170, 200, 203, 206 |
| AcyI | GRCGYC | 1 cut(s) | 45 |
| AfaI | GTAC | 2 cut(s) | 41, 489 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 401 |
| AgsI | TTSAA | 1 cut(s) | 244 |
| Alw26I | GTCTC | 2 cut(s) | 113, 352 |
| AoxI | GGCC | 3 cut(s) | 100, 171, 282 |
| AspA2I | CCTAGG | 1 cut(s) | 103 |
| AspLEI | GCGC | 1 cut(s) | 337 |
| AspS9I | GGNCC | 2 cut(s) | 107, 172 |
| AsuHPI | GGTGA | 1 cut(s) | 287 |
| AvaII | GGWCC | 1 cut(s) | 107 |
| AvrII | CCTAGG | 1 cut(s) | 103 |
| BaeGI | GKGCMC | 1 cut(s) | 373 |
| BbsI | GAAGAC | 1 cut(s) | 141 |
| BccI | CCATC | 3 cut(s) | 56, 208, 266 |
| BceAI | ACGGC | 1 cut(s) | 115 |
| BclI | TGATCA | 2 cut(s) | 448, 508 |
| BcoDI | GTCTC | 2 cut(s) | 113, 352 |
| BfaI | CTAG | 1 cut(s) | 104 |
| BisI | GCNGC | 4 cut(s) | 168, 171, 201, 204 |
| BlnI | CCTAGG | 1 cut(s) | 103 |
| BlsI | GCNGC | 4 cut(s) | 169, 172, 202, 205 |
| Bme18I | GGWCC | 1 cut(s) | 107 |
| BmgT120I | GGNCC | 2 cut(s) | 107, 172 |
| BmiI | GGNNCC | 1 cut(s) | 166 |
| BpiI | GAAGAC | 1 cut(s) | 141 |
| BsaHI | GRCGYC | 1 cut(s) | 45 |
| BsaJI | CCNNGG | 2 cut(s) | 103, 168 |
| BsaWI | WCCGGW | 1 cut(s) | 401 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 401 |
| Bse118I | RCCGGY | 1 cut(s) | 376 |
| BseDI | CCNNGG | 2 cut(s) | 103, 168 |
| BseGI | GGATG | 4 cut(s) | 97, 277, 319, 352 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 401 |
| BseMII | CTCAG | 1 cut(s) | 137 |
| BseRI | GAGGAG | 4 cut(s) | 83, 143, 379, 397 |
| BseSI | GKGCMC | 1 cut(s) | 373 |
| Bsh1236I | CGCG | 2 cut(s) | 112, 170 |
| BshFI | GGCC | 3 cut(s) | 102, 173, 284 |
| BsiSI | CCGG | 3 cut(s) | 338, 377, 402 |
| BslFI | GGGAC | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 401 |
| BsmAI | GTCTC | 2 cut(s) | 113, 352 |
| BsmBI | CGTCTC | 1 cut(s) | 352 |
| BsmFI | GGGAC | 1 cut(s) | 92 |
| BsnI | GGCC | 3 cut(s) | 102, 173, 284 |
| Bsp1286I | GDGCHC | 1 cut(s) | 373 |
| Bsp143I | GATC | 3 cut(s) | 12, 448, 508 |
| BspACI | CCGC | 6 cut(s) | 110, 168, 170, 200, 203, 206 |
| BspANI | GGCC | 3 cut(s) | 102, 173, 284 |
| BspCNI | CTCAG | 1 cut(s) | 136 |
| BspFNI | CGCG | 2 cut(s) | 112, 170 |
| BspLI | GGNNCC | 1 cut(s) | 166 |
| BsrFI | RCCGGY | 1 cut(s) | 376 |
| BssAI | RCCGGY | 1 cut(s) | 376 |
| BssECI | CCNNGG | 2 cut(s) | 103, 168 |
| BssMI | GATC | 3 cut(s) | 12, 448, 508 |
| BssNI | GRCGYC | 1 cut(s) | 45 |
| BssT1I | CCWWGG | 1 cut(s) | 103 |
| Bst4CI | ACNGT | 2 cut(s) | 25, 140 |
| BstACI | GRCGYC | 1 cut(s) | 45 |
| BstC8I | GCNNGC | 1 cut(s) | 378 |
| BstDEI | CTNAG | 1 cut(s) | 123 |
| BstDSI | CCRYGG | 1 cut(s) | 168 |
| BstF5I | GGATG | 4 cut(s) | 97, 277, 319, 352 |
| BstFNI | CGCG | 2 cut(s) | 112, 170 |
| BstHHI | GCGC | 1 cut(s) | 337 |
| BstKTI | GATC | 3 cut(s) | 15, 451, 511 |
| BstMAI | GTCTC | 2 cut(s) | 113, 352 |
| BstMBI | GATC | 3 cut(s) | 12, 448, 508 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 53, 332, 377 |
| BstSLI | GKGCMC | 1 cut(s) | 373 |
| BstUI | CGCG | 2 cut(s) | 112, 170 |
| BstV2I | GAAGAC | 1 cut(s) | 141 |
| BsuRI | GGCC | 3 cut(s) | 102, 173, 284 |
| BtgI | CCRYGG | 1 cut(s) | 168 |
| BtgZI | GCGATG | 1 cut(s) | 37 |
| BtsCI | GGATG | 4 cut(s) | 97, 277, 319, 352 |
| Cac8I | GCNNGC | 1 cut(s) | 378 |
| CfoI | GCGC | 1 cut(s) | 337 |
| Cfr10I | RCCGGY | 1 cut(s) | 376 |
| Cfr13I | GGNCC | 2 cut(s) | 107, 172 |
| Cfr42I | CCGCGG | 1 cut(s) | 171 |
| CseI | GACGC | 1 cut(s) | 53 |
| Csp6I | GTAC | 2 cut(s) | 40, 488 |
| CviJI | RGCY | 7 cut(s) | 102, 167, 173, 181, 229, 284, 376 |
| CviKI_1 | RGCY | 7 cut(s) | 102, 167, 173, 181, 229, 284, 376 |
| CviQI | GTAC | 2 cut(s) | 40, 488 |
| DdeI | CTNAG | 1 cut(s) | 123 |
| DpnI | GATC | 3 cut(s) | 14, 450, 510 |
| DpnII | GATC | 3 cut(s) | 12, 448, 508 |
| EciI | GGCGGA | 1 cut(s) | 221 |
| Eco130I | CCWWGG | 1 cut(s) | 103 |
| Eco47I | GGWCC | 1 cut(s) | 107 |
| EcoT14I | CCWWGG | 1 cut(s) | 103 |
| ErhI | CCWWGG | 1 cut(s) | 103 |
| Esp3I | CGTCTC | 1 cut(s) | 352 |
| FaiI | YATR | 1 cut(s) | 117 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 429, 461 |
| FaqI | GGGAC | 1 cut(s) | 92 |
| FbaI | TGATCA | 2 cut(s) | 448, 508 |
| FblI | GTMKAC | 1 cut(s) | 27 |
| Fnu4HI | GCNGC | 4 cut(s) | 168, 171, 201, 204 |
| FokI | GGATG | 4 cut(s) | 104, 284, 326, 359 |
| Fsp4HI | GCNGC | 4 cut(s) | 168, 171, 201, 204 |
| FspBI | CTAG | 1 cut(s) | 104 |
| GlaI | GCGC | 1 cut(s) | 336 |
| GluI | GCNGC | 4 cut(s) | 168, 171, 201, 204 |
| HaeIII | GGCC | 3 cut(s) | 102, 173, 284 |
| HapII | CCGG | 3 cut(s) | 338, 377, 402 |
| HgaI | GACGC | 1 cut(s) | 53 |
| HhaI | GCGC | 1 cut(s) | 337 |
| Hin1I | GRCGYC | 1 cut(s) | 45 |
| Hin6I | GCGC | 1 cut(s) | 335 |
| HinP1I | GCGC | 1 cut(s) | 335 |
| HincII | GTYRAC | 1 cut(s) | 28 |
| HindII | GTYRAC | 1 cut(s) | 28 |
| HinfI | GANTC | 2 cut(s) | 7, 121 |
| HpaII | CCGG | 3 cut(s) | 338, 377, 402 |
| HphI | GGTGA | 1 cut(s) | 287 |
| Hpy166II | GTNNAC | 1 cut(s) | 28 |
| Hpy188I | TCNGA | 3 cut(s) | 12, 396, 469 |
| Hpy188III | TCNNGA | 2 cut(s) | 125, 517 |
| Hpy8I | GTNNAC | 1 cut(s) | 28 |
| Hpy99I | CGWCG | 6 cut(s) | 32, 47, 101, 193, 238, 253 |
| HpyAV | CCTTC | 2 cut(s) | 143, 155 |
| HpyCH4III | ACNGT | 2 cut(s) | 25, 140 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 53, 332, 377 |
| HpyF3I | CTNAG | 1 cut(s) | 123 |
| Hsp92I | GRCGYC | 1 cut(s) | 45 |
| HspAI | GCGC | 1 cut(s) | 335 |
| KroI | GCCGGC | 1 cut(s) | 376 |
| KroNI | GCCGGC | 1 cut(s) | 378 |
| Ksp22I | TGATCA | 2 cut(s) | 448, 508 |
| KspI | CCGCGG | 1 cut(s) | 171 |
| Kzo9I | GATC | 3 cut(s) | 12, 448, 508 |
| LmnI | GCTCC | 1 cut(s) | 164 |
| LpnPI | CCDG | 5 cut(s) | 110, 351, 390, 415, 444 |
| MaeI | CTAG | 1 cut(s) | 104 |
| MaeIII | GTNAC | 1 cut(s) | 275 |
| MalI | GATC | 3 cut(s) | 14, 450, 510 |
| MboI | GATC | 3 cut(s) | 12, 448, 508 |
| MboII | GAAGA | 3 cut(s) | 146, 158, 485 |
| MhlI | GDGCHC | 1 cut(s) | 373 |
| MluCI | AATT | 3 cut(s) | 184, 512, 523 |
| MlyI | GAGTC | 1 cut(s) | 115 |
| MroNI | GCCGGC | 1 cut(s) | 376 |
| MseI | TTAA | 2 cut(s) | 258, 408 |
| MspA1I | CMGCKG | 2 cut(s) | 170, 200 |
| MspI | CCGG | 3 cut(s) | 338, 377, 402 |
| MvnI | CGCG | 2 cut(s) | 112, 170 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 53, 332, 377 |
| NaeI | GCCGGC | 1 cut(s) | 378 |
| NdeII | GATC | 3 cut(s) | 12, 448, 508 |
| NgoMIV | GCCGGC | 1 cut(s) | 376 |
| NlaIV | GGNNCC | 1 cut(s) | 166 |
| NmeAIII | GCCGAG | 1 cut(s) | 352 |
| NmuCI | GTSAC | 1 cut(s) | 275 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 24, 33, 93 |
| PdiI | GCCGGC | 1 cut(s) | 378 |
| PfeI | GAWTC | 1 cut(s) | 7 |
| PflFI | GACNNNGTC | 1 cut(s) | 23 |
| PkrI | GCNGC | 4 cut(s) | 169, 172, 202, 205 |
| PleI | GAGTC | 1 cut(s) | 115 |
| PpsI | GAGTC | 1 cut(s) | 115 |
| PspN4I | GGNNCC | 1 cut(s) | 166 |
| PspPI | GGNCC | 2 cut(s) | 107, 172 |
| PsyI | GACNNNGTC | 1 cut(s) | 23 |
| RsaI | GTAC | 2 cut(s) | 41, 489 |
| RsaNI | GTAC | 2 cut(s) | 40, 488 |
| SacII | CCGCGG | 1 cut(s) | 171 |
| SalI | GTCGAC | 1 cut(s) | 26 |
| SaqAI | TTAA | 2 cut(s) | 258, 408 |
| SatI | GCNGC | 4 cut(s) | 168, 171, 201, 204 |
| Sau3AI | GATC | 3 cut(s) | 12, 448, 508 |
| Sau96I | GGNCC | 2 cut(s) | 107, 172 |
| SchI | GAGTC | 1 cut(s) | 115 |
| SduI | GDGCHC | 1 cut(s) | 373 |
| Sfr303I | CCGCGG | 1 cut(s) | 171 |
| SgrBI | CCGCGG | 1 cut(s) | 171 |
| SinI | GGWCC | 1 cut(s) | 107 |
| Sse9I | AATT | 3 cut(s) | 184, 512, 523 |
| SsiI | CCGC | 6 cut(s) | 110, 168, 170, 200, 203, 206 |
| SspMI | CTAG | 1 cut(s) | 104 |
| StyI | CCWWGG | 1 cut(s) | 103 |
| TaaI | ACNGT | 2 cut(s) | 25, 140 |
| TaqI | TCGA | 3 cut(s) | 27, 87, 236 |
| TasI | AATT | 3 cut(s) | 184, 512, 523 |
| TatI | WGTACW | 1 cut(s) | 487 |
| TauI | GCSGC | 4 cut(s) | 170, 173, 203, 206 |
| TfiI | GAWTC | 1 cut(s) | 7 |
| Tru1I | TTAA | 2 cut(s) | 258, 408 |
| Tru9I | TTAA | 2 cut(s) | 258, 408 |
| TseFI | GTSAC | 1 cut(s) | 275 |
| Tsp45I | GTSAC | 1 cut(s) | 275 |
| TspDTI | ATGAA | 3 cut(s) | 450, 471, 486 |
| TspGWI | ACGGA | 1 cut(s) | 376 |
| Tth111I | GACNNNGTC | 1 cut(s) | 23 |
| VpaK11BI | GGWCC | 1 cut(s) | 107 |
| XmaJI | CCTAGG | 1 cut(s) | 103 |
| XmiI | GTMKAC | 1 cut(s) | 27 |
| XspI | CTAG | 1 cut(s) | 104 |