DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 171 |
| Acc65I | GGTACC | 1 cut(s) | 118 |
| AccB1I | GGYRCC | 1 cut(s) | 118 |
| AccII | CGCG | 1 cut(s) | 124 |
| AciI | CCGC | 5 cut(s) | 34, 38, 122, 152, 415 |
| AcoI | YGGCCR | 1 cut(s) | 35 |
| AcyI | GRCGYC | 1 cut(s) | 168 |
| AfaI | GTAC | 1 cut(s) | 120 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 61, 157, 208, 393 |
| AjiI | CACGTC | 1 cut(s) | 238 |
| AluBI | AGCT | 1 cut(s) | 21 |
| AluI | AGCT | 1 cut(s) | 21 |
| Alw21I | GWGCWC | 1 cut(s) | 23 |
| Alw26I | GTCTC | 1 cut(s) | 344 |
| AoxI | GGCC | 4 cut(s) | 35, 110, 198, 274 |
| ApeKI | GCWGC | 1 cut(s) | 40 |
| Asp718I | GGTACC | 1 cut(s) | 118 |
| AspA2I | CCTAGG | 1 cut(s) | 113 |
| AspLEI | GCGC | 1 cut(s) | 13 |
| AspS9I | GGNCC | 2 cut(s) | 148, 199 |
| AsuHPI | GGTGA | 1 cut(s) | 279 |
| AvaII | GGWCC | 1 cut(s) | 148 |
| AvrII | CCTAGG | 1 cut(s) | 113 |
| BaeGI | GKGCMC | 1 cut(s) | 365 |
| BanI | GGYRCC | 1 cut(s) | 118 |
| BanII | GRGCYC | 1 cut(s) | 23 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 75, 107 |
| Bbv12I | GWGCWC | 1 cut(s) | 23 |
| BbvI | GCAGC | 1 cut(s) | 27 |
| BccI | CCATC | 4 cut(s) | 71, 180, 258, 421 |
| BceAI | ACGGC | 1 cut(s) | 125 |
| BcoDI | GTCTC | 1 cut(s) | 344 |
| BfaI | CTAG | 1 cut(s) | 114 |
| BfoI | RGCGCY | 1 cut(s) | 14 |
| BisI | GCNGC | 3 cut(s) | 35, 38, 41 |
| BlnI | CCTAGG | 1 cut(s) | 113 |
| BlsI | GCNGC | 3 cut(s) | 36, 39, 42 |
| Bme18I | GGWCC | 1 cut(s) | 148 |
| BmgBI | CACGTC | 1 cut(s) | 238 |
| BmgT120I | GGNCC | 2 cut(s) | 148, 199 |
| BmiI | GGNNCC | 3 cut(s) | 66, 120, 150 |
| BpmI | CTGGAG | 1 cut(s) | 9 |
| BsaHI | GRCGYC | 1 cut(s) | 168 |
| BsaJI | CCNNGG | 3 cut(s) | 4, 113, 155 |
| BsaWI | WCCGGW | 1 cut(s) | 393 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 61, 157, 208, 393 |
| Bse118I | RCCGGY | 2 cut(s) | 242, 368 |
| Bse1I | ACTGG | 1 cut(s) | 150 |
| BseDI | CCNNGG | 3 cut(s) | 4, 113, 155 |
| BseGI | GGATG | 3 cut(s) | 182, 269, 311 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 61, 157, 208, 393 |
| BseNI | ACTGG | 1 cut(s) | 150 |
| BseRI | GAGGAG | 2 cut(s) | 371, 389 |
| BseSI | GKGCMC | 1 cut(s) | 365 |
| BseX3I | CGGCCG | 1 cut(s) | 35 |
| BseXI | GCAGC | 1 cut(s) | 27 |
| BseYI | CCCAGC | 1 cut(s) | 201 |
| Bsh1236I | CGCG | 1 cut(s) | 124 |
| Bsh1285I | CGRYCG | 2 cut(s) | 38, 62 |
| BshFI | GGCC | 4 cut(s) | 37, 112, 200, 276 |
| BshNI | GGYRCC | 1 cut(s) | 118 |
| BsiEI | CGRYCG | 2 cut(s) | 38, 62 |
| BsiHKAI | GWGCWC | 1 cut(s) | 23 |
| BsiSI | CCGG | 3 cut(s) | 243, 369, 394 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 61, 157, 208, 393 |
| BsmAI | GTCTC | 1 cut(s) | 344 |
| BsmBI | CGTCTC | 1 cut(s) | 344 |
| BsnI | GGCC | 4 cut(s) | 37, 112, 200, 276 |
| Bsp1286I | GDGCHC | 2 cut(s) | 23, 365 |
| Bsp143I | GATC | 3 cut(s) | 59, 70, 88 |
| BspACI | CCGC | 5 cut(s) | 34, 38, 122, 152, 415 |
| BspANI | GGCC | 4 cut(s) | 37, 112, 200, 276 |
| BspFNI | CGCG | 1 cut(s) | 124 |
| BspLI | GGNNCC | 3 cut(s) | 66, 120, 150 |
| BspT107I | GGYRCC | 1 cut(s) | 118 |
| BsrFI | RCCGGY | 2 cut(s) | 242, 368 |
| BsrI | ACTGG | 1 cut(s) | 150 |
| BssAI | RCCGGY | 2 cut(s) | 242, 368 |
| BssECI | CCNNGG | 3 cut(s) | 4, 113, 155 |
| BssMI | GATC | 3 cut(s) | 59, 70, 88 |
| BssNI | GRCGYC | 1 cut(s) | 168 |
| BssT1I | CCWWGG | 2 cut(s) | 4, 113 |
| Bst6I | CTCTTC | 1 cut(s) | 133 |
| BstACI | GRCGYC | 1 cut(s) | 168 |
| BstC8I | GCNNGC | 1 cut(s) | 370 |
| BstDSI | CCRYGG | 1 cut(s) | 155 |
| BstF5I | GGATG | 3 cut(s) | 182, 269, 311 |
| BstFNI | CGCG | 1 cut(s) | 124 |
| BstH2I | RGCGCY | 1 cut(s) | 14 |
| BstHHI | GCGC | 1 cut(s) | 13 |
| BstKTI | GATC | 3 cut(s) | 62, 73, 91 |
| BstMAI | GTCTC | 1 cut(s) | 344 |
| BstMBI | GATC | 3 cut(s) | 59, 70, 88 |
| BstMCI | CGRYCG | 2 cut(s) | 38, 62 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 40, 130, 369 |
| BstNSI | RCATGY | 1 cut(s) | 424 |
| BstSLI | GKGCMC | 1 cut(s) | 365 |
| BstUI | CGCG | 1 cut(s) | 124 |
| BstV1I | GCAGC | 1 cut(s) | 27 |
| BstZI | CGGCCG | 1 cut(s) | 35 |
| BsuRI | GGCC | 4 cut(s) | 37, 112, 200, 276 |
| BtgI | CCRYGG | 1 cut(s) | 155 |
| BtrI | CACGTC | 1 cut(s) | 238 |
| BtsCI | GGATG | 3 cut(s) | 182, 269, 311 |
| Cac8I | GCNNGC | 1 cut(s) | 370 |
| CciNI | GCGGCCGC | 1 cut(s) | 35 |
| CfoI | GCGC | 1 cut(s) | 13 |
| Cfr10I | RCCGGY | 2 cut(s) | 242, 368 |
| Cfr13I | GGNCC | 2 cut(s) | 148, 199 |
| Csp6I | GTAC | 1 cut(s) | 119 |
| CviAII | CATG | 1 cut(s) | 421 |
| CviJI | RGCY | 9 cut(s) | 21, 37, 86, 112, 161, 200, 221, 276, 368 |
| CviKI_1 | RGCY | 9 cut(s) | 21, 37, 86, 112, 161, 200, 221, 276, 368 |
| CviQI | GTAC | 1 cut(s) | 119 |
| DpnI | GATC | 3 cut(s) | 61, 72, 90 |
| DpnII | GATC | 3 cut(s) | 59, 70, 88 |
| EaeI | YGGCCR | 1 cut(s) | 35 |
| EagI | CGGCCG | 1 cut(s) | 35 |
| Eam1104I | CTCTTC | 1 cut(s) | 133 |
| EarI | CTCTTC | 1 cut(s) | 133 |
| Ecl136II | GAGCTC | 1 cut(s) | 21 |
| EclXI | CGGCCG | 1 cut(s) | 35 |
| Eco130I | CCWWGG | 2 cut(s) | 4, 113 |
| Eco24I | GRGCYC | 1 cut(s) | 23 |
| Eco47I | GGWCC | 1 cut(s) | 148 |
| Eco52I | CGGCCG | 1 cut(s) | 35 |
| Eco53kI | GAGCTC | 1 cut(s) | 21 |
| EcoICRI | GAGCTC | 1 cut(s) | 21 |
| EcoT14I | CCWWGG | 2 cut(s) | 4, 113 |
| EcoT38I | GRGCYC | 1 cut(s) | 23 |
| ErhI | CCWWGG | 2 cut(s) | 4, 113 |
| Esp3I | CGTCTC | 1 cut(s) | 344 |
| FaeI | CATG | 1 cut(s) | 424 |
| FaiI | YATR | 2 cut(s) | 195, 422 |
| FatI | CATG | 1 cut(s) | 420 |
| FauI | CCCGC | 1 cut(s) | 159 |
| Fnu4HI | GCNGC | 3 cut(s) | 35, 38, 41 |
| FokI | GGATG | 3 cut(s) | 189, 276, 318 |
| FriOI | GRGCYC | 1 cut(s) | 23 |
| Fsp4HI | GCNGC | 3 cut(s) | 35, 38, 41 |
| FspBI | CTAG | 1 cut(s) | 114 |
| GlaI | GCGC | 1 cut(s) | 12 |
| GluI | GCNGC | 3 cut(s) | 35, 38, 41 |
| GsaI | CCCAGC | 1 cut(s) | 205 |
| GsuI | CTGGAG | 1 cut(s) | 9 |
| HaeII | RGCGCY | 1 cut(s) | 14 |
| HaeIII | GGCC | 4 cut(s) | 37, 112, 200, 276 |
| HapII | CCGG | 3 cut(s) | 243, 369, 394 |
| HhaI | GCGC | 1 cut(s) | 13 |
| Hin1I | GRCGYC | 1 cut(s) | 168 |
| Hin1II | CATG | 1 cut(s) | 424 |
| Hin6I | GCGC | 1 cut(s) | 11 |
| HinP1I | GCGC | 1 cut(s) | 11 |
| HinfI | GANTC | 1 cut(s) | 180 |
| HpaII | CCGG | 3 cut(s) | 243, 369, 394 |
| HphI | GGTGA | 1 cut(s) | 279 |
| Hpy166II | GTNNAC | 1 cut(s) | 235 |
| Hpy188I | TCNGA | 6 cut(s) | 18, 47, 166, 176, 185, 388 |
| Hpy188III | TCNNGA | 2 cut(s) | 68, 74 |
| Hpy8I | GTNNAC | 1 cut(s) | 235 |
| Hpy99I | CGWCG | 7 cut(s) | 60, 111, 129, 173, 176, 230, 242 |
| HpyCH4IV | ACGT | 2 cut(s) | 168, 237 |
| HpyCH4V | TGCA | 1 cut(s) | 420 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 40, 130, 369 |
| HpySE526I | ACGT | 2 cut(s) | 168, 237 |
| Hsp92I | GRCGYC | 1 cut(s) | 168 |
| Hsp92II | CATG | 1 cut(s) | 424 |
| HspAI | GCGC | 1 cut(s) | 11 |
| KpnI | GGTACC | 1 cut(s) | 122 |
| KroI | GCCGGC | 1 cut(s) | 368 |
| KroNI | GCCGGC | 1 cut(s) | 370 |
| Kzo9I | GATC | 3 cut(s) | 59, 70, 88 |
| LmnI | GCTCC | 1 cut(s) | 8 |
| LpnPI | CCDG | 8 cut(s) | 39, 81, 131, 191, 215, 256, 382, 407 |
| Lsp1109I | GCAGC | 1 cut(s) | 27 |
| MaeI | CTAG | 1 cut(s) | 114 |
| MaeII | ACGT | 2 cut(s) | 168, 237 |
| MaeIII | GTNAC | 1 cut(s) | 267 |
| MalI | GATC | 3 cut(s) | 61, 72, 90 |
| MboI | GATC | 3 cut(s) | 59, 70, 88 |
| MboII | GAAGA | 1 cut(s) | 150 |
| MhlI | GDGCHC | 2 cut(s) | 23, 365 |
| MmeI | TCCRAC | 1 cut(s) | 154 |
| MroNI | GCCGGC | 1 cut(s) | 368 |
| MseI | TTAA | 2 cut(s) | 250, 400 |
| MslI | CAYNNNNRTG | 1 cut(s) | 425 |
| MspA1I | CMGCKG | 1 cut(s) | 40 |
| MspI | CCGG | 3 cut(s) | 243, 369, 394 |
| MvnI | CGCG | 1 cut(s) | 124 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 40, 130, 369 |
| NaeI | GCCGGC | 1 cut(s) | 370 |
| NdeII | GATC | 3 cut(s) | 59, 70, 88 |
| NgoMIV | GCCGGC | 1 cut(s) | 368 |
| NlaIII | CATG | 1 cut(s) | 424 |
| NlaIV | GGNNCC | 3 cut(s) | 66, 120, 150 |
| NmeAIII | GCCGAG | 1 cut(s) | 344 |
| NmuCI | GTSAC | 1 cut(s) | 267 |
| NotI | GCGGCCGC | 1 cut(s) | 35 |
| NspI | RCATGY | 1 cut(s) | 424 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 222, 234 |
| PdiI | GCCGGC | 1 cut(s) | 370 |
| PfeI | GAWTC | 1 cut(s) | 180 |
| PflFI | GACNNNGTC | 1 cut(s) | 170 |
| PkrI | GCNGC | 3 cut(s) | 36, 39, 42 |
| Ple19I | CGATCG | 1 cut(s) | 62 |
| Psp124BI | GAGCTC | 1 cut(s) | 23 |
| PspFI | CCCAGC | 1 cut(s) | 201 |
| PspN4I | GGNNCC | 3 cut(s) | 66, 120, 150 |
| PspPI | GGNCC | 2 cut(s) | 148, 199 |
| PsyI | GACNNNGTC | 1 cut(s) | 170 |
| PvuI | CGATCG | 1 cut(s) | 62 |
| RsaI | GTAC | 1 cut(s) | 120 |
| RsaNI | GTAC | 1 cut(s) | 119 |
| RseI | CAYNNNNRTG | 1 cut(s) | 425 |
| SacI | GAGCTC | 1 cut(s) | 23 |
| SaqAI | TTAA | 2 cut(s) | 250, 400 |
| SatI | GCNGC | 3 cut(s) | 35, 38, 41 |
| Sau3AI | GATC | 3 cut(s) | 59, 70, 88 |
| Sau96I | GGNCC | 2 cut(s) | 148, 199 |
| SduI | GDGCHC | 2 cut(s) | 23, 365 |
| SetI | ASST | 4 cut(s) | 23, 171, 240, 340 |
| SgrAI | CRCCGGYG | 1 cut(s) | 242 |
| SinI | GGWCC | 1 cut(s) | 148 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 425 |
| SsiI | CCGC | 5 cut(s) | 34, 38, 122, 152, 415 |
| SspMI | CTAG | 1 cut(s) | 114 |
| SstI | GAGCTC | 1 cut(s) | 23 |
| StyI | CCWWGG | 2 cut(s) | 4, 113 |
| TaiI | ACGT | 2 cut(s) | 171, 240 |
| TaqI | TCGA | 3 cut(s) | 228, 330, 425 |
| TauI | GCSGC | 2 cut(s) | 37, 40 |
| TfiI | GAWTC | 1 cut(s) | 180 |
| Tru1I | TTAA | 2 cut(s) | 250, 400 |
| Tru9I | TTAA | 2 cut(s) | 250, 400 |
| TseFI | GTSAC | 1 cut(s) | 267 |
| TseI | GCWGC | 1 cut(s) | 40 |
| Tsp45I | GTSAC | 1 cut(s) | 267 |
| TspDTI | ATGAA | 1 cut(s) | 193 |
| TspGWI | ACGGA | 2 cut(s) | 114, 368 |
| Tth111I | GACNNNGTC | 1 cut(s) | 170 |
| VpaK11BI | GGWCC | 1 cut(s) | 148 |
| XceI | RCATGY | 1 cut(s) | 424 |
| XmaJI | CCTAGG | 1 cut(s) | 113 |
| XspI | CTAG | 1 cut(s) | 114 |
| ZraI | GACGTC | 1 cut(s) | 169 |