Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Forward (+)

62179689 .. 62189907

Gene Structure 10219 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00126810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4575 bp

ATGGCGGACGACGAGCAACCGATCGTGACCGGGAGGCGCCGGAGCCGAGGAGCCGAGGCGTCGGCGAGGTCCAGCGCCCTGGAGCGCCTCCGAGCTCTCCGGCAGGGAGGCCGCCGCTCGGAAAACGCCGACGACCGGTTCCAGATCAAGATGGAGAAGCCGATCTACGACACGGTCGACGAGGACGAGTACGACGCCATCATCGCCAAGCGGCGCGAGGAGTTCGGGGACTTCATCGAGGACGACGACGGCCTAGGGTACCGCGACGACGGCGAGGAAGAGGACTGGGACCCGCCGCGGGCTTCCGACGTCCCCAATTCGTCGCTTACGGCGGCGGCTGCGATGATGGGGAAGCAGAGGCTTTCGTCGATGTTCACGTCGTCGGTGTTTAATAAAAGTAGGGATGGTGACAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCCGAGTTTGCGCCGGACGAGGCTGATAGAGAGAAACGGAGGAGGGCACAGCCGGCAAGGAGTTTTGTTCCGATTGCCGGTGTTAAGAGTGAGAGAGTGAGTAATGGGGGTGTTGAGTTATCAAGGAGGCTTGAATTGGATAGAATTGTTGCAAATGGTAGTGGTTCTGTGCATAGTAAGGAAATGGATAAAGATTCTGAGGGCAAGAAGGAGTTGGATTTCGGAAATTGTCAGCCCCAAATTGAGCAATTCCATGATTTTTTGGAGAATAGTGAGGTGCCCGAGGAGAAGAAGACTGAGGATTCTGCTGAGGTGCAGGCGGAGCCTGTGGTGAAGAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATGAGGATCCGGCATTGAGTGCTATGGCAGGATGGAAGGCCGCGATGACTGGTGGGGCCGGAGTTACTGGTGGCAGCGGCAATGTGGAGGTCAAAAGTGACTCAATTAGTGAAGGGAGTTCAGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAATATGGGCACTGTTTATCTATTTGGGAAGGTTAAAGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTAAAGAACATGCAAAGATGTGTCTATGCAATTCCGGACAGTCCTTTATTTCAAACTGACGAAATGATGAAGCTCGAAAAGGATGCCGAGGACTCAAGGATTTCACCCACAGATTTTCGTAAGAAGCTGCATGATGCGGCTTCAGAATTAAAGAATGAATTAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGGCACCTGTCAAGAGGAACTATGCTTTTGAGCGATCTGACATACCTGCGGGGGAGAATTATGTACTGAAGATCAATTATCCATTCCGGGATCCACCACTATCAGCAGATCTAAAAGGAGAAACTTTTTGTGCTCTTCTAGGAACTCATAGCAGCGCATTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGGACCTTCTTGGCTATCAGTTTCAAATTTTTCCATCTGCCCAGCTCCACAACGGATTATGTCATTTTTACTGTACAGGTAAGCTGGTGCAAATTTGAGGTGATCGTGAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACAAAGAAAACTGTAGATATTCCTCAAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTATCAATGAGAAGCAGAATGCTAACGAAATTGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCCGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGTGGCATATTTCCTATGGGATTTACTAAAGTGGCTGCAGATAAGAATTCTAAAGCCGGATCAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTAAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTTGTTGGACATAATATATCTGGATTTGATCTGGATGTTCTTCTCCACAGAGCTCAGGTTTGCAGAGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATCATGTCCTGCATTGCTGGTCGGCTGTTGTGTGATACGTACTTGTGTTCCCGTGACTTATTAAAGGAGGTCAGCTATTCCTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCGCATGATATTCCTAGAATGTTCCAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCATGGTTGTCTATGGGACTCATGTTTCATTTGAGTGCTCTTCCCCTCACACGTCAGCTGACTAATATAAGTGGTAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTAGAATATCTCTTACTGCATGCTTTTCATGCAAAAAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGTGTTGAGGAAAAAGCTGCTGATGAGTTGGATGTAAATGATGTAAATGTTGATAATAATGCACAGAGTGGTCATGGAAAAGGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTATTGGAGCCAAAAAAAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAACATATGCTTCACAACTGTTGAAAGAAATTCAGAAGGATTGGTTTCTCATTTACCTTCTAGTCAAAGACCTGGACTTTTACCTGAGTTGTTAAAAAATTTAGTTGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAACTTGACATTCAACAGCAAGCTCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAACTCAAGATTTTATGCAAAGCCACTGGCAGAGCTTATAACACTACGGGGCAGGGAGATCCTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTAATCTATGGTGATACGGACTCAATAATGATTTATAGTGGACTGGATGATAGTGCGAAAGCAAAAGCAATTGCTGTGAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGACGGCTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCGGCTGTGAAGGAGCTAACGAAAAACGAGAAAAAATACGAGGTTATCGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGTTTACTTTCAAAGGAACTGGGAGATTTCTGCTTAAGTCAAATTTTGTCAGGAGGGTCATCTGAAGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTGCCCTCGAGAAATATATCATCACCAAGACATTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAGTGGTTACTCTACTGGTTGCTCTGTTGGTGATACAGTCCCTTATATTATTTGCTGTGAGCAGGTTTGTGATGCAGTTGGCACAAATTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAGATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCTGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACCAGCCCCCAACGCTTGGCTGATTGTTTAGGGATTGACTCATCCAAGTTCAAATCGAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTGTTGACGATGAAGAGAGATATCGGGATTGCGAGTCTTTGATCTTAGCATGTCCTAGCTGCTCTGGTACTTTTGATTGTCCAGCCATCTTCAACTCCATTGCCAAGTCAATCAATGAAAAGCCAACAAGGCCACAAGGAGAAGAATCTTCCAGCGATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCCAATCAGGTCAAAAGGCAAGCAGAGGGTTTTGTTTCAATGTACTACAGGGGCATAATGACGTGTGATGATGAAACTTGCAAATACACCACACGAAGTCTCAACCTCCGGCTTGTTGGTGATGCTGAGAAAGGAACAGTCTGTCCAAACTATCCTCACTGCAATGGGCGTCTTGTAAGAAAGTACACGGAAGCTGATTTGTACAAACAGCTCTCATTTTTCTGCCATGTCTTGGATACAGTACGCTGCATAGAAAAGATGGAACCTAAGACTAGACTGCCGGTAGAGTTAGAGTTGACCAAAATTCGCCCAATTGTGACTTTGGCTGCCTCAACAGTTGAAAGGATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTGCAAGATCTTTTTGTTACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1524

Amino Acids

170.16

Weight (kDa)

6.41

Isoelectric Point (pI)

44.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	27 - 97	1.2e-19	DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1	PF03104	483 - 744	2.7e-21	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	810 - 1277	2.5e-125	DNA polymerase family B
zf-DNA_Pol	PF08996	1315 - 1521	1.7e-43	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3041
AatII	GACGTC	1 cut(s)	312
Acc36I	ACCTGC	2 cut(s)	1351, 3655
Acc65I	GGTACC	2 cut(s)	258, 3836
AccB1I	GGYRCC	5 cut(s)	36, 258, 733, 1300, 3836
AccB7I	CCANNNNNTGG	2 cut(s)	3853, 4402
AccBSI	CCGCTC	1 cut(s)	117
AccI	GTMKAC	1 cut(s)	177
AccII	CGCG	4 cut(s)	216, 264, 298, 875
AccIII	TCCGGA	1 cut(s)	1129
AclWI	GGATC	9 cut(s)	833, 846, 1281, 1382, 1395, 1900, 2153, 3055, 3087
AcsI	RAATTY	9 cut(s)	1514, 1580, 1879, 2800, 2869, 3408, 3688, 3895, 4473
AcuI	CTGAAG	5 cut(s)	1221, 1385, 2555, 2981, 3450
AcyI	GRCGYC	5 cut(s)	37, 59, 195, 309, 4339
AdeI	CACNNNGTG	1 cut(s)	2639
AflII	CTTAAG	1 cut(s)	3400
AflIII	ACRYGT	3 cut(s)	2399, 3574, 4232
AgeI	ACCGGT	1 cut(s)	135
AjiI	CACGTC	3 cut(s)	378, 2402, 4233
AjnI	CCWGG	4 cut(s)	78, 1792, 2140, 2842
AleI	CACNNNNGTG	1 cut(s)	1766
AloI	GAACNNNNNNTCC	2 cut(s)	4297, 4329
Alw21I	GWGCWC	4 cut(s)	97, 1432, 2032, 2389
Alw26I	GTCTC	6 cut(s)	1624, 1726, 2336, 2927, 4161, 4274
AlwI	GGATC	9 cut(s)	833, 846, 1281, 1382, 1395, 1900, 2153, 3055, 3087
Ama87I	CYCGRG	2 cut(s)	737, 3503
Aor13HI	TCCGGA	1 cut(s)	1129
ApoI	RAATTY	9 cut(s)	1514, 1580, 1879, 2800, 2869, 3408, 3688, 3895, 4473
AsiGI	ACCGGT	1 cut(s)	135
Asp700I	GAANNNNTTC	3 cut(s)	3447, 3919, 4515
Asp718I	GGTACC	2 cut(s)	258, 3836
AspA2I	CCTAGG	1 cut(s)	253
AspLEI	GCGC	8 cut(s)	39, 77, 87, 216, 469, 1454, 3589, 4116
AsuC2I	CCSGG	3 cut(s)	31, 1385, 3706
AsuHPI	GGTGA	9 cut(s)	419, 799, 1191, 1600, 3131, 3511, 3644, 4151, 4301
AvaI	CYCGRG	2 cut(s)	737, 3503
AvaII	GGWCC	7 cut(s)	69, 289, 1022, 1491, 2061, 2663, 4522
AvrII	CCTAGG	1 cut(s)	253
BaeGI	GKGCMC	4 cut(s)	505, 738, 1037, 2461
BamHI	GGATCC	2 cut(s)	838, 1387
BanI	GGYRCC	5 cut(s)	36, 258, 733, 1300, 3836
BanII	GRGCYC	2 cut(s)	97, 2032
BarI	GAAGNNNNNNTAC	2 cut(s)	149, 181
BbsI	GAAGAC	1 cut(s)	755
Bbv12I	GWGCWC	4 cut(s)	97, 1432, 2032, 2389
BbvCI	CCTCAGC	1 cut(s)	765
BceAI	ACGGC	4 cut(s)	265, 286, 345, 3256
BcgI	CGANNNNNNTGC	4 cut(s)	1160, 1194, 2330, 2364
BciT130I	CCWGG	4 cut(s)	80, 1794, 2142, 2844
BciVI	GTATCC	3 cut(s)	2533, 4399, 4536
BcnI	CCSGG	3 cut(s)	31, 1385, 3706
BcoDI	GTCTC	6 cut(s)	1624, 1726, 2336, 2927, 4161, 4274
BfaI	CTAG	7 cut(s)	254, 1436, 2292, 2832, 3723, 3996, 4443
BfmI	CTRYAG	3 cut(s)	1641, 1869, 4216
BfoI	RGCGCY	3 cut(s)	40, 78, 88
BfrI	CTTAAG	1 cut(s)	3400
BfuAI	ACCTGC	2 cut(s)	1351, 3655
BfuI	GTATCC	3 cut(s)	2533, 4399, 4536
BglI	GCCNNNNNGGC	1 cut(s)	4069
BglII	AGATCT	2 cut(s)	1405, 4555
BlnI	CCTAGG	1 cut(s)	253
BlpI	GCTNAGC	1 cut(s)	1802
BmcAI	AGTACT	1 cut(s)	3073
Bme1390I	CCNGG	7 cut(s)	31, 80, 1385, 1794, 2142, 2844, 3706
Bme18I	GGWCC	7 cut(s)	69, 289, 1022, 1491, 2061, 2663, 4522
BmeT110I	CYCGRG	2 cut(s)	737, 3503
BmgBI	CACGTC	3 cut(s)	378, 2402, 4233
BmrFI	CCNGG	7 cut(s)	31, 80, 1385, 1794, 2142, 2844, 3706
BmrI	ACTGGG	2 cut(s)	295, 3395
BmuI	ACTGGG	2 cut(s)	295, 3395
BpiI	GAAGAC	1 cut(s)	755
BpmI	CTGGAG	3 cut(s)	101, 2151, 3385
Bpu10I	CCTNAGC	3 cut(s)	765, 2031, 2097
Bpu1102I	GCTNAGC	1 cut(s)	1802
BpuEI	CTTGAG	7 cut(s)	1174, 1638, 1924, 2063, 2909, 2992, 3251
BpuMI	CCSGG	3 cut(s)	31, 1385, 3706
BsaAI	YACGTR	1 cut(s)	2184
BsaBI	GATNNNNATC	2 cut(s)	837, 960
BsaHI	GRCGYC	5 cut(s)	37, 59, 195, 309, 4339
BsaI	GGTCTC	2 cut(s)	1624, 2927
BsaJI	CCNNGG	9 cut(s)	46, 54, 78, 253, 296, 738, 1182, 2141, 2446
BsaWI	WCCGGW	3 cut(s)	135, 1129, 2262
Bse118I	RCCGGY	4 cut(s)	135, 508, 533, 4450
Bse1I	ACTGG	8 cut(s)	290, 886, 904, 3033, 3156, 3368, 3390, 3622
Bse3DI	GCAATG	4 cut(s)	919, 2157, 4038, 4339
Bse8I	GATNNNNATC	2 cut(s)	837, 960
BseAI	TCCGGA	1 cut(s)	1129
BseBI	CCWGG	4 cut(s)	80, 1794, 2142, 2844
BseDI	CCNNGG	9 cut(s)	46, 54, 78, 253, 296, 738, 1182, 2141, 2446
BseJI	GATNNNNATC	2 cut(s)	837, 960
BseMI	GCAATG	4 cut(s)	919, 2157, 4038, 4339
BseNI	ACTGG	8 cut(s)	290, 886, 904, 3033, 3156, 3368, 3390, 3622
BseRI	GAGGAG	5 cut(s)	63, 233, 511, 755, 2899
BseSI	GKGCMC	4 cut(s)	505, 738, 1037, 2461
BseYI	CCCAGC	2 cut(s)	1529, 2251
BsgI	GTGCAG	2 cut(s)	791, 4126
Bsh1236I	CGCG	4 cut(s)	216, 264, 298, 875
Bsh1285I	CGRYCG	3 cut(s)	24, 136, 177
BshNI	GGYRCC	5 cut(s)	36, 258, 733, 1300, 3836
BshTI	ACCGGT	1 cut(s)	135
BsiEI	CGRYCG	3 cut(s)	24, 136, 177
BsiHKAI	GWGCWC	4 cut(s)	97, 1432, 2032, 2389
BsiHKCI	CYCGRG	2 cut(s)	737, 3503
BslFI	GGGAC	8 cut(s)	242, 296, 302, 1504, 2379, 3626, 3772, 4535
BsmAI	GTCTC	6 cut(s)	1624, 1726, 2336, 2927, 4161, 4274
BsmFI	GGGAC	8 cut(s)	242, 296, 302, 1504, 2379, 3626, 3772, 4535
BsmI	GAATGC	5 cut(s)	820, 1603, 1711, 2917, 3261
Bso31I	GGTCTC	2 cut(s)	1624, 2927
BsoBI	CYCGRG	2 cut(s)	737, 3503
Bsp1286I	GDGCHC	8 cut(s)	97, 505, 738, 1037, 1432, 2032, 2389, 2461
Bsp13I	TCCGGA	1 cut(s)	1129
Bsp1407I	TGTACA	3 cut(s)	1561, 3246, 4371
Bsp1720I	GCTNAGC	1 cut(s)	1802
BspEI	TCCGGA	1 cut(s)	1129
BspFNI	CGCG	4 cut(s)	216, 264, 298, 875
BspMAI	CTGCAG	1 cut(s)	1873
BspMI	ACCTGC	2 cut(s)	1351, 3655
BspPI	GGATC	9 cut(s)	833, 846, 1281, 1382, 1395, 1900, 2153, 3055, 3087
BspQI	GCTCTTC	2 cut(s)	1437, 2394
BspT107I	GGYRCC	5 cut(s)	36, 258, 733, 1300, 3836
BspTI	CTTAAG	1 cut(s)	3400
BspTNI	GGTCTC	2 cut(s)	1624, 2927
BsrBI	CCGCTC	1 cut(s)	117
BsrDI	GCAATG	4 cut(s)	919, 2157, 4038, 4339
BsrFI	RCCGGY	4 cut(s)	135, 508, 533, 4450
BsrGI	TGTACA	3 cut(s)	1561, 3246, 4371
BsrI	ACTGG	8 cut(s)	290, 886, 904, 3033, 3156, 3368, 3390, 3622
BssAI	RCCGGY	4 cut(s)	135, 508, 533, 4450
BssECI	CCNNGG	9 cut(s)	46, 54, 78, 253, 296, 738, 1182, 2141, 2446
BssNI	GRCGYC	5 cut(s)	37, 59, 195, 309, 4339
BssT1I	CCWWGG	2 cut(s)	253, 2446
Bst2UI	CCWGG	4 cut(s)	80, 1794, 2142, 2844
Bst6I	CTCTTC	7 cut(s)	273, 792, 1005, 1437, 1781, 2394, 3948
BstACI	GRCGYC	5 cut(s)	37, 59, 195, 309, 4339
BstAFI	CTTAAG	1 cut(s)	3400
BstAPI	GCANNNNNTGC	1 cut(s)	851
BstAUI	TGTACA	3 cut(s)	1561, 3246, 4371
BstBAI	YACGTR	1 cut(s)	2184
BstC8I	GCNNGC	7 cut(s)	300, 510, 774, 2103, 2487, 2955, 4191
BstDSI	CCRYGG	1 cut(s)	296
BstFNI	CGCG	4 cut(s)	216, 264, 298, 875
BstH2I	RGCGCY	3 cut(s)	40, 78, 88
BstHHI	GCGC	8 cut(s)	39, 77, 87, 216, 469, 1454, 3589, 4116
BstMAI	GTCTC	6 cut(s)	1624, 1726, 2336, 2927, 4161, 4274
BstMCI	CGRYCG	3 cut(s)	24, 136, 177
BstNI	CCWGG	4 cut(s)	80, 1794, 2142, 2844
BstNSI	RCATGY	6 cut(s)	997, 1108, 2059, 2489, 3578, 3993
BstSCI	CCNGG	7 cut(s)	29, 78, 1383, 1792, 2140, 2842, 3704
BstSFI	CTRYAG	3 cut(s)	1641, 1869, 4216
BstSLI	GKGCMC	4 cut(s)	505, 738, 1037, 2461
BstSNI	TACGTA	1 cut(s)	2184
BstUI	CGCG	4 cut(s)	216, 264, 298, 875
BstV2I	GAAGAC	1 cut(s)	755
BstX2I	RGATCY	7 cut(s)	838, 1273, 1387, 1405, 3060, 3079, 4555
BstXI	CCANNNNNNTGG	1 cut(s)	79
BstYI	RGATCY	7 cut(s)	838, 1273, 1387, 1405, 3060, 3079, 4555
BsuI	GTATCC	3 cut(s)	2533, 4399, 4536
BtgI	CCRYGG	1 cut(s)	296
BtgZI	GCGATG	3 cut(s)	187, 356, 890
BtrI	CACGTC	3 cut(s)	378, 2402, 4233
BtsI	GCAGTG	3 cut(s)	980, 1924, 4327
BtsIMutI	CAGTG	5 cut(s)	980, 1035, 1924, 3026, 4327
BveI	ACCTGC	2 cut(s)	1351, 3655
Cac8I	GCNNGC	7 cut(s)	300, 510, 774, 2103, 2487, 2955, 4191
CfoI	GCGC	8 cut(s)	39, 77, 87, 216, 469, 1454, 3589, 4116
Cfr10I	RCCGGY	4 cut(s)	135, 508, 533, 4450
Cfr42I	CCGCGG	1 cut(s)	299
CseI	GACGC	3 cut(s)	48, 203, 4328
CspAI	ACCGGT	1 cut(s)	135
DinI	GGCGCC	1 cut(s)	38
DraIII	CACNNNGTG	1 cut(s)	2639
Eam1104I	CTCTTC	7 cut(s)	273, 792, 1005, 1437, 1781, 2394, 3948
EarI	CTCTTC	7 cut(s)	273, 792, 1005, 1437, 1781, 2394, 3948
EciI	GGCGGA	2 cut(s)	20, 791
Ecl136II	GAGCTC	2 cut(s)	95, 2030
Eco105I	TACGTA	1 cut(s)	2184
Eco130I	CCWWGG	2 cut(s)	253, 2446
Eco24I	GRGCYC	2 cut(s)	97, 2032
Eco31I	GGTCTC	2 cut(s)	1624, 2927
Eco32I	GATATC	1 cut(s)	3962
Eco47I	GGWCC	7 cut(s)	69, 289, 1022, 1491, 2061, 2663, 4522
Eco53kI	GAGCTC	2 cut(s)	95, 2030
Eco57I	CTGAAG	5 cut(s)	1221, 1385, 2555, 2981, 3450
Eco88I	CYCGRG	2 cut(s)	737, 3503
EcoICRI	GAGCTC	2 cut(s)	95, 2030
EcoO109I	RGGNCCY	4 cut(s)	289, 1491, 2663, 3340
EcoRI	GAATTC	2 cut(s)	1879, 3895
EcoRII	CCWGG	4 cut(s)	78, 1792, 2140, 2842
EcoRV	GATATC	1 cut(s)	3962
EcoT14I	CCWWGG	2 cut(s)	253, 2446
EcoT22I	ATGCAT	2 cut(s)	2353, 2917
EcoT38I	GRGCYC	2 cut(s)	97, 2032
EgeI	GGCGCC	1 cut(s)	38
EheI	GGCGCC	1 cut(s)	38
ErhI	CCWWGG	2 cut(s)	253, 2446
FaqI	GGGAC	8 cut(s)	242, 296, 302, 1504, 2379, 3626, 3772, 4535
FauI	CCCGC	3 cut(s)	291, 300, 1339
FauNDI	CATATG	1 cut(s)	2777
FblI	GTMKAC	1 cut(s)	177
FriOI	GRGCYC	2 cut(s)	97, 2032
FspBI	CTAG	7 cut(s)	254, 1436, 2292, 2832, 3723, 3996, 4443
GlaI	GCGC	8 cut(s)	38, 76, 86, 215, 468, 1453, 3588, 4115
GsaI	CCCAGC	2 cut(s)	1533, 2255
GsuI	CTGGAG	3 cut(s)	101, 2151, 3385
HaeII	RGCGCY	3 cut(s)	40, 78, 88
HgaI	GACGC	3 cut(s)	48, 203, 4328
HhaI	GCGC	8 cut(s)	39, 77, 87, 216, 469, 1454, 3589, 4116
Hin1I	GRCGYC	5 cut(s)	37, 59, 195, 309, 4339
Hin6I	GCGC	8 cut(s)	37, 75, 85, 214, 467, 1452, 3587, 4114
HinP1I	GCGC	8 cut(s)	37, 75, 85, 214, 467, 1452, 3587, 4114
HincII	GTYRAC	4 cut(s)	178, 3205, 3946, 4467
HindII	GTYRAC	4 cut(s)	178, 3205, 3946, 4467
HindIII	AAGCTT	2 cut(s)	1826, 2099
HpaI	GTTAAC	1 cut(s)	3205
HphI	GGTGA	9 cut(s)	419, 799, 1191, 1600, 3131, 3511, 3644, 4151, 4301
Hpy166II	GTNNAC	8 cut(s)	178, 375, 3149, 3205, 3371, 3946, 4356, 4467
Hpy8I	GTNNAC	8 cut(s)	178, 375, 3149, 3205, 3371, 3946, 4356, 4467
HpyCH4IV	ACGT	7 cut(s)	309, 377, 2183, 2401, 3355, 3718, 4232
HpySE526I	ACGT	7 cut(s)	309, 377, 2183, 2401, 3355, 3718, 4232
Hsp92I	GRCGYC	5 cut(s)	37, 59, 195, 309, 4339
HspAI	GCGC	8 cut(s)	37, 75, 85, 214, 467, 1452, 3587, 4114
KasI	GGCGCC	1 cut(s)	36
KflI	GGGWCCC	1 cut(s)	289
Kpn2I	TCCGGA	1 cut(s)	1129
KpnI	GGTACC	2 cut(s)	262, 3840
KroI	GCCGGC	1 cut(s)	508
KroNI	GCCGGC	1 cut(s)	510
KspAI	GTTAAC	1 cut(s)	3205
KspI	CCGCGG	1 cut(s)	299
LguI	GCTCTTC	2 cut(s)	1437, 2394
LmnI	GCTCC	9 cut(s)	42, 50, 82, 778, 1538, 1606, 2132, 2692, 3295
MaeI	CTAG	7 cut(s)	254, 1436, 2292, 2832, 3723, 3996, 4443
MaeII	ACGT	7 cut(s)	309, 377, 2183, 2401, 3355, 3718, 4232
MbiI	CCGCTC	1 cut(s)	117
MfeI	CAATTG	2 cut(s)	3177, 4482
MflI	RGATCY	7 cut(s)	838, 1273, 1387, 1405, 3060, 3079, 4555
MhlI	GDGCHC	8 cut(s)	97, 505, 738, 1037, 1432, 2032, 2389, 2461
Mly113I	GGCGCC	1 cut(s)	37
MlyI	GAGTC	9 cut(s)	926, 1181, 1769, 1798, 2239, 2361, 3122, 3869, 3983
MmeI	TCCRAC	6 cut(s)	330, 651, 1251, 1944, 1963, 2580
Mph1103I	ATGCAT	2 cut(s)	2353, 2917
MroI	TCCGGA	1 cut(s)	1129
MroNI	GCCGGC	1 cut(s)	508
MroXI	GAANNNNTTC	3 cut(s)	3447, 3919, 4515
MslI	CAYNNNNRTG	4 cut(s)	1766, 2382, 3732, 4332
MspA1I	CMGCKG	5 cut(s)	298, 909, 2251, 2407, 4549
MspCI	CTTAAG	1 cut(s)	3400
MspR9I	CCNGG	7 cut(s)	31, 80, 1385, 1794, 2142, 2844, 3706
MunI	CAATTG	2 cut(s)	3177, 4482
Mva1269I	GAATGC	5 cut(s)	820, 1603, 1711, 2917, 3261
MvaI	CCWGG	4 cut(s)	80, 1794, 2142, 2844
MvnI	CGCG	4 cut(s)	216, 264, 298, 875
NaeI	GCCGGC	1 cut(s)	510
NarI	GGCGCC	1 cut(s)	37
NciI	CCSGG	3 cut(s)	31, 1385, 3706
NdeI	CATATG	1 cut(s)	2777
NgoMIV	GCCGGC	1 cut(s)	508
NmeAIII	GCCGAG	4 cut(s)	71, 79, 484, 1207
NmuCI	GTSAC	7 cut(s)	25, 407, 929, 978, 2198, 3359, 4486
NsiI	ATGCAT	2 cut(s)	2353, 2917
NspI	RCATGY	6 cut(s)	997, 1108, 2059, 2489, 3578, 3993
OliI	CACNNNNGTG	1 cut(s)	1766
PaeI	GCATGC	1 cut(s)	2489
PaeR7I	CTCGAG	1 cut(s)	3503
PciI	ACATGT	1 cut(s)	3574
PciSI	GCTCTTC	2 cut(s)	1437, 2394
PcsI	WCGNNNNNNNCGW	5 cut(s)	174, 183, 243, 374, 3327
PctI	GAATGC	5 cut(s)	820, 1603, 1711, 2917, 3261
PdiI	GCCGGC	1 cut(s)	510
PdmI	GAANNNNTTC	3 cut(s)	3447, 3919, 4515
PfeI	GAWTC	9 cut(s)	650, 758, 968, 2524, 3443, 3796, 3920, 4085, 4516
PflFI	GACNNNGTC	1 cut(s)	173
PflMI	CCANNNNNTGG	2 cut(s)	3853, 4402
PfoI	TCCNGGA	1 cut(s)	1383
PinAI	ACCGGT	1 cut(s)	135
Ple19I	CGATCG	1 cut(s)	24
PleI	GAGTC	9 cut(s)	926, 1181, 1769, 1797, 2239, 2361, 3122, 3869, 3982
PluTI	GGCGCC	1 cut(s)	40
PpsI	GAGTC	9 cut(s)	926, 1181, 1769, 1797, 2239, 2361, 3122, 3869, 3982
Ppu21I	YACGTR	1 cut(s)	2184
PpuMI	RGGWCCY	3 cut(s)	289, 1491, 2663
PscI	ACATGT	1 cut(s)	3574
PsiI	TTATAA	1 cut(s)	3041
Psp124BI	GAGCTC	2 cut(s)	97, 2032
Psp5II	RGGWCCY	3 cut(s)	289, 1491, 2663
Psp6I	CCWGG	4 cut(s)	78, 1792, 2140, 2842
PspFI	CCCAGC	2 cut(s)	1529, 2251
PspGI	CCWGG	4 cut(s)	78, 1792, 2140, 2842
PspPPI	RGGWCCY	3 cut(s)	289, 1491, 2663
PstI	CTGCAG	1 cut(s)	1873
PsuI	RGATCY	7 cut(s)	838, 1273, 1387, 1405, 3060, 3079, 4555
PsyI	GACNNNGTC	1 cut(s)	173
PvuI	CGATCG	1 cut(s)	24
PvuII	CAGCTG	3 cut(s)	2251, 2407, 4549
RseI	CAYNNNNRTG	4 cut(s)	1766, 2382, 3732, 4332
SacI	GAGCTC	2 cut(s)	97, 2032
SacII	CCGCGG	1 cut(s)	299
SalI	GTCGAC	1 cut(s)	176
SapI	GCTCTTC	2 cut(s)	1437, 2394
ScaI	AGTACT	1 cut(s)	3073
SchI	GAGTC	9 cut(s)	926, 1181, 1769, 1798, 2239, 2361, 3122, 3869, 3983
ScrFI	CCNGG	7 cut(s)	31, 80, 1385, 1794, 2142, 2844, 3706
SduI	GDGCHC	8 cut(s)	97, 505, 738, 1037, 1432, 2032, 2389, 2461
SfcI	CTRYAG	3 cut(s)	1641, 1869, 4216
SfoI	GGCGCC	1 cut(s)	38
Sfr274I	CTCGAG	1 cut(s)	3503
Sfr303I	CCGCGG	1 cut(s)	299
SgrBI	CCGCGG	1 cut(s)	299
SinI	GGWCC	7 cut(s)	69, 289, 1022, 1491, 2061, 2663, 4522
SlaI	CTCGAG	1 cut(s)	3503
SmiMI	CAYNNNNRTG	4 cut(s)	1766, 2382, 3732, 4332
SmlI	CTYRAG	9 cut(s)	1189, 1653, 1903, 2078, 2924, 3007, 3266, 3400, 3503
SmoI	CTYRAG	9 cut(s)	1189, 1653, 1903, 2078, 2924, 3007, 3266, 3400, 3503
SnaBI	TACGTA	1 cut(s)	2184
SphI	GCATGC	1 cut(s)	2489
SspDI	GGCGCC	1 cut(s)	36
SspMI	CTAG	7 cut(s)	254, 1436, 2292, 2832, 3723, 3996, 4443
SstI	GAGCTC	2 cut(s)	97, 2032
StyD4I	CCNGG	7 cut(s)	29, 78, 1383, 1792, 2140, 2842, 3704
StyI	CCWWGG	2 cut(s)	253, 2446
TaiI	ACGT	7 cut(s)	312, 380, 2186, 2404, 3358, 3721, 4235
TaqI	TCGA	7 cut(s)	177, 237, 368, 1170, 3330, 3504, 3893
TatI	WGTACW	7 cut(s)	1360, 1561, 3071, 3246, 4212, 4353, 4371
TfiI	GAWTC	9 cut(s)	650, 758, 968, 2524, 3443, 3796, 3920, 4085, 4516
TscAI	CASTG	5 cut(s)	987, 1042, 1924, 3033, 4334
TseFI	GTSAC	7 cut(s)	25, 407, 929, 978, 2198, 3359, 4486
Tsp45I	GTSAC	7 cut(s)	25, 407, 929, 978, 2198, 3359, 4486
TspGWI	ACGGA	6 cut(s)	508, 1556, 1811, 2572, 3140, 4373
TspRI	CASTG	5 cut(s)	987, 1042, 1924, 3033, 4334
Tth111I	GACNNNGTC	1 cut(s)	173
Van91I	CCANNNNNTGG	2 cut(s)	3853, 4402
Vha464I	CTTAAG	1 cut(s)	3400
VpaK11BI	GGWCC	7 cut(s)	69, 289, 1022, 1491, 2061, 2663, 4522
XapI	RAATTY	9 cut(s)	1514, 1580, 1879, 2800, 2869, 3408, 3688, 3895, 4473
XceI	RCATGY	6 cut(s)	997, 1108, 2059, 2489, 3578, 3993
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2055
XhoI	CTCGAG	1 cut(s)	3503
XmaJI	CCTAGG	1 cut(s)	253
XmiI	GTMKAC	1 cut(s)	177
XmnI	GAANNNNTTC	3 cut(s)	3447, 3919, 4515
XspI	CTAG	7 cut(s)	254, 1436, 2292, 2832, 3723, 3996, 4443
ZraI	GACGTC	1 cut(s)	310
ZrmI	AGTACT	1 cut(s)	3073
Zsp2I	ATGCAT	2 cut(s)	2353, 2917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.