DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 409 |
| AccB1I | GGYRCC | 1 cut(s) | 358 |
| AccII | CGCG | 1 cut(s) | 20 |
| AciI | CCGC | 1 cut(s) | 290 |
| AclWI | GGATC | 1 cut(s) | 339 |
| AcuI | CTGAAG | 3 cut(s) | 279, 443, 479 |
| AfaI | GTAC | 2 cut(s) | 420, 447 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 176 |
| AgsI | TTSAA | 1 cut(s) | 230 |
| AluBI | AGCT | 3 cut(s) | 226, 280, 347 |
| AluI | AGCT | 3 cut(s) | 226, 280, 347 |
| AlwI | GGATC | 1 cut(s) | 339 |
| AoxI | GGCC | 2 cut(s) | 33, 509 |
| ApeKI | GCWGC | 1 cut(s) | 280 |
| AspS9I | GGNCC | 2 cut(s) | 33, 167 |
| AsuC2I | CCSGG | 2 cut(s) | 37, 443 |
| AsuHPI | GGTGA | 1 cut(s) | 249 |
| AvaII | GGWCC | 1 cut(s) | 167 |
| BaeGI | GKGCMC | 1 cut(s) | 182 |
| BanI | GGYRCC | 1 cut(s) | 358 |
| BbvI | GCAGC | 1 cut(s) | 267 |
| BccI | CCATC | 2 cut(s) | 3, 113 |
| BcnI | CCSGG | 2 cut(s) | 37, 443 |
| BfmI | CTRYAG | 1 cut(s) | 402 |
| BfuAI | ACCTGC | 1 cut(s) | 409 |
| BisI | GCNGC | 2 cut(s) | 281, 291 |
| BlsI | GCNGC | 2 cut(s) | 282, 292 |
| Bme1390I | CCNGG | 2 cut(s) | 37, 443 |
| Bme18I | GGWCC | 1 cut(s) | 167 |
| BmgT120I | GGNCC | 2 cut(s) | 33, 167 |
| BmiI | GGNNCC | 2 cut(s) | 34, 360 |
| BmrFI | CCNGG | 2 cut(s) | 37, 443 |
| BmrI | ACTGGG | 1 cut(s) | 535 |
| BmsI | GCATC | 4 cut(s) | 136, 226, 277, 463 |
| BmuI | ACTGGG | 1 cut(s) | 535 |
| BpuEI | CTTGAG | 1 cut(s) | 232 |
| BpuMI | CCSGG | 2 cut(s) | 37, 443 |
| BsaBI | GATNNNNATC | 1 cut(s) | 105 |
| BsaJI | CCNNGG | 2 cut(s) | 240, 584 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 176 |
| Bse1I | ACTGG | 4 cut(s) | 31, 49, 469, 530 |
| Bse3DI | GCAATG | 1 cut(s) | 64 |
| Bse8I | GATNNNNATC | 1 cut(s) | 105 |
| BseDI | CCNNGG | 2 cut(s) | 240, 584 |
| BseGI | GGATG | 2 cut(s) | 14, 241 |
| BseJI | GATNNNNATC | 1 cut(s) | 105 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 176 |
| BseMI | GCAATG | 1 cut(s) | 64 |
| BseNI | ACTGG | 4 cut(s) | 31, 49, 469, 530 |
| BseSI | GKGCMC | 1 cut(s) | 182 |
| BseXI | GCAGC | 1 cut(s) | 267 |
| Bsh1236I | CGCG | 1 cut(s) | 20 |
| BshFI | GGCC | 2 cut(s) | 35, 511 |
| BshNI | GGYRCC | 1 cut(s) | 358 |
| BsiSI | CCGG | 2 cut(s) | 36, 442 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 176 |
| BsnI | GGCC | 2 cut(s) | 35, 511 |
| Bsp1286I | GDGCHC | 1 cut(s) | 182 |
| Bsp143I | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| Bsp68I | TCGCGA | 1 cut(s) | 20 |
| BspACI | CCGC | 1 cut(s) | 290 |
| BspANI | GGCC | 2 cut(s) | 35, 511 |
| BspFNI | CGCG | 1 cut(s) | 20 |
| BspHI | TCATGA | 1 cut(s) | 596 |
| BspLI | GGNNCC | 2 cut(s) | 34, 360 |
| BspMAI | CTGCAG | 1 cut(s) | 406 |
| BspMI | ACCTGC | 1 cut(s) | 409 |
| BspPI | GGATC | 1 cut(s) | 339 |
| BspT107I | GGYRCC | 1 cut(s) | 358 |
| BsrDI | GCAATG | 1 cut(s) | 64 |
| BsrI | ACTGG | 4 cut(s) | 31, 49, 469, 530 |
| BssECI | CCNNGG | 2 cut(s) | 240, 584 |
| BssMI | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| BssT1I | CCWWGG | 1 cut(s) | 584 |
| Bst4CI | ACNGT | 1 cut(s) | 184 |
| Bst6I | CTCTTC | 1 cut(s) | 150 |
| BstEII | GGTNACC | 1 cut(s) | 557 |
| BstF5I | GGATG | 2 cut(s) | 14, 241 |
| BstFNI | CGCG | 1 cut(s) | 20 |
| BstKTI | GATC | 5 cut(s) | 109, 334, 392, 429, 596 |
| BstMBI | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| BstNSI | RCATGY | 1 cut(s) | 142 |
| BstPI | GGTNACC | 1 cut(s) | 557 |
| BstSCI | CCNGG | 2 cut(s) | 35, 441 |
| BstSFI | CTRYAG | 1 cut(s) | 402 |
| BstSLI | GKGCMC | 1 cut(s) | 182 |
| BstUI | CGCG | 1 cut(s) | 20 |
| BstV1I | GCAGC | 1 cut(s) | 267 |
| BstX2I | RGATCY | 1 cut(s) | 331 |
| BstYI | RGATCY | 1 cut(s) | 331 |
| BsuRI | GGCC | 2 cut(s) | 35, 511 |
| BtgZI | GCGATG | 1 cut(s) | 35 |
| BtsCI | GGATG | 2 cut(s) | 14, 241 |
| BtsI | GCAGTG | 2 cut(s) | 58, 125 |
| BtsIMutI | CAGTG | 3 cut(s) | 58, 125, 180 |
| BtuMI | TCGCGA | 1 cut(s) | 20 |
| BveI | ACCTGC | 1 cut(s) | 409 |
| CciI | TCATGA | 1 cut(s) | 596 |
| Cfr13I | GGNCC | 2 cut(s) | 33, 167 |
| Csp6I | GTAC | 2 cut(s) | 419, 446 |
| CviAII | CATG | 4 cut(s) | 139, 284, 355, 597 |
| CviJI | RGCY | 8 cut(s) | 35, 40, 226, 280, 293, 347, 511, 540 |
| CviKI_1 | RGCY | 8 cut(s) | 35, 40, 226, 280, 293, 347, 511, 540 |
| CviQI | GTAC | 2 cut(s) | 419, 446 |
| DpnI | GATC | 5 cut(s) | 108, 333, 391, 428, 595 |
| DpnII | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| Eam1104I | CTCTTC | 1 cut(s) | 150 |
| EarI | CTCTTC | 1 cut(s) | 150 |
| Eco130I | CCWWGG | 1 cut(s) | 584 |
| Eco147I | AGGCCT | 1 cut(s) | 511 |
| Eco47I | GGWCC | 1 cut(s) | 167 |
| Eco57I | CTGAAG | 3 cut(s) | 279, 443, 479 |
| Eco91I | GGTNACC | 1 cut(s) | 557 |
| EcoO65I | GGTNACC | 1 cut(s) | 557 |
| EcoT14I | CCWWGG | 1 cut(s) | 584 |
| ErhI | CCWWGG | 1 cut(s) | 584 |
| FaeI | CATG | 4 cut(s) | 142, 287, 358, 600 |
| FatI | CATG | 4 cut(s) | 138, 283, 354, 596 |
| Fnu4HI | GCNGC | 2 cut(s) | 281, 291 |
| FokI | GGATG | 2 cut(s) | 21, 248 |
| Fsp4HI | GCNGC | 2 cut(s) | 281, 291 |
| GluI | GCNGC | 2 cut(s) | 281, 291 |
| HaeIII | GGCC | 2 cut(s) | 35, 511 |
| HapII | CCGG | 2 cut(s) | 36, 442 |
| Hin1II | CATG | 4 cut(s) | 142, 287, 358, 600 |
| HinfI | GANTC | 3 cut(s) | 77, 113, 245 |
| HpaII | CCGG | 2 cut(s) | 36, 442 |
| HphI | GGTGA | 1 cut(s) | 249 |
| Hpy166II | GTNNAC | 1 cut(s) | 448 |
| Hpy188I | TCNGA | 4 cut(s) | 118, 298, 394, 498 |
| Hpy188III | TCNNGA | 4 cut(s) | 19, 110, 367, 597 |
| Hpy8I | GTNNAC | 1 cut(s) | 448 |
| Hpy99I | CGWCG | 1 cut(s) | 458 |
| HpyAV | CCTTC | 3 cut(s) | 7, 83, 193 |
| HpyCH4III | ACNGT | 1 cut(s) | 184 |
| HpyCH4V | TGCA | 3 cut(s) | 283, 404, 476 |
| Hsp92II | CATG | 4 cut(s) | 142, 287, 358, 600 |
| Kzo9I | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| Lsp1109I | GCAGC | 1 cut(s) | 267 |
| LweI | GCATC | 4 cut(s) | 136, 226, 277, 463 |
| MaeIII | GTNAC | 3 cut(s) | 74, 123, 557 |
| MalI | GATC | 5 cut(s) | 108, 333, 391, 428, 595 |
| MboI | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| MboII | GAAGA | 3 cut(s) | 167, 436, 485 |
| MflI | RGATCY | 1 cut(s) | 331 |
| MhlI | GDGCHC | 1 cut(s) | 182 |
| MluCI | AATT | 5 cut(s) | 81, 299, 311, 412, 430 |
| MlyI | GAGTC | 2 cut(s) | 71, 239 |
| MmeI | TCCRAC | 1 cut(s) | 309 |
| MnlI | CCTC | 4 cut(s) | 58, 235, 363, 563 |
| MseI | TTAA | 3 cut(s) | 204, 302, 542 |
| MspI | CCGG | 2 cut(s) | 36, 442 |
| MspR9I | CCNGG | 2 cut(s) | 37, 443 |
| MvnI | CGCG | 1 cut(s) | 20 |
| NciI | CCSGG | 2 cut(s) | 37, 443 |
| NdeII | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| NlaIII | CATG | 4 cut(s) | 142, 287, 358, 600 |
| NlaIV | GGNNCC | 2 cut(s) | 34, 360 |
| NmeAIII | GCCGAG | 1 cut(s) | 265 |
| NmuCI | GTSAC | 2 cut(s) | 74, 123 |
| NruI | TCGCGA | 1 cut(s) | 20 |
| NspI | RCATGY | 1 cut(s) | 142 |
| PagI | TCATGA | 1 cut(s) | 596 |
| PceI | AGGCCT | 1 cut(s) | 511 |
| PfeI | GAWTC | 1 cut(s) | 113 |
| PkrI | GCNGC | 2 cut(s) | 282, 292 |
| PleI | GAGTC | 2 cut(s) | 71, 239 |
| PpsI | GAGTC | 2 cut(s) | 71, 239 |
| PspEI | GGTNACC | 1 cut(s) | 557 |
| PspN4I | GGNNCC | 2 cut(s) | 34, 360 |
| PspPI | GGNCC | 2 cut(s) | 33, 167 |
| PstI | CTGCAG | 1 cut(s) | 406 |
| PsuI | RGATCY | 1 cut(s) | 331 |
| RruI | TCGCGA | 1 cut(s) | 20 |
| RsaI | GTAC | 2 cut(s) | 420, 447 |
| RsaNI | GTAC | 2 cut(s) | 419, 446 |
| SaqAI | TTAA | 3 cut(s) | 204, 302, 542 |
| SatI | GCNGC | 2 cut(s) | 281, 291 |
| Sau3AI | GATC | 5 cut(s) | 106, 331, 389, 426, 593 |
| Sau96I | GGNCC | 2 cut(s) | 33, 167 |
| SbfI | CCTGCAGG | 1 cut(s) | 406 |
| SchI | GAGTC | 2 cut(s) | 71, 239 |
| ScrFI | CCNGG | 2 cut(s) | 37, 443 |
| SdaI | CCTGCAGG | 1 cut(s) | 406 |
| SduI | GDGCHC | 1 cut(s) | 182 |
| SetI | ASST | 9 cut(s) | 18, 69, 172, 204, 228, 282, 349, 364, 403 |
| SfaNI | GCATC | 4 cut(s) | 136, 226, 277, 463 |
| SfcI | CTRYAG | 1 cut(s) | 402 |
| SinI | GGWCC | 1 cut(s) | 167 |
| SmlI | CTYRAG | 1 cut(s) | 247 |
| SmoI | CTYRAG | 1 cut(s) | 247 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 406 |
| Sse9I | AATT | 5 cut(s) | 81, 299, 311, 412, 430 |
| SseBI | AGGCCT | 1 cut(s) | 511 |
| SsiI | CCGC | 1 cut(s) | 290 |
| StuI | AGGCCT | 1 cut(s) | 511 |
| StyD4I | CCNGG | 2 cut(s) | 35, 441 |
| StyI | CCWWGG | 1 cut(s) | 584 |
| TaaI | ACNGT | 1 cut(s) | 184 |
| TasI | AATT | 5 cut(s) | 81, 299, 311, 412, 430 |
| TatI | WGTACW | 1 cut(s) | 418 |
| TauI | GCSGC | 1 cut(s) | 293 |
| TfiI | GAWTC | 1 cut(s) | 113 |
| Tru1I | TTAA | 3 cut(s) | 204, 302, 542 |
| Tru9I | TTAA | 3 cut(s) | 204, 302, 542 |
| TscAI | CASTG | 3 cut(s) | 58, 132, 187 |
| TseFI | GTSAC | 2 cut(s) | 74, 123 |
| TseI | GCWGC | 1 cut(s) | 280 |
| Tsp45I | GTSAC | 2 cut(s) | 74, 123 |
| TspDTI | ATGAA | 2 cut(s) | 207, 324 |
| TspRI | CASTG | 3 cut(s) | 58, 132, 187 |
| VpaK11BI | GGWCC | 1 cut(s) | 167 |
| XceI | RCATGY | 1 cut(s) | 142 |