DNA polymerase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DNA_pol_alpha_N | PF12254 | 2 - 46 | 2.1e-13 | DNA polymerase alpha subunit p180 N terminal |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 566 |
| Acc65I | GGTACC | 1 cut(s) | 108 |
| AccB1I | GGYRCC | 1 cut(s) | 108 |
| AccI | GTMKAC | 1 cut(s) | 27 |
| AccII | CGCG | 2 cut(s) | 114, 244 |
| AciI | CCGC | 3 cut(s) | 112, 242, 244 |
| AfaI | GTAC | 2 cut(s) | 41, 110 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| AgsI | TTSAA | 1 cut(s) | 466 |
| AjnI | CCWGG | 1 cut(s) | 582 |
| Alw26I | GTCTC | 1 cut(s) | 427 |
| AoxI | GGCC | 4 cut(s) | 100, 188, 245, 357 |
| ApeKI | GCWGC | 2 cut(s) | 281, 474 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 473, 505 |
| Asp718I | GGTACC | 1 cut(s) | 108 |
| AspA2I | CCTAGG | 1 cut(s) | 103 |
| AspLEI | GCGC | 1 cut(s) | 412 |
| AspS9I | GGNCC | 3 cut(s) | 138, 189, 246 |
| AsuHPI | GGTGA | 1 cut(s) | 362 |
| AvaII | GGWCC | 1 cut(s) | 138 |
| AvrII | CCTAGG | 1 cut(s) | 103 |
| BaeGI | GKGCMC | 1 cut(s) | 448 |
| BanI | GGYRCC | 1 cut(s) | 108 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 31 |
| BbsI | GAAGAC | 2 cut(s) | 209, 564 |
| BbvI | GCAGC | 2 cut(s) | 293, 486 |
| BccI | CCATC | 4 cut(s) | 56, 283, 341, 383 |
| BceAI | ACGGC | 4 cut(s) | 115, 288, 294, 551 |
| BciT130I | CCWGG | 1 cut(s) | 584 |
| BcoDI | GTCTC | 1 cut(s) | 427 |
| BfaI | CTAG | 2 cut(s) | 104, 510 |
| BisI | GCNGC | 4 cut(s) | 242, 245, 282, 475 |
| BlnI | CCTAGG | 1 cut(s) | 103 |
| BlsI | GCNGC | 4 cut(s) | 243, 246, 283, 476 |
| Bme1390I | CCNGG | 1 cut(s) | 584 |
| Bme18I | GGWCC | 1 cut(s) | 138 |
| BmgT120I | GGNCC | 3 cut(s) | 138, 189, 246 |
| BmiI | GGNNCC | 4 cut(s) | 110, 140, 240, 581 |
| BmrFI | CCNGG | 1 cut(s) | 584 |
| BpiI | GAAGAC | 2 cut(s) | 209, 564 |
| BsaJI | CCNNGG | 3 cut(s) | 103, 145, 242 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| Bse118I | RCCGGY | 1 cut(s) | 451 |
| Bse1I | ACTGG | 1 cut(s) | 140 |
| Bse3DI | GCAATG | 1 cut(s) | 520 |
| BseBI | CCWGG | 1 cut(s) | 584 |
| BseDI | CCNNGG | 3 cut(s) | 103, 145, 242 |
| BseGI | GGATG | 4 cut(s) | 97, 172, 352, 394 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| BseMI | GCAATG | 1 cut(s) | 520 |
| BseMII | CTCAG | 2 cut(s) | 393, 552 |
| BseNI | ACTGG | 1 cut(s) | 140 |
| BseRI | GAGGAG | 5 cut(s) | 83, 211, 454, 472, 566 |
| BseSI | GKGCMC | 1 cut(s) | 448 |
| BseXI | GCAGC | 2 cut(s) | 293, 486 |
| Bsh1236I | CGCG | 2 cut(s) | 114, 244 |
| Bsh1285I | CGRYCG | 1 cut(s) | 27 |
| BshFI | GGCC | 4 cut(s) | 102, 190, 247, 359 |
| BshNI | GGYRCC | 1 cut(s) | 108 |
| BsiEI | CGRYCG | 1 cut(s) | 27 |
| BsiSI | CCGG | 2 cut(s) | 413, 452 |
| BslFI | GGGAC | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 147, 198 |
| BsmAI | GTCTC | 1 cut(s) | 427 |
| BsmBI | CGTCTC | 1 cut(s) | 427 |
| BsmFI | GGGAC | 1 cut(s) | 92 |
| BsnI | GGCC | 4 cut(s) | 102, 190, 247, 359 |
| Bsp1286I | GDGCHC | 1 cut(s) | 448 |
| Bsp143I | GATC | 1 cut(s) | 12 |
| BspACI | CCGC | 3 cut(s) | 112, 242, 244 |
| BspANI | GGCC | 4 cut(s) | 102, 190, 247, 359 |
| BspCNI | CTCAG | 2 cut(s) | 394, 551 |
| BspFNI | CGCG | 2 cut(s) | 114, 244 |
| BspLI | GGNNCC | 4 cut(s) | 110, 140, 240, 581 |
| BspT107I | GGYRCC | 1 cut(s) | 108 |
| BsrDI | GCAATG | 1 cut(s) | 520 |
| BsrFI | RCCGGY | 1 cut(s) | 451 |
| BsrI | ACTGG | 1 cut(s) | 140 |
| BssAI | RCCGGY | 1 cut(s) | 451 |
| BssECI | CCNNGG | 3 cut(s) | 103, 145, 242 |
| BssMI | GATC | 1 cut(s) | 12 |
| BssT1I | CCWWGG | 1 cut(s) | 103 |
| Bst2UI | CCWGG | 1 cut(s) | 584 |
| Bst4CI | ACNGT | 2 cut(s) | 25, 208 |
| Bst6I | CTCTTC | 3 cut(s) | 30, 123, 537 |
| BstC8I | GCNNGC | 1 cut(s) | 453 |
| BstDEI | CTNAG | 2 cut(s) | 402, 538 |
| BstDSI | CCRYGG | 2 cut(s) | 145, 242 |
| BstF5I | GGATG | 4 cut(s) | 97, 172, 352, 394 |
| BstFNI | CGCG | 2 cut(s) | 114, 244 |
| BstHHI | GCGC | 1 cut(s) | 412 |
| BstKTI | GATC | 1 cut(s) | 15 |
| BstMAI | GTCTC | 1 cut(s) | 427 |
| BstMBI | GATC | 1 cut(s) | 12 |
| BstMCI | CGRYCG | 1 cut(s) | 27 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 253, 281, 407, 452 |
| BstNI | CCWGG | 1 cut(s) | 584 |
| BstSCI | CCNGG | 1 cut(s) | 582 |
| BstSLI | GKGCMC | 1 cut(s) | 448 |
| BstUI | CGCG | 2 cut(s) | 114, 244 |
| BstV1I | GCAGC | 2 cut(s) | 293, 486 |
| BstV2I | GAAGAC | 2 cut(s) | 209, 564 |
| BsuRI | GGCC | 4 cut(s) | 102, 190, 247, 359 |
| BtgI | CCRYGG | 2 cut(s) | 145, 242 |
| BtgZI | GCGATG | 2 cut(s) | 37, 299 |
| BtsCI | GGATG | 4 cut(s) | 97, 172, 352, 394 |
| Cac8I | GCNNGC | 1 cut(s) | 453 |
| CfoI | GCGC | 1 cut(s) | 412 |
| Cfr10I | RCCGGY | 1 cut(s) | 451 |
| Cfr13I | GGNCC | 3 cut(s) | 138, 189, 246 |
| Cfr42I | CCGCGG | 1 cut(s) | 245 |
| Csp6I | GTAC | 2 cut(s) | 40, 109 |
| CviQI | GTAC | 2 cut(s) | 40, 109 |
| DdeI | CTNAG | 2 cut(s) | 402, 538 |
| DpnI | GATC | 1 cut(s) | 14 |
| DpnII | GATC | 1 cut(s) | 12 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 566 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 566 |
| Eam1104I | CTCTTC | 3 cut(s) | 30, 123, 537 |
| EarI | CTCTTC | 3 cut(s) | 30, 123, 537 |
| Eco130I | CCWWGG | 1 cut(s) | 103 |
| Eco47I | GGWCC | 1 cut(s) | 138 |
| EcoRII | CCWGG | 1 cut(s) | 582 |
| EcoT14I | CCWWGG | 1 cut(s) | 103 |
| ErhI | CCWWGG | 1 cut(s) | 103 |
| Esp3I | CGTCTC | 1 cut(s) | 427 |
| FaiI | YATR | 3 cut(s) | 63, 185, 471 |
| FaqI | GGGAC | 1 cut(s) | 92 |
| FblI | GTMKAC | 1 cut(s) | 27 |
| Fnu4HI | GCNGC | 4 cut(s) | 242, 245, 282, 475 |
| FokI | GGATG | 4 cut(s) | 104, 179, 359, 401 |
| Fsp4HI | GCNGC | 4 cut(s) | 242, 245, 282, 475 |
| FspBI | CTAG | 2 cut(s) | 104, 510 |
| GlaI | GCGC | 1 cut(s) | 411 |
| GluI | GCNGC | 4 cut(s) | 242, 245, 282, 475 |
| HaeIII | GGCC | 4 cut(s) | 102, 190, 247, 359 |
| HapII | CCGG | 2 cut(s) | 413, 452 |
| HhaI | GCGC | 1 cut(s) | 412 |
| Hin6I | GCGC | 1 cut(s) | 410 |
| HinP1I | GCGC | 1 cut(s) | 410 |
| HincII | GTYRAC | 1 cut(s) | 28 |
| HindII | GTYRAC | 1 cut(s) | 28 |
| HinfI | GANTC | 3 cut(s) | 170, 494, 529 |
| HpaII | CCGG | 2 cut(s) | 413, 452 |
| HphI | GGTGA | 1 cut(s) | 362 |
| Hpy166II | GTNNAC | 2 cut(s) | 28, 318 |
| Hpy188I | TCNGA | 4 cut(s) | 156, 166, 175, 541 |
| Hpy188III | TCNNGA | 1 cut(s) | 526 |
| Hpy8I | GTNNAC | 2 cut(s) | 28, 318 |
| Hpy99I | CGWCG | 9 cut(s) | 32, 101, 119, 122, 313, 482, 485, 503, 572 |
| HpyAV | CCTTC | 4 cut(s) | 211, 217, 223, 229 |
| HpyCH4III | ACNGT | 2 cut(s) | 25, 208 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 253, 281, 407, 452 |
| HpyF3I | CTNAG | 2 cut(s) | 402, 538 |
| HspAI | GCGC | 1 cut(s) | 410 |
| KpnI | GGTACC | 1 cut(s) | 112 |
| KroI | GCCGGC | 1 cut(s) | 451 |
| KroNI | GCCGGC | 1 cut(s) | 453 |
| KspI | CCGCGG | 1 cut(s) | 245 |
| Kzo9I | GATC | 1 cut(s) | 12 |
| LmnI | GCTCC | 1 cut(s) | 238 |
| LpnPI | CCDG | 4 cut(s) | 121, 426, 465, 569 |
| Lsp1109I | GCAGC | 2 cut(s) | 293, 486 |
| MaeI | CTAG | 2 cut(s) | 104, 510 |
| MaeIII | GTNAC | 2 cut(s) | 264, 350 |
| MalI | GATC | 1 cut(s) | 14 |
| MboI | GATC | 1 cut(s) | 12 |
| MboII | GAAGA | 6 cut(s) | 47, 140, 214, 226, 554, 569 |
| MhlI | GDGCHC | 1 cut(s) | 448 |
| MluCI | AATT | 1 cut(s) | 259 |
| MroNI | GCCGGC | 1 cut(s) | 451 |
| MseI | TTAA | 1 cut(s) | 333 |
| MspA1I | CMGCKG | 1 cut(s) | 244 |
| MspI | CCGG | 2 cut(s) | 413, 452 |
| MspR9I | CCNGG | 1 cut(s) | 584 |
| MvaI | CCWGG | 1 cut(s) | 584 |
| MvnI | CGCG | 2 cut(s) | 114, 244 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 253, 281, 407, 452 |
| NaeI | GCCGGC | 1 cut(s) | 453 |
| NdeII | GATC | 1 cut(s) | 12 |
| NgoMIV | GCCGGC | 1 cut(s) | 451 |
| NlaIV | GGNNCC | 4 cut(s) | 110, 140, 240, 581 |
| NmuCI | GTSAC | 2 cut(s) | 264, 350 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 24, 93, 489, 498 |
| PdiI | GCCGGC | 1 cut(s) | 453 |
| PfeI | GAWTC | 3 cut(s) | 170, 494, 529 |
| PflFI | GACNNNGTC | 1 cut(s) | 23 |
| PkrI | GCNGC | 4 cut(s) | 243, 246, 283, 476 |
| Psp6I | CCWGG | 1 cut(s) | 582 |
| PspGI | CCWGG | 1 cut(s) | 582 |
| PspN4I | GGNNCC | 4 cut(s) | 110, 140, 240, 581 |
| PspPI | GGNCC | 3 cut(s) | 138, 189, 246 |
| PsyI | GACNNNGTC | 1 cut(s) | 23 |
| RsaI | GTAC | 2 cut(s) | 41, 110 |
| RsaNI | GTAC | 2 cut(s) | 40, 109 |
| SacII | CCGCGG | 1 cut(s) | 245 |
| SalI | GTCGAC | 1 cut(s) | 26 |
| SaqAI | TTAA | 1 cut(s) | 333 |
| SatI | GCNGC | 4 cut(s) | 242, 245, 282, 475 |
| Sau3AI | GATC | 1 cut(s) | 12 |
| Sau96I | GGNCC | 3 cut(s) | 138, 189, 246 |
| ScrFI | CCNGG | 1 cut(s) | 584 |
| SduI | GDGCHC | 1 cut(s) | 448 |
| SetI | ASST | 1 cut(s) | 581 |
| Sfr303I | CCGCGG | 1 cut(s) | 245 |
| SgrBI | CCGCGG | 1 cut(s) | 245 |
| SinI | GGWCC | 1 cut(s) | 138 |
| Sse9I | AATT | 1 cut(s) | 259 |
| SsiI | CCGC | 3 cut(s) | 112, 242, 244 |
| SspMI | CTAG | 2 cut(s) | 104, 510 |
| StyD4I | CCNGG | 1 cut(s) | 582 |
| StyI | CCWWGG | 1 cut(s) | 103 |
| TaaI | ACNGT | 2 cut(s) | 25, 208 |
| TaqI | TCGA | 4 cut(s) | 27, 87, 311, 492 |
| TasI | AATT | 1 cut(s) | 259 |
| TauI | GCSGC | 2 cut(s) | 244, 247 |
| TfiI | GAWTC | 3 cut(s) | 170, 494, 529 |
| Tru1I | TTAA | 1 cut(s) | 333 |
| Tru9I | TTAA | 1 cut(s) | 333 |
| TseFI | GTSAC | 2 cut(s) | 264, 350 |
| TseI | GCWGC | 2 cut(s) | 281, 474 |
| Tsp45I | GTSAC | 2 cut(s) | 264, 350 |
| TspDTI | ATGAA | 2 cut(s) | 73, 183 |
| TspGWI | ACGGA | 2 cut(s) | 451, 487 |
| Tth111I | GACNNNGTC | 1 cut(s) | 23 |
| VpaK11BI | GGWCC | 1 cut(s) | 138 |
| XmaJI | CCTAGG | 1 cut(s) | 103 |
| XmiI | GTMKAC | 1 cut(s) | 27 |
| XspI | CTAG | 2 cut(s) | 104, 510 |