Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Forward (+)

19387680 .. 19388792

Gene Structure 1113 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017638

Sequence Viewer

CDS Protein Promoter (2k)

Length: 600 bp

ATGACGAGCAACCTGGAGTGCCTCCGAGCTCTCTGGCAAGGCGGCCGCCGCTCTGAAAACTCCGACGACTGGTTCCAGATCAAGATGGAGAAGCCGATCTACGACACGGTTGACGAGGAAGAGTACGACACCATCATCGCCAAGCAGAGCGAGGAGTTCGGGGACTTCATCGAGGAAGACGACTGCCTAGGGTACCGCGACGACGGCGAGGAAGAGGACTGGACCTGCCGCGGGCTTCCGACGTCGTCGAATGAATCGAAGGGCGATATGAGGCCCAGGAGGAGGAAGACGGTGGAGAAGAAGGATAAGGAGAAGGAGCCGCGGCCCAAAAAGCGCAATTCGTCGCTTACGGTGACGGCAGCGATGATGGGGAAGCAGAGGCTTTCGTCGGTGTTTAATAAAAGTAGGGATGGTCACAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCCGAGTTTGCGCCGGACGAGGCTGATAGAGAGAGACGGAGGAGGGTACAGCCGGCGAGGAGTTTTGTTCCGATTACTGGAGTTAAGACCATGGGCCTAGGCCTGGGCCTGAGACTGGGCTCTTGGGCTTGGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

200

Amino Acids

22.8

Weight (kDa)

5.5

Isoelectric Point (pI)

71.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	5 - 74	5.8e-17	DNA polymerase alpha subunit p180 N terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	245
Acc36I	ACCTGC	1 cut(s)	233
Acc65I	GGTACC	1 cut(s)	192
AccB1I	GGYRCC	1 cut(s)	192
AccBSI	CCGCTC	1 cut(s)	51
AccII	CGCG	3 cut(s)	198, 231, 322
AciI	CCGC	8 cut(s)	42, 46, 49, 196, 229, 231, 320, 322
AcoI	YGGCCR	1 cut(s)	43
AcyI	GRCGYC	1 cut(s)	242
AfaI	GTAC	3 cut(s)	125, 194, 510
AfiI	CCNNNNNNNGG	6 cut(s)	69, 231, 282, 539, 565, 577
AjnI	CCWGG	3 cut(s)	12, 275, 564
AluBI	AGCT	1 cut(s)	29
AluI	AGCT	1 cut(s)	29
Alw21I	GWGCWC	1 cut(s)	31
Alw26I	GTCTC	2 cut(s)	490, 568
AoxI	GGCC	7 cut(s)	43, 272, 323, 420, 556, 562, 568
ApeKI	GCWGC	1 cut(s)	359
Asp718I	GGTACC	1 cut(s)	192
AspA2I	CCTAGG	2 cut(s)	187, 559
AspLEI	GCGC	2 cut(s)	336, 475
AspS9I	GGNCC	5 cut(s)	222, 273, 324, 556, 568
AsuHPI	GGTGA	1 cut(s)	364
AvaII	GGWCC	1 cut(s)	222
AvrII	CCTAGG	2 cut(s)	187, 559
BanI	GGYRCC	1 cut(s)	192
BanII	GRGCYC	2 cut(s)	31, 584
BarI	GAAGNNNNNNTAC	2 cut(s)	83, 115
BbsI	GAAGAC	2 cut(s)	183, 293
Bbv12I	GWGCWC	1 cut(s)	31
BbvI	GCAGC	1 cut(s)	371
BccI	CCATC	4 cut(s)	79, 140, 361, 404
BceAI	ACGGC	2 cut(s)	220, 372
BciT130I	CCWGG	3 cut(s)	14, 277, 566
BcoDI	GTCTC	2 cut(s)	490, 568
BfaI	CTAG	2 cut(s)	188, 560
BfuAI	ACCTGC	1 cut(s)	233
BisI	GCNGC	7 cut(s)	43, 46, 49, 229, 320, 323, 360
BlnI	CCTAGG	2 cut(s)	187, 559
BlsI	GCNGC	7 cut(s)	44, 47, 50, 230, 321, 324, 361
Bme1390I	CCNGG	3 cut(s)	14, 277, 566
Bme18I	GGWCC	1 cut(s)	222
BmgT120I	GGNCC	5 cut(s)	222, 273, 324, 556, 568
BmiI	GGNNCC	3 cut(s)	74, 194, 318
BmrFI	CCNGG	3 cut(s)	14, 277, 566
BmrI	ACTGGG	1 cut(s)	587
BmuI	ACTGGG	1 cut(s)	587
BpiI	GAAGAC	2 cut(s)	183, 293
BpmI	CTGGAG	2 cut(s)	35, 561
BsaHI	GRCGYC	1 cut(s)	242
BsaJI	CCNNGG	7 cut(s)	187, 229, 275, 320, 552, 559, 565
Bsc4I	CCNNNNNNNGG	6 cut(s)	69, 231, 282, 539, 565, 577
Bse118I	RCCGGY	1 cut(s)	514
Bse1I	ACTGG	4 cut(s)	74, 224, 544, 582
BseBI	CCWGG	3 cut(s)	14, 277, 566
BseDI	CCNNGG	7 cut(s)	187, 229, 275, 320, 552, 559, 565
BseGI	GGATG	2 cut(s)	415, 457
BseLI	CCNNNNNNNGG	6 cut(s)	69, 231, 282, 539, 565, 577
BseMII	CTCAG	1 cut(s)	563
BseNI	ACTGG	4 cut(s)	74, 224, 544, 582
BseRI	GAGGAG	4 cut(s)	167, 295, 517, 535
BseX3I	CGGCCG	1 cut(s)	43
BseXI	GCAGC	1 cut(s)	371
Bsh1236I	CGCG	3 cut(s)	198, 231, 322
Bsh1285I	CGRYCG	1 cut(s)	46
BshFI	GGCC	7 cut(s)	45, 274, 325, 422, 558, 564, 570
BshNI	GGYRCC	1 cut(s)	192
BsiEI	CGRYCG	1 cut(s)	46
BsiHKAI	GWGCWC	1 cut(s)	31
BsiSI	CCGG	2 cut(s)	476, 515
BslFI	GGGAC	1 cut(s)	176
BslI	CCNNNNNNNGG	6 cut(s)	69, 231, 282, 539, 565, 577
BsmAI	GTCTC	2 cut(s)	490, 568
BsmBI	CGTCTC	1 cut(s)	490
BsmFI	GGGAC	1 cut(s)	176
BsnI	GGCC	7 cut(s)	45, 274, 325, 422, 558, 564, 570
Bsp1286I	GDGCHC	2 cut(s)	31, 584
Bsp143I	GATC	2 cut(s)	78, 96
Bsp19I	CCATGG	1 cut(s)	552
BspACI	CCGC	8 cut(s)	42, 46, 49, 196, 229, 231, 320, 322
BspANI	GGCC	7 cut(s)	45, 274, 325, 422, 558, 564, 570
BspCNI	CTCAG	1 cut(s)	564
BspFNI	CGCG	3 cut(s)	198, 231, 322
BspLI	GGNNCC	3 cut(s)	74, 194, 318
BspMI	ACCTGC	1 cut(s)	233
BspT107I	GGYRCC	1 cut(s)	192
BsrBI	CCGCTC	1 cut(s)	51
BsrFI	RCCGGY	1 cut(s)	514
BsrI	ACTGG	4 cut(s)	74, 224, 544, 582
BssAI	RCCGGY	1 cut(s)	514
BssECI	CCNNGG	7 cut(s)	187, 229, 275, 320, 552, 559, 565
BssMI	GATC	2 cut(s)	78, 96
BssNI	GRCGYC	1 cut(s)	242
BssT1I	CCWWGG	3 cut(s)	187, 552, 559
Bst2UI	CCWGG	3 cut(s)	14, 277, 566
Bst4CI	ACNGT	3 cut(s)	109, 292, 352
Bst6I	CTCTTC	2 cut(s)	114, 207
BstACI	GRCGYC	1 cut(s)	242
BstC8I	GCNNGC	2 cut(s)	233, 516
BstDEI	CTNAG	2 cut(s)	572, 597
BstDSI	CCRYGG	3 cut(s)	229, 320, 552
BstF5I	GGATG	2 cut(s)	415, 457
BstFNI	CGCG	3 cut(s)	198, 231, 322
BstHHI	GCGC	2 cut(s)	336, 475
BstKTI	GATC	2 cut(s)	81, 99
BstMAI	GTCTC	2 cut(s)	490, 568
BstMBI	GATC	2 cut(s)	78, 96
BstMCI	CGRYCG	1 cut(s)	46
BstMWI	GCNNNNNNNGC	4 cut(s)	48, 204, 331, 470
BstNI	CCWGG	3 cut(s)	14, 277, 566
BstSCI	CCNGG	3 cut(s)	12, 275, 564
BstUI	CGCG	3 cut(s)	198, 231, 322
BstV1I	GCAGC	1 cut(s)	371
BstV2I	GAAGAC	2 cut(s)	183, 293
BstZI	CGGCCG	1 cut(s)	43
BsuRI	GGCC	7 cut(s)	45, 274, 325, 422, 558, 564, 570
BtgI	CCRYGG	3 cut(s)	229, 320, 552
BtgZI	GCGATG	2 cut(s)	121, 377
BtsCI	GGATG	2 cut(s)	415, 457
BveI	ACCTGC	1 cut(s)	233
Cac8I	GCNNGC	2 cut(s)	233, 516
CciNI	GCGGCCGC	1 cut(s)	43
CfoI	GCGC	2 cut(s)	336, 475
Cfr10I	RCCGGY	1 cut(s)	514
Cfr13I	GGNCC	5 cut(s)	222, 273, 324, 556, 568
Cfr42I	CCGCGG	2 cut(s)	232, 323
Csp6I	GTAC	3 cut(s)	124, 193, 509
CviAII	CATG	1 cut(s)	553
CviQI	GTAC	3 cut(s)	124, 193, 509
DdeI	CTNAG	2 cut(s)	572, 597
DpnI	GATC	2 cut(s)	80, 98
DpnII	GATC	2 cut(s)	78, 96
EaeI	YGGCCR	1 cut(s)	43
EagI	CGGCCG	1 cut(s)	43
Eam1104I	CTCTTC	2 cut(s)	114, 207
EarI	CTCTTC	2 cut(s)	114, 207
Ecl136II	GAGCTC	1 cut(s)	29
EclXI	CGGCCG	1 cut(s)	43
Eco130I	CCWWGG	3 cut(s)	187, 552, 559
Eco147I	AGGCCT	1 cut(s)	564
Eco24I	GRGCYC	2 cut(s)	31, 584
Eco47I	GGWCC	1 cut(s)	222
Eco52I	CGGCCG	1 cut(s)	43
Eco53kI	GAGCTC	1 cut(s)	29
EcoICRI	GAGCTC	1 cut(s)	29
EcoRII	CCWGG	3 cut(s)	12, 275, 564
EcoT14I	CCWWGG	3 cut(s)	187, 552, 559
EcoT38I	GRGCYC	2 cut(s)	31, 584
ErhI	CCWWGG	3 cut(s)	187, 552, 559
Esp3I	CGTCTC	1 cut(s)	490
FaeI	CATG	1 cut(s)	556
FaiI	YATR	2 cut(s)	269, 554
FaqI	GGGAC	1 cut(s)	176
FatI	CATG	1 cut(s)	552
FauI	CCCGC	1 cut(s)	224
Fnu4HI	GCNGC	7 cut(s)	43, 46, 49, 229, 320, 323, 360
FokI	GGATG	2 cut(s)	422, 464
FriOI	GRGCYC	2 cut(s)	31, 584
Fsp4HI	GCNGC	7 cut(s)	43, 46, 49, 229, 320, 323, 360
FspBI	CTAG	2 cut(s)	188, 560
GlaI	GCGC	2 cut(s)	335, 474
GluI	GCNGC	7 cut(s)	43, 46, 49, 229, 320, 323, 360
GsuI	CTGGAG	2 cut(s)	35, 561
HaeIII	GGCC	7 cut(s)	45, 274, 325, 422, 558, 564, 570
HapII	CCGG	2 cut(s)	476, 515
HhaI	GCGC	2 cut(s)	336, 475
Hin1I	GRCGYC	1 cut(s)	242
Hin1II	CATG	1 cut(s)	556
Hin6I	GCGC	2 cut(s)	334, 473
HinP1I	GCGC	2 cut(s)	334, 473
HincII	GTYRAC	1 cut(s)	112
HindII	GTYRAC	1 cut(s)	112
HinfI	GANTC	1 cut(s)	254
HpaII	CCGG	2 cut(s)	476, 515
HphI	GGTGA	1 cut(s)	364
Hpy166II	GTNNAC	1 cut(s)	112
Hpy188I	TCNGA	5 cut(s)	26, 55, 64, 240, 534
Hpy188III	TCNNGA	2 cut(s)	76, 82
Hpy8I	GTNNAC	1 cut(s)	112
Hpy99I	CGWCG	8 cut(s)	68, 203, 206, 244, 247, 250, 346, 391
HpyAV	CCTTC	3 cut(s)	253, 295, 307
HpyCH4III	ACNGT	3 cut(s)	109, 292, 352
HpyCH4IV	ACGT	1 cut(s)	242
HpyF10VI	GCNNNNNNNGC	4 cut(s)	48, 204, 331, 470
HpyF3I	CTNAG	2 cut(s)	572, 597
HpySE526I	ACGT	1 cut(s)	242
Hsp92I	GRCGYC	1 cut(s)	242
Hsp92II	CATG	1 cut(s)	556
HspAI	GCGC	2 cut(s)	334, 473
KpnI	GGTACC	1 cut(s)	196
KroI	GCCGGC	1 cut(s)	514
KroNI	GCCGGC	1 cut(s)	516
KspI	CCGCGG	2 cut(s)	232, 323
Kzo9I	GATC	2 cut(s)	78, 96
LmnI	GCTCC	1 cut(s)	316
Lsp1109I	GCAGC	1 cut(s)	371
MaeI	CTAG	2 cut(s)	188, 560
MaeII	ACGT	1 cut(s)	242
MaeIII	GTNAC	2 cut(s)	352, 413
MalI	GATC	2 cut(s)	80, 98
MbiI	CCGCTC	1 cut(s)	51
MboI	GATC	2 cut(s)	78, 96
MboII	GAAGA	5 cut(s)	131, 188, 224, 298, 310
MhlI	GDGCHC	2 cut(s)	31, 584
MluCI	AATT	1 cut(s)	337
MmeI	TCCRAC	2 cut(s)	87, 263
MroNI	GCCGGC	1 cut(s)	514
MseI	TTAA	2 cut(s)	396, 546
MspA1I	CMGCKG	2 cut(s)	231, 322
MspI	CCGG	2 cut(s)	476, 515
MspR9I	CCNGG	3 cut(s)	14, 277, 566
MvaI	CCWGG	3 cut(s)	14, 277, 566
MvnI	CGCG	3 cut(s)	198, 231, 322
MwoI	GCNNNNNNNGC	4 cut(s)	48, 204, 331, 470
NaeI	GCCGGC	1 cut(s)	516
NcoI	CCATGG	1 cut(s)	552
NdeII	GATC	2 cut(s)	78, 96
NgoMIV	GCCGGC	1 cut(s)	514
NlaIII	CATG	1 cut(s)	556
NlaIV	GGNNCC	3 cut(s)	74, 194, 318
NmeAIII	GCCGAG	1 cut(s)	490
NmuCI	GTSAC	2 cut(s)	352, 413
NotI	GCGGCCGC	1 cut(s)	43
PceI	AGGCCT	1 cut(s)	564
PcsI	WCGNNNNNNNCGW	2 cut(s)	177, 254
PdiI	GCCGGC	1 cut(s)	516
PfeI	GAWTC	1 cut(s)	254
PflFI	GACNNNGTC	1 cut(s)	244
PkrI	GCNGC	7 cut(s)	44, 47, 50, 230, 321, 324, 361
Psp124BI	GAGCTC	1 cut(s)	31
Psp6I	CCWGG	3 cut(s)	12, 275, 564
PspGI	CCWGG	3 cut(s)	12, 275, 564
PspN4I	GGNNCC	3 cut(s)	74, 194, 318
PspPI	GGNCC	5 cut(s)	222, 273, 324, 556, 568
PsyI	GACNNNGTC	1 cut(s)	244
RsaI	GTAC	3 cut(s)	125, 194, 510
RsaNI	GTAC	3 cut(s)	124, 193, 509
SacI	GAGCTC	1 cut(s)	31
SacII	CCGCGG	2 cut(s)	232, 323
SaqAI	TTAA	2 cut(s)	396, 546
SatI	GCNGC	7 cut(s)	43, 46, 49, 229, 320, 323, 360
Sau3AI	GATC	2 cut(s)	78, 96
Sau96I	GGNCC	5 cut(s)	222, 273, 324, 556, 568
ScrFI	CCNGG	3 cut(s)	14, 277, 566
SduI	GDGCHC	2 cut(s)	31, 584
SetI	ASST	4 cut(s)	15, 31, 227, 245
Sfr303I	CCGCGG	2 cut(s)	232, 323
SgrBI	CCGCGG	2 cut(s)	232, 323
SinI	GGWCC	1 cut(s)	222
Sse9I	AATT	1 cut(s)	337
SseBI	AGGCCT	1 cut(s)	564
SsiI	CCGC	8 cut(s)	42, 46, 49, 196, 229, 231, 320, 322
SspMI	CTAG	2 cut(s)	188, 560
SstI	GAGCTC	1 cut(s)	31
StuI	AGGCCT	1 cut(s)	564
StyD4I	CCNGG	3 cut(s)	12, 275, 564
StyI	CCWWGG	3 cut(s)	187, 552, 559
TaaI	ACNGT	3 cut(s)	109, 292, 352
TaiI	ACGT	1 cut(s)	245
TaqI	TCGA	3 cut(s)	171, 248, 257
TasI	AATT	1 cut(s)	337
TauI	GCSGC	6 cut(s)	45, 48, 51, 231, 322, 325
TfiI	GAWTC	1 cut(s)	254
Tru1I	TTAA	2 cut(s)	396, 546
Tru9I	TTAA	2 cut(s)	396, 546
TseFI	GTSAC	2 cut(s)	352, 413
TseI	GCWGC	1 cut(s)	359
Tsp45I	GTSAC	2 cut(s)	352, 413
TspDTI	ATGAA	2 cut(s)	157, 267
TspGWI	ACGGA	1 cut(s)	514
Tth111I	GACNNNGTC	1 cut(s)	244
VpaK11BI	GGWCC	1 cut(s)	222
XmaJI	CCTAGG	2 cut(s)	187, 559
XspI	CTAG	2 cut(s)	188, 560
ZraI	GACGTC	1 cut(s)	243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.