Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Forward (+)

15317855 .. 15324792

Gene Structure 6938 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1174200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2820 bp

ATGAAGATTTATGAAGGGGTTGAAGAAAATGAGGGCAAGAAGGAGGTGGTTTTTGAAGTCGGTGATCCCCCAATTGAGGAAATCCATGATTTTTCAGAGAGGGAAGGGGCAATTGAGGAGAAAAATGTGAATTTTATTGAAGAGAAAGTGGAACCTGTGAAGAAAAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATAATAAGGACCCGTCATTGAGTGCTATGGCTGGATGGAAGGCCGTGAGGACTGGAGGGGATGGGAATGTTGGTGGCAGCGTTTTGGAGGTCAATAGTGGCTCAAATAACGAAGAGCTTTTGCAGTGTGATCTGGAAGAACATGGTTCACTGCCTTTTTACATGATTGATGCTTATGAAGAGTTCTATGGTGCTAATATGGGCAAGCTTTATCTGTTTGGGAAGGCCAAAGCAGGAAGTTCATACCAGAGTTGTTGTGTGGTTGTAGAGAACATGCAGAGATGTGTCTACGCAATTCCAAACAGTTCTGTATTTCACAGTGATGAAATCATGAAGCTTGAAAAGGATGCTGAAGAGTCGAGGATATCATCCAGAGAATTCCGTACAAAGCTGCATGACATGGCTTTGGGTTTAAAGAGTGACATAGCAAAGAAGTTGTTGGATCAAAATGTATCAGCTTTTAGCATGGCACTGGTTAAGAGAAACTATGCATTTGAGCGATCTGACATACCTGCCGGAGAGAATTATGTACTTAAGATCAATTATCCATTCAAGGATCCCCGACTTCCAGCAGATCTCAAAGGAGAAACATTCCATGCTCTTCTAGGAACACATAGCAGTGCGTTGGAGCTTTTTCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAGGTTCTGAGTTTCGCACAGTTTCACAACGGGTAAGTTGGTGCAAATTTGAGGTGGTCGTTAAATCTGCAAAAGACATACAGGTCTCAAATTGCCCAAAGAAAACTGCAGAGGCTCCCCCAGTGGCCAAGATTGACACTCCCGTGTTGGCATCAGAGTGGACGAGACCTGGCATGCTGAGCCATTTTACTGTTGTCCGTAAACTTGATGGAGGCATATTTCCCATGGGCGTCACTAAGGAGGCTACAGAAAAGAATTCTAAAGCTGGATCAAATGTTTTAAGCATCGAAAGCACCGAAAGGGCTTTACTAAACCGTTTGATGATTGAATTACACAAGTTGGACAGTGATGTTCTTGTTGGACACAATATATCTGGATTTGACCTGGATGTCCTACTCCGTAGAGCTAAGGCTTGTAAGGTGCCAAATGCCATGTGGTCCAAAATAGGCCGTCTCAAGTGTTCTGTGATGCCTGACCTTGCAAAAGGAGGCACCATTTTTGGATCTGGAGCAAGTCAAGGGATCATGTCCTGCATTTCTGGTCGCCTTTTGTGTGATACATACTTGTGTTCCCGTGACTTGTTAAGGGAGGTTAGCTATTCTTTGACACAACTTGCAAAGACTCAGCTGAATAAAGACCGAAAGGAGATCTTGCCGCATGAAATTCCTAGAATGTTCCAGAAATCAGAATTTCTAATGGAACTGATTGAATATGGTGAGACAGATGCATGGCTGTCTATGGAACTCATGTTTCATTTGAGTGTTCTTCCCCTCACCCGCCAGCTGACAAAGATAAGCGGTAATCTGTGGGGGAAAACCCTACAAGGTGCTAGGGCACAAAGAGTAGAATATCTTTTGCTGCATGCATTCCATGCAAAAAAATATATTGTTCCAGACAAGATGCATCTGAAGGAAACAGATTCGGTAAAGCGTAAAACAGATAATGGTGTTGATGATAAAAATGTTGATGAGTTGGATGTAAATTTTGATAATGATGCAAAGAAGGATCATGGGAAAGGCAAGAAAGGTCCTGCCTATGCAGGTGGATTGGTATTGGAGCCAAAAAGAGGGTTATATGACAAGTATATATTACTTCTCGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATCTGTTTCACAACAGTGGAAAGATCTTCAGAAGGATTGGCTCCTCGTTTACCATATTGTAAAAAAATTGGGCTCCTACCTGAGTTGTTAAAAGATCTAGTTGAAAGGAGGAGAAATGTAAAGAAATGGATGAAGACTGCATCTGGTCTCAAGATCCAGCAACTTGACATTCAACAGCAAGCTCTAAAGCTGACGGCACATAGTATGTATGGATGCTTGGGGTTTCCGAATTCGAGATTTTATGCAAAGCCACTGGCAGAGCTTATAACACTGCAAGGGAGAGAGATTCTGCAAAGTACTGTTGATCTTGGTCAGAACAATTTGAACCTAGAGGTGATCTATGGTGATACTGATTCAATGATGATTTATAGTGGACTGGATGATATTGCAAAAGCAAAAGCAATCGCACGGAAAGTCATTCAAGAGGTTAACAAGAAGTATAGGTGTTTGGAAATTGATCTTGACGGGTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCAGCTGTGAAGGTGCAATTCAAGAATGAGATGCCCTATGAGGTTATTGAGCGTAAGGGTCTTGATATGGTTTGCCAGGACTGGAGCTTACTTTCAAAGGAAATGGGAGATTTCTGCCTAAGTCAAATTTTATCAGGAGGGTCATGTGAGGATGTCGTTGAATCAATTCATGACGCTCTGATGAAGAAATCGAAGAGGCACCTACTTTTCCAGCCTTGTCAGCACTTGTCACACGCACACTCAGCTTTGCGGAAATTATGGGCATTCTGTCGAAGATTTCTGGCGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

940

Amino Acids

105.9

Weight (kDa)

8.59

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_B_exo1	PF03104	374 - 497	1.1e-15	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	563 - 907	6.1e-91	DNA polymerase family B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2288
AarI	CACCTGC	1 cut(s)	1913
AasI	GACNNNNNNGTC	2 cut(s)	965, 1271
Acc36I	ACCTGC	2 cut(s)	724, 1913
AccB1I	GGYRCC	3 cut(s)	1303, 1373, 2727
AccI	GTMKAC	1 cut(s)	492
AciI	CCGC	4 cut(s)	1538, 1660, 1680, 2779
AclWI	GGATC	9 cut(s)	59, 654, 755, 768, 1159, 1393, 1412, 1896, 2170
AcoI	YGGCCR	1 cut(s)	1008
AcsI	RAATTY	9 cut(s)	130, 581, 929, 1138, 1545, 1571, 1864, 2251, 2655
AcuI	CTGAAG	3 cut(s)	576, 1811, 2034
AcyI	GRCGYC	1 cut(s)	1113
AfaI	GTAC	4 cut(s)	589, 735, 2320, 2495
AfiI	CCNNNNNNNGG	2 cut(s)	76, 1949
AflII	CTTAAG	1 cut(s)	737
AjnI	CCWGG	3 cut(s)	1051, 1266, 2604
AleI	CACNNNNGTG	2 cut(s)	1025, 2036
Alw26I	GTCTC	5 cut(s)	973, 1042, 1340, 1595, 2174
AlwI	GGATC	9 cut(s)	59, 654, 755, 768, 1159, 1393, 1412, 1896, 2170
AlwNI	CAGNNNCTG	1 cut(s)	890
AoxI	GGCC	4 cut(s)	246, 429, 1008, 1330
ApeKI	GCWGC	5 cut(s)	282, 595, 1741, 2506, 2531
ApoI	RAATTY	9 cut(s)	130, 581, 929, 1138, 1545, 1571, 1864, 2251, 2655
Asp700I	GAANNNNTTC	1 cut(s)	2694
AspS9I	GGNCC	4 cut(s)	214, 863, 1320, 1910
AsuHPI	GGTGA	5 cut(s)	74, 1610, 1648, 2368, 2378
AvaII	GGWCC	4 cut(s)	214, 863, 1320, 1910
BaeGI	GKGCMC	1 cut(s)	1720
BalI	TGGCCA	1 cut(s)	1010
BamHI	GGATCC	1 cut(s)	760
BanI	GGYRCC	3 cut(s)	1303, 1373, 2727
BanII	GRGCYC	1 cut(s)	2097
BbsI	GAAGAC	1 cut(s)	2162
BbvI	GCAGC	5 cut(s)	294, 582, 1728, 2493, 2543
BccI	CCATC	3 cut(s)	234, 260, 1085
BceAI	ACGGC	3 cut(s)	233, 1317, 2232
BciT130I	CCWGG	3 cut(s)	1053, 1268, 2606
BcoDI	GTCTC	5 cut(s)	973, 1042, 1340, 1595, 2174
BfaI	CTAG	5 cut(s)	809, 1551, 1713, 2120, 2351
BfmI	CTRYAG	2 cut(s)	990, 1128
BfrI	CTTAAG	1 cut(s)	737
BfuAI	ACCTGC	2 cut(s)	724, 1913
BglII	AGATCT	4 cut(s)	778, 1530, 2045, 2116
BisI	GCNGC	6 cut(s)	283, 596, 1538, 1742, 2507, 2532
BlpI	GCTNAGC	1 cut(s)	1061
BlsI	GCNGC	6 cut(s)	284, 597, 1539, 1743, 2508, 2533
BmcAI	AGTACT	1 cut(s)	2320
Bme1390I	CCNGG	3 cut(s)	1053, 1268, 2606
Bme18I	GGWCC	4 cut(s)	214, 863, 1320, 1910
BmgT120I	GGNCC	4 cut(s)	214, 863, 1320, 1910
BmrFI	CCNGG	3 cut(s)	1053, 1268, 2606
BmrI	ACTGGG	1 cut(s)	998
BmuI	ACTGGG	1 cut(s)	998
BpiI	GAAGAC	1 cut(s)	2162
BpmI	CTGGAG	3 cut(s)	279, 1410, 2632
Bpu10I	CCTNAGC	1 cut(s)	1290
Bpu1102I	GCTNAGC	1 cut(s)	1061
BpuEI	CTTGAG	3 cut(s)	1322, 2156, 2498
BsaHI	GRCGYC	1 cut(s)	1113
BsaI	GGTCTC	3 cut(s)	973, 1042, 2174
BsaJI	CCNNGG	1 cut(s)	1107
Bsc4I	CCNNNNNNNGG	2 cut(s)	76, 1949
Bse1I	ACTGG	6 cut(s)	262, 681, 1004, 2280, 2403, 2615
BseBI	CCWGG	3 cut(s)	1053, 1268, 2606
BseDI	CCNNGG	1 cut(s)	1107
BseLI	CCNNNNNNNGG	2 cut(s)	76, 1949
BseMII	CTCAG	5 cut(s)	882, 1052, 1520, 2094, 2784
BseNI	ACTGG	6 cut(s)	262, 681, 1004, 2280, 2403, 2615
BseRI	GAGGAG	3 cut(s)	131, 2055, 2146
BseSI	GKGCMC	1 cut(s)	1720
BseXI	GCAGC	5 cut(s)	294, 582, 1728, 2493, 2543
BshFI	GGCC	4 cut(s)	248, 431, 1010, 1332
BshNI	GGYRCC	3 cut(s)	1303, 1373, 2727
BsiSI	CCGG	1 cut(s)	720
BslI	CCNNNNNNNGG	2 cut(s)	76, 1949
BsmAI	GTCTC	5 cut(s)	973, 1042, 1340, 1595, 2174
BsmBI	CGTCTC	1 cut(s)	1340
BsmI	GAATGC	4 cut(s)	193, 1748, 2508, 2792
BsnI	GGCC	4 cut(s)	248, 431, 1010, 1332
Bso31I	GGTCTC	3 cut(s)	973, 1042, 2174
Bsp1286I	GDGCHC	2 cut(s)	1720, 2097
Bsp1407I	TGTACA	1 cut(s)	2493
Bsp1720I	GCTNAGC	1 cut(s)	1061
Bsp19I	CCATGG	1 cut(s)	1107
BspACI	CCGC	4 cut(s)	1538, 1660, 1680, 2779
BspANI	GGCC	4 cut(s)	248, 431, 1010, 1332
BspCNI	CTCAG	5 cut(s)	883, 1053, 1519, 2095, 2783
BspHI	TCATGA	2 cut(s)	534, 2698
BspMAI	CTGCAG	1 cut(s)	994
BspMI	ACCTGC	2 cut(s)	724, 1913
BspPI	GGATC	9 cut(s)	59, 654, 755, 768, 1159, 1393, 1412, 1896, 2170
BspQI	GCTCTTC	2 cut(s)	312, 810
BspT107I	GGYRCC	3 cut(s)	1303, 1373, 2727
BspTI	CTTAAG	1 cut(s)	737
BspTNI	GGTCTC	3 cut(s)	973, 1042, 2174
BsrGI	TGTACA	1 cut(s)	2493
BsrI	ACTGG	6 cut(s)	262, 681, 1004, 2280, 2403, 2615
BssECI	CCNNGG	1 cut(s)	1107
BssNI	GRCGYC	1 cut(s)	1113
BssT1I	CCWWGG	1 cut(s)	1107
Bst2UI	CCWGG	3 cut(s)	1053, 1268, 2606
Bst4CI	ACNGT	8 cut(s)	509, 524, 904, 1075, 1199, 1229, 2038, 2323
Bst6I	CTCTTC	7 cut(s)	135, 165, 312, 378, 552, 810, 2717
BstACI	GRCGYC	1 cut(s)	1113
BstAFI	CTTAAG	1 cut(s)	737
BstAPI	GCANNNNNTGC	1 cut(s)	1754
BstAUI	TGTACA	1 cut(s)	2493
BstC8I	GCNNGC	5 cut(s)	410, 1058, 1664, 1746, 2202
BstDEI	CTNAG	8 cut(s)	891, 1061, 1119, 1290, 1506, 2103, 2648, 2770
BstDSI	CCRYGG	1 cut(s)	1107
BstMAI	GTCTC	5 cut(s)	973, 1042, 1340, 1595, 2174
BstMWI	GCNNNNNNNGC	5 cut(s)	1062, 1173, 1754, 2749, 2771
BstNI	CCWGG	3 cut(s)	1053, 1268, 2606
BstNSI	RCATGY	3 cut(s)	481, 1060, 1748
BstSCI	CCNGG	3 cut(s)	1051, 1266, 2604
BstSFI	CTRYAG	2 cut(s)	990, 1128
BstSLI	GKGCMC	1 cut(s)	1720
BstV1I	GCAGC	5 cut(s)	294, 582, 1728, 2493, 2543
BstV2I	GAAGAC	1 cut(s)	2162
BstX2I	RGATCY	7 cut(s)	760, 778, 1385, 1530, 2045, 2116, 2175
BstYI	RGATCY	7 cut(s)	760, 778, 1385, 1530, 2045, 2116, 2175
BsuRI	GGCC	4 cut(s)	248, 431, 1010, 1332
BtgI	CCRYGG	1 cut(s)	1107
BtsI	GCAGTG	4 cut(s)	335, 353, 829, 2291
BveI	ACCTGC	2 cut(s)	724, 1913
Cac8I	GCNNGC	5 cut(s)	410, 1058, 1664, 1746, 2202
CaiI	CAGNNNCTG	1 cut(s)	890
CciI	TCATGA	2 cut(s)	534, 2698
Cfr13I	GGNCC	4 cut(s)	214, 863, 1320, 1910
CseI	GACGC	2 cut(s)	1102, 2711
Csp6I	GTAC	4 cut(s)	588, 734, 2319, 2494
CviQI	GTAC	4 cut(s)	588, 734, 2319, 2494
DdeI	CTNAG	8 cut(s)	891, 1061, 1119, 1290, 1506, 2103, 2648, 2770
DraI	TTTAAA	1 cut(s)	618
DrdI	GACNNNNNNGTC	2 cut(s)	965, 1271
DseDI	GACNNNNNNGTC	2 cut(s)	965, 1271
EaeI	YGGCCR	1 cut(s)	1008
Eam1104I	CTCTTC	7 cut(s)	135, 165, 312, 378, 552, 810, 2717
EarI	CTCTTC	7 cut(s)	135, 165, 312, 378, 552, 810, 2717
Eco130I	CCWWGG	1 cut(s)	1107
Eco24I	GRGCYC	1 cut(s)	2097
Eco31I	GGTCTC	3 cut(s)	973, 1042, 2174
Eco32I	GATATC	1 cut(s)	570
Eco47I	GGWCC	4 cut(s)	214, 863, 1320, 1910
Eco57I	CTGAAG	3 cut(s)	576, 1811, 2034
EcoO109I	RGGNCCY	3 cut(s)	214, 863, 1910
EcoRI	GAATTC	3 cut(s)	581, 1138, 2251
EcoRII	CCWGG	3 cut(s)	1051, 1266, 2604
EcoRV	GATATC	1 cut(s)	570
EcoT14I	CCWWGG	1 cut(s)	1107
EcoT22I	ATGCAT	4 cut(s)	697, 1612, 1750, 1788
EcoT38I	GRGCYC	1 cut(s)	2097
ErhI	CCWWGG	1 cut(s)	1107
Esp3I	CGTCTC	1 cut(s)	1340
FalI	AAGNNNNNCTT	2 cut(s)	1517, 1549
FauI	CCCGC	1 cut(s)	1667
FblI	GTMKAC	1 cut(s)	492
Fnu4HI	GCNGC	6 cut(s)	283, 596, 1538, 1742, 2507, 2532
FriOI	GRGCYC	1 cut(s)	2097
Fsp4HI	GCNGC	6 cut(s)	283, 596, 1538, 1742, 2507, 2532
FspBI	CTAG	5 cut(s)	809, 1551, 1713, 2120, 2351
GluI	GCNGC	6 cut(s)	283, 596, 1538, 1742, 2507, 2532
GsuI	CTGGAG	3 cut(s)	279, 1410, 2632
HaeIII	GGCC	4 cut(s)	248, 431, 1010, 1332
HapII	CCGG	1 cut(s)	720
HgaI	GACGC	2 cut(s)	1102, 2711
Hin1I	GRCGYC	1 cut(s)	1113
HincII	GTYRAC	1 cut(s)	2452
HindII	GTYRAC	1 cut(s)	2452
HindIII	AAGCTT	2 cut(s)	410, 539
HinfI	GANTC	6 cut(s)	560, 1504, 1802, 2308, 2375, 2690
HpaI	GTTAAC	1 cut(s)	2452
HpaII	CCGG	1 cut(s)	720
HphI	GGTGA	5 cut(s)	74, 1610, 1648, 2368, 2378
Hpy166II	GTNNAC	7 cut(s)	353, 493, 1044, 1085, 2072, 2396, 2452
Hpy8I	GTNNAC	7 cut(s)	353, 493, 1044, 1085, 2072, 2396, 2452
HpyCH4III	ACNGT	8 cut(s)	509, 524, 904, 1075, 1199, 1229, 2038, 2323
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1062, 1173, 1754, 2749, 2771
HpyF3I	CTNAG	8 cut(s)	891, 1061, 1119, 1290, 1506, 2103, 2648, 2770
Hsp92I	GRCGYC	1 cut(s)	1113
KspAI	GTTAAC	1 cut(s)	2452
LguI	GCTCTTC	2 cut(s)	312, 810
LmnI	GCTCC	7 cut(s)	832, 1003, 1391, 1939, 2068, 2100, 2613
Lsp1109I	GCAGC	5 cut(s)	294, 582, 1728, 2493, 2543
MaeI	CTAG	5 cut(s)	809, 1551, 1713, 2120, 2351
MaeIII	GTNAC	4 cut(s)	623, 1114, 1457, 2757
MfeI	CAATTG	2 cut(s)	72, 111
MflI	RGATCY	7 cut(s)	760, 778, 1385, 1530, 2045, 2116, 2175
MhlI	GDGCHC	2 cut(s)	1720, 2097
MlsI	TGGCCA	1 cut(s)	1010
MluNI	TGGCCA	1 cut(s)	1010
MlyI	GAGTC	2 cut(s)	569, 1498
MmeI	TCCRAC	5 cut(s)	624, 810, 1203, 1222, 1836
Mox20I	TGGCCA	1 cut(s)	1010
Mph1103I	ATGCAT	4 cut(s)	697, 1612, 1750, 1788
MroXI	GAANNNNTTC	1 cut(s)	2694
MscI	TGGCCA	1 cut(s)	1010
MslI	CAYNNNNRTG	7 cut(s)	525, 822, 1025, 1039, 1447, 1641, 2036
Msp20I	TGGCCA	1 cut(s)	1010
MspA1I	CMGCKG	3 cut(s)	1510, 1666, 2534
MspCI	CTTAAG	1 cut(s)	737
MspI	CCGG	1 cut(s)	720
MspR9I	CCNGG	3 cut(s)	1053, 1268, 2606
MunI	CAATTG	2 cut(s)	72, 111
Mva1269I	GAATGC	4 cut(s)	193, 1748, 2508, 2792
MvaI	CCWGG	3 cut(s)	1053, 1268, 2606
MwoI	GCNNNNNNNGC	5 cut(s)	1062, 1173, 1754, 2749, 2771
NcoI	CCATGG	1 cut(s)	1107
NmuCI	GTSAC	4 cut(s)	623, 1114, 1457, 2757
NsiI	ATGCAT	4 cut(s)	697, 1612, 1750, 1788
NspI	RCATGY	3 cut(s)	481, 1060, 1748
OliI	CACNNNNGTG	2 cut(s)	1025, 2036
PaeI	GCATGC	2 cut(s)	1060, 1748
PagI	TCATGA	2 cut(s)	534, 2698
PaqCI	CACCTGC	1 cut(s)	1913
PciSI	GCTCTTC	2 cut(s)	312, 810
PcsI	WCGNNNNNNNCGW	1 cut(s)	1176
PctI	GAATGC	4 cut(s)	193, 1748, 2508, 2792
PdmI	GAANNNNTTC	1 cut(s)	2694
PfeI	GAWTC	4 cut(s)	1802, 2308, 2375, 2690
PkrI	GCNGC	6 cut(s)	284, 597, 1539, 1743, 2508, 2533
PleI	GAGTC	2 cut(s)	568, 1498
PpsI	GAGTC	2 cut(s)	568, 1498
PpuMI	RGGWCCY	3 cut(s)	214, 863, 1910
PsiI	TTATAA	1 cut(s)	2288
Psp5II	RGGWCCY	3 cut(s)	214, 863, 1910
Psp6I	CCWGG	3 cut(s)	1051, 1266, 2604
PspGI	CCWGG	3 cut(s)	1051, 1266, 2604
PspPI	GGNCC	4 cut(s)	214, 863, 1320, 1910
PspPPI	RGGWCCY	3 cut(s)	214, 863, 1910
PstI	CTGCAG	1 cut(s)	994
PstNI	CAGNNNCTG	1 cut(s)	890
PsuI	RGATCY	7 cut(s)	760, 778, 1385, 1530, 2045, 2116, 2175
PvuII	CAGCTG	3 cut(s)	1510, 1666, 2534
RsaI	GTAC	4 cut(s)	589, 735, 2320, 2495
RsaNI	GTAC	4 cut(s)	588, 734, 2319, 2494
RseI	CAYNNNNRTG	7 cut(s)	525, 822, 1025, 1039, 1447, 1641, 2036
SapI	GCTCTTC	2 cut(s)	312, 810
SatI	GCNGC	6 cut(s)	283, 596, 1538, 1742, 2507, 2532
Sau96I	GGNCC	4 cut(s)	214, 863, 1320, 1910
ScaI	AGTACT	1 cut(s)	2320
SchI	GAGTC	2 cut(s)	569, 1498
ScrFI	CCNGG	3 cut(s)	1053, 1268, 2606
SduI	GDGCHC	2 cut(s)	1720, 2097
SfcI	CTRYAG	2 cut(s)	990, 1128
SinI	GGWCC	4 cut(s)	214, 863, 1320, 1910
SmiMI	CAYNNNNRTG	7 cut(s)	525, 822, 1025, 1039, 1447, 1641, 2036
SmlI	CTYRAG	4 cut(s)	737, 1337, 2171, 2513
SmoI	CTYRAG	4 cut(s)	737, 1337, 2171, 2513
SphI	GCATGC	2 cut(s)	1060, 1748
SsiI	CCGC	4 cut(s)	1538, 1660, 1680, 2779
SspMI	CTAG	5 cut(s)	809, 1551, 1713, 2120, 2351
StyD4I	CCNGG	3 cut(s)	1051, 1266, 2604
StyI	CCWWGG	1 cut(s)	1107
TaaI	ACNGT	8 cut(s)	509, 524, 904, 1075, 1199, 1229, 2038, 2323
TaqI	TCGA	6 cut(s)	563, 1170, 1980, 2255, 2720, 2800
TaqII	GACCGA	1 cut(s)	1536
TatI	WGTACW	3 cut(s)	733, 2318, 2493
TauI	GCSGC	1 cut(s)	1540
TfiI	GAWTC	4 cut(s)	1802, 2308, 2375, 2690
TseFI	GTSAC	4 cut(s)	623, 1114, 1457, 2757
TseI	GCWGC	5 cut(s)	282, 595, 1741, 2506, 2531
Tsp45I	GTSAC	4 cut(s)	623, 1114, 1457, 2757
TspGWI	ACGGA	4 cut(s)	575, 1070, 1271, 2446
Vha464I	CTTAAG	1 cut(s)	737
VpaK11BI	GGWCC	4 cut(s)	214, 863, 1320, 1910
XapI	RAATTY	9 cut(s)	130, 581, 929, 1138, 1545, 1571, 1864, 2251, 2655
XceI	RCATGY	3 cut(s)	481, 1060, 1748
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1314
XmiI	GTMKAC	1 cut(s)	492
XmnI	GAANNNNTTC	1 cut(s)	2694
XspI	CTAG	5 cut(s)	809, 1551, 1713, 2120, 2351
ZrmI	AGTACT	1 cut(s)	2320
Zsp2I	ATGCAT	4 cut(s)	697, 1612, 1750, 1788

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.