Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Reverse (-)

29339128 .. 29348868

Gene Structure 9741 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ49002

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4290 bp

ATGAGGCCCAGGAGGAGGAAGAAGGTGGAGAAGAAGGAGAAGGAGAAGGAGCCGCGGCCCAAAAAGCCCAATTCGTCGCTTACGGCGGCGGCGGCGATGATGGGGAAGCAGAGGCTTTCGTCGATGTTCACGTCGTCGGTGTTTAATAAAAGTAGGGATGGTGACAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCCGAGTTTGCGCCGGACGAGGCTGATAGAGAGAAACGGAGGAGGGCACAGCCGGCGAGGAGTTTTGTTCCGATTGCCGGTGTTAAGAGTGAGAGAGTGAGTAATGGGGGTGTTGAGTTATCAAGGAGGCTTGAATTGGATAGAATTGTTGCAAATGGTAGTGGTTCTGTGCATTGTAAGGAAATGGATAAAGATTCTGAGGGCAATAAAGAGTTGGATTTCGGAAATTGTCAGCCCCAAATTGAGCAATTCCATGATTTTTTGGAGAATAGTGAGGTGCCCGAGGAGAAGAAGACTGAGGATTCTGCTGAGGTGCAGGCGGAGCCTGTGGTGAAGAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATGAGGATCCGGCATTAAGTGCTATGGCAGGATGGAAGGCCGTGATGACTGGTGGGGCCGGGGTTACTGGTGGCAGCGGCAATGAGGAGGTCAAAAGTGACTCAATTAGTGAAGGGAGTTCGGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAATATGGGCACTGTTTATCTATTTGGGAAGGTTAAAGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTAAAGAACATGCAAAGATGTGTCTATGCAATTCCGGACAGTCCTTTATTTCAAACTGACGAAATGATGAAGCTTGAAAAGGATGCCGAGGACTCAAGGATTTCACCCACAGATTTTCGTAAGAAGCTGCATGATGCGGCTTCAGAATTAAAGAATGAATTAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGGCACCTGTCAAGAGGAACTATGCTTTTGAGCGATCTGACATACCTGCAGGGGAGAATTATGTACTGAAGATCAATTATCCATTCCGGGATCCACCACTATCAGCAGATCTAAAAGGAGAAACTTTTTGTGCTCTTCTAGGAACTCATAGCAGCGCGTTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAAATTTTTCCATCTGCCCAGCTCCACAACGGGTAAGCTGGTGCAAATTTGAGGTGATCGTGAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACAAAGAAAACTGTAGATATTCCTCAAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTATCAATGAGAAGCAGAATGTCAACGAAATTGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCTGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGTGGCATATTTCCTATGGGGTTTACTAAAGTGGCTGCAGATAAGAATTCTAAAGCCGGATCAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTAAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTTGTTGGACATAATATATCTGGATTTGATCTGGATGTTCTTCTCCACAGAGCTCAGGTTTGCAGAGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATCATGTCCTGCATTGCTGGTCGGCTGTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAAGGAGGTCAGCTATTCCTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCGCATGATATTCCTAGAATGTTACAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCCTGGTTGTCTATGGGACTCATGTTTCATTTGAGTGCTCTTCCCCTCACACGTCAGCTGACTAATATAAGTGGCAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTAGAATATCTCTTACTGCATGCTTTTCATGCAAAAAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGTGTTGAGGAAAAAGCTGCTGATGAGTTGGATGTAAATGATGTAAATGTTGATAATGATGCACAGAGTGGTCATGGAAAAGGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTATTGGAGCCAAAAAAAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATATGCTTCACAACTGTTGAAAGAAATTCAGAAGGATTGGTTTCTCATTTACCTTCTAGTCAAAGACCTGGACTTTTACCTGAGTTGTTAAAAAATTTAGTTGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAACTTGACATTCAACAGCAAGCTCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAACTCAAGATTTTACGCAAAGCCACTGGCAGAGCTTATAACACTACGGGGCAGGGAGATCCTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTAATCTATGGTGATACGGACTCAATAATGATTTATAGTGGGCTGGATGATAGTGTGAAAGCAAAAGCAATTGCTGTGAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGACGGCTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCGGCTGTGAAGGAGCTAACGAAAAACGAGAAAAAATACGAGGTTATCGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGTTTACTTTCAAAGGAACTGGGAGATTTCTGCTTAAGTCAAATTTTGTCAGGAGGGTCATCTGAAGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTGCCCTCGAGAAATATATCATCACCAAGACATTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAGTGGTTACTCTACTGGTTGCTCTGTTGGAGATACAGTCCCTTATATTATTTGCTGTGAGCAGGGCACAAGTTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAGATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCTGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACCAGCCCCCAACGCTTGGCTGATTGTTTAGGGATTGACTCATCCAAGTTCAAATCGAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTGTTGACGATGAAGAGAGATATCGGGATTGTGAGTCTTTGATCTTAGCATGTCCTAGCTGCTCTGGTACTTTTGATTGTCCAGCCATCTTCAACTACATTGCCAAGTCAATCAATGAAAAGCCAACAAGACCACAAGGAGAAGAATCTTCTAGCGATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCCAATCAGGTCAAAAGGCAAGCAGAGGGTTTTGTTTCAATGTACTACAGGGGCATAATGATGTGTGATGATGAAACTTGCAAATACACCACACGAAGTCTCAACCTCCGGCTTGTTGGTGATGCTGAGAGAGGAACAGTCTGTCCAAACTATCCTCGCTGCAATGGGCATCTTGTAAGAAAGTACACGGAAGCTGATTTGTACAAACAGCTCTCATTTTTCTGCCATGTCTTGGATACAGTACGCTGCGTAGAAAAGATGGAACCTAAGACTAGACTGCCGGTAGAGTTAGAGTTGGCCAAAATTCGCCCCATTGTGACTTTGGCTGCCTCAACAGTTGAAAGGATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTGGAAGATCTTTTTGTTACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1429

Amino Acids

159.7

Weight (kDa)

8.37

Isoelectric Point (pI)

43.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_B_exo1	PF03104	400 - 654	2e-26	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	720 - 1182	2.6e-125	DNA polymerase family B
zf-DNA_Pol	PF08996	1220 - 1426	6.4e-43	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2771
Acc36I	ACCTGC	1 cut(s)	1105
Acc65I	GGTACC	1 cut(s)	3551
AccB1I	GGYRCC	3 cut(s)	487, 1054, 3551
AccB7I	CCANNNNNTGG	2 cut(s)	3568, 4117
AccII	CGCG	2 cut(s)	55, 1208
AccIII	TCCGGA	1 cut(s)	883
AclWI	GGATC	9 cut(s)	587, 600, 1035, 1136, 1149, 1630, 1883, 2785, 2817
AcoI	YGGCCR	1 cut(s)	4182
AcsI	RAATTY	8 cut(s)	1267, 1310, 1609, 2530, 2599, 3138, 3610, 4188
AcuI	CTGAAG	5 cut(s)	975, 1139, 2285, 2711, 3180
AdeI	CACNNNGTG	1 cut(s)	2369
AfiI	CCNNNNNNNGG	9 cut(s)	15, 287, 785, 2432, 3079, 3568, 3851, 4117, 4254
AflII	CTTAAG	1 cut(s)	3130
AflIII	ACRYGT	2 cut(s)	2129, 3304
AjiI	CACGTC	2 cut(s)	132, 2132
AjnI	CCWGG	5 cut(s)	8, 1522, 1870, 2081, 2572
AleI	CACNNNNGTG	1 cut(s)	1496
AloI	GAACNNNNNNTCC	2 cut(s)	4012, 4044
Alw21I	GWGCWC	3 cut(s)	1186, 1762, 2119
Alw26I	GTCTC	6 cut(s)	1354, 1456, 2066, 2657, 3876, 3989
AlwI	GGATC	9 cut(s)	587, 600, 1035, 1136, 1149, 1630, 1883, 2785, 2817
Ama87I	CYCGRG	2 cut(s)	491, 3233
Aor13HI	TCCGGA	1 cut(s)	883
ApoI	RAATTY	8 cut(s)	1267, 1310, 1609, 2530, 2599, 3138, 3610, 4188
Asp700I	GAANNNNTTC	3 cut(s)	3177, 3634, 4230
Asp718I	GGTACC	1 cut(s)	3551
AspLEI	GCGC	4 cut(s)	223, 1208, 3319, 3831
AsuC2I	CCSGG	3 cut(s)	646, 1139, 3421
AsuHPI	GGTGA	8 cut(s)	173, 553, 945, 1330, 2861, 3241, 3866, 4016
AvaI	CYCGRG	2 cut(s)	491, 3233
AvaII	GGWCC	5 cut(s)	776, 1244, 1791, 2393, 4237
BaeGI	GKGCMC	5 cut(s)	259, 492, 791, 2191, 3401
BalI	TGGCCA	1 cut(s)	4184
BamHI	GGATCC	2 cut(s)	592, 1141
BanI	GGYRCC	3 cut(s)	487, 1054, 3551
BanII	GRGCYC	1 cut(s)	1762
BbsI	GAAGAC	1 cut(s)	509
Bbv12I	GWGCWC	3 cut(s)	1186, 1762, 2119
BbvCI	CCTCAGC	1 cut(s)	519
BceAI	ACGGC	3 cut(s)	99, 611, 2986
BcgI	CGANNNNNNTGC	2 cut(s)	2060, 2094
BciT130I	CCWGG	5 cut(s)	10, 1524, 1872, 2083, 2574
BciVI	GTATCC	3 cut(s)	2263, 4114, 4251
BcnI	CCSGG	3 cut(s)	646, 1139, 3421
BcoDI	GTCTC	6 cut(s)	1354, 1456, 2066, 2657, 3876, 3989
BfaI	CTAG	7 cut(s)	1190, 2022, 2562, 3438, 3711, 3807, 4158
BfmI	CTRYAG	4 cut(s)	1098, 1371, 1599, 3931
BfrI	CTTAAG	1 cut(s)	3130
BfuAI	ACCTGC	1 cut(s)	1105
BfuI	GTATCC	3 cut(s)	2263, 4114, 4251
BglII	AGATCT	2 cut(s)	1159, 4270
BlpI	GCTNAGC	1 cut(s)	1532
BmcAI	AGTACT	1 cut(s)	2803
Bme1390I	CCNGG	8 cut(s)	10, 646, 1139, 1524, 1872, 2083, 2574, 3421
Bme18I	GGWCC	5 cut(s)	776, 1244, 1791, 2393, 4237
BmeT110I	CYCGRG	2 cut(s)	491, 3233
BmgBI	CACGTC	2 cut(s)	132, 2132
BmrFI	CCNGG	8 cut(s)	10, 646, 1139, 1524, 1872, 2083, 2574, 3421
BmrI	ACTGGG	1 cut(s)	3125
BmuI	ACTGGG	1 cut(s)	3125
BpiI	GAAGAC	1 cut(s)	509
BpmI	CTGGAG	2 cut(s)	1881, 3115
Bpu10I	CCTNAGC	3 cut(s)	519, 1761, 1827
Bpu1102I	GCTNAGC	1 cut(s)	1532
BpuEI	CTTGAG	7 cut(s)	928, 1368, 1654, 1793, 2639, 2722, 2981
BpuMI	CCSGG	3 cut(s)	646, 1139, 3421
BsaBI	GATNNNNATC	2 cut(s)	591, 714
BsaI	GGTCTC	2 cut(s)	1354, 2657
BsaJI	CCNNGG	7 cut(s)	8, 53, 492, 645, 936, 1871, 2176
BsaWI	WCCGGW	2 cut(s)	883, 1992
BsaXI	ACNNNNNCTCC	2 cut(s)	3354, 3384
Bsc4I	CCNNNNNNNGG	9 cut(s)	15, 287, 785, 2432, 3079, 3568, 3851, 4117, 4254
Bse118I	RCCGGY	3 cut(s)	262, 287, 4165
Bse1I	ACTGG	6 cut(s)	640, 658, 2763, 3098, 3120, 3352
Bse3DI	GCAATG	4 cut(s)	673, 1887, 3753, 4054
Bse8I	GATNNNNATC	2 cut(s)	591, 714
BseAI	TCCGGA	1 cut(s)	883
BseBI	CCWGG	5 cut(s)	10, 1524, 1872, 2083, 2574
BseDI	CCNNGG	7 cut(s)	8, 53, 492, 645, 936, 1871, 2176
BseJI	GATNNNNATC	2 cut(s)	591, 714
BseLI	CCNNNNNNNGG	9 cut(s)	15, 287, 785, 2432, 3079, 3568, 3851, 4117, 4254
BseMI	GCAATG	4 cut(s)	673, 1887, 3753, 4054
BseNI	ACTGG	6 cut(s)	640, 658, 2763, 3098, 3120, 3352
BseRI	GAGGAG	6 cut(s)	28, 265, 283, 509, 686, 2629
BseSI	GKGCMC	5 cut(s)	259, 492, 791, 2191, 3401
BseYI	CCCAGC	2 cut(s)	1282, 1981
BsgI	GTGCAG	2 cut(s)	545, 3841
Bsh1236I	CGCG	2 cut(s)	55, 1208
BshNI	GGYRCC	3 cut(s)	487, 1054, 3551
BsiHKAI	GWGCWC	3 cut(s)	1186, 1762, 2119
BsiHKCI	CYCGRG	2 cut(s)	491, 3233
BslFI	GGGAC	4 cut(s)	2109, 3356, 3487, 4250
BslI	CCNNNNNNNGG	9 cut(s)	15, 287, 785, 2432, 3079, 3568, 3851, 4117, 4254
BsmAI	GTCTC	6 cut(s)	1354, 1456, 2066, 2657, 3876, 3989
BsmFI	GGGAC	4 cut(s)	2109, 3356, 3487, 4250
BsmI	GAATGC	4 cut(s)	574, 1333, 2647, 2991
Bso31I	GGTCTC	2 cut(s)	1354, 2657
BsoBI	CYCGRG	2 cut(s)	491, 3233
Bsp1286I	GDGCHC	8 cut(s)	259, 492, 791, 1186, 1762, 2119, 2191, 3401
Bsp13I	TCCGGA	1 cut(s)	883
Bsp1407I	TGTACA	2 cut(s)	2976, 4086
Bsp1720I	GCTNAGC	1 cut(s)	1532
BspEI	TCCGGA	1 cut(s)	883
BspFNI	CGCG	2 cut(s)	55, 1208
BspMAI	CTGCAG	2 cut(s)	1102, 1603
BspMI	ACCTGC	1 cut(s)	1105
BspPI	GGATC	9 cut(s)	587, 600, 1035, 1136, 1149, 1630, 1883, 2785, 2817
BspQI	GCTCTTC	2 cut(s)	1191, 2124
BspT107I	GGYRCC	3 cut(s)	487, 1054, 3551
BspTI	CTTAAG	1 cut(s)	3130
BspTNI	GGTCTC	2 cut(s)	1354, 2657
BsrDI	GCAATG	4 cut(s)	673, 1887, 3753, 4054
BsrFI	RCCGGY	3 cut(s)	262, 287, 4165
BsrGI	TGTACA	2 cut(s)	2976, 4086
BsrI	ACTGG	6 cut(s)	640, 658, 2763, 3098, 3120, 3352
BssAI	RCCGGY	3 cut(s)	262, 287, 4165
BssECI	CCNNGG	7 cut(s)	8, 53, 492, 645, 936, 1871, 2176
BssT1I	CCWWGG	1 cut(s)	2176
Bst2UI	CCWGG	5 cut(s)	10, 1524, 1872, 2083, 2574
Bst6I	CTCTTC	6 cut(s)	546, 759, 1191, 1511, 2124, 3663
BstAFI	CTTAAG	1 cut(s)	3130
BstAPI	GCANNNNNTGC	1 cut(s)	605
BstAUI	TGTACA	2 cut(s)	2976, 4086
BstC8I	GCNNGC	6 cut(s)	264, 528, 1833, 2217, 2685, 3906
BstDSI	CCRYGG	1 cut(s)	53
BstFNI	CGCG	2 cut(s)	55, 1208
BstHHI	GCGC	4 cut(s)	223, 1208, 3319, 3831
BstMAI	GTCTC	6 cut(s)	1354, 1456, 2066, 2657, 3876, 3989
BstNI	CCWGG	5 cut(s)	10, 1524, 1872, 2083, 2574
BstNSI	RCATGY	6 cut(s)	751, 862, 1789, 2219, 3308, 3708
BstSCI	CCNGG	8 cut(s)	8, 644, 1137, 1522, 1870, 2081, 2572, 3419
BstSFI	CTRYAG	4 cut(s)	1098, 1371, 1599, 3931
BstSLI	GKGCMC	5 cut(s)	259, 492, 791, 2191, 3401
BstUI	CGCG	2 cut(s)	55, 1208
BstV2I	GAAGAC	1 cut(s)	509
BstX2I	RGATCY	7 cut(s)	592, 1027, 1141, 1159, 2790, 2809, 4270
BstYI	RGATCY	7 cut(s)	592, 1027, 1141, 1159, 2790, 2809, 4270
BsuI	GTATCC	3 cut(s)	2263, 4114, 4251
BtgI	CCRYGG	1 cut(s)	53
BtgZI	GCGATG	1 cut(s)	110
BtrI	CACGTC	2 cut(s)	132, 2132
BtsI	GCAGTG	2 cut(s)	734, 1654
BtsIMutI	CAGTG	4 cut(s)	734, 789, 1654, 2756
BveI	ACCTGC	1 cut(s)	1105
Cac8I	GCNNGC	6 cut(s)	264, 528, 1833, 2217, 2685, 3906
CfoI	GCGC	4 cut(s)	223, 1208, 3319, 3831
Cfr10I	RCCGGY	3 cut(s)	262, 287, 4165
Cfr42I	CCGCGG	1 cut(s)	56
DraIII	CACNNNGTG	1 cut(s)	2369
EaeI	YGGCCR	1 cut(s)	4182
Eam1104I	CTCTTC	6 cut(s)	546, 759, 1191, 1511, 2124, 3663
EarI	CTCTTC	6 cut(s)	546, 759, 1191, 1511, 2124, 3663
EciI	GGCGGA	1 cut(s)	545
Ecl136II	GAGCTC	1 cut(s)	1760
Eco130I	CCWWGG	1 cut(s)	2176
Eco24I	GRGCYC	1 cut(s)	1762
Eco31I	GGTCTC	2 cut(s)	1354, 2657
Eco32I	GATATC	1 cut(s)	3677
Eco47I	GGWCC	5 cut(s)	776, 1244, 1791, 2393, 4237
Eco53kI	GAGCTC	1 cut(s)	1760
Eco57I	CTGAAG	5 cut(s)	975, 1139, 2285, 2711, 3180
Eco88I	CYCGRG	2 cut(s)	491, 3233
EcoICRI	GAGCTC	1 cut(s)	1760
EcoO109I	RGGNCCY	3 cut(s)	1244, 2393, 3070
EcoRI	GAATTC	2 cut(s)	1609, 3610
EcoRII	CCWGG	5 cut(s)	8, 1522, 1870, 2081, 2572
EcoRV	GATATC	1 cut(s)	3677
EcoT14I	CCWWGG	1 cut(s)	2176
EcoT22I	ATGCAT	1 cut(s)	2647
EcoT38I	GRGCYC	1 cut(s)	1762
ErhI	CCWWGG	1 cut(s)	2176
FaqI	GGGAC	4 cut(s)	2109, 3356, 3487, 4250
FriOI	GRGCYC	1 cut(s)	1762
FspBI	CTAG	7 cut(s)	1190, 2022, 2562, 3438, 3711, 3807, 4158
GlaI	GCGC	4 cut(s)	222, 1207, 3318, 3830
GsaI	CCCAGC	2 cut(s)	1286, 1985
GsuI	CTGGAG	2 cut(s)	1881, 3115
HhaI	GCGC	4 cut(s)	223, 1208, 3319, 3831
Hin6I	GCGC	4 cut(s)	221, 1206, 3317, 3829
HinP1I	GCGC	4 cut(s)	221, 1206, 3317, 3829
HincII	GTYRAC	3 cut(s)	1441, 2935, 3661
HindII	GTYRAC	3 cut(s)	1441, 2935, 3661
HindIII	AAGCTT	3 cut(s)	920, 1556, 1829
HpaI	GTTAAC	1 cut(s)	2935
HphI	GGTGA	8 cut(s)	173, 553, 945, 1330, 2861, 3241, 3866, 4016
Hpy166II	GTNNAC	7 cut(s)	129, 1441, 1587, 2935, 3101, 3661, 4071
Hpy8I	GTNNAC	7 cut(s)	129, 1441, 1587, 2935, 3101, 3661, 4071
Hpy99I	CGWCG	5 cut(s)	79, 124, 136, 139, 3090
HpyCH4IV	ACGT	4 cut(s)	131, 2131, 3085, 3433
HpySE526I	ACGT	4 cut(s)	131, 2131, 3085, 3433
HspAI	GCGC	4 cut(s)	221, 1206, 3317, 3829
Kpn2I	TCCGGA	1 cut(s)	883
KpnI	GGTACC	1 cut(s)	3555
KroI	GCCGGC	1 cut(s)	262
KroNI	GCCGGC	1 cut(s)	264
KspAI	GTTAAC	1 cut(s)	2935
KspI	CCGCGG	1 cut(s)	56
LguI	GCTCTTC	2 cut(s)	1191, 2124
LmnI	GCTCC	7 cut(s)	49, 532, 1291, 1336, 1862, 2422, 3025
MaeI	CTAG	7 cut(s)	1190, 2022, 2562, 3438, 3711, 3807, 4158
MaeII	ACGT	4 cut(s)	131, 2131, 3085, 3433
MfeI	CAATTG	1 cut(s)	2907
MflI	RGATCY	7 cut(s)	592, 1027, 1141, 1159, 2790, 2809, 4270
MhlI	GDGCHC	8 cut(s)	259, 492, 791, 1186, 1762, 2119, 2191, 3401
MlsI	TGGCCA	1 cut(s)	4184
MluNI	TGGCCA	1 cut(s)	4184
MlyI	GAGTC	9 cut(s)	680, 935, 1499, 1528, 1969, 2091, 2852, 3584, 3698
MmeI	TCCRAC	7 cut(s)	405, 1005, 1191, 1674, 1693, 2310, 3340
Mox20I	TGGCCA	1 cut(s)	4184
Mph1103I	ATGCAT	1 cut(s)	2647
MroI	TCCGGA	1 cut(s)	883
MroNI	GCCGGC	1 cut(s)	262
MroXI	GAANNNNTTC	3 cut(s)	3177, 3634, 4230
MscI	TGGCCA	1 cut(s)	4184
MslI	CAYNNNNRTG	5 cut(s)	1496, 1918, 2112, 3447, 3944
Msp20I	TGGCCA	1 cut(s)	4184
MspA1I	CMGCKG	5 cut(s)	55, 663, 1981, 2137, 4264
MspCI	CTTAAG	1 cut(s)	3130
MspR9I	CCNGG	8 cut(s)	10, 646, 1139, 1524, 1872, 2083, 2574, 3421
MunI	CAATTG	1 cut(s)	2907
Mva1269I	GAATGC	4 cut(s)	574, 1333, 2647, 2991
MvaI	CCWGG	5 cut(s)	10, 1524, 1872, 2083, 2574
MvnI	CGCG	2 cut(s)	55, 1208
NaeI	GCCGGC	1 cut(s)	264
NciI	CCSGG	3 cut(s)	646, 1139, 3421
NgoMIV	GCCGGC	1 cut(s)	262
NmeAIII	GCCGAG	2 cut(s)	238, 961
NmuCI	GTSAC	6 cut(s)	161, 683, 732, 1928, 3089, 4201
NsiI	ATGCAT	1 cut(s)	2647
NspI	RCATGY	6 cut(s)	751, 862, 1789, 2219, 3308, 3708
OliI	CACNNNNGTG	1 cut(s)	1496
PaeI	GCATGC	1 cut(s)	2219
PaeR7I	CTCGAG	1 cut(s)	3233
PciI	ACATGT	1 cut(s)	3304
PciSI	GCTCTTC	2 cut(s)	1191, 2124
PcsI	WCGNNNNNNNCGW	2 cut(s)	128, 3057
PctI	GAATGC	4 cut(s)	574, 1333, 2647, 2991
PdiI	GCCGGC	1 cut(s)	264
PdmI	GAANNNNTTC	3 cut(s)	3177, 3634, 4230
PfeI	GAWTC	9 cut(s)	404, 512, 722, 2254, 3173, 3511, 3635, 3800, 4231
PflMI	CCANNNNNTGG	2 cut(s)	3568, 4117
PfoI	TCCNGGA	1 cut(s)	1137
PleI	GAGTC	9 cut(s)	680, 935, 1499, 1527, 1969, 2091, 2852, 3584, 3697
PpsI	GAGTC	9 cut(s)	680, 935, 1499, 1527, 1969, 2091, 2852, 3584, 3697
PpuMI	RGGWCCY	2 cut(s)	1244, 2393
PscI	ACATGT	1 cut(s)	3304
PsiI	TTATAA	1 cut(s)	2771
Psp124BI	GAGCTC	1 cut(s)	1762
Psp5II	RGGWCCY	2 cut(s)	1244, 2393
Psp6I	CCWGG	5 cut(s)	8, 1522, 1870, 2081, 2572
PspFI	CCCAGC	2 cut(s)	1282, 1981
PspGI	CCWGG	5 cut(s)	8, 1522, 1870, 2081, 2572
PspPPI	RGGWCCY	2 cut(s)	1244, 2393
PstI	CTGCAG	2 cut(s)	1102, 1603
PsuI	RGATCY	7 cut(s)	592, 1027, 1141, 1159, 2790, 2809, 4270
PvuII	CAGCTG	3 cut(s)	1981, 2137, 4264
RseI	CAYNNNNRTG	5 cut(s)	1496, 1918, 2112, 3447, 3944
SacI	GAGCTC	1 cut(s)	1762
SacII	CCGCGG	1 cut(s)	56
SapI	GCTCTTC	2 cut(s)	1191, 2124
SbfI	CCTGCAGG	1 cut(s)	1102
ScaI	AGTACT	1 cut(s)	2803
SchI	GAGTC	9 cut(s)	680, 935, 1499, 1528, 1969, 2091, 2852, 3584, 3698
ScrFI	CCNGG	8 cut(s)	10, 646, 1139, 1524, 1872, 2083, 2574, 3421
SdaI	CCTGCAGG	1 cut(s)	1102
SduI	GDGCHC	8 cut(s)	259, 492, 791, 1186, 1762, 2119, 2191, 3401
SfcI	CTRYAG	4 cut(s)	1098, 1371, 1599, 3931
Sfr274I	CTCGAG	1 cut(s)	3233
Sfr303I	CCGCGG	1 cut(s)	56
SgrBI	CCGCGG	1 cut(s)	56
SinI	GGWCC	5 cut(s)	776, 1244, 1791, 2393, 4237
SlaI	CTCGAG	1 cut(s)	3233
SmiMI	CAYNNNNRTG	5 cut(s)	1496, 1918, 2112, 3447, 3944
SmlI	CTYRAG	9 cut(s)	943, 1383, 1633, 1808, 2654, 2737, 2996, 3130, 3233
SmoI	CTYRAG	9 cut(s)	943, 1383, 1633, 1808, 2654, 2737, 2996, 3130, 3233
SphI	GCATGC	1 cut(s)	2219
Sse8387I	CCTGCAGG	1 cut(s)	1102
SspMI	CTAG	7 cut(s)	1190, 2022, 2562, 3438, 3711, 3807, 4158
SstI	GAGCTC	1 cut(s)	1762
StyD4I	CCNGG	8 cut(s)	8, 644, 1137, 1522, 1870, 2081, 2572, 3419
StyI	CCWWGG	1 cut(s)	2176
TaiI	ACGT	4 cut(s)	134, 2134, 3088, 3436
TaqI	TCGA	4 cut(s)	122, 3060, 3234, 3608
TatI	WGTACW	6 cut(s)	1114, 2801, 2976, 3927, 4068, 4086
TauI	GCSGC	9 cut(s)	55, 58, 89, 92, 95, 666, 989, 1806, 3017
TfiI	GAWTC	9 cut(s)	404, 512, 722, 2254, 3173, 3511, 3635, 3800, 4231
TscAI	CASTG	4 cut(s)	741, 796, 1654, 2763
TseFI	GTSAC	6 cut(s)	161, 683, 732, 1928, 3089, 4201
Tsp45I	GTSAC	6 cut(s)	161, 683, 732, 1928, 3089, 4201
TspGWI	ACGGA	5 cut(s)	262, 1541, 2302, 2870, 4088
TspRI	CASTG	4 cut(s)	741, 796, 1654, 2763
Van91I	CCANNNNNTGG	2 cut(s)	3568, 4117
Vha464I	CTTAAG	1 cut(s)	3130
VpaK11BI	GGWCC	5 cut(s)	776, 1244, 1791, 2393, 4237
XapI	RAATTY	8 cut(s)	1267, 1310, 1609, 2530, 2599, 3138, 3610, 4188
XceI	RCATGY	6 cut(s)	751, 862, 1789, 2219, 3308, 3708
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1785, 4204
XhoI	CTCGAG	1 cut(s)	3233
XmnI	GAANNNNTTC	3 cut(s)	3177, 3634, 4230
XspI	CTAG	7 cut(s)	1190, 2022, 2562, 3438, 3711, 3807, 4158
ZrmI	AGTACT	1 cut(s)	2803
Zsp2I	ATGCAT	1 cut(s)	2647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.