Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Forward (+)

25996571 .. 26007300

Gene Structure 10730 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1285400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4602 bp

ATGGCGGACGACGAGCAAGCGACGGTGGCCACCAGCAGGCGGCGGAGTCGAGGACCAGAGGCGTCGGCCCGTGCCGGAGCCCTCGAACGCCTCAAAGCTCTTCGCCATGGTGGCCGCCGATCTGAAGCTGGTGGGTTCCAAATCAAGATGGAGGACCGAATCTATGACACTGTCGGAGACGACGAGTACGACGCAATCGTCGCCAAACGACGGGAAGAGGTCCGAGGCTTCATCGTCGACGACAACGGCCTAGGTTACTGCGATGAGGGCGAGGAAGAAGACTGGACTCGTGCCGGTCCGTCGTCGGATGAATCGGACGGCGGCGATAGGCCCAAGAGGAAGAAAGTCGAAAAAAAGGAAAAGGAAAAGGACAAAGAGCCTCGGCCCAAAAAGCCCAGTTCGTCGCTCACTGCCGCGGCGGCGATGATGGGGAAGCAGAGGCTCTCGTCGATGTTCACGTCGTCGGTTTTCGGGAAAAATAGAGATAATGAGAGGGGGAGAGGGTTGAATTGTGATAGTATTGTGGATGATGTGATTGCGGAGTTTGCACCAGATGAGGATGACAGAGAGAAGCGGAGGAGGGGGCAATCAAACAAGATTTTGGTGCCGAATTTGAGTAATAAGAGTGAAATTGGGTTTGATAGGGGTGTTAATTTAGCAAGGAAGGTTGAATTGGATAGAGTTGTGGCAAATAGTAGTGATTCTTTGGTGGGTTCTGTGCAGATTAATGAAGGATTTGAAGAAAATGAGGGCAAGAAGGAGGTGGGTTTAGAAGGAGGTGATCCCCCAATTGAGCAAAGCCATGAATTTTCTGAGGGTGGAGGGGCAATTGAGGAGAAAAATGTGGATTTTGTTGAGGAGAAAGTGGAACCTGTGAAGAAAAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATAACAAGGACCCGGCATTGAGTGCTATGGTGGGATGGAAGGCGGTGAGGACTGGAGGTGATGGGAATGTTGGTGGTGGTGTTGTGGAGGCCAATAGCGGCTCAAATAGTGAAGAGATTTCGCAGTGTGATTTGGAAGAAGATGGTTCACTGCTGTTTTACATGATTGATGCTTATGAAGAGTTCTATGGTGCTAATGGGGGCAAGCTTTATCTGTTTGGGAAGGTCAAAGCAGGAAGTACATACCAGAGTTGTTGTGTGGTTGTAGAGAACATGCAGAGATGTGTATACGCAATTCCAAAGAGTTCTGTATTTCACAGTGATGAAATCATGAAGCTTGAAAAGGATGCCGAAGAGTCGAGGATTTCATCCAGAGAATTCCGTACAAAGCTGCATGATATGGCTTCTGGTTTAAAGAGCGACATGGCAAAGAAGTTGTTGGATCTCAATATATCAGCTTTTAGCATGGCGCTGGTTAAGAGGAGCTATGCATTTGAGCGATCTGACATACCTGCAGGAGAGAATTATGTACTTAAGATCAATTATCCATTCAAGGATCCCCCACTTCCAGCAGATCTTAAAGGAGAAACATTGCTTGCTCTTCTAGGAACACATACCAGCGCTTTGGAGCTTTTTCTTGTTAAAAGGAAGATAAAAGGCCCGTCTTGGTTATCAGTTTCAGGTTCAGAGTTTCGTCCAGTTTCACAACGGGTAAGTTGGTGCAAATTTGAGGTGGTTGTTAAATCTGCAAAAGACATACAGGTCTCAAATTGCCCAAAGAAAACTGCAGAGGCTCCCCCAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTAATTTTGACGAAATTGTCTCTGCATCTGTTATCTGCTGTCACAAGGCCAAGATTGACACTCCTGTGTTAGCATCGGAGTGGACAAAACCTGGCATGCTGAGCCATTTTACTGTTGTCCGTAAACTTGATGGAGGCATATTTCCAATGGGCTTCACTAAGGAGGCTACAGAAAAGAATTCTAAAGCTGGATCAAATGTTTTAAGCATCGAAAGCACTGAAAGGGCTTTACTAAATCGTTTGATGATTGAATTACACAAGTTGGATAGTGATGTTCTTGTTGGACACAATATATCTGGGTTTGACCTGGATGTCCTTCTCCGCAGGGCTAAGGTTTGTAAGGTGCCAAACTCCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGGGATGCCTAACCTTGCAAAAGGAAGCTCCATTTTTGGGTCTGGAGCAAGTCTAGGGATCTTGTCCTGCATTTCTGGTCGCCTTTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAGGGAGGTTAGCTATTCTTTGACACAACTTGCAAAGACTCAGCTGAATAAAGACCGAAAGGAGATCATGCCACATGATATTCCTAGAATGTTCCAGAAATCAGAATTTCTAATGGAACTGATTGAATATGGCGAGACAGATGCATGGCTGTCTATGGAACTCATGTTTCATTTGAGCGTTCTTCCCCTTACACGTCAGCTGACAAAGATAAGCGGTAATCTGTGGGGAAAAACCCTACAAGGTGCTAGGGCACAAAGAGTAGAATATCTTTTACTGCATGCCTTCCATGCAAAGAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGAAACAATTTCGGCAAAGCGTAAAACAGATAATGGTGTTGATGATAAAAATGTTGATGAGGTGGATGTAAATTTTGATAATGAGGCAAAGAAGGATCATGGGAAAGGCAAGAAAGGTCCTGCCTATGCAGGTGGATTGGTATTGGAGCCAAAAAGAGGGTTATATGACAAATATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATCTGTTTCACAACAGTGGAAAGATCTTCAGAAGGATTGGCTCCTCGTTTACCATCTAGTAAAAAAATTGGGCTTCTACCTGAGTTGTTAAAAGATCTAGTTGAAAGGAGGAGAAATGTAAAGAAATGGATGAAGACTGCATCTGGTCTCAAGATCCAGCAACTTGACATTCAACAGCAAGCTCTAAAGCTGACTGCAAATAGTATGTATGGATGCTTGGGGTTTCCAAATTCGAGATTTTATGCAAAGCCACTGGCTGAGCTTATAACACTGCAAGGGAGAGAGATTTTGCAAAGTACCGTAGATCTTGTTCAGAACAATTTGAACCTAGAGGTAATCTACGGTGATACTGATTCAATCATGATTTATAGTGGACTGGATGATATTGCAAAAGCAAAAGCAATTGCATTGAAAGTCATCCAAGAGGTTAACAAGAAGTATAGGTGTTTGGAAATTGATCTTGATGGGTTATACAAGAGAATGCTGCTTCTTAAGAAAAAGAAATATGCAGCTGTGAAGGTGCAATTCAAGAATGAGATGCCCTATGAGGTTATTGAGCGTAAGGGTCTTGATATGGTTCGACGGGACTGGAGCTTACTTTCAAAGGAACTGGGAGATTTCTGCCTAAGTAAAATTTTATCAGGAGGGTCATGCGAGGATGTTGTTGAATCAATTCATGACGCTCTGATGAAGGTGCAAGAGGAGATGAGAAACGGAGAAGTAGCACTTGAAAAATATGTCATCACCAAGTCATTGACTAAACCACCTGAAGCATACCCAGATGCCAAAAACCAACCACATGTTCAGGTGGCATTAAGATTAAAGCAAAGTGGTTATTCTGCTGGCTGTTCTGCTGGCGATACAGTCCCCTATATCATTTGCTGTGAGCAGGGGACTAGTATAAGTAATTCAACGGGGATTGCTCAACGTGCTCGACATCCGGATGAATTAAAACGAGAAGATGGAAAATGGATGGTTGACATTGATTACTACTTGTCCCAGCAGATTCATCCTGTGGTATCACGTCTTTGTGCTTCGATTCAGGGTACCAGCCCCGGACGCTTGGCTGATTGTTTAGGACTTGACTCTTCTAAGTTCAAAGGTAACTCAAGTGAAGCTGTCAAAGATGATCCTTCCAGCTTGTCCCTTCTTCTTGATGATGCAGAGAGATTTTGCAGTTGTGAGCCTTTGATCTTGGCATGTCCTAGCTGCTCCAATACTTTTAGTTGTCCAACTATCTTGAATTCCATTTCTAAGTCAATTACCGAAAAGCAAAGTAAGTCACAAGCTGAGGAATCTACCAGCGATTTTTGGCATACACTACGCTGTTCAAAATGCCCGGGGGAAGGAGACATGGGCAGACTGTCTCCTGTAATGATAGCCAACCAGGTGAAAAGGCAAGCAGACGGTTTTGTTTCAATGTACTACAGGGGCTTTATGACGTGTGACGACGAATCTTGCCAATACAACACACGAAGTCTCAACCTCCGGCTTGTTGGCGATGCTGAGAGAGGAACGGTTTGTCCGGACTATCCTCGCTGCAACGGACGTCTTGTAAGAAAGTACACGGAAGCAGATCTGTACAAGCAGCTCTCCTTTTTCTGCCACATCTTGGATACAGAACGCTGCATAGAAAAGATGGAGGCTAGCACTAGACTGCCGGTAGAGATGGAGTTGGCCAAAGTTCGGCCAATCGCGGATTTGGCTGCTTCAACAGTTAAAAGGATTCGGGACCGTTGTGCCTACGGATGGATACAGCTGCAAGATCTTTGTGTTACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1534

Amino Acids

170.74

Weight (kDa)

7.54

Isoelectric Point (pI)

45.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	28 - 95	2.5e-19	DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1	PF03104	511 - 761	1.8e-26	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	827 - 1286	4e-125	DNA polymerase family B
zf-DNA_Pol	PF08996	1324 - 1529	2.9e-40	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3083
AarI	CACCTGC	1 cut(s)	2708
AasI	GACNNNNNNGTC	4 cut(s)	197, 1682, 1769, 2063
AatII	GACGTC	1 cut(s)	4369
Acc36I	ACCTGC	2 cut(s)	1441, 2708
Acc65I	GGTACC	1 cut(s)	3863
AccB1I	GGYRCC	3 cut(s)	604, 2095, 3863
AccB7I	CCANNNNNTGG	1 cut(s)	4429
AccI	GTMKAC	2 cut(s)	237, 1209
AccII	CGCG	2 cut(s)	416, 4514
AccIII	TCCGGA	2 cut(s)	3757, 4342
AclWI	GGATC	9 cut(s)	776, 1371, 1472, 1485, 1951, 2204, 2691, 2965, 3941
AcoI	YGGCCR	4 cut(s)	27, 112, 4494, 4505
AcuI	CTGAAG	4 cut(s)	144, 2606, 2829, 3606
AcyI	GRCGYC	2 cut(s)	62, 4366
AfaI	GTAC	9 cut(s)	188, 1162, 1306, 1452, 3115, 3865, 4241, 4382, 4400
AfeI	AGCGCT	1 cut(s)	1543
AfiI	CCNNNNNNNGG	9 cut(s)	36, 39, 210, 2175, 2744, 4163, 4429, 4503, 4566
AflII	CTTAAG	2 cut(s)	1454, 3308
AflIII	ACRYGT	3 cut(s)	2450, 3616, 4259
AhlI	ACTAGT	1 cut(s)	3713
AjiI	CACGTC	4 cut(s)	459, 2453, 3842, 4260
AjnI	CCWGG	3 cut(s)	1843, 2058, 4203
AjuI	GAANNNNNNNTTGG	4 cut(s)	656, 688, 3603, 3635
AleI	CACNNNNGTG	2 cut(s)	1817, 2831
AloI	GAACNNNNNNTCC	2 cut(s)	4324, 4356
Alw21I	GWGCWC	1 cut(s)	3751
Alw26I	GTCTC	8 cut(s)	171, 1690, 1777, 2387, 2969, 4161, 4188, 4301
AlwI	GGATC	9 cut(s)	776, 1371, 1472, 1485, 1951, 2204, 2691, 2965, 3941
Ama87I	CYCGRG	1 cut(s)	4156
Aor13HI	TCCGGA	2 cut(s)	3757, 4342
Aor51HI	AGCGCT	1 cut(s)	1543
ApeKI	GCWGC	9 cut(s)	1312, 3301, 3326, 4026, 4356, 4405, 4443, 4523, 4576
AseI	ATTAAT	2 cut(s)	726, 1755
Asp700I	GAANNNNTTC	1 cut(s)	3489
Asp718I	GGTACC	1 cut(s)	3863
AspA2I	CCTAGG	1 cut(s)	250
AspLEI	GCGC	2 cut(s)	1393, 1544
AsuC2I	CCSGG	4 cut(s)	935, 3873, 4157, 4158
AsuHPI	GGTGA	6 cut(s)	791, 979, 992, 3173, 3553, 4219
AsuNHI	GCTAGC	1 cut(s)	4463
AvaI	CYCGRG	1 cut(s)	4156
AvaII	GGWCC	8 cut(s)	53, 154, 220, 296, 931, 2112, 2705, 4549
AvrII	CCTAGG	1 cut(s)	250
BaeGI	GKGCMC	1 cut(s)	2512
BalI	TGGCCA	2 cut(s)	29, 4496
BamHI	GGATCC	1 cut(s)	1477
BanI	GGYRCC	3 cut(s)	604, 2095, 3863
BanII	GRGCYC	1 cut(s)	82
BauI	CACGAG	1 cut(s)	288
BbsI	GAAGAC	2 cut(s)	285, 2957
Bbv12I	GWGCWC	1 cut(s)	3751
BbvCI	CCTCAGC	1 cut(s)	4107
BbvI	GCAGC	9 cut(s)	1299, 3288, 3338, 4013, 4343, 4417, 4430, 4510, 4563
BceAI	ACGGC	2 cut(s)	262, 334
BcgI	CGANNNNNNTGC	2 cut(s)	2381, 2415
BciT130I	CCWGG	3 cut(s)	1845, 2060, 4205
BciVI	GTATCC	2 cut(s)	4426, 4563
BcnI	CCSGG	4 cut(s)	935, 3873, 4157, 4158
BcoDI	GTCTC	8 cut(s)	171, 1690, 1777, 2387, 2969, 4161, 4188, 4301
BcuI	ACTAGT	1 cut(s)	3713
BfmI	CTRYAG	4 cut(s)	1434, 1707, 1920, 4243
BfoI	RGCGCY	2 cut(s)	1394, 1545
BfrI	CTTAAG	2 cut(s)	1454, 3308
BfuAI	ACCTGC	2 cut(s)	1441, 2708
BfuI	GTATCC	2 cut(s)	4426, 4563
BglI	GCCNNNNNGGC	2 cut(s)	111, 419
BglII	AGATCT	6 cut(s)	1495, 2840, 2911, 3121, 4393, 4582
BlnI	CCTAGG	1 cut(s)	250
BlpI	GCTNAGC	2 cut(s)	1853, 3075
Bme1390I	CCNGG	7 cut(s)	935, 1845, 2060, 3873, 4157, 4158, 4205
Bme18I	GGWCC	8 cut(s)	53, 154, 220, 296, 931, 2112, 2705, 4549
BmeT110I	CYCGRG	1 cut(s)	4156
BmgBI	CACGTC	4 cut(s)	459, 2453, 3842, 4260
BmrFI	CCNGG	7 cut(s)	935, 1845, 2060, 3873, 4157, 4158, 4205
BmrI	ACTGGG	3 cut(s)	390, 1715, 3437
BmtI	GCTAGC	1 cut(s)	4467
BmuI	ACTGGG	3 cut(s)	390, 1715, 3437
BpiI	GAAGAC	2 cut(s)	285, 2957
BpmI	CTGGAG	3 cut(s)	996, 2202, 3427
Bpu10I	CCTNAGC	2 cut(s)	2082, 4107
Bpu1102I	GCTNAGC	2 cut(s)	1853, 3075
BpuEI	CTTGAG	3 cut(s)	2114, 2951, 3910
BpuMI	CCSGG	4 cut(s)	935, 3873, 4157, 4158
BsaHI	GRCGYC	2 cut(s)	62, 4366
BsaI	GGTCTC	2 cut(s)	1690, 2969
BsaJI	CCNNGG	8 cut(s)	106, 223, 250, 380, 414, 3871, 4156, 4157
BsaWI	WCCGGW	2 cut(s)	3757, 4342
Bsc4I	CCNNNNNNNGG	9 cut(s)	36, 39, 210, 2175, 2744, 4163, 4429, 4503, 4566
Bse118I	RCCGGY	2 cut(s)	293, 4477
Bse1I	ACTGG	9 cut(s)	287, 396, 979, 1619, 1721, 3075, 3198, 3410, 3432
Bse3DI	GCAATG	1 cut(s)	1511
BseAI	TCCGGA	2 cut(s)	3757, 4342
BseBI	CCWGG	3 cut(s)	1845, 2060, 4205
BseDI	CCNNGG	8 cut(s)	106, 223, 250, 380, 414, 3871, 4156, 4157
BseLI	CCNNNNNNNGG	9 cut(s)	36, 39, 210, 2175, 2744, 4163, 4429, 4503, 4566
BseMI	GCAATG	1 cut(s)	1511
BseMII	CTCAG	7 cut(s)	804, 1844, 2312, 2889, 3066, 4098, 4314
BseNI	ACTGG	9 cut(s)	287, 396, 979, 1619, 1721, 3075, 3198, 3410, 3432
BseRI	GAGGAG	7 cut(s)	592, 848, 872, 1417, 2850, 2941, 3533
BseSI	GKGCMC	1 cut(s)	2512
BseXI	GCAGC	9 cut(s)	1299, 3288, 3338, 4013, 4343, 4417, 4430, 4510, 4563
BseYI	CCCAGC	1 cut(s)	3816
BsgI	GTGCAG	1 cut(s)	740
Bsh1236I	CGCG	2 cut(s)	416, 4514
BshNI	GGYRCC	3 cut(s)	604, 2095, 3863
BsiHKAI	GWGCWC	1 cut(s)	3751
BsiHKCI	CYCGRG	1 cut(s)	4156
BsiSI	CCGG	9 cut(s)	75, 294, 935, 3758, 3873, 4157, 4306, 4343, 4478
BslFI	GGGAC	6 cut(s)	3416, 3668, 3724, 3799, 3946, 4562
BslI	CCNNNNNNNGG	9 cut(s)	36, 39, 210, 2175, 2744, 4163, 4429, 4503, 4566
BsmAI	GTCTC	8 cut(s)	171, 1690, 1777, 2387, 2969, 4161, 4188, 4301
BsmBI	CGTCTC	1 cut(s)	171
BsmFI	GGGAC	6 cut(s)	3416, 3668, 3724, 3799, 3946, 4562
BsmI	GAATGC	2 cut(s)	910, 3303
Bso31I	GGTCTC	2 cut(s)	1690, 2969
BsoBI	CYCGRG	1 cut(s)	4156
Bsp1286I	GDGCHC	3 cut(s)	82, 2512, 3751
Bsp13I	TCCGGA	2 cut(s)	3757, 4342
Bsp1407I	TGTACA	1 cut(s)	4398
Bsp1720I	GCTNAGC	2 cut(s)	1853, 3075
Bsp19I	CCATGG	1 cut(s)	106
BspCNI	CTCAG	7 cut(s)	805, 1845, 2311, 2890, 3067, 4099, 4315
BspEI	TCCGGA	2 cut(s)	3757, 4342
BspFNI	CGCG	2 cut(s)	416, 4514
BspHI	TCATGA	3 cut(s)	1251, 3177, 3493
BspMAI	CTGCAG	2 cut(s)	1438, 1711
BspMI	ACCTGC	2 cut(s)	1441, 2708
BspOI	GCTAGC	1 cut(s)	4467
BspPI	GGATC	9 cut(s)	776, 1371, 1472, 1485, 1951, 2204, 2691, 2965, 3941
BspQI	GCTCTTC	2 cut(s)	105, 1527
BspT107I	GGYRCC	3 cut(s)	604, 2095, 3863
BspTI	CTTAAG	2 cut(s)	1454, 3308
BspTNI	GGTCTC	2 cut(s)	1690, 2969
BsrDI	GCAATG	1 cut(s)	1511
BsrFI	RCCGGY	2 cut(s)	293, 4477
BsrGI	TGTACA	1 cut(s)	4398
BsrI	ACTGG	9 cut(s)	287, 396, 979, 1619, 1721, 3075, 3198, 3410, 3432
BssAI	RCCGGY	2 cut(s)	293, 4477
BssECI	CCNNGG	8 cut(s)	106, 223, 250, 380, 414, 3871, 4156, 4157
BssNAI	GTATAC	1 cut(s)	1210
BssNI	GRCGYC	2 cut(s)	62, 4366
BssSI	CACGAG	1 cut(s)	288
BssT1I	CCWWGG	2 cut(s)	106, 250
Bst1107I	GTATAC	1 cut(s)	1210
Bst2BI	CACGAG	1 cut(s)	288
Bst2UI	CCWGG	3 cut(s)	1845, 2060, 4205
Bst6I	CTCTTC	8 cut(s)	105, 210, 882, 1029, 1095, 1269, 1527, 3910
BstACI	GRCGYC	2 cut(s)	62, 4366
BstAFI	CTTAAG	2 cut(s)	1454, 3308
BstAPI	GCANNNNNTGC	1 cut(s)	944
BstAUI	TGTACA	1 cut(s)	4398
BstDSI	CCRYGG	2 cut(s)	106, 414
BstFNI	CGCG	2 cut(s)	416, 4514
BstH2I	RGCGCY	2 cut(s)	1394, 1545
BstHHI	GCGC	2 cut(s)	1393, 1544
BstMAI	GTCTC	8 cut(s)	171, 1690, 1777, 2387, 2969, 4161, 4188, 4301
BstNI	CCWGG	3 cut(s)	1845, 2060, 4205
BstNSI	RCATGY	5 cut(s)	1198, 1852, 2540, 3620, 4020
BstSCI	CCNGG	7 cut(s)	933, 1843, 2058, 3871, 4155, 4156, 4203
BstSFI	CTRYAG	4 cut(s)	1434, 1707, 1920, 4243
BstSLI	GKGCMC	1 cut(s)	2512
BstUI	CGCG	2 cut(s)	416, 4514
BstV1I	GCAGC	9 cut(s)	1299, 3288, 3338, 4013, 4343, 4417, 4430, 4510, 4563
BstV2I	GAAGAC	2 cut(s)	285, 2957
BstXI	CCANNNNNNTGG	1 cut(s)	1546
BstZ17I	GTATAC	1 cut(s)	1210
BsuI	GTATCC	2 cut(s)	4426, 4563
BtgI	CCRYGG	2 cut(s)	106, 414
BtgZI	GCGATG	3 cut(s)	276, 437, 4332
BtrI	CACGTC	4 cut(s)	459, 2453, 3842, 4260
BtsI	GCAGTG	4 cut(s)	408, 1052, 1070, 3086
BveI	ACCTGC	2 cut(s)	1441, 2708
CciI	TCATGA	3 cut(s)	1251, 3177, 3493
CfoI	GCGC	2 cut(s)	1393, 1544
Cfr10I	RCCGGY	2 cut(s)	293, 4477
Cfr42I	CCGCGG	1 cut(s)	417
Cfr9I	CCCGGG	1 cut(s)	4156
CpoI	CGGWCCG	1 cut(s)	296
CseI	GACGC	4 cut(s)	51, 200, 3506, 3885
CsiI	ACCWGGT	1 cut(s)	4203
Csp6I	GTAC	9 cut(s)	187, 1161, 1305, 1451, 3114, 3864, 4240, 4381, 4399
CspI	CGGWCCG	1 cut(s)	296
CviQI	GTAC	9 cut(s)	187, 1161, 1305, 1451, 3114, 3864, 4240, 4381, 4399
DraI	TTTAAA	1 cut(s)	1335
DrdI	GACNNNNNNGTC	4 cut(s)	197, 1682, 1769, 2063
DseDI	GACNNNNNNGTC	4 cut(s)	197, 1682, 1769, 2063
EaeI	YGGCCR	4 cut(s)	27, 112, 4494, 4505
Eam1104I	CTCTTC	8 cut(s)	105, 210, 882, 1029, 1095, 1269, 1527, 3910
EarI	CTCTTC	8 cut(s)	105, 210, 882, 1029, 1095, 1269, 1527, 3910
EciI	GGCGGA	2 cut(s)	20, 58
Eco130I	CCWWGG	2 cut(s)	106, 250
Eco24I	GRGCYC	1 cut(s)	82
Eco31I	GGTCTC	2 cut(s)	1690, 2969
Eco47I	GGWCC	8 cut(s)	53, 154, 220, 296, 931, 2112, 2705, 4549
Eco47III	AGCGCT	1 cut(s)	1543
Eco57I	CTGAAG	4 cut(s)	144, 2606, 2829, 3606
Eco88I	CYCGRG	1 cut(s)	4156
EcoO109I	RGGNCCY	2 cut(s)	931, 2705
EcoRI	GAATTC	3 cut(s)	1298, 1930, 4060
EcoRII	CCWGG	3 cut(s)	1843, 2058, 4203
EcoT14I	CCWWGG	2 cut(s)	106, 250
EcoT22I	ATGCAT	2 cut(s)	1414, 2404
EcoT38I	GRGCYC	1 cut(s)	82
ErhI	CCWWGG	2 cut(s)	106, 250
Esp3I	CGTCTC	1 cut(s)	171
FalI	AAGNNNNNCTT	2 cut(s)	3528, 3560
FaqI	GGGAC	6 cut(s)	3416, 3668, 3724, 3799, 3946, 4562
FblI	GTMKAC	2 cut(s)	237, 1209
FriOI	GRGCYC	1 cut(s)	82
GlaI	GCGC	2 cut(s)	1392, 1543
GsaI	CCCAGC	1 cut(s)	3820
GsuI	CTGGAG	3 cut(s)	996, 2202, 3427
HaeII	RGCGCY	2 cut(s)	1394, 1545
HapII	CCGG	9 cut(s)	75, 294, 935, 3758, 3873, 4157, 4306, 4343, 4478
HgaI	GACGC	4 cut(s)	51, 200, 3506, 3885
HhaI	GCGC	2 cut(s)	1393, 1544
Hin1I	GRCGYC	2 cut(s)	62, 4366
Hin6I	GCGC	2 cut(s)	1391, 1542
HinP1I	GCGC	2 cut(s)	1391, 1542
HincII	GTYRAC	3 cut(s)	238, 3247, 3796
HindII	GTYRAC	3 cut(s)	238, 3247, 3796
HindIII	AAGCTT	2 cut(s)	1127, 1256
HpaI	GTTAAC	1 cut(s)	3247
HpaII	CCGG	9 cut(s)	75, 294, 935, 3758, 3873, 4157, 4306, 4343, 4478
HphI	GGTGA	6 cut(s)	791, 979, 992, 3173, 3553, 4219
HpyCH4IV	ACGT	6 cut(s)	458, 2452, 3745, 3841, 4259, 4366
HpySE526I	ACGT	6 cut(s)	458, 2452, 3745, 3841, 4259, 4366
Hsp92I	GRCGYC	2 cut(s)	62, 4366
HspAI	GCGC	2 cut(s)	1391, 1542
Kpn2I	TCCGGA	2 cut(s)	3757, 4342
KpnI	GGTACC	1 cut(s)	3867
KspAI	GTTAAC	1 cut(s)	3247
KspI	CCGCGG	1 cut(s)	417
LguI	GCTCTTC	2 cut(s)	105, 1527
Lsp1109I	GCAGC	9 cut(s)	1299, 3288, 3338, 4013, 4343, 4417, 4430, 4510, 4563
MabI	ACCWGGT	1 cut(s)	4203
MaeII	ACGT	6 cut(s)	458, 2452, 3745, 3841, 4259, 4366
MaeIII	GTNAC	8 cut(s)	254, 1729, 1793, 2249, 3920, 4098, 4262, 4591
MfeI	CAATTG	3 cut(s)	789, 828, 3219
MhlI	GDGCHC	3 cut(s)	82, 2512, 3751
MlsI	TGGCCA	2 cut(s)	29, 4496
MluNI	TGGCCA	2 cut(s)	29, 4496
MlyI	GAGTC	5 cut(s)	55, 280, 1286, 2290, 3896
MmeI	TCCRAC	6 cut(s)	154, 285, 1341, 1995, 2014, 4073
Mox20I	TGGCCA	2 cut(s)	29, 4496
Mph1103I	ATGCAT	2 cut(s)	1414, 2404
MroI	TCCGGA	2 cut(s)	3757, 4342
MroXI	GAANNNNTTC	1 cut(s)	3489
MscI	TGGCCA	2 cut(s)	29, 4496
MslI	CAYNNNNRTG	6 cut(s)	1242, 1817, 1831, 2239, 2831, 3759
Msp20I	TGGCCA	2 cut(s)	29, 4496
MspA1I	CMGCKG	5 cut(s)	416, 2302, 2458, 3329, 4576
MspCI	CTTAAG	2 cut(s)	1454, 3308
MspI	CCGG	9 cut(s)	75, 294, 935, 3758, 3873, 4157, 4306, 4343, 4478
MspR9I	CCNGG	7 cut(s)	935, 1845, 2060, 3873, 4157, 4158, 4205
MunI	CAATTG	3 cut(s)	789, 828, 3219
Mva1269I	GAATGC	2 cut(s)	910, 3303
MvaI	CCWGG	3 cut(s)	1845, 2060, 4205
MvnI	CGCG	2 cut(s)	416, 4514
NciI	CCSGG	4 cut(s)	935, 3873, 4157, 4158
NcoI	CCATGG	1 cut(s)	106
NheI	GCTAGC	1 cut(s)	4463
NmeAIII	GCCGAG	1 cut(s)	361
NmuCI	GTSAC	4 cut(s)	1793, 2249, 4098, 4262
NsiI	ATGCAT	2 cut(s)	1414, 2404
NspI	RCATGY	5 cut(s)	1198, 1852, 2540, 3620, 4020
OliI	CACNNNNGTG	2 cut(s)	1817, 2831
PaeI	GCATGC	2 cut(s)	1852, 2540
PagI	TCATGA	3 cut(s)	1251, 3177, 3493
PaqCI	CACCTGC	1 cut(s)	2708
PciI	ACATGT	1 cut(s)	3616
PciSI	GCTCTTC	2 cut(s)	105, 1527
PcsI	WCGNNNNNNNCGW	4 cut(s)	180, 186, 195, 455
PctI	GAATGC	2 cut(s)	910, 3303
PdmI	GAANNNNTTC	1 cut(s)	3489
PflFI	GACNNNGTC	1 cut(s)	170
PflMI	CCANNNNNTGG	1 cut(s)	4429
PleI	GAGTC	5 cut(s)	54, 280, 1285, 2290, 3896
PpsI	GAGTC	5 cut(s)	54, 280, 1285, 2290, 3896
PpuMI	RGGWCCY	2 cut(s)	931, 2705
PscI	ACATGT	1 cut(s)	3616
PshBI	ATTAAT	2 cut(s)	726, 1755
PsiI	TTATAA	1 cut(s)	3083
Psp5II	RGGWCCY	2 cut(s)	931, 2705
Psp6I	CCWGG	3 cut(s)	1843, 2058, 4203
PspFI	CCCAGC	1 cut(s)	3816
PspGI	CCWGG	3 cut(s)	1843, 2058, 4203
PspPPI	RGGWCCY	2 cut(s)	931, 2705
PsrI	GAACNNNNNNTAC	2 cut(s)	3111, 3143
PstI	CTGCAG	2 cut(s)	1438, 1711
PsyI	GACNNNGTC	1 cut(s)	170
PvuII	CAGCTG	4 cut(s)	2302, 2458, 3329, 4576
RsaI	GTAC	9 cut(s)	188, 1162, 1306, 1452, 3115, 3865, 4241, 4382, 4400
RsaNI	GTAC	9 cut(s)	187, 1161, 1305, 1451, 3114, 3864, 4240, 4381, 4399
RseI	CAYNNNNRTG	6 cut(s)	1242, 1817, 1831, 2239, 2831, 3759
Rsr2I	CGGWCCG	1 cut(s)	296
RsrII	CGGWCCG	1 cut(s)	296
SacII	CCGCGG	1 cut(s)	417
SalI	GTCGAC	1 cut(s)	236
SapI	GCTCTTC	2 cut(s)	105, 1527
SbfI	CCTGCAGG	1 cut(s)	1438
SchI	GAGTC	5 cut(s)	55, 280, 1286, 2290, 3896
ScrFI	CCNGG	7 cut(s)	935, 1845, 2060, 3873, 4157, 4158, 4205
SdaI	CCTGCAGG	1 cut(s)	1438
SduI	GDGCHC	3 cut(s)	82, 2512, 3751
SexAI	ACCWGGT	1 cut(s)	4203
SfcI	CTRYAG	4 cut(s)	1434, 1707, 1920, 4243
Sfr303I	CCGCGG	1 cut(s)	417
SgrBI	CCGCGG	1 cut(s)	417
SgrDI	CGTCGACG	1 cut(s)	236
SinI	GGWCC	8 cut(s)	53, 154, 220, 296, 931, 2112, 2705, 4549
SmaI	CCCGGG	1 cut(s)	4158
SmiMI	CAYNNNNRTG	6 cut(s)	1242, 1817, 1831, 2239, 2831, 3759
SmlI	CTYRAG	5 cut(s)	1454, 2129, 2966, 3308, 3925
SmoI	CTYRAG	5 cut(s)	1454, 2129, 2966, 3308, 3925
SpeI	ACTAGT	1 cut(s)	3713
SphI	GCATGC	2 cut(s)	1852, 2540
Sse8387I	CCTGCAGG	1 cut(s)	1438
StyD4I	CCNGG	7 cut(s)	933, 1843, 2058, 3871, 4155, 4156, 4203
StyI	CCWWGG	2 cut(s)	106, 250
TaiI	ACGT	6 cut(s)	461, 2455, 3748, 3844, 4262, 4369
TaqII	GACCGA	2 cut(s)	171, 2328
TatI	WGTACW	5 cut(s)	1160, 1450, 4239, 4380, 4398
TauI	GCSGC	8 cut(s)	43, 117, 324, 416, 419, 422, 1023, 2127
TseFI	GTSAC	4 cut(s)	1793, 2249, 4098, 4262
TseI	GCWGC	9 cut(s)	1312, 3301, 3326, 4026, 4356, 4405, 4443, 4523, 4576
Tsp45I	GTSAC	4 cut(s)	1793, 2249, 4098, 4262
TspGWI	ACGGA	7 cut(s)	288, 1292, 1862, 3546, 4377, 4400, 4578
TspMI	CCCGGG	1 cut(s)	4156
Tth111I	GACNNNGTC	1 cut(s)	170
Van91I	CCANNNNNTGG	1 cut(s)	4429
Vha464I	CTTAAG	2 cut(s)	1454, 3308
VpaK11BI	GGWCC	8 cut(s)	53, 154, 220, 296, 931, 2112, 2705, 4549
VspI	ATTAAT	2 cut(s)	726, 1755
XceI	RCATGY	5 cut(s)	1198, 1852, 2540, 3620, 4020
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2106
XmaI	CCCGGG	1 cut(s)	4156
XmaJI	CCTAGG	1 cut(s)	250
XmiI	GTMKAC	2 cut(s)	237, 1209
XmnI	GAANNNNTTC	1 cut(s)	3489
ZraI	GACGTC	1 cut(s)	4367
Zsp2I	ATGCAT	2 cut(s)	1414, 2404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.