DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 242 |
| AccII | CGCG | 1 cut(s) | 248 |
| AciI | CCGC | 1 cut(s) | 246 |
| AclWI | GGATC | 2 cut(s) | 131, 417 |
| AgsI | TTSAA | 2 cut(s) | 40, 86 |
| AjnI | CCWGG | 2 cut(s) | 98, 320 |
| AluBI | AGCT | 1 cut(s) | 421 |
| AluI | AGCT | 1 cut(s) | 421 |
| Alw26I | GTCTC | 4 cut(s) | 155, 287, 341, 346 |
| AlwI | GGATC | 2 cut(s) | 131, 417 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 230 |
| Ama87I | CYCGRG | 1 cut(s) | 165 |
| ApeKI | GCWGC | 3 cut(s) | 5, 152, 230 |
| AvaI | CYCGRG | 1 cut(s) | 165 |
| BaeGI | GKGCMC | 1 cut(s) | 133 |
| BanI | GGYRCC | 1 cut(s) | 242 |
| BbsI | GAAGAC | 1 cut(s) | 332 |
| BbvCI | CCTCAGC | 1 cut(s) | 360 |
| BbvI | GCAGC | 3 cut(s) | 17, 164, 217 |
| BccI | CCATC | 2 cut(s) | 271, 292 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 31 |
| BciT130I | CCWGG | 2 cut(s) | 100, 322 |
| BciVI | GTATCC | 1 cut(s) | 78 |
| BcoDI | GTCTC | 4 cut(s) | 155, 287, 341, 346 |
| BfuI | GTATCC | 1 cut(s) | 78 |
| BglII | AGATCT | 1 cut(s) | 29 |
| BisI | GCNGC | 4 cut(s) | 6, 153, 231, 246 |
| BlsI | GCNGC | 4 cut(s) | 7, 154, 232, 247 |
| Bme1390I | CCNGG | 2 cut(s) | 100, 322 |
| BmeT110I | CYCGRG | 1 cut(s) | 165 |
| BmiI | GGNNCC | 1 cut(s) | 244 |
| BmrFI | CCNGG | 2 cut(s) | 100, 322 |
| BmsI | GCATC | 1 cut(s) | 217 |
| BpiI | GAAGAC | 1 cut(s) | 332 |
| Bpu10I | CCTNAGC | 1 cut(s) | 360 |
| BsaI | GGTCTC | 2 cut(s) | 155, 346 |
| BsaJI | CCNNGG | 1 cut(s) | 99 |
| Bse1I | ACTGG | 1 cut(s) | 217 |
| BseBI | CCWGG | 2 cut(s) | 100, 322 |
| BseDI | CCNNGG | 1 cut(s) | 99 |
| BseMII | CTCAG | 1 cut(s) | 351 |
| BseNI | ACTGG | 1 cut(s) | 217 |
| BseSI | GKGCMC | 1 cut(s) | 133 |
| BseXI | GCAGC | 3 cut(s) | 17, 164, 217 |
| Bsh1236I | CGCG | 1 cut(s) | 248 |
| BshNI | GGYRCC | 1 cut(s) | 242 |
| BsiHKCI | CYCGRG | 1 cut(s) | 165 |
| BsmAI | GTCTC | 4 cut(s) | 155, 287, 341, 346 |
| BsmBI | CGTCTC | 1 cut(s) | 287 |
| Bso31I | GGTCTC | 2 cut(s) | 155, 346 |
| BsoBI | CYCGRG | 1 cut(s) | 165 |
| Bsp1286I | GDGCHC | 1 cut(s) | 133 |
| Bsp143I | GATC | 4 cut(s) | 29, 136, 409, 436 |
| BspACI | CCGC | 1 cut(s) | 246 |
| BspCNI | CTCAG | 1 cut(s) | 352 |
| BspFNI | CGCG | 1 cut(s) | 248 |
| BspHI | TCATGA | 1 cut(s) | 430 |
| BspLI | GGNNCC | 1 cut(s) | 244 |
| BspPI | GGATC | 2 cut(s) | 131, 417 |
| BspT107I | GGYRCC | 1 cut(s) | 242 |
| BspTNI | GGTCTC | 2 cut(s) | 155, 346 |
| BsrI | ACTGG | 1 cut(s) | 217 |
| BssECI | CCNNGG | 1 cut(s) | 99 |
| BssMI | GATC | 4 cut(s) | 29, 136, 409, 436 |
| Bst2UI | CCWGG | 2 cut(s) | 100, 322 |
| Bst4CI | ACNGT | 1 cut(s) | 305 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 230 |
| BstC8I | GCNNGC | 1 cut(s) | 106 |
| BstDEI | CTNAG | 2 cut(s) | 360, 417 |
| BstFNI | CGCG | 1 cut(s) | 248 |
| BstKTI | GATC | 4 cut(s) | 32, 139, 412, 439 |
| BstMAI | GTCTC | 4 cut(s) | 155, 287, 341, 346 |
| BstMBI | GATC | 4 cut(s) | 29, 136, 409, 436 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 230 |
| BstNI | CCWGG | 2 cut(s) | 100, 322 |
| BstSCI | CCNGG | 2 cut(s) | 98, 320 |
| BstSLI | GKGCMC | 1 cut(s) | 133 |
| BstUI | CGCG | 1 cut(s) | 248 |
| BstV1I | GCAGC | 3 cut(s) | 17, 164, 217 |
| BstV2I | GAAGAC | 1 cut(s) | 332 |
| BstX2I | RGATCY | 3 cut(s) | 29, 136, 409 |
| BstYI | RGATCY | 3 cut(s) | 29, 136, 409 |
| BsuI | GTATCC | 1 cut(s) | 78 |
| BtsIMutI | CAGTG | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 1 cut(s) | 106 |
| CaiI | CAGNNNCTG | 1 cut(s) | 230 |
| CciI | TCATGA | 1 cut(s) | 430 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 243, 278 |
| CviAII | CATG | 3 cut(s) | 199, 239, 431 |
| CviJI | RGCY | 2 cut(s) | 365, 421 |
| CviKI_1 | RGCY | 2 cut(s) | 365, 421 |
| DdeI | CTNAG | 2 cut(s) | 360, 417 |
| DpnI | GATC | 4 cut(s) | 31, 138, 411, 438 |
| DpnII | GATC | 4 cut(s) | 29, 136, 409, 436 |
| Eco31I | GGTCTC | 2 cut(s) | 155, 346 |
| Eco88I | CYCGRG | 1 cut(s) | 165 |
| EcoRII | CCWGG | 2 cut(s) | 98, 320 |
| Esp3I | CGTCTC | 1 cut(s) | 287 |
| FaeI | CATG | 3 cut(s) | 202, 242, 434 |
| FaiI | YATR | 6 cut(s) | 123, 200, 240, 312, 396, 432 |
| FatI | CATG | 3 cut(s) | 198, 238, 430 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 153, 231, 246 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 153, 231, 246 |
| GluI | GCNGC | 4 cut(s) | 6, 153, 231, 246 |
| Hin1II | CATG | 3 cut(s) | 202, 242, 434 |
| HindIII | AAGCTT | 1 cut(s) | 419 |
| Hpy188I | TCNGA | 1 cut(s) | 346 |
| Hpy188III | TCNNGA | 1 cut(s) | 431 |
| HpyAV | CCTTC | 1 cut(s) | 81 |
| HpyCH4III | ACNGT | 1 cut(s) | 305 |
| HpyCH4IV | ACGT | 1 cut(s) | 127 |
| HpyCH4V | TGCA | 1 cut(s) | 233 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 230 |
| HpyF3I | CTNAG | 2 cut(s) | 360, 417 |
| HpySE526I | ACGT | 1 cut(s) | 127 |
| Hsp92II | CATG | 3 cut(s) | 202, 242, 434 |
| Kzo9I | GATC | 4 cut(s) | 29, 136, 409, 436 |
| LpnPI | CCDG | 5 cut(s) | 85, 112, 198, 307, 334 |
| Lsp1109I | GCAGC | 3 cut(s) | 17, 164, 217 |
| LweI | GCATC | 1 cut(s) | 217 |
| MaeII | ACGT | 1 cut(s) | 127 |
| MaeIII | GTNAC | 2 cut(s) | 184, 208 |
| MalI | GATC | 4 cut(s) | 31, 138, 411, 438 |
| MboI | GATC | 4 cut(s) | 29, 136, 409, 436 |
| MboII | GAAGA | 1 cut(s) | 337 |
| MfeI | CAATTG | 1 cut(s) | 156 |
| MflI | RGATCY | 3 cut(s) | 29, 136, 409 |
| MhlI | GDGCHC | 1 cut(s) | 133 |
| MluCI | AATT | 2 cut(s) | 156, 316 |
| MnlI | CCTC | 6 cut(s) | 53, 253, 262, 355, 400, 427 |
| MspR9I | CCNGG | 2 cut(s) | 100, 322 |
| MunI | CAATTG | 1 cut(s) | 156 |
| MvaI | CCWGG | 2 cut(s) | 100, 322 |
| MvnI | CGCG | 1 cut(s) | 248 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 230 |
| NdeII | GATC | 4 cut(s) | 29, 136, 409, 436 |
| NlaIII | CATG | 3 cut(s) | 202, 242, 434 |
| NlaIV | GGNNCC | 1 cut(s) | 244 |
| NmuCI | GTSAC | 1 cut(s) | 184 |
| PagI | TCATGA | 1 cut(s) | 430 |
| PfoI | TCCNGGA | 1 cut(s) | 320 |
| PkrI | GCNGC | 4 cut(s) | 7, 154, 232, 247 |
| Psp6I | CCWGG | 2 cut(s) | 98, 320 |
| PspGI | CCWGG | 2 cut(s) | 98, 320 |
| PspN4I | GGNNCC | 1 cut(s) | 244 |
| PstNI | CAGNNNCTG | 1 cut(s) | 230 |
| PsuI | RGATCY | 3 cut(s) | 29, 136, 409 |
| SatI | GCNGC | 4 cut(s) | 6, 153, 231, 246 |
| Sau3AI | GATC | 4 cut(s) | 29, 136, 409, 436 |
| ScrFI | CCNGG | 2 cut(s) | 100, 322 |
| SduI | GDGCHC | 1 cut(s) | 133 |
| SetI | ASST | 5 cut(s) | 45, 130, 264, 342, 423 |
| SfaNI | GCATC | 1 cut(s) | 217 |
| Sse9I | AATT | 2 cut(s) | 156, 316 |
| SsiI | CCGC | 1 cut(s) | 246 |
| StyD4I | CCNGG | 2 cut(s) | 98, 320 |
| TaaI | ACNGT | 1 cut(s) | 305 |
| TaiI | ACGT | 1 cut(s) | 130 |
| TasI | AATT | 2 cut(s) | 156, 316 |
| TauI | GCSGC | 1 cut(s) | 248 |
| TscAI | CASTG | 1 cut(s) | 310 |
| TseFI | GTSAC | 1 cut(s) | 184 |
| TseI | GCWGC | 3 cut(s) | 5, 152, 230 |
| Tsp45I | GTSAC | 1 cut(s) | 184 |
| TspDTI | ATGAA | 1 cut(s) | 138 |
| TspRI | CASTG | 1 cut(s) | 310 |