FvH4_2g21772

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000429
Physical Location & Seq
Forward (+)
18019686 .. 18021578
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21772.t1

Sequence Viewer

Length: 978 bp
ATGGAGAAGCTCATCTCAAGCAGAAGTGATTTGCAAGATGTACCTGAATCTTTCATCTTACCCCCGGAAACTAGACCCGGAAATGCACAAGTTTCTGTAATGGAGGACATTCCTGTTATTGATTTTCAGCAACTTGGTACAGACAGACCACAGCTGATTAAGCAAATCATAGATGCTGCCCAAGAATTTGGATTCTTCCAGTTGATCAATCACGGAGTTCCAGAAAATTTGTTGCATGACATATTGGATGTGGCCAATGAGTTCTTTGAGCTGCCTTCTATAGATAAAGCAAGCTTCTGTCCTGGAGAGAAAGGGAAGGTACATAACTGGAGAGAAAAATTGAGACACCCTTGTCATCCATTAGAGGAACATCTGCAAGCCTGGCCTCAGAAACCAATTAAATACCGAGAGGTGGTTGGAAATTATTCGGTGGAGGTGAGGAAGCTGAGTTTGTGTCTTCTGGATCTGATCTGTGAAGGATTGGAACTTGAATCTGAGTTTTTCAAGAGTGAACTTAGCCAAGTGCAAATAATGGGTATAAGCTATTACCCACCATGCCCGGACCCAACATTAACCTTGGGATTACTTAAACATTGTGATGGAAATCTCATAACTGTTCTTCTGCAAGAACAAGTTCATGGCCTTCAAGTCTTCAAAGATGAGCAATGGTTGGCTGTTGAGCCTGTCCCTAATGCCTTGGTAGTTAACATAGGCCACACTTTGCAGATTATCAGTAATGGGAAGCTAAGCAGTGCAGAGCACAGAGTTGTGACAAACAGAAAGGTTTCGAGGATGTCAGTTGCGAGCTTCATCCATCCTTCTGGAAACTGCCACATTGAACCTGCAAAAGCACTTCTTGTTAAGAACTGCAGTACTCCACTCTACAGAGATTTTATATACAAAGACTTTCTTAGCACCTATATAACAGATACCCTAGAGGGATTACCACCACTTGAGCGGTATAAGCTCCAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

326

Amino Acids

36.84

Weight (kDa)

5.47

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 37 - 99 9e-19 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 176 - 273 9.2e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 351
Acc36I ACCTGC 1 cut(s) 850
AccBSI CCGCTC 1 cut(s) 958
AciI CCGC 1 cut(s) 958
AclWI GGATC 1 cut(s) 471
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 185, 226
AfaI GTAC 4 cut(s) 42, 139, 321, 874
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 5 cut(s) 491, 505, 647, 655, 839
AjnI CCWGG 2 cut(s) 301, 380
AluBI AGCT 9 cut(s) 10, 154, 271, 294, 445, 543, 745, 807, 967
AluI AGCT 9 cut(s) 10, 154, 271, 294, 445, 543, 745, 807, 967
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 1 cut(s) 471
AoxI GGCC 4 cut(s) 252, 383, 640, 712
ApeKI GCWGC 2 cut(s) 176, 271
ApoI RAATTY 2 cut(s) 185, 226
Asp700I GAANNNNTTC 3 cut(s) 424, 633, 784
AspS9I GGNCC 1 cut(s) 562
AsuC2I CCSGG 3 cut(s) 65, 78, 560
AsuHPI GGTGA 1 cut(s) 448
AvaII GGWCC 1 cut(s) 562
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 2 cut(s) 449, 643
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 2 cut(s) 163, 258
BccI CCATC 2 cut(s) 593, 822
BciT130I CCWGG 2 cut(s) 303, 382
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 3 cut(s) 65, 78, 560
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 2 cut(s) 72, 935
BfmI CTRYAG 3 cut(s) 279, 868, 883
BfuAI ACCTGC 1 cut(s) 850
BisI GCNGC 2 cut(s) 177, 272
BlpI GCTNAGC 1 cut(s) 746
BlsI GCNGC 2 cut(s) 178, 273
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 5 cut(s) 65, 78, 303, 382, 560
Bme18I GGWCC 1 cut(s) 562
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 5 cut(s) 65, 78, 303, 382, 560
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 2 cut(s) 449, 643
BpmI CTGGAG 3 cut(s) 324, 349, 953
Bpu1102I GCTNAGC 1 cut(s) 746
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 3 cut(s) 65, 78, 560
BsaBI GATNNNNATC 1 cut(s) 603
BsaJI CCNNGG 3 cut(s) 63, 576, 696
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse1I ACTGG 2 cut(s) 199, 332
Bse3DI GCAATG 1 cut(s) 671
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 2 cut(s) 303, 382
BseDI CCNNGG 3 cut(s) 63, 576, 696
BseGI GGATG 5 cut(s) 253, 355, 798, 810, 814
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 3 cut(s) 401, 437, 486
BseNI ACTGG 2 cut(s) 199, 332
BseXI GCAGC 2 cut(s) 163, 258
BsgI GTGCAG 1 cut(s) 774
BshFI GGCC 4 cut(s) 254, 385, 642, 714
BsiHKAI GWGCWC 1 cut(s) 762
BsiSI CCGG 3 cut(s) 65, 78, 560
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 671
BsnI GGCC 4 cut(s) 254, 385, 642, 714
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 3 cut(s) 204, 463, 468
Bsp1720I GCTNAGC 1 cut(s) 746
BspACI CCGC 1 cut(s) 958
BspANI GGCC 4 cut(s) 254, 385, 642, 714
BspCNI CTCAG 3 cut(s) 400, 438, 487
BspLI GGNNCC 1 cut(s) 564
BspMAI CTGCAG 1 cut(s) 872
BspMI ACCTGC 1 cut(s) 850
BspPI GGATC 1 cut(s) 471
BsrBI CCGCTC 1 cut(s) 958
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 2 cut(s) 199, 332
BssECI CCNNGG 3 cut(s) 63, 576, 696
BssMI GATC 3 cut(s) 204, 463, 468
BssT1I CCWWGG 2 cut(s) 576, 696
Bst2UI CCWGG 2 cut(s) 303, 382
Bst4CI ACNGT 1 cut(s) 616
BstC8I GCNNGC 3 cut(s) 292, 378, 805
BstDEI CTNAG 6 cut(s) 387, 446, 495, 515, 746, 911
BstF5I GGATG 5 cut(s) 253, 355, 798, 810, 814
BstKTI GATC 3 cut(s) 207, 466, 471
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 3 cut(s) 204, 463, 468
BstMWI GCNNNNNNNGC 3 cut(s) 160, 382, 964
BstNI CCWGG 2 cut(s) 303, 382
BstSCI CCNGG 5 cut(s) 63, 76, 301, 380, 558
BstSFI CTRYAG 3 cut(s) 279, 868, 883
BstV1I GCAGC 2 cut(s) 163, 258
BstV2I GAAGAC 2 cut(s) 449, 643
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 2 cut(s) 188, 821
BstYI RGATCY 1 cut(s) 463
BsuRI GGCC 4 cut(s) 254, 385, 642, 714
BtsCI GGATG 5 cut(s) 253, 355, 798, 810, 814
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 1 cut(s) 757
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 3 cut(s) 292, 378, 805
Cfr13I GGNCC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 41, 138, 320, 873
CviAII CATG 3 cut(s) 236, 555, 638
CviQI GTAC 4 cut(s) 41, 138, 320, 873
DdeI CTNAG 6 cut(s) 387, 446, 495, 515, 746, 911
DpnI GATC 3 cut(s) 206, 465, 470
DpnII GATC 3 cut(s) 204, 463, 468
DrdI GACNNNNNNGTC 1 cut(s) 351
DseDI GACNNNNNNGTC 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 576, 696
Eco47I GGWCC 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 301, 380
EcoT14I CCWWGG 2 cut(s) 576, 696
ErhI CCWWGG 2 cut(s) 576, 696
FaeI CATG 3 cut(s) 239, 558, 641
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 894, 926
FaqI GGGAC 1 cut(s) 671
FatI CATG 3 cut(s) 235, 554, 637
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 177, 272
FokI GGATG 5 cut(s) 260, 342, 797, 801, 805
Fsp4HI GCNGC 2 cut(s) 177, 272
FspBI CTAG 2 cut(s) 72, 935
GluI GCNGC 2 cut(s) 177, 272
GsuI CTGGAG 3 cut(s) 324, 349, 953
HaeIII GGCC 4 cut(s) 254, 385, 642, 714
HapII CCGG 3 cut(s) 65, 78, 560
Hin1II CATG 3 cut(s) 239, 558, 641
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 3 cut(s) 47, 192, 491
HpaI GTTAAC 1 cut(s) 706
HpaII CCGG 3 cut(s) 65, 78, 560
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 2 cut(s) 512, 706
Hpy188I TCNGA 3 cut(s) 390, 468, 496
Hpy188III TCNNGA 4 cut(s) 221, 461, 505, 822
Hpy8I GTNNAC 2 cut(s) 512, 706
HpyAV CCTTC 5 cut(s) 285, 310, 470, 653, 828
HpyCH4III ACNGT 1 cut(s) 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 382, 964
HpyF3I CTNAG 6 cut(s) 387, 446, 495, 515, 746, 911
Hsp92II CATG 3 cut(s) 239, 558, 641
Ksp22I TGATCA 1 cut(s) 204
KspAI GTTAAC 1 cut(s) 706
Kzo9I GATC 3 cut(s) 204, 463, 468
LmnI GCTCC 1 cut(s) 972
Lsp1109I GCAGC 2 cut(s) 163, 258
LweI GCATC 1 cut(s) 163
MaeI CTAG 2 cut(s) 72, 935
MaeIII GTNAC 1 cut(s) 769
MalI GATC 3 cut(s) 206, 465, 470
MbiI CCGCTC 1 cut(s) 958
MboI GATC 3 cut(s) 204, 463, 468
MboII GAAGA 4 cut(s) 187, 449, 611, 643
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 5 cut(s) 185, 226, 338, 396, 421
MluNI TGGCCA 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 8 cut(s) 97, 358, 396, 403, 427, 432, 783, 931
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 3 cut(s) 424, 633, 784
MscI TGGCCA 1 cut(s) 254
MseI TTAA 6 cut(s) 159, 399, 572, 588, 705, 861
MslI CAYNNNNRTG 1 cut(s) 597
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 3 cut(s) 65, 78, 560
MspR9I CCNGG 5 cut(s) 65, 78, 303, 382, 560
MvaI CCWGG 2 cut(s) 303, 382
MwoI GCNNNNNNNGC 3 cut(s) 160, 382, 964
NciI CCSGG 3 cut(s) 65, 78, 560
NdeII GATC 3 cut(s) 204, 463, 468
NlaIII CATG 3 cut(s) 239, 558, 641
NlaIV GGNNCC 1 cut(s) 564
NmuCI GTSAC 1 cut(s) 769
PdmI GAANNNNTTC 3 cut(s) 424, 633, 784
PfeI GAWTC 3 cut(s) 47, 192, 491
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 2 cut(s) 178, 273
Psp6I CCWGG 2 cut(s) 301, 380
PspGI CCWGG 2 cut(s) 301, 380
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 1 cut(s) 562
PstI CTGCAG 1 cut(s) 872
PsuI RGATCY 1 cut(s) 463
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 4 cut(s) 42, 139, 321, 874
RsaNI GTAC 4 cut(s) 41, 138, 320, 873
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 6 cut(s) 159, 399, 572, 588, 705, 861
SatI GCNGC 2 cut(s) 177, 272
Sau3AI GATC 3 cut(s) 204, 463, 468
Sau96I GGNCC 1 cut(s) 562
ScaI AGTACT 1 cut(s) 874
ScrFI CCNGG 5 cut(s) 65, 78, 303, 382, 560
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 1 cut(s) 163
SfcI CTRYAG 3 cut(s) 279, 868, 883
SinI GGWCC 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 2 cut(s) 16, 953
SmoI CTYRAG 2 cut(s) 16, 953
Sse9I AATT 5 cut(s) 185, 226, 338, 396, 421
SsiI CCGC 1 cut(s) 958
SspMI CTAG 2 cut(s) 72, 935
StyD4I CCNGG 5 cut(s) 63, 76, 301, 380, 558
StyI CCWWGG 2 cut(s) 576, 696
TaaI ACNGT 1 cut(s) 616
TaqI TCGA 1 cut(s) 788
TasI AATT 5 cut(s) 185, 226, 338, 396, 421
TatI WGTACW 1 cut(s) 872
TfiI GAWTC 3 cut(s) 47, 192, 491
Tru1I TTAA 6 cut(s) 159, 399, 572, 588, 705, 861
Tru9I TTAA 6 cut(s) 159, 399, 572, 588, 705, 861
TscAI CASTG 1 cut(s) 757
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 2 cut(s) 176, 271
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 3 cut(s) 43, 626, 799
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 1 cut(s) 757
VpaK11BI GGWCC 1 cut(s) 562
XapI RAATTY 2 cut(s) 185, 226
XmnI GAANNNNTTC 3 cut(s) 424, 633, 784
XspI CTAG 2 cut(s) 72, 935
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.