Rw6G026520

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000429
Physical Location & Seq
Forward (+)
49916607 .. 49918289
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026520.1

Sequence Viewer

Length: 1035 bp
ATGGAGAAGCTTGTTCCAAACTGGTGCAGTGCTGCTCAACCCTTGCCTGAGACATACATATTCCCACCTGATGCAAGACCAGGGAAGTTTAGCGTTGCTCCTTCAATATCCAACGACATTCCAGTGATTGATCTAGGATCACAGAGTCATGATCGAGCTCTTGTGATCCAGCAAATACTTGAGGCTAGCCAAGAATTCGGTTTCTTTCAGGTAATCAACCATGGCATACCAGAAGCTTTAATGAATGACACAATGGATGTGTTCAAGGAGTTCTTTGAGTTGCCTCTAGAGGAAAAGAGGGCAAGCATCTACTCTGATGACCCCAACAAAGTCTGCAAGCTCGTACATAGCAGTGTTAATTACGATTGGGAGGAAGTTCATCTTTGGCGCGATTTCCTCCGACACCCTTGTGAACCTTTAGAAAAATTAATGCCAATTTGGCCTCGGAAACCGATTAAATATCGAGAGCATGTTAGCAAATGTTTCACCCAAGTGAAGAAAGTGGCTTTGGACATTTTGGAGTTGATCGGTGAAGGACTTGGGATAGGATCAGAGTATTTCAATGATGAACTTAGCAAAGAGACTGACATCTTTGTTAATCACTATCCACCTTGTCCTGACCCAAGTTTGACACTTGGAATAACTAAACACTCTGACCCACAGCTCATCACAATTTTACTTCAAGGGGATGTCAGTGGCCTTCAAGTTCTCAATAAGGATGGCGAATGGATTGGAGTGGAACCTATTTCAAATGGACTGGTGGTTAACATAGGCTATCAATTACGGATCATTAGTAATGGAAAGCTCAAGTGTGCTGAACATCGAGTGGTGACGAATTCAAGTACTGTTCGGACGACGATTGGATTTTTCATTACACCCTCCCCTGACTGCTATATAGAACCTGCAGCAGCTCTTATTAATGCAAGCAATCCCCGACTCTATAAAGGCTTCCGATATGAAGAGTTTCGTGCCAACTACTTTAGGAAGCAGGGGAAAACCGAAGTTGTGCTTGAACCCTTCAAAATCGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

344

Amino Acids

39.03

Weight (kDa)

5.37

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 40 - 147 3e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 197 - 293 5e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 910
AccII CGCG 1 cut(s) 390
AclWI GGATC 4 cut(s) 145, 160, 556, 794
AcsI RAATTY 2 cut(s) 194, 835
AfaI GTAC 2 cut(s) 345, 844
AgsI TTSAA 9 cut(s) 105, 265, 562, 683, 704, 750, 840, 1013, 1021
AjnI CCWGG 1 cut(s) 79
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AleI CACNNNNGTG 2 cut(s) 408, 491
AluBI AGCT 7 cut(s) 10, 158, 236, 340, 664, 805, 911
AluI AGCT 7 cut(s) 10, 158, 236, 340, 664, 805, 911
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 44, 575
AlwI GGATC 4 cut(s) 145, 160, 556, 794
AoxI GGCC 2 cut(s) 440, 697
ApeKI GCWGC 3 cut(s) 32, 905, 908
ApoI RAATTY 2 cut(s) 194, 835
AseI ATTAAT 2 cut(s) 428, 918
Asp700I GAANNNNTTC 1 cut(s) 963
AspLEI GCGC 1 cut(s) 390
AsuHPI GGTGA 3 cut(s) 478, 542, 841
AsuNHI GCTAGC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 19, 917, 920
BccI CCATC 1 cut(s) 713
BciT130I CCWGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 44, 575
BfaI CTAG 3 cut(s) 134, 186, 287
BfmI CTRYAG 1 cut(s) 903
BfuAI ACCTGC 1 cut(s) 910
BglI GCCNNNNNGGC 1 cut(s) 439
BisI GCNGC 3 cut(s) 33, 906, 909
BlsI GCNGC 3 cut(s) 34, 907, 910
BmcAI AGTACT 1 cut(s) 844
Bme1390I CCNGG 1 cut(s) 81
BmiI GGNNCC 1 cut(s) 741
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 61, 315
BmtI GCTAGC 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 200, 791
BsaJI CCNNGG 3 cut(s) 80, 220, 443
Bse1I ACTGG 3 cut(s) 26, 122, 762
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 80, 220, 443
BseGI GGATG 3 cut(s) 262, 694, 724
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 3 cut(s) 26, 122, 762
BseXI GCAGC 3 cut(s) 19, 917, 920
BsgI GTGCAG 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 2 cut(s) 442, 699
BsiHKAI GWGCWC 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 44, 575
BsnI GGCC 2 cut(s) 442, 699
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
Bsp19I CCATGG 1 cut(s) 220
BspANI GGCC 2 cut(s) 442, 699
BspCNI CTCAG 1 cut(s) 40
BspFNI CGCG 1 cut(s) 390
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 741
BspMAI CTGCAG 1 cut(s) 907
BspMI ACCTGC 1 cut(s) 910
BspOI GCTAGC 1 cut(s) 189
BspPI GGATC 4 cut(s) 145, 160, 556, 794
BsrI ACTGG 3 cut(s) 26, 122, 762
BssECI CCNNGG 3 cut(s) 80, 220, 443
BssMI GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 847
Bst6I CTCTTC 1 cut(s) 954
BstC8I GCNNGC 4 cut(s) 187, 304, 338, 925
BstDEI CTNAG 3 cut(s) 48, 572, 1032
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 3 cut(s) 262, 694, 724
BstFNI CGCG 1 cut(s) 390
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 7 cut(s) 133, 140, 154, 168, 528, 551, 789
BstMAI GTCTC 2 cut(s) 44, 575
BstMBI GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
BstMWI GCNNNNNNNGC 1 cut(s) 439
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 903
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 3 cut(s) 19, 917, 920
BsuRI GGCC 2 cut(s) 442, 699
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 262, 694, 724
BtsI GCAGTG 2 cut(s) 34, 358
BtsIMutI CAGTG 4 cut(s) 34, 129, 358, 700
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 4 cut(s) 187, 304, 338, 925
CciI TCATGA 1 cut(s) 148
CfoI GCGC 1 cut(s) 390
Csp6I GTAC 2 cut(s) 344, 843
CviAII CATG 3 cut(s) 149, 221, 470
CviQI GTAC 2 cut(s) 344, 843
DdeI CTNAG 3 cut(s) 48, 572, 1032
DpnI GATC 7 cut(s) 132, 139, 153, 167, 527, 550, 788
DpnII GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
Eam1104I CTCTTC 1 cut(s) 954
EarI CTCTTC 1 cut(s) 954
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 158
EcoRI GAATTC 2 cut(s) 194, 835
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 3 cut(s) 152, 224, 473
FalI AAGNNNNNCTT 6 cut(s) 257, 289, 366, 398, 993, 1025
FatI CATG 3 cut(s) 148, 220, 469
Fnu4HI GCNGC 3 cut(s) 33, 906, 909
FokI GGATG 3 cut(s) 269, 701, 731
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 3 cut(s) 33, 906, 909
FspBI CTAG 3 cut(s) 134, 186, 287
GlaI GCGC 1 cut(s) 389
GluI GCNGC 3 cut(s) 33, 906, 909
HaeIII GGCC 2 cut(s) 442, 699
HhaI GCGC 1 cut(s) 390
Hin1II CATG 3 cut(s) 152, 224, 473
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 2 cut(s) 8, 234
HinfI GANTC 3 cut(s) 145, 936, 1028
HpaI GTTAAC 1 cut(s) 766
HphI GGTGA 3 cut(s) 478, 542, 841
Hpy166II GTNNAC 2 cut(s) 413, 766
Hpy188I TCNGA 7 cut(s) 316, 401, 447, 553, 655, 852, 953
Hpy188III TCNNGA 4 cut(s) 149, 287, 464, 617
Hpy8I GTNNAC 2 cut(s) 413, 766
Hpy99I CGWCG 1 cut(s) 859
HpyAV CCTTC 4 cut(s) 111, 527, 710, 1027
HpyCH4III ACNGT 1 cut(s) 847
HpyCH4V TGCA 5 cut(s) 27, 74, 336, 905, 923
HpyF10VI GCNNNNNNNGC 1 cut(s) 439
HpyF3I CTNAG 3 cut(s) 48, 572, 1032
Hsp92II CATG 3 cut(s) 152, 224, 473
HspAI GCGC 1 cut(s) 388
KspAI GTTAAC 1 cut(s) 766
Kzo9I GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 3 cut(s) 19, 917, 920
LweI GCATC 2 cut(s) 61, 315
MaeI CTAG 3 cut(s) 134, 186, 287
MaeIII GTNAC 1 cut(s) 829
MalI GATC 7 cut(s) 132, 139, 153, 167, 527, 550, 788
MboI GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
MboII GAAGA 2 cut(s) 508, 971
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 7 cut(s) 194, 358, 425, 435, 672, 779, 835
MlyI GAGTC 2 cut(s) 154, 930
MmeI TCCRAC 2 cut(s) 135, 424
MnlI CCTC 8 cut(s) 175, 283, 291, 294, 364, 407, 453, 889
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 7 cut(s) 239, 357, 428, 456, 597, 765, 918
MslI CAYNNNNRTG 4 cut(s) 122, 351, 408, 491
MspR9I CCNGG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 439
NcoI CCATGG 1 cut(s) 220
NdeII GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
NheI GCTAGC 1 cut(s) 185
NlaIII CATG 3 cut(s) 152, 224, 473
NlaIV GGNNCC 1 cut(s) 741
NmuCI GTSAC 1 cut(s) 829
NspI RCATGY 1 cut(s) 473
OliI CACNNNNGTG 2 cut(s) 408, 491
PagI TCATGA 1 cut(s) 148
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 1 cut(s) 1028
PkrI GCNGC 3 cut(s) 34, 907, 910
PleI GAGTC 2 cut(s) 153, 930
PpsI GAGTC 2 cut(s) 153, 930
PshBI ATTAAT 2 cut(s) 428, 918
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 741
PstI CTGCAG 1 cut(s) 907
RsaI GTAC 2 cut(s) 345, 844
RsaNI GTAC 2 cut(s) 344, 843
RseI CAYNNNNRTG 4 cut(s) 122, 351, 408, 491
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 7 cut(s) 239, 357, 428, 456, 597, 765, 918
SatI GCNGC 3 cut(s) 33, 906, 909
Sau3AI GATC 7 cut(s) 130, 137, 151, 165, 525, 548, 786
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 2 cut(s) 154, 930
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 2 cut(s) 61, 315
SfcI CTRYAG 1 cut(s) 903
SmiMI CAYNNNNRTG 4 cut(s) 122, 351, 408, 491
SmlI CTYRAG 2 cut(s) 179, 806
SmoI CTYRAG 2 cut(s) 179, 806
Sse9I AATT 7 cut(s) 194, 358, 425, 435, 672, 779, 835
SspMI CTAG 3 cut(s) 134, 186, 287
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 847
TaqI TCGA 4 cut(s) 154, 463, 823, 1026
TasI AATT 7 cut(s) 194, 358, 425, 435, 672, 779, 835
TatI WGTACW 1 cut(s) 842
TfiI GAWTC 1 cut(s) 1028
Tru1I TTAA 7 cut(s) 239, 357, 428, 456, 597, 765, 918
Tru9I TTAA 7 cut(s) 239, 357, 428, 456, 597, 765, 918
TscAI CASTG 4 cut(s) 34, 129, 358, 700
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 3 cut(s) 32, 905, 908
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 5 cut(s) 257, 368, 582, 859, 972
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 4 cut(s) 34, 129, 358, 700
VspI ATTAAT 2 cut(s) 428, 918
XapI RAATTY 2 cut(s) 194, 835
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 3 cut(s) 134, 186, 287
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.