RchiOBHm_Chr6g0288471

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000429
Physical Location & Seq
Forward (+)
51728554 .. 51729523
970 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25883

Sequence Viewer

Length: 588 bp
ATGCAGTTGATCAATCATGGGATTAAAGATAACTTGTTGGATAAGGTATTGAATGTAGCCAATGAGTTCTTCGAGCTGCCTAGTGACGACAAAGCAAGTGTCTATTCCGGAAATTCTATGCAAAGCTATAGATTACACACCAGCATCGACTATATGAGAGAACAGGTACATTACTGGAGAGACGTTTTGCGACACCCTTGTCATCCTTTAGAGGAGCACATCCGATTCTGGCCACAGAAACCACCTCAATATCGAGATTTGGTTGGAAGTTACTCTGTGGAAGTGAGGAAGCTGGGTTTGTGTCTTTTGGATCTAATTTCTGAAGGATTAGGACTTGGATCTGGCTTTTTTGGGGGTGAACTTAGCCAAGTGCAAATAATGGCTACAAATCATTACCCACTATGCCCAAATCCAAGCTTAACCTTGGGATTACCTAAACATAGTGATGTCAACCTCATCACTCTTCTTATACAAGGAGAAGTTTCTGGACTTCAGGTTTTGAAAGATGGGCAATTGTTAACTATTCAGCCTGTCCCTCATGCATTTCTAGTTAATATAGGCCACATCTTACAGATTGTGATCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

195

Amino Acids

21.99

Weight (kDa)

6.02

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 2 - 79 1.1e-11 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 125 - 192 1.1e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 107
AclWI GGATC 3 cut(s) 318, 346, 574
AcoI YGGCCR 1 cut(s) 230
AcsI RAATTY 1 cut(s) 112
AcuI CTGAAG 2 cut(s) 342, 476
AfaI GTAC 1 cut(s) 168
AgsI TTSAA 2 cut(s) 52, 502
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
AluBI AGCT 4 cut(s) 76, 126, 292, 417
AluI AGCT 4 cut(s) 76, 126, 292, 417
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 318, 346, 574
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 2 cut(s) 230, 559
ApeKI GCWGC 1 cut(s) 76
ApoI RAATTY 1 cut(s) 112
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AsuHPI GGTGA 1 cut(s) 368
BalI TGGCCA 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 63
BccI CCATC 1 cut(s) 500
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 81, 548
BfmI CTRYAG 1 cut(s) 127
BisI GCNGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 78
BmsI GCATC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 196
BsaBI GATNNNNATC 1 cut(s) 578
BsaJI CCNNGG 1 cut(s) 423
BsaWI WCCGGW 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 179
Bse8I GATNNNNATC 1 cut(s) 578
BseAI TCCGGA 1 cut(s) 107
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 2 cut(s) 202, 219
BseJI GATNNNNATC 1 cut(s) 578
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 2 cut(s) 232, 561
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 518
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 2 cut(s) 232, 561
Bsp1286I GDGCHC 1 cut(s) 219
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 4 cut(s) 9, 310, 338, 579
BspANI GGCC 2 cut(s) 232, 561
BspEI TCCGGA 1 cut(s) 107
BspPI GGATC 3 cut(s) 318, 346, 574
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 4 cut(s) 9, 310, 338, 579
BssT1I CCWWGG 1 cut(s) 423
Bst6I CTCTTC 1 cut(s) 468
BstDEI CTNAG 1 cut(s) 362
BstF5I GGATG 2 cut(s) 202, 219
BstKTI GATC 4 cut(s) 12, 313, 341, 582
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 4 cut(s) 9, 310, 338, 579
BstSFI CTRYAG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 310, 338
BstYI RGATCY 2 cut(s) 310, 338
BsuRI GGCC 2 cut(s) 232, 561
BtsCI GGATG 2 cut(s) 202, 219
Csp6I GTAC 1 cut(s) 167
CviAII CATG 2 cut(s) 17, 539
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 1 cut(s) 362
DpnI GATC 4 cut(s) 11, 312, 340, 581
DpnII GATC 4 cut(s) 9, 310, 338, 579
DrdI GACNNNNNNGTC 1 cut(s) 198
DseDI GACNNNNNNGTC 1 cut(s) 198
EaeI YGGCCR 1 cut(s) 230
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 423
Eco57I CTGAAG 2 cut(s) 342, 476
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 423
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 2 cut(s) 20, 542
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 16, 538
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 1 cut(s) 77
FokI GGATG 2 cut(s) 189, 206
Fsp4HI GCNGC 1 cut(s) 77
FspBI CTAG 2 cut(s) 81, 548
GluI GCNGC 1 cut(s) 77
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 2 cut(s) 232, 561
HapII CCGG 1 cut(s) 108
Hin1II CATG 2 cut(s) 20, 542
HincII GTYRAC 2 cut(s) 451, 519
HindII GTYRAC 2 cut(s) 451, 519
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 1 cut(s) 225
HpaI GTTAAC 1 cut(s) 519
HpaII CCGG 1 cut(s) 108
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 359, 451, 519
Hpy188I TCNGA 2 cut(s) 224, 322
Hpy188III TCNNGA 3 cut(s) 108, 254, 486
Hpy8I GTNNAC 3 cut(s) 359, 451, 519
HpyAV CCTTC 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 4 cut(s) 4, 121, 373, 542
HpyF3I CTNAG 1 cut(s) 362
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 2 cut(s) 20, 542
Kpn2I TCCGGA 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 9
KspAI GTTAAC 1 cut(s) 519
Kzo9I GATC 4 cut(s) 9, 310, 338, 579
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 1 cut(s) 153
MaeI CTAG 2 cut(s) 81, 548
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 83, 269
MalI GATC 4 cut(s) 11, 312, 340, 581
MboI GATC 4 cut(s) 9, 310, 338, 579
MboII GAAGA 2 cut(s) 61, 455
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 2 cut(s) 310, 338
MhlI GDGCHC 1 cut(s) 219
MlsI TGGCCA 1 cut(s) 232
MluCI AATT 3 cut(s) 112, 315, 512
MluNI TGGCCA 1 cut(s) 232
MmeI TCCRAC 2 cut(s) 18, 244
MnlI CCTC 5 cut(s) 205, 255, 279, 464, 546
Mox20I TGGCCA 1 cut(s) 232
Mph1103I ATGCAT 1 cut(s) 544
MroI TCCGGA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 232
MseI TTAA 5 cut(s) 24, 419, 518, 552, 586
MslI CAYNNNNRTG 1 cut(s) 444
Msp20I TGGCCA 1 cut(s) 232
MspI CCGG 1 cut(s) 108
MunI CAATTG 1 cut(s) 512
NdeII GATC 4 cut(s) 9, 310, 338, 579
NlaIII CATG 2 cut(s) 20, 542
NmuCI GTSAC 1 cut(s) 83
NsiI ATGCAT 1 cut(s) 544
PfeI GAWTC 1 cut(s) 225
PkrI GCNGC 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 292
PsuI RGATCY 2 cut(s) 310, 338
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 5 cut(s) 24, 419, 518, 552, 586
SatI GCNGC 1 cut(s) 77
Sau3AI GATC 4 cut(s) 9, 310, 338, 579
SduI GDGCHC 1 cut(s) 219
SfaNI GCATC 1 cut(s) 153
SfcI CTRYAG 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 444
Sse9I AATT 3 cut(s) 112, 315, 512
SspMI CTAG 2 cut(s) 81, 548
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 1 cut(s) 186
TaqI TCGA 3 cut(s) 72, 147, 253
TasI AATT 3 cut(s) 112, 315, 512
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 5 cut(s) 24, 419, 518, 552, 586
Tru9I TTAA 5 cut(s) 24, 419, 518, 552, 586
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 83
XapI RAATTY 1 cut(s) 112
XspI CTAG 2 cut(s) 81, 548
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.