RchiOBHm_Chr6g0288521

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000429
Physical Location & Seq
Forward (+)
51757619 .. 51760071
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25888

Sequence Viewer

Length: 966 bp
ATGTTAGTTTCACCCTTTCACCTCATCTCGAACAGAAGTGACTTGCAATTTGTACCTGAATCCTACATATTACCCCCAGGAACTAGACCAGGCAATACACAGGTCTCTGTATTGGAGGTCATTCCAGTCATTGATTTTCAGCTACTTGGTACCAACAGACCTCAGCTGATCAAGCAAATCATAGAGGCTTCCCAAAAATTTGGCTTCTTCCAGGTTTATAATATACTGAATTTCGGGGAAATTCTATGGTACCTATATGTATATAATACACTCATGTACAGATCTGACAACAATTTTGTTGTCATTGTGGTAAATAGAGTTATTTTTTGGGTAATTTTAGTTCTATTACAGTTGATCAATCACGGGGTTGAAGACAATTTGCTGCATAAGGTATTGGACGTGGCCAACGAGTTCTTTGAGCAGCCTTATGAAGACAAGGCAAGCATGTATTCTGAAGATGTTGAGCAAAGTTGTAGACTATACACCAGCATTGATTATAACAAAGAGAAGGTGCATTTTTGGAGAGACAATTTGAGACACCCTTGTCATCCCTTAGAACAACACATCCATTTCTGGCCTCAGAAACCGCCTCAATATCGGGAGGTGGTTGGAAGTTATTCAGTGGAGGCAAGGAAGCTGAGTATATTGCTTTTGGATCTTATTGGAGAAGGGTTGGGACTTGAATCTGAATTTTTCGGAGATGAACTTACCGAAGTCCAACTGATGGGCATAAACTATTACCCACCTTGTCCAGACCCGAGCTTAACCTTAGGGTTACCAAAACATAGTGATGTTAACCTCATCACTCTTCTTCTCCAAGGAGAGGAAGTTCATGGTCTTCAGGTTTTAAAAGATGGGCAATGGTTGGCTGTTGAGCCTGTCCCTAATGCCTTTGTGGTTAATATAGGCCACACTTTACAGGTGCATCCACATCTTTATAACCCAGCCAGCTTCTCTTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

321

Amino Acids

37.05

Weight (kDa)

5.15

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 116 - 194 5.6e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 240 - 314 3.1e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 219, 498, 939
AasI GACNNNNNNGTC 1 cut(s) 543
Acc65I GGTACC 2 cut(s) 149, 249
AccB1I GGYRCC 2 cut(s) 149, 249
AccB7I CCANNNNNTGG 1 cut(s) 724
AccI GTMKAC 1 cut(s) 475
AciI CCGC 1 cut(s) 587
AclWI GGATC 1 cut(s) 663
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 4 cut(s) 197, 229, 240, 689
AcuI CTGAAG 2 cut(s) 474, 824
AfaI GTAC 4 cut(s) 54, 151, 251, 278
AfiI CCNNNNNNNGG 2 cut(s) 724, 823
AgsI TTSAA 2 cut(s) 371, 683
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 3 cut(s) 76, 88, 210
AloI GAACNNNNNNTCC 4 cut(s) 549, 581, 813, 845
AluBI AGCT 5 cut(s) 142, 166, 637, 762, 951
AluI AGCT 5 cut(s) 142, 166, 637, 762, 951
Alw26I GTCTC 3 cut(s) 109, 519, 529
AlwI GGATC 1 cut(s) 663
Ama87I CYCGRG 1 cut(s) 757
AoxI GGCC 3 cut(s) 402, 575, 907
ApeKI GCWGC 2 cut(s) 382, 421
ApoI RAATTY 4 cut(s) 197, 229, 240, 689
ArsI GACNNNNNNTTYG 4 cut(s) 32, 64, 278, 310
Asp700I GAANNNNTTC 1 cut(s) 616
Asp718I GGTACC 2 cut(s) 149, 249
AsuHPI GGTGA 2 cut(s) 3, 11
AvaI CYCGRG 1 cut(s) 757
AxyI CCTNAGG 1 cut(s) 769
BalI TGGCCA 1 cut(s) 404
BanI GGYRCC 2 cut(s) 149, 249
BbsI GAAGAC 3 cut(s) 378, 438, 830
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 369, 433
BccI CCATC 2 cut(s) 718, 848
BciT130I CCWGG 3 cut(s) 78, 90, 212
BclI TGATCA 2 cut(s) 168, 354
BcoDI GTCTC 3 cut(s) 109, 519, 529
BfaI CTAG 1 cut(s) 84
BglII AGATCT 1 cut(s) 281
BisI GCNGC 2 cut(s) 383, 422
BlsI GCNGC 2 cut(s) 384, 423
Bme1390I CCNGG 3 cut(s) 78, 90, 212
BmeT110I CYCGRG 1 cut(s) 757
BmgBI CACGTC 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 151, 251
BmrFI CCNGG 3 cut(s) 78, 90, 212
BmsI GCATC 1 cut(s) 934
BpiI GAAGAC 3 cut(s) 378, 438, 830
Bpu10I CCTNAGC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 76, 817
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 2 cut(s) 724, 823
Bse1I ACTGG 1 cut(s) 125
Bse21I CCTNAGG 1 cut(s) 769
Bse3DI GCAATG 1 cut(s) 866
BseBI CCWGG 3 cut(s) 78, 90, 212
BseDI CCNNGG 2 cut(s) 76, 817
BseGI GGATG 3 cut(s) 547, 564, 925
BseLI CCNNNNNNNGG 2 cut(s) 724, 823
BseMI GCAATG 1 cut(s) 866
BseMII CTCAG 3 cut(s) 176, 593, 629
BseNI ACTGG 1 cut(s) 125
BseXI GCAGC 2 cut(s) 369, 433
BseYI CCCAGC 1 cut(s) 943
BshFI GGCC 3 cut(s) 404, 577, 909
BshNI GGYRCC 2 cut(s) 149, 249
BsiHKCI CYCGRG 1 cut(s) 757
BslFI GGGAC 2 cut(s) 690, 866
BslI CCNNNNNNNGG 2 cut(s) 724, 823
BsmAI GTCTC 3 cut(s) 109, 519, 529
BsmFI GGGAC 2 cut(s) 690, 866
BsnI GGCC 3 cut(s) 404, 577, 909
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 757
Bsp1407I TGTACA 1 cut(s) 276
Bsp143I GATC 4 cut(s) 168, 281, 354, 655
BspACI CCGC 1 cut(s) 587
BspANI GGCC 3 cut(s) 404, 577, 909
BspCNI CTCAG 3 cut(s) 175, 592, 630
BspLI GGNNCC 2 cut(s) 151, 251
BspPI GGATC 1 cut(s) 663
BspT107I GGYRCC 2 cut(s) 149, 249
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 866
BsrGI TGTACA 1 cut(s) 276
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 76, 817
BssMI GATC 4 cut(s) 168, 281, 354, 655
BssT1I CCWWGG 1 cut(s) 817
Bst2UI CCWGG 3 cut(s) 78, 90, 212
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 813
BstAUI TGTACA 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 442, 949
BstDEI CTNAG 5 cut(s) 162, 553, 579, 638, 769
BstEII GGTNACC 1 cut(s) 774
BstF5I GGATG 3 cut(s) 547, 564, 925
BstKTI GATC 4 cut(s) 171, 284, 357, 658
BstMAI GTCTC 3 cut(s) 109, 519, 529
BstMBI GATC 4 cut(s) 168, 281, 354, 655
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 3 cut(s) 78, 90, 212
BstNSI RCATGY 1 cut(s) 448
BstPI GGTNACC 1 cut(s) 774
BstSCI CCNGG 3 cut(s) 76, 88, 210
BstV1I GCAGC 2 cut(s) 369, 433
BstV2I GAAGAC 3 cut(s) 378, 438, 830
BstX2I RGATCY 2 cut(s) 281, 655
BstXI CCANNNNNNTGG 1 cut(s) 200
BstYI RGATCY 2 cut(s) 281, 655
Bsu36I CCTNAGG 1 cut(s) 769
BsuRI GGCC 3 cut(s) 404, 577, 909
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 3 cut(s) 547, 564, 925
BtsIMutI CAGTG 1 cut(s) 627
Cac8I GCNNGC 2 cut(s) 442, 949
Csp6I GTAC 4 cut(s) 53, 150, 250, 277
CviAII CATG 3 cut(s) 274, 445, 833
CviQI GTAC 4 cut(s) 53, 150, 250, 277
DdeI CTNAG 5 cut(s) 162, 553, 579, 638, 769
DpnI GATC 4 cut(s) 170, 283, 356, 657
DpnII GATC 4 cut(s) 168, 281, 354, 655
DraI TTTAAA 1 cut(s) 849
DrdI GACNNNNNNGTC 1 cut(s) 543
DseDI GACNNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
Eco130I CCWWGG 1 cut(s) 817
Eco31I GGTCTC 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 474, 824
Eco81I CCTNAGG 1 cut(s) 769
Eco88I CYCGRG 1 cut(s) 757
Eco91I GGTNACC 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 774
EcoRII CCWGG 3 cut(s) 76, 88, 210
EcoT14I CCWWGG 1 cut(s) 817
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 3 cut(s) 277, 448, 836
FaqI GGGAC 2 cut(s) 690, 866
FatI CATG 3 cut(s) 273, 444, 832
FbaI TGATCA 2 cut(s) 168, 354
FblI GTMKAC 1 cut(s) 475
Fnu4HI GCNGC 2 cut(s) 383, 422
FokI GGATG 3 cut(s) 534, 551, 912
Fsp4HI GCNGC 2 cut(s) 383, 422
FspBI CTAG 1 cut(s) 84
GluI GCNGC 2 cut(s) 383, 422
GsaI CCCAGC 1 cut(s) 947
HaeIII GGCC 3 cut(s) 404, 577, 909
Hin1II CATG 3 cut(s) 277, 448, 836
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 2 cut(s) 59, 683
HpaI GTTAAC 1 cut(s) 796
HphI GGTGA 2 cut(s) 3, 11
Hpy166II GTNNAC 2 cut(s) 476, 796
Hpy188I TCNGA 5 cut(s) 286, 454, 582, 688, 698
Hpy188III TCNNGA 3 cut(s) 28, 599, 752
Hpy8I GTNNAC 2 cut(s) 476, 796
HpyAV CCTTC 2 cut(s) 502, 662
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 4 cut(s) 46, 385, 514, 925
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 5 cut(s) 162, 553, 579, 638, 769
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 3 cut(s) 277, 448, 836
KpnI GGTACC 2 cut(s) 153, 253
Ksp22I TGATCA 2 cut(s) 168, 354
KspAI GTTAAC 1 cut(s) 796
Kzo9I GATC 4 cut(s) 168, 281, 354, 655
Lsp1109I GCAGC 2 cut(s) 369, 433
LweI GCATC 1 cut(s) 934
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 2 cut(s) 38, 774
MalI GATC 4 cut(s) 170, 283, 356, 657
MboI GATC 4 cut(s) 168, 281, 354, 655
MboII GAAGA 7 cut(s) 199, 383, 443, 467, 800, 803, 830
MflI RGATCY 2 cut(s) 281, 655
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 9 cut(s) 47, 197, 229, 240, 292, 333, 376, 529, 689
MluNI TGGCCA 1 cut(s) 404
MmeI TCCRAC 2 cut(s) 589, 742
Mox20I TGGCCA 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 616
MscI TGGCCA 1 cut(s) 404
MseI TTAA 4 cut(s) 764, 795, 848, 900
MslI CAYNNNNRTG 1 cut(s) 789
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 166
MspR9I CCNGG 3 cut(s) 78, 90, 212
MvaI CCWGG 3 cut(s) 78, 90, 212
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 4 cut(s) 168, 281, 354, 655
NlaIII CATG 3 cut(s) 277, 448, 836
NlaIV GGNNCC 2 cut(s) 151, 251
NmuCI GTSAC 1 cut(s) 38
NspI RCATGY 1 cut(s) 448
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 2 cut(s) 59, 683
PflMI CCANNNNNTGG 1 cut(s) 724
PkrI GCNGC 2 cut(s) 384, 423
PsiI TTATAA 3 cut(s) 219, 498, 939
Psp6I CCWGG 3 cut(s) 76, 88, 210
PspEI GGTNACC 1 cut(s) 774
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 3 cut(s) 76, 88, 210
PspN4I GGNNCC 2 cut(s) 151, 251
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PsuI RGATCY 2 cut(s) 281, 655
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 4 cut(s) 54, 151, 251, 278
RsaNI GTAC 4 cut(s) 53, 150, 250, 277
RseI CAYNNNNRTG 1 cut(s) 789
SaqAI TTAA 4 cut(s) 764, 795, 848, 900
SatI GCNGC 2 cut(s) 383, 422
Sau3AI GATC 4 cut(s) 168, 281, 354, 655
ScrFI CCNGG 3 cut(s) 78, 90, 212
SfaNI GCATC 1 cut(s) 934
SmiMI CAYNNNNRTG 1 cut(s) 789
Sse9I AATT 9 cut(s) 47, 197, 229, 240, 292, 333, 376, 529, 689
SsiI CCGC 1 cut(s) 587
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 76, 88, 210
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 402
TaqI TCGA 1 cut(s) 29
TasI AATT 9 cut(s) 47, 197, 229, 240, 292, 333, 376, 529, 689
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 2 cut(s) 59, 683
Tru1I TTAA 4 cut(s) 764, 795, 848, 900
Tru9I TTAA 4 cut(s) 764, 795, 848, 900
TscAI CASTG 1 cut(s) 627
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 2 cut(s) 382, 421
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 3 cut(s) 444, 717, 821
TspRI CASTG 1 cut(s) 627
Van91I CCANNNNNTGG 1 cut(s) 724
XapI RAATTY 4 cut(s) 197, 229, 240, 689
XceI RCATGY 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 616
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.