Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 893 |
| AccIII | TCCGGA | 1 cut(s) | 1122 |
| AciI | CCGC | 1 cut(s) | 1314 |
| AclWI | GGATC | 1 cut(s) | 61 |
| AcsI | RAATTY | 1 cut(s) | 740 |
| AcuI | CTGAAG | 4 cut(s) | 827, 899, 960, 1284 |
| AdeI | CACNNNGTG | 1 cut(s) | 1049 |
| AfaI | GTAC | 6 cut(s) | 87, 167, 195, 353, 1297, 1360 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 641, 893, 980, 1155, 1257 |
| AgsI | TTSAA | 8 cut(s) | 179, 280, 307, 479, 649, 782, 940, 1019 |
| AjnI | CCWGG | 3 cut(s) | 635, 943, 1264 |
| AleI | CACNNNNGTG | 1 cut(s) | 558 |
| Alw26I | GTCTC | 2 cut(s) | 805, 1231 |
| AlwI | GGATC | 1 cut(s) | 61 |
| Aor13HI | TCCGGA | 1 cut(s) | 1122 |
| AoxI | GGCC | 5 cut(s) | 256, 451, 971, 1157, 1178 |
| ApeKI | GCWGC | 1 cut(s) | 1143 |
| ApoI | RAATTY | 1 cut(s) | 740 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 845, 877, 975, 1007 |
| AseI | ATTAAT | 2 cut(s) | 81, 335 |
| AspLEI | GCGC | 1 cut(s) | 1084 |
| AspS9I | GGNCC | 3 cut(s) | 257, 823, 947 |
| AsuHPI | GGTGA | 5 cut(s) | 236, 368, 545, 1061, 1205 |
| AvaII | GGWCC | 2 cut(s) | 823, 947 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 177, 210, 866, 899 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1288, 1320 |
| BbsI | GAAGAC | 3 cut(s) | 134, 276, 314 |
| BbvI | GCAGC | 1 cut(s) | 1155 |
| BccI | CCATC | 3 cut(s) | 207, 742, 1093 |
| BceAI | ACGGC | 1 cut(s) | 543 |
| BciT130I | CCWGG | 3 cut(s) | 637, 945, 1266 |
| BciVI | GTATCC | 1 cut(s) | 894 |
| BcoDI | GTCTC | 2 cut(s) | 805, 1231 |
| BfaI | CTAG | 5 cut(s) | 266, 1029, 1134, 1200, 1308 |
| BfmI | CTRYAG | 1 cut(s) | 954 |
| BfuI | GTATCC | 1 cut(s) | 894 |
| BglII | AGATCT | 1 cut(s) | 472 |
| BisI | GCNGC | 1 cut(s) | 1144 |
| BlsI | GCNGC | 1 cut(s) | 1145 |
| Bme1390I | CCNGG | 3 cut(s) | 637, 945, 1266 |
| Bme18I | GGWCC | 2 cut(s) | 823, 947 |
| BmgT120I | GGNCC | 3 cut(s) | 257, 823, 947 |
| BmiI | GGNNCC | 4 cut(s) | 120, 658, 824, 898 |
| BmrFI | CCNGG | 3 cut(s) | 637, 945, 1266 |
| BmsI | GCATC | 3 cut(s) | 687, 1069, 1315 |
| BpiI | GAAGAC | 3 cut(s) | 134, 276, 314 |
| BpmI | CTGGAG | 3 cut(s) | 114, 883, 1042 |
| BpuEI | CTTGAG | 1 cut(s) | 1213 |
| BsaI | GGTCTC | 1 cut(s) | 1231 |
| BsaJI | CCNNGG | 4 cut(s) | 556, 635, 943, 1265 |
| BsaWI | WCCGGW | 1 cut(s) | 1122 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 87, 117 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 641, 893, 980, 1155, 1257 |
| Bse118I | RCCGGY | 2 cut(s) | 1209, 1218 |
| Bse1I | ACTGG | 2 cut(s) | 97, 900 |
| BseAI | TCCGGA | 1 cut(s) | 1122 |
| BseBI | CCWGG | 3 cut(s) | 637, 945, 1266 |
| BseDI | CCNNGG | 4 cut(s) | 556, 635, 943, 1265 |
| BseGI | GGATG | 1 cut(s) | 1088 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 641, 893, 980, 1155, 1257 |
| BseMII | CTCAG | 2 cut(s) | 611, 1020 |
| BseNI | ACTGG | 2 cut(s) | 97, 900 |
| BseRI | GAGGAG | 2 cut(s) | 113, 1177 |
| BseXI | GCAGC | 1 cut(s) | 1155 |
| BseYI | CCCAGC | 1 cut(s) | 31 |
| BsgI | GTGCAG | 1 cut(s) | 1032 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1318 |
| BshFI | GGCC | 5 cut(s) | 258, 453, 973, 1159, 1180 |
| BsiEI | CGRYCG | 1 cut(s) | 1318 |
| BsiSI | CCGG | 3 cut(s) | 1123, 1210, 1219 |
| BslFI | GGGAC | 2 cut(s) | 836, 996 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 641, 893, 980, 1155, 1257 |
| BsmAI | GTCTC | 2 cut(s) | 805, 1231 |
| BsmFI | GGGAC | 2 cut(s) | 836, 996 |
| BsnI | GGCC | 5 cut(s) | 258, 453, 973, 1159, 1180 |
| Bso31I | GGTCTC | 1 cut(s) | 1231 |
| Bsp13I | TCCGGA | 1 cut(s) | 1122 |
| Bsp1407I | TGTACA | 2 cut(s) | 85, 165 |
| Bsp143I | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| BspACI | CCGC | 1 cut(s) | 1314 |
| BspANI | GGCC | 5 cut(s) | 258, 453, 973, 1159, 1180 |
| BspCNI | CTCAG | 2 cut(s) | 612, 1019 |
| BspEI | TCCGGA | 1 cut(s) | 1122 |
| BspLI | GGNNCC | 4 cut(s) | 120, 658, 824, 898 |
| BspMAI | CTGCAG | 1 cut(s) | 958 |
| BspPI | GGATC | 1 cut(s) | 61 |
| BspTNI | GGTCTC | 1 cut(s) | 1231 |
| BsrFI | RCCGGY | 2 cut(s) | 1209, 1218 |
| BsrGI | TGTACA | 2 cut(s) | 85, 165 |
| BsrI | ACTGG | 2 cut(s) | 97, 900 |
| BssAI | RCCGGY | 2 cut(s) | 1209, 1218 |
| BssECI | CCNNGG | 4 cut(s) | 556, 635, 943, 1265 |
| BssMI | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| BssT1I | CCWWGG | 1 cut(s) | 556 |
| Bst2UI | CCWGG | 3 cut(s) | 637, 945, 1266 |
| Bst4CI | ACNGT | 3 cut(s) | 313, 380, 951 |
| Bst6I | CTCTTC | 2 cut(s) | 803, 1361 |
| BstAUI | TGTACA | 2 cut(s) | 85, 165 |
| BstC8I | GCNNGC | 3 cut(s) | 324, 532, 1220 |
| BstDEI | CTNAG | 3 cut(s) | 620, 1006, 1331 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1153 |
| BstF5I | GGATG | 1 cut(s) | 1088 |
| BstHHI | GCGC | 1 cut(s) | 1084 |
| BstKTI | GATC | 7 cut(s) | 45, 56, 331, 475, 586, 684, 1070 |
| BstMAI | GTCTC | 2 cut(s) | 805, 1231 |
| BstMBI | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| BstMCI | CGRYCG | 1 cut(s) | 1318 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 536, 774, 932, 1019, 1215, 1239 |
| BstNI | CCWGG | 3 cut(s) | 637, 945, 1266 |
| BstNSI | RCATGY | 3 cut(s) | 11, 172, 358 |
| BstSCI | CCNGG | 3 cut(s) | 635, 943, 1264 |
| BstSFI | CTRYAG | 1 cut(s) | 954 |
| BstV1I | GCAGC | 1 cut(s) | 1155 |
| BstV2I | GAAGAC | 3 cut(s) | 134, 276, 314 |
| BstX2I | RGATCY | 1 cut(s) | 472 |
| BstYI | RGATCY | 1 cut(s) | 472 |
| BsuI | GTATCC | 1 cut(s) | 894 |
| BsuRI | GGCC | 5 cut(s) | 258, 453, 973, 1159, 1180 |
| BtsCI | GGATG | 1 cut(s) | 1088 |
| BtsI | GCAGTG | 2 cut(s) | 55, 1240 |
| BtsIMutI | CAGTG | 4 cut(s) | 55, 691, 984, 1240 |
| Cac8I | GCNNGC | 3 cut(s) | 324, 532, 1220 |
| CfoI | GCGC | 1 cut(s) | 1084 |
| Cfr10I | RCCGGY | 2 cut(s) | 1209, 1218 |
| Cfr13I | GGNCC | 3 cut(s) | 257, 823, 947 |
| Csp6I | GTAC | 6 cut(s) | 86, 166, 194, 352, 1296, 1359 |
| CviAII | CATG | 5 cut(s) | 8, 169, 238, 355, 1046 |
| CviQI | GTAC | 6 cut(s) | 86, 166, 194, 352, 1296, 1359 |
| DdeI | CTNAG | 3 cut(s) | 620, 1006, 1331 |
| DpnI | GATC | 7 cut(s) | 44, 55, 330, 474, 585, 683, 1069 |
| DpnII | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| DraIII | CACNNNGTG | 1 cut(s) | 1049 |
| Eam1104I | CTCTTC | 2 cut(s) | 803, 1361 |
| EarI | CTCTTC | 2 cut(s) | 803, 1361 |
| Eco130I | CCWWGG | 1 cut(s) | 556 |
| Eco147I | AGGCCT | 2 cut(s) | 973, 1159 |
| Eco31I | GGTCTC | 1 cut(s) | 1231 |
| Eco47I | GGWCC | 2 cut(s) | 823, 947 |
| Eco57I | CTGAAG | 4 cut(s) | 827, 899, 960, 1284 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1153 |
| EcoO109I | RGGNCCY | 1 cut(s) | 823 |
| EcoRI | GAATTC | 1 cut(s) | 740 |
| EcoRII | CCWGG | 3 cut(s) | 635, 943, 1264 |
| EcoT14I | CCWWGG | 1 cut(s) | 556 |
| ErhI | CCWWGG | 1 cut(s) | 556 |
| FaeI | CATG | 5 cut(s) | 11, 172, 241, 358, 1049 |
| FaqI | GGGAC | 2 cut(s) | 836, 996 |
| FatI | CATG | 5 cut(s) | 7, 168, 237, 354, 1045 |
| Fnu4HI | GCNGC | 1 cut(s) | 1144 |
| FokI | GGATG | 1 cut(s) | 1075 |
| Fsp4HI | GCNGC | 1 cut(s) | 1144 |
| FspBI | CTAG | 5 cut(s) | 266, 1029, 1134, 1200, 1308 |
| GlaI | GCGC | 1 cut(s) | 1083 |
| GluI | GCNGC | 1 cut(s) | 1144 |
| GsaI | CCCAGC | 1 cut(s) | 35 |
| GsuI | CTGGAG | 3 cut(s) | 114, 883, 1042 |
| HaeIII | GGCC | 5 cut(s) | 258, 453, 973, 1159, 1180 |
| HapII | CCGG | 3 cut(s) | 1123, 1210, 1219 |
| HhaI | GCGC | 1 cut(s) | 1084 |
| Hin1II | CATG | 5 cut(s) | 11, 172, 241, 358, 1049 |
| Hin6I | GCGC | 1 cut(s) | 1082 |
| HinP1I | GCGC | 1 cut(s) | 1082 |
| HincII | GTYRAC | 2 cut(s) | 547, 1248 |
| HindII | GTYRAC | 2 cut(s) | 547, 1248 |
| HinfI | GANTC | 7 cut(s) | 155, 514, 590, 1002, 1109, 1119, 1274 |
| HpaI | GTTAAC | 1 cut(s) | 547 |
| HpaII | CCGG | 3 cut(s) | 1123, 1210, 1219 |
| HphI | GGTGA | 5 cut(s) | 236, 368, 545, 1061, 1205 |
| Hpy166II | GTNNAC | 5 cut(s) | 194, 300, 547, 1043, 1248 |
| Hpy188I | TCNGA | 6 cut(s) | 215, 294, 519, 621, 739, 1114 |
| Hpy8I | GTNNAC | 5 cut(s) | 194, 300, 547, 1043, 1248 |
| HpyAV | CCTTC | 5 cut(s) | 260, 641, 833, 984, 1026 |
| HpyCH4III | ACNGT | 3 cut(s) | 313, 380, 951 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 536, 774, 932, 1019, 1215, 1239 |
| HpyF3I | CTNAG | 3 cut(s) | 620, 1006, 1331 |
| Hsp92II | CATG | 5 cut(s) | 11, 172, 241, 358, 1049 |
| HspAI | GCGC | 1 cut(s) | 1082 |
| Kpn2I | TCCGGA | 1 cut(s) | 1122 |
| KroI | GCCGGC | 1 cut(s) | 1218 |
| KroNI | GCCGGC | 1 cut(s) | 1220 |
| KspAI | GTTAAC | 1 cut(s) | 547 |
| Kzo9I | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| LmnI | GCTCC | 5 cut(s) | 4, 64, 902, 1190, 1335 |
| Lsp1109I | GCAGC | 1 cut(s) | 1155 |
| LweI | GCATC | 3 cut(s) | 687, 1069, 1315 |
| MaeI | CTAG | 5 cut(s) | 266, 1029, 1134, 1200, 1308 |
| MaeIII | GTNAC | 1 cut(s) | 380 |
| MalI | GATC | 7 cut(s) | 44, 55, 330, 474, 585, 683, 1069 |
| MboI | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| MboII | GAAGA | 9 cut(s) | 139, 167, 281, 319, 365, 467, 706, 794, 820 |
| MfeI | CAATTG | 1 cut(s) | 708 |
| MflI | RGATCY | 1 cut(s) | 472 |
| MluCI | AATT | 5 cut(s) | 332, 708, 740, 848, 891 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MroI | TCCGGA | 1 cut(s) | 1122 |
| MroNI | GCCGGC | 1 cut(s) | 1218 |
| MseI | TTAA | 7 cut(s) | 27, 81, 204, 317, 335, 404, 546 |
| MslI | CAYNNNNRTG | 1 cut(s) | 558 |
| MspI | CCGG | 3 cut(s) | 1123, 1210, 1219 |
| MspR9I | CCNGG | 3 cut(s) | 637, 945, 1266 |
| MunI | CAATTG | 1 cut(s) | 708 |
| MvaI | CCWGG | 3 cut(s) | 637, 945, 1266 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 536, 774, 932, 1019, 1215, 1239 |
| NaeI | GCCGGC | 1 cut(s) | 1220 |
| NdeII | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| NgoMIV | GCCGGC | 1 cut(s) | 1218 |
| NlaIII | CATG | 5 cut(s) | 11, 172, 241, 358, 1049 |
| NlaIV | GGNNCC | 4 cut(s) | 120, 658, 824, 898 |
| NmuCI | GTSAC | 1 cut(s) | 380 |
| NspI | RCATGY | 3 cut(s) | 11, 172, 358 |
| OliI | CACNNNNGTG | 1 cut(s) | 558 |
| PceI | AGGCCT | 2 cut(s) | 973, 1159 |
| PdiI | GCCGGC | 1 cut(s) | 1220 |
| PfeI | GAWTC | 6 cut(s) | 514, 590, 1002, 1109, 1119, 1274 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 893 |
| PkrI | GCNGC | 1 cut(s) | 1145 |
| PleI | GAGTC | 1 cut(s) | 163 |
| PpsI | GAGTC | 1 cut(s) | 163 |
| PpuMI | RGGWCCY | 1 cut(s) | 823 |
| PshBI | ATTAAT | 2 cut(s) | 81, 335 |
| Psp5II | RGGWCCY | 1 cut(s) | 823 |
| Psp6I | CCWGG | 3 cut(s) | 635, 943, 1264 |
| PspFI | CCCAGC | 1 cut(s) | 31 |
| PspGI | CCWGG | 3 cut(s) | 635, 943, 1264 |
| PspN4I | GGNNCC | 4 cut(s) | 120, 658, 824, 898 |
| PspPI | GGNCC | 3 cut(s) | 257, 823, 947 |
| PspPPI | RGGWCCY | 1 cut(s) | 823 |
| PstI | CTGCAG | 1 cut(s) | 958 |
| PsuI | RGATCY | 1 cut(s) | 472 |
| RsaI | GTAC | 6 cut(s) | 87, 167, 195, 353, 1297, 1360 |
| RsaNI | GTAC | 6 cut(s) | 86, 166, 194, 352, 1296, 1359 |
| RseI | CAYNNNNRTG | 1 cut(s) | 558 |
| SaqAI | TTAA | 7 cut(s) | 27, 81, 204, 317, 335, 404, 546 |
| SatI | GCNGC | 1 cut(s) | 1144 |
| Sau3AI | GATC | 7 cut(s) | 42, 53, 328, 472, 583, 681, 1067 |
| Sau96I | GGNCC | 3 cut(s) | 257, 823, 947 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 3 cut(s) | 637, 945, 1266 |
| SfaNI | GCATC | 3 cut(s) | 687, 1069, 1315 |
| SfcI | CTRYAG | 1 cut(s) | 954 |
| SinI | GGWCC | 2 cut(s) | 823, 947 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 558 |
| SmlI | CTYRAG | 1 cut(s) | 1228 |
| SmoI | CTYRAG | 1 cut(s) | 1228 |
| Sse9I | AATT | 5 cut(s) | 332, 708, 740, 848, 891 |
| SseBI | AGGCCT | 2 cut(s) | 973, 1159 |
| SsiI | CCGC | 1 cut(s) | 1314 |
| SspMI | CTAG | 5 cut(s) | 266, 1029, 1134, 1200, 1308 |
| StuI | AGGCCT | 2 cut(s) | 973, 1159 |
| StyD4I | CCNGG | 3 cut(s) | 635, 943, 1264 |
| StyI | CCWWGG | 1 cut(s) | 556 |
| TaaI | ACNGT | 3 cut(s) | 313, 380, 951 |
| TaqI | TCGA | 1 cut(s) | 994 |
| TasI | AATT | 5 cut(s) | 332, 708, 740, 848, 891 |
| TatI | WGTACW | 3 cut(s) | 85, 165, 351 |
| TfiI | GAWTC | 6 cut(s) | 514, 590, 1002, 1109, 1119, 1274 |
| Tru1I | TTAA | 7 cut(s) | 27, 81, 204, 317, 335, 404, 546 |
| Tru9I | TTAA | 7 cut(s) | 27, 81, 204, 317, 335, 404, 546 |
| TscAI | CASTG | 4 cut(s) | 55, 691, 984, 1240 |
| TseFI | GTSAC | 1 cut(s) | 380 |
| TseI | GCWGC | 1 cut(s) | 1143 |
| Tsp45I | GTSAC | 1 cut(s) | 380 |
| TspDTI | ATGAA | 4 cut(s) | 789, 820, 1119, 1287 |
| TspRI | CASTG | 4 cut(s) | 55, 691, 984, 1240 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 893 |
| VpaK11BI | GGWCC | 2 cut(s) | 823, 947 |
| VspI | ATTAAT | 2 cut(s) | 81, 335 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1153 |
| XapI | RAATTY | 1 cut(s) | 740 |
| XbaI | TCTAGA | 3 cut(s) | 1028, 1133, 1307 |
| XceI | RCATGY | 3 cut(s) | 11, 172, 358 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1262 |
| XspI | CTAG | 5 cut(s) | 266, 1029, 1134, 1200, 1308 |