FvH4_2g21773

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000429
Physical Location & Seq
Forward (+)
18021938 .. 18024595
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21773.t1

Sequence Viewer

Length: 1014 bp
ATGGAGAAGCTCATCTCACATAGAAGTAACTTGCAATCTGTACCTCAACTTTACATATTACCCCCGGAAACTAGACCCGGCAATACACAAGTTTTGATATTTGAAACCATTCCTGTTATTGATTTTCTGCAACTTGGTACTGATAGAGCAGAACTGATTAAGCAAGTTACAAGGGCCGCCCAAGAATTTGGATTCTTCCAGTTGATCAATCATGGTGTTCCAGATACCTTGTTGGATAAGGTATTGAATGTAGCCAATGAGTTCTTCGAGCTGCCTGGTGAAGACAAAGAAAGTGTATATTCCGAAAATCCTATGCAAAGCTGTAGATTATACACCAGCATCGATTATATGAGAGAACGGGTACATTACTGGAGAGACGTTTTGCGACACCCTTGTCATCCTCTGGAGGAGCACATCCAATTCTGGCCTCAGAAACCACCTCAATATCGAGATGTGGTTGGGAGTTACTCAGTGGAAGTGAGGAAGCTGGGTTTGTGTCTTTTGGATCTCATTTCTGAAGGATTAGGACTTGAATCTGGCTTTTTTGTGGGTGAACTTAGCCAAGTGCAAATAATGGCTACAAATCATTACCCACTATGCCCAGATCCAAGCTTAACCTTGGGATTACCTAAACATAGTGATGTCAACCTCATCACTCTTCTTATACAAGGAGAAGTTTCTGGACTTCAGGTTTTGAAAGATGAGCAGTGGTTAACTGTTCAGCCTGTCCCTAATGCATTTGTAGTTAATATTGGCCACATCTTACAGATTATCAGCAATGGGAAGCTATGCAGTGCCGAGCACAGGGTTGTGACAAACGAAAAGTCTTCAAGGACAACAGTTGCAAGCTACATCAATCCTTCCTTCAACTGCCAAATTGAACCTGCAAAAGCACTTTTGGCTGAGGACTCCAGTGCTCCACTCTACAGAGCTTTTGCTTACAAGGATTTTCTTCGCACCTATGTAACAGATACTCATCAAGGAGTACCACCACTTGAGCGTTACGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

338

Amino Acids

38.2

Weight (kDa)

5.47

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 37 - 144 2.6e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 190 - 287 1.2e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 892
AciI CCGC 1 cut(s) 177
AclWI GGATC 2 cut(s) 513, 599
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 537, 671
AfaI GTAC 4 cut(s) 42, 139, 363, 987
AfiI CCNNNNNNNGG 1 cut(s) 804
AgsI TTSAA 7 cut(s) 104, 247, 533, 697, 831, 868, 881
AjnI CCWGG 1 cut(s) 274
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 8 cut(s) 10, 271, 321, 487, 612, 787, 849, 932
AluI AGCT 8 cut(s) 10, 271, 321, 487, 612, 787, 849, 932
Alw21I GWGCWC 3 cut(s) 414, 804, 919
Alw26I GTCTC 1 cut(s) 369
AlwI GGATC 2 cut(s) 513, 599
AoxI GGCC 3 cut(s) 174, 425, 754
ApeKI GCWGC 1 cut(s) 271
ApoI RAATTY 1 cut(s) 185
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 2 cut(s) 65, 78
AsuHPI GGTGA 2 cut(s) 290, 563
BalI TGGCCA 1 cut(s) 756
BbsI GAAGAC 2 cut(s) 288, 819
Bbv12I GWGCWC 3 cut(s) 414, 804, 919
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 1 cut(s) 258
BciT130I CCWGG 1 cut(s) 276
BclI TGATCA 1 cut(s) 204
BcnI CCSGG 2 cut(s) 65, 78
BcoDI GTCTC 1 cut(s) 369
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 2 cut(s) 322, 925
BfuAI ACCTGC 1 cut(s) 892
BisI GCNGC 2 cut(s) 177, 272
BlsI GCNGC 2 cut(s) 178, 273
Bme1390I CCNGG 3 cut(s) 65, 78, 276
BmgT120I GGNCC 1 cut(s) 174
BmrFI CCNGG 3 cut(s) 65, 78, 276
BmsI GCATC 1 cut(s) 348
BpiI GAAGAC 2 cut(s) 288, 819
BpmI CTGGAG 3 cut(s) 391, 425, 895
Bpu10I CCTNAGC 1 cut(s) 903
BpuMI CCSGG 2 cut(s) 65, 78
Bsa29I ATCGAT 1 cut(s) 342
BsaBI GATNNNNATC 1 cut(s) 975
BsaJI CCNNGG 2 cut(s) 63, 618
Bsc4I CCNNNNNNNGG 1 cut(s) 804
Bse1I ACTGG 3 cut(s) 199, 374, 912
Bse3DI GCAATG 1 cut(s) 784
Bse8I GATNNNNATC 1 cut(s) 975
BseBI CCWGG 1 cut(s) 276
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 63, 618
BseGI GGATG 2 cut(s) 397, 414
BseJI GATNNNNATC 1 cut(s) 975
BseLI CCNNNNNNNGG 1 cut(s) 804
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 3 cut(s) 443, 483, 894
BseNI ACTGG 3 cut(s) 199, 374, 912
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 258
BseYI CCCAGC 1 cut(s) 487
BshFI GGCC 3 cut(s) 176, 427, 756
BshVI ATCGAT 1 cut(s) 342
BsiHKAI GWGCWC 3 cut(s) 414, 804, 919
BsiSI CCGG 2 cut(s) 65, 78
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 1 cut(s) 804
BsmAI GTCTC 1 cut(s) 369
BsmBI CGTCTC 1 cut(s) 369
BsmFI GGGAC 1 cut(s) 713
BsnI GGCC 3 cut(s) 176, 427, 756
Bsp1286I GDGCHC 3 cut(s) 414, 804, 919
Bsp143I GATC 3 cut(s) 204, 505, 604
BspACI CCGC 1 cut(s) 177
BspANI GGCC 3 cut(s) 176, 427, 756
BspCNI CTCAG 3 cut(s) 442, 482, 895
BspDI ATCGAT 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 892
BspPI GGATC 2 cut(s) 513, 599
BsrDI GCAATG 1 cut(s) 784
BsrI ACTGG 3 cut(s) 199, 374, 912
BssECI CCNNGG 2 cut(s) 63, 618
BssMI GATC 3 cut(s) 204, 505, 604
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 718, 841
Bst6I CTCTTC 1 cut(s) 663
BstC8I GCNNGC 1 cut(s) 847
BstDEI CTNAG 4 cut(s) 429, 469, 557, 903
BstF5I GGATG 2 cut(s) 397, 414
BstKTI GATC 3 cut(s) 207, 508, 607
BstMAI GTCTC 1 cut(s) 369
BstMBI GATC 3 cut(s) 204, 505, 604
BstMWI GCNNNNNNNGC 1 cut(s) 899
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 3 cut(s) 63, 76, 274
BstSFI CTRYAG 2 cut(s) 322, 925
BstV1I GCAGC 1 cut(s) 258
BstV2I GAAGAC 2 cut(s) 288, 819
BstX2I RGATCY 2 cut(s) 505, 604
BstXI CCANNNNNNTGG 1 cut(s) 188
BstYI RGATCY 2 cut(s) 505, 604
Bsu15I ATCGAT 1 cut(s) 342
BsuRI GGCC 3 cut(s) 176, 427, 756
BsuTUI ATCGAT 1 cut(s) 342
BtsCI GGATG 2 cut(s) 397, 414
BtsI GCAGTG 2 cut(s) 713, 799
BtsIMutI CAGTG 4 cut(s) 477, 713, 799, 919
BveI ACCTGC 1 cut(s) 892
Cac8I GCNNGC 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 342
Csp6I GTAC 4 cut(s) 41, 138, 362, 986
CviAII CATG 1 cut(s) 212
CviQI GTAC 4 cut(s) 41, 138, 362, 986
DdeI CTNAG 4 cut(s) 429, 469, 557, 903
DpnI GATC 3 cut(s) 206, 507, 606
DpnII GATC 3 cut(s) 204, 505, 604
DrdI GACNNNNNNGTC 1 cut(s) 393
DseDI GACNNNNNNGTC 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco130I CCWWGG 1 cut(s) 618
Eco57I CTGAAG 2 cut(s) 537, 671
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 739
ErhI CCWWGG 1 cut(s) 618
Esp3I CGTCTC 1 cut(s) 369
FaeI CATG 1 cut(s) 215
FaqI GGGAC 1 cut(s) 713
FatI CATG 1 cut(s) 211
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 177, 272
FokI GGATG 2 cut(s) 384, 401
Fsp4HI GCNGC 2 cut(s) 177, 272
FspBI CTAG 1 cut(s) 72
GluI GCNGC 2 cut(s) 177, 272
GsaI CCCAGC 1 cut(s) 491
GsuI CTGGAG 3 cut(s) 391, 425, 895
HaeIII GGCC 3 cut(s) 176, 427, 756
HapII CCGG 2 cut(s) 65, 78
Hin1II CATG 1 cut(s) 215
HincII GTYRAC 2 cut(s) 646, 714
HindII GTYRAC 2 cut(s) 646, 714
HindIII AAGCTT 1 cut(s) 610
HinfI GANTC 3 cut(s) 192, 533, 908
HpaI GTTAAC 1 cut(s) 714
HpaII CCGG 2 cut(s) 65, 78
HphI GGTGA 2 cut(s) 290, 563
Hpy166II GTNNAC 3 cut(s) 554, 646, 714
Hpy188I TCNGA 3 cut(s) 304, 432, 517
Hpy188III TCNNGA 4 cut(s) 221, 404, 449, 681
Hpy8I GTNNAC 3 cut(s) 554, 646, 714
HpyAV CCTTC 3 cut(s) 512, 870, 874
HpyCH4III ACNGT 2 cut(s) 718, 841
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 8 cut(s) 34, 130, 316, 568, 737, 792, 845, 887
HpyF10VI GCNNNNNNNGC 1 cut(s) 899
HpyF3I CTNAG 4 cut(s) 429, 469, 557, 903
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 204
KspAI GTTAAC 1 cut(s) 714
Kzo9I GATC 3 cut(s) 204, 505, 604
LmnI GCTCC 2 cut(s) 409, 922
Lsp1109I GCAGC 1 cut(s) 258
LweI GCATC 1 cut(s) 348
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 6 cut(s) 26, 166, 464, 811, 964, 1001
MalI GATC 3 cut(s) 206, 507, 606
MboI GATC 3 cut(s) 204, 505, 604
MboII GAAGA 6 cut(s) 187, 256, 293, 650, 819, 944
MflI RGATCY 2 cut(s) 505, 604
MhlI GDGCHC 3 cut(s) 414, 804, 919
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 3 cut(s) 185, 419, 876
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 1 cut(s) 902
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 8 cut(s) 54, 400, 411, 438, 450, 474, 659, 898
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 756
MseI TTAA 4 cut(s) 159, 614, 713, 747
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 1 cut(s) 756
MspI CCGG 2 cut(s) 65, 78
MspR9I CCNGG 3 cut(s) 65, 78, 276
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 899
NciI CCSGG 2 cut(s) 65, 78
NdeII GATC 3 cut(s) 204, 505, 604
NlaIII CATG 1 cut(s) 215
NmeAIII GCCGAG 1 cut(s) 823
NmuCI GTSAC 1 cut(s) 811
NsiI ATGCAT 1 cut(s) 739
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 192, 533
PkrI GCNGC 2 cut(s) 178, 273
PleI GAGTC 1 cut(s) 902
PpsI GAGTC 1 cut(s) 902
Psp6I CCWGG 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 487
PspGI CCWGG 1 cut(s) 274
PspPI GGNCC 1 cut(s) 174
PsuI RGATCY 2 cut(s) 505, 604
RsaI GTAC 4 cut(s) 42, 139, 363, 987
RsaNI GTAC 4 cut(s) 41, 138, 362, 986
RseI CAYNNNNRTG 1 cut(s) 639
SaqAI TTAA 4 cut(s) 159, 614, 713, 747
SatI GCNGC 2 cut(s) 177, 272
Sau3AI GATC 3 cut(s) 204, 505, 604
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 902
ScrFI CCNGG 3 cut(s) 65, 78, 276
SduI GDGCHC 3 cut(s) 414, 804, 919
SfaNI GCATC 1 cut(s) 348
SfcI CTRYAG 2 cut(s) 322, 925
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
Sse9I AATT 3 cut(s) 185, 419, 876
SsiI CCGC 1 cut(s) 177
SspI AATATT 1 cut(s) 751
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 63, 76, 274
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 2 cut(s) 718, 841
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 267, 342, 448
TasI AATT 3 cut(s) 185, 419, 876
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 192, 533
Tru1I TTAA 4 cut(s) 159, 614, 713, 747
Tru9I TTAA 4 cut(s) 159, 614, 713, 747
TscAI CASTG 4 cut(s) 477, 713, 799, 919
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 811
TspRI CASTG 4 cut(s) 477, 713, 799, 919
XapI RAATTY 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.