MD05G1199100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000429
Physical Location & Seq
Reverse (-)
32695528 .. 32698795
3268 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1199100.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGAGAAGCTTGTTTCAAGCTGGTACAAAGATGGAACCTTGCCGGAGAGTTTCATATGGCCACCGGATTCAAGACCCGGGAAGCTTGCAGCTGCTCCTTCATGCAAGAACATTCCGGTTATTGATCTTGGTGGAGCTGAAGGGGGTGATCATACCTACATCATTCACCAAATACTCGAGGCCAGTCAAGAATTCGGATTTTTTCAGGTAATCAACCATGGCATCTCAGATACTCTGTTAAGTGACACAATGAGTGTGTTGAAGGAGTTCTTTGAGTTGCCGCTAGAGGACAAGGCAAGCGTTTACTCTGAGGACCCCCAAAAAAGTTGCAAGCTCTTCAGCAGCAGCGTAAATTATGATGCGGAAGATGTTCATCTTTGGCGCGATGTCCTGCGACACCCTTGTCATCCATTGGTGGAATGCTTGCAAGATTGGCCTCAACAGCCAAGTAGATATCGAGAGCTTGTTGGGACATGTTCTACACAAGTGAAGAAACTGGCTCTGAACGTTTTGGAGCTAATCAGTGAAGGACTTGGGCTCGGAACTGGGTATTTCCGAAACGAACTTAGCCAAAACTTGATACTCTCAGTTAATCATTACCCGCCTTGCCCCGACCCAAGTTTGACATTAGGATTAACTAAACATTGTGACCCAAACCTCATAACCATCTTAATGCAAGAGGATCATGTAAATGGACTTCAAGTTTTCAAAGACGGGGAATGGATGGGCGTGGAGCCTATTTCACATGCACTTGTGGTTAACATAGGCTATCAGTTACACATCATCAGTAATGGGAAGCTGAAAAGTTCTGAACATCGGGCTGTGACGAATCCATGTAATGCTAGGACATCTGCAGGATTTTTCGTTGCGCCTTCTTATGTTTGCGTTGTAGAACCTGCAAGAGCTCTTGTTAGTGCAAGCAATCCCCAACTATATAAAAGTTTCCAGTACGAAGAGTTTCTTCGCAACTTTAATACGATGGTATTCGAAGGTAAAAGCGAATTTGCAATGGAAAGCTTTAAGCTCCAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

38.5

Weight (kDa)

5.29

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 147 5.1e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 193 - 291 2.1e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 402
Acc36I ACCTGC 1 cut(s) 904
AccII CGCG 1 cut(s) 384
AciI CCGC 3 cut(s) 281, 362, 602
AclI AACGTT 1 cut(s) 507
AclWI GGATC 1 cut(s) 690
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 191, 1001
AcuI CTGAAG 2 cut(s) 159, 322
AfaI GTAC 2 cut(s) 26, 950
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 5 cut(s) 18, 72, 262, 701, 709
Alw21I GWGCWC 1 cut(s) 907
AlwI GGATC 1 cut(s) 690
Ama87I CYCGRG 2 cut(s) 77, 176
AoxI GGCC 3 cut(s) 59, 180, 434
ApeKI GCWGC 4 cut(s) 89, 92, 342, 345
ApoI RAATTY 2 cut(s) 191, 1001
Asp700I GAANNNNTTC 3 cut(s) 266, 369, 957
AspLEI GCGC 2 cut(s) 384, 871
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 78, 79
AsuHPI GGTGA 2 cut(s) 158, 158
AsuII TTCGAA 1 cut(s) 987
AvaI CYCGRG 2 cut(s) 77, 176
AvaII GGWCC 1 cut(s) 313
BalI TGGCCA 1 cut(s) 61
BanII GRGCYC 2 cut(s) 540, 907
BarI GAAGNNNNNNTAC 2 cut(s) 681, 713
Bbv12I GWGCWC 1 cut(s) 907
BbvI GCAGC 4 cut(s) 79, 101, 354, 357
BccI CCATC 4 cut(s) 26, 674, 718, 973
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 2 cut(s) 78, 79
BfaI CTAG 2 cut(s) 284, 843
BfmI CTRYAG 1 cut(s) 852
BfuAI ACCTGC 1 cut(s) 904
BisI GCNGC 5 cut(s) 90, 93, 281, 343, 346
BlsI GCNGC 5 cut(s) 91, 94, 282, 344, 347
Bme1390I CCNGG 2 cut(s) 78, 79
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 2 cut(s) 77, 176
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 3 cut(s) 37, 315, 735
BmrFI CCNGG 2 cut(s) 78, 79
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 2 cut(s) 231, 349
BmuI ACTGGG 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 987
BpuMI CCSGG 2 cut(s) 78, 79
BsaBI GATNNNNATC 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 77, 217
BsaWI WCCGGW 2 cut(s) 64, 115
Bse1I ACTGG 4 cut(s) 183, 501, 550, 946
Bse3DI GCAATG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 77, 217
BseGI GGATG 2 cut(s) 406, 729
BseJI GATNNNNATC 1 cut(s) 372
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 3 cut(s) 240, 300, 600
BseNI ACTGG 4 cut(s) 183, 501, 550, 946
BseXI GCAGC 4 cut(s) 79, 101, 354, 357
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 3 cut(s) 61, 182, 436
BsiHKAI GWGCWC 1 cut(s) 907
BsiHKCI CYCGRG 2 cut(s) 77, 176
BsiSI CCGG 4 cut(s) 44, 65, 78, 116
BslFI GGGAC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 3 cut(s) 61, 182, 436
BsoBI CYCGRG 2 cut(s) 77, 176
Bsp119I TTCGAA 1 cut(s) 987
Bsp1286I GDGCHC 2 cut(s) 540, 907
Bsp143I GATC 3 cut(s) 124, 148, 682
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 3 cut(s) 281, 362, 602
BspANI GGCC 3 cut(s) 61, 182, 436
BspCNI CTCAG 3 cut(s) 239, 301, 599
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 3 cut(s) 37, 315, 735
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 1 cut(s) 690
BspQI GCTCTTC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 987
BsrDI GCAATG 1 cut(s) 1014
BsrI ACTGG 4 cut(s) 183, 501, 550, 946
BssECI CCNNGG 2 cut(s) 77, 217
BssMI GATC 3 cut(s) 124, 148, 682
BssT1I CCWWGG 1 cut(s) 217
Bst6I CTCTTC 2 cut(s) 341, 948
BstBI TTCGAA 1 cut(s) 987
BstC8I GCNNGC 5 cut(s) 87, 298, 332, 425, 919
BstDEI CTNAG 5 cut(s) 226, 309, 566, 586, 1032
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 2 cut(s) 406, 729
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 2 cut(s) 384, 871
BstKTI GATC 3 cut(s) 127, 151, 685
BstMBI GATC 3 cut(s) 124, 148, 682
BstMWI GCNNNNNNNGC 2 cut(s) 433, 442
BstNSI RCATGY 2 cut(s) 477, 749
BstSCI CCNGG 2 cut(s) 76, 77
BstSFI CTRYAG 1 cut(s) 852
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 4 cut(s) 79, 101, 354, 357
BsuRI GGCC 3 cut(s) 61, 182, 436
BtgI CCRYGG 1 cut(s) 217
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 2 cut(s) 406, 729
BtsIMutI CAGTG 1 cut(s) 529
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 5 cut(s) 87, 298, 332, 425, 919
CfoI GCGC 2 cut(s) 384, 871
Cfr13I GGNCC 1 cut(s) 313
Cfr9I CCCGGG 1 cut(s) 77
Csp6I GTAC 2 cut(s) 25, 949
CviAII CATG 6 cut(s) 102, 218, 474, 686, 746, 834
CviQI GTAC 2 cut(s) 25, 949
DdeI CTNAG 5 cut(s) 226, 309, 566, 586, 1032
DpnI GATC 3 cut(s) 126, 150, 684
DpnII GATC 3 cut(s) 124, 148, 682
DrdI GACNNNNNNGTC 1 cut(s) 402
DseDI GACNNNNNNGTC 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 59
Eam1104I CTCTTC 2 cut(s) 341, 948
EarI CTCTTC 2 cut(s) 341, 948
Ecl136II GAGCTC 1 cut(s) 905
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 2 cut(s) 540, 907
Eco32I GATATC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 905
Eco57I CTGAAG 2 cut(s) 159, 322
Eco88I CYCGRG 2 cut(s) 77, 176
EcoICRI GAGCTC 1 cut(s) 905
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 191
EcoRV GATATC 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 2 cut(s) 540, 907
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 6 cut(s) 105, 221, 477, 689, 749, 837
FalI AAGNNNNNCTT 4 cut(s) 254, 286, 945, 977
FaqI GGGAC 1 cut(s) 484
FatI CATG 6 cut(s) 101, 217, 473, 685, 745, 833
FauI CCCGC 1 cut(s) 609
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 5 cut(s) 90, 93, 281, 343, 346
FokI GGATG 2 cut(s) 393, 736
FriOI GRGCYC 2 cut(s) 540, 907
Fsp4HI GCNGC 5 cut(s) 90, 93, 281, 343, 346
FspBI CTAG 2 cut(s) 284, 843
GlaI GCGC 2 cut(s) 383, 870
GluI GCNGC 5 cut(s) 90, 93, 281, 343, 346
HaeIII GGCC 3 cut(s) 61, 182, 436
HapII CCGG 4 cut(s) 44, 65, 78, 116
HhaI GCGC 2 cut(s) 384, 871
Hin1II CATG 6 cut(s) 105, 221, 477, 689, 749, 837
Hin6I GCGC 2 cut(s) 382, 869
HinP1I GCGC 2 cut(s) 382, 869
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 3 cut(s) 8, 83, 1015
HinfI GANTC 2 cut(s) 68, 829
HpaI GTTAAC 1 cut(s) 760
HpaII CCGG 4 cut(s) 44, 65, 78, 116
HphI GGTGA 2 cut(s) 158, 158
Hpy166II GTNNAC 2 cut(s) 304, 760
Hpy188I TCNGA 7 cut(s) 197, 229, 310, 504, 542, 557, 811
Hpy188III TCNNGA 3 cut(s) 72, 188, 458
Hpy8I GTNNAC 2 cut(s) 304, 760
HpyAV CCTTC 6 cut(s) 108, 134, 256, 521, 882, 983
HpyCH4IV ACGT 1 cut(s) 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 433, 442
HpyF3I CTNAG 5 cut(s) 226, 309, 566, 586, 1032
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 6 cut(s) 105, 221, 477, 689, 749, 837
HspAI GCGC 2 cut(s) 382, 869
Ksp22I TGATCA 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 760
Kzo9I GATC 3 cut(s) 124, 148, 682
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 5 cut(s) 100, 134, 514, 733, 1029
Lsp1109I GCAGC 4 cut(s) 79, 101, 354, 357
LweI GCATC 2 cut(s) 231, 349
MaeI CTAG 2 cut(s) 284, 843
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 4 cut(s) 242, 647, 774, 823
MalI GATC 3 cut(s) 126, 150, 684
MboI GATC 3 cut(s) 124, 148, 682
MboII GAAGA 5 cut(s) 328, 377, 502, 953, 965
MhlI GDGCHC 2 cut(s) 540, 907
MlsI TGGCCA 1 cut(s) 61
MluCI AATT 3 cut(s) 191, 352, 1001
MluNI TGGCCA 1 cut(s) 61
MnlI CCTC 6 cut(s) 172, 280, 304, 447, 668, 673
Mox20I TGGCCA 1 cut(s) 61
MroXI GAANNNNTTC 3 cut(s) 266, 369, 957
MscI TGGCCA 1 cut(s) 61
MseI TTAA 7 cut(s) 239, 591, 635, 671, 759, 972, 1020
MslI CAYNNNNRTG 2 cut(s) 671, 690
Msp20I TGGCCA 1 cut(s) 61
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 4 cut(s) 44, 65, 78, 116
MspR9I CCNGG 2 cut(s) 78, 79
Mva1269I GAATGC 1 cut(s) 425
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 433, 442
NciI CCSGG 2 cut(s) 78, 79
NcoI CCATGG 1 cut(s) 217
NdeI CATATG 1 cut(s) 56
NdeII GATC 3 cut(s) 124, 148, 682
NlaIII CATG 6 cut(s) 105, 221, 477, 689, 749, 837
NlaIV GGNNCC 3 cut(s) 37, 315, 735
NmuCI GTSAC 3 cut(s) 242, 647, 823
NspI RCATGY 2 cut(s) 477, 749
NspV TTCGAA 1 cut(s) 987
PaeR7I CTCGAG 1 cut(s) 176
PciI ACATGT 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 341
PctI GAATGC 1 cut(s) 425
PdmI GAANNNNTTC 3 cut(s) 266, 369, 957
PfeI GAWTC 2 cut(s) 68, 829
PkrI GCNGC 5 cut(s) 91, 94, 282, 344, 347
PpuMI RGGWCCY 1 cut(s) 313
PscI ACATGT 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 907
Psp1406I AACGTT 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 37, 315, 735
PspPI GGNCC 1 cut(s) 313
PspPPI RGGWCCY 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 1 cut(s) 856
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 2 cut(s) 26, 950
RsaNI GTAC 2 cut(s) 25, 949
RseI CAYNNNNRTG 2 cut(s) 671, 690
SacI GAGCTC 1 cut(s) 907
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 7 cut(s) 239, 591, 635, 671, 759, 972, 1020
SatI GCNGC 5 cut(s) 90, 93, 281, 343, 346
Sau3AI GATC 3 cut(s) 124, 148, 682
Sau96I GGNCC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 78, 79
SduI GDGCHC 2 cut(s) 540, 907
SfaNI GCATC 2 cut(s) 231, 349
SfcI CTRYAG 1 cut(s) 852
Sfr274I CTCGAG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 987
SinI GGWCC 1 cut(s) 313
SlaI CTCGAG 1 cut(s) 176
SmaI CCCGGG 1 cut(s) 79
SmiMI CAYNNNNRTG 2 cut(s) 671, 690
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 3 cut(s) 191, 352, 1001
SsiI CCGC 3 cut(s) 281, 362, 602
SspMI CTAG 2 cut(s) 284, 843
SstI GAGCTC 1 cut(s) 907
StyD4I CCNGG 2 cut(s) 76, 77
StyI CCWWGG 1 cut(s) 217
TaiI ACGT 1 cut(s) 510
TaqI TCGA 3 cut(s) 177, 457, 987
TasI AATT 3 cut(s) 191, 352, 1001
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 2 cut(s) 68, 829
Tru1I TTAA 7 cut(s) 239, 591, 635, 671, 759, 972, 1020
Tru9I TTAA 7 cut(s) 239, 591, 635, 671, 759, 972, 1020
TscAI CASTG 1 cut(s) 529
TseFI GTSAC 3 cut(s) 242, 647, 823
TseI GCWGC 4 cut(s) 89, 92, 342, 345
Tsp45I GTSAC 3 cut(s) 242, 647, 823
TspDTI ATGAA 3 cut(s) 43, 90, 362
TspMI CCCGGG 1 cut(s) 77
TspRI CASTG 1 cut(s) 529
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 2 cut(s) 191, 1001
XceI RCATGY 2 cut(s) 477, 749
XhoI CTCGAG 1 cut(s) 176
XmaI CCCGGG 1 cut(s) 77
XmnI GAANNNNTTC 3 cut(s) 266, 369, 957
XspI CTAG 2 cut(s) 284, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.