RLG00000017369

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000429
Physical Location & Seq
Reverse (-)
15789749 .. 15791333
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017369

Sequence Viewer

Length: 1122 bp
ATGGTGACTGGTGAGGTCCATTGTGATTCCATCATATTTCAACACTGTGATCGAGTAAAGATTGATCAAGGTGGAAGCAAAGCAAAGAAAATGGAGAAGCTTGTTCCAAACTGGTACAGTGCTGCTCAACCCTTGCCTGAGACATACATATTCCCACCTGATGCAAGACCAGGGAAGTTTAGCGTTGCTCCTTCAATATCCAACGACATTCCAGTTATTGATCTAGGATCACAGAGTCATGATCGAGCTCTTGTGATCCAGCAAATACTTGAGGCTAGCCAAGAATTCGGTTTCTTTCAGGTAATCAACCATGGCATACCAGAAGCTTTAATGAATGACACAATGGATGTGTTCAAGGAGTTCTTTGAGTTGCCTCTAGAGGAAAAGAGGGCAAGCATCTACTCTGATGACCCCAACAAAGTCTGCAAGCTCGTACATAGCAGTGTTAATTATGATTGGGAGGAAGTTCATCTTTGGCGCGATTTCCTCCGACATCCTTGTGAACCTTTAGAAAAATTCATGCCAATTTGGCCTCGGAAACCGATTAAATATCGAGAGCATGTTAGCAAATGTTTCACCCAAGTGAAGAAAGTGGCTCTGGACATTTTGGAGTTGATCGGTAAAGGACTTGGGATAGGATCAGAATATTTCAATGATGAACTTAGCAAAGAGACTGACATCTTTGTTAATCACTATCCACCTTGTCCTGACCCAAGTTTGACACTTGGAATAACTAAACACTCTGACCCACAGCTCATCACAATTTTACTTCAAGGGGATGTCAGTGGCCTTCAAGTTCTCAAGGATGGGGAATGGATTGGAGTGGAACCTATTTCAAATGGACTGGTGGTTAACATAGGCTATCAATTACGGATCATTAGTAATGGAAAGCTCAAGTGTGCTGAACATCGAGTGGTGACGAATTCAAGTACCGTTCGGACGACGATTGGATTTTTCATTACACCCTCCCCTGACTGCTATATAGAACCTGCAGCAGCTCTTATTAATGCAAGCAATCCCCGACTCTATAAAGGCTTCCGATATGAAGAGTTTCGTGCCAACTACTTTAGGAAGCAGGGGAAAACCGAAGTTGTGCTTGAACCCTTCAAAATCGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

374

Amino Acids

42.33

Weight (kDa)

5.81

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 70 - 178 2.9e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 227 - 322 8.9e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 997
AccII CGCG 1 cut(s) 480
AclWI GGATC 4 cut(s) 235, 250, 646, 881
AcsI RAATTY 3 cut(s) 284, 515, 922
AfaI GTAC 3 cut(s) 116, 435, 931
AjnI CCWGG 1 cut(s) 169
AleI CACNNNNGTG 1 cut(s) 581
AluBI AGCT 7 cut(s) 100, 248, 326, 430, 754, 892, 998
AluI AGCT 7 cut(s) 100, 248, 326, 430, 754, 892, 998
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 134, 665
AlwI GGATC 4 cut(s) 235, 250, 646, 881
AoxI GGCC 2 cut(s) 530, 787
ApeKI GCWGC 3 cut(s) 122, 992, 995
ApoI RAATTY 3 cut(s) 284, 515, 922
AseI ATTAAT 1 cut(s) 1005
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 4 cut(s) 16, 23, 568, 928
AsuNHI GCTAGC 1 cut(s) 275
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 109, 1004, 1007
BccI CCATC 2 cut(s) 38, 800
BciT130I CCWGG 1 cut(s) 171
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 134, 665
BfaI CTAG 3 cut(s) 224, 276, 377
BfmI CTRYAG 1 cut(s) 990
BfuAI ACCTGC 1 cut(s) 997
BglI GCCNNNNNGGC 1 cut(s) 529
BisI GCNGC 3 cut(s) 123, 993, 996
BlsI GCNGC 3 cut(s) 124, 994, 997
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 828
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 2 cut(s) 151, 405
BmtI GCTAGC 1 cut(s) 279
BpuEI CTTGAG 3 cut(s) 290, 785, 878
BsaJI CCNNGG 3 cut(s) 170, 310, 533
Bse1I ACTGG 4 cut(s) 13, 116, 212, 849
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 3 cut(s) 170, 310, 533
BseGI GGATG 4 cut(s) 352, 493, 784, 811
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 4 cut(s) 13, 116, 212, 849
BseXI GCAGC 3 cut(s) 109, 1004, 1007
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 2 cut(s) 532, 789
BsiHKAI GWGCWC 1 cut(s) 250
BsmAI GTCTC 2 cut(s) 134, 665
BsnI GGCC 2 cut(s) 532, 789
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
Bsp19I CCATGG 1 cut(s) 310
BspANI GGCC 2 cut(s) 532, 789
BspCNI CTCAG 1 cut(s) 130
BspFNI CGCG 1 cut(s) 480
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 828
BspMAI CTGCAG 1 cut(s) 994
BspMI ACCTGC 1 cut(s) 997
BspOI GCTAGC 1 cut(s) 279
BspPI GGATC 4 cut(s) 235, 250, 646, 881
BsrI ACTGG 4 cut(s) 13, 116, 212, 849
BssECI CCNNGG 3 cut(s) 170, 310, 533
BssMI GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 47, 119, 934
Bst6I CTCTTC 1 cut(s) 1041
BstC8I GCNNGC 4 cut(s) 277, 394, 428, 1012
BstDEI CTNAG 3 cut(s) 138, 662, 1119
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 4 cut(s) 352, 493, 784, 811
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 9 cut(s) 52, 67, 223, 230, 244, 258, 618, 641, 876
BstMAI GTCTC 2 cut(s) 134, 665
BstMBI GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
BstMWI GCNNNNNNNGC 1 cut(s) 529
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 990
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 109, 1004, 1007
BsuRI GGCC 2 cut(s) 532, 789
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 4 cut(s) 352, 493, 784, 811
BtsI GCAGTG 1 cut(s) 448
BtsIMutI CAGTG 4 cut(s) 43, 124, 448, 790
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 4 cut(s) 277, 394, 428, 1012
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 480
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 115, 434, 930
CviAII CATG 4 cut(s) 239, 311, 520, 560
CviQI GTAC 3 cut(s) 115, 434, 930
DdeI CTNAG 3 cut(s) 138, 662, 1119
DpnI GATC 9 cut(s) 51, 66, 222, 229, 243, 257, 617, 640, 875
DpnII GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
Eam1104I CTCTTC 1 cut(s) 1041
EarI CTCTTC 1 cut(s) 1041
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoRI GAATTC 2 cut(s) 284, 922
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 4 cut(s) 242, 314, 523, 563
FalI AAGNNNNNCTT 6 cut(s) 347, 379, 456, 488, 1080, 1112
FatI CATG 4 cut(s) 238, 310, 519, 559
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 123, 993, 996
FokI GGATG 4 cut(s) 359, 480, 791, 818
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 3 cut(s) 123, 993, 996
FspBI CTAG 3 cut(s) 224, 276, 377
GlaI GCGC 1 cut(s) 479
GluI GCNGC 3 cut(s) 123, 993, 996
HaeIII GGCC 2 cut(s) 532, 789
HhaI GCGC 1 cut(s) 480
Hin1II CATG 4 cut(s) 242, 314, 523, 563
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HindIII AAGCTT 2 cut(s) 98, 324
HinfI GANTC 4 cut(s) 26, 235, 1023, 1115
HpaI GTTAAC 1 cut(s) 853
HphI GGTGA 4 cut(s) 16, 23, 568, 928
Hpy166II GTNNAC 2 cut(s) 503, 853
Hpy188I TCNGA 7 cut(s) 406, 491, 537, 643, 745, 939, 1040
Hpy188III TCNNGA 5 cut(s) 239, 377, 554, 599, 707
Hpy8I GTNNAC 2 cut(s) 503, 853
Hpy99I CGWCG 1 cut(s) 946
HpyAV CCTTC 3 cut(s) 201, 800, 1114
HpyCH4III ACNGT 3 cut(s) 47, 119, 934
HpyCH4V TGCA 4 cut(s) 164, 426, 992, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 529
HpyF3I CTNAG 3 cut(s) 138, 662, 1119
Hsp92II CATG 4 cut(s) 242, 314, 523, 563
HspAI GCGC 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 853
Kzo9I GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 3 cut(s) 109, 1004, 1007
LweI GCATC 2 cut(s) 151, 405
MaeI CTAG 3 cut(s) 224, 276, 377
MaeIII GTNAC 2 cut(s) 4, 916
MalI GATC 9 cut(s) 51, 66, 222, 229, 243, 257, 617, 640, 875
MboI GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
MboII GAAGA 2 cut(s) 598, 1058
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 7 cut(s) 284, 448, 515, 525, 762, 866, 922
MlyI GAGTC 2 cut(s) 244, 1017
MmeI TCCRAC 2 cut(s) 225, 514
MnlI CCTC 9 cut(s) 7, 265, 373, 381, 384, 454, 497, 543, 976
MroXI GAANNNNTTC 1 cut(s) 1050
MseI TTAA 6 cut(s) 329, 447, 546, 687, 852, 1005
MslI CAYNNNNRTG 3 cut(s) 441, 498, 581
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 1 cut(s) 529
NcoI CCATGG 1 cut(s) 310
NdeII GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
NheI GCTAGC 1 cut(s) 275
NlaIII CATG 4 cut(s) 242, 314, 523, 563
NlaIV GGNNCC 1 cut(s) 828
NmuCI GTSAC 2 cut(s) 4, 916
NspI RCATGY 1 cut(s) 563
OliI CACNNNNGTG 1 cut(s) 581
PagI TCATGA 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 2 cut(s) 26, 1115
PkrI GCNGC 3 cut(s) 124, 994, 997
PleI GAGTC 2 cut(s) 243, 1017
PpsI GAGTC 2 cut(s) 243, 1017
PshBI ATTAAT 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 828
PspPI GGNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 994
RsaI GTAC 3 cut(s) 116, 435, 931
RsaNI GTAC 3 cut(s) 115, 434, 930
RseI CAYNNNNRTG 3 cut(s) 441, 498, 581
SacI GAGCTC 1 cut(s) 250
SaqAI TTAA 6 cut(s) 329, 447, 546, 687, 852, 1005
SatI GCNGC 3 cut(s) 123, 993, 996
Sau3AI GATC 9 cut(s) 49, 64, 220, 227, 241, 255, 615, 638, 873
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 2 cut(s) 244, 1017
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 2 cut(s) 151, 405
SfcI CTRYAG 1 cut(s) 990
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 3 cut(s) 441, 498, 581
SmlI CTYRAG 3 cut(s) 269, 800, 893
SmoI CTYRAG 3 cut(s) 269, 800, 893
Sse9I AATT 7 cut(s) 284, 448, 515, 525, 762, 866, 922
SspI AATATT 1 cut(s) 647
SspMI CTAG 3 cut(s) 224, 276, 377
SstI GAGCTC 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 3 cut(s) 47, 119, 934
TaqI TCGA 5 cut(s) 52, 244, 553, 910, 1113
TasI AATT 7 cut(s) 284, 448, 515, 525, 762, 866, 922
TfiI GAWTC 2 cut(s) 26, 1115
Tru1I TTAA 6 cut(s) 329, 447, 546, 687, 852, 1005
Tru9I TTAA 6 cut(s) 329, 447, 546, 687, 852, 1005
TscAI CASTG 4 cut(s) 50, 124, 448, 790
TseFI GTSAC 2 cut(s) 4, 916
TseI GCWGC 3 cut(s) 122, 992, 995
Tsp45I GTSAC 2 cut(s) 4, 916
TspDTI ATGAA 6 cut(s) 347, 458, 508, 672, 946, 1059
TspGWI ACGGA 1 cut(s) 886
TspRI CASTG 4 cut(s) 50, 124, 448, 790
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 1005
XapI RAATTY 3 cut(s) 284, 515, 922
XbaI TCTAGA 1 cut(s) 376
XceI RCATGY 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 3 cut(s) 224, 276, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.