MD10G1188800.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000429
Physical Location & Seq
Forward (+)
28510790 .. 28511617
828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1188800.v1.1.491

Sequence Viewer

Length: 396 bp
ATGTACCTAAACATTGTGATCCAAACCTCAACCATTTTACTTCAAGAAGATCATGTAAATGGACTTCAAGTTTTCAAAAACGGGGAATGGATCAGTGTGGAGCCTATTTCACAAGCATTGGTGGTTAACATTGGCCATCAGCTACAGATTATCAGTAATGGGAAGCTGAAAAGTGTTGAACATTGGGCGGTGACGAGTTCAAGCCATTCCAGGACATCTACTGCATTTTTCATCGCGCCTTCAGACGATTGCATTGTAGAACCTGCGGAAGCTCTTATTAGTGCAAGCAATCCACAGCACTATAAACCCTTTCAATACAAAGAGTTCTTTATCAACTATCTTATGAAGCAGGGTAAAACAGAAGTTTTACTAGAAACGTTTAAGCTCCAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

132

Amino Acids

14.78

Weight (kDa)

5.9

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 9 - 80 3.2e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AccII CGCG 1 cut(s) 236
AciI CCGC 2 cut(s) 188, 266
AclI AACGTT 1 cut(s) 377
AclWI GGATC 2 cut(s) 13, 98
AcoI YGGCCR 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 5
AgsI TTSAA 6 cut(s) 44, 68, 76, 179, 201, 314
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 5 cut(s) 142, 166, 272, 385, 392
AluI AGCT 5 cut(s) 142, 166, 272, 385, 392
AlwI GGATC 2 cut(s) 13, 98
AoxI GGCC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 238
AsuHPI GGTGA 1 cut(s) 202
BalI TGGCCA 1 cut(s) 135
BarI GAAGNNNNNNTAC 2 cut(s) 48, 80
BccI CCATC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 211
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 271
Bme1390I CCNGG 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 1 cut(s) 135
BsnI GGCC 1 cut(s) 135
Bsp143I GATC 3 cut(s) 18, 49, 90
BspACI CCGC 2 cut(s) 188, 266
BspANI GGCC 1 cut(s) 135
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 2 cut(s) 13, 98
BssMI GATC 3 cut(s) 18, 49, 90
Bst2UI CCWGG 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 236
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 3 cut(s) 21, 52, 93
BstMBI GATC 3 cut(s) 18, 49, 90
BstNI CCWGG 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 209
BstSFI CTRYAG 1 cut(s) 143
BstUI CGCG 1 cut(s) 236
BsuRI GGCC 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 286
CfoI GCGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 53
CviJI RGCY 8 cut(s) 103, 135, 142, 166, 204, 272, 385, 392
CviKI_1 RGCY 8 cut(s) 103, 135, 142, 166, 204, 272, 385, 392
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 393
DpnI GATC 3 cut(s) 20, 51, 92
DpnII GATC 3 cut(s) 18, 49, 90
EaeI YGGCCR 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 209
FaeI CATG 1 cut(s) 56
FaiI YATR 3 cut(s) 54, 303, 344
FatI CATG 1 cut(s) 52
FspBI CTAG 1 cut(s) 371
GlaI GCGC 1 cut(s) 237
HaeIII GGCC 1 cut(s) 135
HhaI GCGC 1 cut(s) 238
Hin1II CATG 1 cut(s) 56
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 390
HpaI GTTAAC 1 cut(s) 127
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 1 cut(s) 44
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 1 cut(s) 249
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 3 cut(s) 224, 252, 284
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 1 cut(s) 377
Hsp92II CATG 1 cut(s) 56
HspAI GCGC 1 cut(s) 236
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 18, 49, 90
LmnI GCTCC 2 cut(s) 100, 390
LpnPI CCDG 4 cut(s) 196, 223, 276, 335
MaeI CTAG 1 cut(s) 371
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 1 cut(s) 190
MalI GATC 3 cut(s) 20, 51, 92
MboI GATC 3 cut(s) 18, 49, 90
MboII GAAGA 1 cut(s) 59
MlsI TGGCCA 1 cut(s) 135
MluNI TGGCCA 1 cut(s) 135
MnlI CCTC 1 cut(s) 37
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
MseI TTAA 2 cut(s) 126, 381
MslI CAYNNNNRTG 1 cut(s) 57
Msp20I TGGCCA 1 cut(s) 135
MspR9I CCNGG 1 cut(s) 211
MvaI CCWGG 1 cut(s) 211
MvnI CGCG 1 cut(s) 236
NdeII GATC 3 cut(s) 18, 49, 90
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 190
PfoI TCCNGGA 1 cut(s) 209
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 209
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 102
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 57
SaqAI TTAA 2 cut(s) 126, 381
Sau3AI GATC 3 cut(s) 18, 49, 90
ScrFI CCNGG 1 cut(s) 211
SetI ASST 9 cut(s) 9, 29, 144, 168, 265, 274, 380, 387, 394
SfcI CTRYAG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 57
SsiI CCGC 2 cut(s) 188, 266
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 1 cut(s) 209
TaiI ACGT 1 cut(s) 380
Tru1I TTAA 2 cut(s) 126, 381
Tru9I TTAA 2 cut(s) 126, 381
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 220, 359
TspRI CASTG 1 cut(s) 100
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.