Rh6AG308500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000429
Physical Location & Seq
Forward (+)
50638821 .. 50640671
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG308500.1

Sequence Viewer

Length: 1038 bp
ATGTCTGAGAAGCTCATCTCGAACAGAAGTGACTTGCAATTTGTACCTGAATCCTACATATTACCCCCAGGAACTAGACCAGGCAATACACAGGTCTCTGTATTGGAGGTCATTCCAGTCATTGATTTTCAGCTACTTGGTACCAACAGACCTCAGCTGATCAAGCAAATCATAGAGGCTTCCCAAGAATTTGGCTTCTTCCAGTTGATCAATCACGGGGTTGAAGACAATTTGCTGCATGAGGTATTGGACGTGGCCAACGAGTTATTTGAGCAGCCTTATGAAGACAAGGCAAGCATGTATTCTGAAGATGTTGAGCCAAGTTGTAGACTATACACCAGCATTGATTATAACAAAGAGAAGGTGCATTTTTGGAGAGACAATTTGAGACACCCTTGTCATCCCTTAGAGCAACACATCCATTTCTGGCCTCAGAAACCGCCTCAATATCGGGAGGTGGTTGGAAGTTATTCAGTGGAGGCAAGGAAGCTGAGTATATTGCTTTTGGATCTTATTGGAGAAGGGTTGGGACTTGAATCTGAATTTTTCGGAGATGAACTTACCGAAGTCCAACTGATAGGCATAAACTATTACCCACCTTGTCCAGACCCGAGCTTAACCTTAGGGTTACCAAAACATAGTGATGTTAACCTCATCACTCTTCTTCTCCAAGGAGAGGAAGTTCATGGTCTTCAGGTTTTGAAAGATGGGCAATGGTTGGCTGTTGAGCCTGTCCCTAATGCCTTTGTGGTTAATATAGGCCACACTTTACAGATCATCAGTAATGGGAAGCTAGGCAGCGCCGAGCACAGAGTCATGACAAATGAAAAGGTTTCCCGTACGACAGTGGGTAGCTTCATCCATCCTTCCAGCAATTACCTTGTTGAGCCTGCAAAAGCTCTACTAGATAGGGACTGCAGTAGCTCTCCACTCTTTAGAGCTTTCGTGTACAAGGACTTTGTTAGCACCTACGTAACAAACACTCATGATGGAGTACCACCCCTTGAGCCTCACAAGATCCAGCCACGAAGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

39.0

Weight (kDa)

5.16

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 145 4.4e-23 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 191 - 289 7.1e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AasI GACNNNNNNGTC 1 cut(s) 396
Acc65I GGTACC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 328
AciI CCGC 1 cut(s) 440
AclWI GGATC 2 cut(s) 516, 1012
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 2 cut(s) 188, 542
AcuI CTGAAG 2 cut(s) 327, 677
AfaI GTAC 5 cut(s) 45, 142, 841, 950, 996
AfiI CCNNNNNNNGG 1 cut(s) 676
AgsI TTSAA 3 cut(s) 224, 536, 703
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 2 cut(s) 67, 79
AloI GAACNNNNNNTCC 2 cut(s) 666, 698
Alw21I GWGCWC 1 cut(s) 810
Alw26I GTCTC 3 cut(s) 100, 372, 382
AlwI GGATC 2 cut(s) 516, 1012
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 3 cut(s) 255, 428, 760
ApeKI GCWGC 3 cut(s) 235, 274, 798
ApoI RAATTY 2 cut(s) 188, 542
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
Asp700I GAANNNNTTC 1 cut(s) 469
Asp718I GGTACC 1 cut(s) 140
AspLEI GCGC 1 cut(s) 803
AvaI CYCGRG 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 622
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 140
BbsI GAAGAC 3 cut(s) 231, 291, 683
Bbv12I GWGCWC 1 cut(s) 810
BbvCI CCTCAGC 1 cut(s) 153
BbvI GCAGC 3 cut(s) 222, 286, 810
BccI CCATC 3 cut(s) 701, 870, 983
BciT130I CCWGG 2 cut(s) 69, 81
BclI TGATCA 2 cut(s) 159, 207
BcoDI GTCTC 3 cut(s) 100, 372, 382
BfaI CTAG 3 cut(s) 75, 794, 905
BfmI CTRYAG 1 cut(s) 916
BfoI RGCGCY 1 cut(s) 804
BisI GCNGC 3 cut(s) 236, 275, 799
BlsI GCNGC 3 cut(s) 237, 276, 800
Bme1390I CCNGG 2 cut(s) 69, 81
BmeT110I CYCGRG 1 cut(s) 610
BmgBI CACGTC 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 69, 81
BpiI GAAGAC 3 cut(s) 231, 291, 683
Bpu10I CCTNAGC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 1025
BsaAI YACGTR 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 67, 670
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 116, 202
Bse21I CCTNAGG 1 cut(s) 622
Bse3DI GCAATG 1 cut(s) 719
BseBI CCWGG 2 cut(s) 69, 81
BseDI CCNNGG 2 cut(s) 67, 670
BseGI GGATG 4 cut(s) 400, 417, 858, 862
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 1 cut(s) 719
BseMII CTCAG 3 cut(s) 167, 446, 482
BseNI ACTGG 2 cut(s) 116, 202
BseXI GCAGC 3 cut(s) 222, 286, 810
BshFI GGCC 3 cut(s) 257, 430, 762
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 810
BsiHKCI CYCGRG 1 cut(s) 610
BsiWI CGTACG 1 cut(s) 839
BslFI GGGAC 3 cut(s) 543, 719, 926
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 3 cut(s) 100, 372, 382
BsmFI GGGAC 3 cut(s) 543, 719, 926
BsnI GGCC 3 cut(s) 257, 430, 762
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 610
Bsp1286I GDGCHC 1 cut(s) 810
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 5 cut(s) 159, 207, 508, 774, 1017
BspACI CCGC 1 cut(s) 440
BspANI GGCC 3 cut(s) 257, 430, 762
BspCNI CTCAG 3 cut(s) 166, 445, 483
BspHI TCATGA 2 cut(s) 816, 985
BspLI GGNNCC 1 cut(s) 142
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 2 cut(s) 516, 1012
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 719
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 2 cut(s) 116, 202
BssECI CCNNGG 2 cut(s) 67, 670
BssMI GATC 5 cut(s) 159, 207, 508, 774, 1017
BssT1I CCWWGG 1 cut(s) 670
Bst2UI CCWGG 2 cut(s) 69, 81
Bst4CI ACNGT 1 cut(s) 847
Bst6I CTCTTC 1 cut(s) 666
BstAUI TGTACA 1 cut(s) 948
BstBAI YACGTR 1 cut(s) 973
BstC8I GCNNGC 2 cut(s) 295, 891
BstDEI CTNAG 6 cut(s) 6, 153, 406, 432, 491, 622
BstEII GGTNACC 1 cut(s) 627
BstF5I GGATG 4 cut(s) 400, 417, 858, 862
BstH2I RGCGCY 1 cut(s) 804
BstHHI GCGC 1 cut(s) 803
BstKTI GATC 5 cut(s) 162, 210, 511, 777, 1020
BstMAI GTCTC 3 cut(s) 100, 372, 382
BstMBI GATC 5 cut(s) 159, 207, 508, 774, 1017
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstNI CCWGG 2 cut(s) 69, 81
BstNSI RCATGY 1 cut(s) 301
BstPI GGTNACC 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 67, 79
BstSFI CTRYAG 1 cut(s) 916
BstSNI TACGTA 1 cut(s) 973
BstV1I GCAGC 3 cut(s) 222, 286, 810
BstV2I GAAGAC 3 cut(s) 231, 291, 683
BstX2I RGATCY 2 cut(s) 508, 1017
BstXI CCANNNNNNTGG 1 cut(s) 191
BstYI RGATCY 2 cut(s) 508, 1017
Bsu36I CCTNAGG 1 cut(s) 622
BsuRI GGCC 3 cut(s) 257, 430, 762
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 4 cut(s) 400, 417, 858, 862
BtsIMutI CAGTG 2 cut(s) 480, 852
Cac8I GCNNGC 2 cut(s) 295, 891
CciI TCATGA 2 cut(s) 816, 985
CfoI GCGC 1 cut(s) 803
Csp6I GTAC 5 cut(s) 44, 141, 840, 949, 995
CviAII CATG 5 cut(s) 239, 298, 686, 817, 986
CviQI GTAC 5 cut(s) 44, 141, 840, 949, 995
DdeI CTNAG 6 cut(s) 6, 153, 406, 432, 491, 622
DpnI GATC 5 cut(s) 161, 209, 510, 776, 1019
DpnII GATC 5 cut(s) 159, 207, 508, 774, 1017
DrdI GACNNNNNNGTC 1 cut(s) 396
DseDI GACNNNNNNGTC 1 cut(s) 396
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
Eco105I TACGTA 1 cut(s) 973
Eco130I CCWWGG 1 cut(s) 670
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 327, 677
Eco81I CCTNAGG 1 cut(s) 622
Eco88I CYCGRG 1 cut(s) 610
Eco91I GGTNACC 1 cut(s) 627
EcoO65I GGTNACC 1 cut(s) 627
EcoRII CCWGG 2 cut(s) 67, 79
EcoT14I CCWWGG 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 670
FaeI CATG 5 cut(s) 242, 301, 689, 820, 989
FaqI GGGAC 3 cut(s) 543, 719, 926
FatI CATG 5 cut(s) 238, 297, 685, 816, 985
FbaI TGATCA 2 cut(s) 159, 207
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 3 cut(s) 236, 275, 799
FokI GGATG 4 cut(s) 387, 404, 845, 849
Fsp4HI GCNGC 3 cut(s) 236, 275, 799
FspBI CTAG 3 cut(s) 75, 794, 905
GlaI GCGC 1 cut(s) 802
GluI GCNGC 3 cut(s) 236, 275, 799
HaeII RGCGCY 1 cut(s) 804
HaeIII GGCC 3 cut(s) 257, 430, 762
HhaI GCGC 1 cut(s) 803
Hin1II CATG 5 cut(s) 242, 301, 689, 820, 989
Hin6I GCGC 1 cut(s) 801
HinP1I GCGC 1 cut(s) 801
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 3 cut(s) 50, 536, 813
HpaI GTTAAC 1 cut(s) 649
Hpy166II GTNNAC 3 cut(s) 329, 649, 949
Hpy188I TCNGA 5 cut(s) 7, 307, 435, 541, 551
Hpy188III TCNNGA 5 cut(s) 19, 452, 605, 817, 986
Hpy8I GTNNAC 3 cut(s) 329, 649, 949
HpyAV CCTTC 3 cut(s) 355, 515, 876
HpyCH4III ACNGT 1 cut(s) 847
HpyCH4IV ACGT 2 cut(s) 252, 972
HpyCH4V TGCA 5 cut(s) 37, 238, 367, 893, 918
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 6 cut(s) 6, 153, 406, 432, 491, 622
HpySE526I ACGT 2 cut(s) 252, 972
Hsp92II CATG 5 cut(s) 242, 301, 689, 820, 989
HspAI GCGC 1 cut(s) 801
KpnI GGTACC 1 cut(s) 144
Ksp22I TGATCA 2 cut(s) 159, 207
KspAI GTTAAC 1 cut(s) 649
Kzo9I GATC 5 cut(s) 159, 207, 508, 774, 1017
Lsp1109I GCAGC 3 cut(s) 222, 286, 810
MaeI CTAG 3 cut(s) 75, 794, 905
MaeII ACGT 2 cut(s) 252, 972
MaeIII GTNAC 3 cut(s) 29, 627, 973
MalI GATC 5 cut(s) 161, 209, 510, 776, 1019
MboI GATC 5 cut(s) 159, 207, 508, 774, 1017
MboII GAAGA 7 cut(s) 190, 236, 296, 320, 653, 656, 683
MflI RGATCY 2 cut(s) 508, 1017
MhlI GDGCHC 1 cut(s) 810
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 6 cut(s) 38, 188, 229, 382, 542, 874
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 2 cut(s) 442, 595
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 469
MscI TGGCCA 1 cut(s) 257
MseI TTAA 3 cut(s) 617, 648, 753
MslI CAYNNNNRTG 1 cut(s) 642
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 157
MspR9I CCNGG 2 cut(s) 69, 81
MvaI CCWGG 2 cut(s) 69, 81
MwoI GCNNNNNNNGC 1 cut(s) 163
NdeII GATC 5 cut(s) 159, 207, 508, 774, 1017
NlaIII CATG 5 cut(s) 242, 301, 689, 820, 989
NlaIV GGNNCC 1 cut(s) 142
NmeAIII GCCGAG 1 cut(s) 829
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 301
PagI TCATGA 2 cut(s) 816, 985
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 469
PfeI GAWTC 2 cut(s) 50, 536
Pfl23II CGTACG 1 cut(s) 839
PkrI GCNGC 3 cut(s) 237, 276, 800
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
Ppu21I YACGTR 1 cut(s) 973
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 67, 79
PspEI GGTNACC 1 cut(s) 627
PspGI CCWGG 2 cut(s) 67, 79
PspLI CGTACG 1 cut(s) 839
PspN4I GGNNCC 1 cut(s) 142
PstI CTGCAG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 508, 1017
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 5 cut(s) 45, 142, 841, 950, 996
RsaNI GTAC 5 cut(s) 44, 141, 840, 949, 995
RseI CAYNNNNRTG 1 cut(s) 642
SaqAI TTAA 3 cut(s) 617, 648, 753
SatI GCNGC 3 cut(s) 236, 275, 799
Sau3AI GATC 5 cut(s) 159, 207, 508, 774, 1017
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 2 cut(s) 69, 81
SduI GDGCHC 1 cut(s) 810
SfcI CTRYAG 1 cut(s) 916
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 1004
SmoI CTYRAG 1 cut(s) 1004
SnaBI TACGTA 1 cut(s) 973
Sse9I AATT 6 cut(s) 38, 188, 229, 382, 542, 874
SsiI CCGC 1 cut(s) 440
SspMI CTAG 3 cut(s) 75, 794, 905
StyD4I CCNGG 2 cut(s) 67, 79
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 1 cut(s) 847
TaiI ACGT 2 cut(s) 255, 975
TaqI TCGA 1 cut(s) 20
TasI AATT 6 cut(s) 38, 188, 229, 382, 542, 874
TatI WGTACW 1 cut(s) 948
TfiI GAWTC 2 cut(s) 50, 536
Tru1I TTAA 3 cut(s) 617, 648, 753
Tru9I TTAA 3 cut(s) 617, 648, 753
TscAI CASTG 2 cut(s) 480, 852
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 3 cut(s) 235, 274, 798
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 5 cut(s) 297, 570, 674, 840, 847
TspRI CASTG 2 cut(s) 480, 852
XapI RAATTY 2 cut(s) 188, 542
XceI RCATGY 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 469
XspI CTAG 3 cut(s) 75, 794, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.