MD05G1201900.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000429
Physical Location & Seq
Forward (+)
32934732 .. 32936578
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1201900.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGAGAAGCTTGTTTCAAACTGGTACAACAAGCGAACCTTGCCCGAGAATTACATATTCCCACCAGACGCAAGACCCGGGAAGCTTGCTACCGCTCCTTCGTGCGACAACATTCCAGTGATTGATCTGGGCGGTGATCAAACCTGTATAATTCAGCAAATACTCAAGGCTAGCCACGAATATGGGTTTTTTCAGGTTATCAACCATGGAATATCAGAAAGCTTGTTGAAGGACACAATGGGTGTGTTCAAGGAGTTCTTTGAGTTGCCTGCAGAGGACAAGGCAGCTGTCTACTCTGAGGACCCAAAAAAAATATGTAGACTCTTTACCAGCAGTGGAAATTATGATTGGGAAGATGTTCATCTTTGGCGTGATAACCTACGACACCCTTGTCTTCCTTTAGAGGAGAGCATGAAGCATTGGCCTCAACAGCCAAGTACTTATCGAGAGCTTGTTGGGACATGTTCCTCACAAGTGAGGAAACTGGCTCTGAACATTTTGGAGCTAATCAGTGAAGGACTTGGGCTTGGAACTGGGTATTTTAGAGATGAACTTTTGAAAAATATGTTACTCTTTGTTAATCATTACCCGCCTTGCTCAGACCCAAGTTTGACATTAGGTGTATCTAAGCACTGTGATCCAAACCTCATAACCATTTTACATCAAGAAGAACACGTAAATGGACTTCAAGTTTTCAAAGACGGGGAATGGATTTGTGTGAAGCCTCTTTCTGATGCACTTGTGATTAACATAGGCTATCAGTTACAGATAATCAGTAATGGGAAGCTGAAAAGTGCTGAACATCGGGCAGTGACGAGTTCAAGTAATGCCCGGACATCTGCTGCATTTTTCATCACACCTTCAGACGATTGCATTGTAGAACCTGCTGGAGATCTTATTAATGCAAGCAATCCACAACTCTTTAGATCCTTTCGATACAAAGAGTTCATTATGAACTATCTTACGAAGCAGGGCAAAACTGAAGTTGTACTAGAACCCTTTAAGCTCCATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

339

Amino Acids

38.2

Weight (kDa)

5.78

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 143 2.5e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 188 - 287 1.8e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 390
Acc36I ACCTGC 1 cut(s) 892
AccBSI CCGCTC 1 cut(s) 95
AccI GTMKAC 2 cut(s) 291, 319
AciI CCGC 3 cut(s) 93, 132, 590
AclWI GGATC 2 cut(s) 632, 921
AcuI CTGAAG 2 cut(s) 846, 1002
AfaI GTAC 3 cut(s) 26, 439, 990
AflIII ACRYGT 2 cut(s) 461, 673
AgsI TTSAA 7 cut(s) 18, 229, 250, 559, 689, 697, 822
AjuI GAANNNNNNNTTGG 2 cut(s) 331, 363
AluBI AGCT 8 cut(s) 10, 85, 222, 287, 451, 505, 787, 1006
AluI AGCT 8 cut(s) 10, 85, 222, 287, 451, 505, 787, 1006
AlwI GGATC 2 cut(s) 632, 921
Ama87I CYCGRG 2 cut(s) 44, 77
AoxI GGCC 1 cut(s) 422
ApeKI GCWGC 2 cut(s) 284, 842
AseI ATTAAT 1 cut(s) 900
Asp700I GAANNNNTTC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 301
AsuC2I CCSGG 3 cut(s) 78, 79, 832
AsuHPI GGTGA 1 cut(s) 146
AsuNHI GCTAGC 1 cut(s) 170
AvaI CYCGRG 2 cut(s) 44, 77
AvaII GGWCC 1 cut(s) 301
BarI GAAGNNNNNNTAC 2 cut(s) 669, 701
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 296, 829
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 3 cut(s) 78, 79, 832
BfaI CTAG 2 cut(s) 171, 992
BfmI CTRYAG 1 cut(s) 270
BfuAI ACCTGC 1 cut(s) 892
BglII AGATCT 1 cut(s) 892
BisI GCNGC 2 cut(s) 285, 843
BlsI GCNGC 2 cut(s) 286, 844
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 3 cut(s) 78, 79, 832
Bme18I GGWCC 1 cut(s) 301
BmeT110I CYCGRG 2 cut(s) 44, 77
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 3 cut(s) 78, 79, 832
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 1 cut(s) 724
BmtI GCTAGC 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 3 cut(s) 78, 79, 832
BsaAI YACGTR 1 cut(s) 676
BsaBI GATNNNNATC 1 cut(s) 360
BsaJI CCNNGG 2 cut(s) 77, 205
Bse1I ACTGG 4 cut(s) 26, 116, 489, 538
Bse8I GATNNNNATC 1 cut(s) 360
BseDI CCNNGG 2 cut(s) 77, 205
BseJI GATNNNNATC 1 cut(s) 360
BseMII CTCAG 2 cut(s) 288, 612
BseNI ACTGG 4 cut(s) 26, 116, 489, 538
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 296, 829
BshFI GGCC 1 cut(s) 424
BsiHKCI CYCGRG 2 cut(s) 44, 77
BsiSI CCGG 2 cut(s) 78, 832
BslFI GGGAC 1 cut(s) 472
BsmFI GGGAC 1 cut(s) 472
BsnI GGCC 1 cut(s) 424
BsoBI CYCGRG 2 cut(s) 44, 77
Bsp143I GATC 5 cut(s) 124, 136, 637, 892, 926
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 3 cut(s) 93, 132, 590
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 2 cut(s) 289, 611
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 892
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 632, 921
BsrBI CCGCTC 1 cut(s) 95
BsrI ACTGG 4 cut(s) 26, 116, 489, 538
BssECI CCNNGG 2 cut(s) 77, 205
BssMI GATC 5 cut(s) 124, 136, 637, 892, 926
BssT1I CCWWGG 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 635
BstBAI YACGTR 1 cut(s) 676
BstC8I GCNNGC 4 cut(s) 87, 172, 270, 907
BstDEI CTNAG 4 cut(s) 297, 598, 627, 1014
BstDSI CCRYGG 1 cut(s) 205
BstKTI GATC 5 cut(s) 127, 139, 640, 895, 929
BstMBI GATC 5 cut(s) 124, 136, 637, 892, 926
BstMWI GCNNNNNNNGC 2 cut(s) 40, 430
BstNSI RCATGY 1 cut(s) 465
BstSCI CCNGG 3 cut(s) 76, 77, 830
BstSFI CTRYAG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 296, 829
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 2 cut(s) 892, 926
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 2 cut(s) 892, 926
BsuRI GGCC 1 cut(s) 424
BtgI CCRYGG 1 cut(s) 205
BtsI GCAGTG 2 cut(s) 340, 816
BtsIMutI CAGTG 5 cut(s) 123, 340, 517, 631, 816
BveI ACCTGC 1 cut(s) 892
Cac8I GCNNGC 4 cut(s) 87, 172, 270, 907
Cfr13I GGNCC 1 cut(s) 301
Cfr9I CCCGGG 1 cut(s) 77
CseI GACGC 1 cut(s) 77
Csp6I GTAC 3 cut(s) 25, 438, 989
CviAII CATG 4 cut(s) 206, 412, 462, 1010
CviQI GTAC 3 cut(s) 25, 438, 989
DdeI CTNAG 4 cut(s) 297, 598, 627, 1014
DpnI GATC 5 cut(s) 126, 138, 639, 894, 928
DpnII GATC 5 cut(s) 124, 136, 637, 892, 926
DrdI GACNNNNNNGTC 1 cut(s) 390
DseDI GACNNNNNNGTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 301
Eco57I CTGAAG 2 cut(s) 846, 1002
Eco88I CYCGRG 2 cut(s) 44, 77
EcoO109I RGGNCCY 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 4 cut(s) 209, 415, 465, 1013
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 242, 274
FaqI GGGAC 1 cut(s) 472
FatI CATG 4 cut(s) 205, 411, 461, 1009
FauI CCCGC 1 cut(s) 597
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 2 cut(s) 291, 319
Fnu4HI GCNGC 2 cut(s) 285, 843
Fsp4HI GCNGC 2 cut(s) 285, 843
FspBI CTAG 2 cut(s) 171, 992
GluI GCNGC 2 cut(s) 285, 843
GsuI CTGGAG 1 cut(s) 909
HaeIII GGCC 1 cut(s) 424
HapII CCGG 2 cut(s) 78, 832
HgaI GACGC 1 cut(s) 77
Hin1II CATG 4 cut(s) 209, 415, 465, 1013
HindIII AAGCTT 3 cut(s) 8, 83, 220
HinfI GANTC 1 cut(s) 321
HpaII CCGG 2 cut(s) 78, 832
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 292, 320
Hpy188I TCNGA 6 cut(s) 217, 298, 492, 601, 733, 865
Hpy188III TCNNGA 2 cut(s) 446, 665
Hpy8I GTNNAC 2 cut(s) 292, 320
HpyAV CCTTC 4 cut(s) 108, 223, 509, 870
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 5 cut(s) 272, 737, 845, 873, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 430
HpyF3I CTNAG 4 cut(s) 297, 598, 627, 1014
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 4 cut(s) 209, 415, 465, 1013
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 5 cut(s) 124, 136, 637, 892, 926
LmnI GCTCC 3 cut(s) 100, 502, 1011
Lsp1109I GCAGC 2 cut(s) 296, 829
LweI GCATC 1 cut(s) 724
MaeI CTAG 2 cut(s) 171, 992
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 3 cut(s) 567, 762, 811
MalI GATC 5 cut(s) 126, 138, 639, 894, 928
MbiI CCGCTC 1 cut(s) 95
MboI GATC 5 cut(s) 124, 136, 637, 892, 926
MboII GAAGA 3 cut(s) 365, 386, 680
MflI RGATCY 2 cut(s) 892, 926
MluCI AATT 3 cut(s) 49, 150, 340
MlyI GAGTC 1 cut(s) 315
MnlI CCTC 8 cut(s) 268, 292, 397, 435, 471, 478, 656, 735
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 4 cut(s) 579, 747, 900, 1002
MslI CAYNNNNRTG 3 cut(s) 116, 180, 678
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 78, 832
MspR9I CCNGG 3 cut(s) 78, 79, 832
MwoI GCNNNNNNNGC 2 cut(s) 40, 430
NciI CCSGG 3 cut(s) 78, 79, 832
NcoI CCATGG 1 cut(s) 205
NdeII GATC 5 cut(s) 124, 136, 637, 892, 926
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 4 cut(s) 209, 415, 465, 1013
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 811
NspI RCATGY 1 cut(s) 465
PciI ACATGT 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 357
PkrI GCNGC 2 cut(s) 286, 844
PleI GAGTC 1 cut(s) 315
PpsI GAGTC 1 cut(s) 315
Ppu21I YACGTR 1 cut(s) 676
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 1 cut(s) 301
PspPPI RGGWCCY 1 cut(s) 301
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 2 cut(s) 892, 926
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 3 cut(s) 26, 439, 990
RsaNI GTAC 3 cut(s) 25, 438, 989
RseI CAYNNNNRTG 3 cut(s) 116, 180, 678
SaqAI TTAA 4 cut(s) 579, 747, 900, 1002
SatI GCNGC 2 cut(s) 285, 843
Sau3AI GATC 5 cut(s) 124, 136, 637, 892, 926
Sau96I GGNCC 1 cut(s) 301
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 1 cut(s) 315
ScrFI CCNGG 3 cut(s) 78, 79, 832
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 1 cut(s) 301
SmaI CCCGGG 1 cut(s) 79
SmiMI CAYNNNNRTG 3 cut(s) 116, 180, 678
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 49, 150, 340
SsiI CCGC 3 cut(s) 93, 132, 590
SspMI CTAG 2 cut(s) 171, 992
StyD4I CCNGG 3 cut(s) 76, 77, 830
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 1 cut(s) 678
TaqI TCGA 2 cut(s) 445, 934
TasI AATT 3 cut(s) 49, 150, 340
TatI WGTACW 2 cut(s) 437, 988
Tru1I TTAA 4 cut(s) 579, 747, 900, 1002
Tru9I TTAA 4 cut(s) 579, 747, 900, 1002
TscAI CASTG 5 cut(s) 123, 340, 517, 638, 816
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 2 cut(s) 284, 842
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 6 cut(s) 350, 428, 564, 841, 937, 968
TspMI CCCGGG 1 cut(s) 77
TspRI CASTG 5 cut(s) 123, 340, 517, 638, 816
VpaK11BI GGWCC 1 cut(s) 301
VspI ATTAAT 1 cut(s) 900
XceI RCATGY 1 cut(s) 465
XmaI CCCGGG 1 cut(s) 77
XmiI GTMKAC 2 cut(s) 291, 319
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 2 cut(s) 171, 992
ZrmI AGTACT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.