Rroxscaffold_5G00352210

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000429
Physical Location & Seq
Reverse (-)
29204793 .. 29206297
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352210.1

Sequence Viewer

Length: 810 bp
ATGGCATATCAGAACATTTGTTGCATGACACCATGGATGTGTTCAGGGAGTTTTTTTGCTTTGCCTCCAGAAGACAAGGCAAGCTTCTTCTCTGAGGACCCCAACAAACTCTGCAGGCTCATAACTAGCACTGGAAATTATGACAGGGAGAATGTTCATCTTTGGCGCGATGCCCTCCGACACCCTTGTCACCCTTTAGAGAAATTCATTCCACTTTGGCCTCAACAACCGCTTAAATATCGAGAGCATGTTGGCAAATGTTCCACCCAAGTGAGGAAAGTAGCTTTGAACATTTTAGAGTTGATCAGTGAAGGACTTGGGATTGGATATGGGCACTTCAGCGATGAACTTAGCCAAGACACTCTACTCTCAGTTAATTATTATCCACCTTGTCCAGATCCAAGTTTGACATTAGGAATAACAAAACACTGTGATCCAAACCTCATCACAATTTTACTACAAGGGGATGTATATGGCCTTCAGGTTTTTAAGGATGGGAAATGGATTGGTGTGGAACCTCTTCCGCAAGGACTAGTGGTTAACATAGGCTATCAGTTACAGATCATAAGTAATGGGAAGCTGAAGTGTGCTGAACATCGAGCAGTGACAAATTCAAGTAATGCTCGGACATCTGCTGCATTTTTCATTGCACCTTCCCAAGATTGCCATATACAACCTGCAACAGCTCTTATCAATGCAAGTAATCCTCAAATCTATAAGGGCTTCCAATACAAAGAGTTCCTTTCCAACTACCTAATGAAGCAGGGCAACACCGAAGTTGTACTTGAGCCCTTTAAACTCCAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

269

Amino Acids

30.26

Weight (kDa)

6.69

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 121 - 218 1.5e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 685
AccII CGCG 1 cut(s) 168
AciI CCGC 2 cut(s) 230, 524
AclWI GGATC 2 cut(s) 392, 428
AcsI RAATTY 3 cut(s) 203, 610, 804
AcuI CTGAAG 3 cut(s) 322, 464, 602
AfaI GTAC 1 cut(s) 783
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 2 cut(s) 289, 615
AhlI ACTAGT 1 cut(s) 532
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 4 cut(s) 84, 284, 580, 686
AluI AGCT 4 cut(s) 84, 284, 580, 686
AlwI GGATC 2 cut(s) 392, 428
AoxI GGCC 2 cut(s) 218, 475
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 3 cut(s) 203, 610, 804
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 97
BaeGI GKGCMC 1 cut(s) 336
BanII GRGCYC 1 cut(s) 792
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 622
BccI CCATC 1 cut(s) 488
BclI TGATCA 1 cut(s) 303
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 2 cut(s) 126, 533
BfmI CTRYAG 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 685
BisI GCNGC 1 cut(s) 636
BlsI GCNGC 1 cut(s) 637
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 2 cut(s) 99, 516
BmsI GCATC 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 806
BsaJI CCNNGG 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 3 cut(s) 42, 472, 499
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 84, 384
BseNI ACTGG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 622
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 220, 477
BslI CCNNNNNNNGG 1 cut(s) 273
BsnI GGCC 2 cut(s) 220, 477
Bsp1286I GDGCHC 2 cut(s) 336, 792
Bsp143I GATC 4 cut(s) 303, 397, 433, 561
Bsp19I CCATGG 1 cut(s) 32
BspACI CCGC 2 cut(s) 230, 524
BspANI GGCC 2 cut(s) 220, 477
BspCNI CTCAG 2 cut(s) 85, 383
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 99, 516
BspMAI CTGCAG 1 cut(s) 116
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 2 cut(s) 392, 428
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 4 cut(s) 303, 397, 433, 561
BssT1I CCWWGG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 431
Bst6I CTCTTC 1 cut(s) 525
BstC8I GCNNGC 2 cut(s) 82, 116
BstDEI CTNAG 3 cut(s) 93, 350, 370
BstDSI CCRYGG 1 cut(s) 32
BstF5I GGATG 3 cut(s) 42, 472, 499
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 4 cut(s) 306, 400, 436, 564
BstMBI GATC 4 cut(s) 303, 397, 433, 561
BstNSI RCATGY 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuRI GGCC 2 cut(s) 220, 477
BtgI CCRYGG 1 cut(s) 32
BtgZI GCGATG 2 cut(s) 183, 357
BtsCI GGATG 3 cut(s) 42, 472, 499
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 4 cut(s) 129, 313, 427, 609
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 2 cut(s) 82, 116
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 782
CviAII CATG 3 cut(s) 25, 33, 248
CviQI GTAC 1 cut(s) 782
DdeI CTNAG 3 cut(s) 93, 350, 370
DpnI GATC 4 cut(s) 305, 399, 435, 563
DpnII GATC 4 cut(s) 303, 397, 433, 561
DraI TTTAAA 1 cut(s) 796
DrdI GACNNNNNNGTC 1 cut(s) 186
DseDI GACNNNNNNGTC 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 792
Eco47I GGWCC 1 cut(s) 97
Eco57I CTGAAG 3 cut(s) 322, 464, 602
EcoO109I RGGNCCY 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 32
FaeI CATG 3 cut(s) 28, 36, 251
FalI AAGNNNNNCTT 6 cut(s) 68, 100, 726, 758, 768, 800
FatI CATG 3 cut(s) 24, 32, 247
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 1 cut(s) 636
FokI GGATG 3 cut(s) 49, 479, 506
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 1 cut(s) 636
FspBI CTAG 2 cut(s) 126, 533
GlaI GCGC 1 cut(s) 167
GluI GCNGC 1 cut(s) 636
GsuI CTGGAG 1 cut(s) 51
HaeIII GGCC 2 cut(s) 220, 477
HhaI GCGC 1 cut(s) 168
Hin1II CATG 3 cut(s) 28, 36, 251
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 82
HpaI GTTAAC 1 cut(s) 541
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 4 cut(s) 12, 94, 179, 627
Hpy188III TCNNGA 3 cut(s) 68, 242, 395
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 305, 488, 663
HpyCH4III ACNGT 1 cut(s) 431
HpyCH4V TGCA 6 cut(s) 24, 114, 638, 650, 680, 698
HpyF3I CTNAG 3 cut(s) 93, 350, 370
Hsp92II CATG 3 cut(s) 28, 36, 251
HspAI GCGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 303
KspAI GTTAAC 1 cut(s) 541
Kzo9I GATC 4 cut(s) 303, 397, 433, 561
LpnPI CCDG 9 cut(s) 30, 81, 100, 117, 130, 408, 467, 690, 749
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 1 cut(s) 160
MaeI CTAG 2 cut(s) 126, 533
MaeIII GTNAC 3 cut(s) 188, 555, 604
MalI GATC 4 cut(s) 305, 399, 435, 563
MboI GATC 4 cut(s) 303, 397, 433, 561
MboII GAAGA 3 cut(s) 79, 83, 512
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 2 cut(s) 336, 792
MluCI AATT 6 cut(s) 136, 203, 376, 450, 610, 804
MmeI TCCRAC 2 cut(s) 202, 771
MnlI CCTC 8 cut(s) 75, 88, 185, 231, 267, 452, 528, 717
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 5 cut(s) 234, 375, 489, 540, 795
MslI CAYNNNNRTG 2 cut(s) 37, 269
MvnI CGCG 1 cut(s) 168
NcoI CCATGG 1 cut(s) 32
NdeII GATC 4 cut(s) 303, 397, 433, 561
NlaIII CATG 3 cut(s) 28, 36, 251
NlaIV GGNNCC 2 cut(s) 99, 516
NmuCI GTSAC 2 cut(s) 188, 604
NspI RCATGY 1 cut(s) 251
OliI CACNNNNGTG 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 519
PkrI GCNGC 1 cut(s) 637
PpuMI RGGWCCY 1 cut(s) 97
Psp5II RGGWCCY 1 cut(s) 97
PspN4I GGNNCC 2 cut(s) 99, 516
PspPI GGNCC 1 cut(s) 97
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 397
RsaI GTAC 1 cut(s) 783
RsaNI GTAC 1 cut(s) 782
RseI CAYNNNNRTG 2 cut(s) 37, 269
SaqAI TTAA 5 cut(s) 234, 375, 489, 540, 795
SatI GCNGC 1 cut(s) 636
Sau3AI GATC 4 cut(s) 303, 397, 433, 561
Sau96I GGNCC 1 cut(s) 97
SduI GDGCHC 2 cut(s) 336, 792
SfaNI GCATC 1 cut(s) 160
SfcI CTRYAG 1 cut(s) 112
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 37, 269
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
SpeI ACTAGT 1 cut(s) 532
Sse9I AATT 6 cut(s) 136, 203, 376, 450, 610, 804
SsiI CCGC 2 cut(s) 230, 524
SspMI CTAG 2 cut(s) 126, 533
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 1 cut(s) 431
TaqI TCGA 2 cut(s) 241, 598
TasI AATT 6 cut(s) 136, 203, 376, 450, 610, 804
TatI WGTACW 1 cut(s) 781
Tru1I TTAA 5 cut(s) 234, 375, 489, 540, 795
Tru9I TTAA 5 cut(s) 234, 375, 489, 540, 795
TscAI CASTG 4 cut(s) 136, 313, 434, 609
TseFI GTSAC 2 cut(s) 188, 604
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 2 cut(s) 188, 604
TspDTI ATGAA 5 cut(s) 146, 196, 360, 634, 773
TspRI CASTG 4 cut(s) 136, 313, 434, 609
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 3 cut(s) 203, 610, 804
XceI RCATGY 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 126, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.