Rw4G011820

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000429
Physical Location & Seq
Reverse (-)
27965868 .. 27967198
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011820.1

Sequence Viewer

Length: 1035 bp
ATGGAGAAGCTTGTTTCTAGCTGGTACAATAATGTTCAACCCTTGCCCGAGACTTACATATTCCCACCCGATGCAAGACCGGGGAAGCTTAGCACTCTTCCTTCATCTGACAACATTCCAGTGATTAATCTTGGTGGTGGTGGAGCAGAAGGTCATAATCGAACTCGTATAATTGAGCAAATACTTGGGGCTAGCCAAGAGTTCGGGTTTTTTCAAGTAATCAACCATGGCATATCAGAAAATTTGTTGCATGACACCATGGATGTGTTCAGGGAGTTTTTTGCTTTGCCTCCAGAAGACAAGGCAAGCTTCTTCTCTGAGGACCCCAACAAACTCTGCAGGCTCATAACTAGCACCGGAAATTATGACAGGGAGAATGTTCATCTTTGGCGCGATGCCCTCCGACACCCTTGTCACCCTTTAGAAAAATTCATTCCACTTTGGCCTCAACAACCGCTTAAATATCGAGAGCATGTTGGCAAATGTTCCACCCAAGTGAGGAAAGTAGCTTTGAACATTTTAGACTTGATCAGTGAAGGACTTGGGATTGGATATGGGCACTTCAGCGATGAACTTAGCCAAGAGACTCTACTCTCAGTTAATTATTATCCACCTTGTCCAGATCCAAGTTTGACATTAGGAATAACTAAACACTGTGATCCAAACCTCATCACAATTTTACTTCAAGGGGATGTACATGGCCTTCAGGTTTTTAAGGATGGGAAATGGATTGGTGTGGAACCTCTTCCACAAGGACTAGTGGTTAACATAGGCTATCAGTTACAGATCATAAGTAATGGGAAGCTGAAGTGTGCTGAACATCGAGCAGTGACAAATTCAAGTAATGCTCGGACATCTGCTGCATTTTTCATTGCACCTTCCCAAGATTGCAATATACAACCTGCAACAGCTCTTATCAATGCAAGTAATCCTCAAATCTATAAGGGCTTCCAATACAAAGAGTTCCTTTCCAACTACCTAATGAAGCAGGGCAACACCGAAGTTGTACTTGAGCCCTTTAAACTCCAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

344

Amino Acids

38.51

Weight (kDa)

6.0

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 39 - 150 4e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 197 - 293 1.7e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 411
Acc36I ACCTGC 1 cut(s) 910
AccII CGCG 1 cut(s) 393
AciI CCGC 1 cut(s) 455
AclWI GGATC 2 cut(s) 617, 653
AcsI RAATTY 4 cut(s) 241, 428, 835, 1029
AcuI CTGAAG 3 cut(s) 547, 689, 827
AfaI GTAC 3 cut(s) 26, 696, 1008
AfiI CCNNNNNNNGG 1 cut(s) 498
AgsI TTSAA 5 cut(s) 38, 215, 514, 686, 840
AhlI ACTAGT 1 cut(s) 757
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 7 cut(s) 10, 21, 88, 309, 509, 805, 911
AluI AGCT 7 cut(s) 10, 21, 88, 309, 509, 805, 911
Alw26I GTCTC 2 cut(s) 44, 578
AlwI GGATC 2 cut(s) 617, 653
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 2 cut(s) 443, 700
ApeKI GCWGC 1 cut(s) 860
ApoI RAATTY 4 cut(s) 241, 428, 835, 1029
AseI ATTAAT 1 cut(s) 126
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 407
AsuNHI GCTAGC 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 561
BanII GRGCYC 1 cut(s) 1017
BarI GAAGNNNNNNTAC 2 cut(s) 687, 719
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 847
BccI CCATC 1 cut(s) 713
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 44, 578
BcuI ACTAGT 1 cut(s) 757
BfaI CTAG 4 cut(s) 18, 192, 351, 758
BfmI CTRYAG 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 910
BisI GCNGC 1 cut(s) 861
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 1 cut(s) 862
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 324, 741
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 61, 385
BmtI GCTAGC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 276
Bpu1102I GCTNAGC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 1031
BpuMI CCSGG 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 80, 226, 258
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 870
BseDI CCNNGG 3 cut(s) 80, 226, 258
BseGI GGATG 3 cut(s) 268, 697, 724
BseLI CCNNNNNNNGG 1 cut(s) 498
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 2 cut(s) 309, 609
BseNI ACTGG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 1 cut(s) 847
Bsh1236I CGCG 1 cut(s) 393
BshFI GGCC 2 cut(s) 445, 702
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 80, 357
BslI CCNNNNNNNGG 1 cut(s) 498
BsmAI GTCTC 2 cut(s) 44, 578
BsnI GGCC 2 cut(s) 445, 702
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 561, 1017
Bsp1407I TGTACA 1 cut(s) 694
Bsp143I GATC 4 cut(s) 528, 622, 658, 786
Bsp1720I GCTNAGC 1 cut(s) 89
Bsp19I CCATGG 2 cut(s) 226, 258
BspACI CCGC 1 cut(s) 455
BspANI GGCC 2 cut(s) 445, 702
BspCNI CTCAG 2 cut(s) 310, 608
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 324, 741
BspMAI CTGCAG 1 cut(s) 341
BspMI ACCTGC 1 cut(s) 910
BspOI GCTAGC 1 cut(s) 195
BspPI GGATC 2 cut(s) 617, 653
BsrDI GCAATG 1 cut(s) 870
BsrGI TGTACA 1 cut(s) 694
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 80, 226, 258
BssMI GATC 4 cut(s) 528, 622, 658, 786
BssT1I CCWWGG 2 cut(s) 226, 258
Bst4CI ACNGT 1 cut(s) 656
Bst6I CTCTTC 2 cut(s) 102, 750
BstAUI TGTACA 1 cut(s) 694
BstC8I GCNNGC 3 cut(s) 193, 307, 341
BstDEI CTNAG 4 cut(s) 89, 318, 575, 595
BstDSI CCRYGG 2 cut(s) 226, 258
BstF5I GGATG 3 cut(s) 268, 697, 724
BstFNI CGCG 1 cut(s) 393
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 4 cut(s) 531, 625, 661, 789
BstMAI GTCTC 2 cut(s) 44, 578
BstMBI GATC 4 cut(s) 528, 622, 658, 786
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 847
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BsuRI GGCC 2 cut(s) 445, 702
BtgI CCRYGG 2 cut(s) 226, 258
BtgZI GCGATG 2 cut(s) 408, 582
BtsCI GGATG 3 cut(s) 268, 697, 724
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 4 cut(s) 126, 538, 652, 834
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 3 cut(s) 193, 307, 341
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 3 cut(s) 25, 695, 1007
CviAII CATG 5 cut(s) 227, 251, 259, 473, 698
CviQI GTAC 3 cut(s) 25, 695, 1007
DdeI CTNAG 4 cut(s) 89, 318, 575, 595
DpnI GATC 4 cut(s) 530, 624, 660, 788
DpnII GATC 4 cut(s) 528, 622, 658, 786
DraI TTTAAA 1 cut(s) 1021
DrdI GACNNNNNNGTC 1 cut(s) 411
DseDI GACNNNNNNGTC 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 102, 750
EarI CTCTTC 2 cut(s) 102, 750
Eco130I CCWWGG 2 cut(s) 226, 258
Eco24I GRGCYC 1 cut(s) 1017
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 3 cut(s) 547, 689, 827
Eco88I CYCGRG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 226, 258
EcoT38I GRGCYC 1 cut(s) 1017
ErhI CCWWGG 2 cut(s) 226, 258
FaeI CATG 5 cut(s) 230, 254, 262, 476, 701
FalI AAGNNNNNCTT 6 cut(s) 293, 325, 951, 983, 993, 1025
FatI CATG 5 cut(s) 226, 250, 258, 472, 697
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 1 cut(s) 861
FokI GGATG 3 cut(s) 275, 704, 731
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 1 cut(s) 861
FspBI CTAG 4 cut(s) 18, 192, 351, 758
GlaI GCGC 1 cut(s) 392
GluI GCNGC 1 cut(s) 861
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 2 cut(s) 445, 702
HapII CCGG 2 cut(s) 80, 357
HhaI GCGC 1 cut(s) 393
Hin1II CATG 5 cut(s) 230, 254, 262, 476, 701
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 3 cut(s) 8, 86, 307
HinfI GANTC 1 cut(s) 586
HpaI GTTAAC 1 cut(s) 766
HpaII CCGG 2 cut(s) 80, 357
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 1 cut(s) 766
Hpy188I TCNGA 5 cut(s) 109, 238, 319, 404, 852
Hpy188III TCNNGA 3 cut(s) 293, 467, 620
Hpy8I GTNNAC 1 cut(s) 766
HpyAV CCTTC 5 cut(s) 111, 143, 530, 713, 888
HpyCH4III ACNGT 1 cut(s) 656
HpyCH4V TGCA 8 cut(s) 74, 250, 339, 863, 875, 891, 905, 923
HpyF3I CTNAG 4 cut(s) 89, 318, 575, 595
Hsp92II CATG 5 cut(s) 230, 254, 262, 476, 701
HspAI GCGC 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 528
KspAI GTTAAC 1 cut(s) 766
Kzo9I GATC 4 cut(s) 528, 622, 658, 786
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 1 cut(s) 847
LweI GCATC 2 cut(s) 61, 385
MaeI CTAG 4 cut(s) 18, 192, 351, 758
MaeIII GTNAC 3 cut(s) 413, 780, 829
MalI GATC 4 cut(s) 530, 624, 660, 788
MboI GATC 4 cut(s) 528, 622, 658, 786
MboII GAAGA 4 cut(s) 89, 304, 308, 737
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 2 cut(s) 561, 1017
MluCI AATT 8 cut(s) 171, 241, 361, 428, 601, 675, 835, 1029
MlyI GAGTC 1 cut(s) 580
MmeI TCCRAC 2 cut(s) 427, 996
MnlI CCTC 8 cut(s) 300, 313, 410, 456, 492, 677, 753, 942
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 6 cut(s) 126, 459, 600, 714, 765, 1020
MslI CAYNNNNRTG 3 cut(s) 119, 263, 494
MspI CCGG 2 cut(s) 80, 357
MspR9I CCNGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 393
NciI CCSGG 1 cut(s) 81
NcoI CCATGG 2 cut(s) 226, 258
NdeII GATC 4 cut(s) 528, 622, 658, 786
NheI GCTAGC 1 cut(s) 191
NlaIII CATG 5 cut(s) 230, 254, 262, 476, 701
NlaIV GGNNCC 2 cut(s) 324, 741
NmuCI GTSAC 2 cut(s) 413, 829
NspI RCATGY 1 cut(s) 476
OliI CACNNNNGTG 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 744
PkrI GCNGC 1 cut(s) 862
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PpuMI RGGWCCY 1 cut(s) 322
PshBI ATTAAT 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 324, 741
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PstI CTGCAG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 622
RsaI GTAC 3 cut(s) 26, 696, 1008
RsaNI GTAC 3 cut(s) 25, 695, 1007
RseI CAYNNNNRTG 3 cut(s) 119, 263, 494
SaqAI TTAA 6 cut(s) 126, 459, 600, 714, 765, 1020
SatI GCNGC 1 cut(s) 861
Sau3AI GATC 4 cut(s) 528, 622, 658, 786
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 1 cut(s) 580
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 2 cut(s) 561, 1017
SfaNI GCATC 2 cut(s) 61, 385
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 3 cut(s) 119, 263, 494
SmlI CTYRAG 1 cut(s) 1010
SmoI CTYRAG 1 cut(s) 1010
SpeI ACTAGT 1 cut(s) 757
Sse9I AATT 8 cut(s) 171, 241, 361, 428, 601, 675, 835, 1029
SsiI CCGC 1 cut(s) 455
SspMI CTAG 4 cut(s) 18, 192, 351, 758
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 2 cut(s) 226, 258
TaaI ACNGT 1 cut(s) 656
TaqI TCGA 3 cut(s) 160, 466, 823
TasI AATT 8 cut(s) 171, 241, 361, 428, 601, 675, 835, 1029
TatI WGTACW 2 cut(s) 694, 1006
Tru1I TTAA 6 cut(s) 126, 459, 600, 714, 765, 1020
Tru9I TTAA 6 cut(s) 126, 459, 600, 714, 765, 1020
TscAI CASTG 4 cut(s) 126, 538, 659, 834
TseFI GTSAC 2 cut(s) 413, 829
TseI GCWGC 1 cut(s) 860
Tsp45I GTSAC 2 cut(s) 413, 829
TspDTI ATGAA 6 cut(s) 93, 371, 421, 585, 859, 998
TspRI CASTG 4 cut(s) 126, 538, 659, 834
VpaK11BI GGWCC 1 cut(s) 322
VspI ATTAAT 1 cut(s) 126
XapI RAATTY 4 cut(s) 241, 428, 835, 1029
XceI RCATGY 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 4 cut(s) 18, 192, 351, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.