pycom05g18530

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000429
Physical Location & Seq
Reverse (-)
21466019 .. 21467474
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18530.1

Sequence Viewer

Length: 1023 bp
ATGGAGAAGCTTGTTTCAAGCTGGTACAAGGATGGAACCTTGCCGGAGAGTTTCATATGGCCACCGGATTCAAGACCCGGGAAGCTTGCTGCTGCTCCTTCATGCAAGAACATTCCAGTTATTGATCTTGGTGGAGCTGAAGGCGGTGATCGTGCCTACATCATTCACCAAATACTCGAGGCCGGTCAAGAATTCGGATTTTTTCAGGCAATCAACCATGGCATCTCAGATACTCTGTTAAGTGACACAATGAGTGTGTTGAAGGAGTTCTTTGAGTTGCCGCTAGAGGACAAGGCAAGCGTTTACTCTGAGGACCCCCAAAAAAGTTGCAAGCTCTTCAGCAGCAGCATAAATTATGATGGGGAAGATGTTCATCTTTGGCGCGATGTCCTACGACACCCTTGTCATCCATTGGTGGAATGCTTGCAAGATTGGCCTCAGCAGCCAGAGCTTGTTGGGACATGTTCTACACAAGTGAGGAAACTGGCTCTGAACATTTTGGAGCTAATCAGTGAAGGACTTGGGCTCGGAACTGGGTACTTCAGAAACGAACTTAGCCGAAACTTGATACTCTCAGTTAATCATTACCCGCCTTGCCCCGACCCAAGTTTGACATTAGGATTAACTAAACATTGTGACCCAAACGTCATAACCATCTTAATGCAAGAGGATCATGTAAATGGACTTCAAGTTTTCAAAGACGGGGAATGGGTTGGTGTGGAGCCTATTTCACATGCACTTGTGGTTAACATAGGCTATCAGTTACAGATCATCAGTAATGGGAAGCTGAAAAGTTCTGAACATCGGGCTGTGACGAATCCATGTAATGCTAGGACATCTGCAGGATTTTTCGTTGCGCCTTCTGATGGTTGCGTTGTAGAACCTGCAAGAGCTCTTGTTGGTGCAAGCAATCCCCAACTATATAAAAGTTCCCAGTACAAAGAGTTTGTTCGCAACTTTAATACGACGATATTCGAAGGTAAAAGCGAATTTGCAATGGAAAGCCTTAAGCTCCAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

341

Amino Acids

37.64

Weight (kDa)

5.23

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 38 - 147 7.3e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 189 - 287 2.9e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 402, 644
Acc36I ACCTGC 1 cut(s) 892
AccII CGCG 1 cut(s) 384
AciI CCGC 3 cut(s) 144, 281, 590
AclWI GGATC 1 cut(s) 678
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 191, 989
AcuI CTGAAG 3 cut(s) 159, 322, 526
AfaI GTAC 3 cut(s) 26, 539, 938
AfiI CCNNNNNNNGG 1 cut(s) 866
AflII CTTAAG 1 cut(s) 1007
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 5 cut(s) 18, 72, 262, 689, 697
Alw21I GWGCWC 1 cut(s) 895
AlwI GGATC 1 cut(s) 678
Ama87I CYCGRG 2 cut(s) 77, 176
AoxI GGCC 3 cut(s) 59, 180, 434
ApeKI GCWGC 5 cut(s) 89, 92, 342, 345, 442
ApoI RAATTY 2 cut(s) 191, 989
Asp700I GAANNNNTTC 2 cut(s) 266, 369
AspLEI GCGC 2 cut(s) 384, 859
AspS9I GGNCC 1 cut(s) 313
AsuC2I CCSGG 2 cut(s) 78, 79
AsuHPI GGTGA 2 cut(s) 158, 158
AsuII TTCGAA 1 cut(s) 975
AvaI CYCGRG 2 cut(s) 77, 176
AvaII GGWCC 1 cut(s) 313
BalI TGGCCA 1 cut(s) 61
BanII GRGCYC 2 cut(s) 528, 895
BarI GAAGNNNNNNTAC 2 cut(s) 669, 701
Bbv12I GWGCWC 1 cut(s) 895
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 5 cut(s) 76, 79, 354, 357, 454
BccI CCATC 4 cut(s) 26, 353, 662, 860
BcnI CCSGG 2 cut(s) 78, 79
BfaI CTAG 2 cut(s) 284, 831
BfmI CTRYAG 1 cut(s) 840
BfrI CTTAAG 1 cut(s) 1007
BfuAI ACCTGC 1 cut(s) 892
BisI GCNGC 6 cut(s) 90, 93, 281, 343, 346, 443
BlsI GCNGC 6 cut(s) 91, 94, 282, 344, 347, 444
Bme1390I CCNGG 2 cut(s) 78, 79
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 2 cut(s) 77, 176
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 3 cut(s) 37, 315, 723
BmrFI CCNGG 2 cut(s) 78, 79
BmrI ACTGGG 2 cut(s) 543, 928
BmsI GCATC 1 cut(s) 231
BmuI ACTGGG 2 cut(s) 543, 928
Bpu10I CCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 975
BpuMI CCSGG 2 cut(s) 78, 79
BsaBI GATNNNNATC 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 77, 217
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 4 cut(s) 116, 489, 538, 934
Bse3DI GCAATG 1 cut(s) 1002
Bse8I GATNNNNATC 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 77, 217
BseGI GGATG 2 cut(s) 37, 406
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMI GCAATG 1 cut(s) 1002
BseMII CTCAG 4 cut(s) 240, 300, 452, 588
BseNI ACTGG 4 cut(s) 116, 489, 538, 934
BseXI GCAGC 5 cut(s) 76, 79, 354, 357, 454
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 3 cut(s) 61, 182, 436
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 2 cut(s) 77, 176
BsiSI CCGG 4 cut(s) 44, 65, 78, 183
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 1 cut(s) 866
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 3 cut(s) 61, 182, 436
BsoBI CYCGRG 2 cut(s) 77, 176
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 2 cut(s) 528, 895
Bsp143I GATC 4 cut(s) 124, 148, 670, 768
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 3 cut(s) 144, 281, 590
BspANI GGCC 3 cut(s) 61, 182, 436
BspCNI CTCAG 4 cut(s) 239, 301, 451, 587
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 3 cut(s) 37, 315, 723
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 892
BspPI GGATC 1 cut(s) 678
BspQI GCTCTTC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 975
BspTI CTTAAG 1 cut(s) 1007
BsrDI GCAATG 1 cut(s) 1002
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 4 cut(s) 116, 489, 538, 934
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 77, 217
BssMI GATC 4 cut(s) 124, 148, 670, 768
BssT1I CCWWGG 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 341
BstAFI CTTAAG 1 cut(s) 1007
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 5 cut(s) 87, 298, 332, 425, 907
BstDEI CTNAG 6 cut(s) 226, 309, 438, 554, 574, 1020
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 2 cut(s) 37, 406
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 2 cut(s) 384, 859
BstKTI GATC 4 cut(s) 127, 151, 673, 771
BstMBI GATC 4 cut(s) 124, 148, 670, 768
BstMWI GCNNNNNNNGC 3 cut(s) 433, 442, 448
BstNSI RCATGY 2 cut(s) 465, 737
BstSCI CCNGG 2 cut(s) 76, 77
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 5 cut(s) 76, 79, 354, 357, 454
BsuRI GGCC 3 cut(s) 61, 182, 436
BtgI CCRYGG 1 cut(s) 217
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 2 cut(s) 37, 406
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 1 cut(s) 892
Cac8I GCNNGC 5 cut(s) 87, 298, 332, 425, 907
CfoI GCGC 2 cut(s) 384, 859
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 313
Cfr9I CCCGGG 1 cut(s) 77
Csp6I GTAC 3 cut(s) 25, 538, 937
CviAII CATG 6 cut(s) 102, 218, 462, 674, 734, 822
CviQI GTAC 3 cut(s) 25, 538, 937
DdeI CTNAG 6 cut(s) 226, 309, 438, 554, 574, 1020
DpnI GATC 4 cut(s) 126, 150, 672, 770
DpnII GATC 4 cut(s) 124, 148, 670, 768
DrdI GACNNNNNNGTC 2 cut(s) 402, 644
DseDI GACNNNNNNGTC 2 cut(s) 402, 644
EaeI YGGCCR 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 893
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 2 cut(s) 528, 895
Eco47I GGWCC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 893
Eco57I CTGAAG 3 cut(s) 159, 322, 526
Eco88I CYCGRG 2 cut(s) 77, 176
EcoICRI GAGCTC 1 cut(s) 893
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 2 cut(s) 528, 895
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 6 cut(s) 105, 221, 465, 677, 737, 825
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 472
FatI CATG 6 cut(s) 101, 217, 461, 673, 733, 821
FauI CCCGC 1 cut(s) 597
FauNDI CATATG 1 cut(s) 56
Fnu4HI GCNGC 6 cut(s) 90, 93, 281, 343, 346, 443
FokI GGATG 2 cut(s) 44, 393
FriOI GRGCYC 2 cut(s) 528, 895
Fsp4HI GCNGC 6 cut(s) 90, 93, 281, 343, 346, 443
FspBI CTAG 2 cut(s) 284, 831
GlaI GCGC 2 cut(s) 383, 858
GluI GCNGC 6 cut(s) 90, 93, 281, 343, 346, 443
HaeIII GGCC 3 cut(s) 61, 182, 436
HapII CCGG 4 cut(s) 44, 65, 78, 183
HhaI GCGC 2 cut(s) 384, 859
Hin1II CATG 6 cut(s) 105, 221, 465, 677, 737, 825
Hin6I GCGC 2 cut(s) 382, 857
HinP1I GCGC 2 cut(s) 382, 857
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 2 cut(s) 8, 83
HinfI GANTC 2 cut(s) 68, 817
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 4 cut(s) 44, 65, 78, 183
HphI GGTGA 2 cut(s) 158, 158
Hpy166II GTNNAC 2 cut(s) 304, 748
Hpy188I TCNGA 8 cut(s) 197, 229, 310, 492, 530, 545, 799, 865
Hpy188III TCNNGA 2 cut(s) 72, 188
Hpy8I GTNNAC 2 cut(s) 304, 748
Hpy99I CGWCG 1 cut(s) 970
HpyAV CCTTC 6 cut(s) 108, 134, 256, 509, 870, 971
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 9 cut(s) 105, 330, 427, 664, 737, 842, 887, 905, 995
HpyF10VI GCNNNNNNNGC 3 cut(s) 433, 442, 448
HpyF3I CTNAG 6 cut(s) 226, 309, 438, 554, 574, 1020
HpySE526I ACGT 1 cut(s) 645
Hsp92II CATG 6 cut(s) 105, 221, 465, 677, 737, 825
HspAI GCGC 2 cut(s) 382, 857
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 4 cut(s) 124, 148, 670, 768
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 5 cut(s) 100, 134, 502, 721, 1017
Lsp1109I GCAGC 5 cut(s) 76, 79, 354, 357, 454
LweI GCATC 1 cut(s) 231
MaeI CTAG 2 cut(s) 284, 831
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 4 cut(s) 242, 635, 762, 811
MalI GATC 4 cut(s) 126, 150, 672, 770
MboI GATC 4 cut(s) 124, 148, 670, 768
MboII GAAGA 2 cut(s) 328, 377
MhlI GDGCHC 2 cut(s) 528, 895
MlsI TGGCCA 1 cut(s) 61
MluCI AATT 3 cut(s) 191, 352, 989
MluNI TGGCCA 1 cut(s) 61
MnlI CCTC 6 cut(s) 172, 280, 304, 447, 471, 661
Mox20I TGGCCA 1 cut(s) 61
MroXI GAANNNNTTC 2 cut(s) 266, 369
MscI TGGCCA 1 cut(s) 61
MseI TTAA 7 cut(s) 239, 579, 623, 659, 747, 960, 1008
MslI CAYNNNNRTG 2 cut(s) 659, 678
Msp20I TGGCCA 1 cut(s) 61
MspCI CTTAAG 1 cut(s) 1007
MspI CCGG 4 cut(s) 44, 65, 78, 183
MspR9I CCNGG 2 cut(s) 78, 79
Mva1269I GAATGC 1 cut(s) 425
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 3 cut(s) 433, 442, 448
NciI CCSGG 2 cut(s) 78, 79
NcoI CCATGG 1 cut(s) 217
NdeI CATATG 1 cut(s) 56
NdeII GATC 4 cut(s) 124, 148, 670, 768
NlaIII CATG 6 cut(s) 105, 221, 465, 677, 737, 825
NlaIV GGNNCC 3 cut(s) 37, 315, 723
NmuCI GTSAC 3 cut(s) 242, 635, 811
NspI RCATGY 2 cut(s) 465, 737
NspV TTCGAA 1 cut(s) 975
PaeR7I CTCGAG 1 cut(s) 176
PciI ACATGT 1 cut(s) 461
PciSI GCTCTTC 1 cut(s) 341
PctI GAATGC 1 cut(s) 425
PdmI GAANNNNTTC 2 cut(s) 266, 369
PfeI GAWTC 2 cut(s) 68, 817
PkrI GCNGC 6 cut(s) 91, 94, 282, 344, 347, 444
PpuMI RGGWCCY 1 cut(s) 313
PscI ACATGT 1 cut(s) 461
Psp124BI GAGCTC 1 cut(s) 895
Psp5II RGGWCCY 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 37, 315, 723
PspPI GGNCC 1 cut(s) 313
PspPPI RGGWCCY 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 1 cut(s) 844
RsaI GTAC 3 cut(s) 26, 539, 938
RsaNI GTAC 3 cut(s) 25, 538, 937
RseI CAYNNNNRTG 2 cut(s) 659, 678
SacI GAGCTC 1 cut(s) 895
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 7 cut(s) 239, 579, 623, 659, 747, 960, 1008
SatI GCNGC 6 cut(s) 90, 93, 281, 343, 346, 443
Sau3AI GATC 4 cut(s) 124, 148, 670, 768
Sau96I GGNCC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 78, 79
SduI GDGCHC 2 cut(s) 528, 895
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 975
SinI GGWCC 1 cut(s) 313
SlaI CTCGAG 1 cut(s) 176
SmaI CCCGGG 1 cut(s) 79
SmiMI CAYNNNNRTG 2 cut(s) 659, 678
SmlI CTYRAG 2 cut(s) 176, 1007
SmoI CTYRAG 2 cut(s) 176, 1007
Sse9I AATT 3 cut(s) 191, 352, 989
SsiI CCGC 3 cut(s) 144, 281, 590
SspMI CTAG 2 cut(s) 284, 831
SstI GAGCTC 1 cut(s) 895
StyD4I CCNGG 2 cut(s) 76, 77
StyI CCWWGG 1 cut(s) 217
TaiI ACGT 1 cut(s) 648
TaqI TCGA 2 cut(s) 177, 975
TasI AATT 3 cut(s) 191, 352, 989
TatI WGTACW 1 cut(s) 936
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 2 cut(s) 68, 817
Tru1I TTAA 7 cut(s) 239, 579, 623, 659, 747, 960, 1008
Tru9I TTAA 7 cut(s) 239, 579, 623, 659, 747, 960, 1008
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 3 cut(s) 242, 635, 811
TseI GCWGC 5 cut(s) 89, 92, 342, 345, 442
Tsp45I GTSAC 3 cut(s) 242, 635, 811
TspDTI ATGAA 3 cut(s) 43, 90, 362
TspMI CCCGGG 1 cut(s) 77
TspRI CASTG 1 cut(s) 517
Vha464I CTTAAG 1 cut(s) 1007
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 2 cut(s) 191, 989
XceI RCATGY 2 cut(s) 465, 737
XhoI CTCGAG 1 cut(s) 176
XmaI CCCGGG 1 cut(s) 77
XmnI GAANNNNTTC 2 cut(s) 266, 369
XspI CTAG 2 cut(s) 284, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.