Rh6DG307100

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000429
Physical Location & Seq
Forward (+)
50778458 .. 50778911
454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG307100.1

Sequence Viewer

Length: 300 bp
ATGAATGTTGAGCCTATCCCTAATGCACATGTGATTGATATAGGCCTTCTGTTACAGATTATTAGTAATGGAAAGCTAAGCGGTGGTGAGCACAGGGTCGTTACTAACAAAAAGACTTCGCGGACGGTCAGCGGCGCCATCCATCCTTCAGGCAACTGCCATATCGAACCTGCAAAAGCACTAGTTAAGGACGGCGCAGTCCACTCTAACGATCGAGGAGGCATTACATACAAGGGTTTTGTTAGTACATTTATGACAGATCGATCATCAAGAGAGACTTCCAGCACTCGAGCGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

99

Amino Acids

10.55

Weight (kDa)

9.65

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 2 - 43 4.8e-06 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 121
AciI CCGC 3 cut(s) 81, 121, 132
AcuI CTGAAG 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 28
AhlI ACTAGT 1 cut(s) 181
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 1 cut(s) 43
AspLEI GCGC 2 cut(s) 137, 197
AsuHPI GGTGA 1 cut(s) 98
AvaI CYCGRG 1 cut(s) 288
BanI GGYRCC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 93
BccI CCATC 2 cut(s) 146, 150
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 269
BcuI ACTAGT 1 cut(s) 181
BfaI CTAG 1 cut(s) 182
BfoI RGCGCY 1 cut(s) 138
BfuAI ACCTGC 1 cut(s) 178
BisI GCNGC 1 cut(s) 133
BlpI GCTNAGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 288
BmiI GGNNCC 1 cut(s) 136
Bpu1102I GCTNAGC 1 cut(s) 77
Bsa29I ATCGAT 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 262
BseGI GGATG 2 cut(s) 138, 142
BseRI GAGGAG 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 1 cut(s) 45
BshNI GGYRCC 1 cut(s) 134
BshVI ATCGAT 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 288
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 1 cut(s) 45
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 3 cut(s) 211, 259, 263
Bsp1720I GCTNAGC 1 cut(s) 77
BspACI CCGC 3 cut(s) 81, 121, 132
BspANI GGCC 1 cut(s) 45
BspDI ATCGAT 1 cut(s) 262
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 134
BssMI GATC 3 cut(s) 211, 259, 263
BssNI GRCGYC 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 127
BstACI GRCGYC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 138, 142
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 137, 197
BstKTI GATC 3 cut(s) 214, 262, 266
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 3 cut(s) 211, 259, 263
BstMCI CGRYCG 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 32
BstUI CGCG 1 cut(s) 121
Bsu15I ATCGAT 1 cut(s) 262
BsuRI GGCC 1 cut(s) 45
BsuTUI ATCGAT 1 cut(s) 262
BtsCI GGATG 2 cut(s) 138, 142
BveI ACCTGC 1 cut(s) 178
CfoI GCGC 2 cut(s) 137, 197
ClaI ATCGAT 1 cut(s) 262
Csp6I GTAC 1 cut(s) 246
CviAII CATG 1 cut(s) 29
CviJI RGCY 3 cut(s) 13, 45, 76
CviKI_1 RGCY 3 cut(s) 13, 45, 76
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 1 cut(s) 77
DinI GGCGCC 1 cut(s) 136
DpnI GATC 3 cut(s) 213, 261, 265
DpnII GATC 3 cut(s) 211, 259, 263
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
Eco147I AGGCCT 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 288
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
FaeI CATG 1 cut(s) 32
FaiI YATR 6 cut(s) 30, 41, 162, 229, 254, 298
FalI AAGNNNNNCTT 2 cut(s) 262, 294
FatI CATG 1 cut(s) 28
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 2 cut(s) 125, 129
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 1 cut(s) 182
GlaI GCGC 2 cut(s) 136, 196
GluI GCNGC 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 1 cut(s) 45
HhaI GCGC 2 cut(s) 137, 197
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 2 cut(s) 135, 195
HinP1I GCGC 2 cut(s) 135, 195
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 270
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 56, 156
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 26, 173
HpyF3I CTNAG 1 cut(s) 77
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 2 cut(s) 135, 195
KasI GGCGCC 1 cut(s) 134
Kzo9I GATC 3 cut(s) 211, 259, 263
LpnPI CCDG 4 cut(s) 79, 135, 183, 295
MaeI CTAG 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 51, 100
MalI GATC 3 cut(s) 213, 261, 265
MboI GATC 3 cut(s) 211, 259, 263
MhlI GDGCHC 1 cut(s) 93
Mly113I GGCGCC 1 cut(s) 135
MnlI CCTC 2 cut(s) 209, 212
MseI TTAA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 132
MvnI CGCG 1 cut(s) 121
NarI GGCGCC 1 cut(s) 135
NdeII GATC 3 cut(s) 211, 259, 263
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 136
NspI RCATGY 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 288
PceI AGGCCT 1 cut(s) 45
PciI ACATGT 1 cut(s) 28
PkrI GCNGC 1 cut(s) 134
Ple19I CGATCG 1 cut(s) 214
PluTI GGCGCC 1 cut(s) 138
PscI ACATGT 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 288
PvuI CGATCG 1 cut(s) 214
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 3 cut(s) 211, 259, 263
SduI GDGCHC 1 cut(s) 93
SetI ASST 2 cut(s) 78, 172
SfoI GGCGCC 1 cut(s) 136
Sfr274I CTCGAG 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
SpeI ACTAGT 1 cut(s) 181
SseBI AGGCCT 1 cut(s) 45
SsiI CCGC 3 cut(s) 81, 121, 132
SspDI GGCGCC 1 cut(s) 134
SspMI CTAG 1 cut(s) 182
StuI AGGCCT 1 cut(s) 45
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 4 cut(s) 165, 214, 262, 289
TatI WGTACW 1 cut(s) 245
TauI GCSGC 1 cut(s) 135
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 32
XhoI CTCGAG 1 cut(s) 288
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.