Rh4AG145500

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000429
Physical Location & Seq
Reverse (-)
36824504 .. 36832678
8175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG145500.1

Sequence Viewer

Length: 717 bp
ATGGAGAAGCTTGTTTCTAGCTGGTACAATGATGTTCAACCCTTGCCCGAGACTTACATATTCCCACCCGATGCAAGACCGGGGAAGCTTAGCACTCTTCCTTCATCTGACAACATTCCAGTGATTAATCTTGGTGGTGGTGGAGCAGAAGGTCATGATCGAACTCGTATAGTTGAGCAAATACTTGGGGCTAGCCAAGAGTTCGGGTTTTTTCAGGTAATCAACCATGGCATATCAGAAAATTTGTTGCATGACACCATGGATGTGTTCAGGGAGTTTTTTGCTTTGCCTCCAGAAGACAAGGCAAGCTTCTTCTCTGAGGACCCCAACAAACTCTGCAGGCTCATAACTAGCACCGGAAATTATGACAGGGAGAATGTTCATCTTTGGCGCGATGCCCTCCGACACCCTTGTCACCCTTTAGAAAAATTCATTCCACTTTGGCCTCAACAACCGCTTAAATATCGAGAGCATGTTGGCAAATGTTCCATCCAAGTGAGGAAAGTAGCTTTGAACATTTTAGACTTGATCAGTGAAGGACTTGGGATTGGATATGGGCACTTCAGCGATGAACTTAGCCAAGAGACTCTACTCTCAGCTAATTATTATCCACCTTGTCCAGATCCAAGTTTGACATTAGGAATAACTAAACACTGTGATCCAAACCTCATCACAATTTCACTTCAAGGGGATGTACATGGCCTTCAGGTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

238

Amino Acids

26.75

Weight (kDa)

5.29

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 39 - 150 1.1e-21 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 197 - 238 4.3e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000429)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11330 FvH4_2g11330 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21630 FvH4_2g21772 FvH4_2g21772 FvH4_2g21773 FvH4_2g21774
malus_domestica MD05G1199100.v1.1 MD05G1200000.v1.1 MD05G1201900.v1.1 MD10G1188800.v1.1 MD10G1189600.v1.1 MD10G1189900.v1.1 MD15G1001500.v1.1
prunus_persica Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.1G354100_v2.0.a1 Prupe.8G224200_v2.0.a1 Prupe.8G225800_v2.0.a1
pyrus_communis pycom05g18530 pycom05g18630 pycom05g18650 pycom05g18870 pycom15g00070
rosa_chinensis RchiOBHm_Chr4g0407601 RchiOBHm_Chr6g0288311 RchiOBHm_Chr6g0288451 RchiOBHm_Chr6g0288461 RchiOBHm_Chr6g0288471 RchiOBHm_Chr6g0288491 RchiOBHm_Chr6g0288511 RchiOBHm_Chr6g0288521 RchiOBHm_Chr7g0188451
rosa_laevigata RLG00000004695 RLG00000008705 RLG00000010120 RLG00000012396 RLG00000012402 RLG00000012416 RLG00000017369
rosa_multiflora Rmu_co8451481.1_g000001 Rmu_co8483209.1_g000001 Rmu_sc0000952.1_g000001 Rmu_sc0001038.1_g000007 Rmu_sc0004806.1_g000002 Rmu_sc0006439.1_g000001 Rmu_sc0011657.1_g000015
rosa_roxburghii Rroxscaffold_3G00266420 Rroxscaffold_5G00334150 Rroxscaffold_5G00352210 Rroxscaffold_7G00180010 Rroxscaffold_7G00180020 Rroxscaffold_7G00180030 Rroxscaffold_7G00180040 Rroxscaffold_7G00180180
rosa_rugosa Rorug04G0080800 Rorug05G0399800 Rorug06G0195400 Rorug06G0195400 Rorug06G0195600 Rorug06G0196500 Rorug06G0196600 Rorug06G0490400
rosa_samantha Rh2BG181900 Rh2DG180700 Rh4AG145500 Rh4BG143300 Rh4CG152200 Rh6AG306700 Rh6AG308200 Rh6AG308300 Rh6AG308500 Rh6AG308600 Rh6BG312500 Rh6BG313400 Rh6BG313800 Rh6BG313900 Rh6CG321300 Rh6DG305500 Rh6DG306700 Rh6DG306800 Rh6DG306900 Rh6DG307100 Rh7AG095200 Rh7BG096700 Rh7CG097500
rosa_wichuraiana Rw4G011820 Rw6G026520 Rw6G026640 Rw6G026650 Rw7G008210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 411
AccII CGCG 1 cut(s) 393
AciI CCGC 1 cut(s) 455
AclWI GGATC 2 cut(s) 617, 653
AcsI RAATTY 2 cut(s) 241, 428
AcuI CTGAAG 2 cut(s) 547, 689
AfaI GTAC 2 cut(s) 26, 696
AgsI TTSAA 3 cut(s) 38, 514, 686
AluBI AGCT 6 cut(s) 10, 21, 88, 309, 509, 599
AluI AGCT 6 cut(s) 10, 21, 88, 309, 509, 599
Alw26I GTCTC 2 cut(s) 44, 578
AlwI GGATC 2 cut(s) 617, 653
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 2 cut(s) 443, 700
ApoI RAATTY 2 cut(s) 241, 428
AseI ATTAAT 1 cut(s) 126
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 407
AsuNHI GCTAGC 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 561
BarI GAAGNNNNNNTAC 1 cut(s) 687
BbsI GAAGAC 1 cut(s) 303
BccI CCATC 1 cut(s) 497
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 44, 578
BfaI CTAG 3 cut(s) 18, 192, 351
BfmI CTRYAG 1 cut(s) 337
BlpI GCTNAGC 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 61, 385
BmtI GCTAGC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 276
Bpu1102I GCTNAGC 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 80, 226, 258
BsaWI WCCGGW 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 119
BseDI CCNNGG 3 cut(s) 80, 226, 258
BseGI GGATG 3 cut(s) 268, 489, 697
BseMII CTCAG 2 cut(s) 309, 609
BseNI ACTGG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 561
Bsh1236I CGCG 1 cut(s) 393
BshFI GGCC 2 cut(s) 445, 702
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 80, 357
BsmAI GTCTC 2 cut(s) 44, 578
BsnI GGCC 2 cut(s) 445, 702
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 694
Bsp143I GATC 4 cut(s) 157, 528, 622, 658
Bsp1720I GCTNAGC 1 cut(s) 89
Bsp19I CCATGG 2 cut(s) 226, 258
BspACI CCGC 1 cut(s) 455
BspANI GGCC 2 cut(s) 445, 702
BspCNI CTCAG 2 cut(s) 310, 608
BspFNI CGCG 1 cut(s) 393
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 341
BspOI GCTAGC 1 cut(s) 195
BspPI GGATC 2 cut(s) 617, 653
BsrGI TGTACA 1 cut(s) 694
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 80, 226, 258
BssMI GATC 4 cut(s) 157, 528, 622, 658
BssT1I CCWWGG 2 cut(s) 226, 258
Bst4CI ACNGT 1 cut(s) 656
Bst6I CTCTTC 1 cut(s) 102
BstAUI TGTACA 1 cut(s) 694
BstC8I GCNNGC 3 cut(s) 193, 307, 341
BstDEI CTNAG 4 cut(s) 89, 318, 575, 595
BstDSI CCRYGG 2 cut(s) 226, 258
BstF5I GGATG 3 cut(s) 268, 489, 697
BstFNI CGCG 1 cut(s) 393
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 4 cut(s) 160, 531, 625, 661
BstMAI GTCTC 2 cut(s) 44, 578
BstMBI GATC 4 cut(s) 157, 528, 622, 658
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 1 cut(s) 393
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BsuRI GGCC 2 cut(s) 445, 702
BtgI CCRYGG 2 cut(s) 226, 258
BtgZI GCGATG 2 cut(s) 408, 582
BtsCI GGATG 3 cut(s) 268, 489, 697
BtsIMutI CAGTG 3 cut(s) 126, 538, 652
Cac8I GCNNGC 3 cut(s) 193, 307, 341
CciI TCATGA 1 cut(s) 154
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 25, 695
CviAII CATG 6 cut(s) 155, 227, 251, 259, 473, 698
CviQI GTAC 2 cut(s) 25, 695
DdeI CTNAG 4 cut(s) 89, 318, 575, 595
DpnI GATC 4 cut(s) 159, 530, 624, 660
DpnII GATC 4 cut(s) 157, 528, 622, 658
DrdI GACNNNNNNGTC 1 cut(s) 411
DseDI GACNNNNNNGTC 1 cut(s) 411
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 226, 258
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 547, 689
Eco88I CYCGRG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 226, 258
ErhI CCWWGG 2 cut(s) 226, 258
FaeI CATG 6 cut(s) 158, 230, 254, 262, 476, 701
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FatI CATG 6 cut(s) 154, 226, 250, 258, 472, 697
FbaI TGATCA 1 cut(s) 528
FokI GGATG 3 cut(s) 275, 476, 704
FspBI CTAG 3 cut(s) 18, 192, 351
GlaI GCGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 2 cut(s) 445, 702
HapII CCGG 2 cut(s) 80, 357
HhaI GCGC 1 cut(s) 393
Hin1II CATG 6 cut(s) 158, 230, 254, 262, 476, 701
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HindIII AAGCTT 3 cut(s) 8, 86, 307
HinfI GANTC 1 cut(s) 586
HpaII CCGG 2 cut(s) 80, 357
HphI GGTGA 1 cut(s) 407
Hpy188I TCNGA 4 cut(s) 109, 238, 319, 404
Hpy188III TCNNGA 4 cut(s) 155, 293, 467, 620
HpyAV CCTTC 4 cut(s) 111, 143, 530, 713
HpyCH4III ACNGT 1 cut(s) 656
HpyCH4V TGCA 3 cut(s) 74, 250, 339
HpyF3I CTNAG 4 cut(s) 89, 318, 575, 595
Hsp92II CATG 6 cut(s) 158, 230, 254, 262, 476, 701
HspAI GCGC 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 4 cut(s) 157, 528, 622, 658
LmnI GCTCC 1 cut(s) 143
LweI GCATC 2 cut(s) 61, 385
MaeI CTAG 3 cut(s) 18, 192, 351
MaeIII GTNAC 1 cut(s) 413
MalI GATC 4 cut(s) 159, 530, 624, 660
MboI GATC 4 cut(s) 157, 528, 622, 658
MboII GAAGA 3 cut(s) 89, 304, 308
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 561
MluCI AATT 5 cut(s) 241, 361, 428, 601, 675
MlyI GAGTC 1 cut(s) 580
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 6 cut(s) 300, 313, 410, 456, 492, 677
MseI TTAA 2 cut(s) 126, 459
MslI CAYNNNNRTG 3 cut(s) 119, 263, 494
MspI CCGG 2 cut(s) 80, 357
MspR9I CCNGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 393
NciI CCSGG 1 cut(s) 81
NcoI CCATGG 2 cut(s) 226, 258
NdeII GATC 4 cut(s) 157, 528, 622, 658
NheI GCTAGC 1 cut(s) 191
NlaIII CATG 6 cut(s) 158, 230, 254, 262, 476, 701
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 1 cut(s) 476
PagI TCATGA 1 cut(s) 154
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PpuMI RGGWCCY 1 cut(s) 322
PshBI ATTAAT 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 322
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PstI CTGCAG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 622
RsaI GTAC 2 cut(s) 26, 696
RsaNI GTAC 2 cut(s) 25, 695
RseI CAYNNNNRTG 3 cut(s) 119, 263, 494
SaqAI TTAA 2 cut(s) 126, 459
Sau3AI GATC 4 cut(s) 157, 528, 622, 658
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 1 cut(s) 580
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 2 cut(s) 61, 385
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 3 cut(s) 119, 263, 494
Sse9I AATT 5 cut(s) 241, 361, 428, 601, 675
SsiI CCGC 1 cut(s) 455
SspMI CTAG 3 cut(s) 18, 192, 351
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 2 cut(s) 226, 258
TaaI ACNGT 1 cut(s) 656
TaqI TCGA 2 cut(s) 160, 466
TasI AATT 5 cut(s) 241, 361, 428, 601, 675
TatI WGTACW 1 cut(s) 694
Tru1I TTAA 2 cut(s) 126, 459
Tru9I TTAA 2 cut(s) 126, 459
TscAI CASTG 3 cut(s) 126, 538, 659
TseFI GTSAC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 4 cut(s) 93, 371, 421, 585
TspRI CASTG 3 cut(s) 126, 538, 659
VpaK11BI GGWCC 1 cut(s) 322
VspI ATTAAT 1 cut(s) 126
XapI RAATTY 2 cut(s) 241, 428
XceI RCATGY 1 cut(s) 476
XspI CTAG 3 cut(s) 18, 192, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.