RNA splicing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 48, 299 |
| AcsI | RAATTY | 1 cut(s) | 402 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 296 |
| AgsI | TTSAA | 4 cut(s) | 16, 131, 261, 317 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 220 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 389, 421 |
| AluBI | AGCT | 2 cut(s) | 119, 138 |
| AluI | AGCT | 2 cut(s) | 119, 138 |
| Alw21I | GWGCWC | 1 cut(s) | 84 |
| Alw26I | GTCTC | 2 cut(s) | 162, 263 |
| AlwI | GGATC | 2 cut(s) | 48, 299 |
| AoxI | GGCC | 3 cut(s) | 73, 164, 195 |
| ApoI | RAATTY | 1 cut(s) | 402 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 390 |
| AspS9I | GGNCC | 4 cut(s) | 73, 196, 247, 278 |
| AsuHPI | GGTGA | 1 cut(s) | 76 |
| AvaII | GGWCC | 2 cut(s) | 247, 278 |
| AxyI | CCTNAGG | 1 cut(s) | 168 |
| BbsI | GAAGAC | 2 cut(s) | 216, 219 |
| Bbv12I | GWGCWC | 1 cut(s) | 84 |
| BccI | CCATC | 1 cut(s) | 346 |
| BcoDI | GTCTC | 2 cut(s) | 162, 263 |
| BfaI | CTAG | 1 cut(s) | 68 |
| BglII | AGATCT | 1 cut(s) | 411 |
| Bme18I | GGWCC | 2 cut(s) | 247, 278 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 220 |
| BmgT120I | GGNCC | 4 cut(s) | 73, 196, 247, 278 |
| BmiI | GGNNCC | 3 cut(s) | 249, 280, 393 |
| BpiI | GAAGAC | 2 cut(s) | 216, 219 |
| BpuEI | CTTGAG | 1 cut(s) | 93 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 296 |
| Bse21I | CCTNAGG | 1 cut(s) | 168 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 296 |
| BseMII | CTCAG | 1 cut(s) | 182 |
| BshFI | GGCC | 3 cut(s) | 75, 166, 197 |
| BsiHKAI | GWGCWC | 1 cut(s) | 84 |
| BslFI | GGGAC | 1 cut(s) | 172 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 296 |
| BsmAI | GTCTC | 2 cut(s) | 162, 263 |
| BsmFI | GGGAC | 1 cut(s) | 172 |
| BsnI | GGCC | 3 cut(s) | 75, 166, 197 |
| Bsp1286I | GDGCHC | 1 cut(s) | 84 |
| Bsp143I | GATC | 3 cut(s) | 40, 304, 411 |
| BspANI | GGCC | 3 cut(s) | 75, 166, 197 |
| BspCNI | CTCAG | 1 cut(s) | 181 |
| BspLI | GGNNCC | 3 cut(s) | 249, 280, 393 |
| BspPI | GGATC | 2 cut(s) | 48, 299 |
| BssMI | GATC | 3 cut(s) | 40, 304, 411 |
| Bst6I | CTCTTC | 2 cut(s) | 201, 381 |
| BstC8I | GCNNGC | 1 cut(s) | 121 |
| BstDEI | CTNAG | 2 cut(s) | 168, 378 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 294 |
| BstKTI | GATC | 3 cut(s) | 43, 307, 414 |
| BstMAI | GTCTC | 2 cut(s) | 162, 263 |
| BstMBI | GATC | 3 cut(s) | 40, 304, 411 |
| BstV2I | GAAGAC | 2 cut(s) | 216, 219 |
| BstX2I | RGATCY | 1 cut(s) | 411 |
| BstYI | RGATCY | 1 cut(s) | 411 |
| Bsu36I | CCTNAGG | 1 cut(s) | 168 |
| BsuRI | GGCC | 3 cut(s) | 75, 166, 197 |
| Cac8I | GCNNGC | 1 cut(s) | 121 |
| Cfr13I | GGNCC | 4 cut(s) | 73, 196, 247, 278 |
| CviAII | CATG | 1 cut(s) | 92 |
| CviJI | RGCY | 7 cut(s) | 58, 75, 107, 119, 138, 166, 197 |
| CviKI_1 | RGCY | 7 cut(s) | 58, 75, 107, 119, 138, 166, 197 |
| DdeI | CTNAG | 2 cut(s) | 168, 378 |
| DpnI | GATC | 3 cut(s) | 42, 306, 413 |
| DpnII | GATC | 3 cut(s) | 40, 304, 411 |
| DriI | GACNNNNNGTC | 1 cut(s) | 220 |
| Eam1104I | CTCTTC | 2 cut(s) | 201, 381 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 220 |
| EarI | CTCTTC | 2 cut(s) | 201, 381 |
| Eco147I | AGGCCT | 1 cut(s) | 166 |
| Eco47I | GGWCC | 2 cut(s) | 247, 278 |
| Eco81I | CCTNAGG | 1 cut(s) | 168 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 294 |
| EcoO109I | RGGNCCY | 2 cut(s) | 247, 278 |
| FaeI | CATG | 1 cut(s) | 95 |
| FaiI | YATR | 3 cut(s) | 93, 126, 141 |
| FaqI | GGGAC | 1 cut(s) | 172 |
| FatI | CATG | 1 cut(s) | 91 |
| FspBI | CTAG | 1 cut(s) | 68 |
| HaeIII | GGCC | 3 cut(s) | 75, 166, 197 |
| Hin1II | CATG | 1 cut(s) | 95 |
| HindIII | AAGCTT | 1 cut(s) | 117 |
| HinfI | GANTC | 1 cut(s) | 221 |
| HphI | GGTGA | 1 cut(s) | 76 |
| Hpy166II | GTNNAC | 1 cut(s) | 433 |
| Hpy188I | TCNGA | 2 cut(s) | 45, 396 |
| Hpy188III | TCNNGA | 2 cut(s) | 110, 170 |
| Hpy8I | GTNNAC | 1 cut(s) | 433 |
| HpyAV | CCTTC | 5 cut(s) | 40, 238, 376, 391, 415 |
| HpyCH4IV | ACGT | 1 cut(s) | 216 |
| HpyCH4V | TGCA | 2 cut(s) | 371, 427 |
| HpyF3I | CTNAG | 2 cut(s) | 168, 378 |
| HpySE526I | ACGT | 1 cut(s) | 216 |
| Hsp92II | CATG | 1 cut(s) | 95 |
| Kzo9I | GATC | 3 cut(s) | 40, 304, 411 |
| LpnPI | CCDG | 4 cut(s) | 134, 155, 264, 297 |
| MaeI | CTAG | 1 cut(s) | 68 |
| MaeII | ACGT | 1 cut(s) | 216 |
| MalI | GATC | 3 cut(s) | 42, 306, 413 |
| MboI | GATC | 3 cut(s) | 40, 304, 411 |
| MboII | GAAGA | 8 cut(s) | 187, 215, 216, 218, 224, 333, 336, 398 |
| MflI | RGATCY | 1 cut(s) | 411 |
| MhlI | GDGCHC | 1 cut(s) | 84 |
| MluCI | AATT | 2 cut(s) | 237, 402 |
| MlyI | GAGTC | 1 cut(s) | 230 |
| MnlI | CCTC | 5 cut(s) | 73, 177, 202, 300, 382 |
| MroXI | GAANNNNTTC | 1 cut(s) | 390 |
| MseI | TTAA | 2 cut(s) | 9, 417 |
| NdeII | GATC | 3 cut(s) | 40, 304, 411 |
| NlaIII | CATG | 1 cut(s) | 95 |
| NlaIV | GGNNCC | 3 cut(s) | 249, 280, 393 |
| PceI | AGGCCT | 1 cut(s) | 166 |
| PdmI | GAANNNNTTC | 1 cut(s) | 390 |
| PleI | GAGTC | 1 cut(s) | 229 |
| PpsI | GAGTC | 1 cut(s) | 229 |
| PpuMI | RGGWCCY | 2 cut(s) | 247, 278 |
| Psp5II | RGGWCCY | 2 cut(s) | 247, 278 |
| PspN4I | GGNNCC | 3 cut(s) | 249, 280, 393 |
| PspPI | GGNCC | 4 cut(s) | 73, 196, 247, 278 |
| PspPPI | RGGWCCY | 2 cut(s) | 247, 278 |
| PsuI | RGATCY | 1 cut(s) | 411 |
| SaqAI | TTAA | 2 cut(s) | 9, 417 |
| Sau3AI | GATC | 3 cut(s) | 40, 304, 411 |
| Sau96I | GGNCC | 4 cut(s) | 73, 196, 247, 278 |
| SchI | GAGTC | 1 cut(s) | 230 |
| SduI | GDGCHC | 1 cut(s) | 84 |
| SetI | ASST | 9 cut(s) | 51, 65, 121, 140, 219, 292, 301, 393, 426 |
| SinI | GGWCC | 2 cut(s) | 247, 278 |
| SmlI | CTYRAG | 1 cut(s) | 108 |
| SmoI | CTYRAG | 1 cut(s) | 108 |
| Sse9I | AATT | 2 cut(s) | 237, 402 |
| SseBI | AGGCCT | 1 cut(s) | 166 |
| SspMI | CTAG | 1 cut(s) | 68 |
| StuI | AGGCCT | 1 cut(s) | 166 |
| TaiI | ACGT | 1 cut(s) | 219 |
| TasI | AATT | 2 cut(s) | 237, 402 |
| Tru1I | TTAA | 2 cut(s) | 9, 417 |
| Tru9I | TTAA | 2 cut(s) | 9, 417 |
| TspDTI | ATGAA | 2 cut(s) | 80, 342 |
| VpaK11BI | GGWCC | 2 cut(s) | 247, 278 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 294 |
| XapI | RAATTY | 1 cut(s) | 402 |
| XmnI | GAANNNNTTC | 1 cut(s) | 390 |
| XspI | CTAG | 1 cut(s) | 68 |