pycom13g23210

protein kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000437
Physical Location & Seq
Forward (+)
20869049 .. 20876287
7239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g23210.3

Sequence Viewer

Length: 5625 bp
ATGACCTTTTTTACTAGCTTGAGGGTGCCTCTGAAGATCGAGCATGAGGATGTCCAGAATACAGCATTTCGAACTCATTATGGTCATTACAAGTTTCTAGTGATGCCATTCAGGTTAACACATGCTCCATCAACCCTTATGGACCTGATGAATCAGGTATTTCTCTTGTATCACGTCAGGTTCGTTATTGTCTTCATTGATGATATTTTGGTATATTCTAAATCTAAGGCGGAGCATACTAGACATCTCACTCTTGTTCTAAAGAAGTTGAGAGAACATCAACTTGTCATTCTTGGGACATGTGATATCAGCCCAAGTTTTCTTGTAGACCCTCAGAAGGTAGATGCAGTAAAGAATTGGGAACAACCTTGTACTGTCACGGAAGTACGGAGTTTTCTTGGTCTAGCTGGCTATTATCGACATTTTGTGAAAGATTTCTTAGCTATAGCTCTACCATTAATGAAGTTAACTAGGAAGGGAGTTAATTTCGAGTGGAGTGATGATTGTGAGCAGAGTTTTCAGCAACTGAAGTATTGCCTCACTCATGCACTTGTGTTAGCAGTTCCATATGACAATGGGAATTTCGAGATTTATAGTGATGCTTCTTTAAATGGTTTGGGCTGTGTACTAATGCAGCATGGTAGGTATCCCACTCATGATCTCGGGTTAGCATCCGTTATCTTTGCATTGAAAATTTGGAGGCATTATTTGTATGGTTTTACTCAGAAAGATCTTAATCTCAGGCAACAGAGATGGGTTGAGTTGTTTAATGATTATGACTACACGATTGAGTATTATACTTGTCGTGCAAACTCTGTAGTTGATGCTTTAAGCAGGAAATCCCAAGGTCGACTTAACACACTTTATGGATGCACTTTACTTGCTAATTTTCAAGTTAGGCTAATTTTGCTAGATCGTGTACTCGAAGCTCAGGCGAATGATTCGAAATCTCAGGAGATTAGGAATTTTGATGGTATGCTGATGCAGGGTGACCGTGCGTGTTTCAGCATATGTGATGCATCCTGGGAGTACCAAGATGTATCATACCATCCGACCTTTCTATTACTAGCCTGGTATGAAAAGAGAAATCACAGAATATGTAAGTCGTTGTGCAGTTTGTTAGCAAGTCAAGGCAGAAACGAAGAAACCTACTTCCCGTACCTCAATGGAAATGGGAAGATATTACCATGGATTTCGTGTACAAGTTACCTCGTATGCGGAATGTCAGCACACTTAATGCCCGTTCGAGAAAATTACTCGTTGAGTCAATTGGCAGAATGGCTCATTTCAAAAATTGTCAAATATCATGGTGTTCCGGTGAGTATCATTTATGATCAGGATCCCAGGTTCACTTCTAAATTTTTGGTAGCTTTCCAAGAAGCTCTCGATTCAAGATTTCTTTACAGCACAGCTTATCACCCTCAGATAGATGGACAATCTGAAAGAACCATTCAGATGCTAGAAGATATGTTGAGATCCTCAATTTTATATTATCATTCTAGCATTGGCATGTTACCTTTCGGGGCGTTTTATGGTAAACCATATCGTACCCCGTTATGTTGGTCTGAAGTTGGCGAGAGAGTGTTAGTGGACCCTAAGATTGTGGATGAGACCACGCAGAATATTCGGGTGATTAAAGCTAACCTGAAGGTGGCATTGGGTATTTTGAAGTTGTCACCATGGAAAGGCGTGGTACGGTTCAGAAAGAATGGTAAGTTAATTCCCCATTATATTGGACCTTATCAGATCACCGAACGAGTTGGTGAAGTTGCCTATCGGCTTGAGCTACCTCTAGAATTATCAAAGGTACATAACGTGTTTCACGTGTCTATGCTACGTAAATATATCCCTGATCCGTCGCATGTAATTCCTCCTCAGCCACTGGAGATTAACCCAGACTTGACTTACAATGAGGAGCTGGTAATGATTTTGGATTGGAAAGACAAGGTTCTTCGGAACAAGACCGTTCACATGTTGAAAATTTTGTGGAGGAACCACTCAGTCGAGGAAGTTACCTGGGAAACGGAATATTGTATGCGGGACTTATACCCATTTTCTCCTCAAGCGTGTAAAACGATGAATATGCCCTTTATTTTTCTCACTATCGTAATTAAATCATACTCGACGTCACGAGCGTGTTGTCGTGAGTTAGGGCCAAATCGGAGTAATAACGGAAAAGGTTACGAACAACTGAATGCCCAAACTCAACACTTATCTACCCAATTATTTAGGCCCTTATATCCTATGGCCCTGTCAGAAAATATTTCTTTTCTTATTCAGACATGCGCACACACATGTACACTCTCATTCTATCTCCTTTTCGATTCCGCTTCTTTCTTCTTCCTTTTCAAACTTGAGCTTTACATCATCATATTCCCCTTGACCTCACGAACTCAAAACCAGAAGCTCCGACAAGGAGCAAGTTTACTCCGTCATGATTTTGGCAAGTTGACGGAGTCTAAGGACCTAGAAAAGTCCCCACGCAACTTCCCTAGAACTTTGGAGCAAGTTTGGATTTTGGTCGAAATTGTCAAAGGATTTTCCGACCGTTTTCTATTGGATTTTGGACTTCTAATAGGTACAAACGTGTTTTACTCTTCGAGACCTTCAAATCCATATAAATATCTTGCATTTTGGTTAAGAAATGAATGCAGCAGCGGCGGACAGCAGAAACCGACGGGTGACCGGAGAAGAAGAAGGTATATTCTGTTAACTTTAACAAAATATTCTAATGGCTTTGACGGAAAATTCCTAACACCATTAGGGTTTCCGTTAGTGTGCCATGTACGTGCCTACACGTGGCCGTGCCTTGGCCGGCGCTTGAGGGCTCCTCCGGTGTCCAAAAATCTTTCTAAAAATTTGTGTGGATTCGTATCATTGAGTAGATCATGTTTGTATATTCAAACCTTACATTTGAGCAAACTACGAGGAGTTATTCCTAGATTTCATATATGTGAGATGTACCCCGAGGACGAGCGTGGACAAGCGAAGCTAGAGGGCTACGATCCTGGGCATTTTTTTCTATATATATATATATATATATACACACACACATATATGGTTTCTATAAACATTTTTATATGGAGTGATAGGAGTGTATTTATGCGACTTTGTAAGCTATTTTCGTGTGTTTGTAGGTTTAAATGGCAAAAGAGGCAAGAAAGTGCATTTTGGGGCATTTTAGAGCAATTCTGGGCTGGAATGGATAGCTTGCATGTGGAGCAAGGTGGTTGGATGAATTTGAAGTTCAAGAAGGGCTTGGAATCTAAGAAGAAGGAAACATTATCCAAATTACCTTATCTTATCCAAACCTTATCCAACCATATCTTATCTTATCCTAACCTAATCTTTTGCTACCTTAATTCCAGCTCTCCTACATTGGTGCTACACCCCTTATCATTTGTCCTTCTAGAAACCTGTCACAATATCCTTTCTAGAAGCCTAGTTTCTACCGAATTACCCAAGGATTGTGCTATCTTTTTCCTTCTCAACTTGTGCCATGCCAAGGCTTTGTGCCGTGAAACTCTAGCATATAAATACATAAATCTACAAAGAGTGTGCCACAGGTTTCTGAGAGCTTTTAGAGACTTGTGCCATGCAAGGAAGCAAGGAGAGGACGAGTGCCGTGCCTGCTACCATCCAAGGGAGTACTGTTGCCTACTTTGGAGTGTTTATTTTAATTTGGTTTTCAATTATGATGAACATAAGGAACTAAATTCTTTTTATTCCTCCAGTTATTGTTTTGTAAAACATGAATGCGATTTACTTATTTGTTTGATTGATAACTTATTCTTGTATACAAGGGAATTTCACCTAATCGAAAAGGAAGAACAAGACATGGCTGTAGACAATCGAACAATCAAGGAACTTTCAGCCTCGGGGTTGGACAATGCCATGCCCATTTGCATTCAATACCCTAGGGCAGCTCCGGACAAGGCTGATGAGTTTGAATTGAAGTCTAGGTTGCTGCATCACATTCCCAAATACCATGGGCTGTTCATGGAAGATCCGAACAAGCATTTGAAGGAATTTGAAGTACTATGCTCGAGTAAGACACCCGTTAATGTGGATGGGAATATTTTGAAGATGAAAGGTTTTCCATTCTCTTTTTTGGAAAAGGCTAAGGATTGGTTATACAAATTGGCACCTGCAATCATCACTTCTTGGGAAAGCATGAAGAGAGCATTCTTAGAGAAAATTTTCCCAACTTCGAGAGGAGAATCCTTTCCAGCATATTATGAGCGGTTCAAGACTTTAGTTGCATCATGCCCTCAACATCAAATGAAAGAAGAGCTAGATGCTTCGGCAGGTGGTGCTTTGGTTGACAAAACCCCAGTTGCTGCCAAGATCATAATTGCAAATCGAGCCTTAAATGCACAACAATACGAAGGAGTTGGACAAAGAGACCCCCACGACAACACCAAGTTAATGAGCCAAAGCACAAAGAGCAAGTGTTTATGGCGTGTGCACTATGCAAGGACATTCCAATCCAATGATCAATGTCCTCAATTGATAGAAAATGAAGGATGGGAAAGTGCTAATGCCATAGGATATCAAGGCCAAAATCAACCACGACATGACCCATTCTCTAACATTTACAATTCAGGTTGGAGAGATCATCCAAATATGAAGTGGAGGGAACCTCAACAAACTCATCAACAAGGCGGATATAGACAGCCACCTCCGGGACTCTATCAAAGGCCATTCGCACCAAATGATAAAATTCTTCAAGTACTAACTTCTTTGACGCAGGGCTTACAAAACCAGGCCAAGGAGATGGGCGAATTAAAGAATCAAATAGGGCAGATGGCGGAGTTCATGGGACAGTTTAGAGAAGAACGCAAACTACCTAGTTCAACCATTGTGAATCCAAAGGGAGGTTTTGAAACCGCTAAGGCTATCGCATTGAGAAGTGGCAAGGAAGACGAAAGGTTGCTACTCGAGGAAAAAAAAGATTCCAAAGCCACGGCAAGGGTAGAACAACCCATGCCGCAGGCCCCTAATGCTCCTAAGTTGTCCAATTCACGTAAGGGTGGTTCAAATTCAATTCATTCTAACCCTATTCCACCAAATGTGACTTTCCCTCACAGGTTTATGCAATCTAATAAAGAAGATAATGAAAAAGACATCTTAGAGACTTTTAGGAAGGTACAAGTCAATATCCTACTTCTTGATGCAATAAAGCAGGTTCCAAGGTATGCTAAATTCTTGAAAGAGCTTTGTAAAACAAGGAGAAGAATTTCGAACAAAGAAGTGGTGAGGGTAAGTGAGAATGTTTCAGCAGTTTTGCAACGAAAACTACCACCTAAATGCAAAGCTCCAGGTAGTTTCACAATCCTTTGTGTTATTGGTAATACCAAGTTTGAACATGCTATGTTAGATTTAGGTGCTTCCATTAATGTCATGTCCTACTCTATTTATGCATCTATGAACCTAGGAGAGCTTAAAAATGATGGTGTTATAATTCAATTAGCCGATCGTTCTAATGCATATCCGAAAGGAGTTTTGGAAGATGTTTTGGTGCAGGTTGACCATTTGATATTTCCAGCAGACTTTTATGTGCTTGACATGGAAGATTCAGCCATTTTACACCATTGCCAATCCTACTTGGGAGACTTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 RVT_1 Retrotrans_gag rve

Protein Analysis

1875

Amino Acids

216.86

Weight (kDa)

8.88

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06105
fragaria_vesca FvH4_1g09709 FvH4_4g02101 FvH4_4g08975 FvH4_6g23372 FvH4_6g39851 FvH4_c1g00300
malus_domestica MD01G1176100.v1.1 MD09G1284900.v1.1 MD15G1306600.v1.1
prunus_persica Prupe.1G227700_v2.0.a1
pyrus_communis pycom01g00630 pycom01g01020 pycom01g01690 pycom01g01760 pycom01g04190 pycom01g04400 pycom02g18130 pycom03g10160 pycom05g06370 pycom05g08930 pycom06g05590 pycom10g06320 pycom11g14330 pycom11g15320 pycom11g16240 pycom12416g00050 pycom12420g00010 pycom12420g00120 pycom12426g00180 pycom12426g00710 pycom12531g00010 pycom1256g00020 pycom1256g00050 pycom1341g00030 pycom1353g00030 pycom13g23070 pycom13g23210 pycom13g26260 pycom13g26370 pycom13g26470 pycom13g26980 pycom14g08550 pycom14g08850 pycom1534g00040 pycom15g28760 pycom15g31230 pycom1604g00040 pycom16g21900 pycom16g26030 pycom1739g00020 pycom1739g00050 pycom17g12970 pycom17g17960 pycom17g18300 pycom17g18650 pycom2172g00020 pycom2438g00020 pycom436g00370 pycom436g00390 pycom576g00050 pycom76g00020
rosa_chinensis RchiOBHm_Chr1g0320811 RchiOBHm_Chr2g0135321 RchiOBHm_Chr3g0485721 RchiOBHm_Chr7g0236571
rosa_multiflora Rmu_sc0000185.1_g000007 Rmu_sc0002094.1_g000030 Rmu_sc0002764.1_g000007 Rmu_sc0002809.1_g000014 Rmu_sc0003005.1_g000007 Rmu_sc0003798.1_g000005 Rmu_sc0004646.1_g000027 Rmu_sc0004665.1_g000009 Rmu_sc0004818.1_g000001 Rmu_sc0004988.1_g000031 Rmu_sc0006373.1_g000007 Rmu_sc0007142.1_g000015 Rmu_sc0009930.1_g000001 Rmu_sc0011451.1_g000001 Rmu_sc0020424.1_g000001 Rmu_sc0036944.1_g000001 Rmu_ssc0000062.1_g000010
rosa_roxburghii Rroxscaffold_2G00123930 Rroxscaffold_3G00231480 Rroxscaffold_6G00413880
rosa_rugosa Rorug04G0084800
rosa_samantha Rh3BG150500 Rh6DG232400
rosa_wichuraiana Rw2G018400 Rw3G027860 Rw4G021220 Rw6G020670 Rw6G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5462
AarI CACCTGC 2 cut(s) 4156, 4298
AatII GACGTC 1 cut(s) 2129
Acc16I TGCGCA 1 cut(s) 2287
Acc36I ACCTGC 4 cut(s) 4156, 4298, 5176, 5515
AccB1I GGYRCC 2 cut(s) 25, 4144
AccBSI CCGCTC 1 cut(s) 4243
AccI GTMKAC 4 cut(s) 327, 850, 3797, 3846
AccIII TCCGGA 1 cut(s) 3928
AclWI GGATC 6 cut(s) 1334, 1347, 1470, 1847, 2999, 4001
AcoI YGGCCR 2 cut(s) 2801, 2812
AcuI CTGAAG 4 cut(s) 53, 548, 1587, 1667
AcvI CACGTG 2 cut(s) 1825, 2799
AcyI GRCGYC 1 cut(s) 2126
AflIII ACRYGT 7 cut(s) 299, 1815, 1824, 1971, 2294, 2586, 2796
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 7 cut(s) 1022, 1070, 1343, 2015, 3007, 4764, 5320
AjuI GAANNNNNNNTTGG 4 cut(s) 1640, 1672, 5489, 5521
AleI CACNNNNGTG 1 cut(s) 2134
AloI GAACNNNNNNTCC 2 cut(s) 1331, 1363
Alw21I GWGCWC 1 cut(s) 4470
Alw26I GTCTC 6 cut(s) 1604, 2596, 3579, 4398, 5129, 5607
Alw44I GTGCAC 1 cut(s) 4466
AlwI GGATC 6 cut(s) 1334, 1347, 1470, 1847, 2999, 4001
AlwNI CAGNNNCTG 3 cut(s) 526, 1882, 4340
Ama87I CYCGRG 5 cut(s) 662, 2966, 3877, 4045, 4940
Aor13HI TCCGGA 1 cut(s) 3928
AoxI GGCC 9 cut(s) 2153, 2231, 2247, 2801, 2812, 4558, 4700, 4767, 4995
ApaLI GTGCAC 1 cut(s) 4466
ApeKI GCWGC 6 cut(s) 634, 2652, 2655, 3923, 3967, 4340
AseI ATTAAT 2 cut(s) 458, 5397
Asp700I GAANNNNTTC 4 cut(s) 434, 4199, 4224, 5239
AspA2I CCTAGG 2 cut(s) 3917, 5434
AspLEI GCGC 2 cut(s) 2288, 2820
AspS9I GGNCC 8 cut(s) 142, 1591, 1736, 2153, 2232, 2248, 2464, 4996
AsuC2I CCSGG 1 cut(s) 4686
AsuII TTCGAA 3 cut(s) 70, 944, 5243
AvaI CYCGRG 5 cut(s) 662, 2966, 3877, 4045, 4940
AvaII GGWCC 4 cut(s) 142, 1591, 1736, 2464
AvrII CCTAGG 2 cut(s) 3917, 5434
BaeGI GKGCMC 1 cut(s) 4470
BaeI ACNNNNGTAYC 2 cut(s) 2571, 2604
BamHI GGATCC 1 cut(s) 1339
BanI GGYRCC 2 cut(s) 25, 4144
BanII GRGCYC 1 cut(s) 2830
BauI CACGAG 1 cut(s) 2130
BbrPI CACGTG 2 cut(s) 1825, 2799
BbsI GAAGAC 2 cut(s) 184, 4929
Bbv12I GWGCWC 1 cut(s) 4470
BbvCI CCTCAGC 1 cut(s) 1875
BbvI GCAGC 6 cut(s) 646, 2664, 2667, 3935, 3954, 4327
BceAI ACGGC 4 cut(s) 2788, 3501, 3609, 4983
BcgI CGANNNNNNTGC 6 cut(s) 1607, 1641, 2065, 2099, 3608, 3642
BciT130I CCWGG 7 cut(s) 1024, 1072, 1345, 2017, 3009, 4766, 5322
BciVI GTATCC 1 cut(s) 657
BclI TGATCA 2 cut(s) 1333, 4495
BcnI CCSGG 1 cut(s) 4686
BcoDI GTCTC 6 cut(s) 1604, 2596, 3579, 4398, 5129, 5607
BfmI CTRYAG 3 cut(s) 444, 816, 3843
BfoI RGCGCY 1 cut(s) 2821
BfuAI ACCTGC 4 cut(s) 4156, 4298, 5176, 5515
BfuI GTATCC 1 cut(s) 657
BglII AGATCT 1 cut(s) 730
BisI GCNGC 8 cut(s) 635, 2653, 2656, 2659, 3924, 3968, 4341, 4991
BlnI CCTAGG 2 cut(s) 3917, 5434
BlsI GCNGC 8 cut(s) 636, 2654, 2657, 2660, 3925, 3969, 4342, 4992
BmcAI AGTACT 3 cut(s) 3650, 4038, 4734
Bme1390I CCNGG 8 cut(s) 1024, 1072, 1345, 2017, 3009, 4686, 4766, 5322
Bme18I GGWCC 4 cut(s) 142, 1591, 1736, 2464
BmeT110I CYCGRG 5 cut(s) 662, 2966, 3877, 4045, 4940
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 8 cut(s) 142, 1591, 1736, 2153, 2232, 2248, 2464, 4996
BmiI GGNNCC 9 cut(s) 27, 1341, 1593, 1994, 2829, 4146, 4641, 4998, 5190
BmrFI CCNGG 8 cut(s) 1024, 1072, 1345, 2017, 3009, 4686, 4766, 5322
BmrI ACTGGG 1 cut(s) 4328
BmuI ACTGGG 1 cut(s) 4328
BpiI GAAGAC 2 cut(s) 184, 4929
BplI GAGNNNNNCTC 6 cut(s) 13, 45, 2815, 2847, 4627, 4659
BpmI CTGGAG 3 cut(s) 1904, 3715, 5304
Bpu10I CCTNAGC 4 cut(s) 930, 1875, 4122, 4893
Bpu14I TTCGAA 3 cut(s) 70, 944, 5243
BpuEI CTTGAG 5 cut(s) 40, 1802, 2046, 2375, 2842
BpuMI CCSGG 1 cut(s) 4686
BsaAI YACGTR 5 cut(s) 1825, 1838, 2789, 2799, 5027
BsaBI GATNNNNATC 3 cut(s) 735, 1338, 2872
BsaHI GRCGYC 1 cut(s) 2126
BsaI GGTCTC 3 cut(s) 1604, 2596, 4398
BsaWI WCCGGW 4 cut(s) 1315, 2685, 2833, 3928
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bse118I RCCGGY 1 cut(s) 2814
Bse1I ACTGG 3 cut(s) 1887, 3732, 4334
Bse3DI GCAATG 1 cut(s) 5593
Bse8I GATNNNNATC 3 cut(s) 735, 1338, 2872
BseAI TCCGGA 1 cut(s) 3928
BseBI CCWGG 7 cut(s) 1024, 1072, 1345, 2017, 3009, 4766, 5322
BseJI GATNNNNATC 3 cut(s) 735, 1338, 2872
BseMI GCAATG 1 cut(s) 5593
BseMII CTCAG 9 cut(s) 347, 737, 754, 944, 965, 1436, 1889, 2013, 3563
BseNI ACTGG 3 cut(s) 1887, 3732, 4334
BseRI GAGGAG 6 cut(s) 1863, 1928, 2049, 2820, 2943, 4230
BseSI GKGCMC 1 cut(s) 4470
BseXI GCAGC 6 cut(s) 646, 2664, 2667, 3935, 3954, 4327
BsgI GTGCAG 2 cut(s) 1132, 5543
Bsh1285I CGRYCG 2 cut(s) 2548, 5479
BshFI GGCC 9 cut(s) 2155, 2233, 2249, 2803, 2814, 4560, 4702, 4769, 4997
BshNI GGYRCC 2 cut(s) 25, 4144
BsiEI CGRYCG 2 cut(s) 2548, 5479
BsiHKAI GWGCWC 1 cut(s) 4470
BsiHKCI CYCGRG 5 cut(s) 662, 2966, 3877, 4045, 4940
BsiSI CCGG 6 cut(s) 1316, 2686, 2815, 2834, 3929, 4685
BslFI GGGAC 5 cut(s) 310, 2054, 2461, 4701, 4836
BsmAI GTCTC 6 cut(s) 1604, 2596, 3579, 4398, 5129, 5607
BsmFI GGGAC 5 cut(s) 310, 2054, 2461, 4701, 4836
BsmI GAATGC 5 cut(s) 2200, 2654, 3761, 3906, 4184
BsnI GGCC 9 cut(s) 2155, 2233, 2249, 2803, 2814, 4560, 4702, 4769, 4997
Bso31I GGTCTC 3 cut(s) 1604, 2596, 4398
BsoBI CYCGRG 5 cut(s) 662, 2966, 3877, 4045, 4940
Bsp119I TTCGAA 3 cut(s) 70, 944, 5243
Bsp1286I GDGCHC 2 cut(s) 2830, 4470
Bsp13I TCCGGA 1 cut(s) 3928
Bsp1407I TGTACA 2 cut(s) 1199, 2297
Bsp19I CCATGG 3 cut(s) 1187, 1679, 3988
BspANI GGCC 9 cut(s) 2155, 2233, 2249, 2803, 2814, 4560, 4702, 4769, 4997
BspCNI CTCAG 9 cut(s) 346, 736, 753, 943, 964, 1435, 1888, 2012, 3564
BspEI TCCGGA 1 cut(s) 3928
BspHI TCATGA 2 cut(s) 655, 2434
BspLI GGNNCC 9 cut(s) 27, 1341, 1593, 1994, 2829, 4146, 4641, 4998, 5190
BspMI ACCTGC 4 cut(s) 4156, 4298, 5176, 5515
BspPI GGATC 6 cut(s) 1334, 1347, 1470, 1847, 2999, 4001
BspQI GCTCTTC 1 cut(s) 4284
BspT104I TTCGAA 3 cut(s) 70, 944, 5243
BspT107I GGYRCC 2 cut(s) 25, 4144
BspTNI GGTCTC 3 cut(s) 1604, 2596, 4398
BsrBI CCGCTC 1 cut(s) 4243
BsrDI GCAATG 1 cut(s) 5593
BsrFI RCCGGY 1 cut(s) 2814
BsrGI TGTACA 2 cut(s) 1199, 2297
BsrI ACTGG 3 cut(s) 1887, 3732, 4334
BssAI RCCGGY 1 cut(s) 2814
BssNAI GTATAC 1 cut(s) 3798
BssNI GRCGYC 1 cut(s) 2126
BssSI CACGAG 1 cut(s) 2130
Bst1107I GTATAC 1 cut(s) 3798
Bst2BI CACGAG 1 cut(s) 2130
Bst2UI CCWGG 7 cut(s) 1024, 1072, 1345, 2017, 3009, 4766, 5322
Bst4CI ACNGT 7 cut(s) 376, 995, 1698, 1966, 2549, 3653, 4827
Bst6I CTCTTC 3 cut(s) 2602, 4172, 4284
BstACI GRCGYC 1 cut(s) 2126
BstAPI GCANNNNNTGC 1 cut(s) 4313
BstAUI TGTACA 2 cut(s) 1199, 2297
BstBAI YACGTR 5 cut(s) 1825, 1838, 2789, 2799, 5027
BstBI TTCGAA 3 cut(s) 70, 944, 5243
BstC8I GCNNGC 5 cut(s) 409, 2816, 3212, 3631, 4995
BstDSI CCRYGG 4 cut(s) 1187, 1679, 3988, 4965
BstEII GGTNACC 2 cut(s) 989, 2681
BstH2I RGCGCY 1 cut(s) 2821
BstHHI GCGC 2 cut(s) 2288, 2820
BstMAI GTCTC 6 cut(s) 1604, 2596, 3579, 4398, 5129, 5607
BstMCI CGRYCG 2 cut(s) 2548, 5479
BstNI CCWGG 7 cut(s) 1024, 1072, 1345, 2017, 3009, 4766, 5322
BstNSI RCATGY 9 cut(s) 125, 303, 1513, 1865, 1975, 2286, 2298, 3218, 5372
BstPI GGTNACC 2 cut(s) 989, 2681
BstSCI CCNGG 8 cut(s) 1022, 1070, 1343, 2015, 3007, 4684, 4764, 5320
BstSFI CTRYAG 3 cut(s) 444, 816, 3843
BstSLI GKGCMC 1 cut(s) 4470
BstSNI TACGTA 1 cut(s) 1838
BstV1I GCAGC 6 cut(s) 646, 2664, 2667, 3935, 3954, 4327
BstV2I GAAGAC 2 cut(s) 184, 4929
BstX2I RGATCY 4 cut(s) 730, 1339, 1475, 4006
BstXI CCANNNNNNTGG 2 cut(s) 1733, 4777
BstYI RGATCY 4 cut(s) 730, 1339, 1475, 4006
BstZ17I GTATAC 1 cut(s) 3798
BsuI GTATCC 1 cut(s) 657
BsuRI GGCC 9 cut(s) 2155, 2233, 2249, 2803, 2814, 4560, 4702, 4769, 4997
BtgI CCRYGG 4 cut(s) 1187, 1679, 3988, 4965
BtrI CACGTC 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 1880
BveI ACCTGC 4 cut(s) 4156, 4298, 5176, 5515
Cac8I GCNNGC 5 cut(s) 409, 2816, 3212, 3631, 4995
CaiI CAGNNNCTG 3 cut(s) 526, 1882, 4340
CciI TCATGA 2 cut(s) 655, 2434
CfoI GCGC 2 cut(s) 2288, 2820
Cfr10I RCCGGY 1 cut(s) 2814
Cfr13I GGNCC 8 cut(s) 142, 1591, 1736, 2153, 2232, 2248, 2464, 4996
CseI GACGC 1 cut(s) 4756
DraI TTTAAA 2 cut(s) 609, 3142
EaeI YGGCCR 2 cut(s) 2801, 2812
Eam1104I CTCTTC 3 cut(s) 2602, 4172, 4284
EarI CTCTTC 3 cut(s) 2602, 4172, 4284
EciI GGCGGA 4 cut(s) 245, 2676, 4680, 4826
Eco105I TACGTA 1 cut(s) 1838
Eco24I GRGCYC 1 cut(s) 2830
Eco31I GGTCTC 3 cut(s) 1604, 2596, 4398
Eco32I GATATC 2 cut(s) 307, 4553
Eco47I GGWCC 4 cut(s) 142, 1591, 1736, 2464
Eco57I CTGAAG 4 cut(s) 53, 548, 1587, 1667
Eco72I CACGTG 2 cut(s) 1825, 2799
Eco88I CYCGRG 5 cut(s) 662, 2966, 3877, 4045, 4940
Eco91I GGTNACC 2 cut(s) 989, 2681
EcoO109I RGGNCCY 3 cut(s) 2232, 2464, 4996
EcoO65I GGTNACC 2 cut(s) 989, 2681
EcoRII CCWGG 7 cut(s) 1022, 1070, 1343, 2015, 3007, 4764, 5320
EcoRV GATATC 2 cut(s) 307, 4553
EcoT22I ATGCAT 3 cut(s) 1021, 5425, 5491
EcoT38I GRGCYC 1 cut(s) 2830
FalI AAGNNNNNCTT 4 cut(s) 837, 869, 3242, 3274
FaqI GGGAC 5 cut(s) 310, 2054, 2461, 4701, 4836
FauI CCCGC 1 cut(s) 2031
FauNDI CATATG 2 cut(s) 568, 1010
FbaI TGATCA 2 cut(s) 1333, 4495
FblI GTMKAC 4 cut(s) 327, 850, 3797, 3846
Fnu4HI GCNGC 8 cut(s) 635, 2653, 2656, 2659, 3924, 3968, 4341, 4991
FriOI GRGCYC 1 cut(s) 2830
Fsp4HI GCNGC 8 cut(s) 635, 2653, 2656, 2659, 3924, 3968, 4341, 4991
FspAI RTGCGCAY 1 cut(s) 2287
FspI TGCGCA 1 cut(s) 2287
GlaI GCGC 2 cut(s) 2287, 2819
GluI GCNGC 8 cut(s) 635, 2653, 2656, 2659, 3924, 3968, 4341, 4991
GsuI CTGGAG 3 cut(s) 1904, 3715, 5304
HaeII RGCGCY 1 cut(s) 2821
HaeIII GGCC 9 cut(s) 2155, 2233, 2249, 2803, 2814, 4560, 4702, 4769, 4997
HapII CCGG 6 cut(s) 1316, 2686, 2815, 2834, 3929, 4685
HgaI GACGC 1 cut(s) 4756
HhaI GCGC 2 cut(s) 2288, 2820
Hin1I GRCGYC 1 cut(s) 2126
Hin6I GCGC 2 cut(s) 2286, 2818
HinP1I GCGC 2 cut(s) 2286, 2818
HincII GTYRAC 7 cut(s) 117, 468, 851, 2451, 2712, 4324, 5530
HindII GTYRAC 7 cut(s) 117, 468, 851, 2451, 2712, 4324, 5530
HpaI GTTAAC 3 cut(s) 117, 468, 2712
HpaII CCGG 6 cut(s) 1316, 2686, 2815, 2834, 3929, 4685
Hpy99I CGWCG 3 cut(s) 1861, 2128, 2680
HpyCH4III ACNGT 7 cut(s) 376, 995, 1698, 1966, 2549, 3653, 4827
HpyCH4IV ACGT 9 cut(s) 174, 1815, 1824, 1837, 2126, 2586, 2788, 2798, 5026
HpySE526I ACGT 9 cut(s) 174, 1815, 1824, 1837, 2126, 2586, 2788, 2798, 5026
Hsp92I GRCGYC 1 cut(s) 2126
HspAI GCGC 2 cut(s) 2286, 2818
Kpn2I TCCGGA 1 cut(s) 3928
KroI GCCGGC 1 cut(s) 2814
KroNI GCCGGC 1 cut(s) 2816
Ksp22I TGATCA 2 cut(s) 1333, 4495
KspAI GTTAAC 3 cut(s) 117, 468, 2712
LguI GCTCTTC 1 cut(s) 4284
Lsp1109I GCAGC 6 cut(s) 646, 2664, 2667, 3935, 3954, 4327
MaeII ACGT 9 cut(s) 174, 1815, 1824, 1837, 2126, 2586, 2788, 2798, 5026
MbiI CCGCTC 1 cut(s) 4243
MfeI CAATTG 2 cut(s) 1268, 4508
MflI RGATCY 4 cut(s) 730, 1339, 1475, 4006
MhlI GDGCHC 2 cut(s) 2830, 4470
MlyI GAGTC 3 cut(s) 1273, 2465, 4683
MmeI TCCRAC 8 cut(s) 1076, 2434, 2568, 3212, 3342, 3864, 4375, 4589
Mph1103I ATGCAT 3 cut(s) 1021, 5425, 5491
MroI TCCGGA 1 cut(s) 3928
MroNI GCCGGC 1 cut(s) 2814
MroXI GAANNNNTTC 4 cut(s) 434, 4199, 4224, 5239
MslI CAYNNNNRTG 9 cut(s) 48, 1454, 1508, 2134, 2293, 2787, 2952, 3056, 5308
MspA1I CMGCKG 1 cut(s) 2658
MspI CCGG 6 cut(s) 1316, 2686, 2815, 2834, 3929, 4685
MspR9I CCNGG 8 cut(s) 1024, 1072, 1345, 2017, 3009, 4686, 4766, 5322
MunI CAATTG 2 cut(s) 1268, 4508
Mva1269I GAATGC 5 cut(s) 2200, 2654, 3761, 3906, 4184
MvaI CCWGG 7 cut(s) 1024, 1072, 1345, 2017, 3009, 4766, 5322
NaeI GCCGGC 1 cut(s) 2816
NciI CCSGG 1 cut(s) 4686
NcoI CCATGG 3 cut(s) 1187, 1679, 3988
NdeI CATATG 2 cut(s) 568, 1010
NgoMIV GCCGGC 1 cut(s) 2814
NlaIV GGNNCC 9 cut(s) 27, 1341, 1593, 1994, 2829, 4146, 4641, 4998, 5190
NmuCI GTSAC 7 cut(s) 376, 989, 1674, 2127, 2681, 3419, 5074
NsbI TGCGCA 1 cut(s) 2287
NsiI ATGCAT 3 cut(s) 1021, 5425, 5491
NspI RCATGY 9 cut(s) 125, 303, 1513, 1865, 1975, 2286, 2298, 3218, 5372
NspV TTCGAA 3 cut(s) 70, 944, 5243
OliI CACNNNNGTG 1 cut(s) 2134
PaeR7I CTCGAG 2 cut(s) 4045, 4940
PagI TCATGA 2 cut(s) 655, 2434
PaqCI CACCTGC 2 cut(s) 4156, 4298
PciI ACATGT 3 cut(s) 299, 1971, 2294
PciSI GCTCTTC 1 cut(s) 4284
PcsI WCGNNNNNNNCGW 3 cut(s) 180, 1821, 2132
PctI GAATGC 5 cut(s) 2200, 2654, 3761, 3906, 4184
PdiI GCCGGC 1 cut(s) 2816
PdmI GAANNNNTTC 4 cut(s) 434, 4199, 4224, 5239
PflFI GACNNNGTC 1 cut(s) 2455
PfoI TCCNGGA 1 cut(s) 4684
PkrI GCNGC 8 cut(s) 636, 2654, 2657, 2660, 3925, 3969, 4342, 4992
Ple19I CGATCG 1 cut(s) 5479
PleI GAGTC 3 cut(s) 1272, 2464, 4683
PmaCI CACGTG 2 cut(s) 1825, 2799
PmlI CACGTG 2 cut(s) 1825, 2799
PpsI GAGTC 3 cut(s) 1272, 2464, 4683
Ppu21I YACGTR 5 cut(s) 1825, 1838, 2789, 2799, 5027
PpuMI RGGWCCY 1 cut(s) 2464
PscI ACATGT 3 cut(s) 299, 1971, 2294
PshBI ATTAAT 2 cut(s) 458, 5397
PsiI TTATAA 1 cut(s) 5462
Psp5II RGGWCCY 1 cut(s) 2464
Psp6I CCWGG 7 cut(s) 1022, 1070, 1343, 2015, 3007, 4764, 5320
PspCI CACGTG 2 cut(s) 1825, 2799
PspEI GGTNACC 2 cut(s) 989, 2681
PspGI CCWGG 7 cut(s) 1022, 1070, 1343, 2015, 3007, 4764, 5320
PspN4I GGNNCC 9 cut(s) 27, 1341, 1593, 1994, 2829, 4146, 4641, 4998, 5190
PspPI GGNCC 8 cut(s) 142, 1591, 1736, 2153, 2232, 2248, 2464, 4996
PspPPI RGGWCCY 1 cut(s) 2464
PspXI VCTCGAGB 2 cut(s) 4045, 4940
PsrI GAACNNNNNNTAC 4 cut(s) 4830, 4862, 5020, 5052
PstNI CAGNNNCTG 3 cut(s) 526, 1882, 4340
PsuI RGATCY 4 cut(s) 730, 1339, 1475, 4006
PsyI GACNNNGTC 1 cut(s) 2455
PvuI CGATCG 1 cut(s) 5479
RseI CAYNNNNRTG 9 cut(s) 48, 1454, 1508, 2134, 2293, 2787, 2952, 3056, 5308
SalI GTCGAC 1 cut(s) 849
SapI GCTCTTC 1 cut(s) 4284
SatI GCNGC 8 cut(s) 635, 2653, 2656, 2659, 3924, 3968, 4341, 4991
Sau96I GGNCC 8 cut(s) 142, 1591, 1736, 2153, 2232, 2248, 2464, 4996
ScaI AGTACT 3 cut(s) 3650, 4038, 4734
SchI GAGTC 3 cut(s) 1273, 2465, 4683
ScrFI CCNGG 8 cut(s) 1024, 1072, 1345, 2017, 3009, 4686, 4766, 5322
SduI GDGCHC 2 cut(s) 2830, 4470
SfcI CTRYAG 3 cut(s) 444, 816, 3843
Sfr274I CTCGAG 2 cut(s) 4045, 4940
SfuI TTCGAA 3 cut(s) 70, 944, 5243
SinI GGWCC 4 cut(s) 142, 1591, 1736, 2464
SlaI CTCGAG 2 cut(s) 4045, 4940
SmiMI CAYNNNNRTG 9 cut(s) 48, 1454, 1508, 2134, 2293, 2787, 2952, 3056, 5308
SmlI CTYRAG 7 cut(s) 19, 1781, 2061, 2354, 2821, 4045, 4940
SmoI CTYRAG 7 cut(s) 19, 1781, 2061, 2354, 2821, 4045, 4940
SnaBI TACGTA 1 cut(s) 1838
SspI AATATT 5 cut(s) 1624, 2030, 2263, 2726, 4078
StyD4I CCNGG 8 cut(s) 1022, 1070, 1343, 2015, 3007, 4684, 4764, 5320
TaaI ACNGT 7 cut(s) 376, 995, 1698, 1966, 2549, 3653, 4827
TaiI ACGT 9 cut(s) 177, 1818, 1827, 1840, 2129, 2589, 2791, 2801, 5029
TatI WGTACW 8 cut(s) 371, 625, 919, 1199, 2297, 3648, 4036, 4732
TauI GCSGC 2 cut(s) 2661, 4993
TscAI CASTG 1 cut(s) 1887
TseFI GTSAC 7 cut(s) 376, 989, 1674, 2127, 2681, 3419, 5074
TseI GCWGC 6 cut(s) 634, 2652, 2655, 3923, 3967, 4340
Tsp45I GTSAC 7 cut(s) 376, 989, 1674, 2127, 2681, 3419, 5074
TspRI CASTG 1 cut(s) 1887
Tth111I GACNNNGTC 1 cut(s) 2455
VneI GTGCAC 1 cut(s) 4466
VpaK11BI GGWCC 4 cut(s) 142, 1591, 1736, 2464
VspI ATTAAT 2 cut(s) 458, 5397
XbaI TCTAGA 3 cut(s) 1792, 3409, 3434
XceI RCATGY 9 cut(s) 125, 303, 1513, 1865, 1975, 2286, 2298, 3218, 5372
XcmI CCANNNNNNNNNTGG 1 cut(s) 4629
XhoI CTCGAG 2 cut(s) 4045, 4940
XmaJI CCTAGG 2 cut(s) 3917, 5434
XmiI GTMKAC 4 cut(s) 327, 850, 3797, 3846
XmnI GAANNNNTTC 4 cut(s) 434, 4199, 4224, 5239
ZraI GACGTC 1 cut(s) 2127
ZrmI AGTACT 3 cut(s) 3650, 4038, 4734
Zsp2I ATGCAT 3 cut(s) 1021, 5425, 5491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.