pycom12420g00120

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012420
OG0000437
Physical Location & Seq
Forward (+)
88294 .. 89208
915 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12420g00120.1

Sequence Viewer

Length: 816 bp
ATGACTAGGAGTTCAAAACCTGTTCGTAAGCACATCCTTGACTTTGACGACGATTTTGAGCGGACCTTGAGGAAGCAAAAGAGTCAACAAAACCCTAACCCACCGTCTTTTAGCTCTACATCCGAGTTTGACGAAGGAGGTGAGGAAGGAGAGGCCACGGCACAGTTTGTGGAGGAAGTACAAACCATGGCCGTGGACAATCGAACCATCAAAGAGCTCTCTGCCTCAGGTATGGACAATGCCGTTCCTTTATGCATACAATACCCCGCGGCAGCTCAAGGTAAGACGGACGAATTTGAACTTAAGTCTAGTTTATTACATCATATTCCTAAATTCCATGGGCTGTCCATGGAAAATCCTAACAAACATCTTAGGGCCCGTTTGTTTGATAGGATTAAGGAGGACTGGACTGGACTGGACTATAGTCCCTTGTTTGGTCTGCTGGAGGATTTGGTCTTAACCAGGATCCCCGAAATTGGGCGGACTCGTAAGCCCAGAGAAAATCGCGAGGGTGACTTCTTGCTCGTCCTCCTTGCCCTCGCTCATCGTCGTCTTCCCTCATCGTCGTCTTGCCCTCGCTCATCGTTGTCTTCGTCACCCTCATCTCTGCGTCTTCCTCACCCTCATCTCTACGTCTTCCCTCATCTGCATTTGCTCGCCCTCATCTGCTCCGAGTTGTCTGGTAAACTTCGAATCTTCGATTTATTTCGTCATTTGTTTTGCAGATCTTTGAATCCTAGACCTCTGCACTTGAACAAATTAGGGTATATAATTTTGCGATTTGGGCTTTTCTGTGATGGTAAATCTGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 Cytochrom_B561

Protein Analysis

272

Amino Acids

30.87

Weight (kDa)

8.27

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06105
fragaria_vesca FvH4_1g09709 FvH4_4g02101 FvH4_4g08975 FvH4_6g23372 FvH4_6g39851 FvH4_c1g00300
malus_domestica MD01G1176100.v1.1 MD09G1284900.v1.1 MD15G1306600.v1.1
prunus_persica Prupe.1G227700_v2.0.a1
pyrus_communis pycom01g00630 pycom01g01020 pycom01g01690 pycom01g01760 pycom01g04190 pycom01g04400 pycom02g18130 pycom03g10160 pycom05g06370 pycom05g08930 pycom06g05590 pycom10g06320 pycom11g14330 pycom11g15320 pycom11g16240 pycom12416g00050 pycom12420g00010 pycom12420g00120 pycom12426g00180 pycom12426g00710 pycom12531g00010 pycom1256g00020 pycom1256g00050 pycom1341g00030 pycom1353g00030 pycom13g23070 pycom13g23210 pycom13g26260 pycom13g26370 pycom13g26470 pycom13g26980 pycom14g08550 pycom14g08850 pycom1534g00040 pycom15g28760 pycom15g31230 pycom1604g00040 pycom16g21900 pycom16g26030 pycom1739g00020 pycom1739g00050 pycom17g12970 pycom17g17960 pycom17g18300 pycom17g18650 pycom2172g00020 pycom2438g00020 pycom436g00370 pycom436g00390 pycom576g00050 pycom76g00020
rosa_chinensis RchiOBHm_Chr1g0320811 RchiOBHm_Chr2g0135321 RchiOBHm_Chr3g0485721 RchiOBHm_Chr7g0236571
rosa_multiflora Rmu_sc0000185.1_g000007 Rmu_sc0002094.1_g000030 Rmu_sc0002764.1_g000007 Rmu_sc0002809.1_g000014 Rmu_sc0003005.1_g000007 Rmu_sc0003798.1_g000005 Rmu_sc0004646.1_g000027 Rmu_sc0004665.1_g000009 Rmu_sc0004818.1_g000001 Rmu_sc0004988.1_g000031 Rmu_sc0006373.1_g000007 Rmu_sc0007142.1_g000015 Rmu_sc0009930.1_g000001 Rmu_sc0011451.1_g000001 Rmu_sc0020424.1_g000001 Rmu_sc0036944.1_g000001 Rmu_ssc0000062.1_g000010
rosa_roxburghii Rroxscaffold_2G00123930 Rroxscaffold_3G00231480 Rroxscaffold_6G00413880
rosa_rugosa Rorug04G0084800
rosa_samantha Rh3BG150500 Rh6DG232400
rosa_wichuraiana Rw2G018400 Rw3G027860 Rw4G021220 Rw6G020670 Rw6G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 61
AccII CGCG 2 cut(s) 269, 507
AciI CCGC 4 cut(s) 61, 267, 269, 481
AclWI GGATC 2 cut(s) 460, 473
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 2 cut(s) 293, 332
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 3 cut(s) 434, 476, 477
AflII CTTAAG 1 cut(s) 302
AgsI TTSAA 4 cut(s) 15, 299, 733, 754
AjnI CCWGG 1 cut(s) 461
AluBI AGCT 3 cut(s) 114, 217, 275
AluI AGCT 3 cut(s) 114, 217, 275
Alw21I GWGCWC 1 cut(s) 219
AlwI GGATC 2 cut(s) 460, 473
AoxI GGCC 3 cut(s) 153, 189, 375
ApaI GGGCCC 1 cut(s) 379
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 2 cut(s) 293, 332
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AspS9I GGNCC 3 cut(s) 63, 375, 376
AsuHPI GGTGA 4 cut(s) 152, 524, 588, 611
AsuII TTCGAA 1 cut(s) 691
AvaII GGWCC 1 cut(s) 63
AxyI CCTNAGG 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 379
BamHI GGATCC 1 cut(s) 465
BanII GRGCYC 2 cut(s) 219, 379
BbsI GAAGAC 4 cut(s) 545, 582, 605, 628
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 284
BccI CCATC 2 cut(s) 215, 791
BceAI ACGGC 3 cut(s) 174, 176, 227
BciT130I CCWGG 1 cut(s) 463
BfaI CTAG 3 cut(s) 6, 309, 738
BfmI CTRYAG 1 cut(s) 421
BfrI CTTAAG 1 cut(s) 302
BglII AGATCT 1 cut(s) 725
BisI GCNGC 2 cut(s) 270, 273
BlsI GCNGC 2 cut(s) 271, 274
Bme1390I CCNGG 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 63
BmgT120I GGNCC 3 cut(s) 63, 375, 376
BmiI GGNNCC 2 cut(s) 377, 467
BmrFI CCNGG 1 cut(s) 463
BoxI GACNNNNGTC 1 cut(s) 423
BpiI GAAGAC 4 cut(s) 545, 582, 605, 628
BpmI CTGGAG 1 cut(s) 464
Bpu14I TTCGAA 1 cut(s) 691
BpuEI CTTGAG 2 cut(s) 88, 261
BsaJI CCNNGG 6 cut(s) 156, 186, 192, 267, 337, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 476, 477
Bse1I ACTGG 3 cut(s) 410, 415, 420
Bse21I CCTNAGG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 6 cut(s) 156, 186, 192, 267, 337, 348
BseGI GGATG 2 cut(s) 33, 119
BseLI CCNNNNNNNGG 3 cut(s) 434, 476, 477
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 3 cut(s) 410, 415, 420
BseSI GKGCMC 1 cut(s) 379
BseXI GCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 2 cut(s) 269, 507
BshFI GGCC 3 cut(s) 155, 191, 377
BsiHKAI GWGCWC 1 cut(s) 219
BslFI GGGAC 1 cut(s) 411
BslI CCNNNNNNNGG 3 cut(s) 434, 476, 477
BsmFI GGGAC 1 cut(s) 411
BsnI GGCC 3 cut(s) 155, 191, 377
Bsp119I TTCGAA 1 cut(s) 691
Bsp120I GGGCCC 1 cut(s) 375
Bsp1286I GDGCHC 2 cut(s) 219, 379
Bsp143I GATC 2 cut(s) 465, 725
Bsp19I CCATGG 3 cut(s) 186, 337, 348
Bsp68I TCGCGA 1 cut(s) 507
BspACI CCGC 4 cut(s) 61, 267, 269, 481
BspANI GGCC 3 cut(s) 155, 191, 377
BspCNI CTCAG 1 cut(s) 239
BspFNI CGCG 2 cut(s) 269, 507
BspLI GGNNCC 2 cut(s) 377, 467
BspPI GGATC 2 cut(s) 460, 473
BspT104I TTCGAA 1 cut(s) 691
BspTI CTTAAG 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 61
BsrI ACTGG 3 cut(s) 410, 415, 420
BssECI CCNNGG 6 cut(s) 156, 186, 192, 267, 337, 348
BssMI GATC 2 cut(s) 465, 725
BssT1I CCWWGG 3 cut(s) 186, 337, 348
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 105, 165
BstAFI CTTAAG 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 691
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 2 cut(s) 226, 371
BstDSI CCRYGG 6 cut(s) 156, 186, 192, 267, 337, 348
BstF5I GGATG 2 cut(s) 33, 119
BstFNI CGCG 2 cut(s) 269, 507
BstKTI GATC 2 cut(s) 468, 728
BstMBI GATC 2 cut(s) 465, 725
BstMWI GCNNNNNNNGC 1 cut(s) 784
BstNI CCWGG 1 cut(s) 463
BstPAI GACNNNNGTC 1 cut(s) 423
BstSCI CCNGG 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 421
BstSLI GKGCMC 1 cut(s) 379
BstUI CGCG 2 cut(s) 269, 507
BstV1I GCAGC 1 cut(s) 284
BstV2I GAAGAC 4 cut(s) 545, 582, 605, 628
BstX2I RGATCY 2 cut(s) 465, 725
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 2 cut(s) 465, 725
Bsu36I CCTNAGG 1 cut(s) 226
BsuRI GGCC 3 cut(s) 155, 191, 377
BtgI CCRYGG 6 cut(s) 156, 186, 192, 267, 337, 348
BtsCI GGATG 2 cut(s) 33, 119
BtuMI TCGCGA 1 cut(s) 507
Cac8I GCNNGC 1 cut(s) 657
Cfr13I GGNCC 3 cut(s) 63, 375, 376
Cfr42I CCGCGG 1 cut(s) 270
CseI GACGC 1 cut(s) 599
Csp6I GTAC 1 cut(s) 179
CviAII CATG 3 cut(s) 187, 338, 349
CviJI RGCY 9 cut(s) 114, 155, 191, 217, 275, 343, 377, 493, 787
CviKI_1 RGCY 9 cut(s) 114, 155, 191, 217, 275, 343, 377, 493, 787
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 2 cut(s) 226, 371
DpnI GATC 2 cut(s) 467, 727
DpnII GATC 2 cut(s) 465, 725
EaeI YGGCCR 1 cut(s) 189
EciI GGCGGA 1 cut(s) 496
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 186, 337, 348
Eco24I GRGCYC 2 cut(s) 219, 379
Eco47I GGWCC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 217
Eco81I CCTNAGG 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 3 cut(s) 186, 337, 348
EcoT22I ATGCAT 1 cut(s) 257
EcoT38I GRGCYC 2 cut(s) 219, 379
ErhI CCWWGG 3 cut(s) 186, 337, 348
FaeI CATG 3 cut(s) 190, 341, 352
FaqI GGGAC 1 cut(s) 411
FatI CATG 3 cut(s) 186, 337, 348
FauI CCCGC 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 270, 273
FokI GGATG 2 cut(s) 20, 106
FriOI GRGCYC 2 cut(s) 219, 379
Fsp4HI GCNGC 2 cut(s) 270, 273
FspBI CTAG 3 cut(s) 6, 309, 738
GluI GCNGC 2 cut(s) 270, 273
GsuI CTGGAG 1 cut(s) 464
HaeIII GGCC 3 cut(s) 155, 191, 377
HgaI GACGC 1 cut(s) 599
Hin1II CATG 3 cut(s) 190, 341, 352
HincII GTYRAC 1 cut(s) 86
HindII GTYRAC 1 cut(s) 86
HinfI GANTC 4 cut(s) 82, 484, 693, 733
HphI GGTGA 4 cut(s) 152, 524, 588, 611
Hpy166II GTNNAC 3 cut(s) 86, 196, 686
Hpy188I TCNGA 3 cut(s) 124, 673, 808
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 3 cut(s) 86, 196, 686
Hpy99I CGWCG 3 cut(s) 53, 552, 568
HpyAV CCTTC 2 cut(s) 128, 140
HpyCH4III ACNGT 2 cut(s) 105, 165
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 4 cut(s) 255, 649, 723, 748
HpyF10VI GCNNNNNNNGC 1 cut(s) 784
HpyF3I CTNAG 2 cut(s) 226, 371
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 3 cut(s) 190, 341, 352
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 2 cut(s) 465, 725
LmnI GCTCC 1 cut(s) 674
Lsp1109I GCAGC 1 cut(s) 284
MaeI CTAG 3 cut(s) 6, 309, 738
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 2 cut(s) 512, 594
MalI GATC 2 cut(s) 467, 727
MbiI CCGCTC 1 cut(s) 61
MboI GATC 2 cut(s) 465, 725
MboII GAAGA 5 cut(s) 545, 582, 605, 628, 688
MflI RGATCY 2 cut(s) 465, 725
MhlI GDGCHC 2 cut(s) 219, 379
MluCI AATT 5 cut(s) 293, 332, 474, 758, 771
MlyI GAGTC 2 cut(s) 91, 478
Mph1103I ATGCAT 1 cut(s) 257
MseI TTAA 3 cut(s) 303, 396, 458
MslI CAYNNNNRTG 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 269
MspCI CTTAAG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 463
MvaI CCWGG 1 cut(s) 463
MvnI CGCG 2 cut(s) 269, 507
MwoI GCNNNNNNNGC 1 cut(s) 784
NcoI CCATGG 3 cut(s) 186, 337, 348
NdeII GATC 2 cut(s) 465, 725
NlaIII CATG 3 cut(s) 190, 341, 352
NlaIV GGNNCC 2 cut(s) 377, 467
NmuCI GTSAC 2 cut(s) 512, 594
NruI TCGCGA 1 cut(s) 507
NsiI ATGCAT 1 cut(s) 257
NspV TTCGAA 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 590
PfeI GAWTC 2 cut(s) 693, 733
PkrI GCNGC 2 cut(s) 271, 274
PleI GAGTC 2 cut(s) 90, 478
PpsI GAGTC 2 cut(s) 90, 478
PshAI GACNNNNGTC 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 461
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 2 cut(s) 377, 467
PspOMI GGGCCC 1 cut(s) 375
PspPI GGNCC 3 cut(s) 63, 375, 376
PsuI RGATCY 2 cut(s) 465, 725
RruI TCGCGA 1 cut(s) 507
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 191
SacI GAGCTC 1 cut(s) 219
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 3 cut(s) 303, 396, 458
SatI GCNGC 2 cut(s) 270, 273
Sau3AI GATC 2 cut(s) 465, 725
Sau96I GGNCC 3 cut(s) 63, 375, 376
SchI GAGTC 2 cut(s) 91, 478
ScrFI CCNGG 1 cut(s) 463
SduI GDGCHC 2 cut(s) 219, 379
SfcI CTRYAG 1 cut(s) 421
Sfr303I CCGCGG 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 691
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 3 cut(s) 67, 276, 302
SmoI CTYRAG 3 cut(s) 67, 276, 302
Sse9I AATT 5 cut(s) 293, 332, 474, 758, 771
SsiI CCGC 4 cut(s) 61, 267, 269, 481
SspMI CTAG 3 cut(s) 6, 309, 738
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 3 cut(s) 186, 337, 348
TaaI ACNGT 2 cut(s) 105, 165
TaiI ACGT 1 cut(s) 636
TaqI TCGA 3 cut(s) 202, 691, 699
TasI AATT 5 cut(s) 293, 332, 474, 758, 771
TatI WGTACW 1 cut(s) 178
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 2 cut(s) 693, 733
Tru1I TTAA 3 cut(s) 303, 396, 458
Tru9I TTAA 3 cut(s) 303, 396, 458
TseFI GTSAC 2 cut(s) 512, 594
TseI GCWGC 1 cut(s) 272
Tsp45I GTSAC 2 cut(s) 512, 594
TspGWI ACGGA 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 2 cut(s) 293, 332
XspI CTAG 3 cut(s) 6, 309, 738
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.