pycom01g04400

sterol 24-C-methyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000437
Physical Location & Seq
Forward (+)
3297880 .. 3298901
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g04400.1

Sequence Viewer

Length: 777 bp
ATGTGTGGAGAAGAACCCCTTGGCTATTCCATCATCCATTTTTTTCATCACATTCATATTTACATTTTTCCTTTAATTATATTCTGTCCATTTAATTTAATATCTCGTATTTGTTTTATTTTTGTATCTTTAAGCTTTTGGAGTCATAAACACTTTCAAATTCTCATAGTAGTCTTGTTAAGAGTCTTTACATTTAGTTTTTGTGTTTTCAAGTCAATTCAAAATAGATTAGCACCCCTAGTTAATCCCCGGTTAGAACGATCCCTACTTACATCATCACTACAATTGTCACAAATAGGGTTTAATTTGTGTGCGCATAAATCACGCGTCAATTTTGTGCAGGAAAGAGTTGCTCAGAAGCAGCTTTCTGTTCAGTTTATGTCTTCTAAAGAGCAGTTTGTTGATATTTTGACTAAAGGCCTGAGTTCTCCTTTGTTTCTTACACATTGTAACAATCTCATGCTCCGTGCATCGAAGTATAAGATGTTGCCATTTGTAATTAGATTAGAAGATCCGTTAGTTAAGGCAGTTAGTCTTGTGGTTATAGGAAACAAGATTCTTCTTCTCTCTCTAAATTCTCTCTCTCTCTCTCTCTATCTTTTCTTTATTCTTCTTCCTTGTTGTGTAAGATTGATCTTGCTTCGCCGTTGTAAAGGACGGTGTTTTATAGTGGTACTACTACAACAAAAAGCAACCCAAGTGATTTTCATTAACGAAAACAGAAGGAAATCCGTCTATATATGCAACATCAGCGAAGTAATAGGGTTGTCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25

Protein Analysis

259

Amino Acids

29.8

Weight (kDa)

9.83

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06105
fragaria_vesca FvH4_1g09709 FvH4_4g02101 FvH4_4g08975 FvH4_6g23372 FvH4_6g39851 FvH4_c1g00300
malus_domestica MD01G1176100.v1.1 MD09G1284900.v1.1 MD15G1306600.v1.1
prunus_persica Prupe.1G227700_v2.0.a1
pyrus_communis pycom01g00630 pycom01g01020 pycom01g01690 pycom01g01760 pycom01g04190 pycom01g04400 pycom02g18130 pycom03g10160 pycom05g06370 pycom05g08930 pycom06g05590 pycom10g06320 pycom11g14330 pycom11g15320 pycom11g16240 pycom12416g00050 pycom12420g00010 pycom12420g00120 pycom12426g00180 pycom12426g00710 pycom12531g00010 pycom1256g00020 pycom1256g00050 pycom1341g00030 pycom1353g00030 pycom13g23070 pycom13g23210 pycom13g26260 pycom13g26370 pycom13g26470 pycom13g26980 pycom14g08550 pycom14g08850 pycom1534g00040 pycom15g28760 pycom15g31230 pycom1604g00040 pycom16g21900 pycom16g26030 pycom1739g00020 pycom1739g00050 pycom17g12970 pycom17g17960 pycom17g18300 pycom17g18650 pycom2172g00020 pycom2438g00020 pycom436g00370 pycom436g00390 pycom576g00050 pycom76g00020
rosa_chinensis RchiOBHm_Chr1g0320811 RchiOBHm_Chr2g0135321 RchiOBHm_Chr3g0485721 RchiOBHm_Chr7g0236571
rosa_multiflora Rmu_sc0000185.1_g000007 Rmu_sc0002094.1_g000030 Rmu_sc0002764.1_g000007 Rmu_sc0002809.1_g000014 Rmu_sc0003005.1_g000007 Rmu_sc0003798.1_g000005 Rmu_sc0004646.1_g000027 Rmu_sc0004665.1_g000009 Rmu_sc0004818.1_g000001 Rmu_sc0004988.1_g000031 Rmu_sc0006373.1_g000007 Rmu_sc0007142.1_g000015 Rmu_sc0009930.1_g000001 Rmu_sc0011451.1_g000001 Rmu_sc0020424.1_g000001 Rmu_sc0036944.1_g000001 Rmu_ssc0000062.1_g000010
rosa_roxburghii Rroxscaffold_2G00123930 Rroxscaffold_3G00231480 Rroxscaffold_6G00413880
rosa_rugosa Rorug04G0084800
rosa_samantha Rh3BG150500 Rh6DG232400
rosa_wichuraiana Rw2G018400 Rw3G027860 Rw4G021220 Rw6G020670 Rw6G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 315
AccII CGCG 1 cut(s) 327
AclWI GGATC 2 cut(s) 255, 506
AcsI RAATTY 2 cut(s) 159, 574
AfaI GTAC 1 cut(s) 675
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 3 cut(s) 158, 211, 221
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 2 cut(s) 135, 364
AluI AGCT 2 cut(s) 135, 364
AlwI GGATC 2 cut(s) 255, 506
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 1 cut(s) 361
ApoI RAATTY 2 cut(s) 159, 574
AspLEI GCGC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 38
BceAI ACGGC 1 cut(s) 630
BcnI CCSGG 1 cut(s) 250
BfaI CTAG 1 cut(s) 239
BisI GCNGC 1 cut(s) 362
BlsI GCNGC 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 1 cut(s) 479
BpiI GAAGAC 1 cut(s) 375
BpuMI CCSGG 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 19, 248
BseDI CCNNGG 2 cut(s) 19, 248
BseGI GGATG 1 cut(s) 33
BseMII CTCAG 2 cut(s) 368, 413
BseXI GCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 1 cut(s) 420
BsiSI CCGG 1 cut(s) 250
BsnI GGCC 1 cut(s) 420
Bsp143I GATC 3 cut(s) 260, 511, 633
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 2 cut(s) 367, 414
BspFNI CGCG 1 cut(s) 327
BspPI GGATC 2 cut(s) 255, 506
BssECI CCNNGG 2 cut(s) 19, 248
BssMI GATC 3 cut(s) 260, 511, 633
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 660
BstDEI CTNAG 2 cut(s) 354, 422
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 1 cut(s) 327
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 3 cut(s) 263, 514, 636
BstMBI GATC 3 cut(s) 260, 511, 633
BstMWI GCNNNNNNNGC 1 cut(s) 750
BstSCI CCNGG 1 cut(s) 248
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuRI GGCC 1 cut(s) 420
BtsCI GGATG 1 cut(s) 33
CfoI GCGC 1 cut(s) 316
CseI GACGC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 674
CviAII CATG 1 cut(s) 460
CviJI RGCY 4 cut(s) 24, 135, 364, 420
CviKI_1 RGCY 4 cut(s) 24, 135, 364, 420
CviQI GTAC 1 cut(s) 674
DdeI CTNAG 2 cut(s) 354, 422
DpnI GATC 3 cut(s) 262, 513, 635
DpnII GATC 3 cut(s) 260, 511, 633
Eco130I CCWWGG 1 cut(s) 19
Eco147I AGGCCT 1 cut(s) 420
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 1 cut(s) 463
FalI AAGNNNNNCTT 1 cut(s) 35
FatI CATG 1 cut(s) 459
Fnu4HI GCNGC 1 cut(s) 362
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 1 cut(s) 362
FspAI RTGCGCAY 1 cut(s) 315
FspBI CTAG 1 cut(s) 239
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 1 cut(s) 315
GluI GCNGC 1 cut(s) 362
HaeIII GGCC 1 cut(s) 420
HapII CCGG 1 cut(s) 250
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 316
Hin1II CATG 1 cut(s) 463
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 3 cut(s) 142, 183, 556
HpaII CCGG 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 357
HpyAV CCTTC 1 cut(s) 717
HpyCH4III ACNGT 1 cut(s) 660
HpyCH4V TGCA 3 cut(s) 340, 470, 744
HpyF10VI GCNNNNNNNGC 1 cut(s) 750
HpyF3I CTNAG 2 cut(s) 354, 422
Hsp92II CATG 1 cut(s) 463
HspAI GCGC 1 cut(s) 314
Kzo9I GATC 3 cut(s) 260, 511, 633
LmnI GCTCC 1 cut(s) 468
LpnPI CCDG 3 cut(s) 263, 326, 434
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 1 cut(s) 479
MaeI CTAG 1 cut(s) 239
MaeIII GTNAC 2 cut(s) 288, 449
MalI GATC 3 cut(s) 262, 513, 635
MboI GATC 3 cut(s) 260, 511, 633
MboII GAAGA 7 cut(s) 23, 375, 521, 551, 554, 602, 605
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 1 cut(s) 511
MluCI AATT 9 cut(s) 75, 94, 159, 216, 284, 304, 331, 498, 574
MluI ACGCGT 1 cut(s) 325
MlyI GAGTC 2 cut(s) 151, 192
MseI TTAA 9 cut(s) 74, 93, 98, 131, 179, 243, 303, 522, 711
MspI CCGG 1 cut(s) 250
MspR9I CCNGG 1 cut(s) 250
MunI CAATTG 1 cut(s) 284
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 750
NciI CCSGG 1 cut(s) 250
NdeII GATC 3 cut(s) 260, 511, 633
NlaIII CATG 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 288
NsbI TGCGCA 1 cut(s) 315
PceI AGGCCT 1 cut(s) 420
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 1 cut(s) 363
PleI GAGTC 2 cut(s) 150, 191
PpsI GAGTC 2 cut(s) 150, 191
PsrI GAACNNNNNNTAC 2 cut(s) 249, 281
PsuI RGATCY 1 cut(s) 511
RsaI GTAC 1 cut(s) 675
RsaNI GTAC 1 cut(s) 674
SaqAI TTAA 9 cut(s) 74, 93, 98, 131, 179, 243, 303, 522, 711
SatI GCNGC 1 cut(s) 362
Sau3AI GATC 3 cut(s) 260, 511, 633
SchI GAGTC 2 cut(s) 151, 192
ScrFI CCNGG 1 cut(s) 250
SetI ASST 2 cut(s) 137, 366
SfaNI GCATC 1 cut(s) 479
Sse9I AATT 9 cut(s) 75, 94, 159, 216, 284, 304, 331, 498, 574
SseBI AGGCCT 1 cut(s) 420
SspMI CTAG 1 cut(s) 239
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 248
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 660
TaqI TCGA 1 cut(s) 473
TasI AATT 9 cut(s) 75, 94, 159, 216, 284, 304, 331, 498, 574
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 9 cut(s) 74, 93, 98, 131, 179, 243, 303, 522, 711
Tru9I TTAA 9 cut(s) 74, 93, 98, 131, 179, 243, 303, 522, 711
TseFI GTSAC 1 cut(s) 288
TseI GCWGC 1 cut(s) 361
Tsp45I GTSAC 1 cut(s) 288
TspDTI ATGAA 3 cut(s) 35, 44, 697
TspGWI ACGGA 3 cut(s) 455, 504, 721
XapI RAATTY 2 cut(s) 159, 574
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.