pycom1256g00050

protein kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001256
OG0000437
Physical Location & Seq
Forward (+)
37207 .. 41704
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom1256g00050.19

Sequence Viewer

Length: 3000 bp
ATGCATAAATATCATCCATATTTACAATTTGTTTTGTTTCCAAGTATTCATTTTAGTTTAAATTCGTCCAAATCCAATTCCCCCCTATTTCCATTAAATAGTGTTTTATATTGTGTTTTTATGTTTTTAAGTCAATTTAGAAGGTTTTACCATTCTACTACAATTGTCAAACGAGGGTTTAATTTGAGAAAAATAACTGGAAATTGGTTTATATGCATATATTATGTGTGGAGAAGGAACACCTCTAACTATCGTCAAAAACAATCCCCATTTCACTTAGTCTTCATATTTGAGTCAAAACTTGTTTTCCTTGAGTCTTTTGAGTCAAGTCAAGTTCTATTTTCGTCCAAAACACTTATTGAGTCTAAAGTTGAGTCTTTTTTATTATTGGTTGCTGTTTTAAGTTTAATCCCCGGTCTAGAACGATCCTTACTTACATATCTACTACAATTGTCACAAATAGGGTTTAATTTGTGTGTCAAGTTAATTTTCACATCACACACAATCTGCCATCATTCACAGAAACAAGCCCCTTTGCTGCAACCTTCAACCCATCTTTCCACCATTCTCCTGATAGGAGCATATTCATGCGACTTAATTAGCTTGTTCTCGTTCACTTTTGTGTATTTTCAGATTATAAAGCCAAATTGTGCAAGAAGATGCAAATTGGAGTCTTTTGGAAATTCCTATTCCAACGATGAATTCCTACTCCAACGAGGATTCCTACTCCAACAAGGATTCCTACTTGAAGTAGGAAAGTTAACCCTAGGTTTCCCCCACTTTGAAGGCCTTGTAGGCACTTTAGGACACAAAACAAAGGTCCTAGGGCCCTTAAACCCCTTTGCCATTGCCTCTCCCTTTCTTTCCTTCCCTTGCCGTGCAAGGGGGGGCAATTTTGTCTTAGCCGCACCTTGCACCTTTGACTTAATTTTAGTTAATAAAAATAAGCTAGGCATTCACTTTTCATTCACTTTTCATTCATTCTTTTCATATTCAGCCACATTCTCTCCCTTTGCCGTAGCCACCATCCCCCTTCTCCTTCAATCCGCAGCCACTCCACTAGTCCAGCCACCATTCCCAACCTTCCTCCACCACTCACACCCCTTAAAATCATCCCAAATCATCCAAAAACATCTCTGCCGCAGCAAGGAGAAGAAGAAAGAGGAGCTTAAACGCGCAGAAATTCAAGTGGATTCGTTGGGCGTTCTAGGTGTAATCAATCTTTTGTTCTTTATGTTTAATTTCAATTTTGTTTCTCTTTCTTCAAAACCATGTTTATGCTTGCAATATGAATTGATTACCTTTGGTTGGAATTTCATGAGTTGTGGATTCAATTTGTTTAACTGCTTGATTGATAACTTATTTGTGTATAATATAAGTGAGTTTCACCTAATAGTTACAAACTTATATTCACAAGTAGTGGAGGTCGCTTATAAACGATCGCTAACGAATGCCTCGTCAACACTTATAGTTTTCATGTTGACTTTTGGAGAATGTTTTGAATTGCGTATGCGCTTAACCCGGGGTGTTGAGTTTCATGATTCATCAAAAGACCAACTGAAAATCATCTTGTATGGAAGTGTGACATGTGTGGAGAAGAACCTTCTAGCTAGCATTCCATCCATATTTTCATCAAATTCATCTTTACAATCTGTTTTAATTACAATCTGTGCATTTTATTTAATTTCGTCAAAAACTAAATCCCCCTTTACTTTAGTGTCCAATTTATTATCACCCAATTTCGTCCAAATTAGTGTAAGTGCTCAAAATTGCCCAGATTGTATTTTTAAGGCATGTTTTAACCTTTCCCTCCTAATCCCCGGTCTAGAACGATCCCTACTTATTGATATACTACAATTGTCAAAAGAGGGTTTTAATTTGTGTGCTTATATATTCCGCATCAAATTTTTGGCGCCGTTGCCGGGGATTAGCAACTTTGCTAATCCCTTGGATTGTTATTTTCTGTTTATTTTATGTATGACTCGGAGTTCTCAATCGATTCGTGAGCATATCTTGGACTTTGACAGCGATTTTGAGAGGACTTTGAGACGAAAGAGAAAGCAACACGAATCAAATCCACAAAGCCCGGAACCTGACCTTGAAGAACAAGTCCTAGAAGAGGAAGAGGAGGCCACGGCAAGGGTTTTTGAAGAACCACAAGGCATGACCATGGACAACCGGACACTCAAGGAGCTTTCTGCTTCGGGATTGGATAATGCCGCACCTTTGTGTATCCAATATCCCCCACCTGCCCAAGGTAAGACCGAAGAGTTCGAGTTGAAGTCAAGTTTGCTCCACCACATTCCAAAATACCATGGGTTGTCCATGGAGGATCCGAACAAGCATCTGAAAGAATTCGAGGTGGTGTGTTCAAGCATGACTCCCGTCAACGTCGATGGAAGTATTTTGAAGATGAAGGCTTTTCCATTCTCTTTAATGGACAAAGCCAAAGATTGGTTGTACGAATTAGCTCCCGGAACTGTCACATCATGGGAGAGTATGAAGAGGGCATTTCTGGAGAAGTTTTTCCCAACTTCTCGAATCATCCTTTTACGTAAAAGGATAAGTGGAATTCAGCAAGAAGAAGGTGAGTCTTTTCCTACATATTATGAACGTTTTAAATCACTAGTTGCTTCTTGTCCACAACATCAGATGAAGGAGGAGTTACTTCTACAATACTTCTACGAGGGGCTCCTACCAATTGAACGTCAAATGCTCAATGCCTCGGCAAGTGGAGCATTGGTGGACAAGACACCCATGGTTGCCAAGGTCTTGATTGCTAACCGAGCGTTGAACGCTCAACAATACGAAGGAGTAGGCCAAAGAGGACCCCCACGGCAGCAAGTTCATGAGGGGCAAGAAAATCAAGCCAAAGCCATGCAAAACCAAGATAAGAGGGTGGAGCAATTGGAGAAACAAATTGGGCAGATTGCAGATTTTGTAGGGAAGTTTCGTGATCAAGGCCAACTTCCTAGTTCTACCATTCCAAACCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 RVT_1 Retrotrans_gag rve

Protein Analysis

1000

Amino Acids

113.74

Weight (kDa)

8.75

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06105
fragaria_vesca FvH4_1g09709 FvH4_4g02101 FvH4_4g08975 FvH4_6g23372 FvH4_6g39851 FvH4_c1g00300
malus_domestica MD01G1176100.v1.1 MD09G1284900.v1.1 MD15G1306600.v1.1
prunus_persica Prupe.1G227700_v2.0.a1
pyrus_communis pycom01g00630 pycom01g01020 pycom01g01690 pycom01g01760 pycom01g04190 pycom01g04400 pycom02g18130 pycom03g10160 pycom05g06370 pycom05g08930 pycom06g05590 pycom10g06320 pycom11g14330 pycom11g15320 pycom11g16240 pycom12416g00050 pycom12420g00010 pycom12420g00120 pycom12426g00180 pycom12426g00710 pycom12531g00010 pycom1256g00020 pycom1256g00050 pycom1341g00030 pycom1353g00030 pycom13g23070 pycom13g23210 pycom13g26260 pycom13g26370 pycom13g26470 pycom13g26980 pycom14g08550 pycom14g08850 pycom1534g00040 pycom15g28760 pycom15g31230 pycom1604g00040 pycom16g21900 pycom16g26030 pycom1739g00020 pycom1739g00050 pycom17g12970 pycom17g17960 pycom17g18300 pycom17g18650 pycom2172g00020 pycom2438g00020 pycom436g00370 pycom436g00390 pycom576g00050 pycom76g00020
rosa_chinensis RchiOBHm_Chr1g0320811 RchiOBHm_Chr2g0135321 RchiOBHm_Chr3g0485721 RchiOBHm_Chr7g0236571
rosa_multiflora Rmu_sc0000185.1_g000007 Rmu_sc0002094.1_g000030 Rmu_sc0002764.1_g000007 Rmu_sc0002809.1_g000014 Rmu_sc0003005.1_g000007 Rmu_sc0003798.1_g000005 Rmu_sc0004646.1_g000027 Rmu_sc0004665.1_g000009 Rmu_sc0004818.1_g000001 Rmu_sc0004988.1_g000031 Rmu_sc0006373.1_g000007 Rmu_sc0007142.1_g000015 Rmu_sc0009930.1_g000001 Rmu_sc0011451.1_g000001 Rmu_sc0020424.1_g000001 Rmu_sc0036944.1_g000001 Rmu_ssc0000062.1_g000010
rosa_roxburghii Rroxscaffold_2G00123930 Rroxscaffold_3G00231480 Rroxscaffold_6G00413880
rosa_rugosa Rorug04G0084800
rosa_samantha Rh3BG150500 Rh6DG232400
rosa_wichuraiana Rw2G018400 Rw3G027860 Rw4G021220 Rw6G020670 Rw6G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 638, 1434
AarI CACCTGC 1 cut(s) 2257
Acc36I ACCTGC 1 cut(s) 2257
AccB1I GGYRCC 1 cut(s) 1912
AccB7I CCANNNNNTGG 1 cut(s) 2454
AccII CGCG 1 cut(s) 1176
AciI CCGC 5 cut(s) 906, 1047, 1141, 1897, 2220
AclI AACGTT 1 cut(s) 2614
AclWI GGATC 4 cut(s) 420, 1827, 2327, 2340
AcsI RAATTY 9 cut(s) 61, 682, 701, 1182, 1312, 1636, 1904, 2354, 2571
AcyI GRCGYC 1 cut(s) 1913
AfaI GTAC 1 cut(s) 2462
AfiI CCNNNNNNNGG 8 cut(s) 883, 1147, 1308, 1922, 2119, 2139, 2255, 2454
AflIII ACRYGT 1 cut(s) 1586
AhlI ACTAGT 2 cut(s) 1060, 2626
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AleI CACNNNNGTG 2 cut(s) 620, 2227
AluBI AGCT 6 cut(s) 603, 949, 1168, 1610, 2194, 2471
AluI AGCT 6 cut(s) 603, 949, 1168, 1610, 2194, 2471
Alw21I GWGCWC 1 cut(s) 1765
Alw26I GTCTC 1 cut(s) 2041
AlwI GGATC 4 cut(s) 420, 1827, 2327, 2340
Ama87I CYCGRG 1 cut(s) 1521
AoxI GGCC 5 cut(s) 787, 827, 2130, 2818, 2962
ApaI GGGCCC 1 cut(s) 831
ApeKI GCWGC 4 cut(s) 538, 1049, 1143, 2839
ApoI RAATTY 9 cut(s) 61, 682, 701, 1182, 1312, 1636, 1904, 2354, 2571
ArsI GACNNNNNNTTYG 6 cut(s) 804, 836, 1442, 1474, 2015, 2047
Asp700I GAANNNNTTC 2 cut(s) 2354, 2525
AspA2I CCTAGG 2 cut(s) 766, 823
AspLEI GCGC 3 cut(s) 1178, 1515, 1915
AspS9I GGNCC 4 cut(s) 820, 827, 828, 2828
AsuC2I CCSGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
AsuHPI GGTGA 3 cut(s) 1379, 1725, 2600
AsuNHI GCTAGC 1 cut(s) 1610
AvaI CYCGRG 1 cut(s) 1521
AvaII GGWCC 2 cut(s) 820, 2828
AvrII CCTAGG 2 cut(s) 766, 823
BaeGI GKGCMC 1 cut(s) 831
BamHI GGATCC 1 cut(s) 2332
BanI GGYRCC 1 cut(s) 1912
BanII GRGCYC 2 cut(s) 831, 2694
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 1765
BbvI GCAGC 4 cut(s) 525, 1061, 1155, 2851
BccI CCATC 5 cut(s) 519, 561, 1034, 1627, 2390
BceAI ACGGC 5 cut(s) 861, 1001, 1900, 2151, 2852
BciVI GTATCC 1 cut(s) 2243
BclI TGATCA 1 cut(s) 2956
BcnI CCSGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
BcoDI GTCTC 1 cut(s) 2041
BcuI ACTAGT 2 cut(s) 1060, 2626
BfoI RGCGCY 1 cut(s) 1916
BfuAI ACCTGC 1 cut(s) 2257
BfuI GTATCC 1 cut(s) 2243
BglI GCCNNNNNGGC 1 cut(s) 795
BisI GCNGC 7 cut(s) 539, 906, 1050, 1141, 1144, 2220, 2840
BlnI CCTAGG 2 cut(s) 766, 823
BlsI GCNGC 7 cut(s) 540, 907, 1051, 1142, 1145, 2221, 2841
Bme1390I CCNGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
Bme18I GGWCC 2 cut(s) 820, 2828
BmeT110I CYCGRG 1 cut(s) 1521
BmgT120I GGNCC 4 cut(s) 820, 827, 828, 2828
BmiI GGNNCC 6 cut(s) 829, 1914, 2091, 2334, 2693, 2830
BmrFI CCNGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
BmsI GCATC 3 cut(s) 650, 1908, 2353
BmtI GCTAGC 1 cut(s) 1614
BoxI GACNNNNGTC 1 cut(s) 2384
BpiI GAAGAC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 2537
BpuEI CTTGAG 2 cut(s) 332, 2171
BpuMI CCSGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
Bsa29I ATCGAT 1 cut(s) 1997
BsaAI YACGTR 1 cut(s) 2555
BsaHI GRCGYC 1 cut(s) 1913
BsaWI WCCGGW 1 cut(s) 2178
BsaXI ACNNNNNCTCC 4 cut(s) 991, 1021, 2727, 2757
Bsc4I CCNNNNNNNGG 8 cut(s) 883, 1147, 1308, 1922, 2119, 2139, 2255, 2454
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 1 cut(s) 846
BseCI ATCGAT 1 cut(s) 1997
BseGI GGATG 6 cut(s) 13, 1026, 1112, 1122, 1619, 2544
BseLI CCNNNNNNNGG 8 cut(s) 883, 1147, 1308, 1922, 2119, 2139, 2255, 2454
BseMI GCAATG 1 cut(s) 846
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 3 cut(s) 1178, 2141, 2675
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 4 cut(s) 525, 1061, 1155, 2851
Bsh1236I CGCG 1 cut(s) 1176
Bsh1285I CGRYCG 1 cut(s) 1442
BshFI GGCC 5 cut(s) 789, 829, 2132, 2820, 2964
BshNI GGYRCC 1 cut(s) 1912
BshVI ATCGAT 1 cut(s) 1997
BsiEI CGRYCG 1 cut(s) 1442
BsiHKAI GWGCWC 1 cut(s) 1765
BsiHKCI CYCGRG 1 cut(s) 1521
BsiSI CCGG 7 cut(s) 414, 1522, 1821, 1922, 2087, 2179, 2475
BslI CCNNNNNNNGG 8 cut(s) 883, 1147, 1308, 1922, 2119, 2139, 2255, 2454
BsmAI GTCTC 1 cut(s) 2041
BsmBI CGTCTC 1 cut(s) 2041
BsmI GAATGC 3 cut(s) 954, 1456, 1614
BsnI GGCC 5 cut(s) 789, 829, 2132, 2820, 2964
BsoBI CYCGRG 1 cut(s) 1521
Bsp120I GGGCCC 1 cut(s) 827
Bsp1286I GDGCHC 3 cut(s) 831, 1765, 2694
Bsp143I GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
Bsp19I CCATGG 4 cut(s) 2169, 2314, 2325, 2757
BspACI CCGC 5 cut(s) 906, 1047, 1141, 1897, 2220
BspANI GGCC 5 cut(s) 789, 829, 2132, 2820, 2964
BspDI ATCGAT 1 cut(s) 1997
BspFNI CGCG 1 cut(s) 1176
BspHI TCATGA 3 cut(s) 1317, 1537, 2848
BspLI GGNNCC 6 cut(s) 829, 1914, 2091, 2334, 2693, 2830
BspMI ACCTGC 1 cut(s) 2257
BspOI GCTAGC 1 cut(s) 1614
BspPI GGATC 4 cut(s) 420, 1827, 2327, 2340
BspT107I GGYRCC 1 cut(s) 1912
BsrDI GCAATG 1 cut(s) 846
BsrI ACTGG 1 cut(s) 202
BssMI GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
BssNI GRCGYC 1 cut(s) 1913
BssT1I CCWWGG 9 cut(s) 766, 823, 1947, 2169, 2254, 2314, 2325, 2757, 2766
Bst4CI ACNGT 1 cut(s) 2482
Bst6I CTCTTC 4 cut(s) 2112, 2118, 2262, 2498
BstACI GRCGYC 1 cut(s) 1913
BstBAI YACGTR 1 cut(s) 2555
BstC8I GCNNGC 2 cut(s) 1283, 1612
BstDEI CTNAG 2 cut(s) 277, 901
BstDSI CCRYGG 6 cut(s) 2133, 2169, 2314, 2325, 2757, 2834
BstENI CCTNNNNNAGG 2 cut(s) 2117, 2253
BstF5I GGATG 6 cut(s) 13, 1026, 1112, 1122, 1619, 2544
BstFNI CGCG 1 cut(s) 1176
BstH2I RGCGCY 1 cut(s) 1916
BstHHI GCGC 3 cut(s) 1178, 1515, 1915
BstKTI GATC 5 cut(s) 428, 1442, 1835, 2335, 2959
BstMAI GTCTC 1 cut(s) 2041
BstMBI GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
BstMCI CGRYCG 1 cut(s) 1442
BstMWI GCNNNNNNNGC 4 cut(s) 795, 2735, 2786, 2795
BstNSI RCATGY 2 cut(s) 1590, 1797
BstPAI GACNNNNGTC 1 cut(s) 2384
BstSCI CCNGG 7 cut(s) 412, 1520, 1521, 1819, 1921, 2085, 2473
BstSLI GKGCMC 1 cut(s) 831
BstSNI TACGTA 1 cut(s) 2555
BstUI CGCG 1 cut(s) 1176
BstV1I GCAGC 4 cut(s) 525, 1061, 1155, 2851
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 2332
BstYI RGATCY 1 cut(s) 2332
Bsu15I ATCGAT 1 cut(s) 1997
BsuI GTATCC 1 cut(s) 2243
BsuRI GGCC 5 cut(s) 789, 829, 2132, 2820, 2964
BsuTUI ATCGAT 1 cut(s) 1997
BtgI CCRYGG 6 cut(s) 2133, 2169, 2314, 2325, 2757, 2834
BtsCI GGATG 6 cut(s) 13, 1026, 1112, 1122, 1619, 2544
BveI ACCTGC 1 cut(s) 2257
Cac8I GCNNGC 2 cut(s) 1283, 1612
CciI TCATGA 3 cut(s) 1317, 1537, 2848
CfoI GCGC 3 cut(s) 1178, 1515, 1915
Cfr13I GGNCC 4 cut(s) 820, 827, 828, 2828
Cfr9I CCCGGG 1 cut(s) 1521
ClaI ATCGAT 1 cut(s) 1997
Csp6I GTAC 1 cut(s) 2461
CviQI GTAC 1 cut(s) 2461
DdeI CTNAG 2 cut(s) 277, 901
DinI GGCGCC 1 cut(s) 1914
DpnI GATC 5 cut(s) 427, 1441, 1834, 2334, 2958
DpnII GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
DraI TTTAAA 2 cut(s) 60, 2620
Eam1104I CTCTTC 4 cut(s) 2112, 2118, 2262, 2498
EarI CTCTTC 4 cut(s) 2112, 2118, 2262, 2498
Eco105I TACGTA 1 cut(s) 2555
Eco130I CCWWGG 9 cut(s) 766, 823, 1947, 2169, 2254, 2314, 2325, 2757, 2766
Eco147I AGGCCT 1 cut(s) 789
Eco24I GRGCYC 2 cut(s) 831, 2694
Eco47I GGWCC 2 cut(s) 820, 2828
Eco88I CYCGRG 1 cut(s) 1521
EcoNI CCTNNNNNAGG 2 cut(s) 2117, 2253
EcoO109I RGGNCCY 4 cut(s) 820, 827, 828, 2828
EcoRI GAATTC 3 cut(s) 701, 2354, 2571
EcoT14I CCWWGG 9 cut(s) 766, 823, 1947, 2169, 2254, 2314, 2325, 2757, 2766
EcoT22I ATGCAT 2 cut(s) 6, 218
EcoT38I GRGCYC 2 cut(s) 831, 2694
EgeI GGCGCC 1 cut(s) 1914
EheI GGCGCC 1 cut(s) 1914
ErhI CCWWGG 9 cut(s) 766, 823, 1947, 2169, 2254, 2314, 2325, 2757, 2766
Esp3I CGTCTC 1 cut(s) 2041
FalI AAGNNNNNCTT 4 cut(s) 1152, 1184, 2952, 2984
FbaI TGATCA 1 cut(s) 2956
Fnu4HI GCNGC 7 cut(s) 539, 906, 1050, 1141, 1144, 2220, 2840
FokI GGATG 5 cut(s) 1013, 1099, 1109, 1606, 2531
FriOI GRGCYC 2 cut(s) 831, 2694
Fsp4HI GCNGC 7 cut(s) 539, 906, 1050, 1141, 1144, 2220, 2840
GlaI GCGC 3 cut(s) 1177, 1514, 1914
GluI GCNGC 7 cut(s) 539, 906, 1050, 1141, 1144, 2220, 2840
GsuI CTGGAG 1 cut(s) 2537
HaeII RGCGCY 1 cut(s) 1916
HaeIII GGCC 5 cut(s) 789, 829, 2132, 2820, 2964
HapII CCGG 7 cut(s) 414, 1522, 1821, 1922, 2087, 2179, 2475
HhaI GCGC 3 cut(s) 1178, 1515, 1915
Hin1I GRCGYC 1 cut(s) 1913
Hin6I GCGC 3 cut(s) 1176, 1513, 1913
HinP1I GCGC 3 cut(s) 1176, 1513, 1913
HincII GTYRAC 4 cut(s) 762, 1461, 1482, 2389
HindII GTYRAC 4 cut(s) 762, 1461, 1482, 2389
HpaI GTTAAC 1 cut(s) 762
HpaII CCGG 7 cut(s) 414, 1522, 1821, 1922, 2087, 2179, 2475
HphI GGTGA 3 cut(s) 1379, 1725, 2600
Hpy166II GTNNAC 7 cut(s) 615, 762, 1461, 1482, 2389, 2642, 2746
Hpy188I TCNGA 5 cut(s) 633, 1986, 2337, 2349, 2652
Hpy8I GTNNAC 7 cut(s) 615, 762, 1461, 1482, 2389, 2642, 2746
Hpy99I CGWCG 1 cut(s) 2396
HpyCH4III ACNGT 1 cut(s) 2482
HpyCH4IV ACGT 4 cut(s) 2391, 2554, 2614, 2707
HpyF10VI GCNNNNNNNGC 4 cut(s) 795, 2735, 2786, 2795
HpyF3I CTNAG 2 cut(s) 277, 901
HpySE526I ACGT 4 cut(s) 2391, 2554, 2614, 2707
Hsp92I GRCGYC 1 cut(s) 1913
HspAI GCGC 3 cut(s) 1176, 1513, 1913
KasI GGCGCC 1 cut(s) 1912
Ksp22I TGATCA 1 cut(s) 2956
KspAI GTTAAC 1 cut(s) 762
Kzo9I GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
LmnI GCTCC 8 cut(s) 578, 1165, 2191, 2298, 2476, 2697, 2735, 2902
Lsp1109I GCAGC 4 cut(s) 525, 1061, 1155, 2851
LweI GCATC 3 cut(s) 650, 1908, 2353
MaeII ACGT 4 cut(s) 2391, 2554, 2614, 2707
MaeIII GTNAC 5 cut(s) 453, 1396, 1582, 2482, 2664
MalI GATC 5 cut(s) 427, 1441, 1834, 2334, 2958
MboI GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
MfeI CAATTG 5 cut(s) 162, 449, 1856, 2700, 2906
MflI RGATCY 1 cut(s) 2332
MhlI GDGCHC 3 cut(s) 831, 1765, 2694
Mly113I GGCGCC 1 cut(s) 1913
MlyI GAGTC 9 cut(s) 302, 323, 332, 371, 383, 680, 1975, 2374, 2600
MmeI TCCRAC 4 cut(s) 717, 736, 754, 1289
Mph1103I ATGCAT 2 cut(s) 6, 218
MroXI GAANNNNTTC 2 cut(s) 2354, 2525
MslI CAYNNNNRTG 5 cut(s) 586, 620, 1276, 2168, 2227
MspI CCGG 7 cut(s) 414, 1522, 1821, 1922, 2087, 2179, 2475
MspR9I CCNGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
MunI CAATTG 5 cut(s) 162, 449, 1856, 2700, 2906
Mva1269I GAATGC 3 cut(s) 954, 1456, 1614
MvnI CGCG 1 cut(s) 1176
MwoI GCNNNNNNNGC 4 cut(s) 795, 2735, 2786, 2795
NarI GGCGCC 1 cut(s) 1913
NciI CCSGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
NcoI CCATGG 4 cut(s) 2169, 2314, 2325, 2757
NdeII GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
NheI GCTAGC 1 cut(s) 1610
NlaIV GGNNCC 6 cut(s) 829, 1914, 2091, 2334, 2693, 2830
NmeAIII GCCGAG 1 cut(s) 2705
NmuCI GTSAC 3 cut(s) 453, 1582, 2482
NsiI ATGCAT 2 cut(s) 6, 218
NspI RCATGY 2 cut(s) 1590, 1797
OliI CACNNNNGTG 2 cut(s) 620, 2227
PagI TCATGA 3 cut(s) 1317, 1537, 2848
PaqCI CACCTGC 1 cut(s) 2257
PceI AGGCCT 1 cut(s) 789
PciI ACATGT 1 cut(s) 1586
PcsI WCGNNNNNNNCGW 1 cut(s) 1454
PctI GAATGC 3 cut(s) 954, 1456, 1614
PdmI GAANNNNTTC 2 cut(s) 2354, 2525
PfeI GAWTC 8 cut(s) 720, 738, 1193, 1329, 1541, 1999, 2069, 2541
PflMI CCANNNNNTGG 1 cut(s) 2454
PfoI TCCNGGA 1 cut(s) 2473
PkrI GCNGC 7 cut(s) 540, 907, 1051, 1142, 1145, 2221, 2841
Ple19I CGATCG 1 cut(s) 1442
PleI GAGTC 9 cut(s) 301, 322, 331, 370, 382, 679, 1975, 2374, 2599
PluTI GGCGCC 1 cut(s) 1916
PpsI GAGTC 9 cut(s) 301, 322, 331, 370, 382, 679, 1975, 2374, 2599
Ppu21I YACGTR 1 cut(s) 2555
PpuMI RGGWCCY 2 cut(s) 820, 2828
PscI ACATGT 1 cut(s) 1586
PshAI GACNNNNGTC 1 cut(s) 2384
PsiI TTATAA 2 cut(s) 638, 1434
Psp1406I AACGTT 1 cut(s) 2614
Psp5II RGGWCCY 2 cut(s) 820, 2828
PspN4I GGNNCC 6 cut(s) 829, 1914, 2091, 2334, 2693, 2830
PspOMI GGGCCC 1 cut(s) 827
PspPI GGNCC 4 cut(s) 820, 827, 828, 2828
PspPPI RGGWCCY 2 cut(s) 820, 2828
PsrI GAACNNNNNNTAC 4 cut(s) 414, 446, 1821, 1853
PsuI RGATCY 1 cut(s) 2332
PvuI CGATCG 1 cut(s) 1442
RsaI GTAC 1 cut(s) 2462
RsaNI GTAC 1 cut(s) 2461
RseI CAYNNNNRTG 5 cut(s) 586, 620, 1276, 2168, 2227
SatI GCNGC 7 cut(s) 539, 906, 1050, 1141, 1144, 2220, 2840
Sau3AI GATC 5 cut(s) 425, 1439, 1832, 2332, 2956
Sau96I GGNCC 4 cut(s) 820, 827, 828, 2828
SchI GAGTC 9 cut(s) 302, 323, 332, 371, 383, 680, 1975, 2374, 2600
ScrFI CCNGG 7 cut(s) 414, 1522, 1523, 1821, 1923, 2087, 2475
SduI GDGCHC 3 cut(s) 831, 1765, 2694
SfaNI GCATC 3 cut(s) 650, 1908, 2353
SfoI GGCGCC 1 cut(s) 1914
SinI GGWCC 2 cut(s) 820, 2828
SmaI CCCGGG 1 cut(s) 1523
SmiMI CAYNNNNRTG 5 cut(s) 586, 620, 1276, 2168, 2227
SmlI CTYRAG 2 cut(s) 311, 2186
SmoI CTYRAG 2 cut(s) 311, 2186
SnaBI TACGTA 1 cut(s) 2555
SpeI ACTAGT 2 cut(s) 1060, 2626
SseBI AGGCCT 1 cut(s) 789
SsiI CCGC 5 cut(s) 906, 1047, 1141, 1897, 2220
SspDI GGCGCC 1 cut(s) 1912
StuI AGGCCT 1 cut(s) 789
StyD4I CCNGG 7 cut(s) 412, 1520, 1521, 1819, 1921, 2085, 2473
StyI CCWWGG 9 cut(s) 766, 823, 1947, 2169, 2254, 2314, 2325, 2757, 2766
TaaI ACNGT 1 cut(s) 2482
TaiI ACGT 4 cut(s) 2394, 2557, 2617, 2710
TaqI TCGA 5 cut(s) 1997, 2274, 2358, 2394, 2539
TaqII GACCGA 1 cut(s) 2279
TauI GCSGC 3 cut(s) 908, 1143, 2222
TfiI GAWTC 8 cut(s) 720, 738, 1193, 1329, 1541, 1999, 2069, 2541
TseFI GTSAC 3 cut(s) 453, 1582, 2482
TseI GCWGC 4 cut(s) 538, 1049, 1143, 2839
Tsp45I GTSAC 3 cut(s) 453, 1582, 2482
TspMI CCCGGG 1 cut(s) 1521
Van91I CCANNNNNTGG 1 cut(s) 2454
VpaK11BI GGWCC 2 cut(s) 820, 2828
XagI CCTNNNNNAGG 2 cut(s) 2117, 2253
XapI RAATTY 9 cut(s) 61, 682, 701, 1182, 1312, 1636, 1904, 2354, 2571
XbaI TCTAGA 2 cut(s) 418, 1825
XceI RCATGY 2 cut(s) 1590, 1797
XmaI CCCGGG 1 cut(s) 1521
XmaJI CCTAGG 2 cut(s) 766, 823
XmnI GAANNNNTTC 2 cut(s) 2354, 2525
Zsp2I ATGCAT 2 cut(s) 6, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.