pycom436g00390

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_436
OG0000437
Physical Location & Seq
Forward (+)
425496 .. 430897
5402 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom436g00390.3

Sequence Viewer

Length: 3753 bp
ATGCAATTGGAAATCATTTTATTATGCAAGTATAACATGTATGGAGATGAACCCTTAGCCATTATATCATCCATCCATTCAATTCCATCAATTTCATCTTTACTATCTGTTTTTACTTACAATCTGTGCATTTATATCTTAGTTTGTGTTTTTAAGTGTTTGAATCAATTTATTTCCCAAATTCGTCCAAAATTCACCAAAGTGCTCAAAACTGCCCAGAAAGTGATTTTAAGGCATGTTTTAATTTACGTTTTTGAGTCAAGTTACAACTTAATTTCTCCAAATTTGTGTTTGTGCTCAAATCTGCCCACAAAGTGGTTTTTAGGCAGATTTGAGTGTGTTTGTGTTGTTTTGAGTCTTTTGGTTTGTCTTAGTGTTTTAAAGTTTAGTTTTGCATTCTTTGAGTCTAGTATAGTGTTTTATATTGTCCCCGGTCCAGAACGATCCCTACTTATACTTATACTACAATTGACAAAAAGAGGGTTTAATTTATGCGCGTATAAATCTCGCATCAAATTTTGGCGCCGTTGCCGGGGATTAGGAAATTTGCTAATTCCTTGGATTGTTTTATTTTCGTTTGTTTTTATTTTATTCCAGTTTCTTAATCTTGTTTTGTTTGTTGTTCTAGGTACTATGATTGGGTTCTTCGTCCGGCTGCAAGACGTAAAAGAAAGCGCTACTGATAGAAGCATATTTATGCGCCTTTTTTCGTGTGTTTATAGGTTCATATGGCTAAGGATGCAAAAGGATGCCTTTCGGAGCCTTTTGGAGCAAATTAGAGATTGGAATGGATTGCATATGCTTAAAGCCAAAACAAAGAGTTCACATTCATCACCAACACAACCATACATCCACACATTCTTTCTCCATCATTGCCGTGCAAAACACCATCATTGCAGCCACATGCACATTCCACTTAATCATTTCAGATTTCCACCATCAACAAAGAACCATTCATTAATCATTTACACTTTAATCATTCAACCACATTCACACATCACCCATTCACCTTTGTGCCATGCTTCTACCTTTGTTCAAGCCCTTCCACATAACCTGCCTTCATTCATTCAGAAAATGCATTCCTTGCCGTGGCTTACATGCACTTCCCACTACCTTTCATTTCAGCCAAATCACATGCATTCCCACATGCATTTCCACCCACATACACCCATAATCATTCAACACATAATCAACCCACATGCACCATTCAAACATAAACCCATTCACCACCAGAAAACAACCATGCCGTGGGTTCCCATTCCAGATTTCCAACATTCCCTTTTATTTTCTGTCAAAACACATGCATCCCCACCCTTTAATCACATGCATTTCTGCCACATTCATCATGTTCCCCCCATTTCACCTATAAATAAACATGCATTGCAGCCACAAAATACACACTCTTCCATTTCAGAAAACCATCTCTCATACACATTCATTCACGCCAGAAATTCCTCCCCTCTCTCTAGCACTACCATACTTCACCATTCTTCATATTTTCTGCCCAAAAACACTCCTTCATCCATAACCACCAAAACTCACCAAAACCCCTTGTGCCTCAAGATTGAGTTGTTGGAATTTTTAGGTGTACTTCGATTCTTGTTTTCAATATTTACTTTGTTATTACTCTCTAAGTTTGTTGTAATCATGAGTGGTTTGAAATATGAATTGATTTCTTCCAATTGTGATTTGTTAAGTTGTGATTGTATTTCAATTATCATGTTTATTAAAAACTTAAACTCGATGCTAGTTTATAAACGAGTTTCACCTAATCGTCACAAGTTTATAAACATGAGTAGTGAAGGTTTCATAGTTACAATTGCCTCGTCAACACTTATAATTTTCATAGAGCATAATGATCTCTCATTGTATCTCTTCTATGCATTCATCATCATAGTTAATGAAAAACAATTGGAGAATCGATTCATATATATAAATGTATCATGTGTGGAGAATGGACCTCTAACTAATCCATCCATCATCTTATTTCTCAAATCTCAAAACCCCCCTTTACTTTCTTGTTCCTTGGTGCTTAAAATCTGTTTAGTTTATGTTTTCAAGTGTTTTGATTCAATTTTATTTCCCAATTTCGTCCAAATTCACCAAAGTGCTCAAAACTGCCCAGAAAGTGATTTTAAGGCATTTAGTGTTTTAAAGTTTAGTTTTGCATTCTTTGAGTCTAGTATAGTGTTTTATATTGTGTTTTTACAACGATCCCTACTTACATACTTACTACAATCAATAAAAAGAGGGTTTAATTTATGCGCGTATAAATCTCGCATCAAATTTTGGCGCCGTTGCCGGGGATTAGCAAAATTGCTAATCTCTTGTGTTTTTGCCGTATGTTTTAGTGTAATTTACCTATGCATGACGCGGAGTTCTCAAACTGTGCATGCACATATCTTGGACTTTAACGACGATTTTGAACGTGATTTGAGAAGAAAGAGGAGGCATCCCGAACCTAGCGAACCTAGTGAACCTAGTGAACCTAGTTCAAGTTCAGAGGCCGAATCTGAGTTTGAAAGTGTGGAAGAAGAAGTTATGGCAGCGGACAATCGGACCATTAAAGAGCTTTCGGCCTCGGGGTTGGCCAATGCCGCACCATTATGCATTCAATACCCCGCGGCTGCCCAAGGCAAGACCGATGAATTCGAGTTGAAGTCAAGCTTGTTGCACCACATTCCGAAATACCATGGGCTTTCCATGGAAGATCCCAACAAGCATCTCAAGGAGTTCGAGGTTGTTTGTTCGAGCATGACACCCATAAATGTCGATGGGAACATTCTAATGATGAAAGCTTTTCCATTCTCTCTTGTGGAAAAAGCTAAGGATTGGCTTTATGAATTGGCACCCGGGACCGTTACTTCTTGGGAGAGCATGAAGAGAGCATTCTTGGAGAAGTTCTTCCCTACTTCAAGAGTCATTCTCTTGAGGAAGAAAATTAGCGGAATCCAACAAAGTCAAGGAGAATCTTTTCCGGCATACTACGAGCGTTTTAAAGCCCTTGTTGCATCATGTCCTCAACATCAAATGAAGGAGGAGCTTCTCCTTCAATATTTCTATGAGGGCCTTCTTCCCATTGAAAGGCAAATGCTCGATGCTTCGGCGGGAGGTGCATTGGTAGACAAAACTCCTATGGCAGCCAAGACCCTAATCGCAAACCGTGCTCTAAATGCACAACAATATGAAGGACTTGGGCAAAGGGACCCCCCACGGCAACACTTGAATGAGGTAAGTTCTCAATCCGATATTCAATCTCAGATAGCTAATCTTACTTCTATTGTGTCTCAGATGGCCGAAGGAATGAAGATACAAGGATCGATTGGGCAAGAAGATCAAACCAAAGCCATGCAAAACCAAGACAAAAGGGTGGATCACTTGGAGAAACAAATTGGGCAGATTGCGGAGTTTGTAGGACAATTCCGACACAAAGGCAAGCTCCCTAGCTCAACCATTGTCAATCCAAGGGGAGGATTCGAATCTGCCAAGGCAATCATGTTGAGAAGTGGTAAGGAGGTCGAAACCACCCCAAGCACGTTTAGGCAATCAAAAAAGGAGGAGAGTGAAAAGGACATTTTGGAAACCTTTAGGAAAGTTCAAGTCAACATTCCGCTCCTTGATGCAATCAAGCAAGTCCCTAAGTATGCCAAGTTCTTAAAAGAGCTTTGCACAACGAGGAAGAGGATTTCAAACAGGGAGGTGGTCCAGGGGCTTCGATTAATGTTATGCCATACTCTATTTATGCATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotrans_gag

Protein Analysis

1251

Amino Acids

143.6

Weight (kDa)

9.24

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06105
fragaria_vesca FvH4_1g09709 FvH4_4g02101 FvH4_4g08975 FvH4_6g23372 FvH4_6g39851 FvH4_c1g00300
malus_domestica MD01G1176100.v1.1 MD09G1284900.v1.1 MD15G1306600.v1.1
prunus_persica Prupe.1G227700_v2.0.a1
pyrus_communis pycom01g00630 pycom01g01020 pycom01g01690 pycom01g01760 pycom01g04190 pycom01g04400 pycom02g18130 pycom03g10160 pycom05g06370 pycom05g08930 pycom06g05590 pycom10g06320 pycom11g14330 pycom11g15320 pycom11g16240 pycom12416g00050 pycom12420g00010 pycom12420g00120 pycom12426g00180 pycom12426g00710 pycom12531g00010 pycom1256g00020 pycom1256g00050 pycom1341g00030 pycom1353g00030 pycom13g23070 pycom13g23210 pycom13g26260 pycom13g26370 pycom13g26470 pycom13g26980 pycom14g08550 pycom14g08850 pycom1534g00040 pycom15g28760 pycom15g31230 pycom1604g00040 pycom16g21900 pycom16g26030 pycom1739g00020 pycom1739g00050 pycom17g12970 pycom17g17960 pycom17g18300 pycom17g18650 pycom2172g00020 pycom2438g00020 pycom436g00370 pycom436g00390 pycom576g00050 pycom76g00020
rosa_chinensis RchiOBHm_Chr1g0320811 RchiOBHm_Chr2g0135321 RchiOBHm_Chr3g0485721 RchiOBHm_Chr7g0236571
rosa_multiflora Rmu_sc0000185.1_g000007 Rmu_sc0002094.1_g000030 Rmu_sc0002764.1_g000007 Rmu_sc0002809.1_g000014 Rmu_sc0003005.1_g000007 Rmu_sc0003798.1_g000005 Rmu_sc0004646.1_g000027 Rmu_sc0004665.1_g000009 Rmu_sc0004818.1_g000001 Rmu_sc0004988.1_g000031 Rmu_sc0006373.1_g000007 Rmu_sc0007142.1_g000015 Rmu_sc0009930.1_g000001 Rmu_sc0011451.1_g000001 Rmu_sc0020424.1_g000001 Rmu_sc0036944.1_g000001 Rmu_ssc0000062.1_g000010
rosa_roxburghii Rroxscaffold_2G00123930 Rroxscaffold_3G00231480 Rroxscaffold_6G00413880
rosa_rugosa Rorug04G0084800
rosa_samantha Rh3BG150500 Rh6DG232400
rosa_wichuraiana Rw2G018400 Rw3G027860 Rw4G021220 Rw6G020670 Rw6G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1755, 1787, 1838
Acc36I ACCTGC 1 cut(s) 1062
AccB1I GGYRCC 3 cut(s) 522, 2292, 2848
AccB7I CCANNNNNTGG 2 cut(s) 315, 1248
AccBSI CCGCTC 1 cut(s) 3613
AccI GTMKAC 1 cut(s) 3123
AccII CGCG 4 cut(s) 497, 2267, 2374, 2624
AciI CCGC 9 cut(s) 2374, 2549, 2598, 2622, 2624, 2946, 3107, 3404, 3611
AclWI GGATC 5 cut(s) 438, 2208, 2705, 3325, 3381
AcoI YGGCCR 2 cut(s) 2589, 3294
AcyI GRCGYC 2 cut(s) 523, 2293
AdeI CACNNNGTG 1 cut(s) 315
AfaI GTAC 2 cut(s) 631, 1590
AfeI AGCGCT 1 cut(s) 676
AfiI CCNNNNNNNGG 7 cut(s) 315, 532, 1089, 1248, 2302, 3084, 3470
AflIII ACRYGT 1 cut(s) 36
AjnI CCWGG 1 cut(s) 3705
AleI CACNNNNGTG 3 cut(s) 200, 1012, 2106
AluBI AGCT 9 cut(s) 2572, 2667, 2798, 2825, 3043, 3266, 3439, 3447, 3664
AluI AGCT 9 cut(s) 2572, 2667, 2798, 2825, 3043, 3266, 3439, 3447, 3664
Alw21I GWGCWC 4 cut(s) 207, 299, 2113, 3169
Alw26I GTCTC 1 cut(s) 3291
AlwI GGATC 5 cut(s) 438, 2208, 2705, 3325, 3381
Ama87I CYCGRG 2 cut(s) 2581, 2852
Aor51HI AGCGCT 1 cut(s) 676
AoxI GGCC 5 cut(s) 2505, 2577, 2589, 3067, 3294
ApeKI GCWGC 6 cut(s) 655, 897, 1384, 2546, 2627, 3140
AseI ATTAAT 2 cut(s) 959, 3719
Asp700I GAANNNNTTC 3 cut(s) 1922, 2903, 2973
AspLEI GCGC 6 cut(s) 497, 525, 677, 702, 2267, 2295
AspS9I GGNCC 7 cut(s) 434, 1958, 2559, 2856, 3067, 3205, 3703
AsuC2I CCSGG 5 cut(s) 432, 533, 2303, 2853, 2854
AsuII TTCGAA 1 cut(s) 3477
AvaI CYCGRG 2 cut(s) 2581, 2852
AvaII GGWCC 6 cut(s) 434, 1958, 2559, 2856, 3205, 3703
BalI TGGCCA 1 cut(s) 2591
BanI GGYRCC 3 cut(s) 522, 2292, 2848
Bbv12I GWGCWC 4 cut(s) 207, 299, 2113, 3169
BbvI GCAGC 6 cut(s) 642, 909, 1396, 2558, 2614, 3152
BceAI ACGGC 7 cut(s) 510, 861, 1072, 1231, 2280, 2324, 3230
BciT130I CCWGG 1 cut(s) 3707
BcnI CCSGG 5 cut(s) 432, 533, 2303, 2853, 2854
BcoDI GTCTC 1 cut(s) 3291
BfoI RGCGCY 3 cut(s) 526, 678, 2296
BfuAI ACCTGC 1 cut(s) 1062
BisI GCNGC 8 cut(s) 656, 898, 1385, 2547, 2598, 2625, 2628, 3141
BlsI GCNGC 8 cut(s) 657, 899, 1386, 2548, 2599, 2626, 2629, 3142
Bme1390I CCNGG 6 cut(s) 432, 533, 2303, 2853, 2854, 3707
Bme18I GGWCC 6 cut(s) 434, 1958, 2559, 2856, 3205, 3703
BmeT110I CYCGRG 2 cut(s) 2581, 2852
BmgT120I GGNCC 7 cut(s) 434, 1958, 2559, 2856, 3067, 3205, 3703
BmiI GGNNCC 8 cut(s) 524, 761, 1254, 2294, 2850, 2857, 3206, 3207
BmrFI CCNGG 6 cut(s) 432, 533, 2303, 2853, 2854, 3707
BplI GAGNNNNNCTC 2 cut(s) 2910, 2942
Bpu10I CCTNAGC 3 cut(s) 55, 734, 2826
Bpu14I TTCGAA 1 cut(s) 3477
BpuEI CTTGAG 3 cut(s) 1544, 2711, 2950
BpuMI CCSGG 5 cut(s) 432, 533, 2303, 2853, 2854
Bsa29I ATCGAT 2 cut(s) 1921, 3320
BsaBI GATNNNNATC 1 cut(s) 3478
BsaHI GRCGYC 2 cut(s) 523, 2293
Bsc4I CCNNNNNNNGG 7 cut(s) 315, 532, 1089, 1248, 2302, 3084, 3470
Bse1I ACTGG 1 cut(s) 595
Bse3DI GCAATG 3 cut(s) 871, 892, 1379
Bse8I GATNNNNATC 1 cut(s) 3478
BseBI CCWGG 1 cut(s) 3707
BseCI ATCGAT 2 cut(s) 1921, 3320
BseGI GGATG 9 cut(s) 68, 72, 744, 754, 849, 1304, 1520, 1973, 2452
BseJI GATNNNNATC 1 cut(s) 3478
BseLI CCNNNNNNNGG 7 cut(s) 315, 532, 1089, 1248, 2302, 3084, 3470
BseMI GCAATG 3 cut(s) 871, 892, 1379
BseMII CTCAG 3 cut(s) 2505, 3272, 3302
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 3 cut(s) 2461, 3053, 3572
BseXI GCAGC 6 cut(s) 642, 909, 1396, 2558, 2614, 3152
Bsh1236I CGCG 4 cut(s) 497, 2267, 2374, 2624
BshFI GGCC 5 cut(s) 2507, 2579, 2591, 3069, 3296
BshNI GGYRCC 3 cut(s) 522, 2292, 2848
BshVI ATCGAT 2 cut(s) 1921, 3320
BsiHKAI GWGCWC 4 cut(s) 207, 299, 2113, 3169
BsiHKCI CYCGRG 2 cut(s) 2581, 2852
BsiSI CCGG 6 cut(s) 432, 532, 652, 2302, 2853, 2978
BslFI GGGAC 4 cut(s) 413, 2869, 3218, 3620
BslI CCNNNNNNNGG 7 cut(s) 315, 532, 1089, 1248, 2302, 3084, 3470
BsmAI GTCTC 1 cut(s) 3291
BsmFI GGGAC 4 cut(s) 413, 2869, 3218, 3620
BsmI GAATGC 7 cut(s) 395, 1078, 1138, 1883, 2168, 2610, 2888
BsnI GGCC 5 cut(s) 2507, 2579, 2591, 3069, 3296
BsoBI CYCGRG 2 cut(s) 2581, 2852
Bsp119I TTCGAA 1 cut(s) 3477
Bsp1286I GDGCHC 4 cut(s) 207, 299, 2113, 3169
Bsp143I GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
Bsp19I CCATGG 2 cut(s) 2692, 2703
BspACI CCGC 9 cut(s) 2374, 2549, 2598, 2622, 2624, 2946, 3107, 3404, 3611
BspANI GGCC 5 cut(s) 2507, 2579, 2591, 3069, 3296
BspCNI CTCAG 3 cut(s) 2506, 3271, 3301
BspDI ATCGAT 2 cut(s) 1921, 3320
BspFNI CGCG 4 cut(s) 497, 2267, 2374, 2624
BspHI TCATGA 1 cut(s) 1647
BspLI GGNNCC 8 cut(s) 524, 761, 1254, 2294, 2850, 2857, 3206, 3207
BspMI ACCTGC 1 cut(s) 1062
BspPI GGATC 5 cut(s) 438, 2208, 2705, 3325, 3381
BspT104I TTCGAA 1 cut(s) 3477
BspT107I GGYRCC 3 cut(s) 522, 2292, 2848
BsrBI CCGCTC 1 cut(s) 3613
BsrDI GCAATG 3 cut(s) 871, 892, 1379
BsrI ACTGG 1 cut(s) 595
BssMI GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
BssNI GRCGYC 2 cut(s) 523, 2293
BssT1I CCWWGG 7 cut(s) 557, 2025, 2632, 2692, 2703, 3464, 3486
Bst2UI CCWGG 1 cut(s) 3707
Bst4CI ACNGT 3 cut(s) 2389, 2860, 3164
Bst6I CTCTTC 4 cut(s) 1408, 1880, 2876, 3674
BstACI GRCGYC 2 cut(s) 523, 2293
BstAPI GCANNNNNTGC 2 cut(s) 1084, 3164
BstBI TTCGAA 1 cut(s) 3477
BstC8I GCNNGC 2 cut(s) 2394, 3437
BstDSI CCRYGG 6 cut(s) 1088, 1247, 2622, 2692, 2703, 3212
BstF5I GGATG 9 cut(s) 68, 72, 744, 754, 849, 1304, 1520, 1973, 2452
BstFNI CGCG 4 cut(s) 497, 2267, 2374, 2624
BstH2I RGCGCY 3 cut(s) 526, 678, 2296
BstHHI GCGC 6 cut(s) 497, 525, 677, 702, 2267, 2295
BstKTI GATC 7 cut(s) 446, 1861, 2216, 2713, 3320, 3337, 3376
BstMAI GTCTC 1 cut(s) 3291
BstMBI GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
BstMWI GCNNNNNNNGC 7 cut(s) 739, 1084, 2597, 3008, 3113, 3164, 3173
BstNI CCWGG 1 cut(s) 3707
BstSCI CCNGG 6 cut(s) 430, 531, 2301, 2851, 2852, 3705
BstUI CGCG 4 cut(s) 497, 2267, 2374, 2624
BstV1I GCAGC 6 cut(s) 642, 909, 1396, 2558, 2614, 3152
BstX2I RGATCY 1 cut(s) 2710
BstYI RGATCY 1 cut(s) 2710
Bsu15I ATCGAT 2 cut(s) 1921, 3320
BsuRI GGCC 5 cut(s) 2507, 2579, 2591, 3069, 3296
BsuTUI ATCGAT 2 cut(s) 1921, 3320
BtgI CCRYGG 6 cut(s) 1088, 1247, 2622, 2692, 2703, 3212
BtsCI GGATG 9 cut(s) 68, 72, 744, 754, 849, 1304, 1520, 1973, 2452
BveI ACCTGC 1 cut(s) 1062
Cac8I GCNNGC 2 cut(s) 2394, 3437
CciI TCATGA 1 cut(s) 1647
CfoI GCGC 6 cut(s) 497, 525, 677, 702, 2267, 2295
Cfr13I GGNCC 7 cut(s) 434, 1958, 2559, 2856, 3067, 3205, 3703
Cfr42I CCGCGG 1 cut(s) 2625
Cfr9I CCCGGG 1 cut(s) 2852
ClaI ATCGAT 2 cut(s) 1921, 3320
CseI GACGC 1 cut(s) 2380
Csp6I GTAC 2 cut(s) 630, 1589
CviQI GTAC 2 cut(s) 630, 1589
DinI GGCGCC 2 cut(s) 524, 2294
DpnI GATC 7 cut(s) 445, 1860, 2215, 2712, 3319, 3336, 3375
DpnII GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
DraI TTTAAA 3 cut(s) 381, 2154, 2998
DraIII CACNNNGTG 1 cut(s) 315
EaeI YGGCCR 2 cut(s) 2589, 3294
Eam1104I CTCTTC 4 cut(s) 1408, 1880, 2876, 3674
EarI CTCTTC 4 cut(s) 1408, 1880, 2876, 3674
Eco130I CCWWGG 7 cut(s) 557, 2025, 2632, 2692, 2703, 3464, 3486
Eco47I GGWCC 6 cut(s) 434, 1958, 2559, 2856, 3205, 3703
Eco47III AGCGCT 1 cut(s) 676
Eco88I CYCGRG 2 cut(s) 2581, 2852
EcoO109I RGGNCCY 2 cut(s) 3067, 3205
EcoRI GAATTC 1 cut(s) 2648
EcoRII CCWGG 1 cut(s) 3705
EcoT14I CCWWGG 7 cut(s) 557, 2025, 2632, 2692, 2703, 3464, 3486
EgeI GGCGCC 2 cut(s) 524, 2294
EheI GGCGCC 2 cut(s) 524, 2294
ErhI CCWWGG 7 cut(s) 557, 2025, 2632, 2692, 2703, 3464, 3486
FalI AAGNNNNNCTT 4 cut(s) 737, 769, 2651, 2683
FaqI GGGAC 4 cut(s) 413, 2869, 3218, 3620
FauI CCCGC 2 cut(s) 2629, 3100
FauNDI CATATG 2 cut(s) 728, 798
FblI GTMKAC 1 cut(s) 3123
Fnu4HI GCNGC 8 cut(s) 656, 898, 1385, 2547, 2598, 2625, 2628, 3141
FokI GGATG 9 cut(s) 55, 59, 751, 761, 836, 1291, 1507, 1960, 2439
Fsp4HI GCNGC 8 cut(s) 656, 898, 1385, 2547, 2598, 2625, 2628, 3141
GlaI GCGC 6 cut(s) 496, 524, 676, 701, 2266, 2294
GluI GCNGC 8 cut(s) 656, 898, 1385, 2547, 2598, 2625, 2628, 3141
HaeII RGCGCY 3 cut(s) 526, 678, 2296
HaeIII GGCC 5 cut(s) 2507, 2579, 2591, 3069, 3296
HapII CCGG 6 cut(s) 432, 532, 652, 2302, 2853, 2978
HgaI GACGC 1 cut(s) 2380
HhaI GCGC 6 cut(s) 497, 525, 677, 702, 2267, 2295
Hin1I GRCGYC 2 cut(s) 523, 2293
Hin6I GCGC 6 cut(s) 495, 523, 675, 700, 2265, 2293
HinP1I GCGC 6 cut(s) 495, 523, 675, 700, 2265, 2293
HincII GTYRAC 2 cut(s) 1830, 3604
HindII GTYRAC 2 cut(s) 1830, 3604
HindIII AAGCTT 2 cut(s) 2665, 2796
HpaII CCGG 6 cut(s) 432, 532, 652, 2302, 2853, 2978
Hpy166II GTNNAC 7 cut(s) 824, 1589, 1830, 2477, 2486, 3124, 3604
Hpy188III TCNNGA 7 cut(s) 437, 1262, 1561, 1648, 2456, 2916, 2929
Hpy8I GTNNAC 7 cut(s) 824, 1589, 1830, 2477, 2486, 3124, 3604
Hpy99I CGWCG 1 cut(s) 2420
HpyAV CCTTC 9 cut(s) 1052, 1068, 1527, 1796, 3028, 3059, 3080, 3182, 3293
HpyCH4III ACNGT 3 cut(s) 2389, 2860, 3164
HpyCH4IV ACGT 4 cut(s) 249, 663, 2428, 3536
HpyF10VI GCNNNNNNNGC 7 cut(s) 739, 1084, 2597, 3008, 3113, 3164, 3173
HpySE526I ACGT 4 cut(s) 249, 663, 2428, 3536
Hsp92I GRCGYC 2 cut(s) 523, 2293
HspAI GCGC 6 cut(s) 495, 523, 675, 700, 2265, 2293
KasI GGCGCC 2 cut(s) 522, 2292
KflI GGGWCCC 1 cut(s) 3205
KspI CCGCGG 1 cut(s) 2625
Kzo9I GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
LmnI GCTCC 5 cut(s) 759, 769, 3040, 3444, 3618
Lsp1109I GCAGC 6 cut(s) 642, 909, 1396, 2558, 2614, 3152
MaeII ACGT 4 cut(s) 249, 663, 2428, 3536
MaeIII GTNAC 4 cut(s) 263, 1775, 1813, 2860
MalI GATC 7 cut(s) 445, 1860, 2215, 2712, 3319, 3336, 3375
MbiI CCGCTC 1 cut(s) 3613
MboI GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
MfeI CAATTG 5 cut(s) 5, 467, 1681, 1818, 1910
MflI RGATCY 1 cut(s) 2710
MhlI GDGCHC 4 cut(s) 207, 299, 2113, 3169
MlsI TGGCCA 1 cut(s) 2591
MluNI TGGCCA 1 cut(s) 2591
Mly113I GGCGCC 2 cut(s) 523, 2293
MlyI GAGTC 5 cut(s) 266, 364, 413, 2186, 2928
MmeI TCCRAC 4 cut(s) 1294, 1554, 2977, 3448
Mox20I TGGCCA 1 cut(s) 2591
MroXI GAANNNNTTC 3 cut(s) 1922, 2903, 2973
MscI TGGCCA 1 cut(s) 2591
MslI CAYNNNNRTG 8 cut(s) 200, 695, 876, 1012, 2106, 2605, 2786, 3225
Msp20I TGGCCA 1 cut(s) 2591
MspA1I CMGCKG 2 cut(s) 2549, 2624
MspI CCGG 6 cut(s) 432, 532, 652, 2302, 2853, 2978
MspR9I CCNGG 6 cut(s) 432, 533, 2303, 2853, 2854, 3707
MunI CAATTG 5 cut(s) 5, 467, 1681, 1818, 1910
Mva1269I GAATGC 7 cut(s) 395, 1078, 1138, 1883, 2168, 2610, 2888
MvaI CCWGG 1 cut(s) 3707
MvnI CGCG 4 cut(s) 497, 2267, 2374, 2624
MwoI GCNNNNNNNGC 7 cut(s) 739, 1084, 2597, 3008, 3113, 3164, 3173
NarI GGCGCC 2 cut(s) 523, 2293
NciI CCSGG 5 cut(s) 432, 533, 2303, 2853, 2854
NcoI CCATGG 2 cut(s) 2692, 2703
NdeI CATATG 2 cut(s) 728, 798
NdeII GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
NlaIV GGNNCC 8 cut(s) 524, 761, 1254, 2294, 2850, 2857, 3206, 3207
NmuCI GTSAC 1 cut(s) 1775
NspV TTCGAA 1 cut(s) 3477
OliI CACNNNNGTG 3 cut(s) 200, 1012, 2106
PaeI GCATGC 1 cut(s) 2396
PagI TCATGA 1 cut(s) 1647
PciI ACATGT 1 cut(s) 36
PctI GAATGC 7 cut(s) 395, 1078, 1138, 1883, 2168, 2610, 2888
PdmI GAANNNNTTC 3 cut(s) 1922, 2903, 2973
PflMI CCANNNNNTGG 2 cut(s) 315, 1248
PkrI GCNGC 8 cut(s) 657, 899, 1386, 2548, 2599, 2626, 2629, 3142
PleI GAGTC 5 cut(s) 265, 363, 412, 2185, 2927
PluTI GGCGCC 2 cut(s) 526, 2296
PpsI GAGTC 5 cut(s) 265, 363, 412, 2185, 2927
PpuMI RGGWCCY 1 cut(s) 3205
PscI ACATGT 1 cut(s) 36
PshBI ATTAAT 2 cut(s) 959, 3719
PsiI TTATAA 3 cut(s) 1755, 1787, 1838
Psp5II RGGWCCY 1 cut(s) 3205
Psp6I CCWGG 1 cut(s) 3705
PspGI CCWGG 1 cut(s) 3705
PspN4I GGNNCC 8 cut(s) 524, 761, 1254, 2294, 2850, 2857, 3206, 3207
PspPI GGNCC 7 cut(s) 434, 1958, 2559, 2856, 3067, 3205, 3703
PspPPI RGGWCCY 1 cut(s) 3205
PsrI GAACNNNNNNTAC 4 cut(s) 432, 464, 3635, 3667
PsuI RGATCY 1 cut(s) 2710
RsaI GTAC 2 cut(s) 631, 1590
RsaNI GTAC 2 cut(s) 630, 1589
RseI CAYNNNNRTG 8 cut(s) 200, 695, 876, 1012, 2106, 2605, 2786, 3225
SacII CCGCGG 1 cut(s) 2625
SatI GCNGC 8 cut(s) 656, 898, 1385, 2547, 2598, 2625, 2628, 3141
Sau3AI GATC 7 cut(s) 443, 1858, 2213, 2710, 3317, 3334, 3373
Sau96I GGNCC 7 cut(s) 434, 1958, 2559, 2856, 3067, 3205, 3703
SchI GAGTC 5 cut(s) 266, 364, 413, 2186, 2928
ScrFI CCNGG 6 cut(s) 432, 533, 2303, 2853, 2854, 3707
SduI GDGCHC 4 cut(s) 207, 299, 2113, 3169
SfoI GGCGCC 2 cut(s) 524, 2294
Sfr303I CCGCGG 1 cut(s) 2625
SfuI TTCGAA 1 cut(s) 3477
SgrBI CCGCGG 1 cut(s) 2625
SinI GGWCC 6 cut(s) 434, 1958, 2559, 2856, 3205, 3703
SmaI CCCGGG 1 cut(s) 2854
SmiMI CAYNNNNRTG 8 cut(s) 200, 695, 876, 1012, 2106, 2605, 2786, 3225
SmlI CTYRAG 3 cut(s) 1559, 2726, 2929
SmoI CTYRAG 3 cut(s) 1559, 2726, 2929
SphI GCATGC 1 cut(s) 2396
SsiI CCGC 9 cut(s) 2374, 2549, 2598, 2622, 2624, 2946, 3107, 3404, 3611
SspDI GGCGCC 2 cut(s) 522, 2292
SspI AATATT 2 cut(s) 1611, 3056
StyD4I CCNGG 6 cut(s) 430, 531, 2301, 2851, 2852, 3705
StyI CCWWGG 7 cut(s) 557, 2025, 2632, 2692, 2703, 3464, 3486
TaaI ACNGT 3 cut(s) 2389, 2860, 3164
TaiI ACGT 4 cut(s) 252, 666, 2431, 3539
TaqII GACCGA 1 cut(s) 2657
TatI WGTACW 1 cut(s) 1588
TauI GCSGC 2 cut(s) 2600, 2627
TseFI GTSAC 1 cut(s) 1775
TseI GCWGC 6 cut(s) 655, 897, 1384, 2546, 2627, 3140
Tsp45I GTSAC 1 cut(s) 1775
TspMI CCCGGG 1 cut(s) 2852
Van91I CCANNNNNTGG 2 cut(s) 315, 1248
VpaK11BI GGWCC 6 cut(s) 434, 1958, 2559, 2856, 3205, 3703
VspI ATTAAT 2 cut(s) 959, 3719
XmaI CCCGGG 1 cut(s) 2852
XmiI GTMKAC 1 cut(s) 3123
XmnI GAANNNNTTC 3 cut(s) 1922, 2903, 2973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.