pycom01g01760

bile acid:sodium symporter activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000437
Physical Location & Seq
Forward (+)
1611565 .. 1613099
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g01760.1

Sequence Viewer

Length: 1260 bp
ATGATTTATTGGAATAATAATTGGAGATCGATTTGTTTGCATATGTTTCGTCCAAAGTTGTTTAGAGTCTTTAAATCTGTTTGCTTTTACTTTAAATTCGTCAAAAACCACTTCTCCCTTTATATTTCGTGTCTTTTAGTTAGAATCTGTTTAAGTTGGTTTCCTTTTAGTGTTTTGAGTCAACATAAGTCTAGAATTCGTCCAAATCATCCTTTAGTTCCTTTGGTCATTTTTGAGTCATTAGAGTCTAGTTTTGTGTTATTAAGTCTAGATCCCTACTTTCATATACTACAACTATCTAAAAAGGGTTTAATTTGTGTGCTAGTTTATATCACATCAAGGCGCCGTTGCCGAGGATTAGCAAAATTGCTAATCTCCCCTTTGTTTCTGGTTTTTCTTTTCTTGTGGTTTTTATTTTGTTTTTTATTTTTTGTTTACTTTGTTTTAGGTACTACTGTTTGTGCACATATATTGGAGTTTGACGGCGATTTTGAACGAACTTTGAAAAGGAAGAGGAAGCAACCAGAGCCCAATCCACCTAGTTCTAGCTCTGAATCTGAATTTGAAGAAGAAGATGAGGAAGAACAGGACATGGAGGTGGACAATGAAACAATCAAGGAACTTTCAGCCTCGGGATTGGACAATGCCGTGCCCATTTGCATTCAATATCCTAGGGCAGCTCAAGGCAAGACCGATGAGTTTGAATTGAAGTCAAGCTTGCTGCATCACATTCCCAAATATCATGGGCTGTCCATGGAAGATCGATACAAGCATTTGAAGGAATTTGAAGTGGTATGTTCGACCATGACACCAATCAACGTGGATGCCAATATTTTGAAGATGAAAGCCTTTCCATTCTCTCTTTTGGAAAAGGCTAAGGATTATATGAAGAAAGCATTCTTAGATAAATTCTTCCCAACTTCAAGAGTCATTCTTTTGAGGAAGAGAATGAGTGGAATTCAACAAGGCCAAGGAGAATCTTTTCCAGCATATTATGAGTGTTTCAAGACTTTAGTTGAGTCATGCCCACAACATCAAATGAAGGAAGAGCTTCTAATTCAATATTTCTACGAGGGACTCCTTCCAATTGAAAGACAAATGCTAAATGCTTCGATCCTTATAGCCGATCGAGCCTTGAATGCACAACAATATGAGGGAGTTGGACAAAGAGACCGACCACGGCAAACCGAAGTGAATGAGGTAAGTGCAATTTCCAAAATTCAATCTCAATTGGCTAATCTCACTGTTCTTGTGTCATAG

Protein Analysis

420

Amino Acids

48.97

Weight (kDa)

8.48

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 296 - 361 9.4e-11 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06105
fragaria_vesca FvH4_1g09709 FvH4_4g02101 FvH4_4g08975 FvH4_6g23372 FvH4_6g39851 FvH4_c1g00300
malus_domestica MD01G1176100.v1.1 MD09G1284900.v1.1 MD15G1306600.v1.1
prunus_persica Prupe.1G227700_v2.0.a1
pyrus_communis pycom01g00630 pycom01g01020 pycom01g01690 pycom01g01760 pycom01g04190 pycom01g04400 pycom02g18130 pycom03g10160 pycom05g06370 pycom05g08930 pycom06g05590 pycom10g06320 pycom11g14330 pycom11g15320 pycom11g16240 pycom12416g00050 pycom12420g00010 pycom12420g00120 pycom12426g00180 pycom12426g00710 pycom12531g00010 pycom1256g00020 pycom1256g00050 pycom1341g00030 pycom1353g00030 pycom13g23070 pycom13g23210 pycom13g26260 pycom13g26370 pycom13g26470 pycom13g26980 pycom14g08550 pycom14g08850 pycom1534g00040 pycom15g28760 pycom15g31230 pycom1604g00040 pycom16g21900 pycom16g26030 pycom1739g00020 pycom1739g00050 pycom17g12970 pycom17g17960 pycom17g18300 pycom17g18650 pycom2172g00020 pycom2438g00020 pycom436g00370 pycom436g00390 pycom576g00050 pycom76g00020
rosa_chinensis RchiOBHm_Chr1g0320811 RchiOBHm_Chr2g0135321 RchiOBHm_Chr3g0485721 RchiOBHm_Chr7g0236571
rosa_multiflora Rmu_sc0000185.1_g000007 Rmu_sc0002094.1_g000030 Rmu_sc0002764.1_g000007 Rmu_sc0002809.1_g000014 Rmu_sc0003005.1_g000007 Rmu_sc0003798.1_g000005 Rmu_sc0004646.1_g000027 Rmu_sc0004665.1_g000009 Rmu_sc0004818.1_g000001 Rmu_sc0004988.1_g000031 Rmu_sc0006373.1_g000007 Rmu_sc0007142.1_g000015 Rmu_sc0009930.1_g000001 Rmu_sc0011451.1_g000001 Rmu_sc0020424.1_g000001 Rmu_sc0036944.1_g000001 Rmu_ssc0000062.1_g000010
rosa_roxburghii Rroxscaffold_2G00123930 Rroxscaffold_3G00231480 Rroxscaffold_6G00413880
rosa_rugosa Rorug04G0084800
rosa_samantha Rh3BG150500 Rh6DG232400
rosa_wichuraiana Rw2G018400 Rw3G027860 Rw4G021220 Rw6G020670 Rw6G037970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AclWI GGATC 2 cut(s) 266, 1108
AcsI RAATTY 7 cut(s) 95, 195, 560, 782, 908, 957, 1218
AcyI GRCGYC 1 cut(s) 343
AfaI GTAC 1 cut(s) 451
AluBI AGCT 4 cut(s) 549, 680, 717, 1051
AluI AGCT 4 cut(s) 549, 680, 717, 1051
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 1 cut(s) 1164
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 2 cut(s) 266, 1108
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 1 cut(s) 967
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 677, 721
ApoI RAATTY 7 cut(s) 95, 195, 560, 782, 908, 957, 1218
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
Asp700I GAANNNNTTC 3 cut(s) 896, 981, 1050
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 1 cut(s) 345
AvaI CYCGRG 1 cut(s) 631
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 2 cut(s) 466, 654
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 689, 708
BceAI ACGGC 4 cut(s) 330, 499, 632, 1196
BcgI CGANNNNNNTGC 4 cut(s) 19, 29, 53, 63
BcoDI GTCTC 1 cut(s) 1164
BfaI CTAG 7 cut(s) 192, 249, 269, 323, 540, 546, 672
BfoI RGCGCY 1 cut(s) 346
BisI GCNGC 2 cut(s) 678, 722
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 2 cut(s) 679, 723
BmeT110I CYCGRG 1 cut(s) 631
BmiI GGNNCC 1 cut(s) 344
BmsI GCATC 2 cut(s) 733, 814
Bpu10I CCTNAGC 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 666
Bsa29I ATCGAT 2 cut(s) 29, 763
BsaHI GRCGYC 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 1164
BsaJI CCNNGG 6 cut(s) 352, 630, 671, 753, 970, 1178
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
BseCI ATCGAT 2 cut(s) 29, 763
BseDI CCNNGG 6 cut(s) 352, 630, 671, 753, 970, 1178
BseGI GGATG 2 cut(s) 208, 829
BseSI GKGCMC 2 cut(s) 466, 654
BseXI GCAGC 2 cut(s) 689, 708
Bsh1285I CGRYCG 1 cut(s) 1129
BshFI GGCC 1 cut(s) 969
BshNI GGYRCC 1 cut(s) 342
BshVI ATCGAT 2 cut(s) 29, 763
BsiEI CGRYCG 1 cut(s) 1129
BsiHKAI GWGCWC 1 cut(s) 466
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 1 cut(s) 1089
BsmAI GTCTC 1 cut(s) 1164
BsmFI GGGAC 1 cut(s) 1089
BsmI GAATGC 3 cut(s) 660, 896, 1144
BsnI GGCC 1 cut(s) 969
Bso31I GGTCTC 1 cut(s) 1164
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 3 cut(s) 466, 531, 654
Bsp143I GATC 5 cut(s) 26, 271, 760, 1113, 1126
Bsp19I CCATGG 1 cut(s) 753
BspANI GGCC 1 cut(s) 969
BspDI ATCGAT 2 cut(s) 29, 763
BspLI GGNNCC 1 cut(s) 344
BspPI GGATC 2 cut(s) 266, 1108
BspQI GCTCTTC 1 cut(s) 1041
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 1164
BssECI CCNNGG 6 cut(s) 352, 630, 671, 753, 970, 1178
BssMI GATC 5 cut(s) 26, 271, 760, 1113, 1126
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 3 cut(s) 671, 753, 970
Bst4CI ACNGT 2 cut(s) 457, 1246
Bst6I CTCTTC 3 cut(s) 506, 938, 1041
BstACI GRCGYC 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 719
BstDEI CTNAG 2 cut(s) 876, 901
BstDSI CCRYGG 2 cut(s) 753, 1178
BstF5I GGATG 2 cut(s) 208, 829
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 5 cut(s) 29, 274, 763, 1116, 1129
BstMAI GTCTC 1 cut(s) 1164
BstMBI GATC 5 cut(s) 26, 271, 760, 1113, 1126
BstMCI CGRYCG 1 cut(s) 1129
BstMWI GCNNNNNNNGC 3 cut(s) 526, 1130, 1139
BstSLI GKGCMC 2 cut(s) 466, 654
BstV1I GCAGC 2 cut(s) 689, 708
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
Bsu15I ATCGAT 2 cut(s) 29, 763
BsuRI GGCC 1 cut(s) 969
BsuTUI ATCGAT 2 cut(s) 29, 763
BtgI CCRYGG 2 cut(s) 753, 1178
BtsCI GGATG 2 cut(s) 208, 829
BtsIMutI CAGTG 1 cut(s) 1242
Cac8I GCNNGC 1 cut(s) 719
CfoI GCGC 1 cut(s) 345
ClaI ATCGAT 2 cut(s) 29, 763
Csp6I GTAC 1 cut(s) 450
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 5 cut(s) 592, 743, 754, 805, 1023
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 876, 901
DinI GGCGCC 1 cut(s) 344
DpnI GATC 5 cut(s) 28, 273, 762, 1115, 1128
DpnII GATC 5 cut(s) 26, 271, 760, 1113, 1126
DraI TTTAAA 2 cut(s) 73, 94
Eam1104I CTCTTC 3 cut(s) 506, 938, 1041
EarI CTCTTC 3 cut(s) 506, 938, 1041
Eco130I CCWWGG 3 cut(s) 671, 753, 970
Eco24I GRGCYC 1 cut(s) 531
Eco31I GGTCTC 1 cut(s) 1164
Eco88I CYCGRG 1 cut(s) 631
EcoRI GAATTC 2 cut(s) 195, 957
EcoT14I CCWWGG 3 cut(s) 671, 753, 970
EcoT38I GRGCYC 1 cut(s) 531
EgeI GGCGCC 1 cut(s) 344
EheI GGCGCC 1 cut(s) 344
ErhI CCWWGG 3 cut(s) 671, 753, 970
FaeI CATG 5 cut(s) 595, 746, 757, 808, 1026
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 1 cut(s) 1089
FatI CATG 5 cut(s) 591, 742, 753, 804, 1022
FauNDI CATATG 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 678, 722
FokI GGATG 2 cut(s) 195, 836
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 2 cut(s) 678, 722
FspBI CTAG 7 cut(s) 192, 249, 269, 323, 540, 546, 672
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 678, 722
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 1 cut(s) 969
HhaI GCGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 343
Hin1II CATG 5 cut(s) 595, 746, 757, 808, 1026
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HincII GTYRAC 1 cut(s) 182
HindII GTYRAC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 715
Hpy166II GTNNAC 4 cut(s) 182, 436, 464, 601
Hpy188I TCNGA 2 cut(s) 553, 559
Hpy188III TCNNGA 5 cut(s) 192, 269, 633, 924, 1006
Hpy8I GTNNAC 4 cut(s) 182, 436, 464, 601
HpyAV CCTTC 3 cut(s) 772, 1036, 1091
HpyCH4III ACNGT 2 cut(s) 457, 1246
HpyCH4IV ACGT 1 cut(s) 819
HpyCH4V TGCA 6 cut(s) 40, 464, 660, 724, 1142, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 526, 1130, 1139
HpyF3I CTNAG 2 cut(s) 876, 901
HpySE526I ACGT 1 cut(s) 819
Hsp92I GRCGYC 1 cut(s) 343
Hsp92II CATG 5 cut(s) 595, 746, 757, 808, 1026
HspAI GCGC 1 cut(s) 343
KasI GGCGCC 1 cut(s) 342
Kzo9I GATC 5 cut(s) 26, 271, 760, 1113, 1126
LguI GCTCTTC 1 cut(s) 1041
LpnPI CCDG 4 cut(s) 374, 537, 572, 999
Lsp1109I GCAGC 2 cut(s) 689, 708
LweI GCATC 2 cut(s) 733, 814
MaeI CTAG 7 cut(s) 192, 249, 269, 323, 540, 546, 672
MaeII ACGT 1 cut(s) 819
MalI GATC 5 cut(s) 28, 273, 762, 1115, 1128
MboI GATC 5 cut(s) 26, 271, 760, 1113, 1126
MfeI CAATTG 2 cut(s) 1086, 1229
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 3 cut(s) 466, 531, 654
Mly113I GGCGCC 1 cut(s) 343
MlyI GAGTC 7 cut(s) 75, 187, 245, 254, 936, 1028, 1071
MmeI TCCRAC 1 cut(s) 1141
MnlI CCTC 9 cut(s) 347, 507, 571, 589, 640, 933, 1066, 1147, 1192
MroXI GAANNNNTTC 3 cut(s) 896, 981, 1050
MseI TTAA 5 cut(s) 72, 93, 152, 263, 311
MslI CAYNNNNRTG 1 cut(s) 596
MunI CAATTG 2 cut(s) 1086, 1229
Mva1269I GAATGC 3 cut(s) 660, 896, 1144
MwoI GCNNNNNNNGC 3 cut(s) 526, 1130, 1139
NarI GGCGCC 1 cut(s) 343
NcoI CCATGG 1 cut(s) 753
NdeI CATATG 1 cut(s) 42
NdeII GATC 5 cut(s) 26, 271, 760, 1113, 1126
NlaIII CATG 5 cut(s) 595, 746, 757, 808, 1026
NlaIV GGNNCC 1 cut(s) 344
NmeAIII GCCGAG 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 1041
PctI GAATGC 3 cut(s) 660, 896, 1144
PdmI GAANNNNTTC 3 cut(s) 896, 981, 1050
PfeI GAWTC 3 cut(s) 144, 554, 977
PkrI GCNGC 2 cut(s) 679, 723
Ple19I CGATCG 1 cut(s) 1129
PleI GAGTC 7 cut(s) 74, 186, 244, 253, 935, 1027, 1071
PluTI GGCGCC 1 cut(s) 346
PpsI GAGTC 7 cut(s) 74, 186, 244, 253, 935, 1027, 1071
PspN4I GGNNCC 1 cut(s) 344
PsuI RGATCY 1 cut(s) 271
PvuI CGATCG 1 cut(s) 1129
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 5 cut(s) 72, 93, 152, 263, 311
SatI GCNGC 2 cut(s) 678, 722
Sau3AI GATC 5 cut(s) 26, 271, 760, 1113, 1126
SchI GAGTC 7 cut(s) 75, 187, 245, 254, 936, 1028, 1071
SduI GDGCHC 3 cut(s) 466, 531, 654
SetI ASST 9 cut(s) 451, 541, 551, 600, 682, 719, 822, 1053, 1203
SfaNI GCATC 2 cut(s) 733, 814
SfoI GGCGCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 681
SmoI CTYRAG 1 cut(s) 681
SspDI GGCGCC 1 cut(s) 342
SspI AATATT 2 cut(s) 832, 1064
SspMI CTAG 7 cut(s) 192, 249, 269, 323, 540, 546, 672
StyI CCWWGG 3 cut(s) 671, 753, 970
TaaI ACNGT 2 cut(s) 457, 1246
TaiI ACGT 1 cut(s) 822
TaqI TCGA 5 cut(s) 29, 763, 800, 1112, 1129
TaqII GACCGA 2 cut(s) 707, 1188
TfiI GAWTC 3 cut(s) 144, 554, 977
Tru1I TTAA 5 cut(s) 72, 93, 152, 263, 311
Tru9I TTAA 5 cut(s) 72, 93, 152, 263, 311
TscAI CASTG 1 cut(s) 1249
TseI GCWGC 2 cut(s) 677, 721
TspDTI ATGAA 5 cut(s) 272, 621, 857, 902, 1055
TspRI CASTG 1 cut(s) 1249
VneI GTGCAC 1 cut(s) 462
XapI RAATTY 7 cut(s) 95, 195, 560, 782, 908, 957, 1218
XbaI TCTAGA 2 cut(s) 191, 268
XmaJI CCTAGG 1 cut(s) 671
XmnI GAANNNNTTC 3 cut(s) 896, 981, 1050
XspI CTAG 7 cut(s) 192, 249, 269, 323, 540, 546, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.