MD00G1101400.v1.1

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000086
Physical Location & Seq
Reverse (-)
21480096 .. 21482303
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1101400.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGATTCCTCAAGATTGTTAATTCTCTTCACTGTTTCCATTCTCTTGGGCCTTCTTGCACCCGCCGTAGCCCAAGTCAATGACGCTGTATGCACTTATACCGCTGATTTCTGCCGGAGATGTTCCTACATTGGCACCTACGAAGACGGTGGCGCCTACCAAAAAATCCTCAACACCCTCCTCTCCTCTCTCTCTTCCAACAATCAAACCAACTCCGGCTTTTACAATTCATCGACGGGGCAAGACCCCTACAAAGTGAACGCAATTGCATTGTGTAGAGGAGATGTGTCCCTCAACGACTGTCACACTTGTGTCAACCAGTCTAGTTCCATTCTCTTCACGGATTGTTTGAATCAAGACGAAGCAATCATATGGGGGGAGCGTTGCATGGTGCGATACTCGAGCAAATCAATATTTGGTACTCAGGAGGATGAGCCTATTAAGTACGTTCCTAGCCTGAGTAACGCAACTGATCCCGAAGAATTTGATTTGGTGTTCAATCTCTTTTTGGATACTTTAACTGGGAAAGCTGCGTCGGGGGATTCTCTTAAAAAATTTGCAGCTGGACATTGCACTCCAGATTTGGGTAAGAAAAGCTGCAGTGATTGCCTCAAAGGGGCTATCTCAAAAATACCAGAGTGTTGTGGTGGAAAGCAAGGAGGCAGAGTTCTTAAGCCGAGTTGTAATTTGAGATTCGAGACAAATCTTTTCTACAACTTTACCGCCGATTCTCTAGTAACTCTCCCAGGAAAGAAGAGTACCATATCAAAAGCAGTCATCGCAATCATCATCACTGCTGTTGCGATTGTTTTTGTCGCTATGATTCTTATCGGAATATTTATTTCCTTAAGAATGAGGAGACGAAGGAAAAAACTAAAACTTAAAAATGATAATTCGGATGACATTGGACTGGTGGAATCGTTGCAATATGACTTCGAAACTATAAGATCTGCAACAGATGACTTCTCTGATGCAAATAAGCTTGGCCAAGGAGGATTTGGAGCTGTCTACAAGGGTAGGCTACCAAATGGAAAATATATAGCCGTGAAAAGACTATCTAAGAATTCTGAACAAGGTGATCGTGAATTTAAAACTGAAGTCACGTTAGTTTCTCAACTTCACCACCGGAATCTAGTAGGACTGCTAGGTTTTTGCTTGAAAGCAGGGGAAAGAATCCTCATTTACGAATACGTGCCCAACACAAGTCTTAATCACTTCATTTTTGATCCAATCAATCATGGACATTTGAATTGGGAAACACGTTACAAAATCATAAGAGGGATTTCTCGAGGGCTTCTTTACCTTCATGAAGATTCTTGTCCTCGAATCATTCACCGTGATCTTAAACCTAGCAACATTCTGTTAGATGAAGATATGAATCCTAAAATTGCAGACTTTGGCATGGCTCAATTGTTTATGATGGATCAAACTCTAGGAGATACTAAGACAATCATGGGGACCTATGGATATATGGCTCCAGAGTATGCGATTCATGGTAGTTTTTCTGTCAAATCGGATGTTTTTAGTTTTGGTGTGTTAGTCCTTGAGATCTTAAGCGGTAGAAAGATTACCAGTTTCCAAAGTGCCGAGAATGAAGAGGACCTTTTAACCTATGTAAGAATATACTCTTTTGCACATTATTTAACTCTTAGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

553

Amino Acids

61.29

Weight (kDa)

7.11

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 38 - 135 1e-22 Salt stress response/antifungal
Stress-antifung PF01657 155 - 234 2.7e-07 Salt stress response/antifungal
Pkinase PF00069 323 - 531 4.9e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 326 - 529 2.8e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21230 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21410 AT4G21410 AT4G21410 AT4G38830
fragaria_vesca FvH4_2g29861 FvH4_2g29861 FvH4_2g29870 FvH4_2g29870 FvH4_2g29870 FvH4_2g29871 FvH4_2g29871 FvH4_2g29875 FvH4_2g29878 FvH4_3g02833 FvH4_3g02833 FvH4_3g02834 FvH4_3g02870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g20010
malus_domestica MD00G1101400.v1.1 MD00G1101700.v1.1 MD01G1027900.v1.1 MD02G1101400.v1.1 MD05G1336800.v1.1 MD05G1337000.v1.1 MD05G1337300.v1.1 MD05G1337400.v1.1 MD05G1337500.v1.1 MD07G1014000.v1.1 MD08G1139800.v1.1 MD08G1139900.v1.1 MD08G1140000.v1.1 MD08G1140100.v1.1 MD08G1140200.v1.1 MD08G1140400.v1.1 MD10G1312200.v1.1 MD10G1312500.v1.1 MD15G1117400.v1.1
prunus_persica Prupe.1G174500_v2.0.a1 Prupe.1G468600_v2.0.a1 Prupe.1G469000_v2.0.a1 Prupe.1G469100_v2.0.a1 Prupe.1G469200_v2.0.a1 Prupe.1G469300_v2.0.a1 Prupe.4G027900_v2.0.a1 Prupe.4G028000_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028200_v2.0.a1 Prupe.4G028600_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028800_v2.0.a1 Prupe.4G100700_v2.0.a1 Prupe.6G183400_v2.0.a1 Prupe.6G183500_v2.0.a1 Prupe.6G183600_v2.0.a1 Prupe.6G184100_v2.0.a1 Prupe.6G184400_v2.0.a1 Prupe.6G184500_v2.0.a1
pyrus_communis pycom01g05620 pycom01g05630 pycom05g30820 pycom05g30840 pycom05g30860 pycom05g30880 pycom05g30890 pycom05g30900 pycom08g11780 pycom08g11850 pycom10g26340 pycom10g26350 pycom10g26360 pycom10g26400 pycom10g26410 pycom15g10580
rosa_chinensis RchiOBHm_Chr1g0313011 RchiOBHm_Chr1g0319461 RchiOBHm_Chr1g0319511 RchiOBHm_Chr2g0114611 RchiOBHm_Chr2g0114631 RchiOBHm_Chr2g0114691 RchiOBHm_Chr2g0114741 RchiOBHm_Chr2g0114751 RchiOBHm_Chr2g0114781 RchiOBHm_Chr2g0114791 RchiOBHm_Chr3g0494511 RchiOBHm_Chr3g0494521 RchiOBHm_Chr4g0413731 RchiOBHm_Chr5g0004271 RchiOBHm_Chr5g0004291 RchiOBHm_Chr5g0004301 RchiOBHm_Chr5g0004341 RchiOBHm_Chr5g0004351 RchiOBHm_Chr5g0004371 RchiOBHm_Chr5g0033301 RchiOBHm_Chr5g0033311 RchiOBHm_Chr5g0033341 RchiOBHm_Chr5g0033361 RchiOBHm_Chr5g0033391 RchiOBHm_Chr5g0033401 RchiOBHm_Chr5g0033431 RchiOBHm_Chr5g0033451 RchiOBHm_Chr5g0033461 RchiOBHm_Chr5g0033531 RchiOBHm_Chr5g0033541 RchiOBHm_Chr5g0033711 RchiOBHm_Chr5g0033851 RchiOBHm_Chr5g0033861 RchiOBHm_Chr5g0033871 RchiOBHm_Chr5g0054051 RchiOBHm_Chr5g0069631 RchiOBHm_Chr6g0265231 RchiOBHm_Chr6g0298941 RchiOBHm_Chr6g0298951 RchiOBHm_Chr6g0299001 RchiOBHm_Chr6g0299011 RchiOBHm_Chr6g0299021
rosa_laevigata RLG00000008198 RLG00000010335 RLG00000011468 RLG00000011471 RLG00000014070 RLG00000014071 RLG00000018116 RLG00000018125 RLG00000031219 RLG00000031220 RLG00000031221 RLG00000031222 RLG00000031224 RLG00000033469 RLG00000033471 RLG00000033473 RLG00000033476 RLG00000033477 RLG00000033479 RLG00000033480 RLG00000033483 RLG00000035638
rosa_multiflora Rmu_co8063382.1_g000001 Rmu_co8095246.1_g000001 Rmu_sc0000370.1_g000009 Rmu_sc0000370.1_g000019 Rmu_sc0000371.1_g000023 Rmu_sc0000371.1_g000036 Rmu_sc0000433.1_g000023 Rmu_sc0000433.1_g000024 Rmu_sc0000545.1_g000004 Rmu_sc0000981.1_g000001 Rmu_sc0001150.1_g000032 Rmu_sc0001242.1_g000004 Rmu_sc0001242.1_g000008 Rmu_sc0001522.1_g000035 Rmu_sc0002206.1_g000008 Rmu_sc0002206.1_g000021 Rmu_sc0002453.1_g000008 Rmu_sc0002453.1_g000018 Rmu_sc0003286.1_g000007 Rmu_sc0003286.1_g000013 Rmu_sc0004133.1_g000005 Rmu_sc0005660.1_g000005 Rmu_sc0005660.1_g000019 Rmu_sc0005660.1_g000025 Rmu_sc0005870.1_g000017 Rmu_sc0005870.1_g000022 Rmu_sc0006083.1_g000003 Rmu_sc0008092.1_g000016 Rmu_sc0008210.1_g000007 Rmu_sc0009715.1_g000001 Rmu_sc0011893.1_g000003 Rmu_sc0011893.1_g000004 Rmu_sc0015325.1_g000002 Rmu_sc0029922.1_g000001 Rmu_ssc0000359.1_g000017 Rmu_ssc0000424.1_g000009
rosa_roxburghii Rroxscaffold_175G00432170 Rroxscaffold_1G00022110 Rroxscaffold_1G00046520 Rroxscaffold_1G00046550 Rroxscaffold_1G00046600 Rroxscaffold_1G00046650 Rroxscaffold_1G00046680 Rroxscaffold_1G00071130 Rroxscaffold_1G00071380 Rroxscaffold_1G00071410 Rroxscaffold_2G00130310 Rroxscaffold_2G00130320 Rroxscaffold_2G00130330 Rroxscaffold_4G00328340 Rroxscaffold_4G00328360 Rroxscaffold_4G00328370 Rroxscaffold_5G00357650 Rroxscaffold_7G00156890 Rroxscaffold_7G00169080 Rroxscaffold_7G00169090 Rroxscaffold_7G00169100 Rroxscaffold_7G00169170 Rroxscaffold_7G00169190
rosa_rugosa Rorug01G0027900 Rorug01G0130600 Rorug02G0190700 Rorug04G0411000 Rorug04G0411000 Rorug04G0411200 Rorug04G0411300 Rorug04G0411400 Rorug04G0411500 Rorug05G0139300 Rorug05G0139500 Rorug05G0139800 Rorug05G0139900 Rorug05G0187000 Rorug05G0187000 Rorug05G0187000 Rorug05G0398900 Rorug06G0034600 Rorug06G0283900 Rorug06G0284000 Rorug06G0284100 Rorug06G0394700
rosa_samantha Rh1BG036800 Rh1CG040700 Rh1CG040800 Rh1DG000200 Rh1DG028900 Rh1DG090600 Rh2AG248900 Rh2CG252600 Rh2CG253100 Rh2CG253500 Rh2CG253600 Rh2CG253900 Rh4DG177700 Rh5CG041500 Rh5CG041700 Rh5CG042100 Rh5CG260400 Rh5CG260500 Rh5CG260600 Rh5CG260900 Rh5CG261100 Rh5DG037300 Rh5DG037400 Rh5DG037500 Rh5DG236800 Rh5DG236900 Rh5DG237100 Rh5DG237500 Rh5DG237600 Rh5DG237700 Rh5DG241100 Rh6CG134500 Rh6CG409700 Rh6CG409800 Rh6CG410100 Rh6DG120900 Rh6DG396500 Rh6DG396600 Rh6DG396700 Rh6DG514400
rosa_wichuraiana Rw1G003200 Rw1G003210 Rw1G003230 Rw2G019350 Rw4G015240 Rw5G003500 Rw5G003520 Rw5G003530 Rw5G003690 Rw5G003720 Rw5G003730 Rw5G021090 Rw5G021100 Rw5G021110 Rw5G021120 Rw5G021130 Rw6G011870 Rw6G034630 Rw7G038700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 134, 152
AccI GTMKAC 1 cut(s) 1008
AciI CCGC 4 cut(s) 63, 102, 723, 1557
AclWI GGATC 3 cut(s) 467, 1220, 1431
AcoI YGGCCR 1 cut(s) 985
AcsI RAATTY 4 cut(s) 482, 554, 1063, 1085
AcuI CTGAAG 1 cut(s) 1116
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 421, 446, 760
AfiI CCNNNNNNNGG 2 cut(s) 584, 616
AflII CTTAAG 3 cut(s) 671, 847, 1552
AflIII ACRYGT 1 cut(s) 1259
AgsI TTSAA 4 cut(s) 352, 499, 1159, 1249
AjnI CCWGG 1 cut(s) 745
AleI CACNNNNGTG 1 cut(s) 309
AloI GAACNNNNNNTCC 2 cut(s) 651, 683
AluBI AGCT 5 cut(s) 530, 563, 597, 982, 1004
AluI AGCT 5 cut(s) 530, 563, 597, 982, 1004
Alw26I GTCTC 2 cut(s) 692, 853
AlwI GGATC 3 cut(s) 467, 1220, 1431
Ama87I CYCGRG 2 cut(s) 400, 1287
AoxI GGCC 2 cut(s) 49, 985
ApeKI GCWGC 3 cut(s) 530, 560, 597
ApoI RAATTY 4 cut(s) 482, 554, 1063, 1085
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 3 cut(s) 49, 1458, 1600
AsuHPI GGTGA 3 cut(s) 1088, 1112, 1325
AsuII TTCGAA 1 cut(s) 936
AvaI CYCGRG 2 cut(s) 400, 1287
AvaII GGWCC 2 cut(s) 1458, 1600
BaeGI GKGCMC 1 cut(s) 1197
BalI TGGCCA 1 cut(s) 987
BanI GGYRCC 2 cut(s) 134, 152
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 517, 572, 584
BccI CCATC 1 cut(s) 1414
BceAI ACGGC 2 cut(s) 50, 1028
BciT130I CCWGG 1 cut(s) 747
BciVI GTATCC 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 692, 853
BfaI CTAG 7 cut(s) 324, 453, 734, 1133, 1145, 1350, 1433
BfmI CTRYAG 1 cut(s) 598
BfoI RGCGCY 1 cut(s) 156
BfrI CTTAAG 3 cut(s) 671, 847, 1552
BfuI GTATCC 1 cut(s) 505
BglII AGATCT 2 cut(s) 947, 1548
BisI GCNGC 3 cut(s) 531, 561, 598
BlsI GCNGC 3 cut(s) 532, 562, 599
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 2 cut(s) 1458, 1600
BmeT110I CYCGRG 2 cut(s) 400, 1287
BmgT120I GGNCC 3 cut(s) 49, 1458, 1600
BmiI GGNNCC 4 cut(s) 136, 154, 1459, 1476
BmrFI CCNGG 1 cut(s) 747
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 1 cut(s) 961
BmuI ACTGGG 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 2 cut(s) 561, 1461
Bpu14I TTCGAA 1 cut(s) 936
BpuEI CTTGAG 1 cut(s) 1565
BsaAI YACGTR 1 cut(s) 1192
BsaBI GATNNNNATC 2 cut(s) 827, 1377
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 745, 988
BsaWI WCCGGW 1 cut(s) 1125
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 584, 616
Bse1I ACTGG 4 cut(s) 319, 526, 915, 1572
Bse3DI GCAATG 1 cut(s) 568
Bse8I GATNNNNATC 2 cut(s) 827, 1377
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 2 cut(s) 745, 988
BseGI GGATG 3 cut(s) 436, 904, 1522
BseJI GATNNNNATC 2 cut(s) 827, 1377
BseLI CCNNNNNNNGG 2 cut(s) 584, 616
BseMI GCAATG 1 cut(s) 568
BseMII CTCAG 2 cut(s) 437, 449
BseNI ACTGG 4 cut(s) 319, 526, 915, 1572
BseRI GAGGAG 4 cut(s) 170, 175, 294, 871
BseSI GKGCMC 1 cut(s) 1197
BseXI GCAGC 3 cut(s) 517, 572, 584
BshFI GGCC 2 cut(s) 51, 987
BshNI GGYRCC 2 cut(s) 134, 152
BsiHKCI CYCGRG 2 cut(s) 400, 1287
BsiSI CCGG 3 cut(s) 115, 216, 1126
BslFI GGGAC 2 cut(s) 274, 1471
BslI CCNNNNNNNGG 2 cut(s) 584, 616
BsmAI GTCTC 2 cut(s) 692, 853
BsmBI CGTCTC 1 cut(s) 853
BsmFI GGGAC 2 cut(s) 274, 1471
BsnI GGCC 2 cut(s) 51, 987
BsoBI CYCGRG 2 cut(s) 400, 1287
Bsp119I TTCGAA 1 cut(s) 936
Bsp1286I GDGCHC 1 cut(s) 1197
Bsp143I GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
BspACI CCGC 4 cut(s) 63, 102, 723, 1557
BspANI GGCC 2 cut(s) 51, 987
BspCNI CTCAG 2 cut(s) 436, 450
BspHI TCATGA 1 cut(s) 1306
BspLI GGNNCC 4 cut(s) 136, 154, 1459, 1476
BspMAI CTGCAG 1 cut(s) 602
BspPI GGATC 3 cut(s) 467, 1220, 1431
BspT104I TTCGAA 1 cut(s) 936
BspT107I GGYRCC 2 cut(s) 134, 152
BspTI CTTAAG 3 cut(s) 671, 847, 1552
BsrDI GCAATG 1 cut(s) 568
BsrI ACTGG 4 cut(s) 319, 526, 915, 1572
BssECI CCNNGG 2 cut(s) 745, 988
BssMI GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 988
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 4 cut(s) 34, 149, 302, 1337
Bst6I CTCTTC 5 cut(s) 32, 199, 341, 749, 1590
BstACI GRCGYC 1 cut(s) 153
BstAFI CTTAAG 3 cut(s) 671, 847, 1552
BstAPI GCANNNNNTGC 1 cut(s) 606
BstBAI YACGTR 1 cut(s) 1192
BstBI TTCGAA 1 cut(s) 936
BstDEI CTNAG 5 cut(s) 423, 458, 1059, 1443, 1649
BstF5I GGATG 3 cut(s) 436, 904, 1522
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 7 cut(s) 475, 950, 1081, 1228, 1342, 1426, 1551
BstMAI GTCTC 2 cut(s) 692, 853
BstMBI GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
BstMWI GCNNNNNNNGC 2 cut(s) 606, 779
BstNI CCWGG 1 cut(s) 747
BstSCI CCNGG 1 cut(s) 745
BstSFI CTRYAG 1 cut(s) 598
BstSLI GKGCMC 1 cut(s) 1197
BstV1I GCAGC 3 cut(s) 517, 572, 584
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 2 cut(s) 947, 1548
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 2 cut(s) 947, 1548
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 2 cut(s) 51, 987
BtgZI GCGATG 1 cut(s) 763
BtsCI GGATG 3 cut(s) 436, 904, 1522
BtsI GCAGTG 2 cut(s) 607, 792
BtsIMutI CAGTG 3 cut(s) 30, 607, 792
CciI TCATGA 1 cut(s) 1306
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 3 cut(s) 49, 1458, 1600
CseI GACGC 2 cut(s) 92, 522
Csp6I GTAC 3 cut(s) 420, 445, 759
CviAII CATG 6 cut(s) 388, 1238, 1307, 1402, 1453, 1493
CviQI GTAC 3 cut(s) 420, 445, 759
DdeI CTNAG 5 cut(s) 423, 458, 1059, 1443, 1649
DinI GGCGCC 1 cut(s) 154
DpnI GATC 7 cut(s) 474, 949, 1080, 1227, 1341, 1425, 1550
DpnII GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
DraI TTTAAA 1 cut(s) 1090
EaeI YGGCCR 1 cut(s) 985
Eam1104I CTCTTC 5 cut(s) 32, 199, 341, 749, 1590
EarI CTCTTC 5 cut(s) 32, 199, 341, 749, 1590
Eco130I CCWWGG 1 cut(s) 988
Eco47I GGWCC 2 cut(s) 1458, 1600
Eco57I CTGAAG 1 cut(s) 1116
Eco88I CYCGRG 2 cut(s) 400, 1287
EcoO109I RGGNCCY 2 cut(s) 1458, 1600
EcoRI GAATTC 1 cut(s) 1063
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 1 cut(s) 988
EgeI GGCGCC 1 cut(s) 154
EheI GGCGCC 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 988
Esp3I CGTCTC 1 cut(s) 853
FaeI CATG 6 cut(s) 391, 1241, 1310, 1405, 1456, 1496
FalI AAGNNNNNCTT 2 cut(s) 1587, 1619
FaqI GGGAC 2 cut(s) 274, 1471
FatI CATG 6 cut(s) 387, 1237, 1306, 1401, 1452, 1492
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 371
FblI GTMKAC 1 cut(s) 1008
Fnu4HI GCNGC 3 cut(s) 531, 561, 598
FokI GGATG 3 cut(s) 443, 911, 1529
Fsp4HI GCNGC 3 cut(s) 531, 561, 598
FspBI CTAG 7 cut(s) 324, 453, 734, 1133, 1145, 1350, 1433
GlaI GCGC 1 cut(s) 154
GluI GCNGC 3 cut(s) 531, 561, 598
GsuI CTGGAG 2 cut(s) 561, 1461
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 2 cut(s) 51, 987
HapII CCGG 3 cut(s) 115, 216, 1126
HgaI GACGC 2 cut(s) 92, 522
HhaI GCGC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 6 cut(s) 391, 1241, 1310, 1405, 1456, 1496
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 980
HpaII CCGG 3 cut(s) 115, 216, 1126
HphI GGTGA 3 cut(s) 1088, 1112, 1325
Hpy166II GTNNAC 3 cut(s) 259, 316, 1009
Hpy188I TCNGA 5 cut(s) 833, 898, 970, 1069, 1516
Hpy8I GTNNAC 3 cut(s) 259, 316, 1009
Hpy99I CGWCG 2 cut(s) 238, 538
HpyAV CCTTC 3 cut(s) 62, 858, 1313
HpyCH4III ACNGT 4 cut(s) 34, 149, 302, 1337
HpyCH4IV ACGT 4 cut(s) 447, 1103, 1191, 1261
HpyF10VI GCNNNNNNNGC 2 cut(s) 606, 779
HpyF3I CTNAG 5 cut(s) 423, 458, 1059, 1443, 1649
HpySE526I ACGT 4 cut(s) 447, 1103, 1191, 1261
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 6 cut(s) 391, 1241, 1310, 1405, 1456, 1496
HspAI GCGC 1 cut(s) 153
KasI GGCGCC 1 cut(s) 152
Kzo9I GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
LmnI GCTCC 3 cut(s) 379, 1001, 1480
Lsp1109I GCAGC 3 cut(s) 517, 572, 584
LweI GCATC 1 cut(s) 961
MaeI CTAG 7 cut(s) 324, 453, 734, 1133, 1145, 1350, 1433
MaeII ACGT 4 cut(s) 447, 1103, 1191, 1261
MaeIII GTNAC 5 cut(s) 302, 461, 736, 1099, 1262
MalI GATC 7 cut(s) 474, 949, 1080, 1227, 1341, 1425, 1550
MboI GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
MboII GAAGA 9 cut(s) 19, 155, 186, 328, 491, 766, 1322, 1382, 1607
MfeI CAATTG 2 cut(s) 264, 1409
MflI RGATCY 2 cut(s) 947, 1548
MhlI GDGCHC 1 cut(s) 1197
MlsI TGGCCA 1 cut(s) 987
MluNI TGGCCA 1 cut(s) 987
Mly113I GGCGCC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 222
Mox20I TGGCCA 1 cut(s) 987
MscI TGGCCA 1 cut(s) 987
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 987
MspA1I CMGCKG 2 cut(s) 104, 563
MspCI CTTAAG 3 cut(s) 671, 847, 1552
MspI CCGG 3 cut(s) 115, 216, 1126
MspR9I CCNGG 1 cut(s) 747
MunI CAATTG 2 cut(s) 264, 1409
MvaI CCWGG 1 cut(s) 747
MwoI GCNNNNNNNGC 2 cut(s) 606, 779
NarI GGCGCC 1 cut(s) 153
NdeI CATATG 1 cut(s) 371
NdeII GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
NlaIII CATG 6 cut(s) 391, 1241, 1310, 1405, 1456, 1496
NlaIV GGNNCC 4 cut(s) 136, 154, 1459, 1476
NmeAIII GCCGAG 2 cut(s) 702, 1612
NmuCI GTSAC 2 cut(s) 302, 1099
NspV TTCGAA 1 cut(s) 936
OliI CACNNNNGTG 1 cut(s) 309
PaeR7I CTCGAG 2 cut(s) 400, 1287
PagI TCATGA 1 cut(s) 1306
PkrI GCNGC 3 cut(s) 532, 562, 599
PluTI GGCGCC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 1192
PpuMI RGGWCCY 2 cut(s) 1458, 1600
Psp5II RGGWCCY 2 cut(s) 1458, 1600
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 4 cut(s) 136, 154, 1459, 1476
PspPI GGNCC 3 cut(s) 49, 1458, 1600
PspPPI RGGWCCY 2 cut(s) 1458, 1600
PspXI VCTCGAGB 1 cut(s) 400
PstI CTGCAG 1 cut(s) 602
PsuI RGATCY 2 cut(s) 947, 1548
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 3 cut(s) 421, 446, 760
RsaNI GTAC 3 cut(s) 420, 445, 759
RseI CAYNNNNRTG 1 cut(s) 309
SatI GCNGC 3 cut(s) 531, 561, 598
Sau3AI GATC 7 cut(s) 472, 947, 1078, 1225, 1339, 1423, 1548
Sau96I GGNCC 3 cut(s) 49, 1458, 1600
ScrFI CCNGG 1 cut(s) 747
SduI GDGCHC 1 cut(s) 1197
SfaNI GCATC 1 cut(s) 961
SfcI CTRYAG 1 cut(s) 598
SfoI GGCGCC 1 cut(s) 154
Sfr274I CTCGAG 2 cut(s) 400, 1287
SfuI TTCGAA 1 cut(s) 936
SinI GGWCC 2 cut(s) 1458, 1600
SlaI CTCGAG 2 cut(s) 400, 1287
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 7 cut(s) 10, 400, 671, 847, 1287, 1544, 1552
SmoI CTYRAG 7 cut(s) 10, 400, 671, 847, 1287, 1544, 1552
SsiI CCGC 4 cut(s) 63, 102, 723, 1557
SspDI GGCGCC 1 cut(s) 152
SspI AATATT 2 cut(s) 414, 837
SspMI CTAG 7 cut(s) 324, 453, 734, 1133, 1145, 1350, 1433
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 1 cut(s) 988
TaaI ACNGT 4 cut(s) 34, 149, 302, 1337
TaiI ACGT 4 cut(s) 450, 1106, 1194, 1264
TaqI TCGA 6 cut(s) 233, 401, 696, 936, 1288, 1324
TscAI CASTG 3 cut(s) 37, 607, 799
TseFI GTSAC 2 cut(s) 302, 1099
TseI GCWGC 3 cut(s) 530, 560, 597
Tsp45I GTSAC 2 cut(s) 302, 1099
TspDTI ATGAA 8 cut(s) 219, 1207, 1295, 1323, 1383, 1391, 1481, 1608
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 3 cut(s) 37, 607, 799
Vha464I CTTAAG 3 cut(s) 671, 847, 1552
VpaK11BI GGWCC 2 cut(s) 1458, 1600
XapI RAATTY 4 cut(s) 482, 554, 1063, 1085
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XhoI CTCGAG 2 cut(s) 400, 1287
XmiI GTMKAC 1 cut(s) 1008
XspI CTAG 7 cut(s) 324, 453, 734, 1133, 1145, 1350, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.