protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 650 |
| AccBSI | CCGCTC | 1 cut(s) | 393 |
| AccI | GTMKAC | 1 cut(s) | 660 |
| AciI | CCGC | 6 cut(s) | 171, 219, 275, 391, 522, 849 |
| AcoI | YGGCCR | 2 cut(s) | 915, 1266 |
| AcuI | CTGAAG | 1 cut(s) | 706 |
| AfaI | GTAC | 7 cut(s) | 600, 607, 762, 874, 1100, 1121, 1175 |
| AfeI | AGCGCT | 1 cut(s) | 568 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 757 |
| AgsI | TTSAA | 7 cut(s) | 284, 309, 387, 478, 1184, 1220, 1229 |
| AjnI | CCWGG | 3 cut(s) | 465, 571, 1146 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 1278 |
| Alw21I | GWGCWC | 1 cut(s) | 330 |
| Alw26I | GTCTC | 3 cut(s) | 457, 999, 1061 |
| Aor51HI | AGCGCT | 1 cut(s) | 568 |
| AoxI | GGCC | 3 cut(s) | 915, 1141, 1266 |
| ApeKI | GCWGC | 3 cut(s) | 216, 294, 1259 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 638, 670 |
| AspLEI | GCGC | 2 cut(s) | 256, 569 |
| AspS9I | GGNCC | 1 cut(s) | 1271 |
| AsuHPI | GGTGA | 1 cut(s) | 895 |
| AsuNHI | GCTAGC | 1 cut(s) | 173 |
| AvaII | GGWCC | 1 cut(s) | 1271 |
| BalI | TGGCCA | 1 cut(s) | 917 |
| BanI | GGYRCC | 1 cut(s) | 650 |
| BanII | GRGCYC | 1 cut(s) | 330 |
| BbsI | GAAGAC | 1 cut(s) | 738 |
| Bbv12I | GWGCWC | 1 cut(s) | 330 |
| BbvCI | CCTCAGC | 1 cut(s) | 819 |
| BbvI | GCAGC | 3 cut(s) | 228, 281, 1246 |
| BccI | CCATC | 7 cut(s) | 28, 65, 464, 524, 674, 775, 790 |
| BceAI | ACGGC | 5 cut(s) | 202, 273, 949, 955, 1011 |
| BciT130I | CCWGG | 3 cut(s) | 467, 573, 1148 |
| BcoDI | GTCTC | 3 cut(s) | 457, 999, 1061 |
| BfaI | CTAG | 2 cut(s) | 174, 626 |
| BfmI | CTRYAG | 1 cut(s) | 997 |
| BfoI | RGCGCY | 1 cut(s) | 570 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 471 |
| BisI | GCNGC | 4 cut(s) | 217, 295, 522, 1260 |
| BlsI | GCNGC | 4 cut(s) | 218, 296, 523, 1261 |
| Bme1390I | CCNGG | 3 cut(s) | 467, 573, 1148 |
| Bme18I | GGWCC | 1 cut(s) | 1271 |
| BmgT120I | GGNCC | 1 cut(s) | 1271 |
| BmiI | GGNNCC | 2 cut(s) | 535, 652 |
| BmrFI | CCNGG | 3 cut(s) | 467, 573, 1148 |
| BmtI | GCTAGC | 1 cut(s) | 177 |
| BpiI | GAAGAC | 1 cut(s) | 738 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1041, 1073 |
| BpmI | CTGGAG | 2 cut(s) | 555, 1030 |
| Bpu10I | CCTNAGC | 2 cut(s) | 488, 819 |
| Bsa29I | ATCGAT | 1 cut(s) | 23 |
| BsaAI | YACGTR | 1 cut(s) | 132 |
| BsaBI | GATNNNNATC | 1 cut(s) | 19 |
| BsaI | GGTCTC | 1 cut(s) | 1061 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 676, 706, 778, 808, 938, 968 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 757 |
| Bse118I | RCCGGY | 1 cut(s) | 1268 |
| Bse1I | ACTGG | 3 cut(s) | 559, 1096, 1297 |
| Bse3DI | GCAATG | 1 cut(s) | 640 |
| Bse8I | GATNNNNATC | 1 cut(s) | 19 |
| BseBI | CCWGG | 3 cut(s) | 467, 573, 1148 |
| BseCI | ATCGAT | 1 cut(s) | 23 |
| BseGI | GGATG | 3 cut(s) | 60, 475, 663 |
| BseJI | GATNNNNATC | 1 cut(s) | 19 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 757 |
| BseMI | GCAATG | 1 cut(s) | 640 |
| BseMII | CTCAG | 5 cut(s) | 468, 514, 812, 833, 1238 |
| BseNI | ACTGG | 3 cut(s) | 559, 1096, 1297 |
| BseRI | GAGGAG | 4 cut(s) | 151, 786, 807, 1017 |
| BseX3I | CGGCCG | 1 cut(s) | 1266 |
| BseXI | GCAGC | 3 cut(s) | 228, 281, 1246 |
| BseYI | CCCAGC | 2 cut(s) | 621, 710 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1269 |
| BshFI | GGCC | 3 cut(s) | 917, 1143, 1268 |
| BshNI | GGYRCC | 1 cut(s) | 650 |
| BshVI | ATCGAT | 1 cut(s) | 23 |
| BsiEI | CGRYCG | 1 cut(s) | 1269 |
| BsiHKAI | GWGCWC | 1 cut(s) | 330 |
| BsiSI | CCGG | 2 cut(s) | 611, 1269 |
| BslFI | GGGAC | 3 cut(s) | 318, 604, 1056 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 757 |
| BsmAI | GTCTC | 3 cut(s) | 457, 999, 1061 |
| BsmBI | CGTCTC | 1 cut(s) | 457 |
| BsmFI | GGGAC | 3 cut(s) | 318, 604, 1056 |
| BsmI | GAATGC | 2 cut(s) | 98, 290 |
| BsnI | GGCC | 3 cut(s) | 917, 1143, 1268 |
| Bso31I | GGTCTC | 1 cut(s) | 1061 |
| Bsp1286I | GDGCHC | 1 cut(s) | 330 |
| Bsp1407I | TGTACA | 2 cut(s) | 598, 1173 |
| Bsp143I | GATC | 2 cut(s) | 235, 1130 |
| BspACI | CCGC | 6 cut(s) | 171, 219, 275, 391, 522, 849 |
| BspANI | GGCC | 3 cut(s) | 917, 1143, 1268 |
| BspCNI | CTCAG | 5 cut(s) | 467, 513, 811, 832, 1239 |
| BspDI | ATCGAT | 1 cut(s) | 23 |
| BspHI | TCATGA | 1 cut(s) | 1279 |
| BspLI | GGNNCC | 2 cut(s) | 535, 652 |
| BspOI | GCTAGC | 1 cut(s) | 177 |
| BspT107I | GGYRCC | 1 cut(s) | 650 |
| BspTNI | GGTCTC | 1 cut(s) | 1061 |
| BsrBI | CCGCTC | 1 cut(s) | 393 |
| BsrDI | GCAATG | 1 cut(s) | 640 |
| BsrFI | RCCGGY | 1 cut(s) | 1268 |
| BsrGI | TGTACA | 2 cut(s) | 598, 1173 |
| BsrI | ACTGG | 3 cut(s) | 559, 1096, 1297 |
| BssAI | RCCGGY | 1 cut(s) | 1268 |
| BssMI | GATC | 2 cut(s) | 235, 1130 |
| Bst2UI | CCWGG | 3 cut(s) | 467, 573, 1148 |
| Bst4CI | ACNGT | 7 cut(s) | 182, 368, 603, 650, 872, 998, 1119 |
| Bst6I | CTCTTC | 2 cut(s) | 171, 1281 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 639 |
| BstAUI | TGTACA | 2 cut(s) | 598, 1173 |
| BstBAI | YACGTR | 1 cut(s) | 132 |
| BstC8I | GCNNGC | 3 cut(s) | 175, 565, 853 |
| BstDEI | CTNAG | 8 cut(s) | 409, 454, 488, 500, 643, 798, 819, 1247 |
| BstF5I | GGATG | 3 cut(s) | 60, 475, 663 |
| BstH2I | RGCGCY | 1 cut(s) | 570 |
| BstHHI | GCGC | 2 cut(s) | 256, 569 |
| BstKTI | GATC | 2 cut(s) | 238, 1133 |
| BstMAI | GTCTC | 3 cut(s) | 457, 999, 1061 |
| BstMBI | GATC | 2 cut(s) | 235, 1130 |
| BstMCI | CGRYCG | 1 cut(s) | 1269 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 225, 294, 471, 639, 778, 1032, 1265 |
| BstNI | CCWGG | 3 cut(s) | 467, 573, 1148 |
| BstNSI | RCATGY | 1 cut(s) | 1114 |
| BstSCI | CCNGG | 3 cut(s) | 465, 571, 1146 |
| BstSFI | CTRYAG | 1 cut(s) | 997 |
| BstV1I | GCAGC | 3 cut(s) | 228, 281, 1246 |
| BstV2I | GAAGAC | 1 cut(s) | 738 |
| BstZI | CGGCCG | 1 cut(s) | 1266 |
| Bsu15I | ATCGAT | 1 cut(s) | 23 |
| BsuRI | GGCC | 3 cut(s) | 917, 1143, 1268 |
| BsuTUI | ATCGAT | 1 cut(s) | 23 |
| BtgZI | GCGATG | 3 cut(s) | 282, 753, 994 |
| BtsCI | GGATG | 3 cut(s) | 60, 475, 663 |
| BtsI | GCAGTG | 1 cut(s) | 776 |
| BtsIMutI | CAGTG | 3 cut(s) | 608, 776, 994 |
| Cac8I | GCNNGC | 3 cut(s) | 175, 565, 853 |
| CciI | TCATGA | 1 cut(s) | 1279 |
| CfoI | GCGC | 2 cut(s) | 256, 569 |
| Cfr10I | RCCGGY | 1 cut(s) | 1268 |
| Cfr13I | GGNCC | 1 cut(s) | 1271 |
| ClaI | ATCGAT | 1 cut(s) | 23 |
| CseI | GACGC | 1 cut(s) | 447 |
| Csp6I | GTAC | 7 cut(s) | 599, 606, 761, 873, 1099, 1120, 1174 |
| CviAII | CATG | 3 cut(s) | 245, 1111, 1280 |
| CviQI | GTAC | 7 cut(s) | 599, 606, 761, 873, 1099, 1120, 1174 |
| DdeI | CTNAG | 8 cut(s) | 409, 454, 488, 500, 643, 798, 819, 1247 |
| DpnI | GATC | 2 cut(s) | 237, 1132 |
| DpnII | GATC | 2 cut(s) | 235, 1130 |
| EaeI | YGGCCR | 2 cut(s) | 915, 1266 |
| EagI | CGGCCG | 1 cut(s) | 1266 |
| Eam1104I | CTCTTC | 2 cut(s) | 171, 1281 |
| EarI | CTCTTC | 2 cut(s) | 171, 1281 |
| Ecl136II | GAGCTC | 1 cut(s) | 328 |
| EclXI | CGGCCG | 1 cut(s) | 1266 |
| Eco24I | GRGCYC | 1 cut(s) | 330 |
| Eco31I | GGTCTC | 1 cut(s) | 1061 |
| Eco47I | GGWCC | 1 cut(s) | 1271 |
| Eco47III | AGCGCT | 1 cut(s) | 568 |
| Eco52I | CGGCCG | 1 cut(s) | 1266 |
| Eco53kI | GAGCTC | 1 cut(s) | 328 |
| Eco57I | CTGAAG | 1 cut(s) | 706 |
| EcoICRI | GAGCTC | 1 cut(s) | 328 |
| EcoRII | CCWGG | 3 cut(s) | 465, 571, 1146 |
| EcoT38I | GRGCYC | 1 cut(s) | 330 |
| Esp3I | CGTCTC | 1 cut(s) | 457 |
| FaeI | CATG | 3 cut(s) | 248, 1114, 1283 |
| FaqI | GGGAC | 3 cut(s) | 318, 604, 1056 |
| FatI | CATG | 3 cut(s) | 244, 1110, 1279 |
| FauI | CCCGC | 3 cut(s) | 178, 212, 856 |
| FblI | GTMKAC | 1 cut(s) | 660 |
| Fnu4HI | GCNGC | 4 cut(s) | 217, 295, 522, 1260 |
| FokI | GGATG | 3 cut(s) | 47, 482, 650 |
| FriOI | GRGCYC | 1 cut(s) | 330 |
| Fsp4HI | GCNGC | 4 cut(s) | 217, 295, 522, 1260 |
| FspBI | CTAG | 2 cut(s) | 174, 626 |
| GlaI | GCGC | 2 cut(s) | 255, 568 |
| GluI | GCNGC | 4 cut(s) | 217, 295, 522, 1260 |
| GsaI | CCCAGC | 2 cut(s) | 625, 714 |
| GsuI | CTGGAG | 2 cut(s) | 555, 1030 |
| HaeII | RGCGCY | 1 cut(s) | 570 |
| HaeIII | GGCC | 3 cut(s) | 917, 1143, 1268 |
| HapII | CCGG | 2 cut(s) | 611, 1269 |
| HgaI | GACGC | 1 cut(s) | 447 |
| HhaI | GCGC | 2 cut(s) | 256, 569 |
| Hin1II | CATG | 3 cut(s) | 248, 1114, 1283 |
| Hin6I | GCGC | 2 cut(s) | 254, 567 |
| HinP1I | GCGC | 2 cut(s) | 254, 567 |
| HindIII | AAGCTT | 1 cut(s) | 1209 |
| HinfI | GANTC | 5 cut(s) | 305, 405, 504, 699, 1043 |
| HpaII | CCGG | 2 cut(s) | 611, 1269 |
| HphI | GGTGA | 1 cut(s) | 895 |
| Hpy166II | GTNNAC | 3 cut(s) | 439, 606, 661 |
| Hpy188I | TCNGA | 4 cut(s) | 364, 503, 725, 992 |
| Hpy188III | TCNNGA | 6 cut(s) | 12, 52, 1047, 1184, 1202, 1280 |
| Hpy8I | GTNNAC | 3 cut(s) | 439, 606, 661 |
| HpyAV | CCTTC | 2 cut(s) | 920, 1075 |
| HpyCH4III | ACNGT | 7 cut(s) | 182, 368, 603, 650, 872, 998, 1119 |
| HpyCH4IV | ACGT | 4 cut(s) | 131, 450, 756, 1188 |
| HpyCH4V | TGCA | 6 cut(s) | 261, 551, 593, 633, 986, 1110 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 225, 294, 471, 639, 778, 1032, 1265 |
| HpyF3I | CTNAG | 8 cut(s) | 409, 454, 488, 500, 643, 798, 819, 1247 |
| HpySE526I | ACGT | 4 cut(s) | 131, 450, 756, 1188 |
| Hsp92II | CATG | 3 cut(s) | 248, 1114, 1283 |
| HspAI | GCGC | 2 cut(s) | 254, 567 |
| Kzo9I | GATC | 2 cut(s) | 235, 1130 |
| LmnI | GCTCC | 2 cut(s) | 398, 574 |
| Lsp1109I | GCAGC | 3 cut(s) | 228, 281, 1246 |
| MaeI | CTAG | 2 cut(s) | 174, 626 |
| MaeII | ACGT | 4 cut(s) | 131, 450, 756, 1188 |
| MaeIII | GTNAC | 7 cut(s) | 45, 182, 577, 716, 884, 929, 1113 |
| MalI | GATC | 2 cut(s) | 237, 1132 |
| MbiI | CCGCTC | 1 cut(s) | 393 |
| MboI | GATC | 2 cut(s) | 235, 1130 |
| MboII | GAAGA | 5 cut(s) | 149, 158, 198, 743, 1268 |
| MfeI | CAATTG | 1 cut(s) | 1105 |
| MhlI | GDGCHC | 1 cut(s) | 330 |
| MlsI | TGGCCA | 1 cut(s) | 917 |
| MluCI | AATT | 6 cut(s) | 112, 202, 249, 1105, 1152, 1162 |
| MluNI | TGGCCA | 1 cut(s) | 917 |
| MlyI | GAGTC | 2 cut(s) | 708, 1037 |
| MmeI | TCCRAC | 1 cut(s) | 970 |
| Mox20I | TGGCCA | 1 cut(s) | 917 |
| MscI | TGGCCA | 1 cut(s) | 917 |
| MseI | TTAA | 3 cut(s) | 447, 543, 1038 |
| Msp20I | TGGCCA | 1 cut(s) | 917 |
| MspA1I | CMGCKG | 3 cut(s) | 219, 277, 714 |
| MspI | CCGG | 2 cut(s) | 611, 1269 |
| MspR9I | CCNGG | 3 cut(s) | 467, 573, 1148 |
| MunI | CAATTG | 1 cut(s) | 1105 |
| Mva1269I | GAATGC | 2 cut(s) | 98, 290 |
| MvaI | CCWGG | 3 cut(s) | 467, 573, 1148 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 225, 294, 471, 639, 778, 1032, 1265 |
| NdeII | GATC | 2 cut(s) | 235, 1130 |
| NheI | GCTAGC | 1 cut(s) | 173 |
| NlaIII | CATG | 3 cut(s) | 248, 1114, 1283 |
| NlaIV | GGNNCC | 2 cut(s) | 535, 652 |
| NmuCI | GTSAC | 1 cut(s) | 577 |
| NspI | RCATGY | 1 cut(s) | 1114 |
| PagI | TCATGA | 1 cut(s) | 1279 |
| PctI | GAATGC | 2 cut(s) | 98, 290 |
| PfeI | GAWTC | 3 cut(s) | 305, 405, 504 |
| PfoI | TCCNGGA | 1 cut(s) | 1146 |
| PkrI | GCNGC | 4 cut(s) | 218, 296, 523, 1261 |
| PleI | GAGTC | 2 cut(s) | 707, 1037 |
| PpsI | GAGTC | 2 cut(s) | 707, 1037 |
| Ppu21I | YACGTR | 1 cut(s) | 132 |
| Psp124BI | GAGCTC | 1 cut(s) | 330 |
| Psp6I | CCWGG | 3 cut(s) | 465, 571, 1146 |
| PspFI | CCCAGC | 2 cut(s) | 621, 710 |
| PspGI | CCWGG | 3 cut(s) | 465, 571, 1146 |
| PspN4I | GGNNCC | 2 cut(s) | 535, 652 |
| PspPI | GGNCC | 1 cut(s) | 1271 |
| PvuII | CAGCTG | 1 cut(s) | 714 |
| RsaI | GTAC | 7 cut(s) | 600, 607, 762, 874, 1100, 1121, 1175 |
| RsaNI | GTAC | 7 cut(s) | 599, 606, 761, 873, 1099, 1120, 1174 |
| SacI | GAGCTC | 1 cut(s) | 330 |
| SaqAI | TTAA | 3 cut(s) | 447, 543, 1038 |
| SatI | GCNGC | 4 cut(s) | 217, 295, 522, 1260 |
| Sau3AI | GATC | 2 cut(s) | 235, 1130 |
| Sau96I | GGNCC | 1 cut(s) | 1271 |
| SchI | GAGTC | 2 cut(s) | 708, 1037 |
| ScrFI | CCNGG | 3 cut(s) | 467, 573, 1148 |
| SduI | GDGCHC | 1 cut(s) | 330 |
| SfcI | CTRYAG | 1 cut(s) | 997 |
| SinI | GGWCC | 1 cut(s) | 1271 |
| SmlI | CTYRAG | 1 cut(s) | 10 |
| SmoI | CTYRAG | 1 cut(s) | 10 |
| Sse9I | AATT | 6 cut(s) | 112, 202, 249, 1105, 1152, 1162 |
| SsiI | CCGC | 6 cut(s) | 171, 219, 275, 391, 522, 849 |
| SspMI | CTAG | 2 cut(s) | 174, 626 |
| SstI | GAGCTC | 1 cut(s) | 330 |
| StyD4I | CCNGG | 3 cut(s) | 465, 571, 1146 |
| TaaI | ACNGT | 7 cut(s) | 182, 368, 603, 650, 872, 998, 1119 |
| TaiI | ACGT | 4 cut(s) | 134, 453, 759, 1191 |
| TaqI | TCGA | 3 cut(s) | 23, 976, 1203 |
| TasI | AATT | 6 cut(s) | 112, 202, 249, 1105, 1152, 1162 |
| TatI | WGTACW | 2 cut(s) | 598, 1173 |
| TauI | GCSGC | 1 cut(s) | 524 |
| TfiI | GAWTC | 3 cut(s) | 305, 405, 504 |
| Tru1I | TTAA | 3 cut(s) | 447, 543, 1038 |
| Tru9I | TTAA | 3 cut(s) | 447, 543, 1038 |
| TscAI | CASTG | 3 cut(s) | 608, 783, 1001 |
| TseFI | GTSAC | 1 cut(s) | 577 |
| TseI | GCWGC | 3 cut(s) | 216, 294, 1259 |
| Tsp45I | GTSAC | 1 cut(s) | 577 |
| TspDTI | ATGAA | 6 cut(s) | 105, 150, 233, 744, 1268, 1299 |
| TspGWI | ACGGA | 1 cut(s) | 728 |
| TspRI | CASTG | 3 cut(s) | 608, 783, 1001 |
| VpaK11BI | GGWCC | 1 cut(s) | 1271 |
| XceI | RCATGY | 1 cut(s) | 1114 |
| XmiI | GTMKAC | 1 cut(s) | 660 |
| XspI | CTAG | 2 cut(s) | 174, 626 |