Rw5G003500

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000086
Physical Location & Seq
Forward (+)
3283102 .. 3286854
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003500.1

Sequence Viewer

Length: 1689 bp
ATGAGTTCCTCAAGATTGCTTTTCTTCATGCTAATTCCTATTATCTTGGACCTTGTTGCTCCTGCCACAGCGCAAATTGATGACGGTGTATGTACTTTCACAGCTGATTTCTGTTGGAGATGTTCCAACATGGGCACCTACACTGATGGCAGCTTCTACCAAGAAAACCTCAACAACCTCCTGTCCAACTTCTCTTCCAACACCCAAATCAATTACGGGTTTTACAATTCTTCCATGGGACAGGAAGGAGATATCGCACTGGACATGTGCCATACTTGTGTCAAGGAGGCCAGTCAACTTCTCTTCCAGAATTGCTCACATCAAAAGGAAGCGATCCTATGGGCTCAGCCTTGTATGGTACGATACTCGAGCAAGTTAATATTTGGTATTCAGGAAGATGAACCTATCAAGTATGTCCCTAGCCCCACCGATGCAGCGAACATAGAACAGTTTACACCACTGTTAAATTCCTTTTTGGATACCTTAAGTGAGAAACCGGCATCAGGGGACTCTCTCAAAAAATATTCAGCAGGACATACAACTCTCCAAGTTGTTCCAAAATATCAAGCAATAGATGGATTTGTTCAGTGCACTCCAGATATGGACAAGAACAATTGCAGCAATTGCCTACAAGATGCTATCTCAAAAATTCCAACATGTTGTGGTGGAAAGCAAGGAGGCAGAGTTCTTAAACCCAGCTGCAGTGCAAGAAAGAAGGGGAACGCGTCAAAAACTACCATGATCATCATCATTGTGGCCGTGATTGCTTTTATCCTCACACTTCTCGTCAGCATTTGCATTTTCTTTAGAGCAAAGAATCAGAATCTTGAAAATGATAATTCCGGTGAAATTAGTTTGGCAGATTCATTGCAATATGACTTCGAAACTATTAGAGATGCAACAGATGACTTCTCTGATGAAAATAAGCTTGGACAAGGTGGATTTGGAGCTGCTTATAAGGGTGACCAAGAGTTCAAAAATGAGGTCACATTAGTTGCTCAACTTCAACATCGGAATTTAGTAAGGCTCCTGGGATTTTATCCAATCAATCACGGGCATTTGGATTGGGACACACGTTACAAAATCATAGGAGATTCTCGACTTCGAATTATCCATCGTGACCTTAAACCTAGCAATGTTTTATTAGATGGGGATATGAATCCTAAAATTGCAGATTTTGGCATGGCAAGATTATTTCTGATGGATCAAACTCAAGGACACACAAACAGAGTCGTAGGGACCTATGGATGCATGGCTCCAGAGTATGCCATCCATGGGCGTTTCACTGTCAAATCGGATGTCTTCAGTTTTGGTGTGTTAGTTCTTGAGATTGTGAGTGGCAAGAAAGTGAGCACTTTTCGGAATGGTGAGAATGAAGAGGACCTTCTCAGCTATGCATGGCGAAGTTGGAAAGAGGACACAGTTCATAATATCATAGATCCAATGTTGACAAAGAGTTCAAGGATTGAAACAATGAGATGTATTCACATTGGCTTACTCTGCGTCCAAGAAAATTTGAATAGTAGACCAACCATGTCTTCAGTTGTTTCCATGCTGAATAGCGATTCTGTTACACTCTCCCTACCATTGCGACCTAAATATTTTTGGCAATATAATGATGAATCAGACATCAACGAACTGACAGAGTCAAGAAATGAGGCTTCAATTACGAATCCATATCCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

562

Amino Acids

62.97

Weight (kDa)

5.47

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 41 - 124 1e-12 Salt stress response/antifungal
Stress-antifung PF01657 148 - 237 8.1e-09 Salt stress response/antifungal
Pkinase PF00069 369 - 516 1.5e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 369 - 519 5e-20 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21230 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21410 AT4G21410 AT4G21410 AT4G38830
fragaria_vesca FvH4_2g29861 FvH4_2g29861 FvH4_2g29870 FvH4_2g29870 FvH4_2g29870 FvH4_2g29871 FvH4_2g29871 FvH4_2g29875 FvH4_2g29878 FvH4_3g02833 FvH4_3g02833 FvH4_3g02834 FvH4_3g02870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g20010
malus_domestica MD00G1101400.v1.1 MD00G1101700.v1.1 MD01G1027900.v1.1 MD02G1101400.v1.1 MD05G1336800.v1.1 MD05G1337000.v1.1 MD05G1337300.v1.1 MD05G1337400.v1.1 MD05G1337500.v1.1 MD07G1014000.v1.1 MD08G1139800.v1.1 MD08G1139900.v1.1 MD08G1140000.v1.1 MD08G1140100.v1.1 MD08G1140200.v1.1 MD08G1140400.v1.1 MD10G1312200.v1.1 MD10G1312500.v1.1 MD15G1117400.v1.1
prunus_persica Prupe.1G174500_v2.0.a1 Prupe.1G468600_v2.0.a1 Prupe.1G469000_v2.0.a1 Prupe.1G469100_v2.0.a1 Prupe.1G469200_v2.0.a1 Prupe.1G469300_v2.0.a1 Prupe.4G027900_v2.0.a1 Prupe.4G028000_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028200_v2.0.a1 Prupe.4G028600_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028800_v2.0.a1 Prupe.4G100700_v2.0.a1 Prupe.6G183400_v2.0.a1 Prupe.6G183500_v2.0.a1 Prupe.6G183600_v2.0.a1 Prupe.6G184100_v2.0.a1 Prupe.6G184400_v2.0.a1 Prupe.6G184500_v2.0.a1
pyrus_communis pycom01g05620 pycom01g05630 pycom05g30820 pycom05g30840 pycom05g30860 pycom05g30880 pycom05g30890 pycom05g30900 pycom08g11780 pycom08g11850 pycom10g26340 pycom10g26350 pycom10g26360 pycom10g26400 pycom10g26410 pycom15g10580
rosa_chinensis RchiOBHm_Chr1g0313011 RchiOBHm_Chr1g0319461 RchiOBHm_Chr1g0319511 RchiOBHm_Chr2g0114611 RchiOBHm_Chr2g0114631 RchiOBHm_Chr2g0114691 RchiOBHm_Chr2g0114741 RchiOBHm_Chr2g0114751 RchiOBHm_Chr2g0114781 RchiOBHm_Chr2g0114791 RchiOBHm_Chr3g0494511 RchiOBHm_Chr3g0494521 RchiOBHm_Chr4g0413731 RchiOBHm_Chr5g0004271 RchiOBHm_Chr5g0004291 RchiOBHm_Chr5g0004301 RchiOBHm_Chr5g0004341 RchiOBHm_Chr5g0004351 RchiOBHm_Chr5g0004371 RchiOBHm_Chr5g0033301 RchiOBHm_Chr5g0033311 RchiOBHm_Chr5g0033341 RchiOBHm_Chr5g0033361 RchiOBHm_Chr5g0033391 RchiOBHm_Chr5g0033401 RchiOBHm_Chr5g0033431 RchiOBHm_Chr5g0033451 RchiOBHm_Chr5g0033461 RchiOBHm_Chr5g0033531 RchiOBHm_Chr5g0033541 RchiOBHm_Chr5g0033711 RchiOBHm_Chr5g0033851 RchiOBHm_Chr5g0033861 RchiOBHm_Chr5g0033871 RchiOBHm_Chr5g0054051 RchiOBHm_Chr5g0069631 RchiOBHm_Chr6g0265231 RchiOBHm_Chr6g0298941 RchiOBHm_Chr6g0298951 RchiOBHm_Chr6g0299001 RchiOBHm_Chr6g0299011 RchiOBHm_Chr6g0299021
rosa_laevigata RLG00000008198 RLG00000010335 RLG00000011468 RLG00000011471 RLG00000014070 RLG00000014071 RLG00000018116 RLG00000018125 RLG00000031219 RLG00000031220 RLG00000031221 RLG00000031222 RLG00000031224 RLG00000033469 RLG00000033471 RLG00000033473 RLG00000033476 RLG00000033477 RLG00000033479 RLG00000033480 RLG00000033483 RLG00000035638
rosa_multiflora Rmu_co8063382.1_g000001 Rmu_co8095246.1_g000001 Rmu_sc0000370.1_g000009 Rmu_sc0000370.1_g000019 Rmu_sc0000371.1_g000023 Rmu_sc0000371.1_g000036 Rmu_sc0000433.1_g000023 Rmu_sc0000433.1_g000024 Rmu_sc0000545.1_g000004 Rmu_sc0000981.1_g000001 Rmu_sc0001150.1_g000032 Rmu_sc0001242.1_g000004 Rmu_sc0001242.1_g000008 Rmu_sc0001522.1_g000035 Rmu_sc0002206.1_g000008 Rmu_sc0002206.1_g000021 Rmu_sc0002453.1_g000008 Rmu_sc0002453.1_g000018 Rmu_sc0003286.1_g000007 Rmu_sc0003286.1_g000013 Rmu_sc0004133.1_g000005 Rmu_sc0005660.1_g000005 Rmu_sc0005660.1_g000019 Rmu_sc0005660.1_g000025 Rmu_sc0005870.1_g000017 Rmu_sc0005870.1_g000022 Rmu_sc0006083.1_g000003 Rmu_sc0008092.1_g000016 Rmu_sc0008210.1_g000007 Rmu_sc0009715.1_g000001 Rmu_sc0011893.1_g000003 Rmu_sc0011893.1_g000004 Rmu_sc0015325.1_g000002 Rmu_sc0029922.1_g000001 Rmu_ssc0000359.1_g000017 Rmu_ssc0000424.1_g000009
rosa_roxburghii Rroxscaffold_175G00432170 Rroxscaffold_1G00022110 Rroxscaffold_1G00046520 Rroxscaffold_1G00046550 Rroxscaffold_1G00046600 Rroxscaffold_1G00046650 Rroxscaffold_1G00046680 Rroxscaffold_1G00071130 Rroxscaffold_1G00071380 Rroxscaffold_1G00071410 Rroxscaffold_2G00130310 Rroxscaffold_2G00130320 Rroxscaffold_2G00130330 Rroxscaffold_4G00328340 Rroxscaffold_4G00328360 Rroxscaffold_4G00328370 Rroxscaffold_5G00357650 Rroxscaffold_7G00156890 Rroxscaffold_7G00169080 Rroxscaffold_7G00169090 Rroxscaffold_7G00169100 Rroxscaffold_7G00169170 Rroxscaffold_7G00169190
rosa_rugosa Rorug01G0027900 Rorug01G0130600 Rorug02G0190700 Rorug04G0411000 Rorug04G0411000 Rorug04G0411200 Rorug04G0411300 Rorug04G0411400 Rorug04G0411500 Rorug05G0139300 Rorug05G0139500 Rorug05G0139800 Rorug05G0139900 Rorug05G0187000 Rorug05G0187000 Rorug05G0187000 Rorug05G0398900 Rorug06G0034600 Rorug06G0283900 Rorug06G0284000 Rorug06G0284100 Rorug06G0394700
rosa_samantha Rh1BG036800 Rh1CG040700 Rh1CG040800 Rh1DG000200 Rh1DG028900 Rh1DG090600 Rh2AG248900 Rh2CG252600 Rh2CG253100 Rh2CG253500 Rh2CG253600 Rh2CG253900 Rh4DG177700 Rh5CG041500 Rh5CG041700 Rh5CG042100 Rh5CG260400 Rh5CG260500 Rh5CG260600 Rh5CG260900 Rh5CG261100 Rh5DG037300 Rh5DG037400 Rh5DG037500 Rh5DG236800 Rh5DG236900 Rh5DG237100 Rh5DG237500 Rh5DG237600 Rh5DG237700 Rh5DG241100 Rh6CG134500 Rh6CG409700 Rh6CG409800 Rh6CG410100 Rh6DG120900 Rh6DG396500 Rh6DG396600 Rh6DG396700 Rh6DG514400
rosa_wichuraiana Rw1G003200 Rw1G003210 Rw1G003230 Rw2G019350 Rw4G015240 Rw5G003500 Rw5G003520 Rw5G003530 Rw5G003690 Rw5G003720 Rw5G003730 Rw5G021090 Rw5G021100 Rw5G021110 Rw5G021120 Rw5G021130 Rw6G011870 Rw6G034630 Rw7G038700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 957
AccB1I GGYRCC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 1525
AccII CGCG 1 cut(s) 725
AclWI GGATC 3 cut(s) 328, 1212, 1433
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 4 cut(s) 466, 648, 1015, 1513
AcuI CTGAAG 2 cut(s) 1288, 1524
AfaI GTAC 2 cut(s) 94, 360
AfiI CCNNNNNNNGG 2 cut(s) 503, 1275
AflII CTTAAG 1 cut(s) 484
AflIII ACRYGT 4 cut(s) 264, 656, 723, 1073
AgsI TTSAA 7 cut(s) 830, 976, 1007, 1461, 1469, 1519, 1665
AjnI CCWGG 1 cut(s) 1029
AjuI GAANNNNNNNTTGG 4 cut(s) 912, 944, 1522, 1554
AloI GAACNNNNNNTCC 2 cut(s) 669, 701
AluBI AGCT 6 cut(s) 104, 153, 699, 928, 950, 1392
AluI AGCT 6 cut(s) 104, 153, 699, 928, 950, 1392
Alw21I GWGCWC 2 cut(s) 593, 1355
Alw44I GTGCAC 1 cut(s) 589
AlwI GGATC 3 cut(s) 328, 1212, 1433
Ama87I CYCGRG 1 cut(s) 367
AoxI GGCC 2 cut(s) 288, 756
ApaLI GTGCAC 1 cut(s) 589
ApeKI GCWGC 5 cut(s) 150, 434, 618, 699, 950
ApoI RAATTY 4 cut(s) 466, 648, 1015, 1513
ArsI GACNNNNNNTTYG 4 cut(s) 969, 1001, 1284, 1316
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 3 cut(s) 49, 1239, 1381
AsuHPI GGTGA 3 cut(s) 857, 974, 1379
AsuII TTCGAA 2 cut(s) 882, 1105
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 3 cut(s) 49, 1239, 1381
BaeGI GKGCMC 2 cut(s) 137, 593
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 1294, 1530
Bbv12I GWGCWC 2 cut(s) 593, 1355
BbvI GCAGC 5 cut(s) 162, 446, 630, 686, 937
BccI CCATC 6 cut(s) 140, 569, 1122, 1142, 1195, 1277
BceAI ACGGC 1 cut(s) 743
BciT130I CCWGG 1 cut(s) 1031
BciVI GTATCC 1 cut(s) 472
BclI TGATCA 1 cut(s) 741
BfaI CTAG 3 cut(s) 420, 1131, 1687
BfmI CTRYAG 1 cut(s) 700
BfrI CTTAAG 1 cut(s) 484
BfuI GTATCC 1 cut(s) 472
BisI GCNGC 5 cut(s) 151, 435, 619, 700, 951
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 5 cut(s) 152, 436, 620, 701, 952
Bme1390I CCNGG 1 cut(s) 1031
Bme18I GGWCC 3 cut(s) 49, 1239, 1381
BmeT110I CYCGRG 1 cut(s) 367
BmgT120I GGNCC 3 cut(s) 49, 1239, 1381
BmiI GGNNCC 4 cut(s) 136, 1028, 1240, 1257
BmrFI CCNGG 1 cut(s) 1031
BmsI GCATC 5 cut(s) 421, 509, 625, 886, 1238
BpiI GAAGAC 2 cut(s) 1294, 1530
BpmI CTGGAG 2 cut(s) 579, 1242
Bpu1102I GCTNAGC 1 cut(s) 345
Bpu14I TTCGAA 2 cut(s) 882, 1105
BpuEI CTTGAG 2 cut(s) 1197, 1346
BsaBI GATNNNNATC 2 cut(s) 746, 1158
BsaJI CCNNGG 3 cut(s) 234, 1030, 1273
BsaWI WCCGGW 1 cut(s) 842
BsaXI ACNNNNNCTCC 2 cut(s) 669, 699
Bsc4I CCNNNNNNNGG 2 cut(s) 503, 1275
Bse118I RCCGGY 1 cut(s) 496
Bse1I ACTGG 2 cut(s) 264, 291
Bse3DI GCAATG 3 cut(s) 866, 1141, 1586
Bse8I GATNNNNATC 2 cut(s) 746, 1158
BseBI CCWGG 1 cut(s) 1031
BseDI CCNNGG 3 cut(s) 234, 1030, 1273
BseGI GGATG 3 cut(s) 1253, 1269, 1303
BseJI GATNNNNATC 2 cut(s) 746, 1158
BseLI CCNNNNNNNGG 2 cut(s) 503, 1275
BseMI GCAATG 3 cut(s) 866, 1141, 1586
BseMII CTCAG 2 cut(s) 359, 1402
BseNI ACTGG 2 cut(s) 264, 291
BseSI GKGCMC 2 cut(s) 137, 593
BseXI GCAGC 5 cut(s) 162, 446, 630, 686, 937
BseYI CCCAGC 1 cut(s) 695
Bsh1236I CGCG 1 cut(s) 725
BshFI GGCC 2 cut(s) 290, 758
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 593, 1355
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 2 cut(s) 497, 843
BslFI GGGAC 5 cut(s) 252, 401, 521, 1082, 1252
BslI CCNNNNNNNGG 2 cut(s) 503, 1275
BsmFI GGGAC 5 cut(s) 252, 401, 521, 1082, 1252
BsnI GGCC 2 cut(s) 290, 758
BsoBI CYCGRG 1 cut(s) 367
Bsp119I TTCGAA 2 cut(s) 882, 1105
Bsp1286I GDGCHC 4 cut(s) 137, 346, 593, 1355
Bsp143I GATC 4 cut(s) 333, 741, 1204, 1438
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp19I CCATGG 2 cut(s) 234, 1273
BspANI GGCC 2 cut(s) 290, 758
BspCNI CTCAG 2 cut(s) 358, 1401
BspFNI CGCG 1 cut(s) 725
BspLI GGNNCC 4 cut(s) 136, 1028, 1240, 1257
BspMAI CTGCAG 1 cut(s) 704
BspPI GGATC 3 cut(s) 328, 1212, 1433
BspT104I TTCGAA 2 cut(s) 882, 1105
BspT107I GGYRCC 1 cut(s) 134
BspTI CTTAAG 1 cut(s) 484
BsrDI GCAATG 3 cut(s) 866, 1141, 1586
BsrFI RCCGGY 1 cut(s) 496
BsrI ACTGG 2 cut(s) 264, 291
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 3 cut(s) 234, 1030, 1273
BssMI GATC 4 cut(s) 333, 741, 1204, 1438
BssT1I CCWWGG 2 cut(s) 234, 1273
Bst2UI CCWGG 1 cut(s) 1031
Bst4CI ACNGT 5 cut(s) 86, 450, 462, 1288, 1423
Bst6I CTCTTC 3 cut(s) 199, 308, 1371
BstAFI CTTAAG 1 cut(s) 484
BstAPI GCANNNNNTGC 1 cut(s) 624
BstBI TTCGAA 2 cut(s) 882, 1105
BstDEI CTNAG 2 cut(s) 345, 1388
BstDSI CCRYGG 2 cut(s) 234, 1273
BstEII GGTNACC 1 cut(s) 962
BstF5I GGATG 3 cut(s) 1253, 1269, 1303
BstFNI CGCG 1 cut(s) 725
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 4 cut(s) 336, 744, 1207, 1441
BstMBI GATC 4 cut(s) 333, 741, 1204, 1438
BstMWI GCNNNNNNNGC 3 cut(s) 624, 764, 1500
BstNI CCWGG 1 cut(s) 1031
BstNSI RCATGY 2 cut(s) 268, 660
BstPI GGTNACC 1 cut(s) 962
BstSCI CCNGG 1 cut(s) 1029
BstSFI CTRYAG 1 cut(s) 700
BstSLI GKGCMC 2 cut(s) 137, 593
BstUI CGCG 1 cut(s) 725
BstV1I GCAGC 5 cut(s) 162, 446, 630, 686, 937
BstV2I GAAGAC 2 cut(s) 1294, 1530
BstX2I RGATCY 1 cut(s) 1438
BstYI RGATCY 1 cut(s) 1438
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 2 cut(s) 290, 758
BtgI CCRYGG 2 cut(s) 234, 1273
BtsCI GGATG 3 cut(s) 1253, 1269, 1303
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 6 cut(s) 141, 257, 458, 593, 709, 1284
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 3 cut(s) 49, 1239, 1381
CseI GACGC 2 cut(s) 714, 1492
Csp6I GTAC 2 cut(s) 93, 359
CviQI GTAC 2 cut(s) 93, 359
DdeI CTNAG 2 cut(s) 345, 1388
DpnI GATC 4 cut(s) 335, 743, 1206, 1440
DpnII GATC 4 cut(s) 333, 741, 1204, 1438
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 3 cut(s) 199, 308, 1371
EarI CTCTTC 3 cut(s) 199, 308, 1371
Eco130I CCWWGG 2 cut(s) 234, 1273
Eco24I GRGCYC 1 cut(s) 346
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 3 cut(s) 49, 1239, 1381
Eco57I CTGAAG 2 cut(s) 1288, 1524
Eco88I CYCGRG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 962
EcoO109I RGGNCCY 2 cut(s) 1239, 1381
EcoO65I GGTNACC 1 cut(s) 962
EcoRII CCWGG 1 cut(s) 1029
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 234, 1273
EcoT22I ATGCAT 2 cut(s) 1253, 1399
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 2 cut(s) 234, 1273
FalI AAGNNNNNCTT 2 cut(s) 1368, 1400
FaqI GGGAC 5 cut(s) 252, 401, 521, 1082, 1252
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 1 cut(s) 1525
Fnu4HI GCNGC 5 cut(s) 151, 435, 619, 700, 951
FokI GGATG 3 cut(s) 1256, 1260, 1310
FriOI GRGCYC 1 cut(s) 346
Fsp4HI GCNGC 5 cut(s) 151, 435, 619, 700, 951
FspBI CTAG 3 cut(s) 420, 1131, 1687
GlaI GCGC 1 cut(s) 72
GluI GCNGC 5 cut(s) 151, 435, 619, 700, 951
GsaI CCCAGC 1 cut(s) 699
GsuI CTGGAG 2 cut(s) 579, 1242
HaeIII GGCC 2 cut(s) 290, 758
HapII CCGG 2 cut(s) 497, 843
HgaI GACGC 2 cut(s) 714, 1492
HhaI GCGC 1 cut(s) 73
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HincII GTYRAC 2 cut(s) 296, 1449
HindII GTYRAC 2 cut(s) 296, 1449
HindIII AAGCTT 1 cut(s) 926
HpaII CCGG 2 cut(s) 497, 843
HphI GGTGA 3 cut(s) 857, 974, 1379
Hpy166II GTNNAC 5 cut(s) 296, 453, 591, 1449, 1526
Hpy188I TCNGA 7 cut(s) 822, 916, 1014, 1200, 1297, 1362, 1627
Hpy8I GTNNAC 5 cut(s) 296, 453, 591, 1449, 1526
HpyAV CCTTC 3 cut(s) 239, 709, 1394
HpyCH4III ACNGT 5 cut(s) 86, 450, 462, 1288, 1423
HpyCH4IV ACGT 1 cut(s) 1075
HpyF10VI GCNNNNNNNGC 3 cut(s) 624, 764, 1500
HpyF3I CTNAG 2 cut(s) 345, 1388
HpySE526I ACGT 1 cut(s) 1075
HspAI GCGC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 4 cut(s) 333, 741, 1204, 1438
LmnI GCTCC 4 cut(s) 64, 947, 1032, 1261
Lsp1109I GCAGC 5 cut(s) 162, 446, 630, 686, 937
LweI GCATC 5 cut(s) 421, 509, 625, 886, 1238
MaeI CTAG 3 cut(s) 420, 1131, 1687
MaeII ACGT 1 cut(s) 1075
MaeIII GTNAC 5 cut(s) 962, 985, 1076, 1118, 1570
MalI GATC 4 cut(s) 335, 743, 1206, 1440
MboI GATC 4 cut(s) 333, 741, 1204, 1438
MboII GAAGA 8 cut(s) 16, 186, 222, 295, 407, 1294, 1388, 1530
MfeI CAATTG 2 cut(s) 613, 622
MflI RGATCY 1 cut(s) 1438
MhlI GDGCHC 4 cut(s) 137, 346, 593, 1355
MluI ACGCGT 1 cut(s) 723
MlyI GAGTC 3 cut(s) 503, 1239, 1655
MmeI TCCRAC 6 cut(s) 95, 150, 210, 222, 677, 1388
Mph1103I ATGCAT 2 cut(s) 1253, 1399
MseI TTAA 5 cut(s) 377, 464, 485, 690, 1125
MslI CAYNNNNRTG 2 cut(s) 276, 752
MspA1I CMGCKG 2 cut(s) 104, 699
MspCI CTTAAG 1 cut(s) 484
MspI CCGG 2 cut(s) 497, 843
MspR9I CCNGG 1 cut(s) 1031
MunI CAATTG 2 cut(s) 613, 622
MvaI CCWGG 1 cut(s) 1031
MvnI CGCG 1 cut(s) 725
MwoI GCNNNNNNNGC 3 cut(s) 624, 764, 1500
NcoI CCATGG 2 cut(s) 234, 1273
NdeII GATC 4 cut(s) 333, 741, 1204, 1438
NlaIV GGNNCC 4 cut(s) 136, 1028, 1240, 1257
NmuCI GTSAC 3 cut(s) 962, 985, 1118
NsiI ATGCAT 2 cut(s) 1253, 1399
NspI RCATGY 2 cut(s) 268, 660
NspV TTCGAA 2 cut(s) 882, 1105
PaeR7I CTCGAG 1 cut(s) 367
PciI ACATGT 2 cut(s) 264, 656
PfeI GAWTC 8 cut(s) 817, 823, 863, 1094, 1159, 1565, 1622, 1672
PflFI GACNNNGTC 1 cut(s) 1645
PkrI GCNGC 5 cut(s) 152, 436, 620, 701, 952
PleI GAGTC 3 cut(s) 503, 1238, 1654
PpsI GAGTC 3 cut(s) 503, 1238, 1654
PpuMI RGGWCCY 2 cut(s) 1239, 1381
PscI ACATGT 2 cut(s) 264, 656
PsiI TTATAA 1 cut(s) 957
Psp5II RGGWCCY 2 cut(s) 1239, 1381
Psp6I CCWGG 1 cut(s) 1029
PspEI GGTNACC 1 cut(s) 962
PspFI CCCAGC 1 cut(s) 695
PspGI CCWGG 1 cut(s) 1029
PspN4I GGNNCC 4 cut(s) 136, 1028, 1240, 1257
PspPI GGNCC 3 cut(s) 49, 1239, 1381
PspPPI RGGWCCY 2 cut(s) 1239, 1381
PspXI VCTCGAGB 1 cut(s) 367
PstI CTGCAG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 1438
PsyI GACNNNGTC 1 cut(s) 1645
PvuII CAGCTG 2 cut(s) 104, 699
RsaI GTAC 2 cut(s) 94, 360
RsaNI GTAC 2 cut(s) 93, 359
RseI CAYNNNNRTG 2 cut(s) 276, 752
SaqAI TTAA 5 cut(s) 377, 464, 485, 690, 1125
SatI GCNGC 5 cut(s) 151, 435, 619, 700, 951
Sau3AI GATC 4 cut(s) 333, 741, 1204, 1438
Sau96I GGNCC 3 cut(s) 49, 1239, 1381
SchI GAGTC 3 cut(s) 503, 1239, 1655
ScrFI CCNGG 1 cut(s) 1031
SduI GDGCHC 4 cut(s) 137, 346, 593, 1355
SfaNI GCATC 5 cut(s) 421, 509, 625, 886, 1238
SfcI CTRYAG 1 cut(s) 700
Sfr274I CTCGAG 1 cut(s) 367
SfuI TTCGAA 2 cut(s) 882, 1105
SinI GGWCC 3 cut(s) 49, 1239, 1381
SlaI CTCGAG 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 276, 752
SmlI CTYRAG 5 cut(s) 10, 367, 484, 1212, 1325
SmoI CTYRAG 5 cut(s) 10, 367, 484, 1212, 1325
SspI AATATT 3 cut(s) 381, 524, 1601
SspMI CTAG 3 cut(s) 420, 1131, 1687
StyD4I CCNGG 1 cut(s) 1029
StyI CCWWGG 2 cut(s) 234, 1273
TaaI ACNGT 5 cut(s) 86, 450, 462, 1288, 1423
TaiI ACGT 1 cut(s) 1078
TaqI TCGA 4 cut(s) 368, 882, 1099, 1105
TatI WGTACW 1 cut(s) 92
TfiI GAWTC 8 cut(s) 817, 823, 863, 1094, 1159, 1565, 1622, 1672
Tru1I TTAA 5 cut(s) 377, 464, 485, 690, 1125
Tru9I TTAA 5 cut(s) 377, 464, 485, 690, 1125
TscAI CASTG 6 cut(s) 148, 264, 465, 593, 709, 1291
TseFI GTSAC 3 cut(s) 962, 985, 1118
TseI GCWGC 5 cut(s) 150, 434, 618, 699, 950
Tsp45I GTSAC 3 cut(s) 962, 985, 1118
TspDTI ATGAA 8 cut(s) 16, 414, 855, 933, 1172, 1389, 1415, 1635
TspRI CASTG 6 cut(s) 148, 264, 465, 593, 709, 1291
Tth111I GACNNNGTC 1 cut(s) 1645
Vha464I CTTAAG 1 cut(s) 484
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 3 cut(s) 49, 1239, 1381
XapI RAATTY 4 cut(s) 466, 648, 1015, 1513
XceI RCATGY 2 cut(s) 268, 660
XhoI CTCGAG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 1525
XspI CTAG 3 cut(s) 420, 1131, 1687
Zsp2I ATGCAT 2 cut(s) 1253, 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.