Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000086

Physical Location & Seq

Genomic Coordinates Forward (+)

13699147 .. 13702080

Gene Structure 2934 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1140400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGTTTCCTCAAGATTAATCAGATTATTGTTCCTTTGTACACAAATTTTGTTACTCATCATCAACGTTAATTTCACTGAAGCCCAGCTTGAACCCCCTGGACTTTCAGCATTCCAGTGTTCTAACGGCAAAGGTAACTACACCACTGGCAGCACCTATCAAACAAACCTCAAAAGCCTCCTCTCCACCTTATTCGATGCCAACAACAACAACGGCTACGGCTTTTACAATTCCTCCCTTGGCGTAAACAACGACAGAGTTTATGCAATCGGGCTCTGTCGAGGAGATATTCCAAAGGAAGCTTGCGGTAGCTGCCTTACTGACACTGCAAAAGGTCTCATGAATAATTGTACCAACCAGAAGGAGGCAATTGAATGGTTAGACAATTGTATGTTACGCTACTCAAACCGCTCCATATATGGCAGAAAGGAAATTAGTCCGGCTTACTACGCTTGGAATCCATCAAATGTATCCACGGGCTTGGATGCGTTCAATCAAGAGGTCTCTACCTTACTTAATGGACTACGACAAAAAGCAGCAACGGGTGGTGATCTTCGTAAATTTGCAACAGCAAACACAAGCATTCCAAGTTCTAATCTAAAAATATATGGACTTGTTCAGTGCACCCCAGAATTATCCGAGCAAAGTTGTATTGATTGCTTAAATTATGCTTTCAGAGTGTTTGAATCATGTTGTAGTGCATCAATTGGTATGAGAATTATGACACCAAGCTGTGGCGTAAGGTACGAAAATTACACCTTTTTTGACGTTACAACCGAGACACCATTACCTACACCACCGGCCGATACTGCGATTCCGGGAGGAAAGAAGAGTAATACCTCTCGGACTGTCATCATTACGGTTGTCACAGTTGTTTCCGTGGTACTAATTATATCGATCTGCATTTATCTGAGAGTGAAGAAGATGAAGGGAAAACTTGGAGAAGCAGATGAAATTCTTAATACAGAAGCCTTGCAGTTCGACCTTGCCTCCATAAGAACGGCTACAAATAACTTTTCTGAAGCCAACAAGCTTGGACGAGGTGGATTTGGTACTGTTTACAGGGGTAGGCTTTTGAACCAAGAAGATATAGCGGTTAAAAGGCTTTCTAGAGATTCTGCACAAGGAGATATAGAATTTAAAAATGAGGTCACATTAGTAGCCAAACTTCAACACCGCAATTTAGTAAGGCTTCTTGGTTTCTGCTTGGAAGGAAACGAAAGGGTTCTTATCTATGAGTTTGTCCCTAATGCAAGCCTCGATCATTTCATATTTGATCCAATCAAGCGCGAACATTTGGACTGGGATAGTCGCTACAAGATCATATTAGGAATTGGGCGAGGACTCCTTTACCTTCATGAAGATTCTCGTCTTAGAATAATTCATCGTGATATGAAAGCAAGTAACATTTTGTTAGATGCAGAAATGCTACCCAAAATTGCAGATTTTGGCATGGCAAGACTGTTTGATCTTGATCAAACACAAGGTGATACCAGTCGAGTTGTAGGGACTTATGGTTACATGGCACCTGAGTATGTAATGCGCGGACACTTTTCTGTTAAGTCGGACGTGTATAGCTTCGGTGTGTTAGTTTTGGAGATAATTACTGGACAGAAAAATAGTTCTTTCCGTCACGGTGGGAACGTGGAGGACCTTCTAAGCTATGCATGGAAAAGTTGGAAGGAGGACACAGCTTCAAATTTAATAGATCCCTTGTTGAAGAGTGGTTCAATACCAGAAATAATGAGATGCGTCCACATAGGATTATTATGTGTGCAACAAAATATAGCTGATAGGCCAACCATGGCTGCAGTTATTCTCATGCTTACTACCAACTCTCTTGATCTTCCAGTACCCTCACAACCAGCATTTTTTATGGATGGTGGCATTGAATCCTCCTCTGACACGTCTTTGGGATGGAAGGATAATTCTGGTGTGACAAGGTTAGGTCTATCTAAAAGTAGCTCTGTAAAATACCTGCTGCATGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0033993 GO:0036211 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0097305 GO:0098542 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

664

Amino Acids

73.36

Weight (kDa)

7.44

Isoelectric Point (pI)

36.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	39 - 137	4.2e-25	Salt stress response/antifungal
Stress-antifung	PF01657	162 - 252	2.8e-12	Salt stress response/antifungal
Pkinase	PF00069	340 - 542	2.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	342 - 606	1.7e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000086)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21230 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21410 AT4G21410 AT4G21410 AT4G38830
fragaria_vesca	FvH4_2g29861 FvH4_2g29861 FvH4_2g29870 FvH4_2g29870 FvH4_2g29870 FvH4_2g29871 FvH4_2g29871 FvH4_2g29875 FvH4_2g29878 FvH4_3g02833 FvH4_3g02833 FvH4_3g02834 FvH4_3g02870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g20010
malus_domestica	MD00G1101400.v1.1 MD00G1101700.v1.1 MD01G1027900.v1.1 MD02G1101400.v1.1 MD05G1336800.v1.1 MD05G1337000.v1.1 MD05G1337300.v1.1 MD05G1337400.v1.1 MD05G1337500.v1.1 MD07G1014000.v1.1 MD08G1139800.v1.1 MD08G1139900.v1.1 MD08G1140000.v1.1 MD08G1140100.v1.1 MD08G1140200.v1.1 MD08G1140400.v1.1 MD10G1312200.v1.1 MD10G1312500.v1.1 MD15G1117400.v1.1
prunus_persica	Prupe.1G174500_v2.0.a1 Prupe.1G468600_v2.0.a1 Prupe.1G469000_v2.0.a1 Prupe.1G469100_v2.0.a1 Prupe.1G469200_v2.0.a1 Prupe.1G469300_v2.0.a1 Prupe.4G027900_v2.0.a1 Prupe.4G028000_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028200_v2.0.a1 Prupe.4G028600_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028800_v2.0.a1 Prupe.4G100700_v2.0.a1 Prupe.6G183400_v2.0.a1 Prupe.6G183500_v2.0.a1 Prupe.6G183600_v2.0.a1 Prupe.6G184100_v2.0.a1 Prupe.6G184400_v2.0.a1 Prupe.6G184500_v2.0.a1
pyrus_communis	pycom01g05620 pycom01g05630 pycom05g30820 pycom05g30840 pycom05g30860 pycom05g30880 pycom05g30890 pycom05g30900 pycom08g11780 pycom08g11850 pycom10g26340 pycom10g26350 pycom10g26360 pycom10g26400 pycom10g26410 pycom15g10580
rosa_chinensis	RchiOBHm_Chr1g0313011 RchiOBHm_Chr1g0319461 RchiOBHm_Chr1g0319511 RchiOBHm_Chr2g0114611 RchiOBHm_Chr2g0114631 RchiOBHm_Chr2g0114691 RchiOBHm_Chr2g0114741 RchiOBHm_Chr2g0114751 RchiOBHm_Chr2g0114781 RchiOBHm_Chr2g0114791 RchiOBHm_Chr3g0494511 RchiOBHm_Chr3g0494521 RchiOBHm_Chr4g0413731 RchiOBHm_Chr5g0004271 RchiOBHm_Chr5g0004291 RchiOBHm_Chr5g0004301 RchiOBHm_Chr5g0004341 RchiOBHm_Chr5g0004351 RchiOBHm_Chr5g0004371 RchiOBHm_Chr5g0033301 RchiOBHm_Chr5g0033311 RchiOBHm_Chr5g0033341 RchiOBHm_Chr5g0033361 RchiOBHm_Chr5g0033391 RchiOBHm_Chr5g0033401 RchiOBHm_Chr5g0033431 RchiOBHm_Chr5g0033451 RchiOBHm_Chr5g0033461 RchiOBHm_Chr5g0033531 RchiOBHm_Chr5g0033541 RchiOBHm_Chr5g0033711 RchiOBHm_Chr5g0033851 RchiOBHm_Chr5g0033861 RchiOBHm_Chr5g0033871 RchiOBHm_Chr5g0054051 RchiOBHm_Chr5g0069631 RchiOBHm_Chr6g0265231 RchiOBHm_Chr6g0298941 RchiOBHm_Chr6g0298951 RchiOBHm_Chr6g0299001 RchiOBHm_Chr6g0299011 RchiOBHm_Chr6g0299021
rosa_laevigata	RLG00000008198 RLG00000010335 RLG00000011468 RLG00000011471 RLG00000014070 RLG00000014071 RLG00000018116 RLG00000018125 RLG00000031219 RLG00000031220 RLG00000031221 RLG00000031222 RLG00000031224 RLG00000033469 RLG00000033471 RLG00000033473 RLG00000033476 RLG00000033477 RLG00000033479 RLG00000033480 RLG00000033483 RLG00000035638
rosa_multiflora	Rmu_co8063382.1_g000001 Rmu_co8095246.1_g000001 Rmu_sc0000370.1_g000009 Rmu_sc0000370.1_g000019 Rmu_sc0000371.1_g000023 Rmu_sc0000371.1_g000036 Rmu_sc0000433.1_g000023 Rmu_sc0000433.1_g000024 Rmu_sc0000545.1_g000004 Rmu_sc0000981.1_g000001 Rmu_sc0001150.1_g000032 Rmu_sc0001242.1_g000004 Rmu_sc0001242.1_g000008 Rmu_sc0001522.1_g000035 Rmu_sc0002206.1_g000008 Rmu_sc0002206.1_g000021 Rmu_sc0002453.1_g000008 Rmu_sc0002453.1_g000018 Rmu_sc0003286.1_g000007 Rmu_sc0003286.1_g000013 Rmu_sc0004133.1_g000005 Rmu_sc0005660.1_g000005 Rmu_sc0005660.1_g000019 Rmu_sc0005660.1_g000025 Rmu_sc0005870.1_g000017 Rmu_sc0005870.1_g000022 Rmu_sc0006083.1_g000003 Rmu_sc0008092.1_g000016 Rmu_sc0008210.1_g000007 Rmu_sc0009715.1_g000001 Rmu_sc0011893.1_g000003 Rmu_sc0011893.1_g000004 Rmu_sc0015325.1_g000002 Rmu_sc0029922.1_g000001 Rmu_ssc0000359.1_g000017 Rmu_ssc0000424.1_g000009
rosa_roxburghii	Rroxscaffold_175G00432170 Rroxscaffold_1G00022110 Rroxscaffold_1G00046520 Rroxscaffold_1G00046550 Rroxscaffold_1G00046600 Rroxscaffold_1G00046650 Rroxscaffold_1G00046680 Rroxscaffold_1G00071130 Rroxscaffold_1G00071380 Rroxscaffold_1G00071410 Rroxscaffold_2G00130310 Rroxscaffold_2G00130320 Rroxscaffold_2G00130330 Rroxscaffold_4G00328340 Rroxscaffold_4G00328360 Rroxscaffold_4G00328370 Rroxscaffold_5G00357650 Rroxscaffold_7G00156890 Rroxscaffold_7G00169080 Rroxscaffold_7G00169090 Rroxscaffold_7G00169100 Rroxscaffold_7G00169170 Rroxscaffold_7G00169190
rosa_rugosa	Rorug01G0027900 Rorug01G0130600 Rorug02G0190700 Rorug04G0411000 Rorug04G0411000 Rorug04G0411200 Rorug04G0411300 Rorug04G0411400 Rorug04G0411500 Rorug05G0139300 Rorug05G0139500 Rorug05G0139800 Rorug05G0139900 Rorug05G0187000 Rorug05G0187000 Rorug05G0187000 Rorug05G0398900 Rorug06G0034600 Rorug06G0283900 Rorug06G0284000 Rorug06G0284100 Rorug06G0394700
rosa_samantha	Rh1BG036800 Rh1CG040700 Rh1CG040800 Rh1DG000200 Rh1DG028900 Rh1DG090600 Rh2AG248900 Rh2CG252600 Rh2CG253100 Rh2CG253500 Rh2CG253600 Rh2CG253900 Rh4DG177700 Rh5CG041500 Rh5CG041700 Rh5CG042100 Rh5CG260400 Rh5CG260500 Rh5CG260600 Rh5CG260900 Rh5CG261100 Rh5DG037300 Rh5DG037400 Rh5DG037500 Rh5DG236800 Rh5DG236900 Rh5DG237100 Rh5DG237500 Rh5DG237600 Rh5DG237700 Rh5DG241100 Rh6CG134500 Rh6CG409700 Rh6CG409800 Rh6CG410100 Rh6DG120900 Rh6DG396500 Rh6DG396600 Rh6DG396700 Rh6DG514400
rosa_wichuraiana	Rw1G003200 Rw1G003210 Rw1G003230 Rw2G019350 Rw4G015240 Rw5G003500 Rw5G003520 Rw5G003530 Rw5G003690 Rw5G003720 Rw5G003730 Rw5G021090 Rw5G021100 Rw5G021110 Rw5G021120 Rw5G021130 Rw6G011870 Rw6G034630 Rw7G038700

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1986
AccB1I	GGYRCC	1 cut(s)	1525
AccB7I	CCANNNNNTGG	1 cut(s)	734
AccBSI	CCGCTC	1 cut(s)	411
AccII	CGCG	2 cut(s)	1290, 1545
AciI	CCGC	5 cut(s)	306, 409, 1094, 1177, 1545
AclI	AACGTT	1 cut(s)	66
AclWI	GGATC	2 cut(s)	1271, 1703
AcoI	YGGCCR	1 cut(s)	801
AcsI	RAATTY	5 cut(s)	45, 560, 954, 1136, 1699
AcuI	CTGAAG	2 cut(s)	99, 1041
AdeI	CACNNNGTG	1 cut(s)	1487
AfaI	GTAC	6 cut(s)	40, 352, 746, 885, 1054, 1854
AfiI	CCNNNNNNNGG	2 cut(s)	364, 734
AflIII	ACRYGT	2 cut(s)	1569, 1905
AjiI	CACGTC	2 cut(s)	1570, 1908
AjnI	CCWGG	1 cut(s)	97
Alw21I	GWGCWC	1 cut(s)	626
Alw26I	GTCTC	3 cut(s)	341, 508, 773
Alw44I	GTGCAC	1 cut(s)	622
AlwI	GGATC	2 cut(s)	1271, 1703
AoxI	GGCC	2 cut(s)	801, 1796
ApaLI	GTGCAC	1 cut(s)	622
ApeKI	GCWGC	5 cut(s)	150, 312, 536, 1808, 1981
ApoI	RAATTY	5 cut(s)	45, 560, 954, 1136, 1699
AseI	ATTAAT	1 cut(s)	17
Asp700I	GAANNNNTTC	1 cut(s)	1224
AspLEI	GCGC	2 cut(s)	1290, 1545
AspS9I	GGNCC	1 cut(s)	1651
AsuC2I	CCSGG	1 cut(s)	819
AsuHPI	GGTGA	2 cut(s)	560, 1499
AvaII	GGWCC	1 cut(s)	1651
BaeGI	GKGCMC	1 cut(s)	626
BanI	GGYRCC	1 cut(s)	1525
BanII	GRGCYC	1 cut(s)	276
BarI	GAAGNNNNNNTAC	2 cut(s)	1152, 1184
Bbv12I	GWGCWC	1 cut(s)	626
BbvI	GCAGC	5 cut(s)	162, 299, 548, 1795, 1968
BccI	CCATC	3 cut(s)	469, 1874, 1911
BceAI	ACGGC	4 cut(s)	142, 229, 235, 1017
BciT130I	CCWGG	1 cut(s)	99
BciVI	GTATCC	1 cut(s)	481
BclI	TGATCA	1 cut(s)	1474
BcnI	CCSGG	1 cut(s)	819
BcoDI	GTCTC	3 cut(s)	341, 508, 773
BfaI	CTAG	1 cut(s)	1110
BfmI	CTRYAG	1 cut(s)	1809
BfuAI	ACCTGC	1 cut(s)	1986
BfuI	GTATCC	1 cut(s)	481
BisI	GCNGC	5 cut(s)	151, 313, 537, 1809, 1982
BlsI	GCNGC	5 cut(s)	152, 314, 538, 1810, 1983
Bme1390I	CCNGG	2 cut(s)	99, 819
Bme18I	GGWCC	1 cut(s)	1651
BmgBI	CACGTC	2 cut(s)	1570, 1908
BmgT120I	GGNCC	1 cut(s)	1651
BmiI	GGNNCC	1 cut(s)	1527
BmrFI	CCNGG	2 cut(s)	99, 819
BmrI	ACTGGG	1 cut(s)	1312
BmsI	GCATC	5 cut(s)	187, 475, 710, 1408, 1739
BmuI	ACTGGG	1 cut(s)	1312
BpuMI	CCSGG	1 cut(s)	819
Bsa29I	ATCGAT	1 cut(s)	896
BsaBI	GATNNNNATC	1 cut(s)	1473
BsaI	GGTCTC	2 cut(s)	341, 508
BsaJI	CCNNGG	5 cut(s)	97, 238, 474, 879, 1803
BsaXI	ACNNNNNCTCC	4 cut(s)	218, 248, 974, 1004
Bsc4I	CCNNNNNNNGG	2 cut(s)	364, 734
Bse118I	RCCGGY	1 cut(s)	799
Bse1I	ACTGG	6 cut(s)	115, 151, 1307, 1494, 1612, 1850
Bse8I	GATNNNNATC	1 cut(s)	1473
BseBI	CCWGG	1 cut(s)	99
BseCI	ATCGAT	1 cut(s)	896
BseDI	CCNNGG	5 cut(s)	97, 238, 474, 879, 1803
BseGI	GGATG	3 cut(s)	490, 1885, 1922
BseJI	GATNNNNATC	1 cut(s)	1473
BseLI	CCNNNNNNNGG	2 cut(s)	364, 734
BseMII	CTCAG	2 cut(s)	902, 1521
BseNI	ACTGG	6 cut(s)	115, 151, 1307, 1494, 1612, 1850
BseRI	GAGGAG	3 cut(s)	170, 297, 1888
BseSI	GKGCMC	1 cut(s)	626
BseX3I	CGGCCG	1 cut(s)	801
BseXI	GCAGC	5 cut(s)	162, 299, 548, 1795, 1968
BseYI	CCCAGC	1 cut(s)	84
BsgI	GTGCAG	1 cut(s)	1104
Bsh1236I	CGCG	2 cut(s)	1290, 1545
Bsh1285I	CGRYCG	1 cut(s)	804
BshFI	GGCC	2 cut(s)	803, 1798
BshNI	GGYRCC	1 cut(s)	1525
BshVI	ATCGAT	1 cut(s)	896
BsiEI	CGRYCG	1 cut(s)	804
BsiHKAI	GWGCWC	1 cut(s)	626
BsiSI	CCGG	3 cut(s)	440, 800, 818
BslFI	GGGAC	2 cut(s)	1229, 1522
BslI	CCNNNNNNNGG	2 cut(s)	364, 734
BsmAI	GTCTC	3 cut(s)	341, 508, 773
BsmFI	GGGAC	2 cut(s)	1229, 1522
BsmI	GAATGC	2 cut(s)	110, 582
BsnI	GGCC	2 cut(s)	803, 1798
Bso31I	GGTCTC	2 cut(s)	341, 508
Bsp1286I	GDGCHC	2 cut(s)	276, 626
Bsp1407I	TGTACA	1 cut(s)	38
Bsp143I	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
Bsp19I	CCATGG	1 cut(s)	1803
BspACI	CCGC	5 cut(s)	306, 409, 1094, 1177, 1545
BspANI	GGCC	2 cut(s)	803, 1798
BspCNI	CTCAG	2 cut(s)	903, 1522
BspDI	ATCGAT	1 cut(s)	896
BspFNI	CGCG	2 cut(s)	1290, 1545
BspHI	TCATGA	2 cut(s)	339, 1357
BspLI	GGNNCC	1 cut(s)	1527
BspMAI	CTGCAG	1 cut(s)	1813
BspMI	ACCTGC	1 cut(s)	1986
BspPI	GGATC	2 cut(s)	1271, 1703
BspT107I	GGYRCC	1 cut(s)	1525
BspTNI	GGTCTC	2 cut(s)	341, 508
BsrBI	CCGCTC	1 cut(s)	411
BsrFI	RCCGGY	1 cut(s)	799
BsrGI	TGTACA	1 cut(s)	38
BsrI	ACTGG	6 cut(s)	115, 151, 1307, 1494, 1612, 1850
BssAI	RCCGGY	1 cut(s)	799
BssECI	CCNNGG	5 cut(s)	97, 238, 474, 879, 1803
BssMI	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
BssT1I	CCWWGG	2 cut(s)	238, 1803
Bst2UI	CCWGG	1 cut(s)	99
Bst4CI	ACNGT	6 cut(s)	850, 862, 871, 1057, 1464, 1637
Bst6I	CTCTTC	2 cut(s)	824, 1715
BstAUI	TGTACA	1 cut(s)	38
BstC8I	GCNNGC	2 cut(s)	304, 1255
BstDEI	CTNAG	4 cut(s)	911, 1373, 1530, 1658
BstDSI	CCRYGG	3 cut(s)	474, 879, 1803
BstF5I	GGATG	3 cut(s)	490, 1885, 1922
BstFNI	CGCG	2 cut(s)	1290, 1545
BstHHI	GCGC	2 cut(s)	1290, 1545
BstKTI	GATC	9 cut(s)	553, 900, 1264, 1279, 1323, 1471, 1477, 1711, 1846
BstMAI	GTCTC	3 cut(s)	341, 508, 773
BstMBI	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
BstMCI	CGRYCG	1 cut(s)	804
BstMWI	GCNNNNNNNGC	3 cut(s)	312, 449, 809
BstNI	CCWGG	1 cut(s)	99
BstSCI	CCNGG	2 cut(s)	97, 817
BstSFI	CTRYAG	1 cut(s)	1809
BstSLI	GKGCMC	1 cut(s)	626
BstUI	CGCG	2 cut(s)	1290, 1545
BstV1I	GCAGC	5 cut(s)	162, 299, 548, 1795, 1968
BstX2I	RGATCY	1 cut(s)	1708
BstXI	CCANNNNNNTGG	1 cut(s)	481
BstYI	RGATCY	1 cut(s)	1708
BstZI	CGGCCG	1 cut(s)	801
Bsu15I	ATCGAT	1 cut(s)	896
BsuI	GTATCC	1 cut(s)	481
BsuRI	GGCC	2 cut(s)	803, 1798
BsuTUI	ATCGAT	1 cut(s)	896
BtgI	CCRYGG	3 cut(s)	474, 879, 1803
BtrI	CACGTC	2 cut(s)	1570, 1908
BtsCI	GGATG	3 cut(s)	490, 1885, 1922
BtsI	GCAGTG	1 cut(s)	324
BtsIMutI	CAGTG	5 cut(s)	75, 122, 144, 324, 626
BveI	ACCTGC	1 cut(s)	1986
Cac8I	GCNNGC	2 cut(s)	304, 1255
CciI	TCATGA	2 cut(s)	339, 1357
CfoI	GCGC	2 cut(s)	1290, 1545
Cfr10I	RCCGGY	1 cut(s)	799
Cfr13I	GGNCC	1 cut(s)	1651
ClaI	ATCGAT	1 cut(s)	896
CseI	GACGC	1 cut(s)	1741
Csp6I	GTAC	6 cut(s)	39, 351, 745, 884, 1053, 1853
CviAII	CATG	9 cut(s)	340, 690, 1358, 1453, 1522, 1668, 1804, 1822, 1985
CviQI	GTAC	6 cut(s)	39, 351, 745, 884, 1053, 1853
DdeI	CTNAG	4 cut(s)	911, 1373, 1530, 1658
DpnI	GATC	9 cut(s)	552, 899, 1263, 1278, 1322, 1470, 1476, 1710, 1845
DpnII	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
DraI	TTTAAA	1 cut(s)	1141
DraIII	CACNNNGTG	1 cut(s)	1487
EaeI	YGGCCR	1 cut(s)	801
EagI	CGGCCG	1 cut(s)	801
Eam1104I	CTCTTC	2 cut(s)	824, 1715
EarI	CTCTTC	2 cut(s)	824, 1715
EclXI	CGGCCG	1 cut(s)	801
Eco130I	CCWWGG	2 cut(s)	238, 1803
Eco24I	GRGCYC	1 cut(s)	276
Eco31I	GGTCTC	2 cut(s)	341, 508
Eco47I	GGWCC	1 cut(s)	1651
Eco52I	CGGCCG	1 cut(s)	801
Eco57I	CTGAAG	2 cut(s)	99, 1041
EcoO109I	RGGNCCY	1 cut(s)	1651
EcoRII	CCWGG	1 cut(s)	97
EcoT14I	CCWWGG	2 cut(s)	238, 1803
EcoT22I	ATGCAT	1 cut(s)	1669
EcoT38I	GRGCYC	1 cut(s)	276
ErhI	CCWWGG	2 cut(s)	238, 1803
FaeI	CATG	9 cut(s)	343, 693, 1361, 1456, 1525, 1671, 1807, 1825, 1988
FalI	AAGNNNNNCTT	2 cut(s)	72, 104
FaqI	GGGAC	2 cut(s)	1229, 1522
FatI	CATG	9 cut(s)	339, 689, 1357, 1452, 1521, 1667, 1803, 1821, 1984
FbaI	TGATCA	1 cut(s)	1474
Fnu4HI	GCNGC	5 cut(s)	151, 313, 537, 1809, 1982
FokI	GGATG	3 cut(s)	497, 1892, 1929
FriOI	GRGCYC	1 cut(s)	276
Fsp4HI	GCNGC	5 cut(s)	151, 313, 537, 1809, 1982
FspBI	CTAG	1 cut(s)	1110
GlaI	GCGC	2 cut(s)	1289, 1544
GluI	GCNGC	5 cut(s)	151, 313, 537, 1809, 1982
GsaI	CCCAGC	1 cut(s)	88
HaeIII	GGCC	2 cut(s)	803, 1798
HapII	CCGG	3 cut(s)	440, 800, 818
HgaI	GACGC	1 cut(s)	1741
HhaI	GCGC	2 cut(s)	1290, 1545
Hin1II	CATG	9 cut(s)	343, 693, 1361, 1456, 1525, 1671, 1807, 1825, 1988
Hin6I	GCGC	2 cut(s)	1288, 1543
HinP1I	GCGC	2 cut(s)	1288, 1543
HindIII	AAGCTT	2 cut(s)	300, 1031
HinfI	GANTC	7 cut(s)	457, 686, 814, 1115, 1344, 1364, 1892
HpaII	CCGG	3 cut(s)	440, 800, 818
HphI	GGTGA	2 cut(s)	560, 1499
Hpy166II	GTNNAC	5 cut(s)	41, 247, 624, 1060, 1756
Hpy188I	TCNGA	8 cut(s)	23, 640, 677, 846, 912, 1021, 1567, 1903
Hpy188III	TCNNGA	8 cut(s)	12, 340, 497, 1110, 1358, 1388, 1472, 1841
Hpy8I	GTNNAC	5 cut(s)	41, 247, 624, 1060, 1756
HpyAV	CCTTC	7 cut(s)	355, 922, 1205, 1364, 1664, 1675, 1915
HpyCH4III	ACNGT	6 cut(s)	850, 862, 871, 1057, 1464, 1637
HpyCH4IV	ACGT	5 cut(s)	66, 768, 1569, 1644, 1907
HpyF10VI	GCNNNNNNNGC	3 cut(s)	312, 449, 809
HpyF3I	CTNAG	4 cut(s)	911, 1373, 1530, 1658
HpySE526I	ACGT	5 cut(s)	66, 768, 1569, 1644, 1907
Hsp92II	CATG	9 cut(s)	343, 693, 1361, 1456, 1525, 1671, 1807, 1825, 1988
HspAI	GCGC	2 cut(s)	1288, 1543
Ksp22I	TGATCA	1 cut(s)	1474
Kzo9I	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
LmnI	GCTCC	1 cut(s)	416
Lsp1109I	GCAGC	5 cut(s)	162, 299, 548, 1795, 1968
LweI	GCATC	5 cut(s)	187, 475, 710, 1408, 1739
MaeI	CTAG	1 cut(s)	1110
MaeII	ACGT	5 cut(s)	66, 768, 1569, 1644, 1907
MalI	GATC	9 cut(s)	552, 899, 1263, 1278, 1322, 1470, 1476, 1710, 1845
MbiI	CCGCTC	1 cut(s)	411
MboI	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
MboII	GAAGA	8 cut(s)	545, 841, 931, 934, 1097, 1373, 1732, 1838
MfeI	CAATTG	3 cut(s)	369, 385, 705
MflI	RGATCY	1 cut(s)	1708
MhlI	GDGCHC	2 cut(s)	276, 626
MlyI	GAGTC	1 cut(s)	1338
MmeI	TCCRAC	2 cut(s)	1545, 1658
Mph1103I	ATGCAT	1 cut(s)	1669
MroXI	GAANNNNTTC	1 cut(s)	1224
MseI	TTAA	9 cut(s)	17, 69, 516, 662, 960, 1098, 1140, 1560, 1703
MslI	CAYNNNNRTG	1 cut(s)	115
MspI	CCGG	3 cut(s)	440, 800, 818
MspR9I	CCNGG	2 cut(s)	99, 819
MunI	CAATTG	3 cut(s)	369, 385, 705
Mva1269I	GAATGC	2 cut(s)	110, 582
MvaI	CCWGG	1 cut(s)	99
MvnI	CGCG	2 cut(s)	1290, 1545
MwoI	GCNNNNNNNGC	3 cut(s)	312, 449, 809
NciI	CCSGG	1 cut(s)	819
NcoI	CCATGG	1 cut(s)	1803
NdeII	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
NlaIII	CATG	9 cut(s)	343, 693, 1361, 1456, 1525, 1671, 1807, 1825, 1988
NlaIV	GGNNCC	1 cut(s)	1527
NmuCI	GTSAC	4 cut(s)	865, 1150, 1631, 1936
NsiI	ATGCAT	1 cut(s)	1669
PagI	TCATGA	2 cut(s)	339, 1357
PcsI	WCGNNNNNNNCGW	2 cut(s)	774, 1641
PctI	GAATGC	2 cut(s)	110, 582
PdmI	GAANNNNTTC	1 cut(s)	1224
PfeI	GAWTC	6 cut(s)	457, 686, 814, 1115, 1364, 1892
PflMI	CCANNNNNTGG	1 cut(s)	734
PfoI	TCCNGGA	1 cut(s)	817
PkrI	GCNGC	5 cut(s)	152, 314, 538, 1810, 1983
PleI	GAGTC	1 cut(s)	1338
PpsI	GAGTC	1 cut(s)	1338
PpuMI	RGGWCCY	1 cut(s)	1651
PshBI	ATTAAT	1 cut(s)	17
Psp1406I	AACGTT	1 cut(s)	66
Psp5II	RGGWCCY	1 cut(s)	1651
Psp6I	CCWGG	1 cut(s)	97
PspFI	CCCAGC	1 cut(s)	84
PspGI	CCWGG	1 cut(s)	97
PspN4I	GGNNCC	1 cut(s)	1527
PspPI	GGNCC	1 cut(s)	1651
PspPPI	RGGWCCY	1 cut(s)	1651
PstI	CTGCAG	1 cut(s)	1813
PsuI	RGATCY	1 cut(s)	1708
RsaI	GTAC	6 cut(s)	40, 352, 746, 885, 1054, 1854
RsaNI	GTAC	6 cut(s)	39, 351, 745, 884, 1053, 1853
RseI	CAYNNNNRTG	1 cut(s)	115
SaqAI	TTAA	9 cut(s)	17, 69, 516, 662, 960, 1098, 1140, 1560, 1703
SatI	GCNGC	5 cut(s)	151, 313, 537, 1809, 1982
Sau3AI	GATC	9 cut(s)	550, 897, 1261, 1276, 1320, 1468, 1474, 1708, 1843
Sau96I	GGNCC	1 cut(s)	1651
SchI	GAGTC	1 cut(s)	1338
ScrFI	CCNGG	2 cut(s)	99, 819
SduI	GDGCHC	2 cut(s)	276, 626
SfaNI	GCATC	5 cut(s)	187, 475, 710, 1408, 1739
SfcI	CTRYAG	1 cut(s)	1809
SinI	GGWCC	1 cut(s)	1651
SmiMI	CAYNNNNRTG	1 cut(s)	115
SmlI	CTYRAG	1 cut(s)	10
SmoI	CTYRAG	1 cut(s)	10
SsiI	CCGC	5 cut(s)	306, 409, 1094, 1177, 1545
SspMI	CTAG	1 cut(s)	1110
StyD4I	CCNGG	2 cut(s)	97, 817
StyI	CCWWGG	2 cut(s)	238, 1803
TaaI	ACNGT	6 cut(s)	850, 862, 871, 1057, 1464, 1637
TaiI	ACGT	5 cut(s)	69, 771, 1572, 1647, 1910
TaqI	TCGA	6 cut(s)	195, 280, 896, 981, 1260, 1498
TatI	WGTACW	1 cut(s)	38
TfiI	GAWTC	6 cut(s)	457, 686, 814, 1115, 1364, 1892
Tru1I	TTAA	9 cut(s)	17, 69, 516, 662, 960, 1098, 1140, 1560, 1703
Tru9I	TTAA	9 cut(s)	17, 69, 516, 662, 960, 1098, 1140, 1560, 1703
TscAI	CASTG	5 cut(s)	82, 122, 151, 331, 626
TseFI	GTSAC	4 cut(s)	865, 1150, 1631, 1936
TseI	GCWGC	5 cut(s)	150, 312, 536, 1808, 1981
Tsp45I	GTSAC	4 cut(s)	865, 1150, 1631, 1936
TspDTI	ATGAA	8 cut(s)	356, 941, 966, 1258, 1346, 1373, 1374, 1409
TspGWI	ACGGA	2 cut(s)	868, 1619
TspRI	CASTG	5 cut(s)	82, 122, 151, 331, 626
Van91I	CCANNNNNTGG	1 cut(s)	734
VneI	GTGCAC	1 cut(s)	622
VpaK11BI	GGWCC	1 cut(s)	1651
VspI	ATTAAT	1 cut(s)	17
XapI	RAATTY	5 cut(s)	45, 560, 954, 1136, 1699
XbaI	TCTAGA	1 cut(s)	1109
XmnI	GAANNNNTTC	1 cut(s)	1224
XspI	CTAG	1 cut(s)	1110
Zsp2I	ATGCAT	1 cut(s)	1669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.