RchiOBHm_Chr5g0004271

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000086
Physical Location & Seq
Forward (+)
2722937 .. 2725078
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28553

Sequence Viewer

Length: 1167 bp
ATGGACAAGGACAATTGCCGCAATTGCCTACAAGATGCTATCTCAAATATTCCAACATGTTGTGGTGGAAAGCAAGGAGGCAGAGTTCTTAAACCCAGCTGCAGTGTAAGGTTTGAGGCCACTCTTTTCTACGAGTCTACAGCCGATTCGGAAGTAAGTCTTCCACCAAAAAAAGTTTGGCAGATTCATTGCAATATGACTTTGAAACTATTAGAGATGCAACAGATGACTTCTCTGATGAAAATAAGCGGTGGATTTGGAGTTGTTTATAAGGGTCGTCTAGCTAATGGACAATATATAGCTGTCAAAAGGCTCTCAAAGAATTTTGAACAGGGTGACCGAGAGTTCAAAAATGAGGTCACATTAGTTGCTCAACTTCAACATCGGAATTTAGTAAGGCTCCTGGGATTTTGCTTGAAAATGAAGGAAAGGATCCTAATTTACGAATACGTGCCTAACACGAGTCCTAATCACTTCATCTTTGATCCAATCAATCATGGGCATTTGGATTGGGACACACGTTACAAAATCGTAGGAGGCATTGCTCGAGGGATTCTCTACCTCCATGAAGATTCTCGACTTCGAATTATCCATTGTGACCTTAAACCCAGCAATATTTTATTAGATGGAGATATGAATCCTAAAATTGCAGATTTTGGCTTGGCAAGATTATTTCTGATGGATCAAACTCATGGACACACAAACAGAGTCGCAGGGACCCATGGATACATGGCTCCAGAGTATGATAACCATGGGCGTTTCTCTGTCAAATCGGATGTCTACAGTCTTGGTGTGTTAGTTCTTGAGATCGTGAGTAGCAAGAAAGTGAGCACTTTTCGAAATGGTGAGAATGAACAGGACCTTCTCAGCTATGCATGGCGAAGTTGGAAAGAGGACACAGTTCATAATATCATAGATCCAATGTTGACACAGAGTTCAAGGATTGAAACAATGAGATGTATTCACATTGGCTTACTTTGCGTCCAAGAAAATCTGAATAGGAGACCAACTATGTCTTCAGTTGTTTCCATGCTTAATAGCAATTCTGTTACACTCTCACAACCATTGCGACCTGGATATTTTTTGCAATATAATGATGAATCAGACATCACCGAACTGACAGAGTCAATAAATGAGGCTTCAATTACGAATCCAGATCCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

388

Amino Acids

44.12

Weight (kDa)

7.61

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 83 - 342 3.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 83 - 345 2.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21230 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21400 AT4G21410 AT4G21410 AT4G21410 AT4G38830
fragaria_vesca FvH4_2g29861 FvH4_2g29861 FvH4_2g29870 FvH4_2g29870 FvH4_2g29870 FvH4_2g29871 FvH4_2g29871 FvH4_2g29875 FvH4_2g29878 FvH4_3g02833 FvH4_3g02833 FvH4_3g02834 FvH4_3g02870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g19870 FvH4_3g20010
malus_domestica MD00G1101400.v1.1 MD00G1101700.v1.1 MD01G1027900.v1.1 MD02G1101400.v1.1 MD05G1336800.v1.1 MD05G1337000.v1.1 MD05G1337300.v1.1 MD05G1337400.v1.1 MD05G1337500.v1.1 MD07G1014000.v1.1 MD08G1139800.v1.1 MD08G1139900.v1.1 MD08G1140000.v1.1 MD08G1140100.v1.1 MD08G1140200.v1.1 MD08G1140400.v1.1 MD10G1312200.v1.1 MD10G1312500.v1.1 MD15G1117400.v1.1
prunus_persica Prupe.1G174500_v2.0.a1 Prupe.1G468600_v2.0.a1 Prupe.1G469000_v2.0.a1 Prupe.1G469100_v2.0.a1 Prupe.1G469200_v2.0.a1 Prupe.1G469300_v2.0.a1 Prupe.4G027900_v2.0.a1 Prupe.4G028000_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028100_v2.0.a1 Prupe.4G028200_v2.0.a1 Prupe.4G028600_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028700_v2.0.a1 Prupe.4G028800_v2.0.a1 Prupe.4G100700_v2.0.a1 Prupe.6G183400_v2.0.a1 Prupe.6G183500_v2.0.a1 Prupe.6G183600_v2.0.a1 Prupe.6G184100_v2.0.a1 Prupe.6G184400_v2.0.a1 Prupe.6G184500_v2.0.a1
pyrus_communis pycom01g05620 pycom01g05630 pycom05g30820 pycom05g30840 pycom05g30860 pycom05g30880 pycom05g30890 pycom05g30900 pycom08g11780 pycom08g11850 pycom10g26340 pycom10g26350 pycom10g26360 pycom10g26400 pycom10g26410 pycom15g10580
rosa_chinensis RchiOBHm_Chr1g0313011 RchiOBHm_Chr1g0319461 RchiOBHm_Chr1g0319511 RchiOBHm_Chr2g0114611 RchiOBHm_Chr2g0114631 RchiOBHm_Chr2g0114691 RchiOBHm_Chr2g0114741 RchiOBHm_Chr2g0114751 RchiOBHm_Chr2g0114781 RchiOBHm_Chr2g0114791 RchiOBHm_Chr3g0494511 RchiOBHm_Chr3g0494521 RchiOBHm_Chr4g0413731 RchiOBHm_Chr5g0004271 RchiOBHm_Chr5g0004291 RchiOBHm_Chr5g0004301 RchiOBHm_Chr5g0004341 RchiOBHm_Chr5g0004351 RchiOBHm_Chr5g0004371 RchiOBHm_Chr5g0033301 RchiOBHm_Chr5g0033311 RchiOBHm_Chr5g0033341 RchiOBHm_Chr5g0033361 RchiOBHm_Chr5g0033391 RchiOBHm_Chr5g0033401 RchiOBHm_Chr5g0033431 RchiOBHm_Chr5g0033451 RchiOBHm_Chr5g0033461 RchiOBHm_Chr5g0033531 RchiOBHm_Chr5g0033541 RchiOBHm_Chr5g0033711 RchiOBHm_Chr5g0033851 RchiOBHm_Chr5g0033861 RchiOBHm_Chr5g0033871 RchiOBHm_Chr5g0054051 RchiOBHm_Chr5g0069631 RchiOBHm_Chr6g0265231 RchiOBHm_Chr6g0298941 RchiOBHm_Chr6g0298951 RchiOBHm_Chr6g0299001 RchiOBHm_Chr6g0299011 RchiOBHm_Chr6g0299021
rosa_laevigata RLG00000008198 RLG00000010335 RLG00000011468 RLG00000011471 RLG00000014070 RLG00000014071 RLG00000018116 RLG00000018125 RLG00000031219 RLG00000031220 RLG00000031221 RLG00000031222 RLG00000031224 RLG00000033469 RLG00000033471 RLG00000033473 RLG00000033476 RLG00000033477 RLG00000033479 RLG00000033480 RLG00000033483 RLG00000035638
rosa_multiflora Rmu_co8063382.1_g000001 Rmu_co8095246.1_g000001 Rmu_sc0000370.1_g000009 Rmu_sc0000370.1_g000019 Rmu_sc0000371.1_g000023 Rmu_sc0000371.1_g000036 Rmu_sc0000433.1_g000023 Rmu_sc0000433.1_g000024 Rmu_sc0000545.1_g000004 Rmu_sc0000981.1_g000001 Rmu_sc0001150.1_g000032 Rmu_sc0001242.1_g000004 Rmu_sc0001242.1_g000008 Rmu_sc0001522.1_g000035 Rmu_sc0002206.1_g000008 Rmu_sc0002206.1_g000021 Rmu_sc0002453.1_g000008 Rmu_sc0002453.1_g000018 Rmu_sc0003286.1_g000007 Rmu_sc0003286.1_g000013 Rmu_sc0004133.1_g000005 Rmu_sc0005660.1_g000005 Rmu_sc0005660.1_g000019 Rmu_sc0005660.1_g000025 Rmu_sc0005870.1_g000017 Rmu_sc0005870.1_g000022 Rmu_sc0006083.1_g000003 Rmu_sc0008092.1_g000016 Rmu_sc0008210.1_g000007 Rmu_sc0009715.1_g000001 Rmu_sc0011893.1_g000003 Rmu_sc0011893.1_g000004 Rmu_sc0015325.1_g000002 Rmu_sc0029922.1_g000001 Rmu_ssc0000359.1_g000017 Rmu_ssc0000424.1_g000009
rosa_roxburghii Rroxscaffold_175G00432170 Rroxscaffold_1G00022110 Rroxscaffold_1G00046520 Rroxscaffold_1G00046550 Rroxscaffold_1G00046600 Rroxscaffold_1G00046650 Rroxscaffold_1G00046680 Rroxscaffold_1G00071130 Rroxscaffold_1G00071380 Rroxscaffold_1G00071410 Rroxscaffold_2G00130310 Rroxscaffold_2G00130320 Rroxscaffold_2G00130330 Rroxscaffold_4G00328340 Rroxscaffold_4G00328360 Rroxscaffold_4G00328370 Rroxscaffold_5G00357650 Rroxscaffold_7G00156890 Rroxscaffold_7G00169080 Rroxscaffold_7G00169090 Rroxscaffold_7G00169100 Rroxscaffold_7G00169170 Rroxscaffold_7G00169190
rosa_rugosa Rorug01G0027900 Rorug01G0130600 Rorug02G0190700 Rorug04G0411000 Rorug04G0411000 Rorug04G0411200 Rorug04G0411300 Rorug04G0411400 Rorug04G0411500 Rorug05G0139300 Rorug05G0139500 Rorug05G0139800 Rorug05G0139900 Rorug05G0187000 Rorug05G0187000 Rorug05G0187000 Rorug05G0398900 Rorug06G0034600 Rorug06G0283900 Rorug06G0284000 Rorug06G0284100 Rorug06G0394700
rosa_samantha Rh1BG036800 Rh1CG040700 Rh1CG040800 Rh1DG000200 Rh1DG028900 Rh1DG090600 Rh2AG248900 Rh2CG252600 Rh2CG253100 Rh2CG253500 Rh2CG253600 Rh2CG253900 Rh4DG177700 Rh5CG041500 Rh5CG041700 Rh5CG042100 Rh5CG260400 Rh5CG260500 Rh5CG260600 Rh5CG260900 Rh5CG261100 Rh5DG037300 Rh5DG037400 Rh5DG037500 Rh5DG236800 Rh5DG236900 Rh5DG237100 Rh5DG237500 Rh5DG237600 Rh5DG237700 Rh5DG241100 Rh6CG134500 Rh6CG409700 Rh6CG409800 Rh6CG410100 Rh6DG120900 Rh6DG396500 Rh6DG396600 Rh6DG396700 Rh6DG514400
rosa_wichuraiana Rw1G003200 Rw1G003210 Rw1G003230 Rw2G019350 Rw4G015240 Rw5G003500 Rw5G003520 Rw5G003530 Rw5G003690 Rw5G003720 Rw5G003730 Rw5G021090 Rw5G021100 Rw5G021110 Rw5G021120 Rw5G021130 Rw6G011870 Rw6G034630 Rw7G038700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
AccI GTMKAC 2 cut(s) 137, 780
AciI CCGC 2 cut(s) 19, 249
AclWI GGATC 6 cut(s) 427, 440, 479, 690, 911, 1151
AcsI RAATTY 2 cut(s) 322, 388
AcuI CTGAAG 1 cut(s) 1002
AflIII ACRYGT 2 cut(s) 56, 518
AgsI TTSAA 8 cut(s) 205, 329, 349, 380, 418, 939, 947, 1143
AjnI CCWGG 2 cut(s) 402, 1072
AjuI GAANNNNNNNTTGG 2 cut(s) 1000, 1032
AloI GAACNNNNNNTCC 2 cut(s) 69, 101
AluBI AGCT 4 cut(s) 99, 284, 302, 870
AluI AGCT 4 cut(s) 99, 284, 302, 870
Alw21I GWGCWC 1 cut(s) 833
Alw26I GTCTC 1 cut(s) 997
AlwI GGATC 6 cut(s) 427, 440, 479, 690, 911, 1151
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 2 cut(s) 322, 388
ArsI GACNNNNNNTTYG 4 cut(s) 342, 374, 762, 794
AspS9I GGNCC 2 cut(s) 717, 859
AsuHPI GGTGA 3 cut(s) 347, 857, 1102
AsuII TTCGAA 2 cut(s) 583, 838
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 717, 859
BamHI GGATCC 1 cut(s) 432
BauI CACGAG 1 cut(s) 460
BbsI GAAGAC 2 cut(s) 152, 1008
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 1 cut(s) 86
BccI CCATC 2 cut(s) 620, 673
BciT130I CCWGG 2 cut(s) 404, 1074
BciVI GTATCC 1 cut(s) 719
BcoDI GTCTC 1 cut(s) 997
BfaI CTAG 2 cut(s) 281, 1165
BfmI CTRYAG 3 cut(s) 100, 138, 781
BfuI GTATCC 1 cut(s) 719
BisI GCNGC 2 cut(s) 19, 100
BlsI GCNGC 2 cut(s) 20, 101
Bme1390I CCNGG 2 cut(s) 404, 1074
Bme18I GGWCC 2 cut(s) 717, 859
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 717, 859
BmiI GGNNCC 5 cut(s) 401, 434, 718, 719, 735
BmrFI CCNGG 2 cut(s) 404, 1074
BmsI GCATC 2 cut(s) 25, 207
BpiI GAAGAC 2 cut(s) 152, 1008
BplI GAGNNNNNCTC 2 cut(s) 540, 572
BpmI CTGGAG 1 cut(s) 720
Bpu14I TTCGAA 2 cut(s) 583, 838
BpuEI CTTGAG 1 cut(s) 824
BsaAI YACGTR 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 997
BsaJI CCNNGG 3 cut(s) 403, 721, 751
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bse3DI GCAATG 3 cut(s) 187, 540, 1064
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 2 cut(s) 404, 1074
BseDI CCNNGG 3 cut(s) 403, 721, 751
BseGI GGATG 1 cut(s) 781
BseJI GATNNNNATC 1 cut(s) 636
BseMI GCAATG 3 cut(s) 187, 540, 1064
BseMII CTCAG 1 cut(s) 880
BseXI GCAGC 1 cut(s) 86
BseYI CCCAGC 2 cut(s) 95, 608
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 833
BsiHKCI CYCGRG 1 cut(s) 546
BslFI GGGAC 2 cut(s) 527, 730
BsmAI GTCTC 1 cut(s) 997
BsmFI GGGAC 2 cut(s) 527, 730
BsnI GGCC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 997
BsoBI CYCGRG 1 cut(s) 546
Bsp119I TTCGAA 2 cut(s) 583, 838
Bsp1286I GDGCHC 1 cut(s) 833
Bsp143I GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
Bsp19I CCATGG 2 cut(s) 721, 751
BspACI CCGC 2 cut(s) 19, 249
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 879
BspLI GGNNCC 5 cut(s) 401, 434, 718, 719, 735
BspMAI CTGCAG 1 cut(s) 104
BspPI GGATC 6 cut(s) 427, 440, 479, 690, 911, 1151
BspT104I TTCGAA 2 cut(s) 583, 838
BspTNI GGTCTC 1 cut(s) 997
BsrDI GCAATG 3 cut(s) 187, 540, 1064
BssECI CCNNGG 3 cut(s) 403, 721, 751
BssMI GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
BssSI CACGAG 1 cut(s) 460
BssT1I CCWWGG 2 cut(s) 721, 751
Bst2BI CACGAG 1 cut(s) 460
Bst2UI CCWGG 2 cut(s) 404, 1074
Bst4CI ACNGT 2 cut(s) 785, 901
BstBAI YACGTR 1 cut(s) 451
BstBI TTCGAA 2 cut(s) 583, 838
BstDEI CTNAG 1 cut(s) 866
BstDSI CCRYGG 2 cut(s) 721, 751
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 1 cut(s) 781
BstKTI GATC 6 cut(s) 435, 487, 685, 810, 919, 1159
BstMAI GTCTC 1 cut(s) 997
BstMBI GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
BstMWI GCNNNNNNNGC 2 cut(s) 24, 978
BstNI CCWGG 2 cut(s) 404, 1074
BstNSI RCATGY 1 cut(s) 60
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 402, 1072
BstSFI CTRYAG 3 cut(s) 100, 138, 781
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 2 cut(s) 152, 1008
BstX2I RGATCY 3 cut(s) 432, 916, 1156
BstYI RGATCY 3 cut(s) 432, 916, 1156
BsuI GTATCC 1 cut(s) 719
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 2 cut(s) 721, 751
BtsCI GGATG 1 cut(s) 781
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 717, 859
CseI GACGC 1 cut(s) 970
CviAII CATG 9 cut(s) 57, 497, 566, 692, 722, 730, 752, 876, 1030
DdeI CTNAG 1 cut(s) 866
DpnI GATC 6 cut(s) 434, 486, 684, 809, 918, 1158
DpnII GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
Eco130I CCWWGG 2 cut(s) 721, 751
Eco31I GGTCTC 1 cut(s) 997
Eco47I GGWCC 2 cut(s) 717, 859
Eco57I CTGAAG 1 cut(s) 1002
Eco88I CYCGRG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 335
EcoO109I RGGNCCY 2 cut(s) 717, 859
EcoO65I GGTNACC 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 402, 1072
EcoT14I CCWWGG 2 cut(s) 721, 751
EcoT22I ATGCAT 1 cut(s) 877
ErhI CCWWGG 2 cut(s) 721, 751
FaeI CATG 9 cut(s) 60, 500, 569, 695, 725, 733, 755, 879, 1033
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 2 cut(s) 527, 730
FatI CATG 9 cut(s) 56, 496, 565, 691, 721, 729, 751, 875, 1029
FblI GTMKAC 2 cut(s) 137, 780
Fnu4HI GCNGC 2 cut(s) 19, 100
FokI GGATG 1 cut(s) 788
Fsp4HI GCNGC 2 cut(s) 19, 100
FspBI CTAG 2 cut(s) 281, 1165
GluI GCNGC 2 cut(s) 19, 100
GsaI CCCAGC 2 cut(s) 99, 612
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 1 cut(s) 119
HgaI GACGC 1 cut(s) 970
Hin1II CATG 9 cut(s) 60, 500, 569, 695, 725, 733, 755, 879, 1033
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HphI GGTGA 3 cut(s) 347, 857, 1102
Hpy166II GTNNAC 3 cut(s) 138, 781, 927
Hpy188I TCNGA 7 cut(s) 151, 237, 387, 678, 775, 996, 1105
Hpy188III TCNNGA 5 cut(s) 576, 737, 803, 811, 1154
Hpy8I GTNNAC 3 cut(s) 138, 781, 927
HpyAV CCTTC 2 cut(s) 418, 872
HpyCH4III ACNGT 2 cut(s) 785, 901
HpyCH4IV ACGT 2 cut(s) 450, 520
HpyCH4V TGCA 6 cut(s) 102, 192, 220, 650, 875, 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 978
HpyF3I CTNAG 1 cut(s) 866
HpySE526I ACGT 2 cut(s) 450, 520
Hsp92II CATG 9 cut(s) 60, 500, 569, 695, 725, 733, 755, 879, 1033
KflI GGGWCCC 1 cut(s) 717
Kzo9I GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
LmnI GCTCC 2 cut(s) 405, 739
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 2 cut(s) 25, 207
MaeI CTAG 2 cut(s) 281, 1165
MaeII ACGT 2 cut(s) 450, 520
MaeIII GTNAC 5 cut(s) 335, 358, 521, 596, 1048
MalI GATC 6 cut(s) 434, 486, 684, 809, 918, 1158
MboI GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
MboII GAAGA 3 cut(s) 152, 581, 1008
MfeI CAATTG 2 cut(s) 13, 22
MflI RGATCY 3 cut(s) 432, 916, 1156
MhlI GDGCHC 1 cut(s) 833
MluCI AATT 9 cut(s) 13, 22, 322, 388, 438, 585, 645, 1042, 1143
MlyI GAGTC 4 cut(s) 143, 472, 717, 1133
MmeI TCCRAC 2 cut(s) 77, 866
MnlI CCTC 8 cut(s) 71, 109, 349, 530, 542, 572, 886, 1129
Mph1103I ATGCAT 1 cut(s) 877
MseI TTAA 3 cut(s) 90, 603, 1035
MspA1I CMGCKG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 404, 1074
MunI CAATTG 2 cut(s) 13, 22
MvaI CCWGG 2 cut(s) 404, 1074
MwoI GCNNNNNNNGC 2 cut(s) 24, 978
NcoI CCATGG 2 cut(s) 721, 751
NdeII GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
NlaIII CATG 9 cut(s) 60, 500, 569, 695, 725, 733, 755, 879, 1033
NlaIV GGNNCC 5 cut(s) 401, 434, 718, 719, 735
NmuCI GTSAC 3 cut(s) 335, 358, 596
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 1 cut(s) 60
NspV TTCGAA 2 cut(s) 583, 838
PaeR7I CTCGAG 1 cut(s) 546
PciI ACATGT 1 cut(s) 56
PfeI GAWTC 7 cut(s) 146, 184, 553, 572, 637, 1100, 1150
PflFI GACNNNGTC 1 cut(s) 1123
PkrI GCNGC 2 cut(s) 20, 101
PleI GAGTC 4 cut(s) 142, 471, 716, 1132
PpsI GAGTC 4 cut(s) 142, 471, 716, 1132
Ppu21I YACGTR 1 cut(s) 451
PpuMI RGGWCCY 2 cut(s) 717, 859
PscI ACATGT 1 cut(s) 56
PsiI TTATAA 1 cut(s) 270
Psp5II RGGWCCY 2 cut(s) 717, 859
Psp6I CCWGG 2 cut(s) 402, 1072
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 2 cut(s) 95, 608
PspGI CCWGG 2 cut(s) 402, 1072
PspN4I GGNNCC 5 cut(s) 401, 434, 718, 719, 735
PspPI GGNCC 2 cut(s) 717, 859
PspPPI RGGWCCY 2 cut(s) 717, 859
PspXI VCTCGAGB 1 cut(s) 546
PstI CTGCAG 1 cut(s) 104
PsuI RGATCY 3 cut(s) 432, 916, 1156
PsyI GACNNNGTC 1 cut(s) 1123
PvuII CAGCTG 1 cut(s) 99
SaqAI TTAA 3 cut(s) 90, 603, 1035
SatI GCNGC 2 cut(s) 19, 100
Sau3AI GATC 6 cut(s) 432, 484, 682, 807, 916, 1156
Sau96I GGNCC 2 cut(s) 717, 859
SchI GAGTC 4 cut(s) 143, 472, 717, 1133
ScrFI CCNGG 2 cut(s) 404, 1074
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 2 cut(s) 25, 207
SfcI CTRYAG 3 cut(s) 100, 138, 781
Sfr274I CTCGAG 1 cut(s) 546
SfuI TTCGAA 2 cut(s) 583, 838
SinI GGWCC 2 cut(s) 717, 859
SlaI CTCGAG 1 cut(s) 546
SmlI CTYRAG 2 cut(s) 546, 803
SmoI CTYRAG 2 cut(s) 546, 803
Sse9I AATT 9 cut(s) 13, 22, 322, 388, 438, 585, 645, 1042, 1143
SsiI CCGC 2 cut(s) 19, 249
SspI AATATT 2 cut(s) 49, 616
SspMI CTAG 2 cut(s) 281, 1165
StyD4I CCNGG 2 cut(s) 402, 1072
StyI CCWWGG 2 cut(s) 721, 751
TaaI ACNGT 2 cut(s) 785, 901
TaiI ACGT 2 cut(s) 453, 523
TaqI TCGA 4 cut(s) 547, 577, 583, 838
TaqII GACCGA 1 cut(s) 354
TasI AATT 9 cut(s) 13, 22, 322, 388, 438, 585, 645, 1042, 1143
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 7 cut(s) 146, 184, 553, 572, 637, 1100, 1150
Tru1I TTAA 3 cut(s) 90, 603, 1035
Tru9I TTAA 3 cut(s) 90, 603, 1035
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 3 cut(s) 335, 358, 596
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 3 cut(s) 335, 358, 596
TspDTI ATGAA 9 cut(s) 176, 254, 437, 466, 582, 650, 867, 893, 1113
TspRI CASTG 1 cut(s) 109
Tth111I GACNNNGTC 1 cut(s) 1123
VpaK11BI GGWCC 2 cut(s) 717, 859
XapI RAATTY 2 cut(s) 322, 388
XceI RCATGY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XhoI CTCGAG 1 cut(s) 546
XmiI GTMKAC 2 cut(s) 137, 780
XspI CTAG 2 cut(s) 281, 1165
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.