MD02G1297800.v1.1
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000326
Physical Location & Seq
Forward (+)
35282588 .. 35283700
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1297800.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGGGTCGCGGGAGGCTGAATATGGAACTCATAACGAACGAAAGAGCTCGAAAAGCAACGTTTCAGAAGAGAACGAAGGGAATTCTGAAGAAGGCCGGCGAGATTTCAACTCTTTGTGACCTAGATGTGTGCATGATCATCTATGGCCCGAAACAAGCTGGTCGGAATCCCGAACTCCACACGTGGCCAAGAAACCCGAGTGAGGTCAATCGCATAATCAACAAGTACAAGGCAAGCACCACGTGCAAACCAGCCAAGAAAACCTTCAATTTGTCCGACCTGTTGATGGATCGAAAGACAAAGGTACATGTCGATACCTACAGAGCGCGCAAGGAGATGTACGAGGCCAAGTACCCAACATGGGATGAACGTATTGAAAATTTCTCCGAAAATGAACTGGAGCTGCTTCTGAATGCATTGGATGCTAAGCTCGAGGCTGGCAAGAGAACATTACTTCATAAGAGGAACCGGCCTGCTGAACACCAGTATATTTGTTCATCAACCCAAAGGCAGCCTTGTAATTATTACAAACCGGATCAAAATGATAACAATGTGGAGGACAAAAATCCTATGACCAGCTGCTCTTGGAATCGTAGTAATATGGATATGGTGGAGATCCTCCAGCCCTTTCCAGTCTCATCATTTGACCATCATCAGCAGCAGCAGCACCCTTCCGATCAAATGCTTCGATTCGATAATTCAAATGAGTATGTGAATAGCGTTTTGGCTCCTAACAATAATCCCGGTCCGCTGGCGATGTGGATGCTGATGCAGAGCAAATACAACTATAGTAGTCTCCAGTTAACCGGAGGGGCTTCCGGCAGTAATTCTCAATTACCATTTGAAGGGCGGCATCACAGTAACTTCAATAACCCGATGATGATTCAGTCTACGGTGGAGAATATGAATATGAATATGATGAGGCAGGAGTACTATTCAGCTCGACTCATGCAGCAGCCAGCTGTGCCCTACTTGCAGTATCCAGTGCTGCCGGCTAGCGTTTCATCATCTCATCAAGTTCATCAAGTGCAGGCTTCTCATCAGGTAGTGAGAGACGATGATCAGTACGAAGATATCAGCCAGTATCTAGTCATGAATAACAAGATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04022 ko04371 ko05418 map04013 map04022 map04371 map05418
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

371

Amino Acids

42.88

Weight (kDa)

8.72

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 11 - 51 5.1e-17 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18650 AT4G02235 AT4G11250 AT5G26865 AT5G26950 AT5G27050 AT5G27070 AT5G27090 AT5G27090 AT5G27580 AT5G27944 AT5G37415 AT5G37415 AT5G38740 AT5G39750 AT5G39810 AT5G40070 AT5G40120 AT5G40220 AT5G41200 AT5G55690 AT5G55690 AT5G58890 AT5G65330
fragaria_vesca FvH4_2g27520 FvH4_3g19300 FvH4_4g07700 FvH4_4g07700 FvH4_4g07700 FvH4_5g27070 FvH4_6g53490 FvH4_7g04141
malus_domestica MD02G1297800.v1.1 MD15G1430800.v1.1 MD17G1250500.v1.1
prunus_persica Prupe.2G110900_v2.0.a1 Prupe.3G148900_v2.0.a1 Prupe.3G306000_v2.0.a1 Prupe.7G030200_v2.0.a1 Prupe.7G030800_v2.0.a1 Prupe.7G030800_v2.0.a1 Prupe.7G030800_v2.0.a1 Prupe.7G031800_v2.0.a1 Prupe.7G031800_v2.0.a1 Prupe.7G032000_v2.0.a1
pyrus_communis pycom02g25070 pycom06g01170 pycom09g17250 pycom111g00930 pycom15g38080
rosa_chinensis RchiOBHm_Chr2g0175531 RchiOBHm_Chr5g0016461 RchiOBHm_Chr6g0296351
rosa_laevigata RLG00000002414 RLG00000002417 RLG00000009181 RLG00000011703 RLG00000022336 RLG00000029928 RLG00000029934
rosa_multiflora Rmu_co8046860.1_g000001 Rmu_sc0000215.1_g000011 Rmu_sc0000215.1_g000020 Rmu_sc0000379.1_g000004 Rmu_sc0001478.1_g000006 Rmu_sc0002636.1_g000029 Rmu_sc0009440.1_g000006
rosa_roxburghii Rroxscaffold_2G00077230 Rroxscaffold_3G00241440 Rroxscaffold_5G00345600 Rroxscaffold_7G00171600 Rroxscaffold_7G00178500
rosa_rugosa Rorug02G0585900 Rorug04G0025700 Rorug06G0135200 Rorug06G0261200 Rorug06G0261300 Rorug06G0261400 Rorug07G0173300
rosa_samantha Rh1AG091800 Rh1AG093000 Rh1BG072700 Rh1CG088300 Rh1CG089800 Rh1CG090200 Rh1DG095900 Rh2AG665100 Rh2BG675900 Rh2CG639100 Rh2DG689900 Rh4AG103500 Rh4BG098600 Rh4CG111800 Rh4DG095500 Rh5AG126100 Rh5CG137400 Rh5DG124800 Rh5DG124900 Rh6AG373100 Rh6BG381200 Rh6CG386500 Rh6DG374000 Rh7AG315800 Rh7BG306000 Rh7CG333200 Rh7DG314000 Rh7DG314500
rosa_wichuraiana Rw2G054520 Rw4G008380 Rw5G010930 Rw6G032530 Rw7G026670 Rw7G026760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 890
AccII CGCG 2 cut(s) 9, 328
AciI CCGC 3 cut(s) 9, 749, 850
AclI AACGTT 1 cut(s) 59
AclWI GGATC 3 cut(s) 297, 543, 610
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 2 cut(s) 81, 379
AcuI CTGAAG 1 cut(s) 107
AcvI CACGTG 2 cut(s) 183, 243
AdeI CACNNNGTG 1 cut(s) 243
AfaI GTAC 6 cut(s) 227, 306, 341, 353, 932, 1067
AfiI CCNNNNNNNGG 4 cut(s) 202, 286, 361, 845
AflIII ACRYGT 2 cut(s) 180, 307
AgsI TTSAA 6 cut(s) 108, 268, 377, 702, 845, 868
AjuI GAANNNNNNNTTGG 4 cut(s) 248, 280, 707, 739
AluBI AGCT 7 cut(s) 47, 158, 403, 430, 579, 941, 962
AluI AGCT 7 cut(s) 47, 158, 403, 430, 579, 941, 962
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 3 cut(s) 640, 800, 1047
AlwI GGATC 3 cut(s) 297, 543, 610
Ama87I CYCGRG 2 cut(s) 196, 431
AoxI GGCC 5 cut(s) 93, 145, 185, 345, 470
ApeKI GCWGC 9 cut(s) 403, 511, 579, 658, 661, 664, 952, 955, 988
ApoI RAATTY 2 cut(s) 81, 379
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 328, 330
AspS9I GGNCC 2 cut(s) 146, 746
AsuC2I CCSGG 1 cut(s) 744
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 2 cut(s) 196, 431
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 969
BalI TGGCCA 1 cut(s) 187
BanII GRGCYC 1 cut(s) 49
BbrPI CACGTG 2 cut(s) 183, 243
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 9 cut(s) 390, 523, 566, 670, 673, 676, 964, 967, 975
BccI CCATC 3 cut(s) 280, 657, 1099
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BciVI GTATCC 1 cut(s) 990
BclI TGATCA 2 cut(s) 135, 1060
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 640, 800, 1047
BfaI CTAG 3 cut(s) 122, 996, 1088
BfmI CTRYAG 2 cut(s) 319, 787
BfuI GTATCC 1 cut(s) 990
BlpI GCTNAGC 1 cut(s) 426
BmcAI AGTACT 1 cut(s) 932
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 1 cut(s) 746
BmeT110I CYCGRG 2 cut(s) 196, 431
BmgT120I GGNCC 2 cut(s) 146, 746
BmiI GGNNCC 2 cut(s) 467, 729
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 4 cut(s) 412, 753, 759, 862
BmtI GCTAGC 1 cut(s) 999
BpmI CTGGAG 3 cut(s) 419, 605, 782
Bpu1102I GCTNAGC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 2 cut(s) 183, 243
BsaWI WCCGGW 2 cut(s) 532, 806
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 286, 361, 845
Bse118I RCCGGY 3 cut(s) 95, 468, 991
Bse1I ACTGG 6 cut(s) 402, 484, 632, 799, 983, 1081
BseGI GGATG 3 cut(s) 370, 427, 768
BseLI CCNNNNNNNGG 4 cut(s) 202, 286, 361, 845
BseNI ACTGG 6 cut(s) 402, 484, 632, 799, 983, 1081
BsePI GCGCGC 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 969
BseXI GCAGC 9 cut(s) 390, 523, 566, 670, 673, 676, 964, 967, 975
BsgI GTGCAG 1 cut(s) 1049
Bsh1236I CGCG 2 cut(s) 9, 328
BshFI GGCC 5 cut(s) 95, 147, 187, 347, 472
BsiHKAI GWGCWC 1 cut(s) 49
BsiHKCI CYCGRG 2 cut(s) 196, 431
BsiSI CCGG 7 cut(s) 96, 469, 533, 744, 807, 819, 992
BslI CCNNNNNNNGG 4 cut(s) 202, 286, 361, 845
BsmAI GTCTC 3 cut(s) 640, 800, 1047
BsmBI CGTCTC 1 cut(s) 1047
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 5 cut(s) 95, 147, 187, 347, 472
BsoBI CYCGRG 2 cut(s) 196, 431
Bsp1286I GDGCHC 2 cut(s) 49, 969
Bsp143I GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 3 cut(s) 9, 749, 850
BspANI GGCC 5 cut(s) 95, 147, 187, 347, 472
BspFNI CGCG 2 cut(s) 9, 328
BspHI TCATGA 1 cut(s) 1092
BspLI GGNNCC 2 cut(s) 467, 729
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 3 cut(s) 297, 543, 610
BsrFI RCCGGY 3 cut(s) 95, 468, 991
BsrI ACTGG 6 cut(s) 402, 484, 632, 799, 983, 1081
BssAI RCCGGY 3 cut(s) 95, 468, 991
BssHII GCGCGC 1 cut(s) 326
BssMI GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
Bst4CI ACNGT 2 cut(s) 860, 895
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 243, 422
BstBAI YACGTR 2 cut(s) 183, 243
BstDEI CTNAG 1 cut(s) 426
BstF5I GGATG 3 cut(s) 370, 427, 768
BstFNI CGCG 2 cut(s) 9, 328
BstHHI GCGC 2 cut(s) 328, 330
BstKTI GATC 6 cut(s) 138, 292, 538, 618, 679, 1063
BstMAI GTCTC 3 cut(s) 640, 800, 1047
BstMBI GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
BstMWI GCNNNNNNNGC 6 cut(s) 53, 243, 422, 664, 964, 973
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 2 cut(s) 319, 787
BstSLI GKGCMC 1 cut(s) 969
BstUI CGCG 2 cut(s) 9, 328
BstV1I GCAGC 9 cut(s) 390, 523, 566, 670, 673, 676, 964, 967, 975
BstX2I RGATCY 1 cut(s) 615
BstYI RGATCY 1 cut(s) 615
BsuI GTATCC 1 cut(s) 990
BsuRI GGCC 5 cut(s) 95, 147, 187, 347, 472
BtgZI GCGATG 1 cut(s) 770
BtsCI GGATG 3 cut(s) 370, 427, 768
BtsIMutI CAGTG 1 cut(s) 990
CciI TCATGA 1 cut(s) 1092
CfoI GCGC 2 cut(s) 328, 330
Cfr10I RCCGGY 3 cut(s) 95, 468, 991
Cfr13I GGNCC 2 cut(s) 146, 746
CpoI CGGWCCG 1 cut(s) 746
Csp6I GTAC 6 cut(s) 226, 305, 340, 352, 931, 1066
CspI CGGWCCG 1 cut(s) 746
CviAII CATG 5 cut(s) 133, 308, 360, 949, 1093
CviQI GTAC 6 cut(s) 226, 305, 340, 352, 931, 1066
DdeI CTNAG 1 cut(s) 426
DpnI GATC 6 cut(s) 137, 291, 537, 617, 678, 1062
DpnII GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
DraIII CACNNNGTG 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 1 cut(s) 746
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 107
Eco72I CACGTG 2 cut(s) 183, 243
Eco88I CYCGRG 2 cut(s) 196, 431
EcoICRI GAGCTC 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 81
EcoRV GATATC 1 cut(s) 1075
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 49
Esp3I CGTCTC 1 cut(s) 1047
FaeI CATG 5 cut(s) 136, 311, 363, 952, 1096
FalI AAGNNNNNCTT 4 cut(s) 248, 280, 499, 531
FatI CATG 5 cut(s) 132, 307, 359, 948, 1092
FauI CCCGC 1 cut(s) 2
FbaI TGATCA 2 cut(s) 135, 1060
FblI GTMKAC 1 cut(s) 890
FokI GGATG 3 cut(s) 377, 434, 775
FriOI GRGCYC 1 cut(s) 49
FspBI CTAG 3 cut(s) 122, 996, 1088
GlaI GCGC 2 cut(s) 327, 329
GsuI CTGGAG 3 cut(s) 419, 605, 782
HaeIII GGCC 5 cut(s) 95, 147, 187, 347, 472
HapII CCGG 7 cut(s) 96, 469, 533, 744, 807, 819, 992
HhaI GCGC 2 cut(s) 328, 330
Hin1II CATG 5 cut(s) 136, 311, 363, 952, 1096
Hin6I GCGC 2 cut(s) 326, 328
HinP1I GCGC 2 cut(s) 326, 328
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HinfI GANTC 5 cut(s) 166, 589, 690, 883, 945
HpaI GTTAAC 1 cut(s) 804
HpaII CCGG 7 cut(s) 96, 469, 533, 744, 807, 819, 992
Hpy166II GTNNAC 2 cut(s) 804, 891
Hpy188I TCNGA 7 cut(s) 66, 87, 165, 277, 388, 411, 676
Hpy188III TCNNGA 2 cut(s) 170, 1093
Hpy8I GTNNAC 2 cut(s) 804, 891
HpyAV CCTTC 5 cut(s) 70, 85, 274, 681, 839
HpyCH4III ACNGT 2 cut(s) 860, 895
HpyCH4IV ACGT 4 cut(s) 59, 182, 242, 370
HpyCH4V TGCA 7 cut(s) 132, 246, 416, 772, 952, 976, 1030
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 243, 422, 664, 964, 973
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 4 cut(s) 59, 182, 242, 370
Hsp92II CATG 5 cut(s) 136, 311, 363, 952, 1096
HspAI GCGC 2 cut(s) 326, 328
KroI GCCGGC 2 cut(s) 95, 991
KroNI GCCGGC 2 cut(s) 97, 993
Ksp22I TGATCA 2 cut(s) 135, 1060
KspAI GTTAAC 1 cut(s) 804
Kzo9I GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
LmnI GCTCC 2 cut(s) 400, 733
Lsp1109I GCAGC 9 cut(s) 390, 523, 566, 670, 673, 676, 964, 967, 975
LweI GCATC 4 cut(s) 412, 753, 759, 862
MaeI CTAG 3 cut(s) 122, 996, 1088
MaeII ACGT 4 cut(s) 59, 182, 242, 370
MaeIII GTNAC 2 cut(s) 116, 860
MalI GATC 6 cut(s) 137, 291, 537, 617, 678, 1062
MboI GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
MboII GAAGA 3 cut(s) 79, 100, 1082
MflI RGATCY 1 cut(s) 615
MhlI GDGCHC 2 cut(s) 49, 969
MlsI TGGCCA 1 cut(s) 187
MluCI AATT 7 cut(s) 81, 268, 379, 520, 697, 826, 833
MluNI TGGCCA 1 cut(s) 187
MlyI GAGTC 1 cut(s) 939
MmeI TCCRAC 2 cut(s) 143, 300
MnlI CCTC 9 cut(s) 6, 196, 337, 427, 456, 550, 629, 803, 915
Mox20I TGGCCA 1 cut(s) 187
Mph1103I ATGCAT 1 cut(s) 418
MroNI GCCGGC 2 cut(s) 95, 991
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 187
MseI TTAA 2 cut(s) 803, 1111
Msp20I TGGCCA 1 cut(s) 187
MspA1I CMGCKG 3 cut(s) 579, 751, 962
MspI CCGG 7 cut(s) 96, 469, 533, 744, 807, 819, 992
MspR9I CCNGG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 418
MvnI CGCG 2 cut(s) 9, 328
MwoI GCNNNNNNNGC 6 cut(s) 53, 243, 422, 664, 964, 973
NaeI GCCGGC 2 cut(s) 97, 993
NciI CCSGG 1 cut(s) 744
NdeII GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
NgoMIV GCCGGC 2 cut(s) 95, 991
NheI GCTAGC 1 cut(s) 995
NlaIII CATG 5 cut(s) 136, 311, 363, 952, 1096
NlaIV GGNNCC 2 cut(s) 467, 729
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 1 cut(s) 311
PaeR7I CTCGAG 1 cut(s) 431
PagI TCATGA 1 cut(s) 1092
PauI GCGCGC 1 cut(s) 326
PciI ACATGT 1 cut(s) 307
PctI GAATGC 1 cut(s) 418
PdiI GCCGGC 2 cut(s) 97, 993
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 166, 589, 690, 883
PleI GAGTC 1 cut(s) 939
PmaCI CACGTG 2 cut(s) 183, 243
PmlI CACGTG 2 cut(s) 183, 243
PpsI GAGTC 1 cut(s) 939
Ppu21I YACGTR 2 cut(s) 183, 243
PscI ACATGT 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 49
Psp1406I AACGTT 1 cut(s) 59
PspCI CACGTG 2 cut(s) 183, 243
PspN4I GGNNCC 2 cut(s) 467, 729
PspPI GGNCC 2 cut(s) 146, 746
PspXI VCTCGAGB 1 cut(s) 431
PsuI RGATCY 1 cut(s) 615
PteI GCGCGC 1 cut(s) 326
PvuII CAGCTG 2 cut(s) 579, 962
RsaI GTAC 6 cut(s) 227, 306, 341, 353, 932, 1067
RsaNI GTAC 6 cut(s) 226, 305, 340, 352, 931, 1066
Rsr2I CGGWCCG 1 cut(s) 746
RsrII CGGWCCG 1 cut(s) 746
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 803, 1111
Sau3AI GATC 6 cut(s) 135, 289, 535, 615, 676, 1060
Sau96I GGNCC 2 cut(s) 146, 746
ScaI AGTACT 1 cut(s) 932
SchI GAGTC 1 cut(s) 939
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 2 cut(s) 49, 969
SfaNI GCATC 4 cut(s) 412, 753, 759, 862
SfcI CTRYAG 2 cut(s) 319, 787
Sfr274I CTCGAG 1 cut(s) 431
SinI GGWCC 1 cut(s) 746
SlaI CTCGAG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 7 cut(s) 81, 268, 379, 520, 697, 826, 833
SsiI CCGC 3 cut(s) 9, 749, 850
SspMI CTAG 3 cut(s) 122, 996, 1088
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 1 cut(s) 742
TaaI ACNGT 2 cut(s) 860, 895
TaiI ACGT 4 cut(s) 62, 185, 245, 373
TaqI TCGA 7 cut(s) 49, 292, 312, 432, 688, 693, 943
TasI AATT 7 cut(s) 81, 268, 379, 520, 697, 826, 833
TatI WGTACW 2 cut(s) 225, 930
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 4 cut(s) 166, 589, 690, 883
Tru1I TTAA 2 cut(s) 803, 1111
Tru9I TTAA 2 cut(s) 803, 1111
TscAI CASTG 1 cut(s) 990
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 9 cut(s) 403, 511, 579, 658, 661, 664, 952, 955, 988
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 9 cut(s) 381, 408, 446, 486, 920, 926, 993, 1010, 1109
TspRI CASTG 1 cut(s) 990
VpaK11BI GGWCC 1 cut(s) 746
XapI RAATTY 2 cut(s) 81, 379
XceI RCATGY 1 cut(s) 311
XhoI CTCGAG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 3 cut(s) 122, 996, 1088
ZrmI AGTACT 1 cut(s) 932
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.