Rroxscaffold_2G00077230
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000326
Physical Location & Seq
Reverse (-)
837699 .. 840335
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077230.1

Sequence Viewer

Length: 744 bp
ATGGGTCGAAGTAATACAAAGTTGCCTTTGGAACTAATAAAGAATGAGAAGTCTCGCAATGTCACTTTTCGAAAGAGGAAGAAGGGATTGATGAAGAAGACGTATGAATTGAACAAGCTTTGTGATGTGCAGTGTTCTGTTATCATTTACGAGAACAAAAACGGCCAACTGGTCCGGCCGGATACGTATCCCGAAAACCCTGAAGAAGTCAAGCAAATTATTGATAGATTTGTCTCCAAATCAGCCAAAGTAAGGAAAGTTGAAAACTTGGCTGATTTTTTCGGCAAACAAATCATGCAAGTGAAGAAAGAGACTGCCAAATTGCGCCAAAAGAACAATGAAGCTCGGTTTCCTTCATGGGATGACAGGCTAGATGACTTCTCATTAGATCAATTGCTTGCTCTTTTGAAAAAACTGGAGCTCAAAATCGAAGACGTGCACAAGCATTATGATAAGCAATATGCTATTGATGACAGTATACTACAACAAACGGCCTTGTTTCCGAACAACAACGTAGACTATTCTCAGATGGTTGCGTTGAACCAATACCCTACTAGTGGTTCGATGATGTACACAAGTGATAGGGCTTTGCCTGAAGAGCAAACTAATCTCCAAGCCTTGTTTCAGAACAACCTAATCAATTATCACCAGAATTACAACTACTGTTCTATCATGATGAACAACAGAAACGGTGAAGTTGAACGAATGTTACGAGAATTACCTGAAAACAAAAAAATGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

247

Amino Acids

29.13

Weight (kDa)

9.02

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 13 - 57 1.4e-16 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000326)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18650 AT4G02235 AT4G11250 AT5G26865 AT5G26950 AT5G27050 AT5G27070 AT5G27090 AT5G27090 AT5G27580 AT5G27944 AT5G37415 AT5G37415 AT5G38740 AT5G39750 AT5G39810 AT5G40070 AT5G40120 AT5G40220 AT5G41200 AT5G55690 AT5G55690 AT5G58890 AT5G65330
fragaria_vesca FvH4_2g27520 FvH4_3g19300 FvH4_4g07700 FvH4_4g07700 FvH4_4g07700 FvH4_5g27070 FvH4_6g53490 FvH4_7g04141
malus_domestica MD02G1297800.v1.1 MD15G1430800.v1.1 MD17G1250500.v1.1
prunus_persica Prupe.2G110900_v2.0.a1 Prupe.3G148900_v2.0.a1 Prupe.3G306000_v2.0.a1 Prupe.7G030200_v2.0.a1 Prupe.7G030800_v2.0.a1 Prupe.7G030800_v2.0.a1 Prupe.7G030800_v2.0.a1 Prupe.7G031800_v2.0.a1 Prupe.7G031800_v2.0.a1 Prupe.7G032000_v2.0.a1
pyrus_communis pycom02g25070 pycom06g01170 pycom09g17250 pycom111g00930 pycom15g38080
rosa_chinensis RchiOBHm_Chr2g0175531 RchiOBHm_Chr5g0016461 RchiOBHm_Chr6g0296351
rosa_laevigata RLG00000002414 RLG00000002417 RLG00000009181 RLG00000011703 RLG00000022336 RLG00000029928 RLG00000029934
rosa_multiflora Rmu_co8046860.1_g000001 Rmu_sc0000215.1_g000011 Rmu_sc0000215.1_g000020 Rmu_sc0000379.1_g000004 Rmu_sc0001478.1_g000006 Rmu_sc0002636.1_g000029 Rmu_sc0009440.1_g000006
rosa_roxburghii Rroxscaffold_2G00077230 Rroxscaffold_3G00241440 Rroxscaffold_5G00345600 Rroxscaffold_7G00171600 Rroxscaffold_7G00178500
rosa_rugosa Rorug02G0585900 Rorug04G0025700 Rorug06G0135200 Rorug06G0261200 Rorug06G0261300 Rorug06G0261400 Rorug07G0173300
rosa_samantha Rh1AG091800 Rh1AG093000 Rh1BG072700 Rh1CG088300 Rh1CG089800 Rh1CG090200 Rh1DG095900 Rh2AG665100 Rh2BG675900 Rh2CG639100 Rh2DG689900 Rh4AG103500 Rh4BG098600 Rh4CG111800 Rh4DG095500 Rh5AG126100 Rh5CG137400 Rh5DG124800 Rh5DG124900 Rh6AG373100 Rh6BG381200 Rh6CG386500 Rh6DG374000 Rh7AG315800 Rh7BG306000 Rh7CG333200 Rh7DG314000 Rh7DG314500
rosa_wichuraiana Rw2G054520 Rw4G008380 Rw5G010930 Rw6G032530 Rw7G026670 Rw7G026760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 478, 516
AcoI YGGCCR 2 cut(s) 163, 176
AcuI CTGAAG 2 cut(s) 222, 615
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 252, 557
AgsI TTSAA 5 cut(s) 112, 263, 409, 541, 701
AhlI ACTAGT 1 cut(s) 554
AjiI CACGTC 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 606, 638
AluBI AGCT 3 cut(s) 118, 344, 421
AluI AGCT 3 cut(s) 118, 344, 421
Alw21I GWGCWC 2 cut(s) 423, 441
Alw26I GTCTC 3 cut(s) 57, 238, 305
Alw44I GTGCAC 1 cut(s) 437
AoxI GGCC 3 cut(s) 163, 176, 492
ApaLI GTGCAC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 638, 704
AsuII TTCGAA 1 cut(s) 70
AvaII GGWCC 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 441
BanII GRGCYC 1 cut(s) 423
BbsI GAAGAC 2 cut(s) 104, 438
Bbv12I GWGCWC 2 cut(s) 423, 441
BccI CCATC 1 cut(s) 523
BceAI ACGGC 2 cut(s) 178, 507
BciVI GTATCC 2 cut(s) 175, 198
BcoDI GTCTC 3 cut(s) 57, 238, 305
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 3 cut(s) 371, 555, 742
BfuI GTATCC 2 cut(s) 175, 198
Bme18I GGWCC 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 104, 438
BpmI CTGGAG 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 70
BsaAI YACGTR 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 186
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 557
Bse1I ACTGG 2 cut(s) 174, 420
Bse3DI GCAATG 1 cut(s) 64
Bse8I GATNNNNATC 1 cut(s) 186
BseGI GGATG 1 cut(s) 367
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 2 cut(s) 252, 557
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 2 cut(s) 174, 420
BseSI GKGCMC 1 cut(s) 441
BseX3I CGGCCG 1 cut(s) 176
BsgI GTGCAG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 3 cut(s) 165, 178, 494
BsiEI CGRYCG 1 cut(s) 179
BsiHKAI GWGCWC 2 cut(s) 423, 441
BsiSI CCGG 2 cut(s) 175, 179
BslI CCNNNNNNNGG 2 cut(s) 252, 557
BsmAI GTCTC 3 cut(s) 57, 238, 305
BsnI GGCC 3 cut(s) 165, 178, 494
Bsp119I TTCGAA 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 423, 441
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 1 cut(s) 388
BspANI GGCC 3 cut(s) 165, 178, 494
BspCNI CTCAG 1 cut(s) 538
BspHI TCATGA 1 cut(s) 672
BspQI GCTCTTC 1 cut(s) 591
BspT104I TTCGAA 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 64
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 2 cut(s) 174, 420
BssMI GATC 1 cut(s) 388
BssNAI GTATAC 1 cut(s) 479
Bst1107I GTATAC 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 476, 665, 692
Bst6I CTCTTC 1 cut(s) 591
BstAUI TGTACA 1 cut(s) 570
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 367
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 3 cut(s) 57, 238, 305
BstMBI GATC 1 cut(s) 388
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstSLI GKGCMC 1 cut(s) 441
BstSNI TACGTA 1 cut(s) 186
BstV2I GAAGAC 2 cut(s) 104, 438
BstZ17I GTATAC 1 cut(s) 479
BstZI CGGCCG 1 cut(s) 176
BsuI GTATCC 2 cut(s) 175, 198
BsuRI GGCC 3 cut(s) 165, 178, 494
BtrI CACGTC 1 cut(s) 436
BtsCI GGATG 1 cut(s) 367
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 399
CciI TCATGA 1 cut(s) 672
CfoI GCGC 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 571
CviAII CATG 3 cut(s) 295, 357, 673
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 1 cut(s) 525
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
EaeI YGGCCR 2 cut(s) 163, 176
EagI CGGCCG 1 cut(s) 176
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Ecl136II GAGCTC 1 cut(s) 421
EclXI CGGCCG 1 cut(s) 176
Eco105I TACGTA 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 172
Eco52I CGGCCG 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 421
Eco57I CTGAAG 2 cut(s) 222, 615
EcoICRI GAGCTC 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 423
FaeI CATG 3 cut(s) 298, 360, 676
FaiI YATR 7 cut(s) 105, 296, 358, 450, 462, 479, 674
FatI CATG 3 cut(s) 294, 356, 672
FblI GTMKAC 2 cut(s) 478, 516
FokI GGATG 1 cut(s) 374
FriOI GRGCYC 1 cut(s) 423
FspBI CTAG 3 cut(s) 371, 555, 742
GlaI GCGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 3 cut(s) 165, 178, 494
HapII CCGG 2 cut(s) 175, 179
HhaI GCGC 1 cut(s) 327
Hin1II CATG 3 cut(s) 298, 360, 676
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 116
HpaII CCGG 2 cut(s) 175, 179
HphI GGTGA 2 cut(s) 638, 704
Hpy166II GTNNAC 4 cut(s) 439, 479, 517, 573
Hpy188I TCNGA 3 cut(s) 504, 528, 627
Hpy188III TCNNGA 2 cut(s) 191, 673
Hpy8I GTNNAC 4 cut(s) 439, 479, 517, 573
HpyAV CCTTC 2 cut(s) 76, 363
HpyCH4III ACNGT 3 cut(s) 476, 665, 692
HpyCH4IV ACGT 4 cut(s) 101, 185, 435, 513
HpyCH4V TGCA 3 cut(s) 130, 298, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 4 cut(s) 101, 185, 435, 513
Hsp92II CATG 3 cut(s) 298, 360, 676
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 1 cut(s) 388
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 1 cut(s) 418
LpnPI CCDG 9 cut(s) 155, 188, 192, 213, 352, 401, 606, 662, 735
MaeI CTAG 3 cut(s) 371, 555, 742
MaeII ACGT 4 cut(s) 101, 185, 435, 513
MaeIII GTNAC 2 cut(s) 61, 708
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 7 cut(s) 91, 106, 109, 215, 316, 443, 608
MfeI CAATTG 1 cut(s) 392
MhlI GDGCHC 2 cut(s) 423, 441
MluCI AATT 7 cut(s) 107, 216, 320, 392, 640, 652, 716
MnlI CCTC 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 2 cut(s) 175, 179
MunI CAATTG 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 598
NdeII GATC 1 cut(s) 388
NlaIII CATG 3 cut(s) 298, 360, 676
NmuCI GTSAC 1 cut(s) 61
NspV TTCGAA 1 cut(s) 70
PagI TCATGA 1 cut(s) 672
PciSI GCTCTTC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 709
Ppu21I YACGTR 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 172
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 591
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 1 cut(s) 172
SduI GDGCHC 2 cut(s) 423, 441
SetI ASST 9 cut(s) 104, 120, 188, 346, 423, 438, 516, 636, 724
SfuI TTCGAA 1 cut(s) 70
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 299
SnaBI TACGTA 1 cut(s) 186
SpeI ACTAGT 1 cut(s) 554
Sse9I AATT 7 cut(s) 107, 216, 320, 392, 640, 652, 716
SspMI CTAG 3 cut(s) 371, 555, 742
SstI GAGCTC 1 cut(s) 423
TaaI ACNGT 3 cut(s) 476, 665, 692
TaiI ACGT 4 cut(s) 104, 188, 438, 516
TaqI TCGA 4 cut(s) 7, 70, 429, 563
TasI AATT 7 cut(s) 107, 216, 320, 392, 640, 652, 716
TatI WGTACW 1 cut(s) 570
TscAI CASTG 1 cut(s) 137
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 5 cut(s) 107, 120, 345, 354, 692
TspRI CASTG 1 cut(s) 137
VneI GTGCAC 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 172
XmiI GTMKAC 2 cut(s) 478, 516
XspI CTAG 3 cut(s) 371, 555, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.