MD04G1053100.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000416
Physical Location & Seq
Reverse (-)
6245864 .. 6250895
5032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1053100.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGAAGTGTTTCTTTTATTTCAAGGAAAAATGCAAGAGCAAAAGAGAAGGATCACAATCCGCTCCAGAATTGAGAAACAGAAGCAATCCGGTTTTGGATCGCACAACGAAATCTTCGAGTTCAGTGCCATCTCCGAAGAGCATACCAGAATTGTACAGAGAAAAGGAGCAGAATTTGAGAGTTTTCTCCCTCCAAGAGCTGCGGGAAGCAACAAATAGTTTCAACAGGTTGCTTAAGCTCGGGGAAGGAGGTTTTGGGAGTGTTTATAAAGGAACAATCAGGCCTGCCGATGGAGAAAGGGGTAGTCCGATTTTGGTTGCCATCAAGAAGTTAAACCCTCAGAGTTTACAGGGTCATAAAGAATGGCTTGCGGAAGTTCAATTTCTTGGCGTCGTAGAGCACCCAAATCTGGTAAAACTTTTAGGTTACTGTTCTGTAGATGGAGAAAGAAGGATACAACGGTTGTTGGTGTATGAATTTGTGCCTAATAGAAGCTTAGAAGACCATCTTTTCAACAGGGCTTTGCCCACATTGCCTTGGATAACAAGATTACAGATTATGCTTGGTGCTGCTCAAGGATTGGCTTATCTGCACATGGGACTCGAAGTCCAGGTAATATTTCGAGATTTCAAATCCTCAAATGTGCTATTGGATGAAGAATTTAGGCCAAAGCTCTCAGACTTCGGGCTTGCTAGAGAAGGACCAACGGATGACCGTAGTCATGTATCAACCTCGGTGGTGGGGACTTACGGATACGCAGCCCCAGAATATATTGAAACGGGCCATCTCTCAACTAGTAGTGACTTGTGGAGTTTTGGTGTGGTGCTGTACGAGATCCTAACAGGCAGGCGTGTCTTAGACAAGAACCGCCCTACAGCAGAACAGAAGCTTCTTTACTGGGTTAGACAGTTCCCTGCTGACAGTAAAAGGTTCAGCATGATAATAGATCCCCTACTGAGAGACCAGTACTCTCTTACTGCAGCTCGGAATGTTGCCAAGTTGGCCGATAGCTGTCTGAACAAGAACGCAAAGGAACGGCCAACAATGAGTCAGGTGGTAGAGGTGTTGGAGCAAGCTCTACAAGATTCACAAGGGAGCACCAGTTCTTCAAAGACAAGTTCTGAGGCATCTACGTCCAAATCGGCGAAGCGGAGACCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

388

Amino Acids

43.65

Weight (kDa)

9.43

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 75 - 354 1.7e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 79 - 356 5.9e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47070
fragaria_vesca FvH4_5g20770 FvH4_5g20770 FvH4_5g20770 FvH4_5g20770 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930 FvH4_7g08930
malus_domestica MD02G1222400.v1.1 MD04G1053100.v1.1 MD06G1044400.v1.1 MD07G1093200.v1.1
prunus_persica Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.2G118500_v2.0.a1 Prupe.5G052200_v2.0.a1 Prupe.5G052200_v2.0.a1
pyrus_communis pycom02g18890 pycom04g04660 pycom06g03780 pycom07g07640
rosa_chinensis RchiOBHm_Chr0c22g0500461 RchiOBHm_Chr0c22g0500491 RchiOBHm_Chr0c22g0500531 RchiOBHm_Chr0c22g0500541 RchiOBHm_Chr0c22g0500641 RchiOBHm_Chr0c22g0500691 RchiOBHm_Chr1g0344381 RchiOBHm_Chr6g0259661 RchiOBHm_Chr6g0259701 RchiOBHm_Chr7g0205471 RchiOBHm_Chr7g0206561 RchiOBHm_Chr7g0206611 RchiOBHm_Chr7g0206731 RchiOBHm_Chr7g0206741 RchiOBHm_Chr7g0206781 RchiOBHm_Chr7g0206801 RchiOBHm_Chr7g0206811 RchiOBHm_Chr7g0206831 RchiOBHm_Chr7g0206861 RchiOBHm_Chr7g0206891 RchiOBHm_Chr7g0206981 RchiOBHm_Chr7g0206991 RchiOBHm_Chr7g0207071 RchiOBHm_Chr7g0207121 RchiOBHm_Chr7g0207151 RchiOBHm_Chr7g0207181 RchiOBHm_Chr7g0207241 RchiOBHm_Chr7g0207251 RchiOBHm_Chr7g0207261 RchiOBHm_Chr7g0207411 RchiOBHm_Chr7g0207471 RchiOBHm_Chr7g0207701
rosa_laevigata RLG00000003296 RLG00000003302 RLG00000003326 RLG00000003346 RLG00000028901
rosa_multiflora Rmu_sc0000536.1_g000001 Rmu_sc0000536.1_g000002 Rmu_sc0004987.1_g000003 Rmu_sc0008509.1_g000002 Rmu_sc0008509.1_g000029 Rmu_sc0012558.1_g000001 Rmu_sc0013160.1_g000002 Rmu_sc0014912.1_g000004
rosa_roxburghii Rroxscaffold_3G00251310 Rroxscaffold_3G00251320 Rroxscaffold_3G00251410 Rroxscaffold_3G00252190 Rroxscaffold_4G00309810 Rroxscaffold_7G00206970 Rroxscaffold_7G00207090
rosa_rugosa Rorug01G0173500 Rorug01G0173600 Rorug05G0590200 Rorug07G0096400 Rorug07G0096700 Rorug07G0096900 Rorug07G0098500 Rorug07G0099000
rosa_samantha Rh1AG189700 Rh1BG156700 Rh1DG188100 Rh7BG221600 Rh7BG225000 Rh7BG225300 Rh7BG227500 Rh7BG227700 Rh7CG244800
rosa_wichuraiana Rw1G015650 Rw6G009210 Rw7G019820 Rw7G019850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 62
AciI CCGC 5 cut(s) 60, 202, 371, 868, 1150
AclWI GGATC 4 cut(s) 58, 105, 829, 941
AcoI YGGCCR 2 cut(s) 1002, 1037
AcsI RAATTY 3 cut(s) 172, 476, 659
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 3 cut(s) 155, 830, 968
AfiI CCNNNNNNNGG 2 cut(s) 290, 409
AflII CTTAAG 1 cut(s) 233
AgsI TTSAA 7 cut(s) 22, 223, 380, 514, 631, 776, 1110
AhdI GACNNNNNGTC 1 cut(s) 605
AhlI ACTAGT 1 cut(s) 794
AjnI CCWGG 1 cut(s) 609
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AloI GAACNNNNNNTCC 2 cut(s) 1087, 1119
AluBI AGCT 8 cut(s) 199, 238, 495, 673, 889, 983, 1011, 1076
AluI AGCT 8 cut(s) 199, 238, 495, 673, 889, 983, 1011, 1076
Alw21I GWGCWC 2 cut(s) 402, 1100
Alw26I GTCTC 2 cut(s) 954, 1147
AlwI GGATC 4 cut(s) 58, 105, 829, 941
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 5 cut(s) 281, 665, 781, 1002, 1037
ApeKI GCWGC 4 cut(s) 199, 569, 758, 980
ApoI RAATTY 3 cut(s) 172, 476, 659
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 701, 781
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 1 cut(s) 701
BaeI ACNNNNGTAYC 2 cut(s) 446, 479
BarI GAAGNNNNNNTAC 2 cut(s) 878, 910
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 2 cut(s) 402, 1100
BbvI GCAGC 4 cut(s) 186, 556, 770, 992
BccI CCATC 6 cut(s) 136, 284, 329, 434, 513, 792
BceAI ACGGC 1 cut(s) 1052
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 611
BciVI GTATCC 2 cut(s) 447, 746
BcoDI GTCTC 2 cut(s) 954, 1147
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 2 cut(s) 693, 795
BfmI CTRYAG 3 cut(s) 435, 873, 978
BfrI CTTAAG 1 cut(s) 233
BfuI GTATCC 2 cut(s) 447, 746
BglI GCCNNNNNGGC 1 cut(s) 1001
BisI GCNGC 4 cut(s) 200, 570, 759, 981
BlsI GCNGC 4 cut(s) 201, 571, 760, 982
BmcAI AGTACT 1 cut(s) 968
Bme1390I CCNGG 1 cut(s) 611
Bme18I GGWCC 1 cut(s) 701
BmeRI GACNNNNNGTC 1 cut(s) 605
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 701, 781
BmrFI CCNGG 1 cut(s) 611
BmrI ACTGGG 1 cut(s) 907
BmsI GCATC 1 cut(s) 1136
BmuI ACTGGG 1 cut(s) 907
BoxI GACNNNNGTC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 2 cut(s) 954, 1147
BsaJI CCNNGG 2 cut(s) 536, 732
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 4 cut(s) 802, 832, 1087, 1117
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 409
Bse1I ACTGG 3 cut(s) 902, 964, 1101
Bse3DI GCAATG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 55
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 536, 732
BseGI GGATG 2 cut(s) 658, 715
BseJI GATNNNNATC 1 cut(s) 55
BseLI CCNNNNNNNGG 2 cut(s) 290, 409
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 4 cut(s) 353, 690, 947, 1113
BseNI ACTGG 3 cut(s) 902, 964, 1101
BseXI GCAGC 4 cut(s) 186, 556, 770, 992
BsgI GTGCAG 1 cut(s) 575
BshFI GGCC 5 cut(s) 283, 667, 783, 1004, 1039
BsiHKAI GWGCWC 2 cut(s) 402, 1100
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 1 cut(s) 89
BslFI GGGAC 2 cut(s) 612, 757
BslI CCNNNNNNNGG 2 cut(s) 290, 409
BsmAI GTCTC 2 cut(s) 954, 1147
BsmFI GGGAC 2 cut(s) 612, 757
BsnI GGCC 5 cut(s) 283, 667, 783, 1004, 1039
Bso31I GGTCTC 2 cut(s) 954, 1147
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 402, 1100
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 4 cut(s) 50, 97, 834, 946
BspACI CCGC 5 cut(s) 60, 202, 371, 868, 1150
BspANI GGCC 5 cut(s) 283, 667, 783, 1004, 1039
BspCNI CTCAG 4 cut(s) 352, 689, 948, 1114
BspMAI CTGCAG 1 cut(s) 982
BspPI GGATC 4 cut(s) 58, 105, 829, 941
BspQI GCTCTTC 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 233
BspTNI GGTCTC 2 cut(s) 954, 1147
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 530
BsrGI TGTACA 1 cut(s) 153
BsrI ACTGG 3 cut(s) 902, 964, 1101
BssECI CCNNGG 2 cut(s) 536, 732
BssMI GATC 4 cut(s) 50, 97, 834, 946
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 536
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 5 cut(s) 431, 462, 716, 909, 923
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 233
BstAUI TGTACA 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 285, 369, 690, 848, 1074
BstDEI CTNAG 6 cut(s) 339, 496, 676, 856, 956, 1122
BstF5I GGATG 2 cut(s) 658, 715
BstKTI GATC 4 cut(s) 53, 100, 837, 949
BstMAI GTCTC 2 cut(s) 954, 1147
BstMBI GATC 4 cut(s) 50, 97, 834, 946
BstMWI GCNNNNNNNGC 2 cut(s) 532, 1001
BstNI CCWGG 1 cut(s) 611
BstPAI GACNNNNGTC 1 cut(s) 717
BstSCI CCNGG 1 cut(s) 609
BstSFI CTRYAG 3 cut(s) 435, 873, 978
BstV1I GCAGC 4 cut(s) 186, 556, 770, 992
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 2 cut(s) 834, 946
BstYI RGATCY 2 cut(s) 834, 946
BsuI GTATCC 2 cut(s) 447, 746
BsuRI GGCC 5 cut(s) 283, 667, 783, 1004, 1039
BtsCI GGATG 2 cut(s) 658, 715
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 5 cut(s) 285, 369, 690, 848, 1074
Cfr13I GGNCC 2 cut(s) 701, 781
CseI GACGC 1 cut(s) 379
Csp6I GTAC 3 cut(s) 154, 829, 967
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 3 cut(s) 595, 722, 937
CviQI GTAC 3 cut(s) 154, 829, 967
DdeI CTNAG 6 cut(s) 339, 496, 676, 856, 956, 1122
DpnI GATC 4 cut(s) 52, 99, 836, 948
DpnII GATC 4 cut(s) 50, 97, 834, 946
DriI GACNNNNNGTC 1 cut(s) 605
EaeI YGGCCR 2 cut(s) 1002, 1037
Eam1104I CTCTTC 1 cut(s) 131
Eam1105I GACNNNNNGTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 536
Eco147I AGGCCT 1 cut(s) 283
Eco31I GGTCTC 2 cut(s) 954, 1147
Eco47I GGWCC 1 cut(s) 701
Eco88I CYCGRG 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 609
EcoT14I CCWWGG 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 536
FaeI CATG 3 cut(s) 598, 725, 940
FaiI YATR 9 cut(s) 143, 267, 357, 474, 560, 596, 723, 771, 938
FalI AAGNNNNNCTT 5 cut(s) 28, 351, 383, 492, 524
FaqI GGGAC 2 cut(s) 612, 757
FatI CATG 3 cut(s) 594, 721, 936
FauI CCCGC 1 cut(s) 195
Fnu4HI GCNGC 4 cut(s) 200, 570, 759, 981
FokI GGATG 2 cut(s) 665, 722
Fsp4HI GCNGC 4 cut(s) 200, 570, 759, 981
FspBI CTAG 2 cut(s) 693, 795
GluI GCNGC 4 cut(s) 200, 570, 759, 981
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 5 cut(s) 283, 667, 783, 1004, 1039
HapII CCGG 1 cut(s) 89
HgaI GACGC 1 cut(s) 379
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 3 cut(s) 598, 725, 940
HindIII AAGCTT 2 cut(s) 493, 887
HinfI GANTC 3 cut(s) 600, 1048, 1085
HpaII CCGG 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 7 cut(s) 135, 309, 342, 679, 987, 1017, 1123
Hpy188III TCNNGA 3 cut(s) 65, 325, 623
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 4 cut(s) 41, 239, 444, 692
HpyCH4III ACNGT 5 cut(s) 431, 462, 716, 909, 923
HpyCH4IV ACGT 1 cut(s) 1133
HpyCH4V TGCA 3 cut(s) 33, 592, 980
HpyF10VI GCNNNNNNNGC 2 cut(s) 532, 1001
HpyF3I CTNAG 6 cut(s) 339, 496, 676, 856, 956, 1122
HpySE526I ACGT 1 cut(s) 1133
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 3 cut(s) 598, 725, 940
Kzo9I GATC 4 cut(s) 50, 97, 834, 946
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 4 cut(s) 67, 166, 1069, 1095
Lsp1109I GCAGC 4 cut(s) 186, 556, 770, 992
LweI GCATC 1 cut(s) 1136
MaeI CTAG 2 cut(s) 693, 795
MaeII ACGT 1 cut(s) 1133
MaeIII GTNAC 2 cut(s) 425, 800
MalI GATC 4 cut(s) 52, 99, 836, 948
MbiI CCGCTC 1 cut(s) 62
MboI GATC 4 cut(s) 50, 97, 834, 946
MboII GAAGA 5 cut(s) 105, 148, 512, 668, 1098
MflI RGATCY 2 cut(s) 834, 946
MhlI GDGCHC 2 cut(s) 402, 1100
MluCI AATT 6 cut(s) 68, 149, 172, 380, 476, 659
MlyI GAGTC 2 cut(s) 594, 1057
MmeI TCCRAC 1 cut(s) 1047
MnlI CCTC 7 cut(s) 200, 242, 348, 646, 742, 1054, 1117
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 234, 332
MspCI CTTAAG 1 cut(s) 233
MspI CCGG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 611
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 2 cut(s) 532, 1001
NdeII GATC 4 cut(s) 50, 97, 834, 946
NlaIII CATG 3 cut(s) 598, 725, 940
NmuCI GTSAC 1 cut(s) 800
PceI AGGCCT 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 1085
PkrI GCNGC 4 cut(s) 201, 571, 760, 982
PleI GAGTC 2 cut(s) 594, 1056
PpsI GAGTC 2 cut(s) 594, 1056
PshAI GACNNNNGTC 1 cut(s) 717
PsiI TTATAA 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspPI GGNCC 2 cut(s) 701, 781
PstI CTGCAG 1 cut(s) 982
PsuI RGATCY 2 cut(s) 834, 946
RsaI GTAC 3 cut(s) 155, 830, 968
RsaNI GTAC 3 cut(s) 154, 829, 967
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 234, 332
SatI GCNGC 4 cut(s) 200, 570, 759, 981
Sau3AI GATC 4 cut(s) 50, 97, 834, 946
Sau96I GGNCC 2 cut(s) 701, 781
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 2 cut(s) 594, 1057
ScrFI CCNGG 1 cut(s) 611
SduI GDGCHC 2 cut(s) 402, 1100
SfaNI GCATC 1 cut(s) 1136
SfcI CTRYAG 3 cut(s) 435, 873, 978
SinI GGWCC 1 cut(s) 701
SmlI CTYRAG 2 cut(s) 233, 573
SmoI CTYRAG 2 cut(s) 233, 573
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 6 cut(s) 68, 149, 172, 380, 476, 659
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 5 cut(s) 60, 202, 371, 868, 1150
SspI AATATT 1 cut(s) 618
SspMI CTAG 2 cut(s) 693, 795
StuI AGGCCT 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 609
StyI CCWWGG 1 cut(s) 536
TaaI ACNGT 5 cut(s) 431, 462, 716, 909, 923
TaiI ACGT 1 cut(s) 1136
TaqI TCGA 3 cut(s) 116, 603, 622
TasI AATT 6 cut(s) 68, 149, 172, 380, 476, 659
TatI WGTACW 2 cut(s) 153, 966
TfiI GAWTC 1 cut(s) 1085
Tru1I TTAA 2 cut(s) 234, 332
Tru9I TTAA 2 cut(s) 234, 332
TscAI CASTG 1 cut(s) 129
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 4 cut(s) 199, 569, 758, 980
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 3 cut(s) 17, 489, 669
TspGWI ACGGA 2 cut(s) 722, 765
TspRI CASTG 1 cut(s) 129
Vha464I CTTAAG 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 701
XapI RAATTY 3 cut(s) 172, 476, 659
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 693, 795
ZrmI AGTACT 1 cut(s) 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.